Primers Patents (Class 536/24.33)
  • Patent number: 11053544
    Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
    Type: Grant
    Filed: October 2, 2017
    Date of Patent: July 6, 2021
    Assignees: E. I. DuPont de Nemours and Company, Pioneer Hi-Bred International, Inc., Dow Agrosciences LLC
    Inventors: James Wayne Bing, Robert F. Cressman, Jr., Manju Gupta, Salim M. Hakimi, David Hondred, Todd L. Krone, Mary E. Hartnett Locke, Abigail K. Luckring, Sandra E. Meyer, Daniel Moellenbeck, Kenneth Edwin Narva, Paul D. Olson, Craig D. Sanders, Jimei Wang, Jian Zhang, Gan-Yuan Zhong
  • Patent number: 10323272
    Abstract: The invention provides nucleic acid hybridization probes having improved detectability that include a plurality of first segments consecutively complementary to a target nucleic acid sequence and, between neighboring first segments, a nucleic acid spacer segment which is not complementary to the target nucleic acid sequence and which may include labeled nucleic acid residues. Also provided by the invention are methods for making the probes, methods for using the probes, and compositions of matter that include the probes hybridized to target nucleic acid molecules.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: June 18, 2019
    Assignee: Enzo Biochem, Inc.
    Inventors: Elazar Rabbani, Jack Coleman, Maurizio Mauro
  • Patent number: 9994856
    Abstract: The present invention relates to the field of gene therapy, more specifically to oligonucleotides for making a change in the sequence of a target RNA molecule present in a living cell.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: June 12, 2018
    Assignee: ProQR Therapeutics II B.V.
    Inventors: Daniel Anton De Boer, Tita Ritsema
  • Patent number: 9605255
    Abstract: The present invention relates to the field of gene therapy, more specifically to oligonucleotides for making a change in the sequence of a target RNA molecule present in a living cell.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: March 28, 2017
    Assignee: ProQR Therapeutics II B.V.
    Inventors: Daniel Anton De Boer, Tita Ritsema
  • Patent number: 9410192
    Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: October 14, 2013
    Date of Patent: August 9, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Ma
  • Patent number: 9181582
    Abstract: The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
    Type: Grant
    Filed: June 19, 2013
    Date of Patent: November 10, 2015
    Assignee: NUGEN TECHNOLOGIES, INC.
    Inventor: Nurith Kurn
  • Patent number: 9145557
    Abstract: The present invention relates to oligonucleotides for modulation of target RNA activity. Thus, the invention provides oligonucleotides that bind to microRNA binding sites of target RNA. The oligonucleotides may activate RNase H or RNAi. In a preferred embodiment, the oligonucleotides prevents a micro RNA from binding to its binding site of the target RNA and thereby prevent the microRNA from regulating the target RNA. Such oligonucleotides have uses in research and development of new therapeutics.
    Type: Grant
    Filed: November 23, 2011
    Date of Patent: September 29, 2015
    Assignee: MIRRX THERAPEUTICS A/S
    Inventors: Thorleif Moeller, Christina Udesen
  • Patent number: 9121071
    Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3? terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3? terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.
    Type: Grant
    Filed: March 27, 2014
    Date of Patent: September 1, 2015
    Assignee: Exact Sciences Corporation
    Inventors: Hongzhi Zou, Graham P. Lidgard, Michael J. Domanico, Hatim Allawi
  • Publication number: 20150147757
    Abstract: Methods and compositions for detecting, identifying, and quantifying Brassica A genomic DNA are described. The methods are specific to the Brassica A genome and do not cross-react with other Brassica species, crops or weedy relatives that could contribute to contamination of a canola field.
    Type: Application
    Filed: May 23, 2013
    Publication date: May 28, 2015
    Inventors: Matthew Curtis Harmon, Nancy L. Henderson, Cathy Xiaoyan Zhong
  • Publication number: 20150140564
    Abstract: Disclosed herein is a self-competitive primer for preferentially amplifying a sample nucleic acid based on whether a selected region thereof has a nucleotide variation, in comparison with a selected region of a reference nucleic acid. The self-competitive primer includes a 5?-competing domain and a 3?-elongating domain. Sequences of the 5?-competing domain and the 3?-elongating domain are complementary to a first region and a second region of the reference nucleic acid, respectively. The first region includes the selected region and at least one nucleotide residue immediately downstream of the selected region of the reference nucleic acid. The second region is located downstream of the selected region of the reference nucleic acid and does not comprise the selected region of the reference nucleic acid, and the first region and the second region overlap by at least one nucleotide.
    Type: Application
    Filed: July 3, 2012
    Publication date: May 21, 2015
    Applicant: MACKAY MEMORIAL HOSPITAL
    Inventors: Jr-kai Huang, Chi-Kuan Chen, Tao-Yeuan Wang
  • Publication number: 20150141391
    Abstract: The present disclosure relates to methods of determining a treatment course of action. In particular, the present disclosure relates to mutations in the gene encoding estrogen receptor and their association with responsiveness to estrogen therapies for cancer.
    Type: Application
    Filed: October 14, 2014
    Publication date: May 21, 2015
    Inventors: Arul M. Chinnaiyan, Dan Robinson, Yi-Mi Wu
  • Patent number: 9034837
    Abstract: The present invention provides compositions and methods of treatment of HCV infected subjects that are not sensitive to interferon treatment. Further, compositions and methods are provided for prevention of organ transplant rejection. The compositions of the invention comprise an anti microRNA-122 oligonucleotide, and are made for administration to a primate.
    Type: Grant
    Filed: April 26, 2010
    Date of Patent: May 19, 2015
    Assignee: Roche Innovation Center Copenhagen A/S
    Inventors: Elisabeth S. Hildebrandt-Eriksen, Andreas Petri, Sakari Kauppinen, Niels Abrahamsen, Robert Eldon Lanford
  • Patent number: 9034581
    Abstract: The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: May 19, 2015
    Assignee: ROCHE MOLECULAR SYSTEMS, INC.
    Inventor: Jenny A. Johnson
  • Publication number: 20150132343
    Abstract: A method of treating a patient, comprising administering at least one antibiotic, e.g., doxycycline and ciprofloxacin, sufficient to substantially treat an intracellular bacterial organism present in at least erythrocytes, e.g., over a course of at least two weeks; and subsequently administering at least one immunostimulant, e.g., which directly or indirectly increases levels of immunostimulatory cytokines in the patient, and at least one antioxidant, e.g., glutathione, to effectively treat a coinfection of the patient with a virus. The intracellular bacterial organism may be a rickettsiales-like organism, and the virus may be HIV.
    Type: Application
    Filed: November 10, 2014
    Publication date: May 14, 2015
    Inventor: Luc Montagnier
  • Publication number: 20150133313
    Abstract: The present invention relates to a method for identifying the variety of a hop by using an identification marker comprising at least one single nucleotide polymorphism that differs among varieties, and a method for preparing said identification marker. The present invention also provides a primer or a probe to be used in the method for identifying the variety of a hop, and a nucleic acid of a region including said identification marker. The present invention further provides a method for detecting the intrusion of different varieties in a hop sample.
    Type: Application
    Filed: June 7, 2013
    Publication date: May 14, 2015
    Inventors: Hiromasa Yamauchi, Susumu Furukubo
  • Publication number: 20150133311
    Abstract: The present invention provides a diagnostic agent for endometriosis for measuring the concentration of at least one miRNA selected from the group consisting of hsa-miR-708, hsa-miR-127-3p and hsa-miR-518d-3p in a sample derived from the blood of a subject, the agent containing an amplification primer for the miRNA as a main component. The present invention also provides a method of detecting endometriosis, including a step of measuring the concentration of at least one miRNA selected, from the group consisting of hsa-miR-708, hsa-miR-127-3p and hsa-miR-518d-3p in a sample derived from the blood of the subject. The diagnostic agent for endometriosis of the present invention and the diagnostic method using the agent are simple and low invasive and have high sensitivity and specificity.
    Type: Application
    Filed: May 30, 2013
    Publication date: May 14, 2015
    Inventor: Hidefumi Uchiyama
  • Publication number: 20150132260
    Abstract: The present invention relates to the use of the measure of anelloviral load for the determination of immunosuppression. More precisely, the present invention provides a method for characterizing the immunosuppressed or non-immunosuppressed status of a subject, comprising the steps of determining the anelloviral load from a biological sample of the said subject, and determining from the said comparison the immunosuppressed or non-immunosuppressed status. The determination of the immunosuppressed status of the subject can then be used to design or adapt a therapeutic treatment.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Inventors: Marc Eloit, Justine Cheval, Charles Hebert, Marc Lecuit
  • Publication number: 20150133329
    Abstract: Disclosed herein are methods of detecting influenza virus in a sample from a subject. In some embodiments, the disclosed methods include contacting a sample with at least one primer 10-40 nucleotides in length wherein the at least one primer is capable of hybridizing to an influenza virus polymerase basic protein 1 (PB1) nucleic acid at least 70% identical to the nucleic acid sequence set forth as any one of SEQ ID NOs: 1-3, amplifying the PB1 nucleic acid or a portion thereof to produce an amplified PB1 nucleic acid, and detecting the amplified PB1 nucleic acid, wherein presence of the amplified PB1 nucleic acid indicates presence of influenza virus in the sample from the subject. In some examples, the primers comprise or consist of the nucleic acid sequence set forth as one of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 9, or SEQ ID NO: 10.
    Type: Application
    Filed: March 7, 2013
    Publication date: May 14, 2015
    Inventor: Suxiang Tong
  • Publication number: 20150133317
    Abstract: Disclosed herein are compositions and methods for sequencing, analyzing, and utilizing samples such as single samples. Also disclosed herein are compositions and methods for matching together two or more sequences from a sample. Also disclosed herein are compositions and methods for expressing and screening molecules of interest.
    Type: Application
    Filed: April 27, 2012
    Publication date: May 14, 2015
    Applicants: Department of Veterans Affairs, The Board of Trustees of the Leland Stanford Junior University
    Inventors: William H. Robinson, Yann Chong Tan, Jeremy Sokolove
  • Publication number: 20150133316
    Abstract: The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.
    Type: Application
    Filed: November 25, 2014
    Publication date: May 14, 2015
    Inventors: Matias Kirst, Marcio Fernando Ribeiro De Resende, JR., Leandro Gomide Neves, Christopher Dervinis, Kelly Mayrink Balmant
  • Publication number: 20150132752
    Abstract: The method of the present invention is a method for determining an HLA-A*24 group, which includes determining the HLA-A*24 group on the basis of the results of the typing of (a) a 211st base and (b) a 395th base, in which A (adenine) in the initiation codon for an HLA gene is defined as the 1st base in the genomic DNA of a test subject.
    Type: Application
    Filed: June 26, 2014
    Publication date: May 14, 2015
    Applicant: TOPPAN PRINTING CO., LTD.
    Inventors: Yoichi MAKINO, Akio Yamane, Tomohiko Azuma, Masato Nakayama
  • Patent number: 9029084
    Abstract: A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77.
    Type: Grant
    Filed: April 4, 2006
    Date of Patent: May 12, 2015
    Assignee: Qiagen Manchester Limited
    Inventors: David Mark Whitcombe, Nicola Jo Thelwell, Paul Francis Ravetto
  • Patent number: 9029083
    Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
    Type: Grant
    Filed: October 10, 2005
    Date of Patent: May 12, 2015
    Assignees: Medical Research Council, President and Fellows of Harvard College
    Inventors: Andrew David Griffiths, David Weitz, Darren Link, Keunho Ahn, Jerome Bibette
  • Patent number: 9029103
    Abstract: Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: May 12, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Niall Anthony Gormley, Jonathan Mark Boutell
  • Publication number: 20150125868
    Abstract: The present invention relates to a method for diagnosing a myeloid cancer in a subject, which comprises the step of analyzing a biological sample from said subject by determining the presence or the absence of a mutation in the ASXL1 (additional sex combs like 1) gene coding for the polypeptide having the sequence SEQ ID No 2. A kit for diagnosing myeloid cancer in a subject comprising at least one nucleic acid probe or oligonucleotide or at least one antibody, which can be used in a such a method.
    Type: Application
    Filed: October 10, 2014
    Publication date: May 7, 2015
    Applicants: Qiagen Marseille SA, Institut Paoli-Calmettes, INSERM (Institut National de la Santé et de la Recherche Médicale)
    Inventors: VÉRONIQUE GELSI-BOYER, DANIEL BIRNBAUM
  • Patent number: 9024006
    Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3? terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3? terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: May 5, 2015
    Assignee: Exact Sciences Corporation
    Inventors: Hongzhi Zou, Graham P. Lidgard, Michael J. Domanico, Hatim Allawi
  • Patent number: 9023601
    Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
    Type: Grant
    Filed: September 30, 2011
    Date of Patent: May 5, 2015
    Assignee: Gen9, Inc.
    Inventor: Andrew V. Oleinikov
  • Publication number: 20150119562
    Abstract: The present invention discloses primers for amplifying farnesyl pyrophosphate synthase gene, having sequence selected from the group consisting of Seq Id. nos. 1-3 and 5-7, from mango. Also disclosed herein is a novel nucleotide sequence of sequence ID no. 8 encoding said amplified farnesyl pyrophosphate synthase (FPPS) for enzyme production in an artificial system thus generating the desired flavor in food products.
    Type: Application
    Filed: February 1, 2013
    Publication date: April 30, 2015
    Inventors: Vidya Shrikant Gupta, Ram Shridhar Kulkarni, Sagar Subhash Pandit, Ashok Prabhakar Giri, Keshav H. Pujari
  • Patent number: 9017938
    Abstract: This invention concerns the use of MicroRNA-199b-5p in medical and diagnostic fields. Particularly, this invention concerns the use of the miR199b-5p in the anti-cancer therapy and as a histophatological and metastasis marker.
    Type: Grant
    Filed: March 23, 2010
    Date of Patent: April 28, 2015
    Assignee: Advanced Accelerator Applications
    Inventor: Massimo Zollo
  • Patent number: 9017971
    Abstract: The invention provides improved methods for investigating nucleic acid sequences, wherein at least one additional probe is used which is specific for a (pseudo)gene variant of a target nucleic acid.
    Type: Grant
    Filed: November 5, 2009
    Date of Patent: April 28, 2015
    Assignee: Stichting Sanquin Bloedvoorziening
    Inventors: Taco Willem Kuijpers, Martin de Boer
  • Patent number: 9017947
    Abstract: The present invention provides for soybean plant and seed comprising transformation event MON89788 and DNA molecules unique to these events. The invention also provides methods for detecting the presence of these DNA molecules in a sample.
    Type: Grant
    Filed: August 19, 2011
    Date of Patent: April 28, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Marianne Malven, Jennifer Rinehart, Nancy Taylor, Ellen Dickinson
  • Publication number: 20150111306
    Abstract: The present invention provides a Hemoglobin A1c-specific aptamer and a Hemoglobin-specific aptamer. The aptamers were selected in vitro using SELEX and a microfluidic chip system. The aptamers established low free energy, thus were more stable than conventional antibodies. The high specificity of the aptamers to Hemoglobin A1c or Hemoglobin allows them to be effectively used in diagnosis of diabetes and/or anemia.
    Type: Application
    Filed: March 25, 2014
    Publication date: April 23, 2015
    Applicant: National Tsing Hua University
    Inventors: Gwo-Bin Lee, Shu-Chu Shiesh, Ching-Chu Wu, Hsin-I Lin, Ko-Wei Chang
  • Publication number: 20150111207
    Abstract: Sequences having specificity for Mycoplasma and related Mollicutes genus strains and uses thereof. Methods of use include detection of samples contaminated with Mycoplasma. Kits are provided and comprise one or more oligonucleotides for the detection of Mycoplasma and related Mollicutes genus strains.
    Type: Application
    Filed: May 31, 2013
    Publication date: April 23, 2015
    Inventors: Claudia Zylberberg, Juan Manuel Rodriguez, Andres Clemente Hernando Insua, Ricardo Ariel Lopez
  • Publication number: 20150111789
    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
    Type: Application
    Filed: September 5, 2014
    Publication date: April 23, 2015
    Inventors: Craig Betts, Steve Oh, George Jokhadze
  • Publication number: 20150111206
    Abstract: The invention relates to a method for specifically detecting and optionally for quantifying Mycobacterium avium ssp. paratuberculosis (MAP) in a sample of an individual. According to the method according to the invention, for this purpose the detection of the presence of the IS900 region in a sample is performed by means a nucleic acid amplification and specific oligonucleotides. In a further aspect, the invention relates to a test kit for specifically detecting MAP in a sample by means of amplification methods. Finally, the invention relates to specific oligonucleotides that are suitable for specifically detecting MAP.
    Type: Application
    Filed: April 26, 2013
    Publication date: April 23, 2015
    Inventors: Claus-Peter Czerny, Pia Muenster
  • Publication number: 20150104788
    Abstract: The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.
    Type: Application
    Filed: April 9, 2013
    Publication date: April 16, 2015
    Applicant: ENVIROLOGIX, INC.
    Inventors: Daniel Shaffer, Stephen A. Judice
  • Publication number: 20150105411
    Abstract: Disclosed herein are markers whose mutational status is associated with sensitivity to treatment with NAE inhibitors. Mutational status is determined by measurement of characteristics of markers associated with the marker genes. Compositions and methods are provided to assess markers of marker genes to predict response to NAE inhibition treatment.
    Type: Application
    Filed: October 26, 2012
    Publication date: April 16, 2015
    Applicant: Millennium Pharmaceuticals, Inc.
    Inventors: Stephen J. Blakemore, Bin Li, George J. Mulligan, Matthew C. Schu, Peter G. Smith
  • Patent number: 9000144
    Abstract: In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as “small temporal RNAs” (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs, (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRAN, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
    Type: Grant
    Filed: May 16, 2012
    Date of Patent: April 7, 2015
    Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
    Inventors: Thomas Tuschl, Mariana Lagos-Quintana, Winfried Lendeckel, Jutta Dammann, Reinhard Rauhut
  • Patent number: 8999634
    Abstract: Provided herein are methods and compositions for detection of a nucleic acid target in a sample. The methods and compositions use primer directed amplification in conjunction with nucleic acid fragmentation. The methods have high sensitivity even in the presence of a large amount of non-target nucleic acid. Also provided are oligonucleotides and kits useful in the method. Exemplary nucleic acid targets are those with mutant gene sequence such as mutant sequence of the EGFR, APC, TMPRSS2, ERG and ETV1 genes.
    Type: Grant
    Filed: February 21, 2008
    Date of Patent: April 7, 2015
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Heather R. Sanders, Kevin Z. Qu, Charles M. Strom, Richard A. Bender
  • Patent number: 9000142
    Abstract: There is disclosed a photocleavable sense-antisense nucleobase polymer complex capable of modulating gene expression comprising an unnatural antisense nucleobase polymer that targets an mRNA, and a photocleavable sense nucleobase polymer noncovalently bound to the antisense nucleobase polymer, wherein the photocleavable sense nucleobase polymer comprises a plurality of nucleobase polymers connected by a photocleavable linkage. There is also disclosed a method for controlling the time and spatial position of gene expression comprising selecting a target mRNA, introducing the photocleavable sense-antisense nucleobase polymer complex into a cell, and selectively irradiating the cell with light.
    Type: Grant
    Filed: June 23, 2009
    Date of Patent: April 7, 2015
    Assignee: Syntrix Biosystems, Inc.
    Inventor: John A. Zebala
  • Publication number: 20150093748
    Abstract: The present invention provides a method for detecting the presence of microcystin-producing toxic cyanobacteria in a sample comprising the step of contacting nucleic acid obtained from an environmental sample with primers specifically hybridizing with the nucleic acid sequence of mcy B gene present in Microcystis aeruginosa, Anabaena sp. and Planktothrix agardhii. The present invention further provides primers, primer pairs and probes designed for the method of the invention.
    Type: Application
    Filed: November 9, 2012
    Publication date: April 2, 2015
    Inventors: Henna Savela, Markus Vehniäinen
  • Publication number: 20150096075
    Abstract: The invention overcomes the deficiencies of the prior art by providing methods for marker assisted selection to create plants of a soybean variety that exhibit a mid/low linolenic acid content with a commercially significant yield and an agronomically elite phenotype. The invention also provides derivatives and plant parts of these plants. Further provided by the invention are methods for the use of these plants. The invention is significant in that oil with decreased linolenic acid exhibits numerous beneficial characteristics yet prior art varieties with decreased linolenic acid also exhibited decreased yield and poor agronomic quality.
    Type: Application
    Filed: October 17, 2014
    Publication date: April 2, 2015
    Inventors: Kunsheng Wu, Paul Mclaird, Joseph Byrum, Robert Reiter, Mark Erickson
  • Publication number: 20150093747
    Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
    Type: Application
    Filed: October 15, 2013
    Publication date: April 2, 2015
    Applicants: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI BRED INTERNATIONAL INC
    Inventors: SCOTT DIEHN, ALBERT LU, TIMOTHY NOWATZKI, DOUGLAS NUBEL, M. ALEJANDRA PASCUAL, JAMES C. REGISTER, III, CHRISTOPHER JAY SCELONGE, JOSHUA K. YOUNG, CATHY XIAOYAN ZHONG, ERIN L. CROWGEY
  • Publication number: 20150093787
    Abstract: The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof.
    Type: Application
    Filed: August 5, 2014
    Publication date: April 2, 2015
    Inventors: James HARTLEY, Michael Brasch, Gary Temple, David Cheo
  • Patent number: 8993271
    Abstract: A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provide further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: March 31, 2015
    Assignee: Illumina, Inc.
    Inventors: Eric H. Kawashima, Laurent Farinelli, Pascal Mayer
  • Patent number: 8993238
    Abstract: Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.
    Type: Grant
    Filed: October 7, 2013
    Date of Patent: March 31, 2015
    Assignees: E I du Pont de Nemours and Company, Pioneer Hi Bred International Inc
    Inventors: David George Charne, Wenpin Chen, Chadwick Bruce Koscielny, Zhongsen Li, Jayantilal Patel, Ferdinand G Thoonen, Lomas Tulsieram, Yongping Zhang
  • Publication number: 20150086987
    Abstract: Disclosed herein are methods for detecting presence of one or more of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Toxoplasma gondii, Moraxella catarrhalis, Escherichia coli, Shigella, Staphylococcus aureus, Pneumocystis jirovecii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Ureaplasma spp., Bartonella spp., Streptococcus agalactiae, and Neisseria meningitidis nucleic acids in a sample, such as a biological sample obtained from a subject, or an environmental sample. This disclosure also provides probes, primers, and kits for detecting one or more of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Toxoplasma gondii, Moraxella catarrhalis, Escherichia coli, Shigella, Staphylococcus aureus, Pneumocystis jirovecii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Ureaplasma spp., Bartonella spp., Streptococcus agalactiae, and Neisseria meningitidis in a sample.
    Type: Application
    Filed: February 27, 2013
    Publication date: March 26, 2015
    Inventors: Bernard Wolff, Jonas M. Winchell, Maureen Diaz
  • Patent number: 8986933
    Abstract: A process for detecting human rhinovirus nucleic acid in a biological sample, includes producing an amplification product by amplifying an human bocavirus nucleotide sequence using a forward primer of SEQ ID NO: 1, and a reverse primer of SEQ ID NO: 2, and measuring said amplification product to detect human rhinovirus in said biological sample. Also provided are reagents and methods for detecting and distinguishing human rhinovirus from other viruses. A kit is provided for detecting and quantifying human rhinovirus in a biological sample.
    Type: Grant
    Filed: December 5, 2008
    Date of Patent: March 24, 2015
    Assignee: The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control
    Inventors: Xiaoyan Lu, Dean Erdman
  • Patent number: 8987222
    Abstract: The present invention relates to the discovery of (SNPs) significantly associated with Huntington's disease (HD). The present invention utilizes RNA silencing technology (e.g. RNAi) against such SNPs optimally combined with select additional SNP targeting silencing agents, thereby resulting in an effective treatment of significantly-sized patient populations. Silencing agents having enhanced discriminatory properties are also featured.
    Type: Grant
    Filed: April 8, 2010
    Date of Patent: March 24, 2015
    Assignee: University of Massachusetts
    Inventors: Neil Aronin, Edith Pfister, Phillip D. Zamore
  • Patent number: 8987435
    Abstract: The present invention provides oligomeric compounds and uses thereof. In certain embodiments, such oligomeric compounds are useful as antisense compounds. Certain such antisense compounds are useful as RNase H antisense compounds or as RNAi compounds.
    Type: Grant
    Filed: October 23, 2009
    Date of Patent: March 24, 2015
    Assignee: Isis Pharmaceuticals, Inc.
    Inventors: Eric E. Swayze, Balkrishen Bhat, Walter F. Lima, Thazha P. Prakash, Garth A. Kinberger