Abstract: A method for functionalising a substrate intended for the self-assembly of a block copolymer, includes depositing on the surface of a substrate a layer of a first polymer material, the first polymer having a first chemical affinity with respect to the block copolymer; grafting one part only of the first polymer material layer onto the surface of the substrate; printing, using a mould, patterns in a sacrificial layer arranged above the grafted part of the first polymer material layer; transferring the patterns of the sacrificial layer into the grafted part of the first polymer material layer, until the substrate is reached; and removing at least one part of the sacrificial layer by wet etching, so as to uncover the grafted part of the first polymer material layer.

Abstract: Provided is a use of an aggregation-induced emission compound in dispersion detecting of nanoparticles. The dispersion detecting of nanoparticles includes modifying the aggregation-induced emission compound on the surfaces of the nanoparticles to obtain a modified nanoparticles solution. The dispersion detecting of nanoparticles includes exciting the modified nanoparticles solution and determining the dispersion of the nanoparticles by the luminescence state of the solution.

Abstract: A method for forming contact vias includes providing a substrate comprising a plurality of contact structures embedded in a first dielectric layer, the contacts abutting an upper surface of the first dielectric layer. The method also includes providing a second dielectric layer on the upper surface of the first dielectric layer, and providing contact vias in the second dielectric layer by patterning the second dielectric layer at least at positions corresponding to the contact structures, wherein patterning the second dielectric layer comprises using a DSA patterning technique.

Abstract: A method including forming a line pattern in a substrate includes using a plurality of longitudinally spaced projecting features formed along respective guide lines as a template in forming a plurality of directed self-assembled (DSA) lines that individually comprise at least one of (a): the spaced projecting features and DSA material longitudinally there-between, and (b): are laterally between and laterally spaced from immediately adjacent of the guide lines. Substrate material elevationally inward of and laterally between the DSA lines may be processed using the DSA lines as a mask.

Abstract: A bead construct includes a bead, a fluorophore molecule, first and second linker molecules and a ligand molecule. The fluorophore molecule comprises at least two subunits connected to each other by at least one internal linker that provides conformational freedom to the at least two subunits. An optical signal emitted by the fluorophore molecule changes when reducing the conformational freedom of the at least two subunits to each other. The first linker molecule is connected to the bead and to one of the subunits of the flurophore molecule. The second linker molecule is connected to another one of the subunits of the fluorophore molecule. The ligand molecule is connected to the second linker molecule.

Abstract: Examples of integrated sensors are disclosed herein. An example of an integrated sensor includes a substrate and a sensing member formed on a surface of the substrate. The sensing member includes collapsible signal amplifying structures and an area surrounding the collapsible signal amplifying structures that enables self-positioning of droplets exposed thereto toward the collapsible signal amplifying structures.

Abstract: Provided are methods and compositions for immunoassay with improved specificity. The presently disclosed bead-based blocking agents reduce the interference associated with the samples and reagents of such assays.

Abstract: In one aspect, described herein are field effect chemical sensor devices useful for chemical and/or biochemical sensing. Also provided herein are methods for single molecule detection. In another aspect, described herein are methods useful for amplification of target molecules by PCR.

Abstract: Surface modifications to sensors in an array give the sensors different functionalities for adsorbing or binding molecules. A first sensor in the array includes a first resonating member having a first surface comprising a polymer receptor material coated over a first underlying material. A second sensor includes a second resonating member having a second surface comprising the polymer receptor material coated over a second underlying material that is different than the first underlying material. The first underlying material, the second underlying material, and the polymer receptor material are selected such that the first resonating member, having a combination of the polymer receptor material and the first underlying material, has a different ability to adsorb or bind a mass of one or more analytes than does the second resonating member having a combination of the polymer receptor material with the second underlying material. Methods for fabricating sensors with surface modifications are also provided.

Abstract: A system and methods for consumer use of a microarray product are provided. A method includes obtaining a microarray product and contacting the microarray product with a sample and performing bioassays. The method further includes obtaining a decoding information data package from a manufacturer of the microarray product for correlating bioassay signals from the bioassays with respective analytes, wherein the microarray product is obtained at a first time different and discrete from a second time that the decoding information data package is obtained.

Abstract: A muitimode gas sensor platform (100) can comprise an array of electrode pairs (108) oriented on a substrate (102) and a plurality of detection zones (104), wherein at least a portion of individual electrode pairs (106) are separately addressable. Each detection zone (104) can comprise at least one set of individual electrode pairs (106) within the array, where the individual electrode pairs (106) have organic nanofibers (108) uniformly deposited thereon. The organic nanofibers (108) can be responsive to association with a corresponding target material and at least one detection zone (104) can be electronically responsive to the corresponding target material.

Abstract: In a method and system for forming concentrated volumes of microbeads, a polymer solution and/or suspension includes a polymer dissolved and/or dispersed in a medium. Streams of a focusing fluid and of the polymer solution and/or suspension flow towards a fluid bath, and into intersection with one another, so as to focus the polymer solution and/or suspension. The polymer solution and/or suspension stream forms microbeads in the fluid bath Some of the focusing fluid is drawn from the fluid bath, so as to concentrate the microbeads in the fluid bath. The system includes a flow focusing apparatus and a liquid-containing cell. The focusing apparatus includes polymer and focusing nozzles. The cell contains the fluid bath and has an outlet port, through which the focusing fluid is drawn.

Abstract: Polymer compositions having the chemical structure: as well as monomer compositions for producing said polymers are described. Methods for preparing these polymers and combinatorial libraries of these polymers are also described.

Abstract: The present invention relates to a device for isolating and recovering a biomolecule from a test sample. The device includes a support and at least one peelable layer deposited on at least a portion of the support. The peelable layer includes a substrate having a target component immobilized on the substrate. The device is effective for isolating and recovering a biomolecule having affinity to the target component. The present invention also relates to systems and methods of using the device. The present invention also relates to a biomolecule elution strip and related methods.

Abstract: There is disclosed a method of making an array for cell assays comprising the step of providing an array of structures on a substrate, each of said structures having a pre-defined topography thereon, and wherein at least one structure has a different topography from at least one other structure.

Abstract: The present invention relates to solution microarrays. In particular, the present invention relates to an aqueous 2-phase system for solution microarrays and uses thereof. Additional embodiments are described herein.

Abstract: A method of manufacturing a substrate is provided. The method comprises, in some aspects, a) providing a support; b) forming a template by attaching a plurality of polymeric nanoparticles some or all having a core-shell structure to the support, wherein the core comprises a first polymer and the shell comprises a second polymer; and c) forming the metal nanoarray substrate by attaching a plurality of metallic nanoparticles to at least some of the polymeric nanoparticles of the template. A biosensor comprising a substrate manufactured by the method, and a method for the detection of an analyte in a sample by surface enhanced Raman spectroscopy (SERS) is also provided.

Abstract: A method of preparing a substrate with a composition comprising (i) an organoborane initiator and (ii) a radical curable component disposed thereon includes the step of depositing the composition onto the substrate wherein at least one of (i) the organoborane initiator and (ii) the radical curable component is deposited onto the substrate in the form of a gradient pattern. An article comprises the substrate and the gradient pattern formed on the substrate. The gradient pattern is formed from a developed composition comprising the reaction product of (i) the organoborane initiator and (ii) the radical curable component. By forming the gradient pattern on the substrate, combinatorial and high-throughput methods of generating and testing the developed composition are possible, which enable characterization of the developed composition for various physical and chemical properties.

Abstract: A multi-pillar structure for molecular analysis is provided. The structure comprises at least two nanopoles, each nanopole attached at one end to a substrate and freely movable along its length. The opposite ends of the at least two nanopoles are each capable of movement toward each other to trap at least one analyte molecule at their opposite ends. Each nanopole is coated with a metal coating. An array of such multi-pillar structures is also provided. A method for preparing the multi-pillar structure is further provided.

Abstract: A biologic-adsorbent, e.g., protein-adsorbent, material is prepared by forming a polymeric substrate into structures having high surface area topography whose biologic adsorbing properties can be controlled. Biologic adsorption by these structures of optimized high surface area topography is increased by mild treating of the surfaces, e.g., by oxygen plasma, without substantially altering topography. Structures can have tailored geometric features including microstructures, e.g., pillars, with a diameter from 100 nm-50 ?m and height greater than 1 ?m.

Abstract: Compounds and methods for controlling the surface properties are described. Compounds of the invention can form radicals upon exposure to irradiation, which can then react with nearby molecules to alter the surface properties of various substrates. The invention can provide surfaces that are resistant to dewetting, surfaces that have immobilized molecules such as carbohydrates and polymers immobilized, and surfaces that have metals deposited on the surface. The invention can be utilized in a wide range of application, such as sensors, microreactors, microarrays, electroless deposition of metals, and the like.

Abstract: The application discloses a process for making a polymer having pendant side groups comprising: (i) polymerising an olefinically unsaturated monomer functionalized with (a) an azide group optionally protected by a protecting group, or (b) an alkyne group optionally protected by a protecting group, by living radical polymerization, most preferably RAFT, transitional metal mediated living radical polymerization (TMM-LRP) and/or atom transfer radical polymerization, to produce a polymer intermediate; (ii) removing, when present, at least a portion of the total number of protecting groups from the polymer intermediate; (iii) reacting the polymer intermediate with at least one pendant side group moiety functionalised with (a) an alkyne group or (b) an azide group respectively so that the alkyne and azide groups react to attach the pendant side group to the polymer. Processes for making supports comprising pendant side groups, and polymers and supports prepared by the method are also disclosed.

Abstract: Magnetic nanoparticles and methods for their use in detecting biological molecules are disclosed. The magnetic nanoparticles can be attached to nucleic acid molecules, which are then captured by a complementary sequence attached to a detector, such as a spin valve detector or a magnetic tunnel junction detector. The detection of the bound magnetic nanoparticle can be achieved with high specificity and sensitivity.

Abstract: The present invention is directed to polyarylates comprising repeating units having the structure: as well as their preparation and use as cell growth substrates.

Abstract: The present invention provides synthetic membranes which are suitable as a human skin substitute for the investigation of transdermal diffusion of candidate pharmaceutical and cosmetic compounds. The membranes according to the present invention exhibit human skin-like permeability properties with respect to the diffusion of a wide range of compounds having widely different physico-chemical properties.

Abstract: Sensor array for detecting biomarkers for cancer in breath samples. The sensor array is based on 2D films or 3D assemblies of conductive nanoparticles capped with an organic coating wherein the nanoparticles are characterized by a narrow size distribution. Methods of use of the sensor array for discriminating between patterns of volatile organic compounds from healthy individuals and patients with various types of cancer are disclosed.

Abstract: A support comprising functional groups supported therein that specifically react with an aldehyde group of a sugar chain, and a polymer particle and a glycochip to which the support is applied, and uses thereof.

Abstract: Disclosed is a microanalysis chip adhesive sheet with an adhesive surface, which can be inexpensively but easily bonded to a substrate at a relatively low temperature, with which fabrication of analysis chips is simplified, and which has the capability of fixing a selective bonding substance to the sheet surface. The microanalysis chip is characterized in that a microanalysis chip adhesive sheet, which comprises a plastic sheet provided with an adhesive layer formed from an adhesive that contains a polymer in which monomer structural units that contain carboxyl radicals or acid anhydride radicals constitute 5-25% by weight of the [total] monomer structural units in the polymer, and a substrate, which has protrusions and recesses on the surface thereof, are bonded together so that the surface of the substrate having the projections and concavities and the adhesive layer of the aforementioned microanalysis chip adhesive sheet are on the inside.

Abstract: A support carrying an immobilized selective binding substance, that the support surface has a polymer containing the structural unit represented by the following General Formula (1) in an amount of 10% or more with respect all monomer units, and a selective binding substance is immobilized on the support surface by binding to the carboxyl group formed thereon via a covalent bond: (in General Formula (1), R1, R2, and R3 each represent an alkyl or aryl group or a hydrogen atom.

Abstract: An assay for the detection of antibodies to blood group antigens is provide and includes a solid substrate having immobilised thereon a fragment of cell membrane which presents a blood group antigen capable of binding to a blood group antibody. The cell fragments are preferably from red blood cells. The antigens may be immobilised in the form of an array of spots.

Abstract: A microarray of polymeric biomaterials is provided. Specifically, a microarray of polymeric biomaterials that comprises a base with a cytophobic surface, and a plurality of discrete polymeric biomaterial elements bound to the cytophobic surface, is provided. Preferably said polymeric biomaterials comprise a synthetic polymer. Said polymeric biomaterials may also comprise other compounds covalently or non-covalently attached to said synthetic polymer. Methods of preparing the microarray of polymeric biomaterials of the present invention and uses of the microarray of polymeric biomaterials of the present invention are also provided.

Abstract: Bicyclo-pyrazole compound of formula (I) and pharmaceutically acceptable salts thereof, as defined in the specification, processes for their preparation, combinatorial libraries comprising a plurality of them and pharmaceutical compositions thereof, are herewith disclosed: the compounds of the invention are useful, in therapy, as protein kinase inhibitors, for instance in the treatment of cancer.

Abstract: A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape.

Abstract: Described are devices and methods for parallel testing of formulations on various substrates.

Abstract: The present invention relates to a method of preparing a library of resins which are useful in chromatography, which method comprises creating a diversity of multi-modal ion exchange resins; and providing the diversity in a parallel system in which each resin is presented separated from the other resin(s).

Abstract: Disclosed is a thermoreversible synthesis of a new type of material made by embedding a colloidal population within a thermo-reversibly gelled polymer. The polymer may be a physically cross-linked PVA hydrogel. PVA hydrogels are non-toxic, biocompatible, mechanically robust, and elastic. The present invention TGCCA diffraction is similar to that of photo-polymerized PCCA. The present invention TGCCA can be irreversibly covalently cross-linked using glutaraldehyde. The cross-linked TGCCA can be made responsive to chemical stimuli by functionalizing the hydrogel hydroxyl groups. The diffraction of carboxyl or amine functionalized TGCCA was monitored as a function of the pH and determined diffraction wavelengths titrate with the pKa of the functional group. It was also demonstrated that TGCCA could be fabricated in arbitrarily large volumes and shapes. The present invention method fabricates inexpensive homogenously diffracting chemically modifiable TGCCA.

Abstract: In certain embodiments, this invention pertains to the creation of “Sparse Matrix” libraries of compounds. The sparse matrix libraries of this invention typically span an n-dimensional parameter (property) space at extremely sparse intervals and thereby provide a relatively small library of compounds (e.g., a library of about 200 or fewer compounds) that can be used to quickly and efficiently screen the compound parameter space for one or more desired physical, chemical, or biological properties (e.g., binding specificity, binding avidity, ?toxicity, solubility, mobility, cell permeability, serum half-life, biocompatibility, etc.).

Abstract: A block copolymer containing a) a hydrophobic biodegradable polymer, b) a hydrophilic polymer and c) at least one reactive group for covalent binding of a surface-modifying substance d) to the hydrophilic polymer b) is disclosed. Shaped bodies are formed to consist of the block copolymer and are utilized as carriers for tissue culture and active substances and for controlled release and targeted administration of active substances.

Abstract: A functional group and spacer group modified polymer composition, articles incorporating the composition, and methods for label-independent-detection using the articles, as defined herein.

Abstract: The present invention provides diverse chemical libraries bound to small particle with paramagnetic properties. Typically, the chemical structures comprise a plurality of different chemical moieties, the particles are paramagnetic and have a diameter between about 100 nm and about 10 microns, the chemical structures bound to each particular particle have substantially the same structure and the combinatorial library comprises at least 100,000 different chemical structures.

Abstract: Bicyclo-pyrazole compound of formula (I) and pharmaceutically acceptable salts thereof, as defined in the specification, processes for their preparation, combinatorial libraries comprising a plurality of them and pharmaceutical compositions thereof, are herewith disclosed: the compounds of the invention are useful, in therapy, as protein kinase inhibitors, for instance in the treatment of cancer.

Abstract: The invention relates to processes and devices for the controlled fabrication of nanostructures from starting components that have high fidelity recognition properties and multiple binding groups. In one embodiment, the invention relates to the formation of nanostructures using controlled sequential addition of nanocomponents at regular intervials via sequential formation of binding pairs or other chemical binding reactions.

Abstract: Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 ?m2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.

Abstract: The present invention provides crosslinked epoxy-functional copolymer films and microarrays built from the crosslinked epoxy-functional copolymer films. Microarrays incorporating the copolymers include a substrate on which a film of the crosslinked epoxy-functional copolymer is disposed and target molecules bound to the copolymer film. The crosslinked polymer films are well-suited for use as scaffolds for target molecules in microarrays because they provide a high density of binding sites for the target molecules, are mechanically stable, and may be coated onto a wide range of substrates.

Abstract: A method of preparing a substrate with a composition comprising (i) an organoborane initiator and (ii) a radical curable component disposed thereon includes the step of depositing the composition onto the substrate wherein at least one of (i) the organoborane initiator and (ii) the radical curable component is deposited onto the substrate in the form of a gradient pattern. An article comprises the substrate and the gradient pattern formed on the substrate. The gradient pattern is formed from a developed composition comprising the reaction product of (i) the organoborane initiator and (ii) the radical curable component. By forming the gradient pattern on the substrate, combinatorial and high-throughput methods of generating and testing the developed composition are possible, which enable characterization of the developed composition for various physical and chemical properties.

Abstract: A sensor that includes a) a silicon (Si) substrate having a surface; and b) a monolayer of oligoethylene glycol (OEG) bonded to the surface via silicon-carbon bonds. Regions of the OEG monolayer distal to the surface are functionalized with a ligand serving as a recognition element for a bioanalyte. The ligand is covalently bonded in these regions as a cycloadduct of a 1,3-dipolar cycloaddition reaction. A method of making a silicon surface that recognizes a biological specimen includes 1) hydrosilylating with a mixture that includes an oligoethylene glycol (OEG) substituted with an alkene at one end of the OEG and capped at the opposing end of the OEG and an oligoethylene glycol (OEG) substituted with an alkene at one end of the OEG and an alkyne having a protecting group at the opposing end of the OEG and 2) removing the protecting group from the alkyne; and 3) reacting the alkyne with a reagent in a 1,3-dipolar cycloaddition.

Abstract: The application discloses a process for making a polymer having pendant side groups comprising: (i) polymerising an olefinically unsaturated monomer functionalised with (a) an azide group optionally protected by a protecting group, or (b) an alkyne group optionally protected by a protecting group, by living radical polymerisation, most preferably RAFT, transitional metal mediated living radical polymerisation (TMM-LRP) and/or atom transfer radical polymerisation, to produce a polymer intermediate; (ii) removing, when present, at least a portion of the total number of protecting groups from the polymer intermediate; (iii) reacting the polymer intermediate with at least one pendant side group moiety functionalised with (a) an alkyne group or (b) an azide group respectively so that the alkyne and azide groups react to attach the pendant side group to the polymer. Processes for making supports comprising pendant side groups, and polymers and supports prepared by the method are also disclosed.

Abstract: A biosensor chip includes a substrate, a polymer having an anionic functional group and being arranged on a surface of the substrate, a polyamino group which is directly or indirectly bound to the anionic functional group at a surface of the polymer, and a long-chain alkyl-based group which is directly or indirectly bound to the anionic functional group at the surface of the polymer.

Abstract: An object of the present invention is to provide a probe array having partitioned array regions with uniform surface chemical properties. The probe array of the present invention includes a substrate having a plurality of partitioned array regions where many probes are immobilized and a separator attached to the substrate and including partitions partitioning the array regions. The above object can be achieved by attaching the separator including the partitions capable of partitioning instead of forming hydrophobic regions on a surface of the substrate by printing or chemical treatment.

Abstract: A microarray has a substrate and a plurality of three-dimensional microstructures formed on the substrate. Each of the three-dimensional microstructures is made with polymer material and has a plurality of reactive sites formed on its surface and interior pores. The polymer material is polymer gel or other porous polymer. The combination of three-dimensional microstructure and porous polymer material increases the surface area of the microstructure and density of the reactive sites on the surface of the microstructures. The higher density of reactive sites increases the luminescence, visibility or instrument detectability of the interaction between analytes and reactive microstructure sites on the microarray. A plurality of chemical groups are respectively attached to the reactive sites. The chemical groups each include at least one monomer. The chemical groups may have different chemical structures. A plurality of microchannels can be formed around the microstructures for isolation.