Immunological Separation Or Affinity Chromatography Patents (Class 530/413)
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Patent number: 8889837Abstract: The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.Type: GrantFiled: January 24, 2011Date of Patent: November 18, 2014Assignee: Hoffman-La Roche Inc.Inventors: Josef Burg, Klaus Reichert, Axel Schroth, Hartmut Schurig, Axel Wessner
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Patent number: 8889376Abstract: A fusion protein that includes a polypeptide binding specifically to a constant region of an antibody and a stabilization protein linked to a terminus of the polypeptide, a polynucleotide encoding the fusion protein, a cell including the polynucleotide, a method of preparing the fusion protein, and a method of isolating an antibody by using the fusion protein.Type: GrantFiled: August 6, 2010Date of Patent: November 18, 2014Assignee: Samsung Electronics Co., Ltd.Inventors: Jae-Il Lee, Young-Sun Lee, Tae-Soo Lee
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Publication number: 20140329706Abstract: Affinity tags and ligands comprising a PDZ domain peptide and a PDZ binding carboxy terminal peptide and related engineered protein, engineered labels, compositions, methods and systems.Type: ApplicationFiled: May 1, 2014Publication date: November 6, 2014Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventor: Ward Gale WALKUP, IV
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Publication number: 20140316108Abstract: A coating of a random copolymer of acrylamide and a second monomer, e.g. glycidoxylmethacrylate, for a silica surface is described. The coating is applied to chromatographic support structures having silica based surfaces. The coating is functionalized to produce protein chromatography matrices that are particularly useful for extracting trace amounts of biomarker molecules from biological samples.Type: ApplicationFiled: November 1, 2012Publication date: October 23, 2014Inventors: Mary J. Wirth, Yirmin Hua, Zhaorui Zhang
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Publication number: 20140309408Abstract: The invention relates to a method for purifying compositions containing virus-like particles (VLP), wherein a VLP-containing composition is filtered through a filter medium, in particular through a membrane, having a molecular weight cut off (MWCO) of more than 30 kDa, and the cell culture supernatant of VLP-expressing cells is used as a VLP-containing composition. The invention further relates to a VLP-containing composition which can be produced by this method.Type: ApplicationFiled: August 1, 2012Publication date: October 16, 2014Applicant: LIFE SCIENCE INKUBATOR BETRIEBS GMBH & CO. KGInventors: Victoria Demina, Heiko Manninga
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Publication number: 20140301977Abstract: The present invention provides methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant by overloading a chromatography material and eluting the product.Type: ApplicationFiled: November 2, 2012Publication date: October 9, 2014Inventors: Deepa Nadarajah, Amit Mehta
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Patent number: 8852435Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.Type: GrantFiled: November 29, 2011Date of Patent: October 7, 2014Assignee: Therapeutics Proteins International, LLCInventor: Sarfaraz Niazi
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Patent number: 8853371Abstract: Methods useful for producing a solution of purified Fc peptide chains are disclosed. The methods employ protein A chromatography to separate high molecular weight, aggregated peptide chains comprising antibody Fc domains from lower molecular weight unaggregated peptide chains that also comprise antibody Fc domains. The solutions of purified Fc peptide chains obtained by the methods of the invention contain less than 5% aggregate and greater than 70% of the Fc peptide chains subjected to purification.Type: GrantFiled: October 19, 2007Date of Patent: October 7, 2014Assignee: Janssen Biotech, Inc.Inventors: Pedro Alfonso, Michael Capaldi, Dennis Dong, Thomas R. Gervais, Joshua Goldstein, Allen B. Magill, Nicole Quinlan, Deepak Saini
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Patent number: 8846877Abstract: An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.Type: GrantFiled: September 24, 2009Date of Patent: September 30, 2014Assignee: JSR CorporationInventors: Kouji Tamori, Tetsuo Fukuta, Masaaki Miyaji, Yong Wang, Takayoshi Abe, Yuusuke Okano, Masaki Momiyama, Takahiro Kawai
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Patent number: 8846032Abstract: The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.Type: GrantFiled: August 27, 2012Date of Patent: September 30, 2014Assignee: MedImmune, LLCInventors: Jonathan Liu, Richard Schwartz, Mark Thompson, Luis Maranga, Mridul Ghosh, Ajit Subramanian, Simon Sheng-Tsiung Hsu
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Publication number: 20140256919Abstract: A device for carrying out chemical and/or biochemical processes comprises at least two bodies which are arranged axially on top of one another and have in each case at least one cavity. The bodies are rotatable against one another on the basis of a centrifugal force or a similarly acting force. At least one cavity is provided with a membrane, the permeability of which to liquids is dependent on the acting centrifugal force or the similarly acting force.Type: ApplicationFiled: March 3, 2014Publication date: September 11, 2014Applicant: ROBERT BOSCH GMBHInventors: Arne Kloke, Juergen Steigert
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Patent number: 8822640Abstract: The present invention relates to a new streptavidin muteins. This mutein is The muteins are capable of oligomerization to form tetramers, with relatively strong subunit interactions, dissociation constant (KD) for biotin in this mutein in the range of 10?7 to 10?8M, off-rate (koff) for the bound biotin in the streptavidin-biotin complex in the range of 10?4 sec?1, stable enough to allow reuse, and producible producable with reasonable production yield via secretion in a soluble functional state without the requirement of refolding streptavidin via the tedious and expensive denaturation and renaturation processes.Type: GrantFiled: October 13, 2011Date of Patent: September 2, 2014Assignee: UTI Limited PartnershipInventors: Sui-Lam Wong, Sau-Ching Wu, Isabelle Barrette-Ng, Ken Ng
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Publication number: 20140228548Abstract: The invention provides methods for separating a polypeptide of interest (such as an antibody) from a virus. In some embodiments, the methods involve eluting the polypeptide of interest from a Protein A resin with an elution buffer have a particular range of conductivity values that minimizes the amount of virus that co-elutes with the polypeptide of interest.Type: ApplicationFiled: August 31, 2012Publication date: August 14, 2014Applicant: Human Genome SciencesInventor: Olga Galperina
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Patent number: 8802828Abstract: Methods for refolding antibodies, particularly monoclonal antibodies, from aggregated and/or denatured preparations by subjecting the antibody preparation to high hydrostatic pressure are provided. Refolded preparations of antibodies produced by the methods described herein are also provided.Type: GrantFiled: February 27, 2012Date of Patent: August 12, 2014Assignee: Barofold, Inc.Inventors: Matthew B. Seefeldt, Robert Nelson, Theodore W. Randolph, Lyndal K. Hesterberg
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Publication number: 20140220633Abstract: The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second cType: ApplicationFiled: April 7, 2014Publication date: August 7, 2014Applicant: HOFFMANN-LA ROCHE INC.Inventors: Roberto Falkenstein, Birgit Weydanz, Nicole Fuehrler, Claudia Giessel, Sybille Gabel, Adelbert Grossmann, Friederike Hesse, Marc Pompiati, Andreas Schaubmar, Brigitte Kraemer
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Publication number: 20140220591Abstract: There is described a method for analyzing ubiquitin polymers using linkage-specific deubiquitinase enzymes. Novel specificities of deubiquitinase enzymes are also provided.Type: ApplicationFiled: September 24, 2012Publication date: August 7, 2014Applicant: Medical Research CouncilInventors: Tycho E.T. Mevissen, Manuela K. Hospenthal, David Komander
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Patent number: 8796431Abstract: The present invention relates to processes for the production of peptides, and the peptides produced accordingly. Peptides produced according to the invention may be produced more efficiently than peptides produced according to prior art processes. The production process of the invention may lead to advantages in yield, purity, and/or price. Methods of marketing peptides are also disclosed.Type: GrantFiled: November 9, 2010Date of Patent: August 5, 2014Assignees: The Regents of the University of Colorado, a body corporate, AmideBio LLCInventors: Michael H. B. Stowell, Jonathan Caruthers, Travis Nemkov, Brian Hiester, Leslie Boux, Mikhail Plam
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Patent number: 8779110Abstract: The invention provides an efficient method of purification of a modified cytokine. The process includes the use of a chromatographic technique for the purification of the desired cytokine. The purified cytokine can be used as a therapeutic composition.Type: GrantFiled: June 23, 2009Date of Patent: July 15, 2014Assignees: Dr. Reddy's Laboratories Limited, Dr. Reddy's Laboratories, Inc.Inventors: Chaiti Roy, Darshan Koticha, Vivek Arthanari
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Patent number: 8772018Abstract: The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.Type: GrantFiled: July 7, 2008Date of Patent: July 8, 2014Assignee: EMD Millipore CorporationInventors: Nanying Bian, Senthil Ramaswamy, Neil Soice, Chen Wang, Yuan Wong
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Publication number: 20140171626Abstract: Processes for the purification of NY-ESO-1 related polypeptides, as well as polypeptides produced by these processes are provided herein; a composition comprising pure and stable NY-ESO-1 is also provided.Type: ApplicationFiled: March 8, 2013Publication date: June 19, 2014Applicant: GlaxoSmithKline LLCInventors: Louis Patrick GAGNON, John Williams
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Patent number: 8754196Abstract: The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as, Staphylococcus aureus Protein A (SpA), as well as methods of using the same.Type: GrantFiled: June 6, 2012Date of Patent: June 17, 2014Assignee: EMD Millipore CorporationInventors: Shari Spector, Robert Smith, Joe Orlando, Nanying Bian
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Patent number: 8748582Abstract: The present invention relates generally to affinity ligands and chemical affinity ligand-matrix conjugates for use as chromatographic adsorbents and methods which utilize the adsorbents in the purification of proteins by affinity chromatography. The affinity ligand-matrix conjugates of the present invention comprise ligands of general formula (I): wherein m represents an integer from 0-2, n represents an integer from 0-6, p represents an integer from 0-4, R1 represents H or C1-3 alkyl, R2 is an optional substituent, and X is the position at which the ligand is immobilized, optionally via a linker.Type: GrantFiled: October 15, 2010Date of Patent: June 10, 2014Assignee: Monash UniversityInventors: Milton T W Hearn, Anjali Makarand Bhagwat, William Roy Jackson, Simon John Mountford
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Patent number: 8729244Abstract: The invention relates to a method for purifying factor B, comprising the steps consisting in: (i) obtaining a blood plasma fraction containing factor B; (ii) subjecting the fraction obtained in step (i) to a heparin-like affinity chromatography; (iii) subjecting the factor B-enriched fraction obtained in step (ii) to a cation exchange chromatography; (iv) subjecting the factor B-enriched fraction obtained in step (iii) to an anion exchange chromatography, (v) eluting the factor B.Type: GrantFiled: November 15, 2010Date of Patent: May 20, 2014Assignee: Laboratoire Francais du Fractionnement et des BiotechnologiesInventor: Michel Poulle
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Patent number: 8697844Abstract: The invention relates to a method of purifying PEGylated proteins by removing impurities from samples containing PEGylated proteins, in particular, but not exclusively vitamin K-dependent blood coagulation factors such as Factor IX (FIX), to proteins purified by said method and to the use of said purified proteins in therapy, in particular but not exclusively, for the treatment of diseases alleviated by blood coagulation factors such as the prophylactic treatment of hemophilia.Type: GrantFiled: November 24, 2010Date of Patent: April 15, 2014Assignee: Novo Nordisk A/SInventors: Matthias Karl Dietrich Wiendahl, Lars Sejersgaard, Are Bogsnes
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Patent number: 8685248Abstract: The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.Type: GrantFiled: February 22, 2010Date of Patent: April 1, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman
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Publication number: 20140081008Abstract: The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations.Type: ApplicationFiled: April 2, 2013Publication date: March 20, 2014Applicant: Cytos Biotechnology AGInventor: Cytos Biotechnology AG
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Publication number: 20140081001Abstract: The present invention relates to a process for purification of a target biomolecule, comprising the steps: (a) contacting (i) a target biomolecule, (ii) a dual affinity polypeptide, and (iii) a solid support comprising a catching ligand, wherein the ratio between the equilibrium dissociation constants of the dual affinity polypeptide, [KD,t/KD,s], is at least 100 at standard conditions; and (b) recovering the target biomolecule by elution.Type: ApplicationFiled: November 21, 2013Publication date: March 20, 2014Applicant: NOVOZYMES A/SInventor: Jan Kyhse-Andersen
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Patent number: 8674073Abstract: Provided is affinity chromatography carrier for an immunoglobulin that simultaneously has high immunoglobulin-binding capability and chemical stability and that can be produced at low cost. An immunoglobulin-binding protein in which an amino acid substitution for not only maximizing the number of lysine residues on the protein surface of helix 3 and its periphery but also minimizing the number of lysine residues present on the protein surfaces of helix 1 and helix 2 as immunoglobulin-binding regions and/or an amino acid substitution for eliminating an aspartic acid-proline sequence have (has) been carried out, or a multimer thereof, is used as an affinity ligand for an affinity chromatography carrier.Type: GrantFiled: February 21, 2007Date of Patent: March 18, 2014Assignee: Protenova Co., Ltd.Inventors: Eiji Majima, Atsushi Shima, Yuko Hara
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Patent number: 8669116Abstract: The invention provides a method detecting and quantifying proteins by mass spectrophotometric analysis using peptide internal standards and provides a highly sensitive way of detecting protein modifications. In one aspect, the invention provides a method for determining a site of ubiquitination in a polypeptide and for evaluating ubiquitination targets in a population of polypeptides. In this way, a proteome ubiquitination map can be obtained which comprises information relating to the ubiquitination states of a plurality of cellular polypeptides. Maps can be obtained for a variety of different types of cells and cell states. For example, ubiquitination targets in normal and diseased cells can be evaluated. Preferably, the map is stored as data files in a database. Individual ubiquitinated polypeptides identified can be used to generate molecular probes diagnostic of a cell state and/or can serve as targets for agents that modulate one or more cellular processes.Type: GrantFiled: March 11, 2003Date of Patent: March 11, 2014Assignee: President and Fellows of Harvard CollegeInventors: Steven P. Gygi, Junmin Peng
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Patent number: 8669046Abstract: Antibodies that specifically bind to a peptide having an amino acid sequence as found at the N-terminus of the HA2 fusion peptide of the influenza A virus may be raised by inoculating a mammal with a conjugate of the peptide. In one embodiment, the conjugate comprises the peptide linked to a spacer (e.g. 6-aminocaproic acid) and a carrier protein (e.g. KLH). The antibodies may be used as a universal reagent for detecting HA proteins of influenza viruses. The antibodies are useful as versatile reagents for laboratory research and vaccine potency determination, especially in the event of pandemic influenza outbreaks.Type: GrantFiled: March 10, 2009Date of Patent: March 11, 2014Inventors: Xuguang (Sean) Li, Runtao He, Gary Van Domselaar
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Publication number: 20140065672Abstract: Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification.Type: ApplicationFiled: October 17, 2013Publication date: March 6, 2014Applicant: BOARD TRUSTEES OF THE UNIVERSITY OF ARKANSASInventors: Ellen M. Brune, Robert R. Beitle, JR., Mohammad M. Ataai, Patrick R. Bartlow, Ralph L. Henry
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Patent number: 8663950Abstract: The present invention describes the plant-based production of a therapeutic antibody against West Nile Virus.Type: GrantFiled: January 10, 2011Date of Patent: March 4, 2014Assignee: Arizona Board of RegentsInventors: Qiang Chen, Huafang Lai
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Publication number: 20140058069Abstract: The present invention generally relates to particles, including microgel particles, for purifying proteins and other species. In one aspect, the particles comprise a metal-chelating moiety, which may be distributed substantially evenly throughout the particle in certain embodiments. In some cases, the particles may be porous, and in some embodiments, the particles may be made sufficiently small, for example, in order to form a microgel containing the particles. Such particles may be useful, for example, in binding metal ions (for example, nickel ions) using the metal-chelating moieties. In some embodiments, such particles may also be used to bind certain analytes (for example, proteins) containing tags which attract metal ions, for example, histidine tags. Accordingly, in certain embodiments, the particles may be used for binding or trapping proteins.Type: ApplicationFiled: February 22, 2012Publication date: February 27, 2014Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY, CHILDREN'S MEDICAL CENTER CORPORATIONInventors: Boaz Mizrahi, Daniel S. Kohane
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Patent number: 8658774Abstract: Meditope variants and methods for their use are provided herein. A meditope variant as described herein comprises a peptide having a sequence CQFDLSTRRLKC (SEQ ID NO:1) or CQYNLSSRALKC (SEQ ID NO:2) that has one or more modifications at of least one amino acid residue of the sequence. Multivalent meditope variant tethering entities are also provided. Such entities may include two or more meditopes coupled via a long linker, multivalent scaffold, biotin-streptavidin, or IgG Fc domain. Further, methods of treating, imaging or diagnosing a disease or condition are provided. Such methods may include administering a therapeutically effective amount of a pharmaceutical composition to a subject, the pharmaceutical compound comprising an antibody-meditope complex; a multivalent tethering agent in combination with a monoclonal antibody or functional fragment thereof; or a combination thereof.Type: GrantFiled: October 10, 2011Date of Patent: February 25, 2014Assignee: City of HopeInventors: John C. Williams, David A. Horne, Yuelong Ma, Heng Wei Chang
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Patent number: 8658773Abstract: Disclosed are methods, compositions and uses of high concentration antibody or immunoglobulin formulations for subcutaneous, intramuscular, transdermal or other local (regional) administration, in a volume of than 3, less than 2 or less than 1 ml. Preferably, the formulation contains a high concentration formulation (HCF) buffer comprising phosphate, citrate, polysorbate 80 and mannitol at a pH of about 5.2. The formulation more preferably comprises at least 100, 150, 200, 250 mg/ml or 300 mg/ml of antibody. The methods for preparing the high concentration formulation include ultrafiltration and diafiltration to concentrate the antibody and exchange the medium for HCF buffer. Other embodiments concern use of non-G1m1 (nG1m1) allotype antibodies, such as G1m3 and/or a nG1m1,2 antibodies. The nG1m1 antibodies show decreased immunogenicity compared to G1m1 antibodies.Type: GrantFiled: May 1, 2012Date of Patent: February 25, 2014Assignee: Immunomedics, Inc.Inventors: Li Zeng, Rohini Mitra, Edmund A. Rossi, Hans J. Hansen, David M. Goldenberg
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Patent number: 8652849Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: October 14, 2008Date of Patent: February 18, 2014Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Patent number: 8652330Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: GrantFiled: March 2, 2010Date of Patent: February 18, 2014Assignee: EMD Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin A. Umana, Dennis Aquino, Neil Soice, Andrew Lyddiatt
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Publication number: 20140044796Abstract: The invention concerns the field of detecting and quantifying misfolded proteins/peptides. In particular the detection and quantification of misfolded proteins/peptides in body fluids, on cell surfaces of humans and mammals, the detection of misfolded proteins/peptides in reagents to be tested for scientific research and/or diagnostic use and in pharmaceutical medication or their additives and it concerns as well the removal of misfolded proteins/peptides from reagents to be tested for scientific research and/or for diagnostic purposes and from pharmaceutical medication or their additives.Type: ApplicationFiled: February 10, 2012Publication date: February 13, 2014Applicant: OXPROTECT GMBHInventors: Beate Kehrel, Martin Brodde
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Patent number: 8642745Abstract: The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide.Type: GrantFiled: December 8, 2006Date of Patent: February 4, 2014Assignee: Genentech, Inc.Inventors: W. Robert Arathoon, Paul J. Carter, Anne M. Merchant, Leonard G. Presta
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Publication number: 20140030286Abstract: The present invention relates to a peptide capable of forming a covalent complex consisting either of the Jo peptide, a derivative or a fragment thereof, or of the In peptide, a derivative or a fragment thereof. The present invention relates to the various uses thereof.Type: ApplicationFiled: March 26, 2012Publication date: January 30, 2014Inventors: Thierry Vernet, Anne-Marie Di Guilmi
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Publication number: 20140031532Abstract: Described herein are methods for purifying recombinant, cell culture derived alpha1-protease inhibitor and removing a colored species that co-purifies with the recA1PI protein. Also described are methods for reducing the iron in cell culture derived alpha1-protease inhibitor.Type: ApplicationFiled: March 14, 2013Publication date: January 30, 2014Inventors: DAVID OWNBY, THOMAS P. ZIMMERMAN, JENNIFER A. HUNT, CHARLES MILLER, SENTHIL RANGANATHAN, TONNY DESSOURCES
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Publication number: 20140024052Abstract: An automated protein preparation technology that may use magnetic microparticles to isolate proteins in their native state from specific genomic loci of interest via the chromatin to which they are bound is described. After extraction, the targeted proteins may be purified for down-stream analysis by quantitative mass spectrometry or ELISA. The identification of DNA-bound proteins, histones and their post-translational modification is of high scientific and pharmaceutical importance due to the role of DNA-binding proteins in the causation and development of human disease; in particular, cancer.Type: ApplicationFiled: November 10, 2011Publication date: January 23, 2014Applicants: THE TRUSTEES OF PRINCETON UNIVERSITYInventors: Johannes Dapprich, Benjamin Garcia, Gary Leroy
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Publication number: 20140024813Abstract: A method for separation of an aggregated misfolded protein from an environment including a non-aggregating normal form of the protein includes contacting both the misfolded and normal protein with a conjugated polyelectrolyte (CPE) and separating the CPE/protein complex from the other constituents of the sample.Type: ApplicationFiled: July 17, 2013Publication date: January 23, 2014Applicant: BioChromix ABInventors: Peter ASBERG, Olle INGANAS, Fredrik ANGHUS
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Patent number: 8624004Abstract: The present invention relates to a method for the production of a hepatitis B antigen suitable for use in a vaccine, the method comprising purification of the antigen in the presence of cysteine, to vaccines comprising such antigens.Type: GrantFiled: December 23, 2008Date of Patent: January 7, 2014Assignee: Glaxosmithkline Biologicals, S.A.Inventors: Koen De-Heyder, Peter Schu, Michelle Serantoni, Omer Van-Opstal
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Patent number: 8623594Abstract: The invention includes methods of isolating a multi-subunit protein which binds to an antigen-bearing moiety. The methods comprise generating a phage display library comprising a plurality of virus vectors.Type: GrantFiled: March 26, 2010Date of Patent: January 7, 2014Assignee: The Trustees of the University of PennsylvaniaInventor: Donald L. Siegel
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Publication number: 20140005364Abstract: Sorbent comprising a solid support material, the surface of which comprises a first residue comprising a pyridyl ring, whose hydrogen atoms may be substituted, and a second residue comprising a carboxyl group.Type: ApplicationFiled: December 17, 2010Publication date: January 2, 2014Applicant: INSTRACTION GMBHInventors: Klaus Gottschall, Markus Arendt, Andres Kirschfeld, Christian Meyer, Markus Weis, Martin Welter, Lothar Ziser
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Publication number: 20140004145Abstract: Methods of purifying virus-like particles (VLPs) that are substantially free of process contaminants and infectious agents. The methods incorporate, for example, low-pH treatment during harvest and/or inactivation by a solvent and/or detergent during VLP capture.Type: ApplicationFiled: June 24, 2013Publication date: January 2, 2014Applicant: Takeda Vaccines (Montana), IncInventor: Ross TAYLOR
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Patent number: 8614303Abstract: Methods for establishing a protein expression profile of a biological sample, by contacting a biological sample with a ubiquitin-binding protein such that ubiquitinated and specific non-ubiquitinated protein (the IPS) bind to the ubiquitin-binding protein; isolating the IPS proteins; and analyzing the isolated IPS proteins, wherein an expression profile is generated.Type: GrantFiled: December 11, 2002Date of Patent: December 24, 2013Assignee: University of Medicine and DentistryInventors: Kiran Madura, Li Chen
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Patent number: 8603345Abstract: The invention relates to a device and method for collecting blood whereby certain target components are isolated or removed from the blood sample at the time of collecting the blood, as well as methods for using such devices.Type: GrantFiled: February 13, 2004Date of Patent: December 10, 2013Assignee: Becton, Dickinson and CompanyInventors: Julie Ross, Bruce Haywood
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Publication number: 20130323747Abstract: The present invention relates to a method of detecting the presence, amount or subcellular location of an antigenic structure of interest in a cell.Type: ApplicationFiled: May 20, 2013Publication date: December 5, 2013Applicant: Ludwig-Maximilians-Universitat MunchenInventors: Ulrich Rothbauer, Heinrich Leonhardt