Liberation Or Purification Of Sample Or Separation Of Material From A Sample (e.g., Filtering, Centrifuging, Etc.) Patents (Class 436/177)
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Patent number: 8257651Abstract: In one embodiment the present invention provides a blood analyte meter that is user-friendly and easy to use. In accordance with an embodiment of the present invention an analyte measurement device, for use with a test strip for determining the amount of an analyte in a sample, displays a hierarchy of information or options to a user. The hierarchy of information or options may include, among other information or options, subroutines that are performable by the processor of the device, stored data related to past tests performed by the user, and alarm features of the device. A user scrolls through and selects individual options or pieces of information by rotating and translating a rotatable user interface around and along an axis of rotation.Type: GrantFiled: March 16, 2006Date of Patent: September 4, 2012Assignee: AgaMatrix, Inc.Inventors: Joseph Flaherty, Timothy Golnik
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Patent number: 8247235Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.Type: GrantFiled: June 8, 2007Date of Patent: August 21, 2012Assignee: Quest Diagnostics Investments IncorporatedInventors: Michael P. Caulfield, Richard E. Reitz, Shuguang Li, Gloria Kwangja Lee, Ronald Krauss, Patricia J. Blanche, W. Henry Benner, Earl Cornell
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Patent number: 8247234Abstract: Compounds having stable isotopes 13C and/or 2H were synthesized from precursor compositions having solid phase supports or affinity tags.Type: GrantFiled: June 2, 2011Date of Patent: August 21, 2012Assignee: Los Alamos National Security, LLCInventors: Jurgen G. Schmidt, David B. Kimball, Marc A. Alvarez, Robert F. Williams, Rudolfo A. Martinez
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Patent number: 8241592Abstract: Disclosed herein are apparatus and methods for isolating a fraction of interest from a physiological fluid sample. A sample holder for isolating a fraction of interest from a physiological fluid sample includes a flexible compartment and a rigid exoskeleton that supports the flexible compartment. The flexible compartment may have at least one reservoir with a height to volume ratio of about 0.1 cm/mL to about 5 cm/mL. An automated device for extracting a fraction of interest from the sample includes a sample holder with a flexible compartment supported by a rigid exoskeleton, a support for the sample holder connected to one or more fluid extraction devices, and a motor for moving the extraction device relative to the sample holder. The automated device may include an optical sensor and may include a clamp for clamping the flexible compartment.Type: GrantFiled: September 29, 2011Date of Patent: August 14, 2012Assignee: Endocellutions, Inc.Inventors: Neil F. Duffy, Jr., Jeffrey R. Chabot, Edward H. Kislauskis
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Patent number: 8241919Abstract: A method includes acquiring a chemical sample and modulating the chemical sample at a frequency greater than a drift frequency of a sensor. The method also includes determining at least one of a presence and a concentration of the analyte within the modulated chemical sample using the sensor. Modulating the chemical sample could include alternately absorbing at least some of the analyte into a sorbent material and releasing at least some of the analyte from the sorbent material. Modulating the chemical sample could also include heating the sorbent material, absorbing part of the analyte into the sorbent material, and passing a remaining portion of the analyte into the sensor. Modulating the chemical sample could further include stopping the heating of the sorbent material, releasing the part of the analyte from the sorbent material, and passing the sample with the released part of the analyte into the sensor.Type: GrantFiled: October 28, 2008Date of Patent: August 14, 2012Assignee: Honeywell International Inc.Inventors: Adam Dewey McBrady, J. David Zook, Alex Gu, Michael L. Rhodes
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Publication number: 20120202702Abstract: Provided are methods of preparing a sample for detection by placing the sample on a shrinkable scaffold and then shrinking the scaffold. An exemplary shrinkable scaffold is a thermoplastic substrate.Type: ApplicationFiled: February 6, 2012Publication date: August 9, 2012Inventors: Michelle Khine, Himanshu Sharma, Sophia Lin, Jolie McLane
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Publication number: 20120202193Abstract: The invention relates to a disposable device, in which the target substances are bound to solid carriers, preferably microparticles. A mechanism according to the invention enables the solid carriers to be easily concentrated to a volume of appropriate size. A further device according to the invention makes it possible for the solid carriers in the device to be subjected to known isolating, amplifying and/or detection reactions. The device is especially suitable for the enrichment and isolation and/or detection of substances in, or extracted from, biological liquids.Type: ApplicationFiled: August 30, 2010Publication date: August 9, 2012Inventor: Hans-Werner Heinrich
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Patent number: 8232106Abstract: Disclosed are a method for detecting proteins on a polyacrylamide gel by background staining method using an organic dye composition containing eosin Y or phloxine B, and the organic dye composition for use in the method, which enables the rapid and simple detection of the protein on the polyacrylamide gel with a high sensitivity.Type: GrantFiled: October 27, 2008Date of Patent: July 31, 2012Assignee: Industry Foundation of Chonnam National UniversityInventor: Jung-kap Choi
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Compact disk based system for separating immunomagnetic bead labeled particulates and method thereof
Patent number: 8226826Abstract: Disclosed is a disk based system for separating at least two types of particulates contained in a sample fluid. The system includes a disk-like carrier board and a magnetic attraction unit. The disk-like carrier board forms at least one flow channel structure, which includes an inner reservoir, at least one separation chamber, and at least one outer reservoir arranged in sequence from a geometric center of the disk-like carrier board to an outer circumferential rim of the disk-like carrier board. A method of separation carried out with the system includes introducing the sample fluid into the inner reservoir and then rotating the disk-like carrier board to induce a centrifugal force. The sample fluid contains particulates that are labeled with immunomagnetic beads and the labeled particulates are attracted by the magnetic force generated by the magnetic attraction unit to retain in the inner reservoir or the separation chamber.Type: GrantFiled: June 25, 2009Date of Patent: July 24, 2012Assignee: National Taiwan UniversityInventors: Andrew M. Wo, Chen-Lin Chen, Ken-Chao Chen, Yu-Cheng Pan -
Patent number: 8221701Abstract: Provided is a microfluidic device that can automatically perform various types of biological blood analysis. In the microfluidic device, a specimen is centrifugally separated and the centrifugally separated specimen is diluted into various dilution ratios. Also, at least two reagents that are required for one reaction and that need to be separately stored are stored in separate chambers, and they are mixed when a reaction is needed. Thus, various conventional blood analyzing reagents can be used as they are or after being minimally processed in the microfluidic device.Type: GrantFiled: July 30, 2010Date of Patent: July 17, 2012Assignee: Samsung Electronics Co., Ltd.Inventors: Yoon-kyoung Cho, Do-gyoon Kim, Jung-nam Lee, Hee-kyun Lim
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Publication number: 20120178178Abstract: A biosensor cartridge includes a plasma separator, a plasma metering chamber, a mixer, and a biosensor.Type: ApplicationFiled: October 11, 2011Publication date: July 12, 2012Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Youn Suk CHOI, Hun Joo LEE, Jae Phil DO, Soo Suk LEE, Yeol Ho LEE, Jung Nam LEE, Joon Hyung LEE, Kyung Yeon HAN, Sang Kyu KIM
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Publication number: 20120171712Abstract: A process for realising a device (1) for collecting and transferring samples for molecular biology, the device including at least: a support body (2) having at least a first portion (2a), and a plurality of fibres (6) attached and arranged on the first portion (2a) of the support body (2) by flocking, such as to define a flocked collecting portion (3) destined to collect, on the said collecting portion, (3) a quantity of the sample for molecular biology, the process including at least a step of pre-treating the plurality of fibres (6) with a surfactant, the step of pre-treating the plurality of fibres (6) with a surfactant being performed after application of the fibres on the support body (2) by flocking and/or before application of the fibres (6) on the body (2) by flocking and/or wherein the surfactant is added to the fibre (6) during production of the fibre (6).Type: ApplicationFiled: March 8, 2011Publication date: July 5, 2012Inventor: Daniele Triva
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Patent number: 8211710Abstract: A method for obtaining the contents of a fluid-holding vessel having a cap secured to an open end thereof. The method includes penetrating a surface of the cap with a fluid transfer device and forming one or more air passageways between the penetrated surface of the cap and the fluid transfer device to allow air to be vented from the vessel. At least a portion of the contents of the vessel is then drawn into the fluid transfer device before the fluid transfer device is removed from the vessel.Type: GrantFiled: October 30, 2007Date of Patent: July 3, 2012Inventors: Kathleen A. Dickey, Bruce W. Anderson, Mordi I. Iheme, Nick M. Carter, Daniel L. Kacian
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Patent number: 8211715Abstract: A consumer food testing device for testing for the presence of harmful contaminants in a food sample, includes a system for producing a visual cue upon detection of a harmful chemical, biological, and/or ionizing radiation contaminant; and a processing system responsive to the detection of a harmful contaminant, for transmitting the global position of the consumer testing device, the identity of the harmful contaminant, and the time and date to a remote monitoring facility.Type: GrantFiled: November 15, 2011Date of Patent: July 3, 2012Assignee: Harrogate Holdings, Ltd. Co.Inventor: Robert B. Royds
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Publication number: 20120164749Abstract: Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.Type: ApplicationFiled: August 19, 2008Publication date: June 28, 2012Inventors: Alessandra Luchini, Lance Liotta, Emanuel Petricoin, Barney Bishop, Caterina Longo, Virginia Espina, Alexis Patanarut
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Publication number: 20120164743Abstract: A microchannel chip having a microchannel formed in a substrate and a gas-liquid phase separation microchannel whose upper part is covered with a porous film, the gas-liquid phase separation microchannel being connected to the downstream end of the microchannel and having a depth of 10 ?m to 100 ?m. Also, a gas-liquid phase separation method which is a method for separating a liquid-phase flow from a two-phase flow flowing through a microchannel by removing a gas phase, the two-phase flow composed of the gas phase and the liquid phase, which liquid phase flows in the periphery of the above-described microchannel and which gas phase flows interiorly of the liquid-phase flow.Type: ApplicationFiled: March 31, 2010Publication date: June 28, 2012Applicants: INSTITUTE OF MICROCHEMICAL TECHNOLOGY CO., LTD., KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY, THE UNIVERSITY OF TOKYOInventors: Arata Aota, Yuko Kihira, Mari Sasaki, Takehiko Kitamori, Kazuma Mawatari
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Patent number: 8206975Abstract: A method and a microfluidic device are provided to regulate fluid flow by equalization of channel pressures. The fluid flow is regulated by way of valve-actuated channel pressures.Type: GrantFiled: October 27, 2006Date of Patent: June 26, 2012Assignee: California Institute of TechnologyInventors: Xiaoyan Robert Bao, Stephen R. Quake, Melvin I. Simon
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Publication number: 20120149035Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.Type: ApplicationFiled: December 14, 2011Publication date: June 14, 2012Inventors: Tammy Burd, Ian Gibbons, Elizabeth A. Holmes, Gary Frenzel, Anthony Joseph Nugent
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Publication number: 20120149127Abstract: The present invention is to present a sample analyzer for analyzing a target substance in a sample by carrying out a target substance separating process for separating a complex containing the target substance and a magnetic particle from other substances other than the complex. The sample analyzer executes the target substance separating process with respect to a second container held by a second holder with a second nozzle while executing the target substance separating process with respect to a first container held by a first holder with a first nozzle, and completes the target substance separating process with respect to the first container with the first nozzle and completes the target substance separating process with respect to the second container with the second nozzle.Type: ApplicationFiled: December 9, 2011Publication date: June 14, 2012Inventors: Hiroto Toyoshima, Kazunori Mototsu
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Patent number: 8192630Abstract: Methods for in-line purification of surfactant from a first fluid, such as a microemulsion are disclosed. Magnetic particles coated with surfactant molecules may be used to bind surfactants from a fluid. A magnetic field may be used to separate the bound materials from the fluid.Type: GrantFiled: December 21, 2010Date of Patent: June 5, 2012Assignee: Empire Technology Development LLCInventor: Angele Sjong
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Publication number: 20120135541Abstract: Microfluidic devices and methods for using the same are provided. Aspects of the invention include microfluidic devices that include a separation medium and a pan-capture binding medium. The microfluidic devices are configured to subject a sample to two or more directionally distinct electric fields. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.Type: ApplicationFiled: November 22, 2011Publication date: May 31, 2012Inventors: Amy E. Herr, Dohyun Kim
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Patent number: 8187888Abstract: The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.Type: GrantFiled: July 8, 2011Date of Patent: May 29, 2012Assignee: Accuri Cytometers, Inc.Inventor: Collin A. Rich
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Patent number: 8187886Abstract: The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.Type: GrantFiled: January 10, 2011Date of Patent: May 29, 2012Assignee: The General Hospital CorporationInventors: Denise L. Faustman, Douglas E. Burger
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Publication number: 20120129156Abstract: The present invention relates to systems, devices, and methods for performing biological reactions. In particular, the present invention relates to the use of lipophilic, water immiscible, or hydrophobic barriers in sample separation, purification, modification, and analysis processes.Type: ApplicationFiled: December 30, 2011Publication date: May 24, 2012Applicant: NORTHWESTERN UNIVERSITYInventors: David M. Kelso, Kunal Sur, Zaheer Parpia
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Patent number: 8182769Abstract: A transportation system for transporting a biological material container between a sterile field and a nonsterile field and substantially maintaining sterility of the biological material container includes a housing assembly that removably houses the biological material container. The system also includes a port defined by the housing assembly, and the port provides communication into the biological material container from outside the housing assembly. The housing assembly includes a first member that covers a first portion of the biological material container such that a second portion of the biological material container extends from the first member. The housing assembly also includes a second member that covers the second portion of the biological material container. The second member is removably coupled to the first member to expose the second portion of the biological material container. A keying member that keys the transportation system in a centrifuge is also disclosed.Type: GrantFiled: April 4, 2008Date of Patent: May 22, 2012Assignee: Biomet Biologics, LLCInventor: Jason Chavarria
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Patent number: 8183053Abstract: The present invention provides improved preconcentrator media suitable for selectively desorbing captured analytes. Additionally, the current invention provides improved methods for concentrating trace quantities of analytes to a degree suitable for detection by a detecting device.Type: GrantFiled: April 26, 2010Date of Patent: May 22, 2012Assignee: FLIR Systems, Inc.Inventors: Edward Thomas Knobbe, Mark Fisher, Gregory Charles Frye-Mason
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Patent number: 8182768Abstract: A system for interfacing a sampling device and a chromatograph and for pre-concentrating analytes in a sample prior to introducing the sample into the chromatographic column is generally disclosed comprising an interface housing with a first channel and an adsorbent housing with a second channel, which contains at least one adsorbent. Valveless conduits permit fluid to be communicated between the sampling device and the first channel, between the first channel and the second channel, and the first channel and the column. In some embodiments, fluid flows in one direction when the analytes are adsorbed and in the opposite direction when analytes are desorbed. In certain embodiments, two different adsorbents are disposed in the second channel to adsorb different types of analytes.Type: GrantFiled: October 14, 2005Date of Patent: May 22, 2012Assignee: PerkinElmer LAS, Inc.Inventors: Andrew Tipler, John H. Vanderhoef, James E. Botelho
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Patent number: 8178057Abstract: An apparatus for filling a sample volume defining device for separating at least one small defined volume of a liquid sample from a relatively larger undefined volume of said sample, said device including a first body and a second body movable relative to each other, whereby said first body has at least one cavity in a surface thereof, said at least one cavity having said defined volume. One of said first or said second body has at least one inlet opening adapted to be placed in a drop of the liquid sample. A defined channel is provided between said first and second body, which channel has fluid connection with said at least one opening and at least beyond said at least one cavity whereby the dimensions of said channel being such that said channel and said at least one cavity is filled with said liquid sample.Type: GrantFiled: May 18, 2010Date of Patent: May 15, 2012Assignee: Boule Medical ABInventors: Ingemar Berndtsson, Lennart Niklason
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Patent number: 8178058Abstract: In the present invention cells are placed in a multiwell plate and grown. When the assay is to be performed, one uses gravity to wash away any unbound ligands rather than vacuum or centrifugation. The cells are then examined to detect the bound ligand. To perform the washing step(s) the plate is placed into a carrier plate having open wells in register with the wells of the filter plate or one may use a wicking device or an underdrain attached to the bottom of the filter plate. Sufficient wash liquid is added to allow for filtration by the effect of gravity to occur. Cells are retained within the wells at a rate of 4 times that of other rapid methods.Type: GrantFiled: April 12, 2011Date of Patent: May 15, 2012Assignee: EMD Millipore CorporationInventor: Jun Y. Park
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Publication number: 20120115243Abstract: The invention relates to a sample port, filter, and sampling method. The sample port according to the invention comprises a body equipped with an internal cavity and two plungers arranged moveably in this, which can be pressed against each other in the internal cavity, in order to compress a sample, and at least one of which plungers can be moved into a reactor, in order to collect a sample. The sample port also comprises a sample chamber, which is formed by the space remaining between the internal cavity and the plungers, and at least one sample-container connection connected in connection with the internal cavity, in order to collect the sample from sample chamber. In addition, the sample port has filter means adapted to at least either plunger for separating the liquid component of the sample from the solid component and means for leading the liquid component of the sample out of the sample chamber.Type: ApplicationFiled: May 21, 2010Publication date: May 10, 2012Applicant: Teknologian Tutkimuskeskus VTTInventors: Juha-Pekka Pitkänen, Ari Hokkanen, Päivi Heimala, Jari Kauhaniemi, Kai Kolari, Pekka Savolahti, Jaana Uusitalo
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Publication number: 20120107830Abstract: The present disclosure provides isotopically substituted compounds of the formula (II): wherein T, U, V, W, X, Y, Z, R0, R3, R4, R5 and R6 are as defined in the detailed description. The method for detection and quantification using the same is also disclosed.Type: ApplicationFiled: January 9, 2012Publication date: May 3, 2012Inventors: Vincent Jo Davisson, Shirshendu K. Deb, Giselle Marcelline Knudsen-Mooney, Meiguo Xin
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Patent number: 8168440Abstract: The present invention relates generally to methods for enantiomeric separation of complex chemical mixtures using micelles of surfactant molecules that have a sulfate or sulfonate head group, a chiral selector, a linker, and a hydrophobic tail. Also provided are micelle compositions with sulfate or sulfonated head groups, methods of manufacture, and applications thereof. In particular, the micelles of the present invention provide an efficient enantiomeric separation and detection techniques for use in capillary electrophoresis and capillary electrophoresis with mass spectrometry.Type: GrantFiled: March 28, 2007Date of Patent: May 1, 2012Assignee: Georgia State UniversityInventors: Shahab Ahmed Shamsi, Syed Asad Ali Rizvi
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Patent number: 8168442Abstract: A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.Type: GrantFiled: September 29, 2004Date of Patent: May 1, 2012Assignee: CepheidInventors: Kurt E. Petersen, William A. McMillan, Farzad Pourahmadi, Ronald Chang, Douglas B. Dority
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Patent number: 8163183Abstract: To provide a magnetic particle parallel processing apparatus permitting repeated use of a container, and a method of magnetic particle parallel processing permitting repeated use of a container, with which the rate of repeated use of a container is enhanced to thereby achieve a saving in the working space and a saving in the working time. The apparatus comprises: at least one reaction container; a liquid disposal tank; a reagent etc.Type: GrantFiled: February 6, 2008Date of Patent: April 24, 2012Assignee: Universal Bio Research Co., Ltd.Inventor: Hideji Tajima
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Publication number: 20120083046Abstract: The present invention provides a hybrid digital and channel microfluidic device in the form of an integrated structure in which a droplet may be transported by a digital microfluidic array and transferred to a microfluidic channel. In one aspect of the invention, a hybrid device comprises a first substrate having a digital microfluidic array capable of transporting a droplet to a transfer location, and a second substrate having a microfluidic channel. The first and second substrates are affixed to form a hybrid device in which an opening in the microfluidic channel is positioned adjacent to the transfer location, so that a droplet transported to the transfer location contacts the channel opening and may enter the channel. The invention also provides methods of performing separations using a hybrid digital and channel microfluidic device and methods of assembling a hybrid digital microfluidic device.Type: ApplicationFiled: October 13, 2009Publication date: April 5, 2012Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTOInventors: Michael W. L. Watson, Mohamed Abdelgawad, Mais Jebrail, Hao Yang, Aaron R. Wheeler
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Patent number: 8147757Abstract: The present invention describes a system and method for accurately measuring the concentration of a substance within a filter housing. A concentration sensor and a communications device are coupled so as to be able to measure and transmit the concentration of a particular substance within the filter housing while in use. This system can comprise a single component, integrating both the communication device and the concentration sensor. Alternatively, the system can comprise separate sensor and transmitter components, in communication with one another. In yet another embodiment, a storage element can be added to the system, thereby allowing the device to store a set of concentration values. The use of this device is beneficial to many applications. For example, the ability to read concentration values in situ allows integrity tests to be performed without additional equipment.Type: GrantFiled: December 16, 2010Date of Patent: April 3, 2012Assignee: EMD Millipore CorporationInventor: Anthony DiLeo
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Patent number: 8148168Abstract: The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.Type: GrantFiled: July 15, 2010Date of Patent: April 3, 2012Assignee: PhyNexus, Inc.Inventors: Douglas T. Gjerde, Allen Burge, Ronald Jones, Mark Abel
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Publication number: 20120070833Abstract: Provided herein is a microfluidic device and related method for controlling flow of different fluid components of a fluid. The microfluidic device comprises an input channel, focusing channel and an assaying channel. The microfluidic device is adapted to separate a fluid into at least two fluid components, and is further adapted to detect a target material comprised within one of the fluid components. The method comprises providing a channel, the channel having a dimension which is a function of a dimension of one of the fluid components and deliver the fluid through the channel at a set flow rate.Type: ApplicationFiled: September 22, 2011Publication date: March 22, 2012Inventors: Jun WANG, James R. HEATH
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Patent number: 8137979Abstract: Improved preconcentrators, particularly MEMs scale preconcentrators which possess a coating comprising polymers of intrinsic microporosity (PIMs). There is further provided devices comprising the preconcentrator, and methods of preparation and use. There is particular benefit directed to the use of a MEMs scale heater coated with the PIMs for use in hand-held or field portable chemical detection devices. The polymer of intrinsic microporosity comprises a polymer, with a monomer repeat unit of Formula I wherein A is one or more optionally substituted aryl, heterocyclic, cycloalkyl or bicycloalkyl rings, ? is greater than 5, preferably 5 to 10000, and X may be selected from CH, CH2, O, S, N or NH.Type: GrantFiled: June 25, 2008Date of Patent: March 20, 2012Assignee: QinetiQ LimitedInventors: David J. Combes, Timothy I Cox, Ian C Sage
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Patent number: 8137983Abstract: The present invention describes a system and method for accurately measuring the concentration of a substance within a filter housing. A concentration sensor and a communications device are coupled so as to be able to measure and transmit the concentration of a particular substance within the filter housing while in use. This system can comprise a single component, integrating both the communication device and the concentration sensor. Alternatively, the system can comprise separate sensor and transmitter components, in communication with one another. In yet another embodiment, a storage element can be added to the system, thereby allowing the device to store a set of concentration values. The use of this device is beneficial to many applications. For example, the ability to read concentration values in situ allows integrity tests to be performed without additional equipment.Type: GrantFiled: October 17, 2011Date of Patent: March 20, 2012Assignee: EMD Millipore CorporationInventor: Anthony DiLeo
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Patent number: 8133691Abstract: The invention relates to a kit comprising MHC Class I and Class II HLA-coated beads containing specific antigenic peptides for binding to antigen-specific T cells and the appropriate negative control peptides. Also provided are methods for making the coated beads and methods for use. The application of these beads go to the stimulation of peripheral blood cell populations and in vitro-stimulated culture for the elicitation of functional activities such as cell activation and signaling, cytokine secretion, proliferation and cytotoxicity activity.Type: GrantFiled: July 5, 2006Date of Patent: March 13, 2012Assignee: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.Inventors: Sathibalan Ponniah, George E. Peoples, Catherine E. Storrer, Michael Flora
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Patent number: 8124028Abstract: The present invention provides a novel system for efficiently and accurately analyzing targets in samples and for preparing samples for analysis with various analytical methods. A version of the present invention comprises a multi-function probe, configured for pipetting liquids directly, with a pipet tip, or with an assay unit described herein. Another version of the present invention includes an apparatus for conducting an immunoassay or selective adsorption separation comprising an assay unit and a multi-function probe. Another version of the present invention includes a multi-function probe and assay unit for use with an automated Cartesian robot. Other versions include one- or two-dimensional arrays comprising multi-function probes connected to syringe barrels for use with assay units.Type: GrantFiled: December 19, 2008Date of Patent: February 28, 2012Assignee: Agilent Technologies, Inc.Inventors: Scott P. Fulton, Robert J. Sakowski
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Patent number: 8114680Abstract: A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.Type: GrantFiled: March 21, 2011Date of Patent: February 14, 2012Assignee: Battelle Memorial InstituteInventors: Thomas Haubert, Vince Contini, Steve Grimes, Randy Jones, Stephen C. Wardlaw
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Patent number: 8110125Abstract: The separation of single-walled carbon nanotubes (SWNTs), by chirality and/or diameter, using centrifugation of compositions of SWNTs in and surface active components in density gradient media.Type: GrantFiled: January 5, 2010Date of Patent: February 7, 2012Assignee: Northwestern UniversityInventors: Mark C. Hersam, Samuel I. Stupp, Michael S. Arnold
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Patent number: 8110403Abstract: It is an object of the present invention to provide an immunoassay method which is capable of simultaneous quantification of a plurality of test substances under a same analysis condition, through adjustment of the measurement sensitivity/range by simply varying the size of particles for use as labels, without changing the spectra of these particles.Type: GrantFiled: February 11, 2009Date of Patent: February 7, 2012Assignee: Fujifilm CorporationInventors: Tadahiro Matsuno, Kentaro Nakamura
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Patent number: 8101138Abstract: A centrifugal micro fluidic system and a centrifugal magnetic position control device used in the centrifugal micro fluidic system for controlling the position of magnetic beads are provided. The centrifugal micro fluidic system comprising, a rotatable platform; a micro fluidic structure which is disposed in the platform; and a plurality of objects which include functional groups on surfaces thereof so as to capture a target material from the fluid and carry the target material while being suspended in and separated from the fluid in the micro fluidic structure, wherein the movements of the objects are controlled by a force affecting the objects differently compared to the force's effect on the fluid. When the objects are made of a magnetic material, the force may be a magnetic force applied by the centrifugal magnetic position control device.Type: GrantFiled: August 10, 2007Date of Patent: January 24, 2012Assignee: Samsung Electronics Co., Ltd.Inventors: Jeong-gun Lee, Yoon-kyoung Cho, Beom-seok Lee, Jong-myeon Park
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Patent number: 8093015Abstract: The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.Type: GrantFiled: April 24, 2007Date of Patent: January 10, 2012Assignee: Sartorius Stedim Biotech GmbHInventors: Stefan Obermann, Reinhard Baumfalk, Oscar-Werner Reif, Florian Wurm, Maria de Jesus, Matthieu Stettler, Martin Jordan
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Patent number: 8088630Abstract: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.Type: GrantFiled: September 25, 2009Date of Patent: January 3, 2012Inventors: Paul V. Lehmann, Alexey Y. Karulin, Thomas Oliver Kleen
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Patent number: 8088625Abstract: An object of the present invention is to provide an excellent method for detection and diagnosis of familial combined hyperlipidemia. The present invention relates to a method for detection of familial combined hyperlipidemia by measuring the concentration of small, dense LDL cholesterol in a sample collected from a subject.Type: GrantFiled: May 1, 2007Date of Patent: January 3, 2012Assignee: Denka Seiken Co., Ltd.Inventor: Yasuki Itoh
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Patent number: 8084223Abstract: Methods and reagents are disclosed for detecting a false result in an assay for determining a concentration of an analyte in a whole blood sample suspected of containing the analyte. The method comprises determining by means of the assay a concentration of the analyte utilizing a hemolyzed portion of the blood sample to obtain concentration value 1 and determining by means of the assay a concentration of the analyte utilizing a non-hemolyzed portion of the blood sample and multiplying the concentration times a hematocrit factor to obtain concentration value 2. A ratio of concentration value 1 divided by concentration value 2 is determined and is compared to a predetermined ratio of known reliability. If the ratio is less than the predetermined ratio, a false result is indicated.Type: GrantFiled: March 23, 2009Date of Patent: December 27, 2011Assignee: Siemens Healthcare Diagnostics Inc.Inventor: Tie Quan Wei