Cellulose Or Derivative Patents (Class 436/530)
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Patent number: 6159747Abstract: The invention concerns an analytical test element containing an absorbent material with a sample application and detection zone and a blister filled with liquid on a support, wherein the support has a notch within the support surface such that a spike forms there when the support is bent with which the blister can be opened so that liquid escapes and comes into contact with the absorbent material as well as a process for the production of such an analytical test element and a method for determining an analyte by means of such an analytical test element.Type: GrantFiled: February 23, 1998Date of Patent: December 12, 2000Assignee: Roche Diagnostics GmbHInventors: Herbert Harttig, Christian Klein
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Patent number: 6150123Abstract: The present invention relates to a method for biotinylating a desired subset of a larger population of proteins, wherein the subset of proteins is first selectively bound to a solid phase, thereby separating it from undesired proteins of the population, and then, still bound to solid phase, it is biotinylated. Unreacted biotin may then be washed from the solid phase, and the biotinylated protein may be uncoupled from the solid phase.Type: GrantFiled: September 3, 1998Date of Patent: November 21, 2000Assignee: Centre Integre de Recherches Biocliniques sur le Sida (CIRBS)Inventor: Antonio Cosma
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Patent number: 6143576Abstract: The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include, flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.Type: GrantFiled: March 3, 1997Date of Patent: November 7, 2000Assignee: Biosite Diagnostics, Inc.Inventor: Kenneth F. Buechler
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Patent number: 6140136Abstract: The present invention provides an analytical test device for conducting assays of biological fluids. Methods for carrying out the assays with the disclosed analytical test device are also provided.Type: GrantFiled: September 18, 1998Date of Patent: October 31, 2000Assignee: Syntron BioResearch, Inc.Inventor: Jin Po Lee
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Patent number: 6129916Abstract: Disclosed are conjugates including a polymer backbone or microbead and binding molecules, such as Fv, Fab, or F(ab').sub.2 fragments of monoclonal antibodies or whole antibodies that are bound through their Fc carbohydrate moieties or have their Fc portion modified so that they cannot effect ADCC or complement-mediated cytolysis, and that are specific for a T cell surface antigen, such as CD3, TCR, CD4, CD8, or CD28 on T cells. The polymer or microbead is preferably made of cross-linked dextran, ficoll, latex, or agarose. The microbeads are preferably of 1 to 10 .mu.m in size, so that they can be suspended in in vivo fluids. These conjugates can be used to induce proliferation of T cells and immune stimulation, and to increase the antibody response against an administered antigen.Type: GrantFiled: November 25, 1992Date of Patent: October 10, 2000Assignee: Tanox, Inc.Inventor: Tse Wen Chang
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Patent number: 6130100Abstract: A process of manufacturing test strips of the type comprising a length of porous carrier material capable of acting as a liquid flow path for a sample liquid and having at least zone downstream from a first end of the strip which zone contains an immobilized specific binding agent to act as a capture means during an assay to reveal the presence of an analyte in applied sample liquid, and in which process the specific binding agent is deposited onto a sheet of the porous carrier material which is then blocked and subdivided into a plurality of individual identical test strips, wherein blocking of the porous carrier material is achieved by applying a solution of blocking agent to the sheet upstream from the zone in an amount sufficient to ensure that the solution permeates downstream to beyond the zone.Type: GrantFiled: September 23, 1997Date of Patent: October 10, 2000Assignee: Unilever Patent Holdings B.V.Inventors: Ian Jobling, David A. Percival, Michael E. Prior
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Patent number: 6127136Abstract: A method of detecting dioxin-like compounds consisting essentially of the group of compounds: polychlorinated dibenzodioxins, polychlorinated dibenzofurans, polychlorinated biphenyls, and structural analogues thereof which exhibit biological activity characteristic of polychlorinated dibenzodioxins, polychlorinated dibenzofurans, polychlorinated biphenyls. The assay includes: 1) an inactive Ah receptor in a form capable of binding to the dioxin-like compounds and being transformed to an active form that forms a complex with ARNT and binds a dioxin responsive element, and 2) a quantity of ARNT sufficient to optimize Ah receptor transformation. A test sample is contacted with the assay under conditions effective to bind the dioxin-like compounds to the Ah receptor and allow transformation of the Ah receptor to an active form that forms a complex with ARNT. The presence of the complex containing the transformed Ah receptor and the ARNT is detected.Type: GrantFiled: February 14, 1996Date of Patent: October 3, 2000Assignee: Paracelsian, Inc.Inventor: Geoffrey D. Wheelock
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Patent number: 6127190Abstract: A technique is disclosed for generating nonrandom combinatorial libraries on solid phase supports in which each of a set of predetermined species of test compounds is present on a predetermined number of solid phase supports, preferably on only one, and each solid phase support has only a single species of test compound. Each of the predetermined species of test compounds is prepared with absolute certainty because the technique does not employ any random division of the solid phase supports. Rather, the method is based on the stepwise division of a continuous solid phase support matrix prior to each synthetic step in which more than one type of subunit is added. Non-limiting examples of matrices of the solid phase supports include polypropylene membranes, polytetrafluoropropylene membranes and cotton thread. The combinatorial libraries made by the technique are also disclosed.Type: GrantFiled: November 17, 1997Date of Patent: October 3, 2000Assignee: Selectide CorporationInventor: Michal Lebl
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Patent number: 6127130Abstract: A method of determining the concentration of a sample antigen in the presence of an interferant by(1) running two immunoassays on the sample: one assay where the interferant influences the binding of both the sample antigen and a labeled antigen and a second assay where the interferant influences the binding of the sample antigen but not the labeled antigen;(2) obtaining a plot of the possible sample antigen concentrations versus the possible interferant concentrations corresponding to the readout for the sample for each of the two immunoassays; and(3) determining the sample antigen concentration and the interferant concentration which correspond to the point that appears in both the immunoassays plots.Type: GrantFiled: March 13, 1998Date of Patent: October 3, 2000Assignee: The United States of America as represented by the Secretary of the NavyInventor: Robert A. Brizzolara
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Patent number: 6121056Abstract: Methods, compositions and articles of manufacture are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. At least two dendrimer-reagent preparations with different analyte specificities may be immobilized on a solid phase. Alternatively, at least one dendrimer-reagent preparation having multiple specificities may be immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.Type: GrantFiled: April 12, 1994Date of Patent: September 19, 2000Assignee: Dade Behring Inc.Inventors: Fred Moll, III, Charles Ferzli, Spencer H. Lin, Pratap Singh
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Patent number: 6093546Abstract: The invention relates to a process for making a test element, such as an analytical test element. Compositions of matter, such as analyte specific receptors, are treated to carry a charge. These are then applied to test carriers treated to carry a charge opposite that of the composition of matter. Interaction of charge fixes the composition of matter to the test element.Type: GrantFiled: September 3, 1992Date of Patent: July 25, 2000Assignee: Roche DiagnosticsInventors: David J. Ledden, David R. Moorman, Michael J. Kinch, Harvey B. Buck
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Patent number: 6087185Abstract: The prevent invention relates to an integrated package-holder assay devices for detecting the presence of analyte in a sample. The device serves the dual roles of supporting and protecting an immunochromatographic assay. The device is compatible with any immunochromatographic assay format. The assay can be performed in a single apparatus for use in a laboratory or a field setting. In a specific example, the assay device is a nylon membrane formatted for an immunochromatographic assay for cotinine sealed between transparent adhesive tape and a stiff plastic strip. White tape placed over the plastic strip defined a window for observing the assay results.Type: GrantFiled: November 17, 1997Date of Patent: July 11, 2000Assignee: Serex, Inc.Inventors: F. Victor Lee-Own, Judith Fitzpatrick
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Patent number: 6083758Abstract: Methods for identifying polypeptides which coordinate to select metals, chemosensors comprising polypeptides which coordinate to select metals and methods for selectively detecting the presence of metals using these chemosensors are disclosed.Type: GrantFiled: April 9, 1997Date of Patent: July 4, 2000Assignee: California Institute of TechnologyInventors: Barbara Imperiali, Grant K. Walkup
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Patent number: 6078782Abstract: The invention relates to methods of performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. Particles are employed in the method, which are then collected in a zone at which electrochemiluminescence can be induced, wherein the amount of induced electrochemiluminescence is related to the amount of analyte in the sample.Type: GrantFiled: June 7, 1995Date of Patent: June 20, 2000Assignee: IGEN International Inc.Inventors: John K. Leland, Haresh P. Shah, John H. Kenten, Jack E. Goodman, George E. Lowke, Yuzaburo Namba, Gary F. Blackburn, Richard J. Massey
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Patent number: 6048715Abstract: A two-phase partition system is provided for affinity separation of a composition containing a polysaccharide binding peptide from a mixture such as a fermentation broth. The peptide may be from an enzyme and lacking in polysaccharidase activity such as the binding domain of cellulase that binds to cellulose. The system contains a phase-forming oligosaccharide polymer such as a cellulose derivative to which the peptide binds with a Ka of 10.sup.3 M to 10.sup.7 M, and a phase inducing agent such as a polyethylene glycol polymer, or a salt present at sufficiently high concentration to induce phase separation. If the oligosaccharide polymer is thermoseparating, phase separation can be induced by heating. Using the system involves contacting a composition containing the peptide such as a fusion protein with the system, partitioning the composition into a phase containing the oligosaccharide polymer by binding to the polymer and recovering the polymer containing the bound composition.Type: GrantFiled: July 24, 1996Date of Patent: April 11, 2000Assignee: Univ. of British ColumbiaInventors: Charles A. Haynes, Peter Tomme, Douglas G. Kilburn
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Patent number: 6043087Abstract: The invention provides a monospecific antibody that is specifically reactive with enzymatically mediated degradation products of fibrin(ogen) (i.e., fibrin, fibrinogen, and related substances). The monospecific antibody of the invention is specifically reactive with an epitope defined by an amino acid sequence SEQ ID NO:1. The invention further provides compositions containing a monospecific antibody, optionally detectably labeled, for the performance of fibrinolytic or thrombolytic analyses. The invention further provides continuous cell lines (hybridomas) that produce monospecific antibodies as described.Type: GrantFiled: July 25, 1997Date of Patent: March 28, 2000Assignee: The New York Blood CenterInventors: Alessandra Bini, Bohdan J. Kudryk
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Patent number: 6033918Abstract: Disclosed is an improvement to the technique for immobilizing an analyte onto a solid surface for use in a competitive immunoassay. The improvement involves immobilizing the analyte on the solid surface by reacting the analyte or derivative thereof with polyethylene glycol which has been functionalized with a group which is reactive with the analyte contacting the resulting conjugate with the solid surface to thereby immobilize the analyte.Type: GrantFiled: November 10, 1997Date of Patent: March 7, 2000Assignee: Bayer CorporationInventors: Robert P. Hatch, Meitak Teresa Yip
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Patent number: 6030845Abstract: An immunoassay method in which blood can be measured even without pretreatment by a centrifuge etc. In the present invention, antibodies or antigens in a sample are subjected to agglutination reaction with insoluble carriers onto which antigens or antibodies specifically reacting with the antibodies or antigens in the sample have been imrobilized and the resulting agglutination mixture is determined for the change in its absorbance or in its scattered light by irradiation with light, wherein said sample is whole blood and the whole blood is forcibly lysed.Type: GrantFiled: July 28, 1997Date of Patent: February 29, 2000Assignee: Horiba Ltd.Inventors: Yasuo Yamao, Narihiro Oku
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Patent number: 6027943Abstract: An immunochemical assay device comprising a base member, an array disposed on the base member, and at least one assay indicia zone. The array comprises (i) a reservoir pad to receive sample liquid, (ii) a wicking membrane, and (iii) at least one filter zone interposed between the wicking membrane and the reservoir pad. The filter zone being operable to permit passage of any specific immunocomplex to the wicking membrane while impeding passage of larger components.Type: GrantFiled: November 14, 1997Date of Patent: February 22, 2000Assignee: Princeton Biomeditech CorporationInventors: Jemo Kang, Byungwoo Youn, Young Ho Oh
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Patent number: 6027877Abstract: A method for detecting mutations, such as a single base change or an addition or deletion of about one to four base pairs, is based on the use of an immobilized DNA mismatch-binding protein, such as MutS, which binds to a nucleic acid hybrid having a single base mismatch or unpaired base or bases, thereby allowing the detection of mutations involving as little as one base change in a nucleotide sequence. Such a method is useful for diagnosing a variety of important disease states or susceptibilities, detecting the presence of a mutated oncogene and for isolating or removing by affinity chromatography duplex DNA molecules containing mismatches such as error-containing molecules in PCR-amplified DNA samples. Also provided are kits useful for practicing the methods of the present invention.Type: GrantFiled: November 4, 1993Date of Patent: February 22, 2000Assignee: Gene Check, Inc.Inventor: Robert E. Wagner, Jr.
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Patent number: 6017768Abstract: Combinatorial libraries are disclosed which are represented by Formula I: ##STR1## wherein: ##STR2## is a solid support; T'--L-- is an identifier residue; and --L'--II' is a ligand/linker residue. These libraries contain dihydrobenzopyrans of the formula ##STR3## which interact (i.e., as agonists or antagonists) with .alpha. adrenergic receptors, dopamine receptors, .sigma.-opiate receptors, and K.sup.+ channels and are inhibitors of carbonic anhydrase isozymes. They are useful in the treatment of ocular diseases such as glaucoma.Type: GrantFiled: October 18, 1996Date of Patent: January 25, 2000Assignee: Pharmacopeia, Inc.Inventors: John J. Baldwin, John C. Reader, Lawrence W. Dillard, Ge Li, Wenguang Zeng
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Patent number: 6015681Abstract: An assay method and kit for detecting specific oral cariogenic bacteria, ., mutans streptococci, Lactobacillus sp. and Actinomyces sp., separately or in combination, comprising gathering a sample suspected of containing cariogenic bacteria; treating the sample with a stripping buffer to remove host antibodies from bacteria present in the sample; retaining the treated bacteria on a blocked solid phase substrate; reacting the retained bacteria with a primary antibody specific for the desired cariogenic bacteria; reacting the primary antibody with a conjugated label producing a detectable signal; and detecting the signal whereby the presence of the desired cariogenic bacteria is determined in the sample. The device for conducting these assays is a frame or support which holds a solid substrate capable of retaining the bacteria of interest while permitting drainage of other materials or fluids away from the retained bacteria.Type: GrantFiled: December 12, 1996Date of Patent: January 18, 2000Assignee: The United States of America as represented by the Secretary of the NavyInventors: Stephen Alden Ralls, Lloyd Grant Simonson
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Patent number: 6016345Abstract: An anonymous testing system for taking a sample of body fluid to be tested, the sample is acquired in private and sent for analyzation to obtain results. The system comprises a test kit for creating a sample of body fluid, a personal code for anonymously identifying the sample and the person, and an electronic file telephonically created and accessed by the person taking the test and identified by the personal code. The electronic file contains the test result determined by analyzation. There is also a method of anonymously testing of a person's body fluid in private and the results to be anonymously received by the person without having to reveal his or her identification. The method comprises the steps of procuring a test kit for taking a sample of the body fluid. The test kit has a personal code associated therewith and equipment to take the sample. The person uses the equipment to obtain the sample and sends the sample with the personal code to a testing site.Type: GrantFiled: March 21, 1997Date of Patent: January 18, 2000Assignee: Home Access Health CorporationInventor: Richard A. Quattrocchi
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Patent number: 6014438Abstract: An anonymous testing system for taking a sample of body fluid to be tested, the sample is acquired in private and sent for analyzation to obtain results. The system comprises a test kit for creating a sample of body fluid, a personal code for anonymously identifying the sample and the person, and an electronic file telephonically created and accessed by the person taking the test and identified by the personal code. The electronic file contains the test results determined by analyzation. There is also a method of anonymously testing of a person's body fluid in private and the results to be anonymously received by the person without having to reveal his or her identification. The method comprises the steps of procuring a test kit for taking a sample of the body fluid. The test kit has a personal code associated therewith and equipment to take the sample. The person uses the equipment to obtain the sample and sends the sample with the personal code to a testing site.Type: GrantFiled: June 17, 1997Date of Patent: January 11, 2000Assignee: Home Access Health CorporationInventor: Richard A. Quattrocchi
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Patent number: 6013460Abstract: Modified Western blot membranes and methods of using same are provided which allow confirmation of Lyme disease and screening for at least one additional tick-borne disease. The membranes and methods of the present invention may thus be used to screen for the presence of tick-borne diseases which may be transferred along with Lyme disease. A Western blot assay may also be employed to confirm the presence of such additional tick-borne disease.Type: GrantFiled: September 12, 1997Date of Patent: January 11, 2000Assignee: Immunetics, IncorporatedInventor: Andrew E. Levin
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Patent number: 6007999Abstract: Disclosed are devices and methods for performing an assay which determines the presence or quantity of an analyte in a fluid sample by detecting binding of the analyte to at least one immobilized analyte capture reagent and washing unbound material from the immobilized analyte capture reagent. The devices and methods an elongated solid phase flow matrix, including capillary channels capable of driving capillary fluid movement, and further including (i) a first region adapted for receipt of the fluid sample, (ii) a second region at which the analyte capture reagent is immobilized, (iii) a third region for application of a wash reagent capable of removing unbound substances from the second region; and (iv) an absorbent reservoir of high volume capacity. The second region is positioned intermediate to the first region and the third region and intermediate to the absorbent reservoir and the third region. The device also includes means to detect analyte bound at the second region.Type: GrantFiled: March 9, 1998Date of Patent: December 28, 1999Assignee: IDEXX Laboratories, Inc.Inventor: Scott M. Clark
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Patent number: 6008056Abstract: A method of assaying a preselected volume of sample on an chromatographic strip includes providing a sample receiving reservoir. This reservoir can receive a preselected volume of sample or a preselected volume of sample and reagent. The sample receiving reservoir has an overflow outlet. A moat is communicated to the overflow outlet of the sample receiving reservoir for receiving the sample or sample and reagent when the sample receiving reservoir is full. The mixed sample or sample and reagent fill the sample receiving reservoir, and discharge the excess sample through the overflow outlet to the moat. The sample receiving reservoir having the preselected volume of sample or sample and reagent is discharged on the chromatographic strip for assay. Provision is made for adding a density adding solute which is neutral to the chromatographic strip, the density adding solute occupying the sample receiving reservoir.Type: GrantFiled: May 28, 1997Date of Patent: December 28, 1999Assignee: Epitope, Inc.Inventor: Thomas Thieme
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Patent number: 5998220Abstract: A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (1) a first opposable component comprising a sample preparation means adapted to receive a sample to be assayed; and (2) a second opposable component comprising a chromatographic medium. The first and second opposable components can be brought into opposition so as to cause the sample preparation means to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analytes, as well as to permit bi-directional chromatography.Type: GrantFiled: February 10, 1994Date of Patent: December 7, 1999Assignee: Beckman Coulter, Inc.Inventor: Howard M. Chandler
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Patent number: 5998221Abstract: The invention is directed to a non-instrumented assay giving quantitative and/or qualitative results. Specifically, the invention is directed to an assaying process wherein a first portion of binder supported on substantially the entire length of a solid is contacted with a sample suspected of containing analyte. The binder is a binder for at least the analyte. The first portion is also contacted with a tracer. The process includes contacting a second binder portion also supported on the solid with tracer. The second portion is juxtaposed to the first portion and has a known amount of analyte thereon. The amount of analyte in the sample is determined by comparing the visibility of tracer in the first and second portions. In a preferred embodiment, a third binder portion supported on the solid juxtaposed to the second portion is contacted with tracer. The third portion has a known amount of analyte thereon. The amount of analyte in the third portion is different from the amount on the second portion.Type: GrantFiled: September 25, 1996Date of Patent: December 7, 1999Assignee: Becton, Dickinson and CompanyInventors: Adrien Paul Malick, Edward Charles McFarland
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Patent number: 5989925Abstract: A monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, Md., under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.Type: GrantFiled: June 27, 1997Date of Patent: November 23, 1999Assignee: Serex, Inc.Inventors: Judith Fitzpatrick, Regina Lenda, Zijian Zeng
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Patent number: 5989924Abstract: The present invention relates to a device for determining an analyte in a fluid sample in an assay. The device comprises a lid having a plurality of elements and a membrane. The elements have openings which allow passage of fluid to the membrane. The membrane is supported by the lid and covers the openings of the elements. The undersurface of the membrane is in contact with a porous wick which contains a first zone which includes binder attached to a particulate label and a second zone containing salt. The wick extends downward from the membrane and is in contact with the fluid sample.Type: GrantFiled: September 30, 1997Date of Patent: November 23, 1999Assignee: Becton, Dickinson and CompanyInventors: Richard T. Root, Stephen J. Lovell
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Patent number: 5985675Abstract: A test device, system and method, the device composed of an elongated, toothbrush-shaped, transparent, plastic housing and a lateral-flow test strip for the detection of an analyte, such as a beta-lactam in milk, in the housing, the housing having an expansion cavity to receive expanded, liquid-contacted, absorbing material in the test strip, and to control lateral flow rate and times in the test strip.Type: GrantFiled: December 31, 1997Date of Patent: November 16, 1999Assignee: Charm Sciences, Inc.Inventors: Stanley E. Charm, Richard Skiffington, Robert J. Markovsky, Eliezer Zomer
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Patent number: 5985581Abstract: The invention provides a method of diagnosing Alzheimer's disease. The method utilizes presenilin-1, whose level is found to be substantially decreased in Alzheimer's patients. Included in the invention are diagnostic kits for Alzheimer's disease and methods of screening for effective therapeutics for the disease. The invention also provides a method of studying the function and regulation of presenilin-1 in brain by the use of primate retinoblastoma cells.Type: GrantFiled: July 17, 1997Date of Patent: November 16, 1999Assignee: The McLean Hospital CorporationInventors: Ralph A. Nixon, Anne M. Cataldo, Benjamin H. Kao, Paul M. Mathews
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Patent number: 5981298Abstract: A device and related method for performing one or more immunoassays to detect the presence of respective analytes in a sample. The device involves the use of a unitary bibulous material providing one or more flow paths having a common origin site and a plurality of respective reagent zones providing the reagents necessary for performing a visual readout, competitive immunoassay for the presence of the respective analyte.Type: GrantFiled: September 30, 1997Date of Patent: November 9, 1999Assignee: Surmodics, Inc.Inventors: Stephen J. Chudzik, Martha J. Hamilton
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Patent number: 5978466Abstract: An anonymous testing system for taking a sample of body fluid to be tested, the sample is acquired in private and sent for analyzation to obtain results. The system comprises a test kit for creating a sample of body fluid, a personal code for anonymously identifying the sample and the person, and an electronic file telephonically created and accessed by the person taking the test and identified by the personal code. The electronic file contains the test results determined by analyzation. There is also a method of anonymously testing of a person's body fluid in private and the results to be anonymously received by the person without having to reveal his or her identification. The method comprises the steps of procuring a test kit for taking a sample of the body fluid. The test kit has a personal code associated therewith and equipment to take the sample. The person uses the equipment to obtain the sample and sends the sample with the personal code to a testing site.Type: GrantFiled: October 2, 1997Date of Patent: November 2, 1999Assignee: Home Access Health CorporationInventor: Richard A. Quattrocchi
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Patent number: 5972717Abstract: A method of detecting heparin-induced antibodies to complete a diagnosis of heparin-induced thrombocytopenia (HITP) is disclosed. This method comprises the first step of attaching a glycosaminoglycan to a solid support, wherein the glycosaminoglycan is attached to the solid support only at the reducing end of the molecule (unidirectionally). Platelet factor 4 is then bound to the glycosaminoglycan forming a complex having an epitope recognizable by antibodies generated in an HITP immune response. Human blood plasma or serum from a patient suspected of having HITP is exposed to the complex and the complex is analyzed to determine if HITP-related antibodies are present. A device and kit used in performing the diagnostic assay are also disclosed.Type: GrantFiled: November 19, 1997Date of Patent: October 26, 1999Assignee: The Blood Center Research Foundation, Inc.Inventors: Richard H. Aster, Gian Visentin
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Patent number: 5968758Abstract: Antibodies having binding affinity for free IGFBP-1, biological compositions including antibodies having binding affinity for free IGFBP-1, kits for detecting free IGFBP-1 using the antibodies, and cell lines for producing the antibodies are provided. Also provided are devices and methods for detecting free IGFBP-1 and a rupture in a fetal membrane based on the presence of amniotic fluid in a vaginal secretion, as indicated by the presence of free IGFBP-1 in the vaginal secretion. The antibodies that are provided may be characterized by their ability to selectively recognize those IGFBP-1 molecules which are free of IGF-1 and IGF-2, i.e., antibodies which have a binding affinity for free IGFBP-1 that is greater than a binding affinity of the antibody to bound IGFBP-1. These antibodies may also be characterized by their competition with IGF-1 and IGF-2 for binding to IGFBP-1.Type: GrantFiled: October 28, 1996Date of Patent: October 19, 1999Assignee: California Research LLCInventors: Boris Fuks, Marina Boltovskaya, Alexander Konstantinov, Svetlana Nazimova, Nelli Starosvetskaya, Alexander Stepanov, Evgeny Zaraisky
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Patent number: 5965458Abstract: A test strip for a rapid immunoassay containing specific immunochemical reagent zones (7, 8, 9) is described. The test strip (10) has a backing sheet (1) and attached thereto a receiving end pad (3) and at a distance therefrom a finishing end pad (5). A test membrane (2) is provided between said pads (3, 5). Said membrane (2) is positioned in parallel relationship to said backing (1) at a distance therefrom so that said backing (1) and said membrane (2) limit between themselves an air gap (4) which is open at its edges. Said gap (4) functions as a sheltered reaction chamber for the immunolgical reaction taking place as a liquid is made to flow through said membrane (2). The test strip is simple to manufacture by lamination and easy to use and it is suitable for both diagnostic and environmental immunoassays.Type: GrantFiled: April 9, 1997Date of Patent: October 12, 1999Assignee: Oy Medix Biochemica A.B.Inventors: Ilkka Sakari Kouvonen, Eivor Helena Svens, Sari Hannele Tikanoja, Pekka Antero Turunen, Lauri Markus Sivonen
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Patent number: 5965354Abstract: Novel methods and immunodiagnostic test kits for diagnosing herpes simplex virus (HSV) infection are disclosed. The methods and kits employ both type-specific and type-common antigens, in a single-step assay format, to provide for highly accurate results. The methods discriminate between HSV-1 and HSV-2 infection so that an accurate diagnosis can be performed.Type: GrantFiled: March 8, 1996Date of Patent: October 12, 1999Assignee: Chiron CorporationInventors: Rae Lyn Burke, D'Anna Alexander
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Patent number: 5962218Abstract: The invention relates to methods, apparatus, reagents, and kits for performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. In the method, reagents and kits particles can be employed; for instance, for settling upon the electrode surface by gravity, centrifugation or magnetic attraction. The apparatus can include a magnet for generating a magnetic field in a region proximate the electrode.Type: GrantFiled: November 24, 1993Date of Patent: October 5, 1999Assignee: IGEN International Inc.Inventors: Jonathan K. Leland, Haresh P. Shah, John H. Kenten, Jack E. Goodman, George E. Lowke, Gary F. Blackburn, Richard J. Massey
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Patent number: 5962338Abstract: A method of using a reaction plenum in the presence or absence of paramagnetic beads to perform chemical syntheses resulting in separation and recovery of a final desired reaction product is provided. The reaction plenum comprises a reaction plate, a reaction vessel for mounting in the reaction plate, a reaction plate holder, a screw-like rod, a mounting block attached to the reaction plate holder for operably receiving the screw-like rod, a motor, a sonication region in a temperature controllable water bath and a magnetic separation region in the water bath. Paramagnetic beads having reaction sites are introduced into the reaction vessels along with the appropriate solvent and one or more reactants. The contents of the reaction vessel are sonicated, then moved to the magnetic separation region where the paramagnetic beads are tightly held against the reaction vessel while the solvent is aspirated off. Once the desired reaction product is attained, the reaction product is cleaved off the paramagnetic bead.Type: GrantFiled: May 29, 1998Date of Patent: October 5, 1999Assignee: Solid Phase Sciences Corp.Inventor: Irving Sucholeiki
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Patent number: 5962289Abstract: Fusion proteins or conjugates are provided containing an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that binds to a .beta.-1,4-glycan matrix such as cellulose. The substrate binding region is essentially without polysaccharidase activity. In the fusion protein, the substrate binding region is fused or chemically linked to a polypeptide such as an enzyme, a hormone, an immunoglobulin or a protein dye. By contacting the fusion protein with a .beta.-1,4-glycan matrix, the substrate binding region binds to the matrix to immobilize the polypeptide on the matrix. The polypeptide or fusion protein can be removed from the matrix with a protease acting on a protease recognition sequence or with a solution having a low ionic strength or high pH. In the conjugate, the substrate binding region is joined such as by covalent bonding to a non-protein chemical moiety such as a dye, chromophore, fluorescor, radionuclide or enzyme co-factor.Type: GrantFiled: January 24, 1997Date of Patent: October 5, 1999Assignee: The University of British ColumbiaInventors: Douglas G. Kilburn, Robert C. Miller, Richard A.J. Warren, Neil R. Gilkes
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Patent number: 5958791Abstract: A test device for detecting or determining an analyte in a test solution includes an absorbent material having contact, liposome lysing and electrochemical measurement portions. The contact portion is positioned for contact with and uptake of the test solution. The liposome lysing portion is segregated from the contact portion and has a liposome lysing agent bound thereto. The liposome lysing portion is further either positioned between the contact portion and the electrochemical measurement portion, or partially or completely coincides with the electrochemical measurement portion. The electrochemical measurement portion comprises a first conductor comprising a plurality of fingers disposed on the absorbent material, and a second conductor similarly comprising a plurality of fingers disposed on the absorbent material, where the fingers of the first and second conductors are interdigitated to form an array.Type: GrantFiled: September 27, 1996Date of Patent: September 28, 1999Assignee: Innovative Biotechnologies, Inc.Inventors: Matthew A Roberts, Richard Allen Durst, Richard A. Montagna, Geoffrey S. Rule
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Patent number: 5958790Abstract: The present invention relates to a method and kit for carrying out a qualitative or semi-quantitative assay. The invention uses a multi-layer device including an an uppermost cover layer of a water-impermeable material having a hole therein with a diameter of approximately 1-5 mm, an intermediate insoluble porous support layer having a first substance bound thereon in a reaction zone, the hole exposing at least a part of the reaction zone, and a layer of a hydrophilic material in contact with and positioned on the side of the insoluble porous support layer opposite the side with the cover layer. The device permits transverse, but not substantial radial, diffusion of liquid through the reaction zone. Approximately 1-50 .mu.l of a test sample and a second substance are applied to the reaction zone through the hole. A colloidal gold label is attached to the second substance.Type: GrantFiled: May 22, 1995Date of Patent: September 28, 1999Assignee: Nycomed Imaging ASInventor: Erich H. Cerny
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Patent number: 5948696Abstract: Combinatorial chemical libraries of the Formula ?S!-C(O)-L'-Z containing biaryl amino acids are disclosed, in which ?S! represents a solid support and -L'-Z is a linker/compound residue. In these libraries, Z is --NR.sup.1 R.sup.2. R.sup.1 is chosen from H, C.sub.1 to C.sub.20 hydrocarbon, heteroaralkyl, heterocycloalkyl, substituted arylalkyl, alkoxyalkyl or alkyl-SO.sub.2 NH-alkyl and R.sup.4 is an acyl or sulfonyl residue. R.sup.2 is chosen from ##STR1## The combinatorial libraries are optionally encoded with tags. The use of these libraries in assays to discover biologically active compounds is also disclosed.Type: GrantFiled: June 16, 1997Date of Patent: September 7, 1999Assignee: Pharmacopeia, Inc.Inventors: Roland Ellwood Dolle, III, Brian Francis McGuinness, Zahid Hussain
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Patent number: 5948695Abstract: A strip for testing for the presence of an analyte generally comprises a support member which contains a spreading layer and a reagent layer, and a capillary tube in communication with the support layer and spreading layer for transporting a sample of body fluid thereto. A method of testing a fluid for the presence or concentration of an analyte is also provided which generally includes providing a test strip with a support member, a spreading layer, and a reagent layer on the spreading layer. A capillary tube is provided on the support member whereby a fluid containing an analyte to be tested is introduced into the tube and flows through the tube to the spreading layer and contacts the reagent layer.Type: GrantFiled: June 17, 1997Date of Patent: September 7, 1999Assignee: Mercury Diagnostics, Inc.Inventors: Joel S. Douglas, Ryszard Radwanski, Brent G. Duchon, John H. Priest, David A. Hasker, John M. Gleisner
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Patent number: 5945520Abstract: Disclosed are rationally designed mixed mode resins which are useful in recovering a target compound from an aqueous solution and methods for use of such resins. The resins described herein have a hydrophobic character at the pH of binding of the target compound and a hydrophilic and/or electrostatic character at the pH of desorption of the target compound from the resin and are specifically designed to bind the target compound from an aqueous solution at both a low and high ionic strength.Type: GrantFiled: July 22, 1997Date of Patent: August 31, 1999Assignee: Massey UniversityInventors: Simon C. Burton, Neill Ward Haggarty, David R. K. Harding, Nathaniel Todd Becker, Ben A. Bulthuis, Landon M. Steele
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Patent number: 5945345Abstract: The present invention provides a filter for effectively removing substances from a sample of bodily fluid which can interfere with the results of an assay. The filter includes a solid phase support and an active chemical component for binding to the interfering substance. The active chemical component is insoluble in the sample and is immobilized on the solid phase support. The present invention also includes a filter device, transport matrix, assay device, and method for removing substances which interfere with determining the presence of one or more analytes in a sample.Type: GrantFiled: August 27, 1996Date of Patent: August 31, 1999Assignee: Metrika, Inc.Inventors: Joel M. Blatt, Wilma M. Mangan, Paul J. Patel, Victor A. Manneh
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Patent number: 5939332Abstract: Novel analogs of phencyclidine (PCP) are disclosed. The analogs are capable of reacting with anti-PCT antibodies and are useful in immunoassays.The following analogs are described: ##STR1## wherein wherein X is --OH or a reporter molecule selected from the group consisting of aminofluorescein and aminomethylfluorescein and n is an integer between 1 and 10, inclusive.Type: GrantFiled: February 27, 1998Date of Patent: August 17, 1999Assignee: Roche Diagnostics Corp.Inventors: James C. Lee, Luis Fernando Del Corral, Richard A. Clark, Pedro A. Bonilla
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Patent number: 5935779Abstract: The invention relates to methods of performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. Particles are employed in the method, which are then collected in a zone at which electrochemiluminescence can be induced, wherein the amount of induced electrochemilurninescence is related to the amount of analyte in the sample.Type: GrantFiled: November 30, 1994Date of Patent: August 10, 1999Assignee: IGEN International Inc.Inventors: Richard J. Massey, Jonathan K. Leland, Haresh P. Shah, John H. Kenten, Jack E. Goodman, George E. Lowke, Yuzaburo Namba, Gary F. Blackburn