Polysaccharide, Carrier (e.g., Dextran, Etc.) Patents (Class 436/529)
  • Patent number: 10436684
    Abstract: Aspects of the present disclosure include a solid phase sorbent for preparation of analytical samples. The solid phase sorbent includes particles that are surface modified with an ?-cyclodextrin moiety. Also provided is a method of reducing matrix effects in an analytical sample. In some embodiments, the method includes contacting a sample comprising a matrix-interfering agent and an analyte with ?-cyclodextrin modified particles to produce a contacted sample wherein the matrix-interfering agent binds to the ?-cyclodextrin modified particles; separating the ?-cyclodextrin modified particles from the contacted sample to produce a matrix-reduced composition; and detecting the analyte in the matrix-reduced composition. Systems for practicing the subject methods are provided that include the subject solid phase sorbent.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: October 8, 2019
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Bruce Richter, Derick Lucas, David Long, Limian Zhao
  • Patent number: 10131714
    Abstract: The invention provides a two-step chromatography process for small and large-scale purification of proteins, specifically monoclonal antibodies, using only four buffer solutions made from a mother solution.
    Type: Grant
    Filed: May 23, 2012
    Date of Patent: November 20, 2018
    Assignee: SANOFI
    Inventors: Didier Duthe, Laure Landric-Burtin, Benoit Mothes
  • Patent number: 10032560
    Abstract: A method of manufacturing a variable capacitor includes forming a capacitor conductor. The method also includes forming a phase change material adjacent the capacitor conductor. The method further includes forming a first contact on the capacitor conductor. The method additionally includes forming a second contact and a third contact on the phase change material.
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: July 24, 2018
    Assignee: Western Digital Technologies, Inc.
    Inventor: Mark C. Lamorey
  • Patent number: 9116155
    Abstract: The invention relates to methods for the early diagnosis of Acute Coronary Syndrome.
    Type: Grant
    Filed: June 10, 2009
    Date of Patent: August 25, 2015
    Assignee: University of Massachusetts
    Inventors: Charles R. Kiefer, L. Michael Snyder, Rachel E. Stock
  • Patent number: 9040309
    Abstract: The invention provides a binding layer comprising a polysaccharide substituted by carboxylic groups or derivatives thereof exhibiting high performance in the binding of ligand molecules and in the interaction thereof with analyte molecules. A method for the preparation of the binding layer and for the assaying of various analyte molecules is also provided.
    Type: Grant
    Filed: June 21, 2006
    Date of Patent: May 26, 2015
    Assignee: BIO-RAD HAIFA LTD.
    Inventor: Shai Nimri
  • Patent number: 9023660
    Abstract: Methods are disclosed for producing a bioweapon-sensitive fibrous-network product, wherein the subject products exhibit a color change in response to exposure to a biological agent (or portion thereof) as used in a biological weapon. Also disclosed are fibrous-network products that contain units of biopolymeric material that impart a color change to the products in response to exposure to a biological agent (or portion thereof) as used in a biological weapon.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: May 5, 2015
    Inventors: Dennis Farwell, Keith Baumann
  • Patent number: 8906703
    Abstract: A method for immobilizing an amino-containing material to a substrate is described. The method involves providing a tethering compound with two reactive groups: a substrate reactive group and a fluoroalkoxycarbonyl group. The method further involves preparing a substrate-attached tethering group by reacting the substrate reactive group of the tethering compound with a complementary functional group on the surface of a substrate. The substrate-attached tethering group has a fluoroalkoxycarbonyl group that can be reacted with an amino-containing material to form an immobilization group that connects the amino-containing material to the substrate.
    Type: Grant
    Filed: September 17, 2007
    Date of Patent: December 9, 2014
    Assignee: 3M Innovative Properties Company
    Inventors: Karl E. Benson, Charles M. Leir, George G. I. Moore, Rahul R. Shah
  • Patent number: 8889835
    Abstract: The present invention concerns methods and compositions for delivery of therapeutic agents to target cells, tissues or organisms. In preferred embodiments, the therapeutic agents are delivered in the form of therapeutic-loaded polymers that may comprise many copies of one or more therapeutic agents. In more preferred embodiments, the polymer may be conjugated to a peptide moiety that contains one or more haptens, such as HSG. The agent-polymer-peptide complex may be delivered to target cells by, for example, a pre-targeting technique utilizing bispecific or multispecific antibodies or fragments, having at least one binding arm that recognizes the hapten and at least a second binding arm that binds specifically to a disease or pathogen associated antigen, such as a tumor associated antigen. Methods for synthesizing and using such therapeutic-loaded polymers and their conjugates are provided.
    Type: Grant
    Filed: November 7, 2012
    Date of Patent: November 18, 2014
    Assignee: Immunomedics, Inc.
    Inventors: Serengulam V. Govindan, Sung-Ju Moon, David M. Goldenberg, Chien-Hsing Chang
  • Patent number: 8839961
    Abstract: An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.
    Type: Grant
    Filed: November 27, 2006
    Date of Patent: September 23, 2014
    Assignee: FUJIFILM Corporation
    Inventors: Taisei Nishimi, Toshihide Ezoe, Koji Kuruma, Masayuki Kawakami
  • Patent number: 8790882
    Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
    Type: Grant
    Filed: July 3, 2013
    Date of Patent: July 29, 2014
    Assignee: Board of Regents, The University of Texas System
    Inventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
  • Patent number: 8765488
    Abstract: Sensing compositions, sensing element, sensing systems and sensing devices for the detection and/or quantitation of one or more analytes, Compositions comprising carbon nanotubes in which the carbon nanotubes retain their ability to luminesce and in which that luminescence is rendered selectively sensitive to the presence of an analyte. Compositions comprising individually dispersed carbon nanotubes, which are electronically isolated from other carbon nanotubes, yet which are associated with chemical selective species, such as polymers, particularly biological polymers, for example proteins, which can interact selectively with, or more specifically selectivity bind to, an analyte of interest. Chemically selective species bind, preferably non-covalently, to the carbon nanotube and function to provide for analyte selectivity. Chemically selective species include polymers to which one or more chemically selective groups are covalently attached.
    Type: Grant
    Filed: July 22, 2005
    Date of Patent: July 1, 2014
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Michael S. Strano, Seunghyun Baik, Paul Barone
  • Patent number: 8647889
    Abstract: The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.
    Type: Grant
    Filed: November 14, 2012
    Date of Patent: February 11, 2014
    Assignee: Access Medical Systems, Ltd.
    Inventors: Robert F. Zuk, Hong Tan
  • Patent number: 8591904
    Abstract: A conjugate of a modified randomly branched asymmetric polymer without a core and a member of a binding pair is described. The modified randomly branched asymmetric polymer can contain chain branches, terminal branches or both. The modified randomly branched asymmetric polymer can contain random asymmetric branches or random asymmetric junctions. The binding pair can be an antibody, antigen or a ligand. The conjugate includes a drug.
    Type: Grant
    Filed: July 11, 2010
    Date of Patent: November 26, 2013
    Assignee: ANP Technologies, Inc.
    Inventors: Ray Yin, Dujie Qin, Jing Pan
  • Patent number: 8574858
    Abstract: The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte.
    Type: Grant
    Filed: October 2, 2012
    Date of Patent: November 5, 2013
    Assignee: Abbott Laboratories
    Inventors: Maciej Adamczyk, Roy Jeffrey Brashear, Phillip G. Mattingly
  • Patent number: 8568993
    Abstract: A diagnostic method for determining the absence or presence of a disease is provided. The method includes assaying the amount and/or types of glycopeptides in a sample from a subject, and comparing these to the amount and types of reference glycopeptides. The method may include the use of a stable isotope label, affinity selection, immunoaffinity chromatography, and glycoproteomics techniques, to identify and quantify changes in glycosylated peptides or glycosylated proteins associated with cancers such as malignant lymphoma or breast cancer, to monitor patient's response to therapy, and to monitor disease recurrence.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: October 29, 2013
    Assignee: Purdue Research Foundation
    Inventors: Fred E. Regnier, Stephen B. Hooser, Christina R. Wilson, Wonryeon Cho
  • Patent number: 8569078
    Abstract: The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.
    Type: Grant
    Filed: May 3, 2010
    Date of Patent: October 29, 2013
    Assignee: The General Hospital Corporation
    Inventors: Lee Josephson, Ralph Weissleder, J. Manuel Perez
  • Patent number: 8492139
    Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: July 23, 2013
    Assignee: Access Medical Systems, Ltd.
    Inventors: Hong Tan, Robert F. Zuk, Yushan Tan, Erhua Cao, Min Xia, Jun Chen
  • Patent number: 8455265
    Abstract: A surface grafted conjugated polyelectrolyte (CPE) is formed by coupling a CPE by a coupling moiety to the surface of a substrate. The substrate can be of any shape and size, and for many uses of the surface grafted CPE, it is advantageous that the substrate is a nanoparticle or microparticle. Surface grafted CPEs are presented that use silica particles as the substrate, where a modified silane coupling agent connects the surface to the CPE by a series of covalent bonds. Two methods of preparing the surface grafted CPEs are presented. One method involves the inclusion of the surface being modified by the coupling agent and condensed with monomers that form the CPE in a grafted state to the substrate. A second method involves the formation of a CPE with terminal groups that are complimentary to functionality that has been placed on the surface of the substrate by reaction with a coupling agent. The surface grafted CPEs are also described for use as biosensors and biocides.
    Type: Grant
    Filed: February 29, 2008
    Date of Patent: June 4, 2013
    Assignee: STC.UNM
    Inventors: David G. Whitten, Sireesha Chemburu, Thomas Corbitt, Linnea Ista, Gabriel Lopez, Kirk S. Schanze, Motokatsu Ogawa, Eunkyung Ji
  • Patent number: 8415172
    Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.
    Type: Grant
    Filed: October 16, 2012
    Date of Patent: April 9, 2013
    Assignee: Access Medical Systems, Ltd.
    Inventor: Robert F. Zuk
  • Patent number: 8415170
    Abstract: The invention provides a method for continuously detecting glucose concentration in a sample, including: (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose; (c) mixing a sample and the carbohydrate binding protein solution to form a mixture; (d) contacting the mixture with the biosensor; (e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and (f) refreshing the surface of the biosensor with a high concentration glucose solution.
    Type: Grant
    Filed: March 4, 2010
    Date of Patent: April 9, 2013
    Assignee: Industrial Technology Research Institute
    Inventors: Kun-Feng Lee, Chien-An Chen, Hong-Wen Chang, Yuh-Jiuan Lin
  • Patent number: 8415173
    Abstract: A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed.
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: April 9, 2013
    Assignee: Crystal Bioscience Inc.
    Inventor: William Don Harriman
  • Patent number: 8415163
    Abstract: Disclosed is a method of diagnosing renal dysfunction in a cat by removing cauxin from cat urine and detecting a protein in the cat urine sample from which cauxin has been removed. Cauxin is removed from cat urine by bringing the cat urine into contact with a lectin or with an anti-cauxin antibody that specifically binds to cauxin. Cauxin may be removed using a column filled with a carrier that is bound to the lectin or to the anti-cauxin antibody. The lectin may be Lens culinaris lectin. Detection of the cat urinary protein from which cauxin has been removed is performed with a urine test strip.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: April 9, 2013
    Assignees: Incorporated National University Iwate University, Nippon Zenyaku Kogyo Co., Ltd.
    Inventors: Tetsuro Yamashita, Yasuyuki Suzuta
  • Patent number: 8404495
    Abstract: In one aspect, the present invention is a system, a device and a method for sensing the concentration of an analyte in a fluid or matrix. The analyte may be glucose or any other chemical of interest. The fluid or matrix may be, for example, the fluid in a bioreactor, a food or agricultural product, any fluid or matrix in the body of an animal, or any other fluid or matrix whose concentration of an analyte is under investigation. In one embodiment, the analyte sensing device includes a housing having an interior space. Contained within the housing and in the interior space is one or more analyte sensing component(s). The analyte sensing component, in one embodiment, includes one or more radiation converting element(s), for example, converting chromophores. The radiation converting element(s) are capable of converting or modifying radiation of one or more wavelengths into radiation of one or more different wavelengths.
    Type: Grant
    Filed: January 22, 2007
    Date of Patent: March 26, 2013
    Assignee: BioTex, Inc.
    Inventors: Ralph Ballerstadt, Roger McNichols, Ashok Gowda
  • Patent number: 8394647
    Abstract: Methods and reagents are disclosed for reducing an amount of non-covalently bound polysaccharide on a support. The method comprises contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: March 12, 2013
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Bhalchandra Lele, Pratap Singh, Asoke Chatterjee
  • Patent number: 8372660
    Abstract: This invention discloses an analyzing method for detecting a specific analyte in a fluid sample. The method comprises the following steps. First, a substrate is provided. The substrate has a channel provided concavely on an upper surface thereof. The channel comprises a first area, a second area and a third area, and these three areas are connected sequentially. Each of the second and the third areas comprises a nitrocellulose layers containing a reaction material and formed at the bottom thereof. The nitrocellulose layer of the third area can absorb a fixed volume of the fluid sample. Second, the fluid sample is applied to the first area and delivered by the second area and then to the third area. Finally, the reaction material reacts with the specific analyte in the fluid sample to produce a signal for detection.
    Type: Grant
    Filed: February 6, 2009
    Date of Patent: February 12, 2013
    Assignee: Actherm Inc
    Inventors: Wen-Pin Hsieh, Yi-Jen Wu
  • Patent number: 8361810
    Abstract: Methods are disclosed for producing a bioweapon-sensitive fibrous-network product, wherein the subject products exhibit a color change in response to exposure to a biological agent (or portion thereof) as used in a biological weapon. Also disclosed are fibrous-network products that contain units of biopolymeric material that impart a color change to the products in response to exposure to a biological agent (or portion thereof) as used in a biological weapon.
    Type: Grant
    Filed: April 2, 2009
    Date of Patent: January 29, 2013
    Inventors: Dennis Farwell, Keith Baumann
  • Patent number: 8349618
    Abstract: The present invention relates to analytical methods, platforms, and devices for the rapid and efficient immunochromatic determination of one or more components in fluid samples.
    Type: Grant
    Filed: November 23, 2010
    Date of Patent: January 8, 2013
    Assignee: ZBX Corporation
    Inventor: Qinwei Shi
  • Patent number: 8338140
    Abstract: The present invention concerns methods and compositions for delivery of therapeutic agents to target cells, tissues or organisms. In preferred embodiments, the therapeutic agents are delivered in the form of therapeutic-loaded polymers that may comprise many copies of one or more therapeutic agents. In more preferred embodiments, the polymer may be conjugated to a peptide moiety that contains one or more haptens, such as HSG. The agent-polymer-peptide complex may be delivered to target cells by, for example, a pre-targeting technique utilizing bispecific or multispecific antibodies or fragments, having at least one binding arm that recognizes the hapten and at least a second binding arm that binds specifically to a disease or pathogen associated antigen, such as a tumor associated antigen. Methods for synthesizing and using such therapeutic-loaded polymers and their conjugates are provided.
    Type: Grant
    Filed: September 27, 2011
    Date of Patent: December 25, 2012
    Assignee: Immunomedics, Inc.
    Inventors: Serengulam V. Govindan, Sung-Ju Moon, David M. Goldenberg, Chien-Hsing Chang
  • Patent number: 8309369
    Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.
    Type: Grant
    Filed: September 1, 2011
    Date of Patent: November 13, 2012
    Assignee: Access Medical Systems, Ltd.
    Inventors: Hong Tan, Robert F. Zuk, Yushan Tan, Erhua Cao, Min Xia, Jun Chen
  • Patent number: 8268639
    Abstract: A method for diagnosing a kidney disease in a cat by detecting a cat urinary protein without being affected by cauxin, and a diagnostic agent therefor are provided. A method of detecting a urinary protein derived from renal dysfunction in a cat according to claim 1, wherein cauxin is removed from cat urine by bringing the cat urine into contact with a lectin or an anti-cauxin antibody that specifically binds to cauxin.
    Type: Grant
    Filed: July 20, 2007
    Date of Patent: September 18, 2012
    Assignees: Incorporated National University Iwate University, Nippon Zenyaku Kogyo Co., Ltd.
    Inventors: Tetsuro Yamashita, Yasuyuki Suzuta
  • Patent number: 8241574
    Abstract: An ophthalmic device which comprises a holographic element comprising a medium comprising a phenylboronic acid group and, disposed therein, a hologram, wherein an optical characteristic of the element changes as a result of a variation of a physical property of the medium, and wherein the variation arises as a result of interaction between the medium and an analyte present in an ocular fluid.
    Type: Grant
    Filed: July 28, 2011
    Date of Patent: August 14, 2012
    Assignee: Smart Holograms Limited
    Inventors: Barry Burles, Roger Bradley Millington, Christopher Robin Lowe, Satyamoorthy Kabilan, Jeffrey Blyth
  • Patent number: 8236575
    Abstract: A first reactant, which is provided with a reaction site for specific binding with an analyte, and a fluorescent label site, and a second reactant, which is provided with a reaction site for specific binding with the analyte, and a fluorescence recognition site for recognizing fluorescence produced by the fluorescent label site of the first reactant, are respectively fixed onto a support such that the first reactant and the second reactant have a positional relationship adapted for the binding with the analyte.
    Type: Grant
    Filed: November 21, 2008
    Date of Patent: August 7, 2012
    Assignee: FUJIFILM Corporation
    Inventors: Koichi Minami, Hirohiko Tsuzuki
  • Publication number: 20120196385
    Abstract: An assay method with use of a sensor chip which includes a metal member, a self-assembled monolayer (SAM), and ligands on a support, and is configured to be used for a fluorescence measuring apparatus with utilization of a surface plasmon-field enhanced Fluorescence Spectrometry, including the steps of: forming a hydrophilic high molecule layer on the self-assembled monolayer in the sensor chip; immobilizing the ligands at least one of in the hydrophilic high molecule layer and on the surface of the hydrophilic high molecule layer; and bringing a moisturizer in contact with the hydrophilic high molecule layer.
    Type: Application
    Filed: January 19, 2012
    Publication date: August 2, 2012
    Applicant: KONICA MINOLTA HOLDINGS, INC.
    Inventors: Noriaki YAMAMOTO, Takatoshi KAYA
  • Patent number: 8232068
    Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
    Type: Grant
    Filed: January 12, 2012
    Date of Patent: July 31, 2012
    Assignee: Board of Regents, The University of Texas System
    Inventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
  • Publication number: 20120171782
    Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
    Type: Application
    Filed: March 15, 2012
    Publication date: July 5, 2012
    Inventors: Randall W. Nelson, Peter Williams, Jennifer Reeve Krone
  • Patent number: 8183002
    Abstract: The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte.
    Type: Grant
    Filed: December 3, 2009
    Date of Patent: May 22, 2012
    Assignee: Abbott Laboratories
    Inventors: Maciej Adamczyk, Jeffrey R. Brashear, Phillip G. Mattingly
  • Patent number: 8163563
    Abstract: The invention provides devices, test kits and methods for removing target agents from a sample. The device contains one or more porous matrices having pore sizes larger than 10 ?m, and a plurality of particles impregnated therein. The target agents attach the device and are removed from the sample.
    Type: Grant
    Filed: June 9, 2005
    Date of Patent: April 24, 2012
    Assignee: Pathogen Removal and Diagnostic Technologies, Inc.
    Inventor: Ruben G. Carbonell
  • Patent number: 8133691
    Abstract: The invention relates to a kit comprising MHC Class I and Class II HLA-coated beads containing specific antigenic peptides for binding to antigen-specific T cells and the appropriate negative control peptides. Also provided are methods for making the coated beads and methods for use. The application of these beads go to the stimulation of peripheral blood cell populations and in vitro-stimulated culture for the elicitation of functional activities such as cell activation and signaling, cytokine secretion, proliferation and cytotoxicity activity.
    Type: Grant
    Filed: July 5, 2006
    Date of Patent: March 13, 2012
    Assignee: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.
    Inventors: Sathibalan Ponniah, George E. Peoples, Catherine E. Storrer, Michael Flora
  • Patent number: 8133743
    Abstract: Phenobarbital derivatives synthesized out of the alkyl chain at the 5-position, particularly with hydrophilic properties, and carrying an active ester at the end, allow formation of aminodextran conjugates that give curves in the desired range of the assay in the ONLINE TDM microparticle assay format when matched against the Roche FPIA antibody specific for phenobarbital (“an antibody specific for phenobarbital”).
    Type: Grant
    Filed: September 23, 2008
    Date of Patent: March 13, 2012
    Assignee: Roche Diagnostics Operations, Inc.
    Inventor: Raymond Hui
  • Patent number: 8124359
    Abstract: A method for reducing non-specific binding in an assay is provided herein. The method includes (a) providing a reaction mixture, which includes or is suspected to include a first component and a second component capable of binding to each other in a specific binding reaction, and (b) adding non-physiological amounts of at least one additive to the reaction mixture before, during or after binding in a sufficient amount to reduce non-specific binding in the reaction mixture. The method further includes (c) monitoring or measuring the presence and/or concentration of at least one of the first and second components after step (b).
    Type: Grant
    Filed: March 16, 2007
    Date of Patent: February 28, 2012
    Assignee: Aokin AG
    Inventors: Stuart Levison, Paul Nix, Derek Levison
  • Patent number: 8114615
    Abstract: The invention provides an improved method for identifying and interpreting tissue specimens and/or cells derived from tissue specimens. A panel of cell-based reagents provides a number of readouts of cellular states or biomarkers that together define a profile of a diversity of cellular states or biomarkers in a tissue specimen representing the “systems” nature of biology. This cellular profile is interpreted using informatics tools, to identify similarities between specimens, in vivo medical conditions, and suggest options for treating medical conditions.
    Type: Grant
    Filed: May 17, 2007
    Date of Patent: February 14, 2012
    Assignee: Cernostics, Inc.
    Inventors: Albert H. Gough, Kenneth A. Giuliano, D. Lansing Taylor
  • Patent number: 8114618
    Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
    Type: Grant
    Filed: April 5, 2011
    Date of Patent: February 14, 2012
    Assignee: Board of Regents of the University of Texas System
    Inventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
  • Patent number: 8114661
    Abstract: The present invention is directed to the detection of target analytes using electronic techniques, particularly AC techniques.
    Type: Grant
    Filed: May 4, 2010
    Date of Patent: February 14, 2012
    Assignee: Osmetech Technology, Inc.
    Inventors: Stephen D. O'Connor, Jon Faiz Kayyem, Thomas J. Meade
  • Patent number: 8114616
    Abstract: The invention provides an ELISA assay for the determination of serum mast cell ?-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of ?-tryptase, histamine and/or prostaglandin E2.
    Type: Grant
    Filed: August 24, 2010
    Date of Patent: February 14, 2012
    Assignee: Prometheus Laboratories Inc.
    Inventors: Hua Gong, Shui Long Wang, Sharat Singh
  • Patent number: 8110366
    Abstract: The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.
    Type: Grant
    Filed: July 7, 2006
    Date of Patent: February 7, 2012
    Assignees: Suomen Punainen Risti, Veripalvelu, Glykos Finland Oy
    Inventors: Jarmo Laine, Taina Jaatinen, Heidi Anderson, Johanna Nystedt, Sarl Tiitinen, Anita Laitinen, Ulla Impola, Tero Satomaa, Jari Natunen, Annamari Heiskanen, Maria Blomqvist, Anne Olonen
  • Patent number: 8110365
    Abstract: Renal injury and inflammation is diagnosed by detecting an elevation in GSK3b level or activity. Inflammation of bodily tissues such as renal tissue is inhibited by administration of GSK3b inhibitory compositions.
    Type: Grant
    Filed: March 29, 2007
    Date of Patent: February 7, 2012
    Assignee: Rhode Island Hospital
    Inventors: Lance Dworkin, Rujun Gong
  • Patent number: 8105788
    Abstract: Methods, systems, kits and assays for diagnosing, monitoring or screening for mucopolysaccharidosis (MPS) and methods and systems for assaying test compounds for therapeutic activity are described, whereby MPS marker protein levels are assayed as an indication of MPS.
    Type: Grant
    Filed: December 8, 2006
    Date of Patent: January 31, 2012
    Assignee: The University of British Columbia
    Inventors: Lorne A. Clarke, Derrick Raymond Randall, Graham Sinclair
  • Patent number: 8105787
    Abstract: The present invention relates to a method of isolating fragments of nucleic acid according to the density of CpG dinucleotides and subsequent procedures for producing a library and/or an array or microarray of these fragments, as well as uses thereof.
    Type: Grant
    Filed: October 31, 2005
    Date of Patent: January 31, 2012
    Assignee: The University Court of the University of Edinburgh
    Inventors: Adrian Peter Bird, Robert Scott Illingworth, Helle Faerk Jorgensen
  • Patent number: 8097427
    Abstract: The invention relates to a method of using high mass matrix assisted laser desorption-ionization (MALDI) mass spectrometry for the qualitative and quantitative analysis of the effect of drug candidates on protein complexes such as protein-protein interactions in purified samples or complex biological matrices, as well as to the use of this method for lead compound optimization, drug characterization, drug manufacturing processes, and drug quality control processes, including automated high throughput applications.
    Type: Grant
    Filed: March 14, 2008
    Date of Patent: January 17, 2012
    Assignee: CovalX AG
    Inventors: Alexis Txomin Nazabal, Ryan Jay Wenzel
  • Patent number: 8093005
    Abstract: A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.
    Type: Grant
    Filed: October 19, 2009
    Date of Patent: January 10, 2012
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Tanja Jarhede, Per Kjellin, Anita Larsson, Hans Sjobom