Procaryotic Micro-organism Patents (Class 435/71.2)
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Patent number: 6727353Abstract: The invention provides isolated nucleic acid and amino acid sequences of Slo potassium family members such as, antibodies to Kv10 subfamily members such as Kv10.1, methods of detecting Kv10, subfamily members such as Kv10.1, methods of screening for potassium channel activators and inhibitors using biologically active Kv10 subfamily members such as Kv10.1, and kits for screening for activators and inhibitors of voltage-gated potassium channels comprising Kv10 subfamily members such as Kv10.1.Type: GrantFiled: April 11, 2001Date of Patent: April 27, 2004Assignee: Icagen, Inc.Inventor: Timothy James Jegla
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Patent number: 6716603Abstract: A new gene in the calcium-activated chloride channel family has been discovered that is induced by IL-9, thereby providing a therapeutic target in IL-9 mediated development of atopic allergy, asthma-related disorders and cystic fibrosis. A method for the identification and use of small molecule inhibitors of this gene and its products to treat these disorders has also been discovered. The invention also includes a method for diagnosing susceptibility to, and assessing treatment of atopic allergy, asthma-related disorders by measuring the level of gene expression in biologic samples using antibody specific for this protein.Type: GrantFiled: October 16, 2002Date of Patent: April 6, 2004Assignee: Genaera CorporationInventors: Kenneth J. Holroyd, Roy C. Levitt, W. Lee Maloy, Jamila Louahed, Mike McLane, Nicholas C. Nicolaides, Yuhong Zhou, Qu Dong
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Patent number: 6716602Abstract: The invention provides a method for increasing product yield of a polypeptide of interest produced by recombinant host cells, where expression of the polypeptide by the recombinant host cells is regulated by an inducible system. More specifically, the method involves culturing the recombinant host cells under conditions of high metabolic and growth rate and then reducing the metabolic rate of the recombinant host cells at the time of induction of polypeptide expression. In particular, the invention provides a method of increasing product yield of an antibody, growth factor, or protease produced by a recombinant E. coli host cell regulated by an inducible system.Type: GrantFiled: November 1, 2001Date of Patent: April 6, 2004Assignee: Genentech, Inc.Inventors: Dana Andersen, John Joly, Bradley R. Snedecor
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Patent number: 6703495Abstract: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications.Type: GrantFiled: June 6, 2001Date of Patent: March 9, 2004Assignee: Lexicon Genetics IncorporatedInventors: D. Wade Walke, John Scoville
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Publication number: 20040033567Abstract: A method for the production of an enzyme of interest, on an industrial scale, comprisingType: ApplicationFiled: June 24, 2003Publication date: February 19, 2004Applicant: Novozymes A/SInventors: Mogens Wumpelmann, Niels Banke, Soren Michaelsen
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Patent number: 6692735Abstract: The present invention relates to a variant of Lkn-1(shLkn-1) with enhanced biological activity, which is a truncated form of Lkn-1, a process for preparing a recombinant shLkn-1 by employing expression vector therefor and pharmaceutical application of the said protein. shLkn-1 is generated by missing 26 amino acid residues from the amino terminus of Lkn-1 to contain 66 amino acids. Recombinant shLkn-1 inhibits colony formation and cell proliferation in vivo, which suggests that it can be used as a potential drug for the antibody production, the treatment during HIV-1 infection, the protection of bone marrow stem cells during chemotherapy or radiotherapy, and the inhibition of leukemia.Type: GrantFiled: July 21, 1999Date of Patent: February 17, 2004Assignees: Korea Green Cross Corporation, Mogam Biotechnology Research InstituteInventors: Byoung S. Kwon, Byung S. Youn, Soo-Il Chung, Doo-Hong Park, Seung Jae Baek, Eun-Kyoung Lee, Ju-Hyung Ahn, Kong-Ju Lee
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Publication number: 20040029233Abstract: The invention relates to a method for producing biomass from halophilic organisms, in which the halophilic organisms are fermented in a hollow space in a salt dome and said halophilic organisms or components thereof are isolated as biomass.Type: ApplicationFiled: June 13, 2003Publication date: February 12, 2004Inventor: Norbert Hampp
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Patent number: 6689604Abstract: The structure and specificity of a recombinant &agr;2,3-sialyltransferase from Campylobacter spp., is disclosed. Also provided are methods for using the &agr;2,3-sialyltransferase in the production of desired carbohydrate structures and nucleic acids that encode the sialyltransferase.Type: GrantFiled: March 18, 1999Date of Patent: February 10, 2004Assignee: National Research Council of CanadaInventors: Michel Gilbert, Warren W. Wakarchuk
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Patent number: 6680380Abstract: Nucleic acids encoding mammalian, e.g., primate, IL-1&zgr;, purified IL-1&zgr; polypeptides and fragments thereof. Binding proteins, e.g., antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositions for both diagnostic and therapeutic utilities are provided.Type: GrantFiled: September 17, 1999Date of Patent: January 20, 2004Assignee: Schering CorporationInventor: Jacqueline C. Timans
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Patent number: 6677442Abstract: The present invention relates to a human cDNA homologous to the yeast REV1 gene. The sequence of human REV1 (hREV1) gene is described.Type: GrantFiled: October 30, 2000Date of Patent: January 13, 2004Assignee: University of Kentucky Research FoundationInventors: Zhigang Wang, Wensheng Winston Lin, Hua Xin, Xiaohua Wu
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Patent number: 6673915Abstract: Substantially pure nucleic acid molecules encoding the monocyte chemotactic proteins MCP-4 and MCP-5. These molecules and the polypeptides they encode are useful in treating diseases or conditions that: (1) are exacerbated by a local immune response, (2) would benefit from a local immune response, or (3) are caused by infectious agents that gain entry to mammalian cells via the chemokine receptors bound by MCP-4 or MCP-5.Type: GrantFiled: April 7, 2000Date of Patent: January 6, 2004Assignee: General Hospital CorporationInventors: Andrew D. Luster, Eduardo A. Garcia-Zepeda, Mindy N. Sarafi
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Patent number: 6660263Abstract: Oocydin A is a mono-chlorinated lipophilic macrocyclic lactone that was isolated from a strain of Serratia marcescens that produces oocydin A in culture. Oocydin A has a molecular mass of 470, contains one atom of chlorine, a carboxyl group, and a tetrahydrofuran ring internal to a larger macrocyclic ring. Minimum inhibitory concentrations of circa 0.03 &mgr;g ml−1 were noted for oocydin A against phytopathogenic Oomycetes. Oocydin A can be used as an anti-Oomycete in agricultural applications for crop protection.Type: GrantFiled: May 17, 2001Date of Patent: December 9, 2003Assignee: HMV CorporationInventors: Gary A. Strobel, Jia Yao Li
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Patent number: 6653068Abstract: The present invention relates to the generation of specific binding partners binding to (poly)peptides encoded by genomic DNA fragments or ESTs. The (poly)peptides are expressed as part of fusion proteins which are forming inclusion bodies on expression in host cells. The inclusion bodies are used to generate binding partners which bind specifically to said (poly)peptides. The specific binding partners, in particular immunoglobulins or fragments thereof, are useful for analysis and functional characterization of proteins encoded by nucleic acid sequences comprising the corresponding genomic DNA fragments or ESTs. The invention further relates to nucleic acid molecules, vectors and host cells to be used in the methods of the present invention.Type: GrantFiled: February 28, 2001Date of Patent: November 25, 2003Assignee: Morphosys AGInventors: Christian Frisch, Titus Kretzschmar, Adolf Höss, Thomas Von Rüden
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Patent number: 6645738Abstract: The present invention relates to rhesus monkey DNA molecules encoding the melanocortin-5 receptor protein, recombinant vectors comprising DNA molecules encoding rhesus MC-5R, recombinant host cells which contain a recombinant vectors encoding rhesus MC-5R, the rhesus MC-5R protein encoded by the DNA molecule, and methods of identifying selective agonists and antagonists of rhesus MC-5R.Type: GrantFiled: May 4, 2001Date of Patent: November 11, 2003Assignee: Merck & Co., Inc.Inventors: Tung M. Fong, Leonardus H. T. Van Der Ploeg, Ruey-Ruey C. Huang
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Publication number: 20030206920Abstract: A protein product extracted from apathogen bacilli is described.Type: ApplicationFiled: May 2, 2002Publication date: November 6, 2003Inventor: Rafael Chacon Pabon
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Patent number: 6642026Abstract: The gene of human acidic fibroblast growth factor 155 (haFGF 155) has been obtained by chemical synthesis. The nucleotide sequence of haFGF 155 gene has been deduced on the basis of haFGF 155 amino acid sequence as described in the literature. The amino acid sequence of the synthesized haFGF 155 does not differ from those described in the literature. The nucleotide sequence of haFGF gene differs from those described previously. For chemical synthesis of haFGF 155 gene, codons were used which are the ones most often used by E. coli in highly expressed E. coli proteins. A plasmid with haPGF 155 (phaFGF 155) gene was obtained and was used to transform E. coli. Production of haFGF 154 protein was achieved by cultivation of the producer strain under conditions which slow down the lytic development of lambda phage. The haFGF 154 protein accumulated in culture medium in a soluble condition as a result of the producer strain cells lysis by the lambda phage.Type: GrantFiled: August 15, 2001Date of Patent: November 4, 2003Assignee: Phage Biotechnology CorporationInventors: Thomas J. Stegmann, Vitaliy A. Kordyum, Iryna Yu. Slavchenko, Svitlana I. Chernykh, Oleksandr F. Vozianov
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Patent number: 6642030Abstract: Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis &dgr;-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.Type: GrantFiled: October 27, 1999Date of Patent: November 4, 2003Assignee: Monsanto Technology, LLCInventors: Leigh H. English, Susan M. Brussock, Thomas M. Malvar, James W. Bryson, Caroline A. Kulesza, Frederick S. Walters, Stephen L. Slatin, Michael A. Von Tersch, Charles Romano
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Patent number: 6642025Abstract: In summary of this disclosure, the present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector, containing a nucleotide sequence encoding a lorf2 protein of a strain of Chlamydia pneumoniae and a promoter to effect expression of the lorf2 gene in the host. Modifications are possible within the scope of this invention.Type: GrantFiled: July 13, 2001Date of Patent: November 4, 2003Assignee: Aventis Pasteur LimitedInventors: Andrew D. Murdin, Raymond P. Oomen, Pamela L. Dunn
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Patent number: 6638733Abstract: The invention provides isolated nucleic acid and amino acid sequences of four novel G-protein coupled receptors that are amplified in breast cancer cells, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of G-protein coupled receptors.Type: GrantFiled: March 14, 2000Date of Patent: October 28, 2003Assignee: Tularik Inc.Inventors: Scott Powers, Jianxin Yang, Gene Cutler
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Patent number: 6638503Abstract: The present invention relates to a novel microorganism Streptomyces megasporus SD5. The present invention also relates to a process for the isolation of said Streptomyces megasporus SD5. The invention also relates to a novel fibrinolytic enzyme actinokinase extracted from said microorganism and to a process for the extraction of said enzyme. In another aspect, the invention also pertains to a method for the treatment of thrombolytic disorders using said enzyme.Type: GrantFiled: November 28, 2001Date of Patent: October 28, 2003Assignee: Maharashtra Association for the Cultivation of ScienceInventors: Ratnakar Ravindra Chitte, Sabita Dey
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Patent number: 6635741Abstract: The invention provides isolated nucleic acid and amino acid sequences of four novel G-protein coupled receptors that are amplified in breast cancer cells, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of G-protein coupled receptors.Type: GrantFiled: April 11, 2000Date of Patent: October 21, 2003Assignee: Tularik Inc.Inventors: Scott Powers, Jianxin Yang, Gene Cutler
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Publication number: 20030195146Abstract: (Beauveria) sp. FO-6979 (FERM BP-6681), which belongs to the genus Beauveria and is capable of producing FO-6979-M0, -M1, -M2, -M3 and -M4 substances, is cultured in a medium to thereby accumulate the FO-6979-M0, -M1, -M2, -M3 and -M4 substances in the liquid culture medium. Then the FO-6979-M0, -M1, -M2, -M3 and -M4 substances are collected from the culture medium. The substances thus obtained are less toxic, specifically inhibit acyl-Coenzyme A: cholesterol acyltransferase, and inhibit the formation of oil droplets in macrophages. Owing to these characteristics, the above substances are useful in preventing and treating human diseases caused by cholesterol accumulation.Type: ApplicationFiled: May 28, 2003Publication date: October 16, 2003Inventors: Satoshi Omura, Hiroshi Tomoda
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Patent number: 6632638Abstract: Disclosed are methods for improving the solubility of a protein of interest produced recombinantly by expressing the protein of interest as a fusion protein with Uracil DNA glycosylase inhibitor (UGI).Type: GrantFiled: November 17, 2000Date of Patent: October 14, 2003Assignee: Amgen, Inc.Inventors: Marshall Snavely, Lana Klionsky
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Publication number: 20030186388Abstract: Depsipeptides and congeners thereof are disclosed having structure (I), wherein m, n, p, q, X, R1, R2 and R3 are as defined herein. These compounds, including FR901228, have activity as, for example, immunosuppressants, as well as for the prevention or treatment of patients suffering or at risk of suffering from inflammatory, autoimmune or immune system-related diseases including graft-versus-host disease and enhancement of graft/tissue survival following transplant. Also provided are methods for inhibiting lymphocyte activation, proliferation, and/or suppression of IL-2 secretion.Type: ApplicationFiled: February 27, 2003Publication date: October 2, 2003Inventors: Satoshi Ueda, Yoko Watamoto, Masaru Tsuboi, Munekazu Kanda, Tomoji Higaki, Mitsunori Matsuda
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Patent number: 6627415Abstract: The present invention provides for an isolated nucleic acid molecule encoding a human TIE-2 ligand. In addition, the invention provides for a receptorbody which specifically binds a human TIE-2 ligand. The invention also provides an antibody which specifically binds a human TIE-2 ligand. The invention further provides for an antagonist of human TIE-2. The invention further provides for a ligandbody which specifically binds TIE-2 receptor. The invention also provides for therapeutic compositions as well as a method of blocking blood vessel growth, a method of promoting neovascularization, a method of promoting the growth, differentiation or migration of cells expressing the TIE-2 receptor, including, but not limited to, hematopoietic precursor cells, a method of blocking the growth, differentiation or migration of cells expressing the TIE-2 receptor including, but not limited to, hematopoietic precursor cells, and a method of attenuating or preventing tumor growth in a human.Type: GrantFiled: November 18, 1999Date of Patent: September 30, 2003Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Samuel Davis, George D. Yancopoulos
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Patent number: 6623942Abstract: There are disclosed therapeutic compositions and methods using isolated nucleic acid molecules encoding a human myeloid progenitor inhibitory factor-1 (MPIF-1) polypeptide (previously termed MIP-3 and chemokine &bgr;8(CK&bgr;8 or ckb-8)); a human monocyte-colony inhibitory factor (M-CIF) polypeptide (previously termed MIP1-&ggr; and chemokine &bgr;1(CK&bgr;1 or ckb-1)), and a macrophage inhibitory protein-4 (MIP-4), as well as MPIF-1, M-CIF and/or MIP-4 polypeptides themselves, as are vectors, host cells and recombinant methods for producing the same.Type: GrantFiled: June 17, 1999Date of Patent: September 23, 2003Assignee: Human Genome Sciences, Inc.Inventors: Steven M. Ruben, Haodong Li
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Publication number: 20030175900Abstract: The invention concerns compositions and methods for the diagnosis and treatment of neoplastic cell growth and proliferation in mammals, including humans. The invention is based upon the identification of genes that are amplified in the genome of tumor cells. Such gene amplification is expected to be associated with the overexpression of the gene product as compared to normal cells of the same tissue type and contribute to tumorigenesis. Accordingly, the proteins encoded by the amplified genes are believed to be useful targets for the diagnosis and/or treatment (including prevention) of certain cancers, and may act as predictors of the prognosis of tumor treatment.Type: ApplicationFiled: August 2, 2002Publication date: September 18, 2003Applicant: Genentech, Inc.Inventors: Avi J. Ashkenazi, Audrey Goddard, Paul J. Godowski, Austin L. Gurney, Kenneth J. Hillan, Scot A. Marsters, James Pan, Robert M. Pitti, Margaret Ann Roy, Victoria Smith, Donna M. Stone, Colin K. Watanabe, William I. Wood
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Patent number: 6617132Abstract: Disclosed is a human CysE polypeptide and DNA (RNA) encoding such polypeptide. Also provided is a procedure for producing such polypeptide by recombinant techniques. Also disclosed are methods for utilizing such polypeptide for treating osteoporosis, tumor metastases, microbial infections, viral infection, septic shock, inflammation, retinal irritation, caries, cachicia and muscle wasting. Diagnostic methods for detecting mutations in the coding sequence and alterations in the concentration of the polypeptides in a sample derived from a host are also disclosed.Type: GrantFiled: August 29, 2001Date of Patent: September 9, 2003Assignee: Human Genome Sciences, Inc.Inventors: Jian Ni, Reiner L. Gentz, Guo-Liang Yu, Craig A. Rosen
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Patent number: 6610515Abstract: The present invention provides a medicine which promotes the production of neutrophiles in case the number of the neutrophiles decreases upon treating the tumor of a cat, and does not have side-effects, wherein said medicine is prepared by incorporating, as an active ingredient, a protein which consists of 174 amino acids, and has an activity as a feline granulocyte colony stimulating factor.Type: GrantFiled: April 24, 2000Date of Patent: August 26, 2003Assignee: Nippon Institute for Biological ScienceInventors: Akira Yamamoto, Kotaro Tuchiya, Akira Iwata, Susumu Ueda
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Patent number: 6605592Abstract: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. In particular, the present application relates to a novel human protein, Protein HOFNF53. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating diseases, disorders, and/or conditions related to these novel human secreted proteins.Type: GrantFiled: March 8, 2001Date of Patent: August 12, 2003Assignee: Human Genome Sciences, Inc.Inventors: Jian Ni, Kevin P. Baker, Charles E. Birse, Reinhard Ebner, Michele Fiscella, George A. Komatsoulis, David W. LaFleur, Paul A. Moore, Henrik S. Olsen, Craig A. Rosen, Steven M. Ruben, Daniel R. Soppet, Paul E. Young, Ping Wei, Kimberly A. Florence
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Patent number: 6602687Abstract: The present invention relates to nucleic acid sequences encoding ciliary neurotrophic factor (CNTF) and to the proteins, peptides, and derivatives produced therefrom. In various embodiments of the invention, the nucleic acid sequences, proteins, and peptides of the invention may be used in the treatment of a variety of neurological diseases and disorders, including Alzheimer's disease. In a specific embodiment of the invention, CNTF may be used to support the growth of spinal cord neurons, thereby providing a method of treating spinal cord damage caused by trauma infarction, infection, nutritional deficiency or toxic agents. The present invention also relates to a novel method for producing substantilly pure CNTF. The invention also relates to pharmaceutical compositions comprising effective amounts of CNTF gene products which may be used in the diagnosis and treatment of a variety of neurologial diseases and disorders.Type: GrantFiled: May 8, 1992Date of Patent: August 5, 2003Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Michael Sendtner, Kurt Stockli-Rippstein, Friedrich Lottspeich, Yoshihiro Arakawa, Patrick Desmond Carroll, Rudolf Georg Gotz, Georg W. Kreutzberg, Dan B. Lindholm, Piotr Masiakowski, Vivien Wong, Nancy Ip, Mark E. Furth, Nikos Panayotatos, Hans Thoenen
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Patent number: 6602688Abstract: The invention relates to the cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion molecules in E. coli. In particular, antibody fragment fusion molecules having an antibody moiety which is directed against tumors and an enzyme moiety which cleaves a nontoxic prodrug to give the toxic drug can be advantageously prepared in this way while retaining their respective functional properties.Type: GrantFiled: March 22, 1999Date of Patent: August 5, 2003Assignee: Aventis Pharma Deutschland GmbHInventors: Martin Opper, Klaus Bosslet, Joerg Czech
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Patent number: 6600020Abstract: The present invention relates to canine interleukin 18 and an interleukin 18 production method.Type: GrantFiled: December 13, 1999Date of Patent: July 29, 2003Assignee: Toray Industries, Inc.Inventor: Fumiyoshi Okano
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Patent number: 6600030Abstract: The present invention relates to a CCR2 deletion mutant, designated “CCR2-64I.” CCR2 is a C—C chemokine receptor and has been implicated as a co-receptor for HIV-1. It has been discovered that the presence of the CCR2-64I allele correlates with a postponement of AIDS outcomes, and that infected individuals who have the CCR2-64I allele are at a reduced risk for progression from HIV-1 infection to the development of clinical AIDS and death. Isolated nucleic acid molecule encoding CCR2-64I and the establishment of cell lines that express CCR2-64I provides valuable tools for continuing research on HIV infection. Diagnostic methods for analysis of the allelic frequency of CCR2 wild-type and 64I genes are provided. In addition, antibodies which bind to CCR2-64I, CCR2-64I variants, and CCR2 binding agents represent potential anti-HIV agents.Type: GrantFiled: August 10, 1998Date of Patent: July 29, 2003Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Michael Dean, Stephen J. O'Brien, Michael Smith, Mary Carrington
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Patent number: 6596853Abstract: The invention relates to the use as therapeutic agents of IL-2 peptides and derivatives having biological activity and anti-IL-2 antibodies which mimic or modulate the biological activities of IL-2. The invention also relates to DNA sequences encoding the IL-2 peptides, and to methods of using the IL-2 peptides and derivatives and anti-IL-2 antibodies to modulate or mimic or antagonize the biological activities of IL-2 in vivo and to assay for the presence and activity of the IL-2 receptor.Type: GrantFiled: September 12, 2000Date of Patent: July 22, 2003Assignee: Institut PasteurInventors: Jacques Theze, Ralph Eckenberg, Jean-Louis Moreau, Jean-Claude Mazie
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Patent number: 6596512Abstract: A nematode dopamine tansporter cDNA has been cloned, sequenced, and expressed, and provides a convenient screening assay for antiparasitics, as well as psychoactive drugs.Type: GrantFiled: July 18, 2000Date of Patent: July 22, 2003Assignees: American Cyanamid Company, Vanderbilt UniversityInventors: Randy D. Blakely, Cecil M. Eppler
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Patent number: 6586210Abstract: The present invention concerns a novel T1R-like ligand II protein. In particular, isolated nucleic acid molecules are provided encoding the T1R-like ligand II protein. T1R-like ligand II polypeptides are also provided, as are recombinant vectors and host cells for expressing the same.Type: GrantFiled: August 22, 1997Date of Patent: July 1, 2003Assignee: Human Genome Sciences, Inc.Inventors: Jian Ni, Reiner L. Gentz, Steven M. Ruben
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Patent number: 6583270Abstract: The present invention concerns a novel human cytokine. In particular, isolated nucleic acid molecules are provided encoding interleukin-19 (IL-19). IL-19 polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. The invention further concerns therapeutic methods for modulating cytokine production.Type: GrantFiled: August 31, 1999Date of Patent: June 24, 2003Assignee: Human Genome Sciences, Inc.Inventors: Craig A. Rosen, Joseph J. Kenny
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Patent number: 6566503Abstract: Nucleic acids encoding a new family of chemokines, the CX3C family, from a mammal, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided.Type: GrantFiled: January 25, 2001Date of Patent: May 20, 2003Assignee: Schering CorporationInventors: Gerard T. Hardiman, Devora L. Rossi, Kevin B Bacon, Bazan Fernando J., Thomas J. Schall, Albert Zlotnik
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Patent number: 6558672Abstract: The present invention relates to disulfide-stabilized recombinant polypeptide molecules which have the binding ability and specificity for another peptide, such as the variable region of an antibody molecule. Methods of producing these molecules and nucleic acid sequences encoding these molecules are also described. In particular, the invention discloses Fv antibody fragments stabilized by a disulfide bond connecting the VH and VL regions of the Fv fragment. The &agr; and &bgr; chains of T cell receptors may be similarly stabilized by means described in the invention.Type: GrantFiled: September 7, 2000Date of Patent: May 6, 2003Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Ira H. Pastan, Byungkook Lee, Sun-Hee Jung, Ulrich Brinkmann
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Patent number: 6558919Abstract: The invention provides ampS polypeptides and DNA (RNA) encoding ampS polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ampS polypeptides to screen for antibacterial compounds.Type: GrantFiled: December 4, 1998Date of Patent: May 6, 2003Assignee: SmithKline Beecham CorporationInventors: Magdalena Zalacain, James Raymond Brown
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Patent number: 6555346Abstract: The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×105 to 5×106 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or Em9 protein or fragments thereof in insect cell culture characterized by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 &mgr;g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, &agr;-units an/or &bgr;-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 &mgr;l.Type: GrantFiled: June 16, 2000Date of Patent: April 29, 2003Assignees: Stichting Instituut voor Dierhouderij en Diergezondheid, Bayer AGInventors: Dietmar Kretzdorn, Dirk Franciscus Marinus van de Wiel, Abraham Johannes de Smit, Robertus Jacobus Maria Moormann, Erik Kees Hamann
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Patent number: 6552169Abstract: Novel Type III density enhanced protein tyrosine phosphatases are disclosed and exemplified by human DEP-1 enzyme. Polynucleotides encoding huDEP-1 are disclosed, along with methods and materials for production of the same by recombinant procedures. Binding molecules specific for DEP-1 are also disclosed as useful for modulating the biological activities of DEP-1.Type: GrantFiled: November 23, 1999Date of Patent: April 22, 2003Assignee: Cold Spring Harbor LaboratoryInventors: Nicholas K. Tonks, Arne Ostman
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Patent number: 6551820Abstract: Plant expression vectors comprising at least two expression cassettes are provided which function to reduce transcriptional silencing of polynucleotide expression. Further, novel plant expression vectors for expression of immunogenic polypeptides, including HBsAg, are provided. The plant expression vectors can be used to produce immunogenic polypeptides, including HBsAg, in edible plant tissues. The edible plant tissues can be used to elicit an immune response in humans and animals when the plant tissues are consumed.Type: GrantFiled: December 23, 1999Date of Patent: April 22, 2003Assignee: Boyce Thompson Institute for Plant ResearchInventors: Hugh S. Mason, Yasmin Thanavala, Charles Joel Arntzen, Elizabeth Richter
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Patent number: 6551796Abstract: The present invention provides an isolated nucleic acid encoding a urate transporter, vectors comprising the nucleic acid, host cells comprising the nucleic acid, and host cells comprising the vectors. Compositions comprising the vectors and a carrier are also provided. In another embodiment, the present invention provides isolated and substantially purified urate transporter. Proteoliposomes containing substantially purified urate transporter are also provided, as well as compositions comprising the urate transporter and a carrier. The present invention further provides methods of making a urate transporter, and methods of identifying agents that inhibit or agonize the transporter.Type: GrantFiled: April 27, 2000Date of Patent: April 22, 2003Assignee: Mount Sinai School of MedicineInventors: Ruth G. Abramson, Edgar Leal-Pinto, Michael Lipkowitz
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Patent number: 6551801Abstract: A process for preparing a purified dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to e) in sequence: a) the step of treating an inclusion body of a bone-derived factor with a unfolding agent to prepare a solubilized monomer; b) the step of treating the solubilized monomer with a refolding solution to prepare a dimer; c) the step of subjecting the refolded dimer to ultrafiltration and solvent replacement; d) the step of subjecting the dimer solution prepared above to isoelectric precipitation; and e) the step of subjecting the isoelectrically precipitated dimer to reverse-phase chromatography.Type: GrantFiled: July 7, 1999Date of Patent: April 22, 2003Assignee: Biopharm Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka GmbHInventors: Hidetoshi Andou, Jun Honda, Sjunjiro Sugimoto
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Patent number: 6548272Abstract: A gene for a novel transmembrane protein was isolated and its nucleotide sequence was determined. A protein encoded by this gene (TRPC7) has 7 transmembrane regions, and because there is homology with TRP (transient receptor potential), this protein is considered to have calcium channel functions. Further, since a mutation in the TRPC7 gene is frequently observed in patients with manic-depressives insanity, TRPC7 is considered to be a pathogenic gene for bipolar affective disorder.Type: GrantFiled: July 11, 2000Date of Patent: April 15, 2003Assignee: Eiken Kagaku Kabushiki KaisyaInventors: Nobuyoshi Shimizu, Kentaro Nagamine
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Patent number: 6541220Abstract: The present invention relates to novel parathyroid hormone (PTH) and parathyroid hormone related protein (PTHrP) receptors (PTH1R and PTH3R) isolated from zebrafish. The receptors of the present invention share homology with previously identified parathyroid hormone (PTH)/parathyroid related protein (PTHrP) receptors. Isolated nucleic acid molecules are provided encoding the zebrafish PTH1R and PTH3R receptors. PTH1R and PTH3R receptor polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of PTH1R and PTH3R receptor activity and to diagnostic and therapeutic methods.Type: GrantFiled: November 30, 1999Date of Patent: April 1, 2003Assignee: The General Hospital CorporationInventors: Harald Jüppner, David A. Rubin
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Patent number: 6537780Abstract: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the enzyme peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the enzyme peptides, and methods of identifying modulators of the enzyme peptides.Type: GrantFiled: March 28, 2001Date of Patent: March 25, 2003Assignee: Applera CorporationInventors: Ellen M. Beasley, Karen A. Ketchum, Valentina Di Francesco
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Patent number: H2071Abstract: The invention provides isolated nucleic acid compounds encoding the 454 gene of Streptococcus pneumoniae. Also provided are vectors and transformed host cells for expressing the encoded protein, and a method for identifying compounds that bind and/or inhibit said protein.Type: GrantFiled: December 8, 1997Date of Patent: July 1, 2003Assignee: Eli Lilly and CompanyInventors: Robert Brown Peery, Paul Luther Skatrud, Michele Louise Young Bellido, Patti Jean Treadway