Alpha Or Beta Amino Acid Patents (Class 436/90)
  • Patent number: 10151697
    Abstract: A two-color fluorescence localized super-resolution biological microscopy method and system are disclosed. The method includes: performing two-color fluorescence labeling on a biological sample by using Alexa647 and Alexa750 fluorescent molecules or Cy5 and Cy7 fluorescent molecules, and soaking the biological sample in an imaging buffer solution; illuminating the biological sample by laser to generate a flashing fluorescent signal of a first channel and a flashing fluorescent signal of a second channel respectively; constructing a super-resolution image of a first biological structure and a super-resolution image of a second biological structure respectively; and aligning the super-resolution image of the first biological structure and the super-resolution image of the second biological structure so as to construct a super-resolution image of a third biological structure.
    Type: Grant
    Filed: September 7, 2015
    Date of Patent: December 11, 2018
    Assignee: Shenzhen NanoBioImaging Limited
    Inventors: Teng Zhao, Michael M. T. Loy, Shengwang Du
  • Publication number: 20150079623
    Abstract: The apparatus for analyzing a disease sample according to the present invention includes: a member for separating and quantitatively determining an amino acid stereoisomer in a biological material from a subject; a member for obtaining a disease state index value through a calculation by substituting the amount of the amino acid stereoisomer into a discriminant equation; and a member for outputting disease state information on the subject on the basis of the disease state index value. The method for analyzing a disease sample according to the present invention includes: a step of measuring the amount of amino acid stereoisomers in a biological material from a subject; a step of obtaining a disease state index value through a calculation by substituting the amount of the amino acid stereoisomers into a discriminant equation; and the like.
    Type: Application
    Filed: March 18, 2013
    Publication date: March 19, 2015
    Inventors: Kenji Hamase, Yousuke Toujo, Masashi Mita, Chieko Mizumoto
  • Publication number: 20150080263
    Abstract: The present invention is directed to methods for predicting a pregnant woman's risk of carrying a fetus with Down syndrome (Trisomy 21) or Trisomy 18. The methods are based on measuring one or more metabolites obtained from a pregnant woman's bodily fluid, such as blood or urine, and found to be predictive of Trisomy 21 or Trisomy 18.
    Type: Application
    Filed: April 12, 2013
    Publication date: March 19, 2015
    Inventors: Ray O. Bahado-Singh, Kypros Nicolaides
  • Publication number: 20150072043
    Abstract: Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using 9-oxo-HODE as biomarker.
    Type: Application
    Filed: September 19, 2014
    Publication date: March 12, 2015
    Inventors: Sebastiano Collino, Ivan Montoliu Roura, Francois-Pierre Martin, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150065460
    Abstract: Biomarkers relating to pancreatic Beta-cell function, pancreatic Beta-cell glucose sensitivity, insulin resistance, and/or pancreatic Beta-cell-related disorders are provided. Methods based on the same biomarkers are also provided.
    Type: Application
    Filed: September 11, 2012
    Publication date: March 5, 2015
    Inventors: Walter Gall, Kay A. Lawton
  • Publication number: 20150064792
    Abstract: An external standard solution for use in the analysis of amino acid in plasma, containing, (1) at least one amino acid selected from the following components A, at a concentration of 0.0007 M to 0.49 M, and (2) (i) at least one amino acid selected from the following components B, at a concentration of 0.2 to 0.9 times of the lowest-concentration amino acid among the amino acids selected from components A, (ii) at least one amino acid selected from the following components C, at a concentration of 0.1 to 0.4 times of the lowest-concentration amino acid among amino acids selected from the components A, or (iii) at least one amino acid selected from the following components D, at a concentration of 0.05 to 0.
    Type: Application
    Filed: July 19, 2012
    Publication date: March 5, 2015
    Inventors: Masako Hayakawa, Kazutaka Shimbo, Hiroo Yoshida
  • Publication number: 20150064797
    Abstract: A method for quantitatively analyzing cysteine and cysteine includes a first step of adding a methyl-sulfurating agent to a sample that includes cysteine and cystine to obtain a methyl-sulfurated cysteine, a second step of adding a derivatizing agent to the methyl-sulfurated cysteine and the cystine to obtain a cysteine derivative and a cystine derivative, respectively, and a third step of quantifying the cysteine derivative and the cystine derivative.
    Type: Application
    Filed: August 29, 2014
    Publication date: March 5, 2015
    Inventors: Masashi MITA, Kenji HAMASE
  • Publication number: 20150038522
    Abstract: To provide an anti-cancer agent sensitivity determination marker, which marker can determine whether or not the patient has a therapeutic response to the anti-cancer agent, and novel cancer therapeutic means employing the marker. The anti-cancer agent sensitivity determination marker, the anti-cancer agent including oxaliplatin or a salt thereof and fluorouracil or a salt thereof, contains one or more substances selected from among an amino-acid-metabolism-related substance, a nucleic-acid-metabolism-related substance, a substance in the pentose phosphate pathway, a substance in the glycolytic pathway, a substance in the TCA cycle, a polyamine-metabolism-related substance, 7,8-dihydrobiopterin, 6-phosphogluconic acid, butyric acid, triethanolamine, 1-methylnicotinamide, NADH, NAD+, and a substance involved in the metabolism of any of these substances.
    Type: Application
    Filed: February 22, 2013
    Publication date: February 5, 2015
    Applicants: KEIO UNIVERSITY, KABUSHIKI KAISHA YAKULT HONSHA
    Inventors: Yusuke Tanigawara, Akito Nishimuta, Junya Tsuzaki, Hiroyuki Takahashi
  • Publication number: 20150031572
    Abstract: The present invention pertains to the field of diagnostics for neuronal toxicity and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing neuronal toxicity. It also relates to a method for determining whether a compound is capable of inducing such neuronal toxicity in a subject and to a method of identifying a drug for treating neuronal toxicity. Furthermore, the present invention relates to a device and a kit for diagnosing neuronal toxicity.
    Type: Application
    Filed: February 15, 2013
    Publication date: January 29, 2015
    Applicant: BASF SE
    Inventors: Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Hennicke Kamp, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20150018244
    Abstract: The present invention pertains to the field of diagnostics for hyperthyroidism and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing hyperthyroidism. It also relates to a method for determining whether a compound is capable of inducing such hyperthyroidism in a subject and to a method of identifying a drug for treating hyperthyroidism. Furthermore, the present invention relates to a device and a kit for diagnosing hyperthyroidism.
    Type: Application
    Filed: March 7, 2013
    Publication date: January 15, 2015
    Applicant: BASF SE
    Inventors: Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Hennicke Kamp, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20150010938
    Abstract: Probes for selectively detecting compounds comprising a thiol group and an amino group (“thiols”) are disclosed. Exemplary thiols include cysteine, homocysteine, and glutathione. Embodiments of the disclosed probes produce a detectable change in absorbance spectrum and/or emission spectrum when reacted with one or more thiols in solution. Methods and kits for performing the detection also are disclosed. The probes have a general formula where each bond depicted as “” is a single or double bond; R1, R3-R6 and R8 independently are hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen; R2 is an ?,?-unsaturated aliphatic ester; R7 is oxygen, sulfur, hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen, or R7 and R8 together form a cycloalkyl or aryl ring; X1 is CH2, S, NH, O, Se, Si(CH3)2, Ge(CH3)2, Sn(CH3)2, or C(CH3)2; and X2 is CH, CH2, N, NH, or CR9 where R9 is aryl.
    Type: Application
    Filed: February 22, 2013
    Publication date: January 8, 2015
    Applicant: Oregon State Board of Higher Education on Behalf of Portland State University
    Inventors: Robert M. Strongin, Yixing Guo, Lovemore Hakuna, Mark Allen Lowry, Jorge Omar Escobedo Córdova
  • Publication number: 20150011423
    Abstract: The present invention pertains to the field of diagnostics for kidney toxicity and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing kidney toxicity. It also relates to a method for determining whether a compound is capable of inducing such kidney toxicity in a subject and to a method of identifying a drug for treating kidney toxicity. Furthermore, the present invention relates to a device and a kit for diagnosing kidney toxicity.
    Type: Application
    Filed: September 14, 2012
    Publication date: January 8, 2015
    Applicant: BASF SE
    Inventors: Hennicke Kamp, Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20140308753
    Abstract: Provided herein are methods of diagnosing Alzheimer's disease (“AD”) based on characteristic changes of the levels of certain free amino acids or dipeptides (collectively termed as “AD diagnosis markers”) in the body fluid sample of an individual, carnosine synthesis activities in the plasma, and dopamine synthesis activities in the plasma. Also provided are methods of simultaneously determining the levels of at least two free amino acids or dipeptides in the biological fluid sample of an individual.
    Type: Application
    Filed: January 13, 2014
    Publication date: October 16, 2014
    Applicant: HUNTINGTON MEDICAL RESEARCH INSTITUTES
    Inventors: Alfred N. FONTEH, Michael G. HARRINGTON
  • Publication number: 20140273253
    Abstract: The invention is generally directed to a system and process for fluorometrically quantifying potentially mineralizable nitrogen for agricultural crop production. The soil analysis process measures potentially mineralizable nitrogen and calibrates the application of soil-based nitrogen for site or field specific management of nitrogen fertilizers for crops grown on a wide variety of soil textures including sandy loam, silt loam and clay soils. The spectrofluorometric system and process may be utilized for routine soil testing with a lower sample to sample variability, and the automation of the spectrofluorometric system and process allows for simultaneous determination of potentially mineralizable soil organic nitrogen, ammonium and nitrate.
    Type: Application
    Filed: March 13, 2014
    Publication date: September 18, 2014
    Inventors: Trenton L. Roberts, Richard J. Norman, Nathan A. Slayton, Charles E. Wilson, JR.
  • Publication number: 20140256051
    Abstract: Methods and apparatuses of the present disclosure provide accurate and precise radiation dosimetry based on decarboxylation of a reactant upon exposure to ionizing radiation. The decarboxylation reaction yields carbon dioxide and stable end products, which can be detected and measured, and correlated to determine an amount of radiation exposure. The reactants include carboxyl acids, such as amino acids and others occurring naturally in mammals. The dosimetry may be performed in real-time or retrospectively by assaying a sample of tissue from a patient. Also disclosed is a device which detects and measures radiation dosages based on decarboxylation of a reactant contained therein.
    Type: Application
    Filed: March 6, 2014
    Publication date: September 11, 2014
    Applicant: University of Rochester
    Inventors: William A. Bernhard, Richard M. Watson, Paul Black
  • Publication number: 20140147400
    Abstract: The present invention relates to the use of fish skin as novel industrial source of collagen. Advantageously, said skin is obtained after the filleting or cutting of the fresh fish and frozen immediately after filleting/cutting, thus guaranteeing a very good quality of the base material, both from the bacteriological standpoint and from the standpoint of the native property of the protein.
    Type: Application
    Filed: January 31, 2014
    Publication date: May 29, 2014
    Inventors: Jamilah Bakar, Umi H. Razali, Dzulkifly M. Hashim, Awis Q. Sazili, Kaur Harvinder
  • Publication number: 20140127820
    Abstract: Embodiments of compounds for selectively detecting an analyte are disclosed, along with methods and kits for detecting analytes with the compounds. The compounds are bridged viologen conjugates including at least one fluorophore according to the general structure At least one of R1/R2, R2/R3, R3/R4, R5/R6, R6/R7, and/or R7/R8 together form a substituted or unsubstituted cycloalkyl or aryl.
    Type: Application
    Filed: June 29, 2012
    Publication date: May 8, 2014
    Applicant: The Oregon State Board of Higher Education on Behalf of Portland State University
    Inventors: Robert Michael Strongin, Martha Sibrian-Vazquez, Jorge Omar Escobedo-Cordova, Mark Allen Lowry
  • Publication number: 20140080140
    Abstract: Methods for screening for neurological disorders are disclosed. Specifically, methods are disclosed for screening for neurological disorders in a subject by analyzing a tissue sample obtained from the subject for the presence of elevated levels of neurotoxic amino acids or neurotoxic derivatives thereof associated with neurological disorders. In particular, methods are disclosed for diagnosing a neurological disorder in a subject, or predicting the likelihood of developing a neurological disorder in a subject, by determining the levels of ?-N-methylamino-L-alanine (BMAA) in a tissue sample obtained from the subject. Methods for screening for environmental factors associated with neurological disorders are disclosed. Methods for inhibiting, treating or preventing neurological disorders are disclosed.
    Type: Application
    Filed: September 20, 2013
    Publication date: March 20, 2014
    Inventors: Paul Alan Cox, Sandra Banack, Susan Murch
  • Publication number: 20140045204
    Abstract: Methods of analyzing glatiramer acetate (GA) or a polymeric precursor thereof are provided. The methods can include determining a level of one or more diethylamide-modified amino acids in a sample comprising GA or a polymeric precursor thereof, and selecting at least a portion of the sample based on the assessment of the one or more diethylamide-modified amino acids in the sample.
    Type: Application
    Filed: September 6, 2013
    Publication date: February 13, 2014
    Applicant: Momenta Pharmaceuticals, Inc.
    Inventors: Cuihua Liu, Shiming Dong, Xiao-Jin Xu, Jonathan C. Lansing, Yanjie Jiang
  • Publication number: 20130323849
    Abstract: The invention relates to a material, process and method for the selective analysis of small molecules. Particularly the invention provides a material and a technique for the analysis of small molecules excluding other large molecular weight (MW) analytes. The process involves selective detection of low molecular weight molecules from a sample comprising the steps of placing said sample with SBA-15 particles; and subjecting the same to desorption ionization mass spectrometry, wherein low molecular weight molecules are selectively detected over the higher molecular weight molecules. A kit for the selective analysis of small molecules is also provided.
    Type: Application
    Filed: February 17, 2012
    Publication date: December 5, 2013
    Applicants: COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH, JAWAHARLAL NEHRU UNIVERSITY
    Inventors: Dipankar Ghosh, Venkateswarlu Panchagnula, Deepika Dhaware
  • Patent number: 8501488
    Abstract: The present invention relates to a compound for N-terminal substitution of polypeptides which is used in sequencing and quantifying amino acids and a method for sequencing and quantifying an amino acid sequence using the same. The method for sequencing and quantifying amino acids in accordance with the present invention leads to a relative quantitative analysis of proteins with very high reliability, and can distinctively discriminate between y-type ions and b-type ions on the MS/MS spectra, providing the means for realization of high-reliability protein identification.
    Type: Grant
    Filed: June 29, 2005
    Date of Patent: August 6, 2013
    Assignee: Korea University Industry and Academy Cooperation Foundation
    Inventors: Sang-Won Lee, Yong-Ho Lee
  • Patent number: 8455265
    Abstract: A surface grafted conjugated polyelectrolyte (CPE) is formed by coupling a CPE by a coupling moiety to the surface of a substrate. The substrate can be of any shape and size, and for many uses of the surface grafted CPE, it is advantageous that the substrate is a nanoparticle or microparticle. Surface grafted CPEs are presented that use silica particles as the substrate, where a modified silane coupling agent connects the surface to the CPE by a series of covalent bonds. Two methods of preparing the surface grafted CPEs are presented. One method involves the inclusion of the surface being modified by the coupling agent and condensed with monomers that form the CPE in a grafted state to the substrate. A second method involves the formation of a CPE with terminal groups that are complimentary to functionality that has been placed on the surface of the substrate by reaction with a coupling agent. The surface grafted CPEs are also described for use as biosensors and biocides.
    Type: Grant
    Filed: February 29, 2008
    Date of Patent: June 4, 2013
    Assignee: STC.UNM
    Inventors: David G. Whitten, Sireesha Chemburu, Thomas Corbitt, Linnea Ista, Gabriel Lopez, Kirk S. Schanze, Motokatsu Ogawa, Eunkyung Ji
  • Publication number: 20130023056
    Abstract: A monitoring test for recurrent breast cancer with a high degree of sensitivity and specificity is provided that detects the presence of a panel of multiplicity of biomarkers that were identified using metabolite profiling methods. The test is capable of detecting breast cancer recurrence about a years earlier than current available monitoring diagnostic tests. The panel of biomarkers is identified using a combination of nuclear magnetic resonance (NMR) and two dimensional gas chromatography-mass spectrometry (GC×GC-MS) to produce the metabolite profiles of serum samples. The NMR and GC×GC-MS data are analyzed by multivariate statistical methods to compare identified metabolite signals between samples from patients with recurrence of breast cancer and those from patients having no evidence of disease.
    Type: Application
    Filed: September 21, 2012
    Publication date: January 24, 2013
    Applicant: PURDUE RESEARCH FOUNDATION
    Inventor: PURDUE RESEARCH FOUNDATION
  • Patent number: 8278111
    Abstract: Compositions comprising a tripeptide having the sequence XC1C2; wherein X is any amino acid such that XC1C2 is capable of binding a metal in a square planar orientation or square pyramidal orientation or both; and wherein C1 and C2 are the same or different; and wherein C1 and C2 individually are chosen from a cysteine and a cysteine-like nonnatural amino acid, as well as metal-XC1C2 complexes and methods for forming such complexes.
    Type: Grant
    Filed: December 30, 2011
    Date of Patent: October 2, 2012
    Assignee: University of Kansas
    Inventors: Jennifer Ann Stowell Laurence, Anthony Andrew Vartia, Mary Elizabeth Krause
  • Patent number: 8252596
    Abstract: Disclosed are methods for treating a subject suffering from phenylketonuria and/or phenylalanemia. The methods include, in part, enterally administering to the subject a LNAA supplement in which the weight ratio of Leu to Val is greater than 2:1; in which the weight ratio of Leu to iLeu is greater than 3:1; or which includes one or more LNAAs and which further includes Lys. LNAA supplements are also disclosed. Also disclosed are methods for treating a subject suffering from a condition involving a metabolic disorder involving the metabolism of a first amino acid X. The method includes enterally administering to the subject a composition which (i) is substantially free from the first amino acid X and (ii) which includes a second amino acid Y that competes with amino acid X at a gastrointestinal tract transporter.
    Type: Grant
    Filed: August 5, 2010
    Date of Patent: August 28, 2012
    Assignee: PreKUlab Ltd.
    Inventor: Reuben Matalon
  • Patent number: 8247236
    Abstract: Methods of evaluating molecular isomers of branched-chain amino acids are featured. The methods can include: derivatizing one or more molecular isomers of branched-chain amino acids in a sample comprising a branched-chain amino acid labeled with one or more heavy atoms as a first standard; adding, to the sample, after derivatization, a nonderivatized or derivatized branched chain amino acid that is labeled with one or more heavy atoms, as a second standard; evaluating the sample using tandem mass spectrometry; and detecting peaks indicative of derivatized and nonderivatized forms of one or more branched-chain amino acids in the sample.
    Type: Grant
    Filed: April 11, 2011
    Date of Patent: August 21, 2012
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventors: Blas Cerda, Alex Cherkasskiy, Yijun Li
  • Publication number: 20120197539
    Abstract: A method for assessing patient health is provided using metabolomics. The method comprises providing a bodily fluid or tissue sample from a subject, collecting a metabolic profile from the bodily fluid or tissue sample and comparing the metabolic profile to a reference profile, wherein the preferred bodily fluid is urine. Reference profiles are also provided.
    Type: Application
    Filed: October 12, 2010
    Publication date: August 2, 2012
    Inventor: Carolyn Slupsky
  • Publication number: 20120190119
    Abstract: A method for establishing a treatment for promoting the formation of Vitamins in an individual. The measured or assessed level of substances in the body are compared in order to determine the treatment to provide to an individual.
    Type: Application
    Filed: January 25, 2011
    Publication date: July 26, 2012
    Inventor: WILLIAM J. McFAUL
  • Publication number: 20120077711
    Abstract: The present invention provides compounds and libraries of compounds having formula (I): wherein L, n, S and A are defined generally and subsets herein. These compounds and libraries of compounds are useful generally in the drug discovery process.
    Type: Application
    Filed: May 26, 2011
    Publication date: March 29, 2012
    Applicant: SUNESIS PHARMACEUTICALS, INC.
    Inventors: Andrew C. Braisted, Daniel A. Erlanson, Jeffrey W. Jacobs
  • Patent number: 8119411
    Abstract: The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the
    Type: Grant
    Filed: December 20, 2010
    Date of Patent: February 21, 2012
    Assignee: NEC Corporation
    Inventors: Kenji Miyazaki, Akira Tsugita, Kenichi Kamijo, Hiroaki Torii
  • Publication number: 20120040383
    Abstract: Embodiments of the present invention provide methods of assessing metabolite biomarkers to diagnose, monitor, prognose and treat colorectal cancer, as well as kits and systems in use thereof. In some embodiments, the metabolite biomarker profile of an subject suspected of having colorectal cancer may be compared to the metabolite biomarker profile of a healthy subject to determine if there are differences between the profiles indicative of colorectal cancer. In some embodiments, the stage of a subject's colorectal cancer may be determined. In certain embodiments, the metabolite biomarker profile of an subject may be useful in determining method of treatment for colorectal cancer. In other embodiments, the metabolite biomarker profiles of an subject having colorectal cancer may be compared prior to and after treatment to determine if there are differences between the profiles indicative of decreased colorectal cancer morbidity.
    Type: Application
    Filed: August 12, 2011
    Publication date: February 16, 2012
    Inventors: Wei Jia, Yunping Qiu
  • Patent number: 8071392
    Abstract: The present invention provides a protein analysis method using a combination of two or more kinds of stable isotopes of a compound represented by the formula (I): wherein R1, R2 and R3 are the same or different and each is hydrogen, halogen or alkyl, or a salt thereof, as a labeling compound to produce a difference in the mass of the same kind of protein contained in each sample.
    Type: Grant
    Filed: June 19, 2008
    Date of Patent: December 6, 2011
    Assignees: National University Corporation University of Fukui, Taiyo Nippon Sanso Corporation
    Inventor: Shigeru Matsukawa
  • Patent number: 8021570
    Abstract: The present invention provides materials and methods that make liquid crystal phases accessible with relatively short ?-peptides in aqueous solvents.
    Type: Grant
    Filed: June 13, 2007
    Date of Patent: September 20, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Samuel H. Gellman, Nicholas L. Abbott, William C. Pomerantz
  • Patent number: 8008262
    Abstract: ?-peptide regions of polypeptides can serve as structural mimics of ?-helices in wild type proteins. Because ?-helices of one protein often bind to a target protein in a biological pathway, a polypeptide that contains a helical ?-peptide region can be used to disrupt this type of protein-protein binding. As a result, polypeptides that contain a helical ?-peptide region can be used to treat conditions involving this type of protein-protein binding, such as viral infections and cell proliferation.
    Type: Grant
    Filed: April 21, 2005
    Date of Patent: August 30, 2011
    Assignee: Yale University
    Inventors: Alanna Schepartz Shrader, Scott A. Hart, Joshua A. Kritzer, Olen M. Stephens
  • Publication number: 20110189780
    Abstract: Methods of evaluating molecular isomers of branched-chain amino acids are featured. The methods can include: derivatizing one or more molecular isomers of branched-chain amino acids in a sample comprising a branched-chain amino acid labeled with one or more heavy atoms as a first standard; adding, to the sample, after derivatization, a nonderivatized or derivatized branched chain amino acid that is labeled with one or more heavy atoms, as a second standard; evaluating the sample using tandem mass spectrometry; and detecting peaks indicative of derivatized and nonderivatized forms of one or more branched-chain amino acids in the sample.
    Type: Application
    Filed: April 11, 2011
    Publication date: August 4, 2011
    Applicant: PERKINELMER HEALTH SCIENCES, INC.
    Inventors: Blas Cerda, Alex Cherkasskiy, Yijun Li
  • Publication number: 20110143444
    Abstract: According to the method of evaluating female genital cancer of the present invention, amino acid concentration data on concentration values of amino acids in blood collected from a subject to be evaluated is measured, and the state of female genital cancer including at least one of cervical cancer, endometrial cancer, and ovarian cancer in the subject is evaluated based on the concentration value of at least one of Thr, Ser, Asn, Gln, Pro, Gly, Ala, Cit, Val, Met, Ile, Leu, Tyr, Phe, His, Trp, Orn, Lys, and Arg contained in the measured amino acid concentration data of the subject.
    Type: Application
    Filed: December 15, 2010
    Publication date: June 16, 2011
    Inventors: Takahiko Muramatsu, Yutaka Ihata, Akira Imaizumi, Hiroshi Yamamoto, Etsuko Miyagi, Fumiki Hirahara
  • Publication number: 20110143445
    Abstract: A plurality of mass differential tagging reagents is used to label amine functionality in amine-containing compounds. The labeled analytes have distinct retention times on a reversed phase column, and distinct masses. Under high energy collision, reporter groups can be generated and the intensity or the peak area detected for each reporter group can be used for quantitation. One exemplary set of reagents includes a set of three different mass differential reagents comprising tagging weights of 140 atomic mass units, 144 atomic mass units, and 148 atomic mass units, respectively, with reporter groups of 113, 117, and 121 atomic mass units, respectively. A package including each of the mass differential reagents is also provided and can include separate respective containers, for example, one for each of the different reagents. The package can also include one or more standards each comprising a respective known concentration of a respective known amine-containing compound.
    Type: Application
    Filed: December 15, 2010
    Publication date: June 16, 2011
    Applicant: DH Technologies Development Pte. Ltd.
    Inventors: Scott DANIELS, Subhasish PURKAYASTHA
  • Publication number: 20110111446
    Abstract: It is an object of the present invention to provide: a novel thiol-detecting reagent, which can be used in vivo and which solves the problem regarding the generation of background fluorescence due to hydrolysis; and a method for detecting thiol using the aforementioned reagent. The present invention provides a compound represented by the following formula (1): wherein each R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom.
    Type: Application
    Filed: January 30, 2009
    Publication date: May 12, 2011
    Applicant: RIKEN
    Inventors: Hiroshi Abe, Yoshihiro Ito, Aya Shibata, Mika Ito
  • Publication number: 20110104737
    Abstract: The invention relates to a mixing container (1) for a photometric measuring device, comprising a closing element (2) that can be removed from a filling hole (3) and a first liquid (5) which is located in the interior (4) of the mixing container (1), with the mixing container (1) comprising a dosing element (8) which can be placed on the filling hole (3) of the mixing container (1) after removing the closing element (2) and adding a sample liquid, and with the dosing element (8) containing a second liquid (13) in a closed hollow space (9). In accordance with the invention, the hollow space (9) in the dosing element (8) is closed by a movable plug (10) which is discharged into the interior of the mixing container (1) along with the second liquid (13) once the second liquid (13) has been subjected to pressurization.
    Type: Application
    Filed: October 27, 2010
    Publication date: May 5, 2011
    Inventor: Gerhard Bonecker
  • Patent number: 7932092
    Abstract: A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before ?-aminoisobutyric acid (?-AiBA) is eluted.
    Type: Grant
    Filed: August 22, 2008
    Date of Patent: April 26, 2011
    Assignee: Hitachi, Ltd.
    Inventors: Yoshio Fujii, Masahito Ito, Kimiyoshi Koda
  • Patent number: 7901895
    Abstract: Methods for screening for neurological disorders are disclosed. Specifically, methods are disclosed for screening for neurological disorders in a subject by analyzing a tissue sample obtained from the subject for the presence of elevated levels of neurotoxic amino acids or neurotoxic derivatives thereof associated with neurological disorders. In particular, methods are disclosed for diagnosing a neurological disorder in a subject, or predicting the likelihood of developing a neurological disorder in a subject, by determining the levels of ?-N-methylamino-L-alanine (BMAA) in a tissue sample obtained from the subject. Methods for screening for environmental factors associated with neurological disorders are disclosed. Methods for inhibiting, treating or preventing neurological disorders are disclosed.
    Type: Grant
    Filed: August 13, 2007
    Date of Patent: March 8, 2011
    Assignee: The Institute for Ethnomedicine
    Inventors: Paul A Cox, Sandra Banack, Susan Murch
  • Patent number: 7879616
    Abstract: The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the
    Type: Grant
    Filed: December 25, 2003
    Date of Patent: February 1, 2011
    Assignee: NEC Corporation
    Inventors: Kenji Miyazaki, Akira Tsugita, Kenichi Kamijo, Hiroaki Torii
  • Publication number: 20110003842
    Abstract: Provided are a marker for determining sensitivity to an anticancer agent capable of distinguishing a therapeutic response of an individual patient and a novel means for a cancer therapy using the marker. The marker for determining sensitivity to an anticancer agent contains a substance in a metabolic pathway in which L-phenylalanine and/or N,N-dimethyl glycine are/is involved.
    Type: Application
    Filed: January 30, 2009
    Publication date: January 6, 2011
    Applicants: KEIO UNIVERSITY, KABUSHIKI KAISHA YAKULT HONSHA
    Inventors: Yusuke Tanigawara, Mitsuhiro Watanabe, Eri Arita, Akito Nishimuta, Yasuko Yamayoshi, Takeshi Matsuzaki, Shinji Sugimoto
  • Publication number: 20110003393
    Abstract: The present invention generally relates to influenza and, in particular, to systems and methods for studying influenza viruses such as the influenza A virus. One aspect of the invention is generally directed towards systems and methods for determining the timescale dynamics of the tryptophan residue located in position 41 of the M2 proton channel of the influenza A virus, for instance, via nuclear magnetic resonance. This may be useful, for example, in determining whether a candidate drug is able to alter the dynamics of the tryptophan residue, and thus, whether the drug targets the M2 proton channel.
    Type: Application
    Filed: October 24, 2008
    Publication date: January 6, 2011
    Applicant: President and Fellows of Harvard College
    Inventors: James Chou, Jason R. Schnell
  • Publication number: 20100267005
    Abstract: Crosslinked proteins, proteins and polymers, and polymers and methods of making the same are disclosed. In one illustrative embodiment, a method is provided comprising the steps of attaching a chelator to one or more polymers; creating a coordination complex between the first protein, the second protein, and a metal ion; and crosslinking the first and second proteins by exposing the coordination complex to an oxidant.
    Type: Application
    Filed: August 11, 2004
    Publication date: October 21, 2010
    Applicant: University of Utah Research Foundation
    Inventors: Russell John Stewart, Patrick Franklin Kiser, Richard Scott Staynor
  • Patent number: 7780950
    Abstract: A diagnostic method of monitoring anti-inflammatory and/or antioxidant actions of therapeutic agents comprises determining the level of at least one systemic marker indicative of inflammation or oxidation in a bodily sample taken from a subject at base line or following administration of the therapeutic agent. The marker includes at least one of MPO activity, MPO mass, select MPO-generated oxidation products, and combinations thereof. The level of the systemic marker is compared with a predetermined value to monitor the anti-inflammatory and/or antioxidant actions of the therapeutic agent.
    Type: Grant
    Filed: April 17, 2003
    Date of Patent: August 24, 2010
    Assignee: The Cleveland Clinic Foundation
    Inventor: Stanley L. Hazen
  • Publication number: 20100210023
    Abstract: The present invention provides a novel oral cancer and periodontal disease salivary metabolome for use in the diagnosis or for providing a prognosis for oral cancer and periodontal disease in an individual. The present invention also provides novel methods of diagnosing or providing a prognosis for oral cancer or periodontal disease by detecting metabolites found in the saliva of an individual. Finally, the present invention provides kits for the detection of salivary metabolites useful in the diagnosis or prognosis of oral cancer and periodontal disease in an individual.
    Type: Application
    Filed: September 18, 2009
    Publication date: August 19, 2010
    Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, KEIO UNIVERSITY
    Inventors: David T.W. Wong, Masaru Tomita, Masahiro Sugimoto, Akiyoshi Hirayama, Tomoyoshi Soga
  • Patent number: 7771954
    Abstract: A diagnostic method of monitoring anti-inflammatory and/or antioxidant actions of therapeutic agents comprises determining the level of at least one systemic marker indicative of inflammation or oxidation in a bodily sample taken from a subject at base line or following administration of the therapeutic agent. The marker includes at least one of MPO activity, MPO mass, select MPO-generated oxidation products, and combinations thereof. The level of the systemic marker is compared with a predetermined value to monitor the anti-inflammatory and/or antioxidant actions of the therapeutic agent.
    Type: Grant
    Filed: April 26, 2006
    Date of Patent: August 10, 2010
    Assignee: The Cleveland Clinic Foundation
    Inventors: Stanley L. Hazen, Zeneng Wang
  • Patent number: 7670783
    Abstract: Methods for screening for neurological disorders are disclosed. Specifically, methods are disclosed for screening for neurological disorders in a subject by analyzing a tissue sample obtained from the subject for the presence of elevated levels of neurotoxic amino acids or neurotoxic derivatives thereof associated with neurological disorders. In particular, methods are disclosed for diagnosing a neurological disorder in a subject, or predicting the likelihood of developing a neurological disorder in a subject, by determining the levels of ?-N-methylamino-L-alanine (BMAA) in a tissue sample obtained from the subject. Methods for screening for environmental factors associated with neurological disorders are disclosed. Methods for inhibiting, treating or preventing neurological disorders are disclosed.
    Type: Grant
    Filed: June 8, 2007
    Date of Patent: March 2, 2010
    Assignee: The Institute for Ethnomedicine
    Inventors: Paul A Cox, Sandra Banack, Susan Murch
  • Patent number: 7670841
    Abstract: The present invention provides, as a method of analyzing the C-terminal amino acid sequence of a peptide with use of reaction technique for successively releasing the C-terminal amino acids, in which undesirable side reactions, such as cleavage of a peptide bond at the middle of the peptide, can be prevented and chemical treatments therein can be carried out under widely applicable conditions in the course of successive release of the C-terminal amino acids from a peptide, such a method comprising steps of dehydrating the gel on which a target peptide that has been separated by gel electrophoresis is held in the bound state; immersing it in a mixture solution of an alkanoic acid anhydride added with a small amount of a perfluoroalkanoic acid in a dipolar aprotic solvent to re-swell the gel carrier, forming a 5-oxazolone structure, at a temperature chosen in the range of from 30° C. to 80° C.
    Type: Grant
    Filed: November 28, 2003
    Date of Patent: March 2, 2010
    Assignee: NEC Corporation
    Inventors: Kenji Miyazaki, Akira Tsugita, Kenichi Kamijo, Takuji Nabetani