Abstract: The invention provides a stable solid reagent for use in a prepackaged test kit for the detection of cannabinoids comprising a diazonium salt which undergoes characteristic color change when combined with a cannabinoid under basic conditions, the salt comprising an anion capable of causing the entire salt including the diazonium cation thereof to dissolve in an organic solvent when combined therewith to form a stable liquid reagent for the detection of cannabinoids. The invention also provides a stable liquid reagent comprising the aforementioned diazonium salt and an organic solvent as well as providing prepackaged reagent test kits containing the aforementioned reagents and methods for detecting cannabinoids using the reagents of the invention.

Abstract: A fluorescent compound is made from the reaction of naphthoyl chloride and a cardiac glycoside derived from serum or plasma. The method of carrying out the reaction provides a product which allows for a 100-fold increase in sensitivity when the product is subjected to chromatographic analysis according to the described apparatus.

Abstract: A chemiluminescence method for assaying compounds containing primary amino groups using 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole fluorescers is disclosed. The 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole (CBI or CNI) is formed by reacting a compound containing a primary amino group with naphthalene-2-3-dicarboxaldehyde or anthracene-2,3-dicarboxaldehyde in the presence of cyanide ion. The reaction product of hydrogen peroxide with an oxalate ester is then combined with the CBI or CNI to form an analyte which exhibits chemiluminescence. A detector is used to measure the chemiluminescence emission from the chemiluminescence derivatized analytes. Compounds containing primary amino groups which may be assayed according to the present invention include primary amines, amino acids, peptides and catecholamines. The method can also be adapted to the analysis of trace amounts of cyanide ion and hydrogen peroxide.

Abstract: The amount of lipid bound sialic acid in a blood plasma or serum sample may be determined by a method which may be automated involving the following steps: diluting the sample with distilled water; mixing and then cooling the diluted sample; adding a mixture of a chlorinated lower alkyl hydrocarbon and a lower alkyl alcohol; mixing, diluting with water and then treating by mixing further and centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a mixture of a protein precipitating agent and adsorbing material, mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present.

Abstract: Certain substituted benzo- and naphthoquinone electron transfer agents are useful in analytical compositions, elements and methods, e.g. for determinations of living cells. These electron transfer agents are capable of being reduced by an analyte, and the reduced electron transfer agent, in turn, reduces another compound providing a detectable species (e.g. a dye). The reduction potential (E.sub.1/2) of the electron transfer agents useful inthis invention is in the range of from about -320 to about +400 mV as measured in an aqueous buffer solution at pH 7.

Abstract: A method of forming analyzable adducts in a mixture of compounds by contacting the mixture with a boron reagent having the formula of either ##STR1## where each X and Y is, independently, an alkyl group of 12 or fewer carbons or an aryl group of 6-20 carbons; or BZ.sub.3, where each Z is, independently, an alkyl group of 12 or fewer carbons, or an aryl group of 6-20 carbons.

Abstract: The amount of lipid bound sialic acid in a whole blood sample may be determined by a method, which may be automated, involving the following steps: adding to the sample a lower alkyl alcohol and deionized distilled water; mixing the resulting admixture; adding to it a mixture of lower alkyl chlorinated hydrocarbon and a lower alkyl alcohol; treating by mixing and centrifuging the mixture until a recoverable, substantially clear upper phase forms; recovering the upper phase and adding to it a protein-precipitating agent, preferably in admixture with an adsorbing material; mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present. The presently preferred mixture for addition to the upper phase is a mixture of 75% by weight phosphotungstic acid and 25% by weight silica gel on a dry weight basis.

Abstract: An assay for Vitamin B.sub.12 deficiency by determining for a blood sample that essentially all of the Vitamin B.sub.12 is carried by a combination of TCI and TCIII and/or essentially no Vitamin B.sub.12 is carried by TCII. TCII may be conveniently separated from a combination of TCI and TCIII by precipitating TCII with silica. Vitamin B.sub.12 deficiency may be determined prior to the onset of anemia and/or nerve damage by determining a decrease in Vitamin B.sub.12 carried by TCII.

Abstract: An indicator for detecting residual ethylene oxide comprising a substrate impregnated with a solution of 4-(4-nitrobenzyl)pyridine and an envelope of the substrate which is at least partially transparent and has low permeability to gaseous ethylene oxide is provided, the envelope being preferably made of a transparent plastic film. With the indicator, an amount of ethylene oxide remaining in various medical instruments, etc. may be estimated by the naked eye since the color of the indicator changes step by step in the course of the release of residual ethylene oxide.

Abstract: An indicator comprising azo dye(s), epoxy compound(s) and a separating membrane can be utilized for detection of the completion of thermal sterilization treatment in the medical field and also for thermal inspection in such various fields as testing, instrumentation and the like. The indicator can be used also for detection of the completion of alkylene oxide gas sterilization treatment by ethylene oxide gas or the like.

Abstract: The present invention provides stabilized compositions of tetrazolium salts for analytical purposes, wherein they contain 1 to 10 moles of a complex-forming acid which is soluble in polar solvents per mole of tetrazolium salt.The present invention also provides a process for the detection of reducing materials, wherein one of the above stabilized compositions is added to a test batch.

Abstract: A visual indicator device and dosimeter in which a liquid crystal composition deposited on an opaque dark-colored carrier substrate undergoes color changes which depend on the concentration, and total quantity, of ethylene oxide to which the device has been exposed. The device serves both to monitor the concentration of ethylene oxide in the environment and to indicate a time-weighted average exposure.

Abstract: A method and apparatus for determining the composition and the concentration of biocidal gas and other gases contained in a reaction chamber during processing of material contained in the chamber and exposed to the treating gas. A valved pre-evacuated gas-sampling vessel is positioned in the reaction chamber and, thereafter, opened by remote control to receive a gas sample representative of the gaseous atmosphere present in the chamber. The sampling vessel is then closed, removed from the treatment chamber, and connected to apparatus for analyzing the gas in the vessel. The qualitative and quantitative composition of the treating gas is thus determined.

Abstract: The present invention provides a process for removing ascorbic acid from aqueous solutions by the addition of ascorbate oxidase, wherein catalase and hydrogen peroxide are also added to the solution.The present invention also provides an agent for the removal of ascorbic acid from aqueous solutions, based on a solid carrier material impregnated with ascorbate oxidase, wherein the carrier material also contains catalase and hydrogen peroxide and optionally also a stabilizer and/or buffer substance.

Abstract: A two-component liquid reagent and process is provided for detecting the presence of furfural in petroleum products.

Abstract: This invention relates to diagnostic reagents and a method for increasing the sensitivity of chemical and enzymatic analysis. In particular, it relates to an improved reagent and method wherein the sensitivity of the analysis is improved by the addition of a water-soluble inclusion compound.

Abstract: A device for chemically quantifying ethylene oxide exposure is disclosed as are methods for its use. The device comprises an ethylene oxide-permeable layer exhibiting unusual non-Fickian EO transport and containing a controlled amount of compound which forms a colored species upon reaction with ethylene oxide. Optionally, the response layer is laminated or otherwise joined with a support layer. The device can be used to quantify in industry accepted units of time and concentration sterilization dosages of ethylene oxide.

Abstract: A method of quantitatively determining the amount of exposure of an object to ethylene oxide gas. A pre-weighed ethylene oxide-permeable container, which encloses a predetermined amount of catalyst for the reaction of the ethylene oxide gas to form a liquid, is placed adjacent to the object. The object and container are then exposed to ethylene oxide gas for controlled time and at a known humidity and temperature. The container is then reweighed. The weight gain of the container is a quantitative indication of the amount of exposure to ethylene oxide gas. The test system may be calibrated against its effect in known concentrations of ethylene oxide gas, so that the precise amount of exposure to ethylene oxide gas can be determined.

Abstract: A method for detecting fluorescent materials in liquid chromatography. Fluorescent materials are excited by chemical reaction with chemical reagents to yield light (chemiluminescence) and detected at high sensitivity. The detection apparatus is composed of a mixer for mixing a solution containing separated fluorescent materials and solutions of chemical reagents to afford chemiluminescence, a flow cell and a light receptive detection means for detecting the light thus produced.

Abstract: A sensing apparatus and method for qualitatively and quantitatively analyzing small amounts of selected oxidizable gases in a carrier gas including the associated electronic circuitry is described. The apparatus preferably includes two semi-conductor type sensing elements in electrical parallel so that the difference in resistance of the two sensors can be detected, which can be of the N or P type and which decrease or increase in electrical resistance as a function of the concentration of an oxidizable gas. Also, a method for determining fruit maturity and ripeness by the detection of ethylene in the internal atmosphere of a fruit is described which preferably uses the apparatus with the semi-conductor type sensing elements, but can use other variable resistance type gas sensing means.

Abstract: An indicator system and device for visually monitoring ethylene oxide sterilization by color changes of the indicating composition of the system upon sufficient and properly conditioned exposure of the composition to ethylene oxide provides a controlled method for indicating the effective sterilization of articles by ethylene oxide. The indicating composition of this invention undergoes color changes that are progressive with the conditions and periods of sterilization, such that a final and complete color change indicates the completion of an effective ethylene oxide sterilization. The indicating composition comprises a leuco precursor of an aryl methane dye selected from the groups herein defined; and an acidic constituent. Acidic organic compounds such as diphenolic acid (4,4-bis [4-hydroxyphenyl] pentanoic acid) are effective acid constituents because they enhance hue, develop color and stabilize the final color change.