Lactobacillus Casei Patents (Class 435/856)
  • Patent number: 8980611
    Abstract: Compositions and methods useful for reducing or eliminating the presence of pathogens in meat or meat products are disclosed. Administration of one or more lactic acid producing microorganisms to a live animal, to a carcass, to meat, to meat products, or in animal feed results in significant reductions in the amount of pathogens potentially harmful to humans when ingested. Synergistic effects can be achieved with the administration of multiple strains of microorganisms.
    Type: Grant
    Filed: July 25, 2013
    Date of Patent: March 17, 2015
    Assignee: Guardian Food Technologies, LLC
    Inventors: Douglas R. Ware, Mindy M. Brashears
  • Patent number: 8889119
    Abstract: This disclosure relates to enhancing growth and/or activity of lactobacilli using a prebiotic formulation which includes iso-malto oligosaccharides and ?-galactosidase; and to enhancing growth and/or activity of bifidobacteria using a prebiotic formulation which includes iso-malto oligosaccharides and ?-glucanase. Other combinations of fibers and enzymes are described below which also stimulate growth and activity of lactobacilli or bifidobacteria. These combinations of enzymes and prebiotics can be taken separately or added to foods, including desserts.
    Type: Grant
    Filed: September 7, 2013
    Date of Patent: November 18, 2014
    Assignee: Master Supplements, Inc.
    Inventors: Randolph S. Porubcan, Sonja L. Yonak
  • Patent number: 8846082
    Abstract: An encapsulation system is provided comprising nitrogen-purge, instant bonding encapsulation method. Specifically, the encapsulation system comprises a composition, a two-piece capsule comprising a capsule cap and a capsule body; a gas to purge oxygen from the composition within the capsule; and a sealing solution to seal the capsule cap to the capsule body. Associated methods for encapsulating compositions using the encapsulation system are also provided.
    Type: Grant
    Filed: March 21, 2006
    Date of Patent: September 30, 2014
    Assignee: Vita-Herb Nutriceuticals, Inc.
    Inventor: Bing Baksh
  • Patent number: 8758742
    Abstract: An encapsulation system is provided comprising nitrogen-purge, instant bonding encapsulation method. Specifically, the encapsulation system comprises a composition, a two-piece capsule comprising a capsule cap and a capsule body; a gas to purge oxygen from the composition within the capsule; and a sealing solution to seal the capsule cap to the capsule body. Associated methods for encapsulating compositions using the encapsulation system are also provided.
    Type: Grant
    Filed: April 11, 2007
    Date of Patent: June 24, 2014
    Assignee: Probiohealth, LLC
    Inventor: A. Satyanarayan Naidu
  • Patent number: 8722035
    Abstract: Disclosed are formulations for enhancing the in vivo colonization of probiotic microorganisms that include digestive enzymes and probiotic microorganisms, and polysorbate surfactants. The enzymes include lactogenic enzyme formulations that promote growth of Lactobacillus probiotics, bifidogenic enzyme formulations that promote growth of Bifidobacterium probiotics and combination formulations that benefit both types of probiotics. It has been discovered that certain polysorbate surfactants, including polysorbate-60 and polysorbate-80, further promote probiotic microorganism growth, when used with the enzyme formulations. The formulations are preferably compounded as dry powders, to avoid water reaction with the enzymes in blended formulations. Such formulations can be contained in capsules, tablets, packets or bottles and administered orally, either sequentially or in one combined formulation.
    Type: Grant
    Filed: October 6, 2011
    Date of Patent: May 13, 2014
    Assignee: Master Supplements, Inc.
    Inventor: Randolph S. Porubcan
  • Patent number: 8329190
    Abstract: The invention relates to a composition for modulating the immune responses induced by Gram negative bacteria, potential pathogenic Gram positive bacteria and/or their derivatives, comprising lipoteichoic acid from lactic acid bacteria as an active ingredient. It also relates to the use of a lipoteichoic acid from lactic acid bacteria as an active ingredient and/or lactic acid bacteria producing it and/or its supernatant of culture, in the manufacture of a medicament, an oral or topical product for cosmetic, dermatological or ophtalmological applications, a food or petfood composition for modulating bacterial colonization, immune responses and decreasing the inflammatory processes associated with bacterially-mediated disease and infection in the gastrointestinal tract, bone, skin, eye, ear, lung and oral cavity. The invention also relates to lipoteichoic acid selected thereof.
    Type: Grant
    Filed: October 7, 2008
    Date of Patent: December 11, 2012
    Assignees: Societe des Produits Nestle S.A., Nestec S.A.
    Inventors: Karine Vidal, Anne Donnet-Hughes, Dominique-Anne Granato, Irene Corthesy-Theulaz
  • Patent number: 8071088
    Abstract: Compositions and methods useful for reducing or eliminating the presence of pathogens in meat and meat products are disclosed. Administration of one or more lactic acid producing microorganisms to a live animal, to a carcass, to meat, to meat products, or in animal feed results in significant reductions in the amount of pathogens potentially harmful to humans when ingested. Synergistic effects can be achieved with the administration of multiple strains of microorganisms.
    Type: Grant
    Filed: August 22, 2007
    Date of Patent: December 6, 2011
    Assignee: Guardian Food Technologies, LLC
    Inventors: Bryan E. Garner, Douglas R. Ware
  • Patent number: 8066986
    Abstract: Disclosed are formulations for enhancing the in vivo colonization of probiotic microorganisms that include digestive enzymes and probiotic microorganisms, and polysorbate surfactants. The enzymes include lactogenic enzyme formulations that promote growth of Lactobacillus probiotics, bifidogenic enzyme formulations that promote growth of Bifidobacterium probiotics and combination formulations that benefit both types of probiotics. It has been discovered that certain polysorbate surfactants, including polysorbate-60 and polysorbate-80, further promote probiotic microorganism growth, when used with the enzyme formulations. The formulations are preferably compounded as dry powders, to avoid water reaction with the enzymes in blended formulations. Such formulations can be contained in capsules, tablets, packets or bottles and administered orally, either sequentially or in one combined formulation.
    Type: Grant
    Filed: January 30, 2008
    Date of Patent: November 29, 2011
    Assignee: Master Supplements, Inc.
    Inventor: Randolph S. Porubcan
  • Patent number: 7270994
    Abstract: The invention disclosed a kind of lactobacillus casei Bd-II, the accession number of the deposit of which is CGMCC NO.0849, and also disclosed a use of it for reducing blood lipid level. Further, a composition for reducing blood lipid level containing it and an acceptable carrier is disclosed. The carrier can be skimmed milk.
    Type: Grant
    Filed: August 4, 2003
    Date of Patent: September 18, 2007
    Assignee: Shanghai Bright Dairy & Food Co. Ltd.
    Inventors: Nengqun Jiang, Benheng Guo, Liguo Sun
  • Patent number: 7214370
    Abstract: Probiotic compositions derived from Lactobacillus casei ATCC number PTA 3945 are disclosed. More specifically, probiotic compositions containing prebiotic compositions including edible oils are provided. The probiotic/prebiotic compositions disclosed herein are packaged using a nitrogen purge instant bonding anaerobic encapsulation process to maintain high viability for extended periods.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: May 8, 2007
    Assignee: ProbioHealth, LLC
    Inventor: A. Satyanarayan Naidu
  • Patent number: 7112322
    Abstract: The present invention is in the field of prophylaxis of allergies, and relates specifically to primary prevention of atopic diseases by administering probiotic bacteria, beneficial microbes of the healthy gut flora, pre- and postnatally to children at high risk of atopic diseases. In an embodiment, probiotic bacteria are administered to a pregnant woman at least two weeks before infant delivery and after delivery, probiotic bacteria are administered to the newborn infant for at least two months. The amount of probiotic bacteria administered is about 1×108 to about 1×1010 colony forming units of the probiotic bacteria.
    Type: Grant
    Filed: June 20, 2001
    Date of Patent: September 26, 2006
    Inventors: Erika Isolauri, Seppo Salminen
  • Patent number: 7052688
    Abstract: The disclosure is of a combination of lactic acid bacteria comprising: (a) a first component consisting of at least one strain of H2O2-producing lactic acid bacteria; and (b) a second component consisting of at least one strain of arginine-utilizing lactic acid bacteria. The disclosure is also of the use of this combination for making a food supplement, a hygiene product or a pharmaceutical preparation for the prevention and/or treatment of infections and inflammatory conditions caused by bacteria, viruses or fungi, especially in the mouth, vagina, urethra, nose, eyes and ears.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: May 30, 2006
    Assignee: VSL Pharmaceuticals, Inc.
    Inventor: Claudio De Simone
  • Patent number: 6797266
    Abstract: Probiotic compositions are provided that comprise Lactobacillus casei strain KE01 having ATCC accession number PTA-3945. The disclosed probiotic compositions are useful in inhibiting enteric pathogen diseases in animals and in maintaining animal health. Methods of making and using Lactobacillus casei strain KE01 probiotic compositions are also disclosed as are methods of using these probiotic compositions.
    Type: Grant
    Filed: December 17, 2001
    Date of Patent: September 28, 2004
    Assignee: Probiohealth
    Inventor: A. Satyanarayan Naidu
  • Patent number: 6664100
    Abstract: A method for converting organic solid waste into humic products and the corresponding apparatus, in which a preliminary aerobic fermentation of the organic solid waste is performed, followed by a second aerobic fermentation of the organic waste in the presence of specific microorganisms in order to provide a basic precursor on which the selective metabolization occurs of specific bacterial strains to provide the humic product.
    Type: Grant
    Filed: February 25, 1999
    Date of Patent: December 16, 2003
    Inventor: Riccardo Reverso
  • Patent number: 6589771
    Abstract: A method for arousing dormant bacteria. The method comprises inducing diffusion of intracellular solutes from dormant bacteria and then allowing an adjustment period for a length of time sufficient to initiate arousal. The decrease in intracellular osmolality or pH can be induced by methods such as extraction, dilution, or dialysis. The method has been standardized using Dulbecco's phosphate buffered saline as the solution. The aroused bacteria can then be selected or recovered by growing them on media for a period of time. If the adjustment period is prolonged, many bacteria can become hypermutative.
    Type: Grant
    Filed: October 28, 1999
    Date of Patent: July 8, 2003
    Assignee: Immunom Technologies, Inc.
    Inventor: William E. Marshall
  • Patent number: 6582695
    Abstract: The use of sphingomyelinase to increase the levels of skin and mucosal ceramides, as well as dermatological and cosmetic compositions containing same which are suitable for topical application are disclosed.
    Type: Grant
    Filed: May 22, 2001
    Date of Patent: June 24, 2003
    Assignee: VSL Pharmaceuticals Inc.
    Inventors: Renata Maria Anna Cavaliere Vesely, Claudio De Simone
  • Patent number: 6410305
    Abstract: A process and composition for treating an animal waste in a waste holding facility to reduce sulfides and enhance efficient degradation of large amounts of organic matter with reduced odor. The process includes administering a probiotic material capable of promoting organic digestion to an animal and maintaining a sulfide gas concentration of less than 10 ppm from a waste produced by the animal. Maintaining a low sulfide gas concentration can be done by adding an innoculum of sulfide-utilizing bacteria to the waste produced by the animal.
    Type: Grant
    Filed: September 17, 1999
    Date of Patent: June 25, 2002
    Assignee: BioSun Systems Corporation
    Inventors: Guy W. Miller, Gregory Scott Patterson
  • Patent number: 6340585
    Abstract: The present invention relates to a novel defined medium suitable to cultivate lactic acid bacteria, such as Lactobaccilus or Bifidobacteria. In particular the present invention pertains to the use of said medium for the isolation of bioactive molecules or functional metabolites.
    Type: Grant
    Filed: March 22, 2000
    Date of Patent: January 22, 2002
    Assignee: Nestec S.A.
    Inventors: Marina Elli, Ralf Zink, Barbara Marchesini-Huber, Roberto Reniero
  • Patent number: 6214336
    Abstract: The invention refers to the use of a strain of Lactobacillus for the manufacture of a medicament for reduction of the fibrinogen level, and optionally cholesterol level, in blood. Said medicament can be utilized for the prophylaxis and/or treatment of circulatory diseases.
    Type: Grant
    Filed: February 7, 2000
    Date of Patent: April 10, 2001
    Assignee: Probi AB
    Inventors: Hanna Bukowska, Marie-Louise Johansson, Marek Naruszewicz
  • Patent number: 6051552
    Abstract: The present invention is directed towards isolated lactobacillus biosurfactants and the process for producing same. The present invention is also directed to methods for preventing urogenital infection in mammals using the isolated lactobacillus biosurfactant. The present invention is further directed to methods of inhibiting microbial biofilm formation using the isolated lactobacillus biosurfactant to prevent the formation of bacterial biofilms, and to displace adherent biofilm-forming bacteria from surfaces.
    Type: Grant
    Filed: May 30, 1997
    Date of Patent: April 18, 2000
    Assignee: Urex Biotech, Inc.
    Inventors: Gregor Reid, Andrew W. Bruce, Henk J. Busscher, Henny C. Van der Mei
  • Patent number: 6022537
    Abstract: Bacterial strains of genus Bacillus closely phenotypically related to genus Lactobacillus. The strains are moderately thermophilic, have amylolytic properties and/or are capable of producing L(+) lactate, and are useful for producing metabolites such as L(+) lactate.
    Type: Grant
    Filed: January 10, 1996
    Date of Patent: February 8, 2000
    Assignee: Institut Francais de Recherche Scientifique pour le Developpement en Cooperation (Orstom)
    Inventors: Yannick Combet-Blanc, Bernard Ollivier, Jean-Louis Garcia
  • Patent number: 5952207
    Abstract: A process of treating whey produced as a by-product of cheese manufacture is disclosed. The process eliminates the contaminating effects of proteins including albumin and globulins within the whey. The fermentation of the treated whey produces enantromerically enriched lactic acid.
    Type: Grant
    Filed: October 7, 1997
    Date of Patent: September 14, 1999
    Inventors: Juan Fuertes Gonzalez, Angela Maria Moya Mora
  • Patent number: 5804179
    Abstract: This invention relates to lactobacillus compositions and methods of employing said compositions for treating or preventing urinary tract infections. More particularly, this invention relates to the ability of certain strains of lactobacilli to adhere to uroepithelial or vaginal epithelial cells and to exhibit inhibitory activity against the growth of pathogenic bacteria.
    Type: Grant
    Filed: September 30, 1994
    Date of Patent: September 8, 1998
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Andrew W. Bruce, Gregor Reid
  • Patent number: 5759541
    Abstract: Novel polypeptides having fibrinogenolytic properties and having amino acid sequences as SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6 indicated in the Sequence Listing, the preparation and use thereof for controlling diseases.
    Type: Grant
    Filed: July 25, 1996
    Date of Patent: June 2, 1998
    Assignee: BASF Aktiengesellschaft
    Inventors: Alfred Bach, Heinz Hillen, Siegfried Bialojan
  • Patent number: 5645830
    Abstract: This invention relates to Lactobacillus, skim milk, Lactobacillus Growth Factor (LGF) and Lactobacillus compositions and methods of employing the compositions for preventing urogenital infections. More particularly, this invention relates to the ability of strains of hydrophobic or hydrophilic Lactobacillus to adhere to biomaterials, and intestinal, vaginal and uroepithelial cells, to resist the action of certain antimicrobial agents and to dominate the urogenital flora.
    Type: Grant
    Filed: September 19, 1994
    Date of Patent: July 8, 1997
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Gregor Reid, Andrew W. Bruce
  • Patent number: 5506123
    Abstract: A method of processing waste is disclosed wherein the municipal solid waste is segregated and processed to recover reusable rubber, metal, plastic, glass and the remaining organic portion of the waste stream is used to make lactic acid and other chemicals. One process utilizes a pretreatment step with dilute sulfuric acid to reduce the heavy metal content of the cellulosic component of the municipal solid waste which may contaminate the produced lactic acid or inhibit the fermentation of the sugars obtained from such waste. In another, the heavy metal content of the cellulosic component of municipal solid waste is removed via an ionic exchange process, after hydrolysis with sulfuric acid. A process for an economical, energy efficient production of lactic acid from municipal solid waste is also disclosed.
    Type: Grant
    Filed: April 14, 1995
    Date of Patent: April 9, 1996
    Assignee: Controlled Environmental Systems Corporation
    Inventors: Rodger Chieffalo, George R. Lightsey
  • Patent number: 5474932
    Abstract: A process for isolation of a strain of Lactobacillus having the ability of being established on human intestinal mucosa in vivo and being able to remain therein after oral administration for at least 10 days after the completion of the administration.By the process the new strains L. plantarum 299 (DSM 6595) and L. casei ssp. rhamnosus 271 (DSM 6594) have been isolated, which are useful for the prophylaxis or treatment of bacterial infections, especially in the form of a fermented nutrient composition.
    Type: Grant
    Filed: April 16, 1993
    Date of Patent: December 12, 1995
    Assignee: Probi AB
    Inventors: Stig Bengmark, Siv Ahrne, Goran Molin, Bengt Jeppson
  • Patent number: 5378458
    Abstract: The invention relates to a novel microorganism strain Lactobacillus casei ssp. rhamnosus LC-705, DSM 7061, having a yeast and mold controlling effect, to bacterial preparations comprising this strain, alone or in combination with a bacterium of the genus Propionibacterium or another strain of the bacterium Lactobacillus casei and/or with conventional agents used for yeast and mold control. The invention also relates to the use of said strain and said bacterial preparations for yeast and mold control and to a method for controlling the growth of yeasts and molds using a bacterial preparation comprising said strain.
    Type: Grant
    Filed: September 30, 1993
    Date of Patent: January 3, 1995
    Assignee: Valio Meijerien Keskusosuusliike, Valio Ltd.
    Inventors: Annika Mayra-Makinen, Tarja Suomalainen
  • Patent number: 5354687
    Abstract: Described herein is a bacterium having high antimutagenicity against mutagens and belonging to the genus of Lactobacillus. The mutagens may comprise both a base-pair change mutagen and a frameshift mutagen. The bacterium preferably has high intestine reachability and can be Lactobacillus casei.
    Type: Grant
    Filed: March 1, 1993
    Date of Patent: October 11, 1994
    Assignee: Japanese Research & Development Association For New Food Materials
    Inventors: Hideo Hashimoto, Hayami Ito
  • Patent number: 5264347
    Abstract: The selective determination of .alpha.-ketoisocaproate in samples of body fluids, especially in serum, is achieved, without previous separation, directly in the fluid and in the presence of NADH, by means of a KIC-specific D-.alpha.-hydroxy-isocaproic dehydrogenase that originates specifically from strains of Lactobacillus casei producing this enzyme, in particular strains of Lactobacillus casei subspecies pseudoplantarum or casei. The KIC determination is used, in particular, for diagnosing maple syrup urine disease. Expedient for this purpose are analytical kits which have a divisible stock of D-.alpha.-hydroxyisocaproic dehydrogenase from Lactobacillus casei, in particular from the Lactobacillus casei (DSM 20008).
    Type: Grant
    Filed: August 5, 1992
    Date of Patent: November 23, 1993
    Assignee: Kernforschungsanlage Juelich GmbH
    Inventors: Werner Hummel, Udo Wendel, Peter Schadewaldt
  • Patent number: 5256552
    Abstract: 2-Oxo-4-phenylbutyric acid is treated with a microorganism, which has been optionally treated, capable of asymmetrically reducing 2-oxo-4-phenylbutyric acid into either (R)-2-hydroxy-4-phenylbutyric acid or (S)-2-hydroxy-4-phenylbutyric acid, and the (R)-2-hydroxy-4-phenylbutyric acid or (S)-2-hydroxy-4-phenylbutyric acid thus produced is recovered to thereby give optically active 2-hydroxy-4-phenylbutyric acid.The optically active 2-hydroxy-4-phenylbutyric acid is an important intermediate in the synthesis of various drugs such as a remedy for hypertension.
    Type: Grant
    Filed: July 17, 1992
    Date of Patent: October 26, 1993
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Teruyuki Nikaido, Yoshinori Kobayashi
  • Patent number: 5236833
    Abstract: A fermented pectin-based substance containing diacetyl is prepared by fermenting an aqueous pectin slurry with a lactic acid-producing bacteria under aerobic conditions. Prior to fermentation, the pectin may be incubated with a pectinase.
    Type: Grant
    Filed: March 17, 1992
    Date of Patent: August 17, 1993
    Assignee: Nestec S.A.
    Inventors: Shirley A. Duboff, Steven S. Kwon, Dharam V. Vadehra
  • Patent number: 5204247
    Abstract: A new strain Lactobacillus sp. KPB-176, which does not possess strict selectivity for specific media and which involves no reduction in the productivity of polysaccharides even during subculture, was isolated from kefir grains. When this new strain is cultured on a medium containing milk whey and casamino acid or when on a medium containing carbohydrate and yeast extract, capsular polysaccharides are produced in high yields.
    Type: Grant
    Filed: October 30, 1991
    Date of Patent: April 20, 1993
    Assignee: Sumitomo Heavy Industries, Ltd.
    Inventors: Susumu Adachi, Takahiro Toba, Takao Mukai, Takashi Watanabe, Haruhiko Yokoi
  • Patent number: 5164314
    Abstract: A process for the enzymatic preparation of acetoin, particularly (+)-acetoin, employs a diacetyl reductase which can be obtained from a yeast or Lactobacillus strain.
    Type: Grant
    Filed: June 18, 1991
    Date of Patent: November 17, 1992
    Assignee: Forschungszentrum Juelich GmbH
    Inventors: Werner Hummel, Maria R. Kula, Frank Boermann
  • Patent number: 5084565
    Abstract: Nucleic acid probes capable of specifically hybridizing to rRNA of E. coli and Shigella species and not to rRNA of non-E. coli/Shigella are described along with methods utilizing such probes for the specific detection of E. coli and/or Shigella in food and other samples.
    Type: Grant
    Filed: August 18, 1988
    Date of Patent: January 28, 1992
    Assignee: Gene-Trak Systems
    Inventors: Kyriaki Parodos, Hsien-Yeh Hsu, David Sobell, Janice M. McCarty, David J. Lane
  • Patent number: 5002881
    Abstract: The present invention relates to a process for the fermentative preparation of lactic acid, by allowing a sterilized growth medium to be continuously fermented in a reaction vessel by a culture of bacilli of the genus Bacillus, which form lactic acid, keeping the pH of the reaction mixture at 3 to 9 with an aqueous solution of XOH (where X is NH.sub.4 or a metal whose hydroxide and whose salt of lactic acid are watersoluble), subjecting the fermented reaction mixture to an ultrafiltration, recycling the retentate to the reaction vessel, and concentrating the permeate and subsequently subjecting it to an electrodialysis wherein bipolar membranes are used, in which process the lactate is decomposed into the lactic acid and XOH.
    Type: Grant
    Filed: June 13, 1989
    Date of Patent: March 26, 1991
    Assignee: Cooperatieve Vereniging Suiker Unie U.A.
    Inventors: Joannes G. M. Van Nispen, Ronald Jonker
  • Patent number: 5001058
    Abstract: A new antibiotic having the chromophore structure of the plurmycin family of antibiotics is produced by fermentation of Streptomyces violaceus ATCC 53807. The new antibiotic designated BU-3839T, exhibits potent antibacterial activity and also inhibits the growth of tumors in experimental animal systems.
    Type: Grant
    Filed: November 17, 1989
    Date of Patent: March 19, 1991
    Assignee: Bristol-Myers Company
    Inventors: Masataka Konishi, Keiko Shimizu, Masaru Ohbayashi, Koji Tomita, Takeo Miyaki, Toshikazu Oki
  • Patent number: 4956177
    Abstract: A method for providing fungal inhibition using Lactobacillus casei var. rhamnosus which produces an antifungal substance is described. Bacterial compositions of LCR are also described. The method and compositions are particularly useful for producing fermented foods.
    Type: Grant
    Filed: November 4, 1985
    Date of Patent: September 11, 1990
    Assignee: Microlife Technics, Inc.
    Inventors: Stephen W. King, Geoff G. Fowler, Peter A. Vandenbergh
  • Patent number: 4912047
    Abstract: An improved method for inhibiting psychrotrophic bacteria in raw milk is described. The method uses a non-lactose fermenting strain of a lactobacillus to generate hydrogen peroxide which inhibits the psychrotrophic bacteria. A preferred strain is Lactobacillus casei subspecies alactosus NRRL-B-12344 which is a superior producer of hydrogen peroxide at 5.degree. to 8.degree. C.
    Type: Grant
    Filed: May 15, 1987
    Date of Patent: March 27, 1990
    Assignee: Microlife Technics, Inc.
    Inventors: Mark A. Matrozza, Marianne F. Leverone, Donald P. Boudreaux
  • Patent number: 4771001
    Abstract: The disclosure describes a method for producing lactic acid from carbohydrate-containing media by continuous fermentation utilizing particular methods of media pretreatment, cell-recycle fermentation, fermentation broth acidification, and lactic acid separation.
    Type: Grant
    Filed: September 24, 1986
    Date of Patent: September 13, 1988
    Assignee: Neurex Corp.
    Inventors: Richard B. Bailey, Dilip K. Joshi, Stephen L. Michaels, Richard A. Wisdom
  • Patent number: 4698303
    Abstract: The invention provides a method for producing lactic acid by continuous fermentation utilizing particular methods of media pretreatment, cell-recycle fermentation, fermentation broth acidification, and lactic acid separation.
    Type: Grant
    Filed: March 27, 1986
    Date of Patent: October 6, 1987
    Assignee: Engenics, Inc.
    Inventors: Richard B. Bailey, Dilip K. Joshi, Stephen L. Michaels, Richard A. Wisdom
  • Patent number: 4666497
    Abstract: Cultivation of a microorganism in a nutrient medium including disrupted sea kelp or a sea kelp extract produces a bioactivating fermentation product which promotes growth and yields when applied to plants. A composition containing such bioactivating fermentation product and an acidification extraction product of humic acid is readily absorbed by plant foliage when sprayed on plants.
    Type: Grant
    Filed: October 31, 1985
    Date of Patent: May 19, 1987
    Assignee: Bio-Organics, Inc.
    Inventor: Abraham I. Tenzer
  • Patent number: 4609623
    Abstract: The enzyme D-2-hydroxy-4-methylpentanoic acid dehydrogenase has been prepared by culturing readily available Lactobacillus or Leuconostoc, microorganisms, such as Lactobacillus casei ssp. pseudoplantarum and Leuconostoc mesenteroides. The microbiologically produced enzyme has special characteristics and is capable of converting D-2-hydroxycarboxylic acids, such as D-2-hydroxy-4-methylpentanoic acid to the corresponding 2-ketocarboxylic acid and is capable of enzymatically converting 2-ketocarboxylic acids, such as 2-keto-4-methylpentanoic acid and 2-ketobutyric acid to the corresponding D-2-hydroxycarboxylic acid.
    Type: Grant
    Filed: June 6, 1984
    Date of Patent: September 2, 1986
    Assignees: Degussa AG, Society for Biotechnical Research
    Inventors: Wolfgang Leuchtenberger, Werner Hummel, Maria-Regina Kula, Horst Schutte
  • Patent number: 4579739
    Abstract: A must is fermented with a combination of at least one yeast and at least one lactobacillus, the former being selected from the group of Saccharomyces cerevisiae and Kluyveromyces lactis and the latter being selected from the group of Lactobacillus casei and Lactobacillus hilgardii for their symbiotic ability and capability to produce a synergistic organoleptic effect which eliminates all after-taste of yeast. The must is inoculated such that the respective numbers of yeast germs and lactobacilli germs per ml have a ratio of from 1:10 to 1:500.
    Type: Grant
    Filed: December 13, 1983
    Date of Patent: April 1, 1986
    Assignee: Nestec S. A.
    Inventors: John Darbyshire, Jaroslav Dasek, Hugh Hose
  • Patent number: 4579740
    Abstract: A method is described for producing fermented foods by generating lactic acid in the food using a culture having the rapid, low temperature fermentation characteristics of Lactobacillus casei NRRL-B-15,438 and a stimulatory food grade metal salt, wherein the culture has unique rapid low temperature fermentation characteristics and wherein starch is not fermented by the culture. In order to provide rapid fermentation, the stimulatory, food grade metal salt, usually a manganese salt, is provided in the food or the culture which is added to the food with the selected lactobacillus to accelerate fermentation. The cultures are particularly suited for the controlled fermentation of carbohydrates, naturally present in or added to the food to provide a selected final pH.
    Type: Grant
    Filed: July 11, 1983
    Date of Patent: April 1, 1986
    Assignee: Microlife Technics, Inc.
    Inventor: Mark A. Matrozza
  • Patent number: 4528269
    Abstract: An improved method which differentiates or separates heterogeneous populations of fast and slow acid producing strains of bacteria by growth of the strains under closely controlled unique conditions so as to allow the selection of a colony of one or the other strains is described. Preferably a gelled, solid growth medium containing in admixture: (1) milk protein, a milk protein derivative, or a milk protein substitute; (2) an acid pH sensitive color change indicator; and, (3) a buffering agent is used. The colonies have a contrasting color within and around them because of the effect of the acid produced by the bacteria on the indicator. The growth of the bacteria is under anaerobic or near anaerobic conditions in order to achieve certainty in the colony selection for fast or slow acid production. The bacteria can also be mixed with phage which inhibit or kill the members of a heterogeneous or homogeneous population of bacteria on the medium and grown to produce phage resistant colonies.
    Type: Grant
    Filed: November 29, 1982
    Date of Patent: July 9, 1985
    Assignee: The State of Oregon by and through the Oregon State Board of Higher Education on behalf of Oregon State University
    Inventors: William E. Sandine, Alan R. Huggins
  • Patent number: 4514424
    Abstract: A method is described for producing fermented foods by generating lactic acid in the food using a culture of a lactobacillus similar to Lactobacillus casei subspecies alactosus NRRL-B-12,344 and a stimulatory food grade metal salt, wherein the culture has unique rapid low temperature fermentation characteristics and wherein lactose, glycogen, and starch are not fermented by the culture. The preferred Lactobacillus casei subspecies alactosus is NRRL-B-12,344 or strains having low temperature food fermentation characteristics in common with this strain. In order to provide rapid fermentation, the stimulatory, food grade metal salt, usually a manganese salt, is provided in the food or the culture which is added to the food with the selected lactobacillus to accelerate fermentation. The cultures are particularly suited for the controlled fermentation of carbohydrates, naturally present in or added to the food to provide a selected final pH.
    Type: Grant
    Filed: February 22, 1983
    Date of Patent: April 30, 1985
    Assignee: Microlife Technics, Inc.
    Inventor: Moshe Raccach
  • Patent number: 4407828
    Abstract: A method is described for producing fermented foods by generating lactic acid in the food using a culture of a lactobacillus similar to Lactobacillus casei subspecies alactosus NRRL-B-12,344 and a stimulatory food grade metal salt, wherein the culture has unique rapid low temperature fermentation characteristics and wherein lactose, glycogen, and starch are not fermented by the culture. The preferred Lactobacillus casei subspecies alactosus is NRRL-B-12,344 or strains having low temperature food fermentation characteristics in common with this strain. In order to provide rapid fermentation, the stimulatory, food grade metal salt, usually a manganese salt, is provided in the food or the culture which is added to the food with the selected lactobacillus to accelerate fermentation. The cultures are particularly suited for the controlled fermentation of carbohydrates, naturally present in or added to the food to provide a selected final pH.
    Type: Grant
    Filed: May 29, 1981
    Date of Patent: October 4, 1983
    Assignee: Microlife Technics, Inc.
    Inventor: Moshe Raccach
  • Patent number: 4294930
    Abstract: A process is provided for improving the recovery of microbial cell biomass, which includes the steps of subjecting a culture broth containing chain-forming microorganisms to shearing conditions sufficient to shorten the chain-length of microbial cells whereby the packed cell volume of the cell biomass is substantially reduced and then recovering the thus treated biomass from the broth by centrifugation.
    Type: Grant
    Filed: August 22, 1979
    Date of Patent: October 13, 1981
    Assignee: Miles Laboratories, Inc.
    Inventors: Jeffrey T. Barach, Leslie Bluhm
  • Patent number: RE40023
    Abstract: A pharmaceutical composition containing several different bacteria including Streptococcus thermophilus, Lactobacilli and Bifidobacteria is disclosed. The bacteria are present in the composition at a total concentration of 1×1011 to 1×1013 per gram. Further, methods of using the pharmaceutical are disclosed which include treatment of a gastrointestinal disorder and hypercholestermia. Also a method for modulating a host's immune response is disclosed.
    Type: Grant
    Filed: March 15, 2006
    Date of Patent: January 22, 2008
    Inventor: Claudio DeSimone