Immunological Separation Or Affinity Chromatography Patents (Class 530/413)
  • Patent number: 10519194
    Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal, of protein, fragments without adversely affecting the yield of the desired protein product.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: December 31, 2019
    Assignee: Merck Patent GmbH
    Inventors: Mikhail Kozlov, Matthew T. Stone, Romas Skudas, Kevin Galipeau
  • Patent number: 10494429
    Abstract: Purified recombinant polypeptides isolated from Chinese hamster ovary host cells, including antibodies, such as therapeutic antibodies, and methods of making and using such polypeptides are provided.
    Type: Grant
    Filed: February 22, 2018
    Date of Patent: December 3, 2019
    Assignee: GENENTECH, INC.
    Inventors: X. Christopher Yu, Atia Naim, Christopher A. Teske, Martin Vanderlaan
  • Patent number: 9737829
    Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example after a flow-through collection of a target and prior to cleaning the chromatography solid support.
    Type: Grant
    Filed: February 14, 2017
    Date of Patent: August 22, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Larry J. Cummings
  • Patent number: 9545627
    Abstract: A method for producing a protein adsorbent comprising a substrate and a molecular chain fixed on the surface of the substrate is disclosed. The method comprises, in this order: a dry-heat treatment step of heating a pretreatment adsorbent comprising the substrate and the molecular chain fixed on the surface of the substrate, in which the molecular chain contains a weak electrolytic ion-exchange group; and a wet-heat treatment step of heating the pretreatment adsorbent in a moistened state with a liquid or steam to obtain the protein adsorbent.
    Type: Grant
    Filed: December 12, 2012
    Date of Patent: January 17, 2017
    Assignee: ASAHI KASEI CHEMICALS CORPORATION
    Inventors: Naoyuki Shinohara, Yuta Sato
  • Patent number: 9458425
    Abstract: A process for obtaining human derived embryoid bodies (hEB). Human embryonic stem cells are incubated in vitro in a liquid growth medium under conditions in which the cells undergo differentiation, but do not adhere to the walls of the container. The invention also provides hEBs obtained by the process.
    Type: Grant
    Filed: May 12, 2000
    Date of Patent: October 4, 2016
    Assignees: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD., YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM
    Inventors: Joseph Itskovitz-Eldor, Nissim Benvenisty
  • Patent number: 9441011
    Abstract: A method is disclosed for purifying an antibody monomer, comprising providing a porous membrane comprising a hydrophobic porous substrate, a hydrophilic molecular chain of a different material from that of the porous substrate, immobilized on the surface of pores of the porous substrate, and a side chain of the molecular chain, containing a nitrogen atom to which one to three alkyl groups each having two or three carbon atoms are bonded; passing an antibody solution containing antibody aggregates of dimers or higher-order multimers through the porous membrane to adsorb the antibody aggregates to the porous membrane; and recovering the purified antibody monomer in the passed solution.
    Type: Grant
    Filed: June 29, 2010
    Date of Patent: September 13, 2016
    Assignees: ASAHI KASEI CHEMICALS CORPORATION, ASAHI KASEI MEDICAL CO., LTD.
    Inventors: Naoyuki Shinohara, Takashi Ishihara, Hironobu Shirataki, Yoshiro Yokoyama
  • Patent number: 9127043
    Abstract: The present invention pertains to improved methods of using dye-ligand affinity chromatography for the isolation of antibodies or proteins comprising an antibody fragment (such as Fc fusion proteins) from a mixture of undesirable contaminants. In particular, the use of an organic polymer such as polyethylene glycol (PEG) in the elution phase of an antibody/dye-ligand chromatography isolation procedure results in improved separation of target antibodies from undesirable contaminants. The methods described herein are particularly useful in separating or removing antibody aggregates, misfolded antibodies, and virus contaminants from target antibodies.
    Type: Grant
    Filed: March 4, 2010
    Date of Patent: September 8, 2015
    Assignee: BIOGEN MA INC.
    Inventors: Robert S. Gronke, Héctor Zaanoni
  • Publication number: 20150133618
    Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2?CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
    Type: Application
    Filed: April 22, 2013
    Publication date: May 14, 2015
    Inventors: Jesper Hanssen, Gustav Rodrigo, Tobias E Soderman
  • Patent number: 9023994
    Abstract: The invention relates to an immunoglobulin composition reduced in thrombogenic agents and to methods for its preparation. One method comprises subjecting an immunoglobulin containing solution to a negative cation exchanger chromatography at a pH in the range of higher than 3.8 to equal to or lower than 5.3. The solution can also be subjected to a negative anion exchanger chromatography at a pH in the range of 7 to 8.2.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: May 5, 2015
    Assignee: Omrix Biopharmaceuticals, Ltd.
    Inventors: Roni Mintz, Oleg Belyaev, Israel Nur, Liliana Bar, Roberto Meidler
  • Patent number: 9024000
    Abstract: The present invention relates to a method to improve the sorbent efficiency of protein A chromatography using switching column with continuous feeding. In the chromatography method of the present invention, the increased usage efficiency of the absorbent (resin), the decreased processing time, the decreased operation cycle of column compared to that of single batch-type column, and the reduced amount of used resin are achieved and thus, the target protein can be purified at a high efficiency and a low cost.
    Type: Grant
    Filed: April 26, 2011
    Date of Patent: May 5, 2015
    Assignee: Celltrion, Inc.
    Inventors: Su Hi Jeon, Jin-Il Kim
  • Patent number: 9018361
    Abstract: Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: April 28, 2015
    Assignee: AbbVie Inc.
    Inventors: Robert K. Hickman, Qing Huang, Cheryl L. Weed, Scott T. Ennis, Barbara Perilli-Palmer, Min Wan
  • Patent number: 9012212
    Abstract: A method and system is provided for yielding biopharmaceutical products involving a chromatographic separation process. The method comprises: providing a plurality of membrane adsorber cartridges; providing a plurality of valves, communicatively coupled to said plurality of membrane adsorber cartridges; and switching the valves, so as to interconnect said membrane adsorber cartridges to operate in a countercurrent flow mode. The system comprises multiple membrane adsorber cartridges that are interconnected and configured to operate in a countercurrent flow mode. Furthermore, the configuration comprises a valve assembly that allows the cartridges to be subjected to different steps in the process by automatic switching of the valves. In this way, cartridges are recycled many times during the purification of a batch.
    Type: Grant
    Filed: April 17, 2007
    Date of Patent: April 21, 2015
    Assignee: Xendo Holding B.V.
    Inventors: Marc Antonius Theodorus Bisschops, Jozef Anton Mari Pennings, Jacob Arthur Tijsterman
  • Publication number: 20150093800
    Abstract: The present invention provides methods for cleaning or regenerating a chromatography materiel for reuse. The methods of the invention can be used for cleaning or regenerating chromatography columns for reuse in the large-scale manufacture of multiple polypeptide products.
    Type: Application
    Filed: September 5, 2014
    Publication date: April 2, 2015
    Inventors: Ekta MAHAJAN, Kapil KOTHARY, Joanna SO, Jay WERBER
  • Patent number: 8993519
    Abstract: Described herein are compositions and methods for treating, preventing and ameliorating diseases and conditions characterized by a lower than normal white blood cell count, such as leukopenia and neutropenia. The compositions and methods include recombinant human albumin-human granulocyte colony stimulating factor. Pharmaceutical formulations including the recombinant fusion protein, and methods of making such formulations are also described.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: March 31, 2015
    Assignee: Teva Pharmaceutical Industries Ltd.
    Inventors: Jason Benjamin Bock, Xia Luo
  • Publication number: 20150087532
    Abstract: Disclosed herein are methods and compositions for purifying proteins from crude solutions.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 26, 2015
    Inventors: Patrick Brown, R. Edward Watts, Pehr Harbury
  • Patent number: 8975370
    Abstract: The present invention relates to a chimeric inhibitor protein of a protease comprising an inhibiting polypeptidic sequence and at least one polypeptidic sequence of a substrate-enzyme interaction site specific for a protease. Other objects of the invention are to provide a purified and isolated DNA sequence encoding the chimeric inhibitor protein of a protease, an expression vector characterized in that it comprises said purified and isolated DNA sequence, a eukaryotic or prokaryotic host cell transformed with this expression vector and a method of producing a chimeric inhibitor protein.
    Type: Grant
    Filed: April 5, 2004
    Date of Patent: March 10, 2015
    Assignee: Universite de Lausanne
    Inventors: David Deperthes, Sylvain Cloutier
  • Patent number: 8975384
    Abstract: The present invention provides a tag peptide comprising an amino acid sequence represented by the following formula (I): X1-Tyr-X2-Gly-Gln-X3??(I) (wherein X1, X2 and X3 are the same or different and each represent any amino acid residue) and an antibody against the tag peptide. By combined use of the tag peptide and antibody of the present invention, a system that enables proteins expressed from cloned genes to be highly purified in an inexpensive and easy manner can be established.
    Type: Grant
    Filed: December 18, 2008
    Date of Patent: March 10, 2015
    Assignee: Osaka University
    Inventor: Junichi Takagi
  • Publication number: 20150065696
    Abstract: The invention is directed to an apparatus and method for purifying a protein. The apparatus involves the use of a capture chromatography resin, a depth filter arranged after the capture chromatography resin, and a mixed-mode chromatography resin arranged after the depth filter. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, a depth filter, and a mixed-mode chromatography resin. A membrane adsorber or monolith may be substituted for the mixed-mode chromatography column.
    Type: Application
    Filed: June 4, 2014
    Publication date: March 5, 2015
    Inventors: Chen Wang, Robert K. Hickman, I, Edwin O. Lundell, Roy D. Hegedus
  • Publication number: 20150064192
    Abstract: Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.
    Type: Application
    Filed: November 14, 2014
    Publication date: March 5, 2015
    Inventors: DANIEL M. GORMAN, TROY D. RANDALL, ALBERT ZLOTNIK
  • Patent number: 8962813
    Abstract: A process for manufacturing of a composition containing a purified factor for supporting wound healing selected from the group consisting of Hepatocyte Growth Factor (HGF) platelet derived growth factor (PDGF), Epidermal growth factor (EGF), transforming growth factor alfa (TGF-?), Transforming growth factor beta (TGF-?), insulin like growth factor (IGF-I) and Fibroblast growth factor (FGF) from sources, such as blood, containing the factor for supporting wound healing, wherein the manufacturing process comprises purification steps which are performed in the presence of antithrombin III (AT-III).
    Type: Grant
    Filed: January 25, 2007
    Date of Patent: February 24, 2015
    Assignee: Octapharma AG
    Inventors: Andrea Neisser-Svae, Stefan Winge, Anna Mjärdestam
  • Patent number: 8962814
    Abstract: Fully human monoclonal Abs includes (i) an antigen-binding variable region that exhibits very high binding affinity for IL-1? and (ii) a constant region that is effective at both activating the complement system though C1q binding and binding to several different Fc receptors.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: February 24, 2015
    Assignee: XBiotech, Inc.
    Inventor: John Simard
  • Patent number: 8952138
    Abstract: A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: February 10, 2015
    Assignee: Amgen Inc.
    Inventors: Joseph Edward Shultz, Roger Hart, Ronald Nixon Keener, III
  • Patent number: 8945872
    Abstract: Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for production and high expression of rhGDF-5 protein are disclosed herein. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality.
    Type: Grant
    Filed: January 25, 2013
    Date of Patent: February 3, 2015
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: David S. Scher, Roger E. Harrington
  • Publication number: 20150030661
    Abstract: The invention provides, inter alia, multilayer films of alternating layers of a glycosylated polymer (e.g. a mucin) and a lectin, as well as methods of making and using these films. The films can be adapted for, inter alia, delivery of a biologically active agent providing a non-toxic substrate to support cell growth, replication, and/or maintenance as well as detecting a microorganism and/or reducing microorganism adherence to a surface.
    Type: Application
    Filed: February 6, 2013
    Publication date: January 29, 2015
    Inventors: Katharina Ribbeck, Thomas Crouzier
  • Patent number: 8940878
    Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: January 27, 2015
    Assignee: Amgen Inc.
    Inventors: Joseph Edward Shultz, Roger Hart
  • Patent number: 8933206
    Abstract: The present invention provides a new method for isolation and/or purification of immunoglobulins from a solution containing one or more immunoglobulins using a solid phase matrix represented by the formula: M-SP-L, wherein M is designates a matrix backbone, SP designates a spacer and L designates a substituted benzimidazole ligand.
    Type: Grant
    Filed: January 8, 2008
    Date of Patent: January 13, 2015
    Assignee: Millipore (U.K.) Limited
    Inventors: Gordon R. Duffin, Victoria Jane Dolan, Katherine Louise Angus, Andrew Lyddiatt
  • Patent number: 8933205
    Abstract: The invention provides a process for preparing a cell-binding agent chemically coupled to a drug. The process comprises covalently attaching a linker to a cell-binding agent, a purification step, conjugating a drug to the cell-binding agent and a subsequent purification step.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: January 13, 2015
    Assignee: ImmunoGen, Inc.
    Inventors: Yong Dai, Yong Wang, Shengjin Jin, Deborah H. Meshulam, Godfrey W. Amphlett
  • Publication number: 20150005480
    Abstract: The present invention pertains to an affinity tag system for the immobilisation and/or purification of molecules such as biological or organic molecules. The invention provides EF-hand subdomains of calcium binding proteins, such as calbindin D9k, as affinity tags and affinity ligands for immobilising, detecting and/or for purifying molecules, particularly proteins. Also provided are methods utilising the affinity tag system of the invention, affinity matrices comprising EF-hand subdomain affinity ligands and fusion proteins comprising EF-hand subdomain affinity tags.
    Type: Application
    Filed: January 7, 2013
    Publication date: January 1, 2015
    Applicant: UNIVERSITY COLLEGE DUBLIN
    Inventors: David O'Connell, Sara Linse, Eva Thulin, Alejandro Merino
  • Patent number: 8921531
    Abstract: Protein A crystals and Protein A cross-linked protein crystals (CLPCs) are described. Methods of preparing and using are also disclosed.
    Type: Grant
    Filed: September 13, 2010
    Date of Patent: December 30, 2014
    Assignee: Althea Technologies, Inc.
    Inventors: Bhami Shenoy, Sibyl Baladi, Reena Patel, Margaret McGrath, Nazer Khalaf, Chanchal Randhawa
  • Patent number: 8916387
    Abstract: Methods are provided for the prevention, treatment and diagnosis of Alzheimer's disease, based on the glycosylation pattern of amyloid-beta peptides in body fluids and tissues.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: December 23, 2014
    Inventors: Jonas Nilsson, Adnan Halim, Göran Larson, Kaj Blennow, Gunnar Brinkmalm, Erik Portelius, Henrik Zetterberg
  • Patent number: 8916691
    Abstract: Methods of expressing LIM mineralization protein in mammalian cells are described. Methods of expressing LIM mineralization protein and assessing glycosylation of the LIM mineralization protein in prokaryotic and non-mammalian eukaryotic cells are also described. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein. Transfection may be accomplished in vitro, ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid.
    Type: Grant
    Filed: October 10, 2006
    Date of Patent: December 23, 2014
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: Scott D. Boden, Sreedhara Sangadala, F. Louisa Titus, William F. McKay
  • Patent number: 8911992
    Abstract: Removal of genomic DNA from biological samples using cation exchange is described.
    Type: Grant
    Filed: April 28, 2011
    Date of Patent: December 16, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Patent number: 8912117
    Abstract: The present invention provides a chromatography packing material having improved flow rate characteristics and adsorption characteristics. In particular, the present invention provides a chromatography packing material suitable for separation and purification of immunoglobulin in the manufacture of antibody preparations. A porous cellulose gel, which is made by adding polysaccharides having a limiting viscosity of 0.21 to 0.90 dL/g to porous cellulose particles, the dry weight per unit volume of the porous cellulose gel being 1.06 to 1.40 times the dry weight per unit volume of the porous cellulose particles, is used. By adding a predetermined amount of polysaccharides having a predetermined limiting viscosity to porous cellulose particles, flow rate characteristics and adsorption characteristics can be improved.
    Type: Grant
    Filed: February 18, 2010
    Date of Patent: December 16, 2014
    Assignee: JNC Corporation
    Inventors: Yoshihiro Matsumoto, Yasuto Umeda, Shigeyuki Aoyama, Masami Todokoro
  • Publication number: 20140348845
    Abstract: Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane,
    Type: Application
    Filed: December 18, 2012
    Publication date: November 27, 2014
    Inventors: Jerome Joseph Bill, Jr., Arick Michael Brown, Christopher John Dowd, Brooke Ellen Thayer
  • Patent number: 8895709
    Abstract: Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
    Type: Grant
    Filed: October 20, 2009
    Date of Patent: November 25, 2014
    Assignee: AbbVie Inc.
    Inventors: Robert K. Hickman, Qing Huang, Cheryl L. Weed, Scott T. Ennis, Barbara Perilli-Palmer, Min Wan
  • Patent number: 8895710
    Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
    Type: Grant
    Filed: January 4, 2011
    Date of Patent: November 25, 2014
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Carina Engstrand, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J. Johansson, Jean-Luc Maloisel
  • Patent number: 8889837
    Abstract: The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
    Type: Grant
    Filed: January 24, 2011
    Date of Patent: November 18, 2014
    Assignee: Hoffman-La Roche Inc.
    Inventors: Josef Burg, Klaus Reichert, Axel Schroth, Hartmut Schurig, Axel Wessner
  • Patent number: 8889376
    Abstract: A fusion protein that includes a polypeptide binding specifically to a constant region of an antibody and a stabilization protein linked to a terminus of the polypeptide, a polynucleotide encoding the fusion protein, a cell including the polynucleotide, a method of preparing the fusion protein, and a method of isolating an antibody by using the fusion protein.
    Type: Grant
    Filed: August 6, 2010
    Date of Patent: November 18, 2014
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Jae-Il Lee, Young-Sun Lee, Tae-Soo Lee
  • Patent number: 8889423
    Abstract: A method for analyzing a binding ability of protein to a compound, comprising the steps of (a) fractionating a first group of isotope-labeled proteins into plural fractions using a carrier having the compound immobilized thereon; (b) fractionating a second group of proteins into one or plural fractions using a carrier having the compound immobilized thereon; (c) adding an amount of at least one fraction obtained in step (b) to each of the fractions obtained in step (a); (d) analyzing the fractions obtained in step (c) with mass spectrometry; and (e) based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between a peak derived from a protein in the fraction obtained in step (a) and a peak derived from a protein in the fraction obtained in step (b), and comparing degrees of the binding ability of the plural kinds of proteins to the compound.
    Type: Grant
    Filed: July 31, 2006
    Date of Patent: November 18, 2014
    Assignee: Eisai R & D Management Co., Ltd.
    Inventors: Yoshiya Oda, Hiroyuki Katayama
  • Publication number: 20140329706
    Abstract: Affinity tags and ligands comprising a PDZ domain peptide and a PDZ binding carboxy terminal peptide and related engineered protein, engineered labels, compositions, methods and systems.
    Type: Application
    Filed: May 1, 2014
    Publication date: November 6, 2014
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventor: Ward Gale WALKUP, IV
  • Publication number: 20140316108
    Abstract: A coating of a random copolymer of acrylamide and a second monomer, e.g. glycidoxylmethacrylate, for a silica surface is described. The coating is applied to chromatographic support structures having silica based surfaces. The coating is functionalized to produce protein chromatography matrices that are particularly useful for extracting trace amounts of biomarker molecules from biological samples.
    Type: Application
    Filed: November 1, 2012
    Publication date: October 23, 2014
    Inventors: Mary J. Wirth, Yirmin Hua, Zhaorui Zhang
  • Publication number: 20140309408
    Abstract: The invention relates to a method for purifying compositions containing virus-like particles (VLP), wherein a VLP-containing composition is filtered through a filter medium, in particular through a membrane, having a molecular weight cut off (MWCO) of more than 30 kDa, and the cell culture supernatant of VLP-expressing cells is used as a VLP-containing composition. The invention further relates to a VLP-containing composition which can be produced by this method.
    Type: Application
    Filed: August 1, 2012
    Publication date: October 16, 2014
    Applicant: LIFE SCIENCE INKUBATOR BETRIEBS GMBH & CO. KG
    Inventors: Victoria Demina, Heiko Manninga
  • Publication number: 20140301977
    Abstract: The present invention provides methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant by overloading a chromatography material and eluting the product.
    Type: Application
    Filed: November 2, 2012
    Publication date: October 9, 2014
    Inventors: Deepa Nadarajah, Amit Mehta
  • Patent number: 8852435
    Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.
    Type: Grant
    Filed: November 29, 2011
    Date of Patent: October 7, 2014
    Assignee: Therapeutics Proteins International, LLC
    Inventor: Sarfaraz Niazi
  • Patent number: 8853371
    Abstract: Methods useful for producing a solution of purified Fc peptide chains are disclosed. The methods employ protein A chromatography to separate high molecular weight, aggregated peptide chains comprising antibody Fc domains from lower molecular weight unaggregated peptide chains that also comprise antibody Fc domains. The solutions of purified Fc peptide chains obtained by the methods of the invention contain less than 5% aggregate and greater than 70% of the Fc peptide chains subjected to purification.
    Type: Grant
    Filed: October 19, 2007
    Date of Patent: October 7, 2014
    Assignee: Janssen Biotech, Inc.
    Inventors: Pedro Alfonso, Michael Capaldi, Dennis Dong, Thomas R. Gervais, Joshua Goldstein, Allen B. Magill, Nicole Quinlan, Deepak Saini
  • Patent number: 8846877
    Abstract: An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.
    Type: Grant
    Filed: September 24, 2009
    Date of Patent: September 30, 2014
    Assignee: JSR Corporation
    Inventors: Kouji Tamori, Tetsuo Fukuta, Masaaki Miyaji, Yong Wang, Takayoshi Abe, Yuusuke Okano, Masaki Momiyama, Takahiro Kawai
  • Patent number: 8846032
    Abstract: The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.
    Type: Grant
    Filed: August 27, 2012
    Date of Patent: September 30, 2014
    Assignee: MedImmune, LLC
    Inventors: Jonathan Liu, Richard Schwartz, Mark Thompson, Luis Maranga, Mridul Ghosh, Ajit Subramanian, Simon Sheng-Tsiung Hsu
  • Publication number: 20140256919
    Abstract: A device for carrying out chemical and/or biochemical processes comprises at least two bodies which are arranged axially on top of one another and have in each case at least one cavity. The bodies are rotatable against one another on the basis of a centrifugal force or a similarly acting force. At least one cavity is provided with a membrane, the permeability of which to liquids is dependent on the acting centrifugal force or the similarly acting force.
    Type: Application
    Filed: March 3, 2014
    Publication date: September 11, 2014
    Applicant: ROBERT BOSCH GMBH
    Inventors: Arne Kloke, Juergen Steigert
  • Patent number: 8822640
    Abstract: The present invention relates to a new streptavidin muteins. This mutein is The muteins are capable of oligomerization to form tetramers, with relatively strong subunit interactions, dissociation constant (KD) for biotin in this mutein in the range of 10?7 to 10?8M, off-rate (koff) for the bound biotin in the streptavidin-biotin complex in the range of 10?4 sec?1, stable enough to allow reuse, and producible producable with reasonable production yield via secretion in a soluble functional state without the requirement of refolding streptavidin via the tedious and expensive denaturation and renaturation processes.
    Type: Grant
    Filed: October 13, 2011
    Date of Patent: September 2, 2014
    Assignee: UTI Limited Partnership
    Inventors: Sui-Lam Wong, Sau-Ching Wu, Isabelle Barrette-Ng, Ken Ng
  • Publication number: 20140228548
    Abstract: The invention provides methods for separating a polypeptide of interest (such as an antibody) from a virus. In some embodiments, the methods involve eluting the polypeptide of interest from a Protein A resin with an elution buffer have a particular range of conductivity values that minimizes the amount of virus that co-elutes with the polypeptide of interest.
    Type: Application
    Filed: August 31, 2012
    Publication date: August 14, 2014
    Applicant: Human Genome Sciences
    Inventor: Olga Galperina