Abstract: An automated analyzer according to an embodiment includes a probe. The probe has a step part configured to be provided between a lower shaft and an upper shaft, and to be formed such that the outer diameter changes. A descending controller lowers the probe from a predefined position that positions the step part above the liquid surface to an operating position that positions the step part below the liquid surface. A ascending controller raises the probe at a high speed from the operating position until immediately before the step part reaches the position of the liquid surface, subsequently raises the probe at a low speed until the step part passes through the liquid surface, and raises the probe at a higher speed than the low speed from immediately after the step part passes through the position of the liquid surface up to the predefined position.

Abstract: An interface apparatus between a pneumatic mail system and a feeding system of biological products to a laboratory automation system is described, the apparatus comprising a capsule suitable for being conveyed in a pneumatic mail system which accommodates therein one or more biological material containers, said capsule opening and connecting to a device for transferring said biological material containers contained in said capsule into at least one recruiting device of said biological product containers, said at least one recruiting device being used to load said containers of biological products into a positioning device interfaced with a gripping device of said biological product container for transferring said biological product container on an automatic conveyor belonging to a laboratory automation system. Said capsule has an inner chamber containing foam elements adapted to withhold said biological product containers present in said capsule.

Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.

Abstract: A liquid aspirating and dispensing apparatus and method of using the apparatus. Among other elements, the apparatus includes a liquid handling device having one or more channel members, each of which has a removable liquid holding tip attached, and a signal transmitting device. A signal transmitted from the signal transmitting device may be used in a variety of ways to yield information regarding the performance of the apparatus. This information may be provided via direct detection of the signal by an operator of the apparatus. Alternatively, the signal may be detected by a programmable signal detecting device, interpreted by the apparatus, and then displayed in a form that is understandable to the operator. Furthermore, the liquid holding tip may be modified to enable different forms of signal transmission.

Abstract: Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing.

Abstract: A method for fixation of organic tissue samples includes immersing a tissue sample in a solution of Formalin at a temperature of between 2° C. and 10° C. for a first time period (A), and immersing the tissue sample in a cold fixation dehydrating agent for a second time period (B) following the first time period. An automated tissue fixation system for fixating organic tissue samples in Formalin is also described.

Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.

Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

Abstract: A microfluidic device and a control method, the microfluidic device including a platform including a chamber configured to accommodate a sample, a channel connected to the chamber, and a metering unit connected to the chamber by the channel and configured to meter an amount of the sample, wherein the metering unit includes a metering chamber configured to measure the amount of the sample, and an accommodation chamber connected to the metering chamber and configured to accommodate the sample to prevent the sample from overflowing out of the metering chamber.

Abstract: A reagent container pack for storing, preserving, and automatically unsealing and resealing a plurality of reagent containers in a reagent container pack on-board an automated clinical sample analyzer for analyzing analytes in a body fluid.

Abstract: A diagnostic analyzer system includes a linear track, at least one pipetting device, and at least one diagnostic module. The linear track includes a pre-treatment lane disposed parallel to at least one processing lane. The linear track moves reaction vessels, containing samples, held by the pre-treatment lane and by the at least one processing lane. The pre-treatment lane pre-treats the samples in the reaction vessels of the pre-treatment lane. The pre-treatment lane is not connected to any diagnostic module for testing the samples in the reaction vessels of the pre-treatment lane. The at least one pipetting device transfers the pre-treated samples from the reaction vessels in the pre-treatment lane to the reaction vessels in the at least one processing lane. The at least one diagnostic module is connected to the at least one processing lane for testing the pre-treated samples transferred into the reaction vessels in the at least one processing lane.

Abstract: A device for collecting, treating and dispensing a biological sample is described. Use of the device permits integration of sample collection, sample treatment, and sample dispensing.

Abstract: The invention relates to a sampling valve, for taking samples of fluid from a common collecting vessel in order to perform a plurality of analyses using reagents. The valve of the invention comprises two members that are movable relative to each other, comprising a sampling member having sampling loops, each intended to receiving an aliquot of fluid, and a connection member capable of assuming at least three functional positions relative to the sampling member. These three positions enable the delayed access to two separate sampling circuits in order to fill them and to dispense reagents to the loops that make them up.

Abstract: An apparatus for preparation of samples for analysis comprising includes structure (21) for interchangeably locating first racks of sample receptacles that contain samples to be prepared for analysis and second racks include racks of different configuration racks for of sample receptacles which second that are respectively transferable to different analysis instruments, and for receiving and positioning a plurality of reagent vessels at known respective positions. An assembly of devices (50,60) is actuable to transfer samples from sample receptacles of the first rack to sample receptacles of the second rack, while carrying out a sample preparation operation selected from a plurality of pre-programmed sample preparation operations, wherein the apparatus is configured to identify which of said the second racks of different configuration is located at said the structure, and to adapt said the transfer and said the selected sample preparation operation to suit the identified rack.

Abstract: A sample rack transport system comprising: a plurality of transport apparatuses which are connected so as to transport a sample rack to a plurality of sample processing apparatuses; and a control apparatus which communicates with the plurality of transport apparatuses and controls the transport of the sample rack by the plurality of transport apparatuses, wherein at least one of the plurality of transport apparatuses includes a transmission switch which is operated by a user to transmit a signal to the control apparatus, and when the transport of the sample rack has stopped due to a trouble which occurred in one of the plurality of transport apparatuses, responsive to an operation of a transmission switch of another transport apparatus, the control apparatus restarts the transport of the sample rack by the plurality of transport apparatuses. Also, a method for transporting a sample rack.

Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.

Abstract: We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

Abstract: An absorbing zone for establishing and/or maintaining fluid transport through or along at least one fluid passage is manufactured on the basis of a non-porous substrate having a surface, the non-porous substrate having projections substantially perpendicular to the surface, and the projections having a height, diameter and a distance or distances between the projections such, that lateral capillary flow of the fluid in the zone is achieved.

Abstract: The present invention concerns a pipette comprising a mechanism with which the stroke length of a piston can be changed by rotating the piston rod. The pipette comprises a locking mechanism which prevents the rotation of the piston rod when the piston has been pushed a certain distance downwards from its upper position, but does not prevent the rotation when the piston is in its upper position.

Abstract: An automated liquid collection workstation is provided. The workstation includes a processor, a peristaltic pump, a valve actuator, and an algorithm. The peristaltic pump and the valve actuator are in electrical communication with the processor. The valve actuator can move a plurality of valves, when disposed therein, among an off position, a flush position, and a collection position. The algorithm is resident on the processor and is configured to: move all of the valves to the off position and place the pump in an off state when no sampling or flushing is required, move all of the valves to the flush position and place the pump in an on state for a predetermined flush time period when flushing is required, and move a respective one of the valves to the collection position, move any of the valves upstream of the respective valve to the flush position, and place the pump to the on state for a predetermined collection time period when collection is required.

Abstract: A holding unit pivotally holds a plurality of reagent bottles which store reagents. A reagent case houses the holding unit and has an edge higher than at least the housed holding unit. The reagent cover is a cover for closing the reagent case. A cooling unit is mounted on one outer surface of the reagent case and cools air in the reagent case closed by the reagent cover through one outer surface. A circulating unit is mounted on the holding portion and circulates the cooled air in the reagent case closed by the reagent cover.

Abstract: A delivery apparatus for selectively delivering one or more liquid reagents into a reaction or test chamber (2), especially of an assay apparatus, the apparatus comprising: one or more respective storage chambers (5,6) for containing the one or more liquid reagents and arranged generally above the reaction or test chamber (2); and a plunger element (4) arranged and operable for insertion into the mouth of a selected storage chamber so as to displace a selected reagent from therewithin into the reaction or test chamber (2) generally therebelow by gravitational liquid overflow from the mouth of the chamber. The apparatus may conveniently be provided as a discrete delivery unit, with the storage chambers (5,6) pretilled with the selected reagents.

Abstract: The present invention concerns a tape magazine especially for a hand-held device for analyzing a body fluid with a test tape that can be unwound from a storage unit and wound onto a waste unit where the waste unit can be driven in order to wind forward the test tape. The invention provides that a lock which keeps the test tape under tension is integrated in the tape magazine at least on the storage unit. The present invention also concerns a hand-held device with such a tape magazine. The present invention also concerns a hand-held device with a mechanical drive for the test tape and a disposable hand-held device with polymer-based electronic components.

Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

Abstract: A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid to introduce the first liquid into a second vessel by using a tube.

Abstract: Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing, elution, assay assembly, assay detection, and cleaning after reactions and between samples.

Abstract: In a microfluidic assembly, a microfluidic device is provided with a body in which at least a first inlet for loading a fluid for analysis, and a buried area in fluid communication with the first inlet are defined. An analysis chamber is in fluid communication with the buried area and an interface cover is coupled in a fluid-tight manner above the microfluidic device. The interface cover is provided with a sealing portion in correspondence to the analysis chamber, operable to assume a first configuration, in which it leaves the analysis chamber open, and a second configuration, in which it closes the analysis chamber in a fluid-tight manner.

Abstract: A lab member for use in a laboratory liquid handling system including a pipetting module and a drive system, the pipetting module including first and second pipettors, the first and second pipettors including first and second pipettor shafts, respectively, a first pipetting tip extending from an end of the first pipettor shaft, and a second pipetting tip extending from an end of the second pipettor shaft, includes a lab object and first and second integral adapter structures. The first and second adapter structures are configured to engage the first and second pipettor shafts, respectively. The first adapter structure is configured to releasably secure the lab member to the first pipettor shaft.

Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnetic field wherein droplet operations do not allow magnetically responsive beads to be introduced into a region of the magnetic field which is sufficiently non-uniform to cause bead clumping resulting in a more homogenous distribution of the beads in the droplet. Other embodiments are also provided.

Abstract: In a dispensing apparatus for dispensing a liquid, such as a sample solution, on a substrate, such as a glass slide, the dispensing apparatus includes a capillary provided with a distal end and a proximal end, a pump unit configured to pump an operating liquid into the capillary and to pump the operating liquid out of the capillary, and a controller configured to control the pump unit so as to change a position of a liquid surface of the operating liquid in the capillary so that a predetermined volume of liquid is suctioned from the distal end into the capillary and the liquid suctioned in the capillary is discharged from the distal end. As a result, it is possible to precisely dispense extremely small volume amounts of liquids such as a nanoliter.

Abstract: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

Abstract: This invention provides an analyzer that has a liquid dispense pipette dispensing liquids with higher accuracy and precision at higher speeds. The analyzer includes a drive mechanism having a stepping motor as a power source. The drive mechanism transmits power from a rotation output shaft of the stepping motor to a moving unit for executing a target motion via at least one power transmission unit. The amount of idling of the moving unit stemming from the stepping motor getting driven in reverse is calculated from the amount of movement of the moving unit, from the amount of pulses fed to the stepping motor, and from the amount of remaining pulses so as to perform motion control of the drive mechanism accordingly.

Abstract: A fluid sample collection device for a disk-based fluid separation system is disclosed. The disk-based separation system includes a compact microfluidic disk with at least one flow channel pattern formed on a side surface of the disk. At least one orifice is formed on an outflow boundary of the disk and is designed in fluid communication with the flow channel pattern through a communication channel. The fluid sample collection device includes at least one collection tube having an open end serving as a fluid receiving end and corresponding to the orifice of the disk with a distance. When the disk is rotated, at least a portion of fluid sample in a sample processing reservoir formed on the disk is delivered by centripetal force through the communication channel and the orifice, and finally the expelling fluid sample is collected in the collection tube.

Abstract: Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.

Abstract: Fluid storage containers and analysis cartridges for use in assay processes are presented. In addition, systems comprising such storage containers and analysis cartridges and methods of using such containers and cartridges are presented as well. In specific embodiments, fluid storage containers are configured to be coupled to analysis cartridges in a first stage and a second stage.

Abstract: A microchip for measuring platelet function by allowing blood to flow through a channel to induce platelet aggregation, wherein the microchip has a channel provided inside thereof, wherein at least a part of the channel is coated with collagen for allowing adhesion of platelets; a plurality of walls extend along the direction of the flow of blood in the channel and divide the width of the channel to form a channel dividing section; and the walls are treated to have a surface roughness (Ra) of 10 to 200 nm.

Abstract: An electrically passive device and method for in-situ acoustic emission, and/or releasing, sampling and/or measuring of a fluid or various material(s) is provided. The device may provide a robust timing mechanism to release, sample and/or perform measurements on a predefined schedule, and, in various embodiments, emits an acoustic signal sequence(s) that may be used for triangulation of the device position within, for example, a hydrocarbon reservoir or a living body.

Abstract: Methods and systems for analyzing ratios of analytes within a flowing sample are provided. The flowing sample can be processed in real-time to determine a time interval over which a predetermined amount of a group of analytes passes by a fixed point in a flow cell. The predetermined amount can be routed to a sample container for future processing. The sample can comprise diluted blood and the analytes can comprise a component of hemoglobin, such as A1c, and the total amount of hemoglobin, of which the predetermined amount is metered.

Abstract: Methods and apparatus for selectively accessing a portion of a sterile cryopreserved sample are disclosed. The apparatus may include a container configured to receive the cryopreserved sample and having a first portion and a second portion, a heat sink chamber surrounding the first portion of the container, and a heat source adjacent to the second portion of the container. The chamber may be configured to maintain a non-accessed portion of the sample in a cryopreserved state. The heat source may be configured to separating an accessed portion of the sample from the non-accessed portion of the sample while maintaining the viability of the accessed portion while the non-accessed portion is maintained in the cryopreserved state.

Abstract: A method for providing a dried reagent in a microfluidic system is provided, the method comprising the following steps: providing a microfluidic system having a microfluidic structure, introducing a flowable carrier medium containing a reagent in the microfluidic structure, and drying the reagent in the microfluidic structure by lyophilization.

Abstract: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

Abstract: An automatic analyzer is capable of controlling the gap between the tip of the sample nozzle and the bottom surface of the reaction container and restricting the sample attached to the tip of the sample nozzle. A movement distance of an arm is calculated and stored while the tip of the sample nozzle contacts a coordinate measurement stand until a stop position detector detects a stop position detection plate. The sample nozzle is moved toward the bottom surface of the reaction container and is stopped at the time when the stop position detector detects the stop position detection plate. The arm is moved upwardly for the movement distance stored in memory from this position. The sample nozzle can be stopped such that the tip of the sample nozzle is contacted to the bottom surface of the reaction container and the bend (warp) of the sample nozzle is restricted.

Abstract: An object is to provide a micro plate treating device and micro plate treating method capable of handling a larger number of kinds of solution or suspensions or a larger volume of solutions or suspensions per work area by use of a normalized micro plate without enlarging the scale of the device.

Abstract: A slide processing apparatus is disclosed. The apparatus comprise a liquid transporter for transporting a staining liquid reserved in a first liquid container and a washing liquid reserved in a second liquid container; a first instruction receiver for receiving a first instruction that instructs to initiate preparation of a slide stained with the staining liquid; a second instruction receiver for receiving a second instruction that instructs to initiate washing of a housing element which accommodates therein a slide to be stained; and a controller. When receiving the first instruction, the controller performs staining of the slide accommodated in the housing element by causing the liquid transporter to transport the staining liquid into the housing element. When receiving the second instruction, the controller performs washing of the housing element by causing the liquid transport to transport the washing liquid into the housing element.

Abstract: A microfluidic processing unit for the automated processing of liquid samples and method thereof are disclosed. In one embodiment, the unit comprises first and second main channels connected to a pump for generating a negative or positive pressure therein; a sample channel which supplies a liquid sample is connectable to a sample vessel containing the liquid sample, the sample channel communicates with the first main channel and is equipped with an on/off valve; one or more reagent channels for supplying one or more reagents, the reagent channels being connectable to reagent vessels containing reagents for reacting with the liquid samples, each of the reagent channels communicating with the first or second main channel and being equipped with an on/off valve; and a reaction chamber for reacting a sample and reagent, the reaction chamber being connected to the first and second main channels via at least one on/off valve.

Abstract: A delivery apparatus for selectively delivering one or more liquid reagents into a reaction or test chamber (2), especially of an assay apparatus, the apparatus comprising: one or more respective storage chambers (5,6) for containing the one or more liquid reagents and arranged generally above the reaction or test chamber (2); and a plunger element (4) arranged and operable for insertion into the mouth of a selected storage chamber so as to displace a selected reagent from therewithin into the reaction or test chamber (2) generally therebelow by gravitational liquid overflow from the mouth of the chamber. The apparatus may conveniently be provided as a discrete delivery unit, with the storage chambers (5,6) prefilled with the selected reagents.

Abstract: Devices and methods use an integrated microfluidic system that has the capability of realizing a wide range of accurate dilutions in a logarithmic way through semi-direct dilution of samples inside a chip. The device for dose response analysis is able to contain a first fluid source on a microfluidic chip, wherein the first fluid source comprises a drug, a second fluid source on the microfluidic chip, a mixing area on the microfluidic chip fluidically coupling with the first and the second fluidic source, and a detection area coupling with the mixing area for drug information detection using a detection system.

Abstract: A module for processing a biological sample for an analysis test is disclosed. The processing module includes, in at least one embodiment, an interface at which the processing module can be connected to a cartridge with a lab-on-a-chip, in which cartridge the analysis steps are carried out. A biochip kit is also disclosed. In at least one embodiment, the biochip kit includes one or more processing modules which are intended for different sample materials and which can be connected to the same cartridge type.

Abstract: Microfluidic structures and methods for manipulating fluids and reactions are provided. Such structures and methods may involve positioning fluid samples, e.g., in the form of droplets, in a carrier fluid (e.g., an oil, which may be immiscible with the fluid sample) in predetermined regions in a microfluidic network. In some embodiments, positioning of the droplets can take place in the order in which they are introduced into the microfluidic network (e.g., sequentially) without significant physical contact between the droplets. Because of the little or no contact between the droplets, there may be little or no coalescence between the droplets. Accordingly, in some such embodiments, surfactants are not required in either the fluid sample or the carrier fluid to prevent coalescence of the droplets. Structures and methods described herein also enable droplets to be removed sequentially from the predetermined regions.