Preservative, Buffer, Anticoagulant Or Diluent Patents (Class 436/18)
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Patent number: 6821779Abstract: The instant methods pertain to improved methods for storing neural cells, preferably dissociated neural cells, prior to their use in transplantation and to the cells obtained using such methods. One embodiment pertains to methods for storing the neural cells in medium lacking added buffer or added protein, other embodiments feature neural cells which are maintained at 4° C. prior to cryopreservation and have comparable viability and/or functionality to freshly harvest cells. In addition, methods for storing and/or transplantation of porcine neural cells are described.Type: GrantFiled: August 17, 2001Date of Patent: November 23, 2004Assignees: University Hospital Groningen, Inc., Diacrin, Inc.Inventors: Jan Koopmans, Douglas B. Jacoby, Jonathan Dinsmore
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Patent number: 6812032Abstract: An apparatus is provided for hematology testing, which has a sensing unit defining a counting orifice for the flow of a blood sample through the counting orifice to analyze the blood sample, and a pump unit having three syringes. A first syringe is coupled in fluid communication with the sensing unit on the inlet side of the counting orifice for injecting a stream of blood sample through the counting orifice. A second syringe is coupled in fluid communication with the sensing chamber on the inlet side of the counting orifice for simultaneously injecting a sheath of fluid surrounding the sample stream on the inlet side of the counting orifice. And a third syringe is coupled to the sensing chamber on the outlet side of the counting orifice for aspirating a sheath of fluid from the sensing chamber surrounding the sample stream on the outlet side of the counting orifice.Type: GrantFiled: March 16, 1998Date of Patent: November 2, 2004Assignee: CDC Technologies, Inc.Inventors: Edward Lawrence Carver, Jr., David Charles DeCava
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Patent number: 6794152Abstract: An improved reagent system for preparation of cells for flow cytometry having a physiologically compatible salt solution composition including a lysing agent, an agent for minimizing white blood cell lysis, and optionally a preservative.Type: GrantFiled: December 22, 2000Date of Patent: September 21, 2004Assignee: Streck Laboratories Inc.Inventors: Wayne L. Ryan, Paula B. Turpen
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Publication number: 20040180444Abstract: An aqueous control solution is disclosed for use with a spectrophotometer or photometric test strip that includes a predetermined amount of an analyte, a hydrophobic reference dye and a surfactant. In one embodiment, the aqueous control solution is applied to a photometric test strip having a chemical that oxidizes glucose and consequently forms hydrogen peroxide which reacts with an indicator dye that is also present on the strip. In this embodiment, the control solution includes a predetermined amount of glucose, an infrared reference dye, sodium dodecyl sulfate, and the indicator dye, such as sulforhodamine B.Type: ApplicationFiled: March 11, 2003Publication date: September 16, 2004Applicant: Bionostics, Inc.Inventors: Minna A. Rannikko, Donna M. Murray, Donna M. Rafferty
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Patent number: 6790603Abstract: Blood platelets stored in an isotonic, balanced salt solution under standard storage conditions retain function after ten days of storage when micormolar or nanomolar amounts of pentose are added to the solution. The preferred pentoses are D-Ribose, xylulose-5-phosphate and the pentose-related alcohol xylitol.Type: GrantFiled: December 21, 2000Date of Patent: September 14, 2004Assignee: Bioenergy, Inc.Inventors: Daniel G. Ericson, John St. Cyr
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Method and system for creating a mercury halide standard for use in testing a mecury analyzer system
Publication number: 20040152198Abstract: A method and system are provided for creating a mercury halide standard, and for using that standard to test a mercury analyzer system. Gaseous elemental mercury of a known concentration from a gas cylinder and a halogen donor are fed to a reaction chamber where they react to form mercury halide. The mercury halide is fed to a mercury analyzer system where it is converted to reform gaseous elemental mercury which is then measured by a mercury analyzer. By comparing either the amount of elemental mercury supplied to the reaction chamber or the amount of mercury halide formed in the reaction chamber with the amount of elemental mercury converted from the mercury halide, the ability of the mercury analyzer system to convert mercury halide to gaseous elemental mercury can be evaluated.Type: ApplicationFiled: February 3, 2003Publication date: August 5, 2004Applicant: Spectra Gases, Inc.Inventors: Stephen B. Mandel, Scott M. Shields -
Patent number: 6764482Abstract: A dual-filled twin bag, a packaging including the twin bag, and a method for forming the packaged twin bags are provided. Each of two bags, a solution bag (10) and a drain bag (12) for a system used for a peritoneal dialysis procedure, are filled, at least partially, with a solution (14). A tubing set (16) connects the two filled bags (10,12) and may also be filled with solution (14) which is added to the bags (10,12) and the tubing set (16) during the manufacturing process. Alternatively, the tubing set (16) may be filled with air. The tubing set (16) is sandwiched between the solution bag (10) and the drain bag (12) and subsequently packaged in an overpouch (24). The method for forming the package including the bags filled with solution is simplified and the overall package size is reduced due to the sandwiched tubing set (16) between the solution bags (10,12) and the provision of solution in both bags (10,12).Type: GrantFiled: October 29, 2002Date of Patent: July 20, 2004Assignee: Baxter International Inc.Inventors: Michael R. Keilman, Walter A. Makaryk, Joseph A. Giertych
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Publication number: 20040132207Abstract: The object of the present invention is to provide convenient methods that enable proper analyses of blood components by stabilizing those components, and a container that is useful for the methods.Type: ApplicationFiled: January 6, 2003Publication date: July 8, 2004Inventors: Kazuhisa Arima, Masaharu Kojima, Shinya Nakano, Tomoko Gunji
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Publication number: 20040133146Abstract: A device for treating a fluid-like sample, in particular whole blood, with treatment fluid. The device comprises a tubular container with a first piston and a second piston, with a piston gap between them in which the treatment fluid is located in the initial position. The device further comprises an inlet port to which a capillary can be connected and an outlet port to which a filter can be connected.Type: ApplicationFiled: October 31, 2003Publication date: July 8, 2004Inventors: Arjan Jacob Broek, Max van den Berg, Felix Wilhelmus Marie de Rooij
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Publication number: 20040121469Abstract: The present invention provides storage stable, aseptic buffer solutions suitable for gamma sterilization. The buffer solutions comprise benzethonium chloride as a biocide, and avoid the acidification effects and the formation of precipitates that can be caused by gamma sterilization.Type: ApplicationFiled: November 25, 2003Publication date: June 24, 2004Inventors: Matthew Romey, Mark Takagi
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Patent number: 6750060Abstract: A sheath liquid for use with a particle analyzer has a refractive index of 1.3340 or more at the wavelength of sodium D line at 25° C.Type: GrantFiled: March 25, 2003Date of Patent: June 15, 2004Assignee: Sysmex CorporationInventors: Masatsugu Ozasa, Junya Inoue, Masakazu Fukuda
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Patent number: 6740527Abstract: Cyanide-free reagents for the determination of hemoglobin and leukocytes present in a blood sample comprise an aqueous solutions of at least one quaternary ammonium salt, preferably selected from the group of alkyltrimethylammonium salts, alkyldimethylbenzylammonium salts or alkylpyridium salts consisting of tetradecyltrimethyl ammonium bromide (TTAB), dodecyltrimethyl ammonium chloride, cetyltrimethyl ammonium bromide, hexadecyltrimethyl ammonium bromide and benzalkonium chlorides, and hydroxylamine salts, especially hydrochloride, sulfate and phosphates and other acid salts. The method involves mixing the reagent with a diluent-diluted blood sample, presenting it to an absorbance spectrophotometer and measuring the resulting optical density as an indicator of hemoglobin concentration. This cyanide-free reagent could be used solely for hemoglobin determinations, or, it can also be used in leukocyte counting and sizing using hematology instrumentation.Type: GrantFiled: May 27, 1997Date of Patent: May 25, 2004Assignee: Abbott LaboratoriesInventors: Show-Chu Wong, Sylvia Khoo
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Patent number: 6723497Abstract: A dehydrated composition is provided that includes freeze-dried platelets. The platelets are loaded with trehalose which preserves biological properties during freeze-drying and rehydration. The trehalose loading is conducted at a temperature of from greater than about 25° C. to less than about 40° C., most preferably at 37° C., with the loading solution having trehalose in an amount from about 10 mM to about 50 mM. These freeze-dried platelets are substantially shelf-stable and are rehydratable so as to have a normal response to an agonist, for example, thrombin, with virtually all of the platelets participating in clot formation within about three minutes at 37° C.Type: GrantFiled: April 5, 2001Date of Patent: April 20, 2004Assignee: The Regents of the University of CaliforniaInventors: Willem F. Wolkers, John H. Crowe, Fern Tablin, Ann E. Oliver, Naomi J. Walker, Nelly Tsvetkova
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Patent number: 6716392Abstract: The present invention relates to the preservation of a liquid biological sample. The biological sample is exposed to a preservative containing at least about 0.15 g of sodium benzoate and at least about 0.025 g of citric acid per 100 ml of sample. The biological sample may be collected in a vessel or an absorbent mass. The biological sample may also be exposed to a substrate and/or a vehicle.Type: GrantFiled: July 28, 2000Date of Patent: April 6, 2004Assignee: The United States of America as represented by the United States National Aeronautics and Space AdministrationInventors: Lakshmi Putcha, Ramalingeshwara R. Nimmagudda
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Patent number: 6716632Abstract: A transport/preservation system has been found which provides safe, effective transport for patient specimens and bodily fluids, and functions as a preservative for biological reagents, therapeutics and personal care products. The transport/preservative formulations can also be used as safe, effective sanitizers on surfaces, equipment, and appliances. The transport preservation formulations comprising a biguanide and one or more other antimicrobial agents are cidal to microorganisms when present in a sample or on a surface to be sanitized.Type: GrantFiled: May 7, 2002Date of Patent: April 6, 2004Inventor: Gordon L. Dorn
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Patent number: 6713245Abstract: The instant methods pertain to an improved methods for storing neural cells, preferably dissociated neural cells, prior to their use in transplantation and to the cells obtained using such methods. One embodiment pertains to methods for storing the neural cells in medium lacking added buffer or added protein, other embodiments feature neural cells which are maintained at 4° C. prior to cryopreservation and have comparable viability and/or functionality to freshly harvested cells. In addition, methods for storing and/or transplantation of porcine neural cells are described.Type: GrantFiled: July 6, 1998Date of Patent: March 30, 2004Assignees: Diacrin, Inc., University Hospital GroningenInventors: Jan Koopmans, Douglas B. Jacoby, Jonathan Dinsmore
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Patent number: 6709868Abstract: A method for quantitatively measuring white blood cell count involves capture of white blood cells from a fluid sample by a retainer, removal of the red blood cells and other interfering substances by a wash solution, and reading the result of a color reaction in which an ester which is present on the white blood cells cleaves a chromogenic substrate which produces a water insoluble dye. The apparatus for use in the present method includes a retainer for white blood cells that has a dye substrate immobilized therein and an absorption layer that wicks and takes up all excess washing solution flowing past the sample.Type: GrantFiled: May 20, 2002Date of Patent: March 23, 2004Assignee: Portascience Inc.Inventors: Wai Tak Law, Yuri Nikolyukin, Inna Nikolyukin
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Patent number: 6706526Abstract: A low formaldehyde containing aqueous blood diluent contains an effective amount of ethylenediamine tetraacetic acid, ethylenediamine tetraacetic acid derivative, or combinations thereof; an effective amount of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one; and an effective amount of 5-bromo-5-nitro-1,3-dioxane. Advantageously, less than about 1 parts per million of formaldehyde is produced in this multipurpose diluent. A method of analyzing a blood sample containing blood cells is conducted by forming a diluted blood sample by mixing a blood sample containing blood cells with this diluent and analyzing the diluted blood sample to determine a physical parameter of the blood cells.Type: GrantFiled: January 24, 2002Date of Patent: March 16, 2004Assignee: Coulter International Corp.Inventors: Russell F. Lang, Iris L. Payan, Barbara G. Murza, Luisa C. Oramas
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Publication number: 20040048384Abstract: A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling.Type: ApplicationFiled: July 2, 2003Publication date: March 11, 2004Inventors: Frank A. Augello, Lynne Rainen, Matthew Walenciak, Uwe Oelmuller, Ralf Wyrich, Helge Bastian
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Patent number: 6699718Abstract: The invention is a method, reagent and test cartridge for the determination of the clotting time of a blood sample by means of a reagent containing tissue factor and a sulfatide. In an alternative embodiment, the reagent may contain tissue factor and at least one of the group consisting of a phosphatide and a sulfatide. This invention is preferably used to monitor the effectiveness of heparin therapy in patients that have been administered low to moderate heparin doses to achieve blood heparin levels from 0 to about 3 U/mL, and may also be used for determining clotting time at higher heparin levels of up to about 6 U/mL.Type: GrantFiled: August 24, 2000Date of Patent: March 2, 2004Assignee: Roche Diagnostics CorporationInventor: Berndt B. Bruegger
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Publication number: 20040038424Abstract: A stabilization reagent composition, particularly desirable for stabilizing blood samples containing platelets, comprises reactants that generate multiple species of formaldehyde-ammonium complexes; at least one inhibitor of phosphatase enzymatic activity; and at least one inhibitor of protease enzymatic activity. Blood samples and other tissues are stabilized by this composition. Such stabilized samples are produced by methods for stabilizing the tissue by contacting the sample with the composition. Methods for assessing the efficacy of such stabilizing reagents are also included in this invention.Type: ApplicationFiled: August 23, 2002Publication date: February 26, 2004Inventor: John A. Maples
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Patent number: 6682931Abstract: This invention relates to a method for improving the growth and regeneration potential of embryogenic cell and tissue cultures of coniferous plants retrieved from cryopreservation. In particular, this invention relates to the use of abscisic acid in the post-cryopreservation recovery medium to improve both the growth and somatic embryo production of embryogenic cell and tissue cultures of conifers, thereby enabling more rapid proliferation of the embryogenic cultures and a subsequent increase in the yield of somatic embryos. This method is well-suited for employment with a number of biotechnological uses of embryogenic cultures of coniferous plants retrieved from cryopreservation, including use with embryogenic cultures of coniferous plants and with genetically transformed embryogenic cultures of coniferous plants for producing clonal planting stock useful for reforestation.Type: GrantFiled: May 19, 2000Date of Patent: January 27, 2004Assignee: MeadWestvaco CorporationInventors: Michael Ryan Becwar, Sharon Anne Krueger
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Publication number: 20040014222Abstract: The present invention provides reagents for use in an automated environment for cell conditioning of biological samples wherein the cells or tissues are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents comprise components optimized to facilitate molecular access to cells and cell constituents within the biological sample. The present invention also provides reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents comprise components optimized to facilitate removal or etching of the embedding media from the biological sample.Type: ApplicationFiled: March 7, 2001Publication date: January 22, 2004Inventors: Penny Towne, Joseph Utermohlen
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Patent number: 6673607Abstract: A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering the stored biological material from storage. Carbohydrate sugars such as trehalose, sucrose, fructose, dextran, and raffinose, may be used as bio-protective agents.Type: GrantFiled: May 16, 2001Date of Patent: January 6, 2004Inventors: Mehmet Toner, Ali Eroglu, Thomas Toth
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Publication number: 20030207458Abstract: A reagent for detecting exogenous oxidants in urine comprises tetramethylbenzidine, an acidic buffer, a catalyst, a medium for carrying out chemical reactions, and a resinous material. The reagent may be utilized in liquid form or may be applied to a test strip.Type: ApplicationFiled: May 6, 2002Publication date: November 6, 2003Inventor: Taya Sookbumroong
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Patent number: 6641992Abstract: An aqueous solution for preserving tissues and organs and methods of use for the same are disclosed.Type: GrantFiled: November 19, 2001Date of Patent: November 4, 2003Assignee: Cair L.G.L.Inventors: Georges Antoine Lopez, Silvina Ramella Virieux
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Patent number: 6635485Abstract: The invention concerns calibrators or calibration solutions which are based on a human serum matrix and which are used in a method for detecting cytokeratin, a process for producing them, a method for stabilizing cytokeratin and an immunological method for detecting cytokeratin in a sample.Type: GrantFiled: October 27, 2001Date of Patent: October 21, 2003Assignee: Roche Diagnostics GmbHInventors: Apostolos Kyriatsoulis, Gunter Pappert, Ellen Moessner, Norbert Franken, Michael Rottmann, Heinz Bodenmueller
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Publication number: 20030194810Abstract: An NMR buffer is particularly useful for biomolecules that require a certain amount of salt in the buffer solution. A primary buffer component and a titrating component are selected based on low ion mobility. These selections allow for a lower conductivity buffer without reducing the salt content. As such, a higher sensitivity experiment results.Type: ApplicationFiled: April 12, 2002Publication date: October 16, 2003Applicants: Bruker Biospin Corporation, The Regents of the Universty of CaliforniaInventors: Volker Dotsch, Richard S. Withers
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Patent number: 6632677Abstract: An air-barrier agent for an aqueous reagent or an aqueous specimen having as an effective component a mixture of a chain hydrocarbon and a silicone oil immiscible with the aqueous reagent as well methods of using and making the same.Type: GrantFiled: March 9, 2001Date of Patent: October 14, 2003Assignee: Daiichi Pure Chemicals Co., Ltd.Inventors: Masahiro Sekiguchi, Toshikatsu Abe, Koji Ushizawa
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Patent number: 6632676Abstract: A novel reagent system for use with automated and semi-automated hematology analyzers including an essentially isotonic blood diluting reagent, a blood cell lysing and hemoglobin conversion reagent, and a second lysing reagent for differentiating white blood cells into classes by size and functional characteristics. The diluent reagent enhances properties for counting and sizing blood specimens, while stabilizing cellular volume and cellular integrity for many hours. The blood cell lysing reagent removes red blood cells and enables subsequent enumeration of white blood cells and simultaneous determination of hemoglobin without use of the toxic cyanide anion. The third lysing reagent and a companion quenching differentiates blood cells into classes by size and functional characteristics, based on d.c. impedance volume, conductivity/opacity and light scatter measurements. The companion quenching reagent adjusts pH and conductivity of the final measurement solution to match the analyzer system requirements.Type: GrantFiled: September 24, 1999Date of Patent: October 14, 2003Assignee: Clinical Diagnostic SolutionsInventors: Harold Richardson Crews, James Harrison Carter, II, Ted Sena
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Patent number: 6623921Abstract: A method for measurement of the hepatitis C virus (HCV) characterized by measuring HCV core antigen and HCV core antibody by their binding with probes in the presence of an anionic surfactant or a non-ionic surfactant, or both.Type: GrantFiled: April 26, 2002Date of Patent: September 23, 2003Assignee: Advanced Life Science Institute, Inc.Inventors: Katsumi Aoyagi, Chiharu Ohue, Kumiko Iida, Shintaro Yagi
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Patent number: 6623982Abstract: Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for significantly enhancing the magnetic isolation of low antigen density target cells from biological samples are disclosed.Type: GrantFiled: July 12, 1999Date of Patent: September 23, 2003Assignee: Immunivest CorporationInventors: Paul A. Liberti, Galla Candra Rao, Leon W. M. M. Terstappen
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Patent number: 6620627Abstract: Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for significantly enhancing the magnetic isolation of low antigen density target cells from biological samples are disclosed.Type: GrantFiled: October 31, 2000Date of Patent: September 16, 2003Assignee: Immunivest CorporationInventors: Paul A. Liberti, Galla Candra Rao, Leon W. M. M. Terstappen
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Publication number: 20030170907Abstract: The invention relates to an enhancement solution for an assay technology using lanthanide ions or their chelates as labels and dissociative fluorescence enhancement as a tool for detection, wherein said enhancement solution comprises a &bgr;-dietone of formula I 1Type: ApplicationFiled: March 8, 2002Publication date: September 11, 2003Inventors: Ilkka Hemmila, Kaj Blomberg, Veli-Matti Mukkala, Harri Hakala
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Publication number: 20030170618Abstract: The present invention is directed to an antigen diluent or buffer for antigens, in particular HCV recombinant antigens, comprising a reducing agent. The antigen diluent or buffer serves as a stabilizing buffer for the antigens. The present invention is also directed to antigen diluents or buffers for use in an automated immunoassay.Type: ApplicationFiled: January 28, 2003Publication date: September 11, 2003Inventors: David Y. Chien, Phillip Arcangel, Stephen Tirell, Wanda Zeigler
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Patent number: 6617170Abstract: A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling.Type: GrantFiled: October 30, 2001Date of Patent: September 9, 2003Assignees: Becton, Dickinson and Company, Qiagen GmbHInventors: Frank A. Augello, Lynne Rainen, Matthew Walenciak, Uwe Oelmüller, Ralf Wyrich, Helge Bastian
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Publication number: 20030166290Abstract: A low formaldehyde containing aqueous blood diluent contains an effective amount of ethylenediamine tetraacetic acid, ethylenediamine tetraacetic acid derivative, or combinations thereof; an effective amount of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one; and an effective amount of 5-bromo-5-nitro-1,3-dioxane. Advantageously, less than about 1 parts per million of formaldehyde is produced in this multipurpose diluent. A method of analyzing a blood sample containing blood cells is conducted by forming a diluted blood sample by mixing a blood sample containing blood cells with this diluent and analyzing the diluted blood sample to determine a physical parameter of the blood cells.Type: ApplicationFiled: January 24, 2002Publication date: September 4, 2003Inventors: Russell F. Lang, Iris L. Payan, Barbara G. Murza, Luisa C. Oramas
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Patent number: 6602718Abstract: A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes an effective amount of at least one stabilizing agent. The stabilizing agent is able to stabilize nucleic acids in the biological sample at the point of collection to prevent enzymatic degradation of the nucleic acids. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, and mixtures thereof.Type: GrantFiled: November 8, 2000Date of Patent: August 5, 2003Assignees: Becton, Dickinson and Company, Qiagen GmbHInventors: Frank A. Augello, Lynne Rainen, Matthew Walenciak, Uwe Oelmüller, Ralf Wyrich, Helge Bastian
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Publication number: 20030124733Abstract: A method for preserving a sample is described. A method for preserving a sample device such as microarrays, slides and membranes is described. The preservation is achieved by applying a coating composition to a sample or sample device, and curing the coating composition. Candidate coating materials for forming the coating compositions are described. Preferably, the coating composition is an optically clear, solidifying solution. Also described are preservation kits which provide materials and instructions for the preservation of sample devices. Calibration devices are also described.Type: ApplicationFiled: September 5, 2002Publication date: July 3, 2003Applicant: Genicon Sciences CorporationInventors: Paul Bushway, Laurence Warden, Todd Peterson, David E. Kohne, Juan Yguerabide
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Patent number: 6582574Abstract: pK-matched buffers, each containing two effective buffering components: one weak base and one weak acid which have similar pKa at 25° C. (within 0.3 pK units On agarose gels, the buffers in various concentrations were tested for separation of double-stranded DNA fragments with various DNA markers, agarose gel concentrations, and field strengths. Mobility was inversely proportional to the logarithm of molecular weight. The buffers provided high resolution without smearing at more dilute concentration than is possible with standard TAE (Tris/Acetate, pH 8.0) or TBE (Tris/Borate, pH 8.3) buffers. The buffers were also tested in 7M urea denaturing LongRanger™ sequencing gels and in non-denaturing polyacrylamide SSCP gels. The pK-matched buffers provide good separation and high resolution, at a broad range of potential pH values.Type: GrantFiled: March 28, 2000Date of Patent: June 24, 2003Assignee: City of HopeInventors: Qiang Liu, Steve S. Sommer
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Patent number: 6579723Abstract: A method for purifying factor VIII/vWF complex or free vWF by immunoaffinity chromatography in a form suitable for use as a medicament. Factor VIII/vWF complex or free vWF is recovered from an immunoaffinity adsorbent by using an eluting agent containing a zwitterionic species. The presence of the zwitterionic species allows for the use of mild conditions throughout the preparation, facilitating retention of molecular integrity, activity, and incorporation of the recovered proteins into pharmaceutical preparations without the need for additional stabilizers or preservatives.Type: GrantFiled: October 21, 1999Date of Patent: June 17, 2003Assignee: Baxter AktiengesellschaftInventors: Artur Mitterer, Christian Fiedler, Bernhard Fischer, Friedrich Dorner, Johann Eibl
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Patent number: 6579688Abstract: Compositions for stabilizing polypeptides or antigens are described. These compositions are useful for stabilizing polypeptides or antigens stored in aqueous formulations. Such formulations can be used for various analytical or other methods.Type: GrantFiled: December 14, 2000Date of Patent: June 17, 2003Assignee: Biostar, Inc.Inventors: Jeffrey W. Steaffens, Laura Panzarella
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Patent number: 6573102Abstract: A lytic reagent composition for measuring nucleated blood cells in a blood sample is described. The lytic reagent composition contains a quaternary ammonium surfactant, an ethoxylated phenol, and an ethoxylated alcohol. When mixed with a blood sample, the lytic reagent composition lyses red blood cells and enables a differentiation of nucleated red blood cells from other cell types by DC impedance measurement. The lytic reagent composition can further contain an organic ligand for determining total hemoglobin concentration of a blood sample photometrically. Further disclosed is a lytic reagent system including the lytic reagent composition and a diluent. In addition, a single reagent composition containing salts is also disclosed, which can be used without a separate diluent. The lytic reagent compositions can be used for concurrent measurement of nucleated red blood cells, WBC, and hemoglobin of a blood sample.Type: GrantFiled: July 27, 2001Date of Patent: June 3, 2003Assignee: Coulter International Corp.Inventors: Yi Li, Jing Li
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Patent number: 6569682Abstract: An improved blood suspension media for hematological compositions, having particular utility with a red blood cell component for devices using electronic and optical means for blood determinations, and processes for using the suspension media. The suspension media finds particular utility in providing the hematology control product with a stable and consistent MCV and RDW for an extended product shelf life.Type: GrantFiled: July 27, 2001Date of Patent: May 27, 2003Assignee: Coulter International Corp.Inventors: Michael Elliott, Dana B. Jacobs, Nery Ortiz, Wayne Goldson, Penny Whitley, Theodore J. Gerula
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Patent number: 6569615Abstract: The present invention provides for compositions and methods for the preservation of tissues and organs ex vivo and in situ. In addition, the present invention provides for kits that may be used in the preparation of the solutions of the present invention.Type: GrantFiled: April 10, 2000Date of Patent: May 27, 2003Assignee: The United States of America as represented by the Department of Veteran's AffairsInventors: Hemant Thatte, Kunda Biswas, Shukri Khuri, Thomas Michel
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Patent number: 6562630Abstract: A method is provided for preparing preservative-free allergen test solutions in a prepared sterile environment. A sterile environment is prepared by utilizing a disinfectant wipe and a sterile barrier field. An antigen is added to a diluent to form a solution of the antigen by dispersing or suspending the antigen in the diluent. The solution is subjected to triple filtration under specific and sequential conditions to provide consistent and preservative-free allergen test solutions. Shelf life is extended by storing the solution at a temperature below 5° F. or by lyophilizing the allergen test solution.Type: GrantFiled: October 12, 2001Date of Patent: May 13, 2003Inventors: Thomas H. Bryant, Nabil K. Aboukhair
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Publication number: 20030064518Abstract: Protective solution for use in a fixation method for the paraffin section technique and comprising (i) at least one amino acid and (ii) at least one sugar compound.Type: ApplicationFiled: September 28, 2001Publication date: April 3, 2003Inventor: Jurgen Olert
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Patent number: 6537745Abstract: The present invention is directed to an antigen diluent or buffer for antigens, in particular HCV recombinant antigens, comprising a reducing agent. The antigen diluent or buffer serves as a stabilizing buffer for the antigens. The present invention is also directed to antigen diluents or buffers for use in an automated immunoassay.Type: GrantFiled: February 2, 2001Date of Patent: March 25, 2003Assignee: Chiron CorporationInventors: David Y. Chien, Phillip Arcangel, Stephen Tirell, Wanda Zeigler
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Patent number: 6534016Abstract: The present invention is an additive preparation which contains an additive, an organic acid, a metal carbonate compound, and a surfactant agent which is capable of rendering the surface of a collection device to have properties that cause the surface to repel the adsorption of components of a body fluid sample. The preparation effervesces when in contact with a body fluid sample, thereby efficiently dispersing the additive and surfactant in a body fluid sample.Type: GrantFiled: November 21, 2000Date of Patent: March 18, 2003Inventors: Richmond Cohen, Ajit N. Dastane
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Patent number: RE38131Abstract: A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers: (a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, (b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and (c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, and a buffer for maintaining pH at 4 to 6.Type: GrantFiled: October 15, 2001Date of Patent: June 3, 2003Assignee: Sysmex CorporationInventors: Kinya Uchihashi, Yoshiro Ikeuchi, Atsushi Shirakami, Yukio Hamaguchi