Oxygen Atom Of The Saccharide Radical Is Directly Linked Through Only Acyclic Carbon Atoms To A Nonsaccharide Heterocyclic Ring (e.g., Bleomycin, Phleomycin, Etc.) Patents (Class 435/77)
-
Patent number: 9499841Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.Type: GrantFiled: June 25, 2015Date of Patent: November 22, 2016Assignee: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Wilbert Herman Marie Heijne
-
Patent number: 9482663Abstract: The present invention refers to a method for binding a recombinant polypeptide to a carrier, wherein a layer is bound to a carrier, and the layer comprises a recombinant polypeptide on the surface distal to the carrier.Type: GrantFiled: September 9, 2010Date of Patent: November 1, 2016Assignee: Autodisplay Biotech GmbHInventors: Joachim Jose, Jae-Chul Pyun
-
Patent number: 9034602Abstract: The present invention relates to pharmaceuticals and modified beta-lactamases. Specifically, the invention relates to novel recombinant beta-lactamases and pharmaceutical compositions comprising the beta-lactamases. Also, the present invention relates to methods for modifying a beta-lactamase, producing the beta-lactamase and treating or preventing beta-lactam antibiotic induced adverse effects. Furthermore, the present invention relates to the beta-lactamase for use as a medicament and to the use of the beta-lactamase in the manufacture of a medicament for treating or preventing beta-lactam antibiotics induced adverse effects. Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.Type: GrantFiled: May 17, 2011Date of Patent: May 19, 2015Assignee: Synthetic Biologics, Inc.Inventors: Pertti Koski, Ulla Airaksinen, Katja Valimaki
-
Publication number: 20140235839Abstract: The present invention provides a novel sialo-sugar chain, a process for producing the sialo-sugar chain, and a device for producing the sialo-sugar chain. A sialo-sugar chain can be easily and efficiently mass-produced by reacting a sugar wherein a hydroxy groups is substituted with an alkynyl group (herein sometimes referred to as “alkynylated sugar”) with a specific sialic acid donor in the presence of a sialic acid-introducing enzyme.Type: ApplicationFiled: September 11, 2012Publication date: August 21, 2014Inventor: Masanori Yamaguchi
-
Patent number: 8652809Abstract: The invention relates to using a lipid acyltransferase polypeptide (SEQ ID NO: 68) in the manufacture of ultra-heat treatment milk (UHT) for improving the stability, the perceptible sensory difference, the smell, and the taste; and for reducing the cholesterol content, and for eliminating or reducing creaming of the UHT milk. The invention also relates to a method of producing UHT milk, wherein the method comprises admixing a lipid acyltransferase with milk and heating the mixture to make it a UHT milk.Type: GrantFiled: February 16, 2010Date of Patent: February 18, 2014Assignee: DuPont Nutrition Biosciences APSInventors: Niels Erik Larsen, Jørn Borch Søe
-
Patent number: 7816079Abstract: The present invention provides novel systems for sequencing nucleic acid molecules using dNTPs that are 3? end labeled with cleavable tags that block further extension and uniquely identify the bases to which they are attached. Removal of the tags liberates the 3? ends of the extension products for further extension. In related embodiments, oligonucleotides containing sequence-related cleavable tags are employed in a ligation reaction to determine the sequence of a particular DNA sample.Type: GrantFiled: June 30, 2003Date of Patent: October 19, 2010Assignee: Agilent Technologies, Inc.Inventor: Steven M. Fischer
-
Patent number: 7556948Abstract: Invented are non-peptide TPO mimetics. Also invented is a method of treating thrombocytopenia, in a mammal, including a human, in need thereof which comprises administering to such mammal an effective amount of a selected hydroxy-1-azobenzene derivative.Type: GrantFiled: August 7, 2003Date of Patent: July 7, 2009Assignee: Glaxo Group LimitedInventors: Michael John Rees, Keith Graham Robins, Anna Louisa Stefanska, Jan Edward Thirkettle, Michael Sidney Verrall, David Alan Yeandle
-
Patent number: 7060714Abstract: The invention is aimed at discovering a new type of anti-cancer compound which can exert an excellent inhibitory activity against the heparanase. A fungal strain Acremonium sp. MT70646 (KCTC 8973P) was isolated from the soil. From the culture of the isolated fungal strain, novel compounds that can inhibit both heparinase and heparanase was purified. Therefore, the present invention regards to provide a newly isolated fungal strain Acremonium sp. MT70646 (KCTC 8973P), its new products, and pharmaceutical agents such as a heparinase inhibitor, a heparanase inhibitor, a metastasis inhibitor and an angeiogenesis inhibitor all of which contain the above new compounds produced by the fungal strain as an active ingredient.Type: GrantFiled: December 19, 2000Date of Patent: June 13, 2006Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Jong Seog Ahn, Bo Yeon Kim, Won Keun Oh, Tae Ick Mheen, Dae Ook Kang, Hack Ryong Ko
-
Patent number: 6632599Abstract: The present invention relates to procedures for the detection or for the determination of solid phase-associated factors, which are multiply associated with the same solid phase. According to the invention, the sample is brought into contact with a transmitter particle, on which at least one ligand having binding affinity for a solid phase-associated factor and a transmitter are immobilized, and a receiver particle, on which at least one ligand having binding affinity for said solid phase-associated factor and a receiver is immobilized, and then the signal is determined which results when transmitter and receiver are brought sufficiently close to one another. In particular, the invention relates to the detection of cell surface receptors which can be used for the typing of cells or for the determination of cell activation states. It is thus possible to replace the hitherto widely customary flow cytometry by a more simple procedure.Type: GrantFiled: December 18, 1998Date of Patent: October 14, 2003Assignee: Dade Behring Marburg GmbHInventors: Michael Kraus, Carsten Schelp, Wilhelm Schuy
-
Patent number: 6613523Abstract: The present invention provides novel systems for sequencing nucleic acid molecules using dNTPs that are 3′ end labeled with cleavable tags that block further extension and uniquely identify the bases to which they are attached. Removal of the tags liberates the 3′ ends of the extension products for further extension. In related embodiments, oligonucleotides containing sequence-related cleavable tags are employed in a ligation reaction to determine the sequence of a particular DNA sample.Type: GrantFiled: June 29, 2001Date of Patent: September 2, 2003Assignee: Agilent Technologies, Inc.Inventor: Steven M. Fischer
-
Patent number: 6566100Abstract: The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.Type: GrantFiled: February 20, 2001Date of Patent: May 20, 2003Assignee: Eli Lilly and CompanyInventors: Richard H. Baltz, Patricia J. Solenberg, Patti J. Treadway
-
Patent number: 6562569Abstract: The present invention provides arrays having associated modified oligonucleotides that selectively bind to DNA or RNA, methods of making such arrays, assays for using such arrays, and the like. In one embodiment, the arrays of the invention exhibit an increased binding affinity with complementary nucleic acids, and in particular with complementary RNA. In another embodiment, the associated nucleic acids of the array of the invention exhibit substantial acid resistance, allowing the arrays to be treated with low pH solutions. In another embodiment, the modified associated nucleic acids of the array of the invention exhibit substantial resistance to nuclease degradation.Type: GrantFiled: March 13, 2000Date of Patent: May 13, 2003Assignee: Oligos Etc. Inc.Inventor: Roderic M. K. Dale
-
Patent number: 6531299Abstract: Cell-free systems which effect the production of polyketides employing modular polyketide synthases are described. Libraries of new and/or known polyketides may also be produced in cell-free systems employing aromatic PKS, modular PKS or both.Type: GrantFiled: June 26, 2000Date of Patent: March 11, 2003Assignees: Stanford University, Brown University Research FoundationInventors: Chaitan Khosla, Rembert Pieper, Guanglin Luo, David E. Cane
-
Publication number: 20020102562Abstract: The present invention provides recombinant polypeptides derived from CMV glycoprotein gB and truncated fragments thereof which contain at least one epitope which is immunologically identifiable with one encoded by the CMV genome. The complete characterization of the gB protein, including the identity of glycoprotein gp55, permits the production of polypeptides which are useful as standards or reagents in diagnostic tests and/or as components of vaccines. This invention provides recombinant polypeptides and recombinant polynucleotides encoding these polypeptides wherein a neutralizing epitope of gB is localized within gp55.Type: ApplicationFiled: February 20, 2001Publication date: August 1, 2002Applicant: Pasteur Merieux Serums et Vaccines S.A.Inventors: Richard Spaete, Carol Pachl
-
Patent number: 6420142Abstract: Disclosed is a method for enzymatic splitting of rutinosides, whereby rhamnose and/or corresponding glucopyranosides is/are obtained The inventive method is carried out in the presence of a solvent mixture made up of water and one or several organic solvents.Type: GrantFiled: April 25, 2001Date of Patent: July 16, 2002Assignee: Merck Patent GesellschaftInventors: Herwig Buchholz, Thomas Koppe, Michael Schleehahn
-
Patent number: 6150123Abstract: The present invention relates to a method for biotinylating a desired subset of a larger population of proteins, wherein the subset of proteins is first selectively bound to a solid phase, thereby separating it from undesired proteins of the population, and then, still bound to solid phase, it is biotinylated. Unreacted biotin may then be washed from the solid phase, and the biotinylated protein may be uncoupled from the solid phase.Type: GrantFiled: September 3, 1998Date of Patent: November 21, 2000Assignee: Centre Integre de Recherches Biocliniques sur le Sida (CIRBS)Inventor: Antonio Cosma
-
Patent number: 6143537Abstract: A method for stabilizing analyses with antibodies and antibody fragments comprises dissolving the analyte in a liquid to form a solution, adding analyte-specific antibodies, fragments of such antibodies, or both to the solution, heating the solution, and then cooling and filtering the solution. The filtered solution may be diluted in a suitable matrix.Type: GrantFiled: January 5, 1999Date of Patent: November 7, 2000Assignee: Medical Analysis Systems, Inc.Inventors: Shing F. Kwan, Ivan E. Modrovich, Rebecca J. Hunt
-
Patent number: 6132955Abstract: An electrode can be derivitized by contacting it with a derivitizing solution to make it more sensitive to a desired analyte signal as opposed to interfering signals in an assay. Particularly, in an electrochemiluminescence (ECL) immunoassay the working electrode can be derivitized to be more sensitive to desired analyte signals, as opposed to interfering non-bound conjugate or serum matrix signals.Type: GrantFiled: September 3, 1997Date of Patent: October 17, 2000Assignee: IGEN International, Inc.Inventors: David Talley, Jonathan K. Leland, Gary F. Blackburn
-
Patent number: 6132959Abstract: Disclosed and claimed is a novel inexpensive and efficient method for the early detection of HIV infection in babies. The method utilizes a unique IgA capture ELISA procedure for detection of HIV-specific antibodies.Type: GrantFiled: May 26, 1998Date of Patent: October 17, 2000Assignee: Blake Laboratories, Inc.Inventor: Milan Blake
-
Patent number: 6130313Abstract: Green fluorescent protein (GFP) is widely used as a reporter in determining gene expression and protein localization. The present invention provides fusion proteins with a half life of ten hours or less. Such proteins may be constructed by fusing C-terminal amino acids of the degradation domain of mouse ornithine decarboxylase (MODC), which contains a PEST sequence, to the C-terminal end of an enhanced variant of GFP (EGFP). Fluorescence intensity of the fusion protein in transfected cells is similar to that of EGFP, but the fusion protein, unlike EGFP, is unstable in the presence of cycloheximide. Specific mutations in the MODC region have resulted in mutants with varying half lives, useful for a variety of purposes.Type: GrantFiled: April 17, 1998Date of Patent: October 10, 2000Assignee: Clontech Laboratories, Inc.Inventors: Xianqiang Li, Steve Kain
-
Patent number: 6124105Abstract: The invention relates to a diagnostic test for the detection and identification of Mycobacterium species in biological specimens of human and animal origin. The test is based on the immunological detection of one or more antigens originating from Mycobacterium. To enable the detection of an antibody-antigen reaction, the antibodies specific for these antigens can be labelled with an enzyme or fluorescent dye or attached to latex particles or any other suitable label. The diagnostic test may be in a form of ELISA and may or may not require concentration of the Mycobacterium antigens prior to the actual test.Type: GrantFiled: February 11, 1997Date of Patent: September 26, 2000Assignee: Adock Ingram LimitedInventors: Jan Andrianus Verschoor, Sandra Noel Bye
-
Patent number: 6120856Abstract: The invention relates to an improved method for the manufacture of magnetically responsive particles, also called ferrofluids. The improved method involves a heat treatment step, which may occur at various times during the preparation of the materials, including during subdivision of the magnetic starting material, during the addion of a coating material, after formation of a magnetically responsive particle, or some combination thereof. The materials formed by such a process have numerous advantages over materials formed by other processes, including enhanced salt stability, increased coating uptake, and increased binding capacity. These ferrofluids have applications in a variety of preparative and diagnostic techniques, including immunoassay, cell separations, toxicity testing, food testing, environmental analysis, and MRI.Type: GrantFiled: October 14, 1997Date of Patent: September 19, 2000Assignee: Immunivest CorporationInventors: Paul A. Liberti, Galla C. Rao, Joseph N. Chiarappa
-
Patent number: 6103537Abstract: The present invention concerns methods for masking inhomogeneity of a member of a specific binding pair (sbp) employed in a capillary electroseparation. The method comprises binding the sbp member to synthetic particles that become localized during capillary electroseparation. Also disclosed is one embodiment of the present invention, which is a method for conducting a capillary electroseparation specific binding assay. The method involves the electroseparation of a labeled first member of a specific binding pair that is bound in a complex from labeled first member that is not bound in the complex. The complex comprises the first member and a second member of a specific binding pair. A combination is provided comprising a sample suspected of containing an analyte, a labeled first member of a specific binding pair, and a second member of a specific binding pair under conditions for forming a complex between labeled first member and the second member.Type: GrantFiled: September 30, 1998Date of Patent: August 15, 2000Assignee: ACLARA Biosciences, Inc.Inventors: Edwin F. Ullman, Gregory B. Stauber
-
Patent number: 6100041Abstract: The present invention provides polynucleotide and polypeptide molecules for a novel human prohormone convertase 4. The polynucleotides encoding human prohormone convertase 4, are located on chromosome 19, and may, for example, be used to identify a region of the genome associated with human disease states. The present invention also includes methods for producing the protein and antibodies thereto.Type: GrantFiled: August 6, 1999Date of Patent: August 8, 2000Assignee: ZymoGenetics, Inc.Inventors: Si Lok, Stephen R. Jaspers
-
Patent number: 6090610Abstract: Compound 0406 having a chemical structural formula represented by the chemical formula (1) below and having an excellent immuno suppressive activity, and pharmaceutically acceptable salts thereof. ##STR1## (wherein R.sub.1 is --COCH.sub.2 COOH or H; R.sub.2 is --CO(CH.sub.2).sub.3 CH.sub.3 or --CO(CH.sub.2).sub.4 CH.sub.3 or H.Type: GrantFiled: January 29, 1999Date of Patent: July 18, 2000Assignee: Higeta Shoyu Co., Ltd.Inventors: Yasushi Tanaka, Hisayuki Komaki, Yuzuru Mikami, Katsukiyo Yazawa, Jun'ichi Kobayashi
-
Patent number: 6083709Abstract: The invention provides a method for diagnosing in a subject a neoplastic condition which comprises (a) obtaining from the subject a sample of a biological fluid; and (b) detecting the presence in the sample of a mutant p53 polypeptide encoded by an activated p53 oncogene, the presence of the mutant p53 polypeptide in the sample indicating that the subject has the neoplastic condition.Type: GrantFiled: November 23, 1993Date of Patent: July 4, 2000Assignee: OSI Pharmaceuticals, Inc.Inventors: Frederick H. Reynolds, Jr., Ron Zeheb, John R. Stephenson, John M. Sorvillo
-
Patent number: 6046014Abstract: This invention provides new fluorescent molecules useful for detection of target entities. In particular, it relates to fluorescent adducts comprising an apoprotein and a bilin.Type: GrantFiled: August 1, 1997Date of Patent: April 4, 2000Assignee: The Regents of the University of CaliforniaInventors: John Clark Lagarias, John Thomas Murphy
-
Patent number: 6040134Abstract: The present invention relates to a method of diagnosing preclinical insulin-dependent diabetes mellitus by determining the T cell response to a specific islet cell antigen fetal antigen 1 (FA1) or the presence of autoantibodies against FA1 in serum, a test kit for use in the method, as well as a pharmaceutical composition of FA1 and a method for use of the pharmaceutical composition for therapy of insulin-dependent diabetes mellitus.Type: GrantFiled: July 15, 1997Date of Patent: March 21, 2000Assignee: Novo Nordisk A/SInventors: Ole Dragsbaek Madsen, Birgitte Koch Michelsen, Jacob Steen Petersen, Bart O. Roep, Borge Teisner
-
Patent number: 6040194Abstract: An in vivo method and apparatus for detecting an analyte in an individual. A sensor that includes a fluorescence reagent is placed in communication with the body fluids of the individual suspected of containing the analyte in such a way that once in place said sensor does not exit the skin of the individual. The sensor is configured to retain the fluorescence reagent while allowing analyte to diffuse into and out of said sensor. The sensor is illuminated transdermally, and the fluorescence from the fluorescence reagent associated with the presence of the analyte is measured.Type: GrantFiled: June 6, 1995Date of Patent: March 21, 2000Assignee: Sensor Technologies, Inc.Inventors: William L. Chick, David E. Wolf, Richard A. Cardullo
-
Patent number: 6030792Abstract: This invention provides novel antibodies and engineered versions thereof and methodology for monitoring biological media for protein fragments, especially collagen fragments resulting from collagenase cleavage of type II collagen.Type: GrantFiled: November 2, 1998Date of Patent: February 29, 2000Assignee: Pfizer IncInventors: Ivan G. Otterness, Peter S. Mezes, James T. Downs, Kimberly S. Johnson
-
Patent number: 6004995Abstract: Compound 0406 having a chemical structural formula represented by the chemical formula (1) below and having an excellent immuno suppressive activity, and pharmaceutically acceptable salts thereof. ##STR1## (wherein R.sub.1 is --COCH.sub.2 COOH or H; R.sub.2 is --CO(CH.sub.2).sub.3 CH.sub.3 or --CO(CH.sub.2).sub.4 CH.sub.3 or H.Type: GrantFiled: April 24, 1997Date of Patent: December 21, 1999Assignee: Higeta Shoyu Co., LTD.Inventors: Yasushi Tanaka, Hisayuki Komaki, Yuzuru Mikami, Katsukiyo Yazawa, Jun'ichi Kobayashi
-
Patent number: 5989804Abstract: E6-BP polypeptides, nucleic acids encoding E6-BP polypeptides, and uses thereof.Type: GrantFiled: November 14, 1995Date of Patent: November 23, 1999Assignee: New England Medical Center Hospitals, Inc.Inventors: Elliot J. Androphy, Jason J. Chen
-
Patent number: 5985589Abstract: The present invention generally relates to a novel catalytic subunit of a lipid kinase designated p110.delta.. Polynucleotides encoding p110.delta. and recombinant p110.delta. polypeptides are provided along with antibodies to p110.delta., assays for identifying inhibitors of p110.delta., and the like.Type: GrantFiled: January 6, 1999Date of Patent: November 16, 1999Assignee: ICOS CorporationInventors: David H. Chantry, Merl F. Hoekstra, Douglas A. Holtzman
-
Patent number: 5981180Abstract: A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.Type: GrantFiled: October 11, 1995Date of Patent: November 9, 1999Assignee: Luminex CorporationInventors: Van S. Chandler, R. Jerrold Fulton, Mark B. Chandler
-
Patent number: 5958713Abstract: The present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the fluorescence of (i) green fluorescent protein (GFP) having a protein kinase recognition site or (ii) GFP that has been modified to contain a second messenger binding domain.Type: GrantFiled: March 14, 1997Date of Patent: September 28, 1999Assignee: Novo Nordisk A/SInventors: Ole Thastrup, S.o slashed.ren Tullin, Lars Kongsbak Poulsen, Sara Petersen Bj.o slashed.rn
-
Patent number: 5958202Abstract: Disclosed are methods and apparatus for performing highly sensitive enzyme-amplified assays using channel electrophoresis of an enzyme having a biorecognition moiety. Combining a binding reaction of an analyte and an enzyme having a biorecognition moiety, and an electrophoretic separation which uses enzyme-amplified detection methodology, assay methods and apparatus are provided that offer simplicity, quantitative sensitivity and rapid analysis. Also disclosed are highly sensitive catalytically-amplified assays using a capillary electrophoresis format in which the binding reaction prior to electrophoresis is heterogeneous or homogeneous, and direct or competitive. The methods and apparatus for conducting sensitive automated catalytically-amplified assays using electrophoresis may be conducted in a microscale format.Type: GrantFiled: January 22, 1997Date of Patent: September 28, 1999Assignee: PerSeptive Biosystems, Inc.Inventors: Fred E. Regnier, Xian-Wei Yao, Todd A. Taylor, Martin Fuchs, Dieter Schmalzing, Lance Koutny, Wassim Nashabeh
-
Patent number: 5889164Abstract: This invention provides a novel chromanol glycoside which is a water soluble antioxidant excellent in heat and pH stability and production method thereof. It is a chromanol glycoside represented by the general formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, and R.sup.4 independently are a hydrogen atom or a lower alkyl group, R.sup.5 is a hydrogen atom, a lower alkyl group, or a lower acyl group, X is a monosaccharide residue or an oligosacoharide residue, providing the hydrogen atom of the hydroxyl group of saccharide residue may be substituted by a lower alkyl group or a lower acyl group, n is an integer in the range of 0 to 4, and m is an integer in the range of 1 to 6.Type: GrantFiled: September 27, 1995Date of Patent: March 30, 1999Assignee: CCI CorporationInventors: Hironobu Murase, Tsutomu Kunieda, Tetsuya Tsujii
-
Patent number: 5888746Abstract: A method for diagnosis or prognosis of a cancer is disclosed. The method comprises (i) detecting in a first biological sample protein tyrosine phosphate .alpha. (PTP.alpha.) or PTP.alpha. nucleic acid, and (ii) comparing the level of PTP.alpha. or PTP.alpha. nucleic acid in the first sample with the level in a second biological sample known to be from normal tissue. Any overexpression of PTP.alpha. or PTP.alpha. nucleic acid in the first sample compared to the second sample is indicative that the first sample is from a cancerous tissue.Type: GrantFiled: March 10, 1997Date of Patent: March 30, 1999Assignee: Institute of Molecular and Cell BiologyInventors: Karim Tabiti, Catherine Jane Pallen
-
Patent number: 5882910Abstract: The present invention generally relates to a novel catalytic subunit of a lipid kinase designated p110.delta.. Polynucleotides encoding p110.delta. and recombinant p110.delta. polypeptides are provided along with antibodies to p110.delta., assays for identifying inhibitors of p110.delta., and the like.Type: GrantFiled: November 25, 1997Date of Patent: March 16, 1999Assignee: ICOS CorporationInventors: David H. Chantry, Merl F. Hoekstra, Douglas A. Holtzman
-
Patent number: 5866347Abstract: A method of identifying individuals who have, or who are at risk of developing, autoimmune neuropsychiatric disorders is disclosed. The invented method relies on detection of the B lymphocyte antigen, D8/17. The invented method can conveniently be carried out as a simple blood test.Type: GrantFiled: April 8, 1997Date of Patent: February 2, 1999Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Susan E. Swedo, Henrietta L. Leonard, John B. Zabriskie
-
Patent number: 5846739Abstract: A method of determining the proliferative status of a carcinoma is disclosed. One obtains a patient sample and then quantitatively analyzes the sample for NGAL gene expression product. The amount of NGAL expression product is compared with a standard curve to determine the S-phase value. The sample can be breast tissue or breast fluid aspirate. Alternatively, blood can by analyzed for this marker to diagnose metastasis.Type: GrantFiled: January 6, 1997Date of Patent: December 8, 1998Assignee: Wisconsin Alumni Research FoundationInventors: Michael N. Gould, Steven P. Stoesz
-
Patent number: 5807758Abstract: The present invention is a method and apparatus for detecting a target species. The target molecule may be in liquid phase (in solution) or (for some embodiments of the invention) in vapor phase. A sensor according to the present invention monitors whether a target species has selectively bound to groups on the cantilever surface by monitoring the displacement of the cantilever, and hence the force acting on the cantilever. This force acting on the cantilever arises from the force acting on a structure that moves in electric or magnetic field, and that may be selectively bound to the cantilever. In the case of target species having a sufficiently large net electric charge or dipole moment, the target species itself may serve as the structure that moves in an electric field.Type: GrantFiled: July 21, 1995Date of Patent: September 15, 1998Inventors: Gil U. Lee, David A. Kidwell, Richard J. Colton
-
Patent number: 5798218Abstract: Aerolysin is shown to bind specifically to the Thy-1 antigen. Aerolysin-based methods, compositions and kits for detecting the presence of Thy-1 antigen are presented.Type: GrantFiled: September 24, 1996Date of Patent: August 25, 1998Assignee: University of British Columbia Innovation and Development CorporationInventor: James Thomas Buckley
-
Patent number: 5789179Abstract: A method of assaying for CAT in a fluid involves the use of a complex of chloramphenicol with a member of a specific binding pair such as a hapten or biotin. Biotinylated chloramphenicol is claimed as new. A scintillation proximity assay involves use of this reagent with tritiated acetyl coenzyme A and streptavidin coated SPA beads.Type: GrantFiled: May 22, 1997Date of Patent: August 4, 1998Assignee: Amersham International plcInventors: Gerard Philip Brophy, William Jonathan Cummins, Christopher Robert Mundy
-
Patent number: 5786460Abstract: Novel antifungal antibiotics Cepacidine A (A.sub.1 and A.sub.2 ),a novel microorganism Pseudomonas cepacia AF 2001 producing the same, and a process for producing the said antibiotics are disclosed.Type: GrantFiled: June 18, 1996Date of Patent: July 28, 1998Assignee: Cheil Foods & Chemicals, Inc.Inventors: Sung Ho Kim, Bong Chul Hyun, Jung Woo Suh, Chang One Kim, Yoong Ho Lim, Chul Hoon Lee
-
Patent number: 5726025Abstract: The present invention provides a systematic and practical approach for the identification of candidate agents able to inhibit ubiquitin-mediated degradation of a cell-cycle regulatory protein, such as cyclins. One aspect of the present invention relates to a method for identifying an inhibitor of ubiquitin-mediated proteolysis of a cell-cycle regulatory protein by (i) providing a ubiquitin-conjugating system that includes the regulatory protein and ubiquitin under conditions which promote the ubiquitination of the target protein, and (ii) measuring the level of ubiquitination of the subject protein brought about by the system in the presence and absence of a candidate agent. A decrease in the level of ubiquitin conjugation is indicative of an inhibitory activity for the candidate agent.Type: GrantFiled: April 20, 1995Date of Patent: March 10, 1998Assignee: President and Fellows of Harvard CollegeInventors: Marc W. Kirschner, Randall W. King, Jan-Michael Peters
-
Patent number: 5716788Abstract: One shortcoming of methotrexate chemotherapy is that previously responsive tumors can become refractory to methotrexate after continued exposure. Such methotrexate resistance may be due to underexpression of reduced folate carrier (RFC) protein. The present invention provides DNA molecules encoding human RFC. The present invention also relates to expression vectors comprising RFC-encoding DNA molecules, and to the use of such vectors to restore methotrexate sensitivity in mammalian cells. The present invention further relates to antibodies that bind with human RFC protein, and to methods of detecting human RFC protein using such antibodies.Type: GrantFiled: June 7, 1995Date of Patent: February 10, 1998Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Jeffrey A. Moscow, Kenneth H. Cowan, Kathy Dixon, Rui He
-
Patent number: 5710248Abstract: This invention discloses the incorporation of a peptide QRQYGDVFKGD (SEQ ID NO:1) from glycoprotein gE of Varicella zoster virus into a protein or polypeptide sequence for immunoisolation, immunopurification and immunodetection.Type: GrantFiled: July 29, 1996Date of Patent: January 20, 1998Assignee: University of Iowa Research FoundationInventor: Charles F. Grose
-
Patent number: 5698271Abstract: The invention relates to an improved method for the manufacture of magnetically responsive particles, also called ferrofluids. The improved method involves a heat treatment step, which may occur at various times during the preparation of the materials, including during subdivision of the magnetic starting material, during the addion of a coating material, after formation of a magnetically responsive particle, or some combination thereof. The materials formed by such a process have numerous advantages over materials formed by other processes, including enhanced salt stability, increased coating uptake, and increased binding capacity. These ferrofluids have applications in a variety of preparative and diagnostic techniques, including immunoassay, cell separations, toxicity testing, food testing, environmental analysis, and MRI.Type: GrantFiled: June 7, 1995Date of Patent: December 16, 1997Assignee: Immunivest CorporationInventors: Paul A. Liberti, Galla C. Rao, Joseph N. Chiarappa
-
Patent number: 5677133Abstract: A method is described for performing an affinity assay comprising contacting a sample to be assayed for the presence of an analyte with a dry reagent containing the analyte (hapten, antigen, antibody, receptor, or complementary polynucleotide) bound to a reaction cascade initiator, an antibody or other binding pair partner reactive with said analyte, and magnetic particles, to form an assay mixture in a reaction chamber, incubating the assay mixture, applying an oscillating or moving static magnetic field to the assay mixture, activating the reaction cascade initiator to initiate a reaction cascade, monitoring the response of the magnetic particles to the oscillating or moving static magnetic field to provide a time varying signal, and determining the analyte concentration of the sample by analysis of the time varying signal, as well as a kit for performing the assay and a diagnostic system for performing the assay.Type: GrantFiled: September 11, 1996Date of Patent: October 14, 1997Assignee: Cardiovascular Diagnostics, Inc.Inventor: Bruce J. Oberhardt