Magnetic Patents (Class 436/526)
  • Patent number: 11883679
    Abstract: The disclosure provides systems for ultrasound-induced thrombolysis with magnetic microbubbles under a rotational/alternating magnetic field, sonothrombolysis systems with magnetic microbubbles and optional nanodroplets for inducing thrombolysis under an acoustic field, and a rotational/alternating magnetic field, and methods of treating patients with blood clots using the sonothrombolysis systems of the present disclosure.
    Type: Grant
    Filed: October 12, 2020
    Date of Patent: January 30, 2024
    Assignee: North Carolina State University
    Inventors: Xiaoning Jiang, Bohua Zhang
  • Patent number: 11857981
    Abstract: A magnetic separator is disclosed. The magnetic separator comprises an array of magnets configured to interact with a tube holder plate, wherein the tube holder plate comprises an array of tubes. The magnetic separator comprises a raised frame extending around a periphery of the array of magnets such that the raised frame is configured to support the tube holder plate such that the array of tubes is suspended above the array of magnets.
    Type: Grant
    Filed: March 30, 2020
    Date of Patent: January 2, 2024
    Assignee: 10x Genomics, Inc.
    Inventors: Pratomo Alimsijah, John Richard Chevillet
  • Patent number: 11845772
    Abstract: Disclosed herein are methods for purifying RNA comprising poly A. Also disclosed herein are compositions such as surfaces and oligonucleotides for purifying RNA comprising polyA. Other embodiments are also disclosed. Commercially-available resins having polythymidine oligonucleotide ligands typically contain less than 30 thymidine (2?deoxy) residues and some commercial resin suppliers utilize a distribution of dT chain lengths, not of a discreet length.
    Type: Grant
    Filed: June 30, 2022
    Date of Patent: December 19, 2023
    Assignee: ModernaTX, Inc.
    Inventors: William Joseph Issa, John Grant Aunins, Stephane Bancel
  • Patent number: 11796498
    Abstract: An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.
    Type: Grant
    Filed: June 30, 2020
    Date of Patent: October 24, 2023
    Assignee: Altratech Limited
    Inventors: Timothy Cummins, Brian O'Farrell
  • Patent number: 11781183
    Abstract: Methods of determining the origin of cell free DNA (cfDNA), for detecting death of a cell type or tissue in a subject, for determining a cellular state of a cell as it died, and combinations thereof, are provided. As are computer program products for doing same.
    Type: Grant
    Filed: March 13, 2019
    Date of Patent: October 10, 2023
    Inventors: Ronen Sadeh, Nir Friedman, Alon Appleboim
  • Patent number: 11774444
    Abstract: Methods and apparatus for detecting cell-bound and soluble antigens in a biological sample are described. The method comprises forming complexes of at least one antibody-coated bead and at least one antigen in a solution, placing the solution in a magnetic field such that the formed complexes levitate in the solution at a particular height, and determining at least one characteristic of the antigen in the complexes based, at least in part, on an image of the complexes showing the magnetic levitation height.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: October 3, 2023
    Assignee: Beth Israel Deaconess Medical Center, Inc.
    Inventors: Ionita C. Ghiran, Nathan Shapiro, Anish V. Sharda, Edward J. Felton
  • Patent number: 11761972
    Abstract: The objective of the present disclosure is to provide a technique for reducing a quantity of magnetic particles remaining on a reaction vessel wall surface in a cleaning step for reducing, in a stepwise manner, an amount of a magnetic particle solution in the reaction vessel. The automated analysis device according to the present disclosure causes an agitating mechanism to operate in such a way that a magnetic substance remaining on the wall surface of the vessel in the previous cleaning step is captured by a cleaning solution in the next cleaning step.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: September 19, 2023
    Assignee: Hitachi High-Tech Corporation
    Inventors: Tooru Inaba, Ruochi Hsu, Shinya Matsuoka, Takeshi Yokokawa, Daisuke Ebihara
  • Patent number: 11754648
    Abstract: A significant enhancement of detection capabilities of the room temperature MPQ is seen using optical lithography-defined, ferromagnetic iron-nickel alloy microdisks. Irreversible transitions between strongly non-collinear (vortex) and a collinear single domain states, driven by an ac magnetic field, translate into a nonlinear magnetic response that enables ultrasensitive detection of material at relatively small magnetic fields.
    Type: Grant
    Filed: January 25, 2021
    Date of Patent: September 12, 2023
    Assignee: UChicago Argonne, LLC
    Inventors: Valentine Novosad, Elena A. Rozhkova, Maxim Nikitin, Petr Nikitin
  • Patent number: 11747329
    Abstract: Disclosed herein are devices for molecule detection and methods for using detection devices for molecule detection, such as nucleic acid sequencing. In some embodiments, a detection device comprises one or more pole pieces, one or more sensors, each of the one or more sensors coupled to at least one of the one or more pole pieces, and detection circuitry coupled to the one or more sensors. The detection circuitry is configured to detect a characteristic of each of the one or more sensors, the characteristic indicating presence or absence of one or more magnetic nanoparticles (MNPs) coupled to at least one of a plurality of molecules to be detected, and at least one of the one or more pole pieces is operable to draw the one or more MNPs toward at least one of the one or more sensors.
    Type: Grant
    Filed: March 19, 2020
    Date of Patent: September 5, 2023
    Assignee: Western Digital Technologies, Inc.
    Inventor: Daniel Bedau
  • Patent number: 11713481
    Abstract: A process for pre-concentration and detection of at least one single-stranded nucleic acid target molecule, the process comprising the steps of generating a flow of liquid comprising at least one magnetic nanoparticle in a micro-channel and a plurality of single-stranded nucleic acid probe molecules attached to the nanoparticle, generating an alternating magnetic field in the part of the micro-channel using an electromagnet, the magnetic field having an intensity and frequency that are suitable for causing magnetic hyperthermia of the nanoparticles so as to cause denaturing of the duplex formed by the single-stranded nucleic acid target molecule and the single-stranded nucleic acid probe molecule, and detecting the single-stranded target molecule dispersed in the liquid.
    Type: Grant
    Filed: October 5, 2018
    Date of Patent: August 1, 2023
    Assignees: Centre National de la Recherche Scientifique (CNRS), Institut National de La Sante et de la Recherche Medicale (INSERM), Sorbonne Universite
    Inventors: Marie-Charlotte Horny, Jean-Michel Siaugue, Vincent Dupuis, Mathieu Lazerges, Jean Gamby
  • Patent number: 11634703
    Abstract: Embodiments of the system and/or method can include and/or apply a magnetic device for facilitating a magnetic field for isolating the nucleic acid material from a sample, the magnetic device including a support component; and a set of magnetic pins attached to the support component and movable with at least three degrees of freedom when attached to the support component, where the set of magnetic pins provide adaptability to shapes of sample compartments of a sample container.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: April 25, 2023
    Assignee: Psomagen, Inc.
    Inventors: Zachary Apte, Jessica Richman, Daniel Almonacid, Eduardo Morales, Constance Norris, Nicolas Ordenes, Pamela Nieto
  • Patent number: 11619629
    Abstract: Provided in the present invention are a magnetic nanosphere coated with modified cardiolipin, and manufacturing method thereof. The magnetic nanosphere coated with modified cardiolipin comprises a modified cardiolipin, a biotin derivative, and a streptavidin magnetic bead. The modified cardiolipin is coupled to the biotin derivative via an —NH—CO structure. The streptavidin magnetic bead is a magnetic nanosphere coupled to streptavidin, and the biotin derivative is coupled to the streptavidin.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: April 4, 2023
    Inventors: Fuzhen Xia, Dingbiao Zou, Gang Wang, Xiaoqin Ye, Chungen Qian
  • Patent number: 11591648
    Abstract: Method of utilizing a nanochannel in combination with at least one magnetic sensor for detecting (e.g., identifying) molecules, cells, and other analytes. Particularly, the method includes bringing molecules, labeled with magnetic nanoparticles (MNPs), in close proximity to the magnetic sensor to identify the molecules via an output signal from the magnetic sensor. The method is particularly suited for identifying nucleotides of DNA and RNA strands.
    Type: Grant
    Filed: October 2, 2019
    Date of Patent: February 28, 2023
    Inventors: Gemma Mendonsa, Riyan A. Mendonsa, Krishnan Subramanian
  • Patent number: 11561227
    Abstract: The present disclosure relates to a Proteomics-to-Genomics approach allows for in silico validation of biomarkers and drug targets. Biomarkers having high prognostic value in predicting cancer patient populations that may benefit from mitochondrial biogenesis inhibitor therapy may be identified under the present approach. Also disclosed are methods for identifying candidates for anti-mitochondrial therapy, and in particular mitochondrial biogenesis inhibitor therapy. Diagnostic kits including reagents for determining transcripts or probes of high prognostic value are also disclosed. Additionally, mitochondrial biogenesis inhibitors may be used as anti-cancer agents for diverse oncogenic stimuli, including for example, c-MYC and H-Ras oncogenes, as well as environmental stimuli such as, for example rotenone.
    Type: Grant
    Filed: October 11, 2018
    Date of Patent: January 24, 2023
    Inventors: Michael P. Lisanti, Federica Sotgia
  • Patent number: 11541391
    Abstract: The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation.
    Type: Grant
    Filed: September 9, 2019
    Date of Patent: January 3, 2023
    Assignee: Applied Cells Inc.
    Inventor: Yuchen Zhou
  • Patent number: 11536658
    Abstract: The present invention provides a method for determining concentration of polyelectrolyte or phosphonate in a sample comprising polyelectrolyte or phosphonate in low concentrations. The method comprises admixing the sample with a reagent comprising a lanthanid(lll) ion; admixing the sample with silica; allowing the polyelectrolyte or phosphonate in the sample to interact with the reagent comprising the lanthanide(lll) ion and the silica; exciting the sample and detecting a sample signal deriving from the lanthanide(lll) ion by time-resolved fluorescence measurement; and determining the concentration of the polyelectrolyte or phosphonate in the sample by using the detected sample signal.
    Type: Grant
    Filed: September 27, 2019
    Date of Patent: December 27, 2022
    Assignee: Kemira Oyj
    Inventors: Salla Puupponen, Sari Krapu
  • Patent number: 11525827
    Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: December 13, 2022
    Inventors: Vamsee K. Pamula, Michael G. Pollack, Ramakrishna Sista, Arjun Sudarsan
  • Patent number: 11513089
    Abstract: A system for measuring electrical characteristics of bioparticles is described. The system comprises an incubator for performing electrochemical measurements in a defined environment and a substrate holder positioned in said incubator for holding a substrate comprising a plurality of wells. The system is furthermore configured for continuously or regularly measuring electrochemical data. The system also comprises a processing means for comparing the continuously or regularly measured electrochemical data with reference data and for determining a moment for adding an active compound based on said comparison.
    Type: Grant
    Filed: December 18, 2016
    Date of Patent: November 29, 2022
    Inventors: Yves Van Ingelgem, Bart Landuyt, Sven Verguts
  • Patent number: 11446668
    Abstract: An automatic analysis device which can be accessed from a front surface of the device to a rear surface side of the device when a user accesses the automatic analysis device and reduce the risk of damage to a rod-shaped member due to contact. A reagent suction position, a reagent discharge position, a reagent dispensing nozzle cleaning portion, and a reagent dispensing mechanism retraction portion from which the reagent dispensing mechanism is retracted are disposed on a trajectory of an arm of the reagent dispensing mechanism. A nozzle guide accommodation portion accommodates a nozzle guide of a reagent dispensing mechanism and the reagent dispensing nozzle. The reagent dispensing mechanism retraction portion is a cylindrical member protruding downward from an upper surface cover of the automatic analysis device and prevents direct contact with the reagent dispensing nozzle by positioning the reagent dispensing nozzle in the nozzle accommodation portion.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: September 20, 2022
    Inventors: Shunsuke Sasaki, Takenori Okusa
  • Patent number: 11408854
    Abstract: A diagnostic device is described herein that can be used to perform magneto-optical detection and discernment of crystals within a biofluid sample. A magnetic field can be applied by the diagnostic device in a direction relative to light traveling through the sample. The presence of a crystal can be determined based on the magneto-optical properties of the sample. The detected crystal can be one of two similar crystal types that may be in the biofluid sample. The two similar crystal types can exhibit different magneto-optical properties under a magnetic field in a different direction. Accordingly, the type of crystal can be discerned by applying the magnetic field in the different direction as light travels through the sample. Discernment of the type of crystal can lead to diagnosis of the particular disease condition and subsequent proper treatment of the disease condition.
    Type: Grant
    Filed: August 25, 2017
    Date of Patent: August 9, 2022
    Inventors: Tanvir Baig, Robert Brown, Robert Deissler, Brian T. Grimberg, Abdullah Amin
  • Patent number: 11385224
    Abstract: Systems and methods for use in an in vitro diagnostics setting incorporating magnetic shielding to reduce exposure of any of samples, reactants, devices or people from exposure or prolonged exposure to magnetic or electromagnetic fields generated with the system. Some embodiments provide an automation system for use in an in vitro diagnostics setting comprising an automation track having a sidewall provided with magnetic shielding. In some embodiments, the magnetic shielding comprises an 80% nickel alloy.
    Type: Grant
    Filed: July 19, 2017
    Date of Patent: July 12, 2022
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Kung-Le Chang, Manuel Lavin, Baris Yagci
  • Patent number: 11361886
    Abstract: The present invention relates to magnetic single-core nanoparticles, in particular stable dispersible magnetic single-core nanoparticles (e.g. single-core magnetite nanoparticles) having a diameter between 20 and 200 nm in varied morphology, and the continuous aqueous synthesis thereof, in particular using micromixers. The method is simple, quick and cost-effective to perform and is carried out without organic solvents. The single-core nanoparticles produced by the method form stable dispersions in aqueous media, i.e. not having a tendency to assemble or aggregate. In addition, the method offers the possibility of producing anisotropic, super-paramagnetic, plate-shaped nanoparticles which, due to their shape anisotrophy, are extremely suitable for use in polymer matrices for magnet field-controlled release of active substances.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: June 14, 2022
    Assignee: Fraunhofer-Gesellschaft zurförderung der angewandten Forschung e.V.
    Inventors: Regina Bleul, Raphael Thiermann
  • Patent number: 11294004
    Abstract: Disclosed is a system for detecting one or more target analytes which includes a resistor structure comprised of a substrate, a graphene-based nanocomposite material located on a surface of the substrate with the graphene-based nanocomposite material exhibiting one or more magnetoresistance properties. A surface of the nanocomposite material includes molecular sensing elements bound thereto which exhibit an affinity for binding with the target analytes. Electrodes are connected to the resistor structure connectable to a power source and a device for measuring a resistance across the resistor structure for sensing a giant magnetoresistance (GMR) value of the resistor structure. Included are magnetic colloidal nanoparticles exhibiting preselected magnetic properties with an outer surface of the magnetic colloidal nanoparticles being modified to allow interaction with the surface of the resistor structure resulting in a change in the GMR value of the resistor structure.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: April 5, 2022
    Inventor: Jin Zhang
  • Patent number: 11273439
    Abstract: A system and method for target material retrieval and processing, the system comprising: an adaptor configured to interface with a capture region of a capture substrate for capturing particles in single-particle format within a set of wells, wherein the adaptor comprises a first region configured to interface with the capture region, a second region, and a cavity extending from the first region to the second region; and a support structure coupled to the adaptor and providing a set of operation modes for movement of the adaptor relative to the capture substrate. The system enables methods for magnetic and/or other force-based methods of retrieval of target material (e.g., derived from single cells).
    Type: Grant
    Filed: June 19, 2020
    Date of Patent: March 15, 2022
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Swati Ranade, Vishal Sharma, Austin Payne, Sam Tuck
  • Patent number: 11268056
    Abstract: Methods of separating magnetic particles from a fluid include introducing a fluid mixture that includes a liquid media, a biological component, and magnetic particles into an internal compartment of a container through an inlet, allowing at least a portion of the mixture to travel around a partition formed within the internal compartment and then exit the internal compartment through an outlet on the opposite side of the partition, and applying a magnetic field to the mixture in the internal compartment so that the magnetic particles are retained within the container or internal compartment thereof by the magnetic field. The partition is formed by permanently or reversibly securing upper and lower container walls together, such as by welding or pressing, between the inlet and the outlet such that the media and biological component must flow around the partition and through the magnetic field to exit the internal compartment through the outlet.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: March 8, 2022
    Assignees: Life Technologies Corporation, Life Technologies AS
    Inventors: Lars Norderhaugh, Angel Varela-Rohena, Nephi D. Jones
  • Patent number: 11173488
    Abstract: Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: November 16, 2021
    Assignee: Purdue Research Foundation
    Inventors: Cagri A. Savran, Chun-Li Chang, Wanfeng Huang, Onur Gur, Rohil Jain
  • Patent number: 11155773
    Abstract: Disclosed are an apparatus and methods for rapid amplification of nucleic acids. More particularly, the present disclosure relates to an apparatus for mixing a reaction solution during amplification of nucleic acids and to methods for amplifying nucleic acids. Also disclosed are methods for lysing cells in a sample and amplifying nucleic acids.
    Type: Grant
    Filed: January 24, 2017
    Date of Patent: October 26, 2021
    Assignee: NUtech Ventures
    Inventor: Hendrik Viljoen
  • Patent number: 11143652
    Abstract: The present disclosure relates to, a magnetic microbeads adsorption mechanism, which is configured to adsorb a magnetic microbeads in a reaction cup and is provided with a cup inlet station and a cup outlet station; the magnetic microbeads adsorption mechanism includes a pedestal, a turntable and multiple magnetic adsorption components; the turntable is rotatably mounted on the pedestal; the turntable can drive the reaction cup to rotate around a central axis of the turntable; the multiple magnetic adsorption components are arranged on a mounting circumference of the pedestal at intervals; the mounting circumference and rotation track of the reaction cup are concentrically arranged; and during a process when the reaction cup rotates from the cup inlet station to the cup outlet station, the adsorption height of the magnetic microbeads relative to cup bottom of the reaction cup can be changed.
    Type: Grant
    Filed: January 21, 2019
    Date of Patent: October 12, 2021
    Assignee: Shenzhen New Industries Biomedical Engineering Co., Ltd.
    Inventors: Liang Zhu, Li Yin, Yi Hu, Dingping Ban, Wanguan Yi, Shuai Tong
  • Patent number: 11054416
    Abstract: The present disclosure is directed towards characterizing liquids through the use of magnetic discs that rotate in response to dynamic magnetic fields. In some embodiments, a light beam is transmitted into the liquid while the magnetic discs rotate, and one or more parameters of a light beam signal associated with the transmitted light beam are identified. Various characteristics of the liquid may be detected based on the one or more parameters of the light beam signal.
    Type: Grant
    Filed: January 11, 2018
    Date of Patent: July 6, 2021
    Assignee: University of Florida Research Foundation
    Inventors: David P. Arnold, Nicolas Garraud
  • Patent number: 11035869
    Abstract: Disclosed is an automated liquid-phase immunoassay apparatus used with a cuvette having a plurality of chambers containing a reagent necessary for detection of an analyte in a biological specimen. The apparatus includes a movable cuvette module equipped with the cuvette, an optical reading module for optical assaying of a material resulting from a reaction between the specimen and the reagent, and a dispenser module which is positioned on the cuvette module and which dispenses the specimen and the reagent to the plurality of chambers of the cuvette and washes the specimen and the reagent therefrom.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: June 15, 2021
    Assignee: BODITECH MED INC.
    Inventors: Eui Yul Choi, Hoo Don Joo, Hyung Hoon Kim, Chuhyun Cho, Uk Bin Im, Young Jin Oh, Youn Tae Im, Ji Woon Jung
  • Patent number: 11007285
    Abstract: The present disclosure relates to a composition of albumin microbubbles to which are bound one or more moieties that exhibit a binding preference for the albumin microbubbles relative to free, native HSA. Production of the albumin microbubble composition and use of the albumin microbubble composition in ultrasound mediated delivery of therapeutic or diagnostic agents is also discussed.
    Type: Grant
    Filed: July 22, 2020
    Date of Patent: May 18, 2021
    Inventors: Matthew David Butts, Jason William Castle, Andrew Soliz Torres, Jeannette Christine Roberts, Binil Itty Ipe Kandapallil
  • Patent number: 10901051
    Abstract: A significant enhancement of detection capabilities of the room temperature MPQ is seen using optical lithography-defined, ferromagnetic iron-nickel alloy microdisks. Irreversible transitions between strongly non-collinear (vortex) and a collinear single domain states, driven by an ac magnetic field, translate into a nonlinear magnetic response that enables ultrasensitive detection of material at relatively small magnetic fields.
    Type: Grant
    Filed: August 15, 2017
    Date of Patent: January 26, 2021
    Assignee: UChicago Argonne, LLC
    Inventors: Valentine Novosad, Elena A. Rozhkova, Maxim Nikitin, Petr Nikitin
  • Patent number: 10799863
    Abstract: A chemical sensor that enables high detection accuracy. A chemical sensor includes a substrate, a magnetoresistance-effect element disposed over the substrate, a first film disposed over the magnetoresistance-effect element, and a second film disposed in a region in the vicinity of the magnetoresistance-effect element or over a region in the vicinity of the magnetoresistance-effect element, wherein the second film has higher solubility in a specific liquid than the first film.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: October 13, 2020
    Inventors: Sachio Tsuboike, Susumu Haratani, Sumiko Kitagawa, Takashi Kikukawa, Haruki Yuga
  • Patent number: 10782232
    Abstract: A Quality Control (QC) material for performing a QC procedure with respect to at least one detector is introduced. The QC material comprises at least one first QC substance and at least one second QC substance, wherein the first QC substance is interferable with the second QC substance or with the performance and/or lifetime of the detector and wherein the first QC substance is entrapped by carrier particles that prevent the first QC substance to interfere with the second QC substance or with the performance and/or lifetime of the detector. An in-vitro diagnostic system comprising a first detector and a second detector and the QC material is also introduced. An in-vitro diagnostic method comprising performing a QC procedure with respect to a first detector and/or to a second detector comprising providing the QC material is also introduced, as well as a method of manufacturing the QC material.
    Type: Grant
    Filed: September 21, 2017
    Date of Patent: September 22, 2020
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Sabrina Stevanovic, Rebecca Ravotti
  • Patent number: 10684280
    Abstract: A toner composite material includes toner particles that include a sulfonated polyester and a wax and metal nanoparticles disposed on the surface of the toner particles. A method includes providing such toner composite materials, fusing the material to a substrate and covalently linking a ligand to the surface of the silver nanoparticles via a thiol, carboxylate, or amine functional group. Detection strips include a substrate and such toner composite materials fused on the substrate.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: June 16, 2020
    Inventors: Valerie M. Farrugia, Wendy Chi, Sandra J. Gardner
  • Patent number: 10557174
    Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: February 11, 2020
    Assignee: Life Technologies Corporation
    Inventors: Gordon A. Janaway, Mark Andersen, Kornelija Zgonc, Michael C. Pallas, Marcin Sikora, Casey R. McFarland, Ferrier N. Le, Haopeng Wang, Jian Gong, Gothami Padmabandu
  • Patent number: 10488408
    Abstract: The application relates to a method for detecting a target in a sample suspected of containing the target. The method comprises contacting the sample and a first binding molecule attached to a magnetic particle with a second binding molecule attached to a solid support. The first binding molecule is capable of binding to the second binding molecule, and the target is capable of interfering with this binding. Magnetic force is applied to bring the magnetic particle into close proximity with the solid support. The number of magnetic particles bound to the solid support is detected.
    Type: Grant
    Filed: April 27, 2007
    Date of Patent: November 26, 2019
    Inventors: Thea Van Der Wijk, Eduard Gerard Marie Pelssers, Jessica Amadio
  • Patent number: 10451599
    Abstract: A method of detecting a metal ion in a protein-containing sample includes adding a protein degrading enzyme to the protein-containing sample to form an enzyme degradation product, adding an acid to the enzyme degradation product to provide a mixture, filtering the mixture to provide a supernatant, extracting the supernatant with an organic solvent to remove organic solvent soluble byproducts to provide a washed aqueous layer, and detecting the metal ion in the washed aqueous layer. The method is amenable to the detection of heavy metal ions in complex products such as milk. A kit includes reagents for performing the method.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: October 22, 2019
    Inventors: Joy Sinha, Gerson Aguirre
  • Patent number: 10444240
    Abstract: A ratio of rare cells to non-rare cells in a blood sample suspected of containing rare cells and non-rare cells is enhanced. A treated blood sample is prepared by providing in combination the blood sample, a platelet deactivation agent, a fibrin-formation-arresting agent and fibrin in an amount sufficient to cause a predetermined level of agglutination of the rare cells. The treated blood sample is then contacted with a porous matrix such that agglutinated rare cells are preferentially retained on the porous matrix. The rare cells may then be identified.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: October 15, 2019
    Inventor: Michael Pugia
  • Patent number: 10422796
    Abstract: A method for measuring fibroblast growth factor-23 (FGF-23) in a sample, which comprise the following steps: (1) reacting, in an aqueous medium, FGF-23 in a sample with magnetic particles, a first antibody or a fragment thereof which binds to FGF-23, and a second antibody or a fragment thereof which binds to FGF-23, to form on the magnetic particles an immunocomplex comprising the first antibody or a fragment thereof which binds to FGF-23, FGF-23, and the second antibody or a fragment thereof which binds to FGF-23; (2) collecting the magnetic particles in the reaction mixture after step (1) by magnetic force, and separating the magnetic particles collected by magnetic force from the other components; and (3) measuring the immunocomplex on the magnetic particles separated in step (2). The present invention provides a method for measuring FGF-23 in a sample, which have a high sensitivity and have a wide measurement range.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: September 24, 2019
    Assignee: KYOWA MEDEX CO., LTD.
    Inventors: Koji Uzawa, Emiko Suzuki, Kazuyuki Ikeda, Kazuki Morita
  • Patent number: 10384203
    Abstract: The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.
    Type: Grant
    Filed: September 24, 2009
    Date of Patent: August 20, 2019
    Assignee: First Light Biosciences, Inc.
    Inventors: Greg Yantz, Don Straus, Gordon Siek, Damon DeHart
  • Patent number: 10352932
    Abstract: In one aspect, presence and/or level of an analyte within a sample is determined by use of a construct comprising a magnetic moiety and a fluorescent moiety. In one embodiment, the construct is magnetically migrated to a transparent surface and then dragged along the surface. In one aspect, an evanescent field is applied and changes in the diffusional or rotational properties of the fluorescent moiety as it migrates in and out of the evanescent field are measured by changes in its fluorescent emission, providing a measure of the interaction between the construct and a component of the sample.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: July 16, 2019
    Inventor: Christopher Gordon Atwood
  • Patent number: 10261076
    Abstract: Small molecule analytes (less than 1000 Daltons) in a fluid sample are detected using a competitive assay in a magnetic biosensor. The fluid sample is added to a biosensor detection chamber together with detection probes and magnetic tags which bind to the detection probes. The magnetic biosensor is functionalized with a capture probe that shares an epitope with the analytes, and the detection probe is capable of binding the epitope shared by the analytes and the capture probe, so that the presence of the analyte prevents detection probes (and magnetic tags) from binding to the biosensor. By measuring the binding of the magnetic tags to the magnetic biosensor, an amount of analytes in the solution is determined.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: April 16, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Tyler O'Brien Shultz, Jung-Rok Lee, Shan X. Wang
  • Patent number: 10240186
    Abstract: Methods, microfluidic devices, and instruments for magnetic separation of particles from a fluid are described. Examples include microfluidic devices having a removable portion. Examples include microfluidic devices having one or more regions of reduced fluid velocity. Examples further including instruments having pneumatic interfaces. Examples further includes instruments having controllable magnets, imaging components, or combinations thereof.
    Type: Grant
    Filed: July 14, 2011
    Date of Patent: March 26, 2019
    Inventors: Cristian Ionescu-Zanetti, Joshua Tanner Nevill, Michael Schwartz, Carolyn G. Conant, Roger Rudoff
  • Patent number: 10203326
    Abstract: A method of detecting a target substance, containing the steps of: incorporating labeling particles into a test liquid containing an analyte; heating the test liquid; irradiating the test liquid with excitation light, and detecting the target substance contained in the test liquid depending on a state of light emission of the labeling particles; wherein an aggregation state of the labeling particles is changed by the heating step; and wherein the labeling particles have a thermoresponsive polymer on a surface of a composite particle containing a magnetic material and a fluorescent material, and further have a biomolecule having properties of binding with the target substance.
    Type: Grant
    Filed: April 1, 2016
    Date of Patent: February 12, 2019
    Inventors: Masayuki Fukushima, Michio Ohkubo, Kazutomi Miyoshi, Masataka Nishida
  • Patent number: 9913917
    Abstract: The invention relates to highly fluorescent metal oxide nanoparticles to which biomolecules and other compounds can be chemically linked to form biocompatible, stable optical imaging agents for in vitro and in vivo applications. The fluorescent metal oxide nanoparticles may also be used for magnetic resonance imaging (MRI), thus providing a multi modality imaging agent.
    Type: Grant
    Filed: December 22, 2006
    Date of Patent: March 13, 2018
    Assignee: VisEn Medical, Inc.
    Inventors: Kevin Groves, Milind Rajopadhye
  • Patent number: 9878327
    Abstract: This disclosure describes microfluidic devices that include one or more magnets, each magnet being operable to emit a magnetic field; and a magnetizable layer adjacent to the one or more magnets, in which the magnetizable layer is configured to induce a gradient in the magnetic field of at least one of the magnets. For example, the gradient can be at least 103 T/m at a position that is at least 20 ?m away from a surface of the magnetizable layer. The magnetizable layer includes a first high magnetic permeability material and a low magnetic permeability material arranged adjacent to the high magnetic permeability material. The devices also include a microfluidic channel arranged on a surface of the magnetizable layer, wherein a central longitudinal axis of the microfluidic channel is arranged at an angle to or laterally offset from an interface between the high magnetic permeability material and the low magnetic permeability material.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: January 30, 2018
    Assignee: The General Hospital Corporation
    Inventors: Kyle C. Smith, Ramin Haghgooie, Thomas Alan Barber, Ismail Emre Ozkumur, Ravi Kapur, Mehmet Toner
  • Patent number: 9869674
    Abstract: The invention relates to a method for determining the presence of an analyte by means of a distribution of small magnetic particles. According to said method, the magnetizations of the small particles are oriented in relation to each other by means of an outer magnetic focusing field; once the focussing field has been terminated, the magnetizations of the small particles are rotated asynchronously to the magnetic field by means of an outer magnetic field of suitable field intensity and rotational frequency, which rotates about a longitudinal axis (z); the temporal course of the superpositioned transverse magnetization of the set of particles is detected; and the presence of the analyte is deduced from the detected temporal course. The invention also relates to a corresponding device (1).
    Type: Grant
    Filed: April 1, 2011
    Date of Patent: January 16, 2018
    Assignees: Hochschule für angewandte Wissenschaften Fachhochschule Würzburg-Schweinfurt, Julius-Maximilians-Universität-Würzburg
    Inventors: Martin Rueckert, Volker C. Behr, Thomas Kampf
  • Patent number: 9851308
    Abstract: Methods of detecting very low levels of targets, such as cells, are provided. In some embodiments, for example, the methods can detect bacteria present in a sample at concentrations less than 25 cells/mL. The method involves detecting nanoparticle aggregation in the absence of the target.
    Type: Grant
    Filed: November 18, 2011
    Date of Patent: December 26, 2017
    Assignee: Wisconsin Alumni Research Foundation (WARF)
    Inventors: Sundaram Gunasekaran, Seok won Lim
  • Patent number: 9823316
    Abstract: A magnetic biosensor can include a magnetic stack comprising a free layer, a fixed layer, and a nonmagnetic layer between the free layer and the fixed layer. At least one of the free layer or the fixed layer may have a magnetic moment oriented out of a major plane of the free layer or the fixed layer, respectively, in an absence of an external magnetic field. The magnetic biosensor also may include a sample container disposed over the magnetic stack, a plurality of capture antibodies attached to a bottom surface of the sample container above the magnetic stack, and a magnetic field generator configured to generate a magnetic field substantially perpendicular to the major plane of the free layer or fixed layer.
    Type: Grant
    Filed: October 19, 2012
    Date of Patent: November 21, 2017
    Inventors: Jian-Ping Wang, Md Tofizur Rahman, Yi Wang