Abstract: A process for producing highly concentrated syrups by enzyme treatment of impure starch flour containing beta-glucans. High protein meal and cereal germ oils are recoverable as by-products. The impure starch source is ground into flour and slurried with water. Beta-glucanase enzyme is then added and allowed to react. The reacted slurry is treated with alpha-amylase enzyme and is again allowed to react. The solids and cereal germ oils are separated from the aqueous solution and processed into useful by-products. The aqueous solution is then saccharified into a high dextrose solution by the addition of glucoamylase enzyme. The saccharified mixture is filtered, purified and its pH is adjusted to 8.5 with active MgO before isomerization into a high fructose sweetener by conventional means.

Abstract: Process for preparing fructose by treating starch with alpha-amylase, contacting the resulting liquefied starch with glucoamylase to hydrolyze said starch to glucose, and isomerizing at least part of the resulting glucose to fructose by contacting said glucose with glucose isomerase. The three enzymes are obtained from an organism of the Basidiomycetes class of fungi.

Abstract: A concentrated stillage with a high solids content may be recovered in such a manner that starch or starch-containing raw materials in a suitably crushed form are coaxially introduced into the center of a homogenizer together with enzymes, which are suitable for making alcohol, while simultaneously introducing steam and are suddenly gelatinized therein mechanically and thermally at temperatures of not less than 50.degree. C. and not more than 115.degree. C. while ultrafinely dividing the enzyme; the starch paste after liquefaction is converted to the glucose and the resultant mash is fermented in such a manner that the stillage obtained with reduced dry matter, after removal of merely the coarse contaminations by, for example, centrifuging is repeatedly recycled without further purification and reused as process liquor.

Abstract: This invention relates to a starch hydrolysate which can be optionally hydrogenated as well as the process for preparing said hydrolysate and uses thereof.Its glucidic spectrum corresponds to:a content of monosaccharides (DP=1) less than 14%,a content of disaccharides (DP=2) less than 35%, preferably less than 20%,a content of oligosaccharides of DP 4 to DP 10 ranging from 42% to 70%, preferably from 42 to 60%, the oligosaccharides of DP 5 to DP 7 representing by themselves a proportion preferably higher than 25% and more preferably higher than 30%,a content of polysaccharides of DP higher than 10, less than 32%, and preferably less than 25%.The hydrolysate is useful in the preparation notably of human foodstuff.

Abstract: Alkali metal glucoheptonate is produced from sweet potatoes by reducing the size of sweet potatoes to particles of about 1/16 inch, slurrying the sweet potato particles, adding a-amylase to the slurry, boiling the slurry and filtering to separate a liquid syrup and a protein-containing pulp residue, cooling the liquid syrup and adding glucoamylase, boiling the resulting liquid syrup, and adding an alkali metal cyanide to the liquid syrup and heating to produce the alkali metal glucoheptanate. The alkali metal glucoheptanate is suitable for use as a water conditioning chelant and the protein-containing pulp residue is suitable for use as animal food.

Abstract: Disclosed is a process whereby enzymes are immobilized on activated granular carbon. The process involves treating the carbon with a polyamine compound having pendant amino groups to cause the polyamine to adhere to the carbon leaving pendant amine groups free to further react. The free amine groups are derivatized by treatment with a difunctional compound having amine reactive moieties, so that free amine groups of the enzyme can be covalently bound to the polyamine via the amine reactive compound. By this method, enzymes can be immobilized onto granular carbon which provides a support having excellent physical properties.

Abstract: In a method for the recovery of enzymes, the activity of which after the treatment of starch-containing raw materials used for the production of fermentation alcohol is still not exhausted, the liquid phase containing solutions with still active enzymes after the first separation of the protein fraction is led to a fermentation, then a distillation. The wash water for the protein fraction is provided with a portion of the total amount of alpha-amylase necessary for starch hydrolysis of the raw material and then solids are again separated. The enzyme-containing wash water for the second separated solids is led back into the first enzymatic degradation stage simultaneously with a portion of the slop coming from the distillation.

Abstract: Process for preparing fructose from liquefied starch by contacting the liquefied starch with glucoamylase to hydrolyze said starch to glucose and contacting the glucose so produced with glucose isomerase to isomerize at least a part of the glucose to fructose. The glucoamylase and glucose isomerase are obtained from an organism of the Basidiomycetes class of fungi.

Abstract: An immobilized enzyme product is produced by contacting an inert particulate support with an aqueous enzyme solution containing preferably at least 25% dissolved solids and with a water miscible organic solvent, and crosslinking the enzyme to insolubilize the enzyme on the support as a gel containing 50-90% water. The water miscible organic solvent is in substantial excess of the amount of water mixed with the support in the process. The solvent may be contacted with the support before or after contacting with the enzyme solution. The resultant immobilized enzyme product has a bulk volume 5 to 300% greater than the bulk volume of the support material. A least a portion of the gel is external to the support and constitutes at least 3% by volume of the immobilized enzyme product.

Abstract: This invention relates to a process for producing .gamma.-cyclodextrin without using any organic solvents i.e. by non-solvent process and more particularly, to processes for producing .gamma.-cyclodextrin, which comprise: (1) passing a sugar solution containing cyclodextrins and reducing sugars as primary ingredients through a column packed with alkali or alkali earth metal salts of strongly acidic cation exchange resin to separate cyclodextrins fraction from reducing sugars fraction and then, passing the cyclodextrins fraction through a column packed with gel resin particles to separate and collect .gamma.-cyclodextrin and, (2) bringing the sugar solution into contact with a hydrophobic, synthetic adsorption resin comprising a porous polymer to adsorb only .gamma.-cyclodextrin or .gamma.- and .beta.-cyclodextrins and then, eluting the adsorbed cyclodextrin(s) with water to separate and collect .gamma.-cyclodextrin.

Abstract: Thermal deactivation of amyloglucosidase is substantially retarded at elevated pressures relative to atmospheric pressure. The productivity of glucose formation from amyloglucosidase-catalyzed hydrolysis of starch or partially hydrolyzed starch is significantly increased when the hydrolysis is performed at a pressure greater than about 500 psig.

Abstract: Starch-containing raw materials for the saccharification can be ground at a higher rate and with more economy of energy by mashing the material with hot water, pre-gelatinizing it and then grinding it in the shearing field of a rotor-stator machine having intermeshing radial surfaces. Preferably, enzymes are added already to the mash and hot stillage is used as hot water. Starting material having different grain sizes is previously screened and introduced into the process at different points.

Abstract: A liquid starch solution suitable for saccharification into a maltose-containing product is obtained by suspending starch in water, adding to the starch suspension a heat resistant .alpha.-amylase and a buffer, adjusting the resultant mixture to a pH value in the range of from 7.5 to 8.0, and thermally treating the resultant starch milk while repressing possible hydrolysis of starch molecules.

Abstract: This invention relates to a process for producing cyclodextrins, which comprises passing a solution containing cyclodextrins and reducing sugars as primary ingredients through a column packed with alkali or alkali earth metal salts of strongly acidic cation exchange resin to separate and collect cyclodextrins from the sugar solution.

Abstract: Disclosed is a dry or wet milling process for making fermentable sugars and high-protein products from starch bearing material, characterized by saccharification of the starch followed by recovery of fiber and other non-protein materials and of destarched protein as separate products, leaving a sugar solution that is essentially free of insoluble materials. The sugars are fermented to ethanol and carbon dioxide by the action of added yeast. After fermentation, the yeast is recovered for use in fermenting additional quantities of sugars supplied either for batch or continuous processing. The alcohol is then removed leaving a dilute solution of unfermentable soluble materials, and this liquid, in whole or in part, is recycled to preceding steps in the process.

Abstract: An aqueous starch slurry is hydrolyzed in sequential liquefication and saccharification steps to provide saccharified starch containing from about 60 to about 80 weight percent of fermentable sugar based on the weight of the original starch present. Further saccharification is carried out either in one or more fermentation vessels wherein conversion of the sugar to ethanol simultaneously takes place or in one or more additional saccharification vessels.

Abstract: Starch degrading enzymes are immobilized by modifying or cross-linking the starch-degrading enzymes with a mono- or poly-functional reagent in a manner that does not substantially insolubilize the enzymes and then physically adsorbing the resultant modified or cross-linked enzymes onto a water-insoluble carrier. The resultant immobilized enzymes have high activity and the carrier can be easily recovered for repeated use.

Abstract: Saccharifying starch hydrolysate to a high DX glucose syrup at pH 3-5 by the enzyme mixture of a glucoamylase and an acidophilic iso-amylase. A lower dosage level than the heretofore conventional glucoamylase dosage may be employed and higher DX glucose syrups can be obtained.

Abstract: This invention relates to a novel fermentation process and in particular, to fermentative production of ethanol producing in the presence of non-gelled, or granular, starch particles, alpha-amylase and a glucoamylase; characteristic of the present process is recycle of enzymes for renewed use in fermentation, usually through termination of the fermentation prior to complete disappearance of the granular starch particles, and recovery for use anew of the unconsumed starch, along with enzymes thereon. Fermentation according to practice of this invention proceeds at a near to linear rate in the main.

Abstract: Heat and acid-stable alpha-amylase enzymes having the following characteristics: (1) capable of retaining at least about 70% of their initial activity when held at 90.degree. C. and at a pH of 6.0 for 10 minutes in the absence of calcium ion; (2) capable of retaining at least about 50% of their initial activity when held at 90.degree. C. at a pH of 6.0 for 60 minutes in the absence of added calcium ion; and/or (3) capable of retaining at least about 50% of their initial activity at a temperature of 80.degree. C. and at a pH of 4.5 in the presence of 5 mM calcium ion for 10 minutes. The preferred alpha-amylases are prepared by culturing a strain of a Bacillus stearothermophilus microorganism in a suitable culture medium. The novel alpha-amylases are useful in hydrolyzing and/or liquefying starch and due to their stability at low pH values they can be used in conjunction with other acid stable amylases such as gluco-amylase in either a soluble or an immobilized form.

Abstract: The present invention relates to a process for the preparation of hydrolyzed products from whole grain, and such derived products. The invention solves the problem of obtaining a protein and sugar containing product able to be filtrated whereby this is achieved by treating whole grain, such as wheat, maize, rye, barley, oat, and rice, with a proteolytic enzyme to transform water insoluble proteins into watersoluble products, and further to treat the starch contents with an amylase free from other carbohydrate hydrolyzing enzymes to form water-soluble starch products, as mono and disaccharides, removing the bran fraction and removing water to obtain a dry, semimoist, or liquid but concentrated derived product. The product is to be added as a sweetening agent in food products as bread, drinks, and cereal products, whereby the bran obtained can be used in bread as fiber additive.

Abstract: Process for the production of a glucoamylase having a pH optimum at about 6.0 to 6.5 by a strain of Stachybotrys and the glucoamylase produced thereby.

Abstract: In a process for producing a foodstuff, more particularly a fodder from cereal, it is disclosed to saccharify the starch contained in the cereal to form dextrose by enzymatic degradation of the starch in the cereal grain. All the cereal substances, both those containing starch and those not containing starch, are treated together in the process of enzymatic degradation of the starch in the cereal grain until the starch has been converted to dextrose in the cereal grain. The product is then dried.

Abstract: Process for the production of a thermostable glucoamylase by a strain of Talaromyces and the glucoamylase produced thereby.

Abstract: A process for producing highly concentrated syrups by enzyme treatment of impure starch flour containing beta-glucans. High protein meal and cereal germ oils are recoverable as by-products. The impure starch source is ground into flour and slurried with water. Beta-glucanase enzyme is then added and allowed to react. The reacted slurry is treated with alpha-amylase enzyme and is again allowed to react. The solids and cereal germ oils are separated from the aqueous solution and processed into useful by-products. The aqueous solution is then saccharified into a high dextrose solution by the addition of glucoamylase enzyme. The saccharified mixture is filtered, purified and its pH is adjusted to 8.5 with active MgO before isomerization into a high fructose sweetener by conventional means.

Abstract: A culture filtrate of Cladosporium resinae (Strain ATCC No. 20495) has been found to contain a mixture of starch-degrading enzymes capable of bringing about efficient conversion of starch and pullulan into glucose. Culture conditions resulting in optimal production of the pullulan degrading activity been established. The amylolytic enzyme preparation obtained by culturing the fungus under these optimal conditions has been fractionated by ion-exchange and molecular sieve chromatography and shown to contain at least four enzymes, a maltase, .alpha.-amylase and two glucoamylase-type enzymes including a novel exo-pullulanase. The maltase and glucoamylase enzymes have been purified to homogeneity and their substrate specificity investigated. Both the mixture and the exo-pullulanase can be used in the manufacture of dextrose from starch.

Abstract: A process for the production of dextrose-containing syrups wherein a deionized starch hydrolyzate is treated with glucoamylase immobilized on porous alumina.

Abstract: A sweetener containing alpha-glycosyl stevioside which is obtained by allowing alpha-glucosyltransferase to react on an aqueous solution containing stevioside and alpha-glucosyl sugar compound.

Abstract: A mixture of vital wheat gluten and starch containing at least 25% protein, e.g. a protein-rich fraction of wheat flour, is treated with a bacterial alpha-amylase (preferably substantially free of protease) under conditions which solubilize the starch but do not unduly solubilize, or denature, the vital gluten. Specifically, the temperature should be not above about 80.degree. C. and the time of treatment should not exceed about 6 hours. After the enzymatic treament the gluten, which still retains its vital properties, is separated out, and the solubilized starch fraction is recovered or subjected to further processing.

Abstract: Complexes of metals with chelating resins are described. In the principal embodiment the complex is a complex of a resin, an enzyme and a metal that is in non-ionic form and that is chelated by the resin and the enzyme. Such material is of particular value for converting dextrin to glucose. In another embodiment a solid agricultural composition is formed comprising a complex of an agriculturally acceptable trace metal chelated by a chelating resin and finely divided solid carrier.

Abstract: Sweet, non-crystallizing (at concentrations of 75% solids or above) syrups are prepared having the following saccharide composition: dextrose, from 20% to 40%; levulose, from 20% to 40%; maltulose, from 10% to 60%; total ketose composition (principally levulose and maltulose), 40% to 80% (percentages by weight dry basis). Optionally, the syrups may contain up to 25% maltose and/or up to 20% higher saccharides (i.e. having degrees of polymerization of greater than 2). The syrups are prepared by first subjecting a starch hydrolyzate, containing at least 40% maltose and not more than 5% dextrose, to an alkaline isomerization treatment to isomerize a portion of the maltose to maltulose, the isomerization being conducted until the resulting hydrolyzate contains between 10% and 60% maltulose. The resulting product is then treated with glucoamylase to saccharify at least a portion of the remaining maltose (as well as higher saccharides, if present) to dextrose.

Abstract: Saccharification of low DP polysaccharides in syrups of high fructose content and low glucose content by short term contact with amyloglucosidase e.g. in less than 60 minutes, 1-10 AG units/gm of syrup solids, and syrup concentrations of 2-50 w/o solids in a continuous process employing immobilized AMG.Suitable high fructose content low glucose syrups are products that result from fractionation of isosyrup into enriched 50+% d.s.b. fructose syrups.

Abstract: Saccharification of low DP polysaccharides of high glucose content syrups by short term contact with amyloglucosidase e.g. 1-10 hours, 1-10 AG units/gm of syrup solids, and syrup concentrations of 5-25 w/o solids in a batch saccharification, less than 30 minutes in a continuous process employing immobilized AMG.Suitable high glucose content syrups are co-products that result from fractionation of isosyrup into 50+% d.s.b. fructose syrups and from production of crystalline dextrose.

Abstract: A process for production of dextrose from starch wherein a starch hydrolyzate produced using soluble .alpha.-amylase or a combination of soluble glucoamylase and .alpha.-amylase is treated with glucoamylase immobilized on a cationic colloidal silica to produce a dextrose-containing syrup.

Abstract: Crystalline maltose in good yields is obtained by subjecting a starch hydrolyzate having a maltose content of at least 75% and a viscosity of greater than 400 cps. to the action of glucoamylase, whereby the viscosity is reduced to below 400 cps. without any substantial reduction in the maltose content, and then subjecting the resulting hydrolyzate to a conventional crystallization operation.

Abstract: A method and system for the automatic determination of the weight of starch or similar substances in various forms of simple or complex substrates. In a system in accordance with the invention, dispersion of starch is effected by ultrasonics and enzymatic hydrolysis is carried out continuously in segmented form. The colorimetric determination of glucose obtained thereby is also effected continuously in segmented form.

Abstract: Sweet, non-crystallizing (at concentrations of 75% solids or above) syrups are prepared having the following saccharide composition: dextrose, from 20% to 40% levulose, from 20% to 40%; maltulose, from 10% to 60%; total ketose composition (principally levulose and maltulose), 40% to 80% (percentages by weight dry basis). Optionally, the syrups may contain up to 25% maltose and/or up to 20% higher saccharides (i.e. having degrees of polymerization of greater than 2). The syrups are prepared by first subjecting a starch hydrolyzate, containing at least 40% maltose and not more than 5% dextrose, to an alkaline isomerization treatment to isomerize a portion of the maltose to maltulose, the isomerization being conducted until the resulting hydrolyzate contains between 10% and 60% maltulose. The resulting product is then treated with glucoamylase to saccharify at least a portion of the remaining maltose (as well as higher saccharides, if present) to dextrose.