Stabilizing Or Preserving Patents (Class 436/176)
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Patent number: 7598089Abstract: The invention provides compositions and methods for cell separation. These reagents and techniques specifically agglutinate cells via surface antigen recognition and can be used to recover rare cell types in high yield.Type: GrantFiled: April 19, 2006Date of Patent: October 6, 2009Assignee: BioE, Inc.Inventor: Daniel P. Collins
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Patent number: 7592179Abstract: The present invention provides a method for preparing a five-part differential white blood cell control and a white blood cell control prepared thereby. The white blood cell control obtained by the method of the present invention perfectly retains the light scattering properties of every type of white blood cells and has much higher stability than that of real white blood cells, and therefore can be used for the quality control of five-part differential hematology analyzers based on the principle of multi-angle light scattering.Type: GrantFiled: October 15, 2007Date of Patent: September 22, 2009Assignee: Shenzhen Mindray Bio-Medical Electronic Co., Ltd.Inventor: Xiaonan Guo
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Patent number: 7589184Abstract: The invention provides compositions and methods for storage of biomolecules. The biomolecules are stored via absorption to a substrate. Absorbed biomolecules can be eluted or recovered from the substrate at a future time, and optionally be subjected to a subsequent analysis or application. Biomolecules absorbed to a substrate for storage may also optionally be preserved, i.e., the absorbed biomolecule is resistant to or resists degradation.Type: GrantFiled: May 24, 2005Date of Patent: September 15, 2009Assignee: GenVault CorporationInventors: Michael Hogan, James C. Davis
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Publication number: 20090197346Abstract: The present invention concerns methods and compositions to prepare biological samples to preserve the macromolecules in the samples. Embodiments of the invention concern the use of a soak solution that contains one or more water-miscible solvents. A sample is incubated with the soak solution to the point of saturation at a temperature above the melting temperature of the water-miscible solvent but below 0° C. The use of methods and compositions of the invention allow for subsequent preparation or analysis of the samples.Type: ApplicationFiled: February 23, 2009Publication date: August 6, 2009Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Matthew M. Winkler, Richard C. Conrad
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Patent number: 7560287Abstract: Engineered fluorescent proteins, nucleic acids encoding them and methods of use are provided.Type: GrantFiled: August 23, 2004Date of Patent: July 14, 2009Assignees: The Regents of the University of California, State of Oregon acting by and through the State Board of Higher Education on behalf of the University of OregonInventors: Roger Y. Tsien, S. James Remington, Andrew B. Cubitt, Roger Heim, Mats F. Ormö
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Patent number: 7560282Abstract: The present invention relates to the qualitative and quantitative validation of marker indices, especially for medical diagnosis and particularly for the determination of the growth fraction in a sample with antibodies against the Ki-67 protein. Diagnostic kit for the quantification of a cell fraction labeled by a marker for in vitro diagnosis, characterized in that a pseudo-tissue is used for the intra- and inter-assay standardization of the marker index.Type: GrantFiled: January 23, 2001Date of Patent: July 14, 2009Assignee: DakoCytomation Denmark A/SInventor: Johannes Gerdes
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Patent number: 7541161Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway.Type: GrantFiled: February 10, 2004Date of Patent: June 2, 2009Assignee: University of MassachusettsInventor: Andrew H. Fischer
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Patent number: 7517697Abstract: The present invention concerns methods and compositions to prepare biological samples to preserve the macromolecules in the samples. Embodiments of the invention concern the use of a soak solution that contains one or more water-miscible solvents. A sample is incubated with the soak solution to the point of saturation at a temperature above the melting temperature of the water-miscible solvent but below 0° C. The use of methods and compositions of the invention allow for subsequent preparation or analysis of the samples.Type: GrantFiled: February 5, 2003Date of Patent: April 14, 2009Assignee: Applied Biosystems, LLCInventors: Matthew M. Winkler, Richard C. Conrad
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Publication number: 20090042312Abstract: A method of testing a compound for its capability of quenching singlet excited state energy from a photon-excited photoactive compound, thereby photostabilizing said photoactive compound, comprising mixing: (1) a composition comprising a photoactive compound in a solvent and determining the degree of fluorescence of said composition (1) by exposing the composition (1) to UV radiation in an amount sufficient for the photoactive compound to reach an electronic singlet excited state, and (2) a mixture comprising said photoactive compound with a test compound in a solvent, and determining the degree of fluorescence of said mixture (2) by exposing the mixture to UV radiation to the same degree as composition (1), and comparing the degree of fluorescence of composition (1) with mixture (2).Type: ApplicationFiled: August 9, 2007Publication date: February 12, 2009Applicant: HALLSTAR INNOVATIONS CORP.Inventor: Craig A. Bonda
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Patent number: 7482161Abstract: Red blood cells from a vertebrate are treated to make them effective components of a hematology control, allowing the control to be used for detecting all blood cell components, including white blood cells and platelets. The treatment includes the use of a fixative under limited conditions of concentration and exposure time, and the resulting red blood cells are stable but lysable in a hematology instrument and have a reduced tendency to form particulates.Type: GrantFiled: July 13, 2007Date of Patent: January 27, 2009Assignee: Bio-Rad Laboratories, Inc.Inventors: Franklin J. Carver, Lorraine Granier
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Patent number: 7452724Abstract: In order to ensure optimum metering of a reagent to be metered into an exhaust gas during operation of a metering unit of a catalytic converter of a combustion system, in particular an internal combustion engine of a motor vehicle, in any operating state of the catalytic converter and/or in any operating state of the combustion system, a method and a device for operating a metering unit of a catalytic converter of a combustion system provide that, based on a steady-state value of the reagent quantity to be metered during a steady-state operating state of the catalytic converter and/or the combustion system, the quantity of the at least one reagent is determined and adjusted using at least one dynamic correction factor which is dependent on at least one of the performance characteristics of the catalytic converter and on at least one of the performance characteristics of the combustion system.Type: GrantFiled: January 20, 2004Date of Patent: November 18, 2008Assignee: Robert Bosch GmbHInventor: Stefan Wickert
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Patent number: 7449341Abstract: The invention concerns a method for isolating an analyte from an initial sample containing same, on a reactive surface in contact with all or part of the initial sample, characterized in that it comprises the following steps which consist in: 1) forming and/or maintaining a reaction volume entity, comprising an internal liquid medium, corresponding to all or part of the initial sample, the entity including an interface with an external medium, having with the latter a surface tension; 2) applying a temperature difference between at least two thermal points located: a) proximate to and/or b) on the surface and/or c) within said entity, said thermal points being respectively different, in terms of temperature and positioning; 3) fixing the analyte on the reactive surface, positioned on the path of forces convection generated by the temperature difference.Type: GrantFiled: September 12, 2002Date of Patent: November 11, 2008Assignees: Biomerieux, Commissariat a l'Energie AtomiqueInventors: Frederic Ginot, Jean-Luc Achard, Laurent Drazek, Pascale Pham
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Patent number: 7416892Abstract: A microfluidic system for isolation and amplification of DNA or RNA from aqueous solutions and detection of the DNA or RNA on a lateral flow detection strip, including a disposable microfluidic card for use in analysis of bacteria in platelets and an analysis of sexually transmitted diseases (STD) in urine. The card will include an embedded membrane that filters out cells and cellular debris. Any biological debris on the membrane will be lysed and the DNA or RNA amplified via PCR amplification protocol, including appropriate reagents and thermal cycling conditions. The amplified DNA or RNA are transferred to a lateral flow detection strip for a visual diagnostic read out. An alternate embodiment includes a microfluidic card for use in typing antiglobulin assays.Type: GrantFiled: January 21, 2004Date of Patent: August 26, 2008Assignee: Micronics, Inc.Inventors: C. Frederick Battrell, Mingchao Shen, Bernhard H. Weigl, Jeffrey M. Houkal, Christy A. Lancaster, Wayne Breidford
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Patent number: 7413908Abstract: A gel extraction device comprising a hollow cutting member having a cutting edge at one end and a squeeze bulb at the other end. In a further embodiment, the air passage between the cutting edge and the bulb has a constriction zone to prevent any extracted gel from being drawn too deeply into the extractor. In a further embodiment, a blow-hole in the hollow cutting member or in the squeeze bulb provides for the passage of air displaced by gel through the extractor. The blow-hole may be covered to secure the gel in the receptacle for transfer from the matrix to a sample container.Type: GrantFiled: December 20, 2005Date of Patent: August 19, 2008Inventors: Jeremy Caldwell, Dale Caldwell, Leon Clouser
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Patent number: 7410753Abstract: The present invention provides reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents comprise components optimized to facilitate removal or etching of the embedding media from the biological sample. The present invention also provides reagents for use in an automated environment for cell conditioning biological samples wherein the cells are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents comprise components optimized to facilitate molecular access to cells and cell constituents within the biological sample.Type: GrantFiled: March 21, 2003Date of Patent: August 12, 2008Assignee: Ventana Medical Systems, Inc.Inventors: Keri Hopkins, Alexis Pusch, Catherine Wolf, Anthony Hartman, James Pettay, Kimberly Christensen, Ethel Macrea, Noemi Sebastiao
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Patent number: 7405083Abstract: The subject invention provides a sample processing technique for purifying a biological or chemical sample. The invention is particularly well-suited to prepare a sample for mass spectrometry. The process is to be performed in an article having at least one well, in which the surface of the well is at least partially hydrophobic and/or modified with bio-specific ligands. Targeted solutes, such as salts or small molecule contaminants, can be removed from a solution to allow for a purified solution of a desired type of solute, such as peptides and/or proteins.Type: GrantFiled: May 13, 2004Date of Patent: July 29, 2008Assignee: Becton, Dickinson and CompanyInventor: Xiaoxi (Kevin) Chen
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Patent number: 7393694Abstract: A self-contained, leak-proof sampling device and kit employing said device for collecting chemical and biological samples from various surfaces. The invention provides a sampling device and kit that may be employed to easily collect chemical and biological samples, safely transport the collected samples, safely dispense collected samples for analysis and provide optimum sample recovery. The sampling device is in the form of a leak proof container that comprises a lid and base. The lid contains a sterile absorbent collection means integrated into and positioned on the inside surface of the lid. The base contains a means to facilitate sample recovery from the absorbent collection means via compression and/or scraping of the absorbent collections means. Methods for employing the present invention are described herein.Type: GrantFiled: October 26, 2005Date of Patent: July 1, 2008Assignee: The United States of America as represented by the Secretary of the ArmyInventors: Mark S. Schlein, Peter A. Emanuel, Kevin S. Wallace, Peter J. Schlitzkus
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Patent number: 7393693Abstract: A sensor and method for making a sensor includes providing a substrate having an adhesion layer with a first functional group operatively associated with a sol-gel composition having a second functional group, and at least one linker material for connecting the first functional group to the second functional group. In another embodiment, a sensor and method for making the sensor includes providing a sol-gel layer operatively associated with a sol-gel precursor solution that includes at least one co-polymer and at least one solvent adapted to resist the deformation of the sol-gel layer by decreasing evaporation, decreasing cross-linking, increasing intermolecular forces, or increasing elasticity.Type: GrantFiled: May 31, 2002Date of Patent: July 1, 2008Assignee: Agilent Technologies, Inc.Inventors: David J. Anvar, Ganapati R. Mauze, Dan-Hui Yang
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Patent number: 7390663Abstract: The present invention is directed toward a stable calibrator and/or control, kit and process for using in a glucose monitoring instrumentation. Principally, the instant invention teaches a glycolyzed red blood cell component which has been treated with a glycolysis stabilizing effective amount of at least one non-crosslinking aldehyde compound which may be added to fresh plasma along with an amount of glucose to form a simulated whole blood glucose control product, effective for maintaining a particular and essentially stable glucose concentration over a period of time sufficient for accurate measurement and calibration of a glucose measuring instrument.Type: GrantFiled: February 23, 2006Date of Patent: June 24, 2008Assignee: Streck, Inc.Inventors: Wayne L. Ryan, Bradford A. Hunsley
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Patent number: 7387880Abstract: The amount of platelet surface proteins in a sample may be measured by collecting a sample containing platelets into a collection tube containing a platelet stabilizing composition, labeling the platelet surface protein and detecting by cytometry.Type: GrantFiled: March 7, 2005Date of Patent: June 17, 2008Assignee: PPD Biomarker Discovery Sciences, LLCInventors: Timothy L. Wyant, Paul A. Raju, Aaron B. Kantor
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Patent number: 7354767Abstract: A reference control composition containing a nucleated red blood cell component and the method of making are disclosed. The reference control composition includes a nucleated red blood cell component made of fixed non-nucleated blood cells and a suspension medium. The non-nucleated blood cell has a natural cell size substantially similar to a size of nucleus of said nucleated red blood cell of said blood sample. The nucleated red blood cell component can be made of equine, ovine, bovine, feline, canine, or porcine red blood cells; and it is substantially free of nucleic acid. The reference control composition can further include a white blood cell component, a red blood cell component, a platelet component, a reticulocyte component, or combinations thereof. Further disclosed are the methods of using the reference control composition for measurement of nucleated red blood cells on a blood analyzer.Type: GrantFiled: March 16, 2006Date of Patent: April 8, 2008Assignee: Beckman Coulter, Inc.Inventors: Nery Ortiz, Theodore J. Gerula, Sandra Socarras
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Publication number: 20080070320Abstract: A method of detecting organic vapors is described. More particularly, the method involves the use of an analyte sensor that contains a polymeric material having a relatively large intrinsic porosity and that is capable of fluorescence in the visible region of the electromagnetic spectrum. The method further includes exposing the analyte sensor to an environment that may contain an organic vapor and monitoring the analyte sensor for a change in a fluorescence signal. Although the organic vapor itself typically does not fluoresce in the visible wavelength range, presence of an organic vapor can alter the fluorescence signal of the analyte sensor.Type: ApplicationFiled: October 25, 2006Publication date: March 20, 2008Inventors: Michael C. Palazzotto, Neal A. Rakow, Michael S. Wendland
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Patent number: 7338809Abstract: In a method for measuring an analyte, which comprises a reaction step of forming a reaction system including a sample containing whole blood, a first substance carried by a solid carrier and specifically binding to an analyte contained in the sample and a second substance specifically binding to the analyte and allowing the analyte to react with the first and second substances and a measurement step of measuring the formed reaction product, (1) the reaction step is performed in a state that blood cells are not disrupted, and (2) at least the reaction step is performed in the presence of a sufficient amount of a detergent that does not cause hemolysis, does not inhibit reactions of the analyte with the first and second substances specifically binding to the analyte and can prevent influence on the reaction system of a component existing in the reaction system.Type: GrantFiled: September 9, 2003Date of Patent: March 4, 2008Assignee: Mitsubishi Kagaku Iatron, Inc.Inventor: Hiroyuki Yokoi
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Patent number: 7329533Abstract: The invention relates to a unit for the treatment of histological samples. The unit comprises a treatment chamber (1), a device (2) for supplying and removing treatment agents (3, 3?, 3?) to and from the treatment chamber (1), and a control system (4) for the performance of the treatment. The aim of the invention is to develop the unit in such a way that the need to carry out different treatments is taken into account in an economical manner. Towards this end, at least one other treatment chamber (5) is provided, by means of which treatments can be carried out independently from the first treatment chamber (1), the device (2) and the control system (4) also co-operating with that other treatment chamber (5).Type: GrantFiled: December 11, 2002Date of Patent: February 12, 2008Assignee: Microm International GmbHInventor: Jerry Fredenburgh
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Patent number: 7326577Abstract: A new method of cell fixation using a mild denaturing heat and the fixative PERMIFLOW™ or similar fixative that preserves cell morphology, light scatter profiles, nucleic acid content, cell surface epitopes and unmasks internal epitopes previously not available. The method can be combined with analysis of each of these parameters by flow cytometry and thus has application in drug screening and patient or tumor monitoring protocols.Type: GrantFiled: August 13, 2004Date of Patent: February 5, 2008Assignee: Esoterix, Inc.Inventors: Keith E. Shults, Angel L. Flye
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Patent number: 7323345Abstract: The present invention relates to a microfluidic device comprising a microchannel (2, 4) providing for solvent contact between an open microarea (MA) carrying a microvolume (1) of a solvent and a reservoir (3) for the solvent, said reservoir (3) and said microchannel (2, 4) being adapted so that solvent evaporated from said microarea (MA) is continuously replaced by solvent from the reservoir (3) through said microchannel (2, 4). It further relates to method for replacing solvents evaporating from a microvolume (1) of solvent placed in an open microarea (MA) of a microfluidic device, wherein replacement is continuously taking place via a microchannel (2, 4) that transports solvent to the microarea (MA) from a solvent reservoir (vessel) (3). The device and method are suitable for preventing the desiccation of samples.Type: GrantFiled: October 29, 1999Date of Patent: January 29, 2008Assignee: Norada Holding ABInventor: Mårten Stjernström
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Patent number: 7319032Abstract: The present invention provides methods, compositions, and kits for rapid detection of analytes in a sample. Surprisingly, it has been found that bulking materials having non-sugar sweeteners effectively enhance chromatographic detection methods by providing component stability and controlled release. Thus, the novel components and methods disclosed herein provide a completely new modality of chromatographic analyte detection.Type: GrantFiled: September 21, 2004Date of Patent: January 15, 2008Assignee: MedToxInventors: Robert Bohannon, Glen Chapman, Alan Morris, Maria Scholz-Steele, Martin Green, Phillip Hartzog, Oluyomi Adeneye
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Patent number: 7309468Abstract: A collection container and a method for collecting a biological sample, particularly whole blood, that includes at least one stabilizing agent in an amount effective to stabilize and inhibit protein degradation and/or fragmentation. The stabilizing agent is able to stabilize proteases in the biological sample, particularly at the point of collection, by inhibiting protein degradation and/or fragmentation in the sample when the sample is stored. The stabilizing agent includes one or more protease inhibitors.Type: GrantFiled: May 13, 2003Date of Patent: December 18, 2007Assignee: Becton, Dickinson and CompanyInventors: Timothy Stevens, Bruce Haywood, Ajit Dastane, Fu-Chung Lin
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Patent number: 7306950Abstract: The invention relates to a composition for the stabilization of sulphur-containing amino acids and/or for the inhibition of the continuous formation of sulphur-containing amino acids in withdrawn blood, to the use of suitable substances and compositions therefor as well as optionally for the determination of sulphur-containing amino acids in blood, to a process for these purposes, as well as to a blood collecting device applied appropriately to these processes.Type: GrantFiled: May 15, 2002Date of Patent: December 11, 2007Assignee: Heinrich WielandInventors: Heinrich Wieland, Emanuel Bissé
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Patent number: 7285426Abstract: The present invention provides methods, compositions, and kits for rapid detection of analytes in a sample. Surprisingly, it has been found that bulking materials having starch reagents effectively enhance chromatographic detection methods by providing component stability and controlled release. Thus, the novel components and methods disclosed herein provide a completely new modality of chromatographic analyte detection.Type: GrantFiled: May 5, 2005Date of Patent: October 23, 2007Assignee: Medtox Scientific, Inc.Inventors: Robert Bohannon, Glen Chapman, Alan Morris, Maria Scholz-Steele, Martin Green, Phillip Hartzog, Oluyomi Adeneye
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Patent number: 7285418Abstract: The invention provides a method for assaying for troponin I in a sample such as serum, the method comprising a determination of the troponin I concentration in a sample using a standard preparation that comprises a complete native troponin complex. One exemplary assay for troponin I concentration according to the invention is an immunoassay. Further contemplated in the method is a step comprising contacting the sample with a Ca2+-binding agent. Another aspect of the invention is the standard preparation comprising a complete native troponin complex. Yet another aspect of the invention is a kit for use in the assay method comprising the standard preparation, a detectable label, and a troponin I binding partner, such as an anti-troponin I antibody. All aspects of the invention are usefully practiced using human materials, such as a human troponin complex, and/or human sources for samples.Type: GrantFiled: July 1, 2002Date of Patent: October 23, 2007Assignee: Hytest Ltd.Inventors: Alex G. Katrukha, Sergei Severin, Anastasia Bersenikova, Tatiana Esakova, Kim Pettersson
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Patent number: 7282371Abstract: The present invention relates to a receptacle for receiving samples, containing a nucleic-acid stabilizing solution and a nucleic-acid-binding solid phase. The receptacle is especially suited for the withdrawal of blood to be examined for nucleic acid.Type: GrantFiled: February 15, 2001Date of Patent: October 16, 2007Assignee: Preanalytix GmbHInventor: Elke Helftenbein
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Patent number: 7264776Abstract: A biological sample collection and test kit and a method of using the kit are disclosed in the application. The kit includes a swab, having a sample collection portion, a cover for covering the sample collection portion of the swab for hygienic purpose. Also disclosed is a package for the swab and the cover.Type: GrantFiled: July 12, 2004Date of Patent: September 4, 2007Inventors: Huiyan Guo, Hsaioho Edward Tung, Shaomin Sun, Yuchang Wu, Rong Yang, Yun Ling
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Patent number: 7214481Abstract: The invention discloses methods, compositions and kits for stabilizing a solubilized phenyl phosphate, preferably paranitrophenyl phosphate (PNPP), using charcoal. Also disclosed are methods, compositions and kits for recycling solubilized phenyl phosphate, preferably PNPP, that has an absorbance of less than 0.1 when measured at 405 nm due to non-enzymatic hydrolysis.Type: GrantFiled: November 1, 2005Date of Patent: May 8, 2007Assignee: BioFX Laboratories, Inc.Inventor: Thomas M. Woerner
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Patent number: 7191670Abstract: A method and system for automated remote microbial air sampling includes a sampling enclosure positioned outside an isolator. An infeed sample receiver passes sample containers into the enclosure. An atrium is located within the enclosure and exposes containers to a gas sample. A remote sampling device collects the gas sample from the isolator and passes it to the atrium. A load arm is located within the enclosure. The load arm intermittently permits containers to enter the enclosure and moves containers to the atrium. An outfeed sample receiver passes containers out of the enclosure. The system allows addition and removal of containers from the air sampling system without interrupting the processing of the containers within the enclosure, and allows the first container supplied to the air sampling system to be the first exposed container removed therefrom.Type: GrantFiled: July 27, 2005Date of Patent: March 20, 2007Assignee: Hospira. Inc.Inventor: Alan H. Aicher
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Patent number: 7150996Abstract: A method and apparatus for determining the stability of dispersions and emulsions accelerates the onset of significant particle agglomeration in a sample by stressing the sample by reducing the height of the interparticle potential energy barrier between the particles. This is achieved by adding one or more of three stress factors: changing the pH of the sample to reduce the surface charge on the particles; adding an adsorbing electrolyte so that ions of the appropriate charge are adsorbed onto the surfaces of the particles to reduce the net charge on the particles; and applying a monovalent, divalent, or trivalent salt to partially screen electrostatic repulsions between the charged particles. In a preferred embodiment, the increase in agglomeration is detected with single particle detection, such as SPOS, to generate a PSD from which a figure of merit is derived. Another embodiment detects turbidity or light scattering to generate a value X indicative of the extent of agglomeration.Type: GrantFiled: June 27, 2003Date of Patent: December 19, 2006Assignee: Stable Solutions, Inc.Inventors: David F. Nicoli, David F. Driscoll, Bruce R. Bistrian
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Patent number: 7112453Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: August 5, 2002Date of Patent: September 26, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7026110Abstract: The invention concerns a reagent for determination of leucocytes and measurement of haemoglobin in a sample of blood. This reagent comprises a buffer system that is suited to adjust selectively the pH of the reagent to an acidic value, at least one detergent of cationic type, a nitrogenous compound and, optionally, at least one inorganic salt. This reagent can be used in haematological analyses in human medicine and also permits the identification of a leucocytic subpopulation, in particular the basophil polymorphonuclear leucocytes.Type: GrantFiled: March 16, 2000Date of Patent: April 11, 2006Assignee: Horiba ABXInventors: Sylvie Veriac, Henri Champseix
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Patent number: 7011940Abstract: The invention describes quality control devices for assays that measure analytes in cells and tissue samples, and methods of use thereof. In particular, the quality control device comprises a matrix affixed with synthetic controls in different concentrations, or different synthetic controls. The quality control device can be adhered to a microscope slide via an adhesive or chemically attached to a microscope slide and processed simultaneously with a tissue sample.Type: GrantFiled: April 14, 2000Date of Patent: March 14, 2006Assignee: Medical Discovery Partners LLCInventors: Seshi R. Sompuram, Halasya Ramanathan, Steven A. Bogen
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Patent number: 7011938Abstract: Platelet activation is measured by determining Mean Platelet Component (MPC) of suspended blood platelets, using a specific anticoagulant composition. The composition comprises at least one component for effecting platelet sphering (for example EDTA), and at least one platelet antagonist (for example at least one of, and preferably all three of theophylline, adenosine and dipyridamole).Type: GrantFiled: November 8, 2001Date of Patent: March 14, 2006Assignee: Barts and The London NHS TrustInventor: Marion G. Macey
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Patent number: 7008797Abstract: The present invention describes a method for recovery of aqueous samples or components thereof from polymer packaging materials, wherein the spilled aqueous sample has been absorbed by the packaging material to form a liquid swollen absorbent gel. The method comprises the steps of contacting the liquid swollen absorbent gel with a recovery fluid, wherein the recovery fluid has a higher osmotic pressure than the aqueous sample.Type: GrantFiled: July 8, 2003Date of Patent: March 7, 2006Assignee: The Research Foundation of State University of New YorkInventors: Paschalis Alexandridis, Sriram Neelamegham
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Patent number: 7001717Abstract: The invention discloses methods, compositions and kits for stabilizing a solubilized phenyl phosphate, preferably paranitrophenyl phosphate (PNPP), using charcoal. Also disclosed are methods, compositions and kits for recycling solubilized phenyl phosphate, preferably PNPP, that has an absorbance of less than 0.1 when measured at 405 nm due to non-enzymatic hydrolysis.Type: GrantFiled: December 5, 2003Date of Patent: February 21, 2006Assignee: BioFX Laboratories, Inc.Inventor: Thomas M. Woerner
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Patent number: 6962819Abstract: A method of measuring an analyte using a dry analytical element which comprises, a process of supplying a measuring reagent solution to the anlaytical element comprising at least a hydrophilic polymer layer and a microporous layer having a surface rendered hydrophilic and having a blood cell-separating ability laminated onto a water-impermeable support and not containing the measuring reagents to which a liquid sample has been supplied and thereby reaction occuring, and a process of measuring the analytical element wherein the reaction occurred by an optical means. According the above method, the shelf life of the analytical element is greatly improved. Besides, since the measuring reagnts used in the conventional wet analysis can be used, it is not necessary to develop analytical elements for each measuring item. Moreover, one kind of analytical element can be applied to many items.Type: GrantFiled: March 20, 1995Date of Patent: November 8, 2005Assignee: Fuji Photo Film Co., Ltd.Inventor: Masao Kitajima
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Patent number: 6946297Abstract: A transport/preservation system has been found which provides safe, effective transport for patient specimens and bodily fluids, and functions as a preservative for biological reagents, therapeutics, and personal care products. The transport/preservative formulations can also be used as safe, effective sanitizers on surfaces, equipment, and appliances. The transport preservation formulation includes a biguanide and one or more other antimicrobial agents, which is cidal to microorganisms when present in a sample or on a surface to be sanitized.Type: GrantFiled: April 5, 2004Date of Patent: September 20, 2005Inventor: Gordon L. Dorn
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Patent number: 6939512Abstract: A heating and cooling device (3) for an apparatus (1) for tissue preparation for the tissue embedding is disclosed. The apparatus (1) has a free space (13) for the accommodation of a transport plate (15). The apparatus (1) is surrounded by a housing (5) which defines an upper side (8) and a heating and cooling device (3) that is provided on the upper side (8) of the housing (5), and the heating and cooling device (3) has a reception (23) with at least one chamber (32) for the accommodation of at least one processing container (17). The heating and cooling device (3) is pivotable with respect to the upper side (8) of the housing (5).Type: GrantFiled: March 18, 2002Date of Patent: September 6, 2005Assignee: Leica Mikrosysteme GmbHInventors: Reinhard Lihl, Michael Zimmerman, Guenther Bock, Ian Lamswood
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Patent number: 6924114Abstract: The amount of platelet surface proteins in a sample may be measured by collecting a sample containing platelets into a collection tube containing a platelet stabilizing composition, labeling the platelet surface protein and detecting by cytometry.Type: GrantFiled: September 20, 2001Date of Patent: August 2, 2005Assignee: SurroMed, Inc.Inventors: Timothy L. Wyant, Paul A. Raju, Aaron B. Kantor
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Patent number: 6913932Abstract: A stabilization reagent composition, particularly desirable for stabilizing blood samples containing platelets, contains reactants that generate multiple species of formaldehyde-ammonium complexes; at least one inhibitor of phosphatase enzymatic activity; and at least one inhibitor of protease enzymatic activity. Blood samples and other tissues are stabilized by this composition. Such stabilized samples are produced by methods for stabilizing the tissue by contacting the sample with the composition.Type: GrantFiled: August 23, 2002Date of Patent: July 5, 2005Assignee: Beckman Coulter, Inc.Inventors: John A. Maples, Lori A. Charie, Daniel James Flagler, Rhonda Ann Mills, Richard Timmons
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Patent number: 6911313Abstract: A method for discriminating and counting erythroblasts comprises the steps of: (i) staining leukocytes in a hematologic sample by adding a fluorescent labeled antibody capable of binding specifically with leukocytes to the hematologic sample; (ii) raising the permeability only of cell membranes of erythroblasts in the hematologic sample to a nucleotide fluorescent dye which does not permeate a cell membrane usually, the nucleotide fluorescent dye having a fluorescent spectrum capable of being distinguished from that of a fluorescent labeling compound of the fluorescent labeled antibody in step (i); (iii) staining nuclei of the erythroblasts in the hematologic sample with the nucleotide fluorescent dye; (iv) subjecting the hematologic sample to flowcytometry to detect at least two fluorescent signals from each cell; and (v) discriminating and counting the erythroblasts from difference in intensity between the at least two fluorescent signals.Type: GrantFiled: April 9, 1998Date of Patent: June 28, 2005Assignee: Sysmex CorporationInventors: Berend Houwen, Fu-sheng Wang, Tomohiro Tsuji, Takashi Sakata, Yukio Hamaguchi
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Patent number: 6867002Abstract: A sample solution treating instrument is provided for facilitating rapid and simplified adjustment of the condition of a sample solution proper for analysis with a biosensor before supplying the solution to the biosensor. The sample solution treating instrument includes, for example, a catalyst or an adsorbent which can remove any interfering substance in order to adjust the sample solution for measurement with a biosensor.Type: GrantFiled: October 20, 1999Date of Patent: March 15, 2005Assignee: Matsushita Electric Industrial Co., Ltd.Inventors: Mariko Miyashita, Toshihiko Yoshioka, Shiro Nankai
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Patent number: 6864095Abstract: The invention concerns the production of a chemical sensor which can be used to measure nitric acidity. This sensor is produced using a sol-gel method for depositing a porous film, containing a colored indicator, on the core of an optic fiber. The pH of the initial sol is adjusted as are other conditions for implementing the sol-gel method to obtain stability of the signal (curves 1 to 4) emitted by the sensor in an 8N nitric medium for at least 1000 hours.Type: GrantFiled: August 21, 2001Date of Patent: March 8, 2005Assignees: Commissariat a l'Energie Atomique, Compagnie Generale des Matieres NucleairesInventors: Marie-Hélène Noire, Christophe Bouzon, Thierry Davin