Preservative, Buffer, Anticoagulant Or Diluent Patents (Class 436/18)
  • Patent number: 10321861
    Abstract: The present invention relates to a pH sensor adapted to be inserted into all soft tissues, such as muscle, fat or other organs e.g. heart, lung, kidney, liver, pancreas, renal gland etc., comprising one or more of: a) means to provide sensor calibration performance information; b) a sensor dislodgement alert, providing an indication to the user if the pH reading falls outside a predetermined range; c) an alert to indicate that the sensor has exceeded its usage period; and d) an alert to indicate that the sensor had exceeded its shelf life. There is also provided a method of assessing the efficacy of a treatment regime comprising the steps of determining any change in the pH of the soft tissue during treatment, where any change in the pH towards the preferred range for the tissue type is indicative of an effective treatment regime and any change in the pH away from the preferred range for the tissue type is indicative of a worsening in the condition of the tissue, and thus an ineffective treatment regime.
    Type: Grant
    Filed: June 11, 2013
    Date of Patent: June 18, 2019
    Assignee: SOFTCELL MEDICAL LIMITED
    Inventor: Kirsty McIntosh
  • Patent number: 9551022
    Abstract: Provided is a method for measuring a substance in a blood sample, which allows avoidance of the influences of both of bilirubin and hemoglobin by simple operations. Provided is a method for measuring a substance in a blood sample by an enzymatic method using an oxidizable color reagent, the method including (1) bringing the blood sample into contact with a non-ionic surfactant; and then (2) bringing the resultant sample into contact with a betaine-type amphoteric surfactant, to perform an enzyme reaction and a color reaction by an oxidizable color reagent at the same time as the contact or after the contact.
    Type: Grant
    Filed: April 1, 2013
    Date of Patent: January 24, 2017
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventor: Toyomi Yamaguchi
  • Patent number: 9128105
    Abstract: A synthetic urine solution and method of its manufacture are disclosed. The solution includes water having a pH between about 3 and about 10. The solution further includes creatinine, a means for removing bacteria from the solution so as to control or eliminate sepsis of the urine solution, preferably through the use of a biocide, and a urea-based compound. The solution exhibits a specific gravity of from 1.005 g/cm3 to 1.025 g/cm3. Additional compounds may also be included to further enhance the aesthetics or apparent authenticity of the synthetic urine.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: September 8, 2015
    Assignee: Spectrum Laboratories, LLC
    Inventor: James Matthew Stephens
  • Patent number: 9029154
    Abstract: A biological reaction apparatus for receiving at least one substrate having a sample located in a sample region, and a separate cover, such that a reaction chamber is formed between the cover and substrate over the sample region. The apparatus includes a substrate locator for locating the substrate; a cover locator for locating and moving the cover with respect to the substrate; a fluid dispenser for dispensing fluid into the reaction chamber; and a draining mechanism which includes a wick.
    Type: Grant
    Filed: February 1, 2010
    Date of Patent: May 12, 2015
    Assignee: Leica Biosystems Melbourne Pty Ltd
    Inventors: Stuart Elliot, Andrew McLellan, Chester Henderson, Mark Dockrill, Simon Harris, Peter Riddell
  • Patent number: 9022865
    Abstract: In some embodiments, a player may influence the volatility of a bonus round of a game by placing elements collected during a primary game onto symbol positions of the bonus round. In some embodiments, if a symbol position onto which a player placed one or more bonus round symbols is selected as an active symbol position for an event instance of the bonus round, the player wins a prize. Additionally, a multiplier may be applied to the value of the prize or other benefit may be provided to the player if the player placed more than one bonus round symbol element on the position. Thus, a player having a plurality of bonus round symbols to place may choose to increase the frequency of prizes won (e.g., by distributing the bonus round symbols over more positions) or increase the value of prizes (e.g., by grouping more symbols on fewer positions).
    Type: Grant
    Filed: April 26, 2013
    Date of Patent: May 5, 2015
    Assignee: Gamesys, Ltd.
    Inventors: Hans Elias, Thomas David James Aicken, Marlon James Dublin
  • Patent number: 8945928
    Abstract: The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5? end to prohibit degradation by a 5?-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface.
    Type: Grant
    Filed: June 6, 2013
    Date of Patent: February 3, 2015
    Inventors: Kerry B Gunning, Mark Aaron Behlke
  • Publication number: 20150024502
    Abstract: Methods for stabilizing reference standards used in total organic carbon (TOC) analysis of water and pharmaceutical samples are disclosed. The methods include adding a preservative to TOC reference standards. The preservative is selected from the group consisting of copper (II) sulfate and hydrogen sulfite. Compositions of the stabilized reference standards are also disclosed.
    Type: Application
    Filed: January 9, 2012
    Publication date: January 22, 2015
    Inventor: Shouquan Deng
  • Patent number: 8921113
    Abstract: A buffer kit includes a first eluent and second eluent. The first eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The second eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The first eluent solution has a first pH and the second eluent solution has a second pH where the first pH and second pH are different values. The buffer kit provides a linear pH gradient that forms an approximately straight line from at least the first pH to the second pH.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: December 30, 2014
    Assignee: Dionex Corporation
    Inventors: Shanhua Lin, Christopher A. Pohl
  • Patent number: 8877505
    Abstract: Embodiments of a composition for stabilizing fluorescent signal of nanoparticles and methods for its use are disclosed. In some embodiments, the composition has a pH from 7 to 10 and includes borate, protein and/or protein hydrolysate, an amine, a preservative, and a nonionic surfactant. In particular embodiments, the amine is an N-ethanol substituted amine, such as ethanolamine, diethanolamine, triethanolamine, N-methyldiethanolamine, N,N-dimethylethanolamine, or a combination thereof. In some embodiments, a fluorescent particle solution, such as a quantum dot solution or quantum dot conjugate solution, is diluted in the composition and stored at 4° C. In certain embodiments, the fluorescence intensity of the diluted fluorescent particle remains substantially the same when stored at 4° C. for at least one month or at least three months. In particular embodiments, a diluted quantum dot conjugate is used to detect a hybridized probe or a protein antigen.
    Type: Grant
    Filed: February 1, 2011
    Date of Patent: November 4, 2014
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Eric May, Alexandra Nagy, Jerome Kosmeder
  • Patent number: 8865416
    Abstract: Analyte sensors, methods for producing and using analyte sensors, methods of detecting and/or measuring analyte activity, detecting pH change, and/or, controlling the concentration of an analyte in a system, are disclosed. Embodiments of the analyte sensors according to the disclosure can provide an accurate and convenient method for characterizing analyte activity, detecting pH change, controlling the concentration of an analyte in a system, and the like, in both in vivo and in vitro environments, in particular in living cell imaging.
    Type: Grant
    Filed: October 19, 2011
    Date of Patent: October 21, 2014
    Assignee: Georgia State University Research Foundation, Inc.
    Inventors: Jenny Jie Yang, Shen Tang
  • Patent number: 8845415
    Abstract: In various embodiments, players around the casino may predict cards to be dealt in a game of blackjack.
    Type: Grant
    Filed: July 6, 2012
    Date of Patent: September 30, 2014
    Assignee: CFPH, LLC
    Inventors: Howard W. Lutnick, Dean P. Alderucci, Geoffrey M. Gelman, Kevin Burman
  • Patent number: 8802385
    Abstract: The present invention relates to a new suspension medium or diluent solution for red blood cells for use in haematological methods. The suspension medium or diluent solution for red blood cells may comprise a combination of two or more amino acids of any group, and preserves the red blood cells in the sample for at least 8 weeks.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: August 12, 2014
    Assignee: Grifols, S.A.
    Inventors: Daniel Martorell Pena, Ma del Carmen Traves Polo, Jordi Farre Leon, Josefina Castells Parera
  • Patent number: 8796028
    Abstract: The invention relates to a method for treating a biological sample, comprising the following method steps: i) preparation of a biological sample and ii) bringing the biological sample into contact with a composition, comprising: (1) 1 to 100 wt. % of at least one polyol and (2) 0 to 99 wt. % of at least one additive, wherein the total amount of components (1) and (2) is 100 wt. %. The invention further relates to biological samples obtained by said method, a method for analysis of a treated biological sample, devices for treating a biological sample, use of said devices, various kits and use of a composition.
    Type: Grant
    Filed: December 29, 2006
    Date of Patent: August 5, 2014
    Assignee: Qiagen GmbH
    Inventor: Vera Holländer
  • Patent number: 8785201
    Abstract: The invention relates to improved electrochemiluminescence assay methods for phosphorylated peptides or proteins employing phospho-specific antibodies and buffer compositions that are substantially free of inorganic phosphate.
    Type: Grant
    Filed: February 10, 2009
    Date of Patent: July 22, 2014
    Assignee: Meso Scale Technologies, LLC
    Inventors: Michael Tsionsky, Eli N. Glezer, Selen Altunata, George Sigal, Jonathan K. Leland, Mark A. Billadeau, Svetlana Leytner, Mark Martin, Larry Helms
  • Publication number: 20140179008
    Abstract: A buffer kit includes a first eluent and second eluent. The first eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The second eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The first eluent solution has a first pH and the second eluent solution has a second pH where the first pH and second pH are different values. The buffer kit provides a linear pH gradient that forms an approximately straight line from at least the first pH to the second pH.
    Type: Application
    Filed: December 21, 2012
    Publication date: June 26, 2014
    Inventors: Shanhua LIN, Christopher A. POHL
  • Patent number: 8753888
    Abstract: The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: June 17, 2014
    Assignee: The General Hospital Corporation
    Inventors: Denise L. Faustman, Douglas E. Burger
  • Patent number: 8753836
    Abstract: Dewaxing buffers containing a water-soluble organic solvent, methods for use thereof, and kits incorporating dewaxing buffers. The dewaxing buffers contain a water soluble organic solvent that has a boiling point of at least 80° C. The dewaxing buffers described herein can be used to quickly and efficiently remove embedding paraffin from slide-mounted tissue sections in an aqueous buffer solution at elevated temperature without fear of paraffin redeposition of the slides. In addition, the dewaxing buffers described herein can be used to perform dewaxing and HIER in a single step, which can substantially reduce protocol time and lower the risk of sample loss or damage.
    Type: Grant
    Filed: January 23, 2012
    Date of Patent: June 17, 2014
    Assignee: Richard-Allan Scientific Company
    Inventors: Jennifer H. Freeland, Marsha A. Harvey
  • Patent number: 8703490
    Abstract: Histochemical process compositions comprising at least one nanoparticle in an amount effective to reduce or substantially eliminate the average number of spots per slide that result from a sample staining protocol are disclosed. Various nanoparticles, or combinations thereof, including metals, metal alloys, metal oxides, ceramics, functionalized metals or metalloids, and other miscellaneous nanoparticles, such as carbon nanoparticles and diamond nanoparticles, can be used. The nanoparticle concentration typically is from about 2 parts per million to about 20 parts per million. One embodiment of a disclosed method concerns applying a histochemical process composition to a sample, followed by performing a staining protocol on the sample. Particular embodiments concern dispensing a nanosolution onto a sample using an automated system, heating the sample, and performing a sample staining process.
    Type: Grant
    Filed: June 1, 2009
    Date of Patent: April 22, 2014
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Merrill Jackson, Michael Herman, Grace Hoben, Noemi Sebastiao, Scott Cockayne, Heather Ferrea
  • Patent number: 8669111
    Abstract: The present invention provides reagents and methods for limiting adsorptive and/or oxidative protein losses in a sample.
    Type: Grant
    Filed: January 24, 2013
    Date of Patent: March 11, 2014
    Assignee: Arizona Board of Regents, A Body Corporate of the State of Arizona Acting for and on Behalf of Arizona State University
    Inventors: Douglas Rehder, Matthew Schaab, Chad Borges
  • Patent number: 8647878
    Abstract: The invention relates to an immersion liquid including at least one compound of the general formula I in which R1 and R2 each include at least one ring structure and X denotes: O, S, NR3 with R3 selected from hydrogen or hydrocarbon, wherein Y denotes independently of each other O, S or NR4 with R4 selected from hydrogen or hydrocarbon. In addition, the invention relates to a further immersion liquid, a compound for an immersion liquid and a use of the immersion liquids or of the compound as an immersion oil for a microscope, in particular for a near-field microscope.
    Type: Grant
    Filed: February 24, 2010
    Date of Patent: February 11, 2014
    Assignee: Carl Zeiss AG
    Inventor: Hans-Joachim Weippert
  • Patent number: 8617895
    Abstract: The present invention is directed to a pre-coated substrate, such as a slide, that is useful for immobilizing a sample. The invention is further provides methods of preparing such pre-coated substrates and methods of analyzing biological samples immobilized on such pre-coated substrate. The substrate is coated with a polycationic polymeric coating material specifically selected such that that coated substrate exhibits increased stability and prolonged shelf-life. Preferred polymeric coating materials include allylic or vinylic polymers having cationic groups thereon and having no more than a small percentage of peptidic monomeric linkages, particularly polydiallyldimethylammonium (PDDA).
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: December 31, 2013
    Assignee: Tripath Imaging, Inc.
    Inventors: William Alan Fox, William Carl Ray, III
  • Patent number: 8597954
    Abstract: The present invention relates to a stool sample processing method and stool sample processing container the a stool sample processing container provided with a stool collection tool, a suspending solution holding portion and a processing solution holding portion, wherein stool sample preparation solutions consisting of a suspending solution and a stool sample processing solution are respectively housed in a suspending solution holding container and a processing solution holding container, after first mixing a stool sample with the suspending solution and suspending therein, a sealant is released into the suspending solution holding container by pressing on the lower portion of the processing solution holding container, and the resulting stool suspension mixes with the stool sample processing solution that stabilizes the nuclide acid.
    Type: Grant
    Filed: November 11, 2010
    Date of Patent: December 3, 2013
    Assignee: Olympus Corporation
    Inventors: Yasuo Tanigami, Kazue Nakajima, Takami Shibazaki
  • Patent number: 8546075
    Abstract: A method of treating samples containing hepatitis C virus (HCV) which method comprises treating HCV-containing samples with a treating agent containing (1) an acidifying agent, and (2) a protein-denaturing agent, or an amphoteric surfactant or a cationic surfactant having both a straight chain alkyl group of 10 or more carbon atoms and a tertiary amine or a quaternary ammonium salt in the same molecule, to effect the release of the HCV antigen and the inactivation of antibodies that bind to the HCV antigen, and the like.
    Type: Grant
    Filed: October 28, 2004
    Date of Patent: October 1, 2013
    Assignee: Advanced Life Science Institute, Inc.
    Inventors: Katsumi Aoyagi, Kumiko Iida, Naoko Matsubara
  • Patent number: 8517967
    Abstract: The present invention provides modified platelets having a reduced platelet clearance and methods for reducing platelet clearance. Also provided are compositions for the preservation of platelets. The invention also provides methods for making a pharmaceutical composition containing the modified platelets and for administering the pharmaceutical composition to a mammal to mediate hemostasis.
    Type: Grant
    Filed: November 8, 2011
    Date of Patent: August 27, 2013
    Assignee: Velico Medical, Inc.
    Inventors: Keith Rosiello, Henrik Clausen
  • Patent number: 8506898
    Abstract: A kit is provided for gathering biological specimens. The kit generally comprises a self-contained specimen gathering device including a protective housing and a preservation solution. Also provided is a method of gathering specimens and a holistic method of deterring crime using the kit.
    Type: Grant
    Filed: July 16, 2008
    Date of Patent: August 13, 2013
    Assignee: Perez Forensic Strategies, LLC
    Inventor: Vincent I. Perez
  • Publication number: 20130189169
    Abstract: The present invention provides reagents and methods for limiting adsorptive and/or oxidative protein losses in a sample.
    Type: Application
    Filed: January 24, 2013
    Publication date: July 25, 2013
    Applicant: Arizona Board of Regents, a body corporate acting for and on behalf of Arizona State University
    Inventor: Arizona Board of Regents, a body corporate acting for and on behalf of Arizona State University
  • Patent number: 8445284
    Abstract: The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The formulations can include a cytological fixative solution including Azure B, a surfactant, methanol, and ethylene glycol. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens.
    Type: Grant
    Filed: June 18, 2012
    Date of Patent: May 21, 2013
    Assignee: Constitution Medical, Inc.
    Inventors: Daniel Lapen, Kerri Licari
  • Patent number: 8438898
    Abstract: Methods and systems for preparation of calibrant infusion fluid sources are disclosed. In one embodiment, a precise volume of glucose is injected into a saline-solution filled calibrant infusion fluid source proximate in time to conducting a calibration procedure. The glucose concentration in the calibrant infusion fluid source is subsequently calculated based on the measured weight of the saline-solution, as determined prior to glucose injection, and the volume of glucose injected. This method provides a highly accurate and convenient manner for use in a hospital environment, for example, with an intravenous blood glucose sensor system. In another embodiment, a premixed calibrant infusion fluid source is provided that includes saline solution and a predetermined concentration of glucose. In such embodiments, shelf life problems related to water evaporation are mitigated by hermetically covering or otherwise hermetically containing the calibrant infusion fluid source up until the point of use.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: May 14, 2013
    Assignee: Edwards Lifesciences Corporation
    Inventors: James R. Petisce, Michael J. Higgins, Kristie Trinh, Todd Abraham
  • Patent number: 8430747
    Abstract: A gaming system including a central server linked to a plurality of gaming machines and a plurality of bonus awards. The central server monitors wagers on the gaming machines. Based at least in part on the wagers the central server determines when bonus events will occur and which gaming machine(s) will provide the bonus awards. In one embodiment, the central server determines which gaming machine will provide a primary bonus award and any secondary bonus awards, wherein at least one gaming machine may be provided a plurality of bonus awards.
    Type: Grant
    Filed: July 30, 2007
    Date of Patent: April 30, 2013
    Assignee: IGT
    Inventors: Carl V. Kniesteadt, Anthony J. Baerlocher, James A. Vasquez, Tonja M. Ferry, Erick T. Ching
  • Patent number: 8377695
    Abstract: A synthetic urine solution is described. The synthetic urine solution is a shelf stable, food grade composition formed of water having a pH between 3 and 10. A thickening agent is dissolved within the water to form a solution having a specific gravity between 1.025 g/cm3 and 1.225 g/cm3. To provide a realistic appearance and odor, a coloring agent and urea are dissolved within the solution. Finally, to provide a shelf stable product, a preservative is also dissolved within the solution.
    Type: Grant
    Filed: April 2, 2012
    Date of Patent: February 19, 2013
    Inventor: Brady Alfred Daniel
  • Patent number: 8357539
    Abstract: An activated partial thromboplastin time measuring reagent, including a first reagent containing a phospholipid and an activator and a second reagent containing a herparin neutralizer and a calcium salt is disclosed. An activated partial thromboplastin time measuring method, and a determination method for determining a presence or absence of a blood coagulation inhibitor are also disclosed.
    Type: Grant
    Filed: December 22, 2010
    Date of Patent: January 22, 2013
    Assignee: Sysmex Corporation
    Inventors: Kazuyo Yoshida, Masahiro Okuda
  • Patent number: 8343769
    Abstract: An immersion solution for a microscope, the immersion solution including a metal-halogeno complex anion containing bromine or iodine and one or more types of metal elements M selected from Sn, In, Bi, Sb, Zn and Al, and an imidazolium cation, a pyridinium cation, a pyrrolidinium cation or an ammonium cation. The immersion solution includes an ionic liquid that transmits light having a predetermined wavelength, has a refractive index of no less than 1.60 and is used for a fluorescence microscope.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: January 1, 2013
    Assignee: Olympus Medical Systems Corp.
    Inventors: Hiroaki Kinoshita, Atsushi Niwa, Masahiro Sato
  • Patent number: 8329943
    Abstract: A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.
    Type: Grant
    Filed: October 6, 2008
    Date of Patent: December 11, 2012
    Assignee: Eiken Chemical Co., Ltd.
    Inventor: Shin Sugo
  • Patent number: 8318418
    Abstract: Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d5 testosterone.
    Type: Grant
    Filed: October 28, 2009
    Date of Patent: November 27, 2012
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Michael P. Caulfield, Darren A Carns, Richard E Reitz
  • Patent number: 8304243
    Abstract: A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.
    Type: Grant
    Filed: March 23, 2012
    Date of Patent: November 6, 2012
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bing Liu, Wenjuan Xu, Xiangping Meng, Yanmei Qiao, Mulong Liu
  • Patent number: 8273580
    Abstract: The invention provides an accurate, economical, automatable, high throughput method for the determination of the concentration of glycosaminoglycan anticoagulants, including low molecular weight heparin (LMWH) anticoagulants, in aqueous solutions. A method for cleaning a unit of manufacturing equipment used in the preparation of a LMWH to obtain an acceptable residual concentration of LMWH is further provided.
    Type: Grant
    Filed: July 5, 2011
    Date of Patent: September 25, 2012
    Assignee: Active BioMaterials, LLC
    Inventors: Ralph Yamamoto, Yumi Kim
  • Patent number: 8232105
    Abstract: Certain embodiments described herein are directed to a reagent that includes an effective amount of an adsorber to remove interfering species present during heavy metal level measurement in a fluid sample. In some examples, the reagent can include an effective amount of an adsorber to remove a suitable amount of glutathione from the fluid sample such that the glutathione does not interfere with measurements of lead levels in the fluid sample.
    Type: Grant
    Filed: September 30, 2010
    Date of Patent: July 31, 2012
    Assignee: Magellan Biosciences Point-of-Care, Inc.
    Inventor: W James Scott
  • Patent number: 8187886
    Abstract: The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
    Type: Grant
    Filed: January 10, 2011
    Date of Patent: May 29, 2012
    Assignee: The General Hospital Corporation
    Inventors: Denise L. Faustman, Douglas E. Burger
  • Patent number: 8183046
    Abstract: A method for preparing a composition that includes selecting a pH of the composition; selecting a first buffer with a negative temperature coefficient; selecting a second buffer with a positive temperature coefficient; and forming the composition comprising the first buffer and the second buffer. The composition has an average temperature coefficient, ?pH/?T(Ta,Tb)?1×10?3 pH-unit/K and a ?pH(Ta,Tb)?0.31 pH-unit for Ta=4 K and Tb=313 K.
    Type: Grant
    Filed: January 11, 2007
    Date of Patent: May 22, 2012
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Yi Lu, Hee-Jung Hwang, Nathan Sieracki, Dewain Garner
  • Patent number: 8163559
    Abstract: A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolality below 50 mOsm/kg H2O. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.
    Type: Grant
    Filed: January 28, 2011
    Date of Patent: April 24, 2012
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Yanmei Qiao, Bing Liu, Xiangping Meng, Wenjuan Xu, Mulong Liu
  • Patent number: 8148156
    Abstract: A synthetic urine solution is described. The synthetic urine solution is a shelf stable, food grade composition formed of water having a pH between 3 and 10. A thickening agent is dissolved within the water to form a solution having a specific gravity between 1.025 g/cm3 and 1.225 g/cm3. To provide a realistic appearance and odor, a coloring agent and urea are dissolved within the solution. Finally, to provide a shelf stable product, a preservative is also dissolved within the solution.
    Type: Grant
    Filed: November 12, 2009
    Date of Patent: April 3, 2012
    Inventor: Brady Alfred Daniel
  • Patent number: 8143064
    Abstract: A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.
    Type: Grant
    Filed: November 5, 2008
    Date of Patent: March 27, 2012
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Bing Liu, Wenjuan Xu, Xiangping Meng, Yanmei Qiao, Mulong Liu
  • Patent number: 8137987
    Abstract: Bodily fluid is analyzed for the presence of drugs of a selected panel of drugs in a simultaneous assay in which sample of the fluid is incubated with additional amounts of all drugs of the panel, antibodies specific to each of the drugs of the panel, and microparticles, the microparticles being divided into subsets, one subset for each drug in the panel and each subset distinguishable from the others. The incubation is performed in a liquid medium in which competitive binding occurs, the drugs in the sample competing with those added to the assay medium for binding to the antibodies. In one procedure, the added drugs are pre-coupled to the microparticles while the antibodies are not, and the incubation is followed by further incubating the microparticles with labeled ligands that have affinity for the antibodies.
    Type: Grant
    Filed: April 13, 2011
    Date of Patent: March 20, 2012
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: William F. Link, Renato B. del Rosario, Randy V. Sweet, David L. King
  • Patent number: 8105840
    Abstract: Described are a urine pretreatment agent, a urine pretreatment method, and a urinary protein quantitation method which reduce or cancel the influences of urine pH variations, cancel the influences of precipitates of urinary inorganic salts, and solubilize membrane proteins. The urine pretreatment agent for urinary protein quantitation, includes a buffer, a chelating agent, and a surfactant; the urine pretreatment method includes a step of mixing 10 to 1000 parts by mass of the urine pretreatment agent of the present invention with 100 parts by mass of urine; and the urinary protein quantitation method includes steps of: mixing 10 to 1000 parts by mass of the urine pretreatment agent with 100 parts by mass of urine; and then measuring the protein concentration.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: January 31, 2012
    Assignees: Niigata University, Denka Seiken Co., Ltd.
    Inventors: Masanori Hara, Akihiko Saito, Shinya Ogasawara, Yoshiaki Hirayama, Hiroyuki Kurosawa
  • Publication number: 20120003743
    Abstract: An object of the present invention is to provide a control material which can be preferably employed in detection of 1,5-anhydro-D-glucitol and glycoalbumin, which are employed as excellent indices for diabetes. The present inventor has found that mannitol which is added to serum or plasma used as control material stabilizes 1,5-anhydro-D-glucitol and glycoalbumin present in the serum or plasma for a long period of time, and that the object can be attained through provision of (1) an agent for stabilizing control material, the agent being composed of mannitol and (2) control material containing mannitol and 1,5-anhydro-D-glucitol.
    Type: Application
    Filed: March 4, 2010
    Publication date: January 5, 2012
    Applicant: BML, INC.
    Inventor: Isami Tsuboi
  • Patent number: 8080423
    Abstract: Stabilizers for colorants used for detection of enzymatic reactions, having a cyclodextrin and a catalase; reagents including stabilizers; and methods of use of such stabilizers and reagents.
    Type: Grant
    Filed: August 4, 2005
    Date of Patent: December 20, 2011
    Assignee: Asahi Kasei Pharma Corporation
    Inventors: Takuji Kouzuma, Yoko Nagai, Shigeyuki Imamura
  • Patent number: 8071317
    Abstract: Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium. The combination comprises (i) the sample, (ii) a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and (iii) a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent comprises a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further comprise a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties.
    Type: Grant
    Filed: February 14, 2011
    Date of Patent: December 6, 2011
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Tie Q. Wei, Alan Craig, Amy Posey
  • Patent number: 8012753
    Abstract: The present invention provides methods for regrowth of conifer embryogenic cells. The methods comprise the steps of (a) contacting cryopreserved conifer embryogenic cells with a liquid transition medium, and (b) culturing the contacted conifer embryogenic cells in a regrowth medium to generate regrowth of the conifer embryogenic cells. Generally, the cryopreserved conifer embryogenic cells are contacted with the liquid transition medium for less than about 24 hours, such as for about one hour.
    Type: Grant
    Filed: June 24, 2004
    Date of Patent: September 6, 2011
    Assignee: Weyerhaeuser NR Company
    Inventors: Pramod K. Gupta, Bonnie Larson, Doris Budworth
  • Patent number: 8003768
    Abstract: A system and method for providing 20% ethanol solutions meeting bioburden and endotoxin specifications, which provides the 20% ethanol solution in a ready-to-use form which may be dispensed directly from the container in which the solution is shipped, and which may be used in connection with storage, reuse and/or rejuvination of Protein A.
    Type: Grant
    Filed: July 5, 2006
    Date of Patent: August 23, 2011
    Assignee: Decon Labs, Inc.
    Inventor: Tim Gordon
  • Patent number: 8003402
    Abstract: The invention provides an accurate, economical, automatable, high throughput method for the determination of the concentration of glycosaminoglycan anticoagulants, including low molecular weight heparin (LMWH) anticoagulants, in aqueous solutions. A method for cleaning a unit of manufacturing equipment used in the preparation of a LMWH to obtain an acceptable residual concentration of LMWH is further provided.
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: August 23, 2011
    Assignee: Active BioMaterials, LLC
    Inventors: Ralph Yamamoto, Yumi Kim