Primers Patents (Class 536/24.33)
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Patent number: 8927703Abstract: The present invention provides compositions, methods and kits for the species-specific detection of Pseudomonas aeruginosa.Type: GrantFiled: April 2, 2008Date of Patent: January 6, 2015Assignee: Gen-Probe IncorporatedInventors: Shannon K. Kaplan, Kristin Livezey, Jennifer J. Bungo, James J. Hogan
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Patent number: 8927217Abstract: The present invention relates to methods and kits for the detection of toxic cyanobacteria, in particular of hepatotoxin-producing cyanobacteria.Type: GrantFiled: May 31, 2006Date of Patent: January 6, 2015Assignee: NewSouth Innovations Pty LimitedInventors: Brett A. Neilan, Anne-Dorothee Jungblut
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Patent number: 8921046Abstract: Provided are methods for sequencing a nucleic acid with a sequencing enzyme, e.g., a polymerase or exonuclease. The sequencing enzyme can optionally be exchanged with a second sequencing enzyme, which continues the sequencing of the nucleic acid. In certain embodiments, a template is fixed to a surface through a template localizing moiety. The template localizing moiety can optionally anneal with the nucleic acid and/or associate with the sequencing enzyme. Also provided are compositions comprising a nucleic acid and a first sequencing enzyme, which can sequence the nucleic acid and optionally exchange with a second sequencing enzyme present in the composition. Compositions in which a template localizing moiety is immobilized on a surface are provided. Also provided are methods for using data from analytical reactions wherein two different enzymes are employed, e.g., at a same or different reaction regions.Type: GrantFiled: October 12, 2011Date of Patent: December 30, 2014Assignee: Pacific Biosciences of California, Inc.Inventors: Keith Bjornson, Arek Bibillo, Fred Christians, Kevin Travers, Robin Emig, Stephen Turner
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Patent number: 8921334Abstract: The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Nuclear Respiratory Factor 1 (NRF1), in particular, by targeting natural antisense polynucleotides of Nuclear Respiratory Factor 1 (NRF1). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of NRF1.Type: GrantFiled: December 29, 2010Date of Patent: December 30, 2014Assignee: CuRNA, Inc.Inventors: Joseph Collard, Olga Khorkova Sherman
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Patent number: 8916362Abstract: A polynucleotide device is provided that adds one or more bases to a single stranded polynucleotide. Methods of using the device are provided, comprising contacting a single stranded target molecule with an extension reaction mixture comprising (i) a device or composition of the disclosure, (ii) a polymerase, and (iii) free nucleotides, whereupon an extension reaction product is generated. Kits comprising a device of the disclosure are also provided.Type: GrantFiled: November 23, 2010Date of Patent: December 23, 2014Assignee: Swift Biosciences, Inc.Inventor: Vladimir Makarov
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Publication number: 20140371294Abstract: Isolated histidyl-tRNA synthetase splice variant polynucleotides and polypeptides having non-canonical biological activities are provided, as well as compositions and methods related thereto.Type: ApplicationFiled: April 25, 2014Publication date: December 18, 2014Applicants: aTyr Pharma, Inc., Pangu BioPharma LimitedInventors: Jie Zhou, Ching-Fun Lau, Zhiwen Xu, Wing-Sze Lo, Kristi Helen Piehl, Leslie Ann Greene
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Publication number: 20140370496Abstract: The present invention provides an oligonucleotide for HIV detection including a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6, and an HIV detection kit and an HIV detection method that uses the oligonucleotide for HIV detection.Type: ApplicationFiled: January 24, 2013Publication date: December 18, 2014Applicant: Japan Science and Technology AgencyInventors: Taketoshi Mizutani, Aya Ishizaka
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Publication number: 20140373191Abstract: The invention provides a transgenic Glycine max event MON87751, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event MON87751. The invention also provides polynucleotides specific for event MON87751, plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides for event MON87751. The invention also provides methods related to event MON87751.Type: ApplicationFiled: June 12, 2014Publication date: December 18, 2014Inventors: Kim A. Beazley, Wen C. Burns, Robert H. Cole, II, Ted C. MacRae, John A. Miklos, Lisa G. Ruschke, Kairong Tian, Liping Wei, Kunsheng Wu
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Publication number: 20140369982Abstract: An encapsulation system is provided comprising nitrogen-purge, instant bonding encapsulation method. Specifically, the encapsulation system comprises a composition, a two-piece capsule comprising a capsule cap and a capsule body; a gas to purge oxygen from the composition within the capsule; and a sealing solution to seal the capsule cap to the capsule body. Associated methods for encapsulating compositions using the encapsulation system are also provided.Type: ApplicationFiled: June 19, 2014Publication date: December 18, 2014Inventor: A. Satyanarayan Naidu
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Publication number: 20140370512Abstract: The invention relates to a method for detection of a methicillin resistant coagulase positive Staphylococcus aureus (MRSA) strain by means of a sequence specific amplification reaction.Type: ApplicationFiled: January 23, 2013Publication date: December 18, 2014Inventor: Steffen Mergemeier
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Publication number: 20140371084Abstract: The invention relates to a method for the diagnosis of malignant diseases comprising the steps Provision of a sample obtained from a bodily fluid or bodily excretion as well as Determination of the concentration of snRNA RNU2-1 or their fragments in the sample, as well as a kit for the performance of this diagnosis, and the use of probes specific to RNU2-1 and fragments thereof.Type: ApplicationFiled: July 20, 2012Publication date: December 18, 2014Inventors: Wolff Schmiegel, Alexander Baraniskin, Stephan Hahn
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Patent number: 8911947Abstract: A method is provided which enables quick, convenient, inexpensive, and high sensitivity confirmation of nucleic acid amplification after a nucleic acid amplification reaction. The DNA fragment in accordance with the present invention is a single-stranded DNA fragment containing a hairpin structure which in turn contains a bulge, wherein the DNA fragment is used as being attached to the 5? end of a primer used in nucleic acid amplification. The nucleic acid amplification confirmation method in accordance with the present invention quantifies a hairpin primer containing the DNA fragment at its 5? end by using bulge-binding fluorescent molecules after carrying out PCR or like nucleic acid amplification reaction using the hairpin primer. SNPs are detected quickly and conveniently at low cost and with high sensitivity by applying the nucleic acid amplification confirmation method, for example, to allele specific PCR.Type: GrantFiled: August 28, 2007Date of Patent: December 16, 2014Assignee: Furukawa Electric Advanced Engineering Co., Ltd.Inventors: Kazuhiko Nakatani, Fumie Takei, Masaki Hagihara
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Patent number: 8912154Abstract: The present invention provides pharmaceutical formulations for oral administration of antisense oligonucleotides, such as antisense oligonucleotides against SMAD7. The pharmaceutical formulations can be used to treat Crohn's disease, ulcerative colitis and chronic inflammatory bowel disease.Type: GrantFiled: November 13, 2009Date of Patent: December 16, 2014Assignee: Nogra Pharma LimitedInventors: Sergio Baroni, Salvatore Bellinvia, Francesca Viti
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Publication number: 20140364481Abstract: Provided herein are kits, compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to recurrent RNA fusions as diagnostic markers and clinical targets for leukemia.Type: ApplicationFiled: May 6, 2014Publication date: December 11, 2014Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Kojo Elenitoba-Johnson, Thirunavukkarasu Velusamy, Nallasivam Palanisamy, Anagh Sahasrabuddhe, Megan Lim, Arul Chinnaiyan
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Patent number: 8906614Abstract: Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated.Type: GrantFiled: November 29, 2012Date of Patent: December 9, 2014Assignee: Pacific Biosciences of California, Inc.Inventors: Jeffrey Wegener, Jonas Korlach
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Patent number: 8906876Abstract: The present invention concerns methods for controlling insect infestation via RNAi-mediated gene silencing, whereby the intact insect cell(s) are contacted with a double-stranded RNA from outside the insect cell(s) and whereby the double-stranded RNA is taken up by the intact insect cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from insect pests. Alternatively, the methods are used for treating and/or preventing insect infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable insect target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.Type: GrantFiled: August 23, 2012Date of Patent: December 9, 2014Assignee: Devgen NVInventors: Romaan Raemaekers, Laurent Kubler, Geert Karel Maria Plaetinck, Els Vanbleu, Thierry Andre Olivier Eddy Bogaert
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Publication number: 20140358448Abstract: The present invention is to provide a computer readable medium comprising a computer program for enabling a computer to carry out provision of information on hepatocellular carcinoma in a subject based on an analysis result on methylation status of a novel marker specific for hepatocellular carcinoma. The above object is achieved by obtaining the analysis result on methylation status of a CpG site located in a promoter region of at least one gene selected from CELF6 and RNF135 in a DNA sample derived from the subject and providing information on hepatocellular carcinoma in the subject based on the resulting analysis result.Type: ApplicationFiled: May 28, 2014Publication date: December 4, 2014Applicants: THE UNIVERSITY OF TOKYO, SYSMEX CORPORATIONInventors: Kaya TAI, Genta Nagae, Hiroyuki ABURATANI
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Publication number: 20140357517Abstract: The present disclosure provides a composition for radiation exposure diagnosis including an agent for measuring an expression level of an insulin-like growth factor-binding protein-5 (IGFBP-5) gene at an mRNA or the protein and a kit for radiation exposure diagnosis. Methods of diagnosing radiation exposure as well as methods for screening an agent for enhancing radiation sensitivity or for radiation protection are disclosed. Also provided are compositions for enhancing radiation sensitivity and/or radiation protection.Type: ApplicationFiled: May 9, 2012Publication date: December 4, 2014Applicant: Korea Institute of Radiological & Medical SciencesInventors: Kwang Seok Kim, Sang Woo Bae, Kyu Jin Choi, Eun Sook Kim
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Patent number: 8901095Abstract: The present invention relates to the selective inhibition of protein expression of CAG repeat-related disease proteins such as Huntingtin using nucleic acid analogs. Peptide nucleic acids and locked nucleic acids are particularly useful analogs.Type: GrantFiled: July 28, 2009Date of Patent: December 2, 2014Assignee: The Board of Regents of the University of Texas SystemInventors: David R. Corey, Jiaxin Hu, Masayuki Matsui
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Patent number: 8901287Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods.Type: GrantFiled: October 9, 2009Date of Patent: December 2, 2014Assignee: Qiagen Gaithersburg, Inc.Inventors: Irina Nazarenko, Attila Lorincz, Paul Eder, Brian Lowe, Richard Mallonee, Ha Thai
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Patent number: 8901285Abstract: A polynucleotide comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 3 to 16, 18, 20 to 33, 35 or 37 to 39. A method of detecting the presence or absence of a mutation in the PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, and preferably ARMS primers are combined with Scorpion primers.Type: GrantFiled: September 29, 2008Date of Patent: December 2, 2014Assignee: Qiagen Manchester LimitedInventors: Ruth Board, Jennifer Ferguson, Paul Francis Ravetto, Nicola Jo Thelwell, David Whitcombe
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Publication number: 20140349284Abstract: An object of the present invention is to provide a sample nucleic acid for single nucleotide polymorphism detection which is for use in a simple method for quickly detecting a single nucleotide polymorphism, PCR primers for preparation of a sample for single nucleotide polymorphism detection, and a sample for single nucleotide polymorphism detection which is for use in ion exchange chromatography analysis. The present invention provides a sample nucleic acid for single nucleotide polymorphism detection having the following features: (a) a full chain length of 200 bp or less; (b) a sequence (a tag sequence) incompletely complementary to a template DNA at the 5? or 3? end; and (c) a 10 bp or less difference in chain length from a sample nucleic acid to be compared with to determine the presence of a single nucleotide polymorphism.Type: ApplicationFiled: March 30, 2012Publication date: November 27, 2014Inventors: Takuya Yotani, Eiji Kiyotoh, Koji Ushizawa
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Publication number: 20140349302Abstract: The invention provides a methods and compositions for detecting the presence of viable endophyte in a plant, the method comprising detecting the presence of an endophyte in a young leaf or extract thereof, from the plant, wherein detecting the presence of the endophyte in the young leaf or extract is indicative of the presence of viable endophyte in the plant.Type: ApplicationFiled: September 8, 2012Publication date: November 27, 2014Inventors: Richard David Johnson, Christine Rosalie Voisey
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Patent number: 8895247Abstract: The present invention describes a method for detection of human papillomavirus (HPV) types and a kit for detection of said HPV types.Type: GrantFiled: April 22, 2010Date of Patent: November 25, 2014Assignee: Allum-Jenkins ASInventors: Andrew Jenkins, Anne-Gry Allum, Linda Strand
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Patent number: 8895245Abstract: The invention relates to inhibition of wild-type and certain mutant forms of human histone methyltransferase EZH2, the catalytic subunit of the PRC2 complex which catalyzes the mono- through tri-methylation of lysine 27 on histone H3 (H3-K27). In one embodiment the inhibition is selective for the mutant form of the EZH2, such that trimethylation of H3-K27, which is associated with certain cancers, is inhibited. The methods can be used to treat cancers including follicular lymphoma and diffuse large B-cell lymphoma (DLBCL). Also provided are methods for identifying small molecule selective inhibitors of the mutant forms of EZH2 and also methods for determining responsiveness to an EZH2 inhibitor in a subject.Type: GrantFiled: September 12, 2011Date of Patent: November 25, 2014Assignee: Epizyme, Inc.Inventors: Robert A. Copeland, Victoria M. Richon, Margaret D. Scott, Christopher J. Sneeringer, Kevin W. Kuntz, Sarah K. Knutson, Roy M. Pollock
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Publication number: 20140342355Abstract: The invention relates to a method for the reclassification of a subject to a more appropriate risk assessment to that obtained using the algorithms for such risk estimation such us but not limited to Framingham, Regicor, Score, Procamor Qrisk based on the presence of different polymorphisms. The invention also relates to a method for determining the risk of suffering a cardiovascular disease by combining the absence or presence of one or more polymorphic markers in a sample from the subject with conventional risk factors for CVD as well as computer-implemented means for carrying out said method.Type: ApplicationFiled: August 1, 2012Publication date: November 20, 2014Applicant: Gendiag.exe, S.L.Inventors: Eduardo Salas Pérez-Rasilla, Jaume Marrrugat De La Iglesia, Roberto Elosua Llanos, Sergio Castillo Fernández, Joan Salgado Gómez, José Maria Ordovás Munoz
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Publication number: 20140344997Abstract: Disclosed are polynucleotides encoding polypeptides that comprise the biosynthetic pathway for lignin in the jute plant. The present invention relates generally to the field of plant lignin biosynthesis genes, polypeptides encoded by such genes, and the use of such polynucleotide and polypeptide sequences for controlling plant lignin production. Also disclosed are methods for using the polynucleotides and polypeptides to influence the quality and amount of fiber produced by jute.Type: ApplicationFiled: April 25, 2012Publication date: November 20, 2014Applicant: Bangladesh Jute Research InstituteInventors: Maqsudul Alam, Haseena Khan, Mahboob Zaman, Mohammed K. Uddin, Mohammed S. Haque, Mohammed S. Islam, Muhammed S. Azam
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Patent number: 8889350Abstract: Methods of isolating target double-stranded polynucleotides with internal single-stranded regions are provided. Compositions and kits comprising double-stranded polynucleotides with internal single-stranded regions are also provided.Type: GrantFiled: March 28, 2011Date of Patent: November 18, 2014Assignee: Swift Biosciences, Inc.Inventor: Vladimir Makarov
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Publication number: 20140336241Abstract: Described herein are compositions and methods for prognosis and treatment of prostate cancer patients. Specifically the invention relates to microRNA molecules associated with the prognosis of prostate cancer, as well as various nucleic acid molecules relating thereto or derived therefrom.Type: ApplicationFiled: July 30, 2014Publication date: November 13, 2014Applicant: ROSETTA GENOMICS LTD.Inventors: Ayelet CHAJUT, Shai ROSENWALD
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Publication number: 20140335517Abstract: The present invention provides a method for diagnosing schizophrenia, and a schizophrenia diagnostic reagent or device for use in the method. The present invention further provides a therapeutic or ameliorating agent for schizophrenia, which is effective for the treatment or amelioration of schizophrenia. The therapeutic or ameliorating agent for schizophrenia contains a carbonyl scavenger or a carbonyl-modified protein formation inhibitor as an active ingredient. The method for diagnosing schizophrenia according to the present invention includes measuring at least one parameter in a subject, the parameter being selected from the group consisting of: (1) a genetic abnormality of glyoxalase I gene; (2) the expression level or activity of glyoxalase I in a biological sample; (3) the amount of a carbonyl compound or a carbonyl-modified protein that is a protein modified with the carbonyl compound; and (4) the amount of pyridoxal in a biological sample.Type: ApplicationFiled: July 8, 2014Publication date: November 13, 2014Inventors: Masanari ITOKAWA, Toshio MIYATA, Makoto ARAI
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Patent number: 8883418Abstract: The invention relates to a method for analyzing the diversity of the catalogue of T and/or B lymphocytes in an individual, based on the amplification, from a sample, of genomic DNA fragments by PCR multi-n-plexes, with n?2, carried out with a combination of at least 3 primers defining at least 2 primer couples, each of which includes a primer specifically hybridizing upstream and/or in a given V or D gene and a primer specifically hybridizing downstream and/or in a given J gene, in order to obtain the amplification of at least two fragments characteristic of two distinct V-J or D-J rearrangements from each primer couple. The invention also relates to the applications of this method, in particular in the treatment follow-up or in the diagnosis and/or prognosis of certain diseases.Type: GrantFiled: November 25, 2008Date of Patent: November 11, 2014Assignees: ImmunID, Commissariat a l'Energie Atomique et aux Energies AlternativesInventors: Nicolas Pasqual, Sébastien Weisbuch
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Patent number: 8883994Abstract: A switch mode nucleic acid aptamer probe includes a probe main body, a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main body. The probe main body includes a nucleic acid aptamer fragment with a function of specifically recognizing target tumor cell and a nucleic acid fragment linked to the nucleic acid aptamer fragment by a connection fragment with a length of 7˜15 nm so as to form a hairpin structure. The ability of competitive hybridization of the nucleic acid fragment with the nucleic acid aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specific detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and intravital detection of tumor in living body.Type: GrantFiled: March 22, 2011Date of Patent: November 11, 2014Assignee: Hunan UniversityInventors: Kemin Wang, Hui Shi, Xiaoxiao He, Xiaosheng Ye, Xu Wu, Qiuping Guo, Bing Zhou
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Patent number: 8883423Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.Type: GrantFiled: October 11, 2012Date of Patent: November 11, 2014Assignee: T2 Biosystems, Inc.Inventor: Lori A. Neely
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Patent number: 8883422Abstract: The present invention relates to a new method of establishing the authenticity and origin of dairy products, more specifically to the use of lactic acid bacterial strains having strain-specific insertion sequence elements as tools for marking dairy products (such as cheese) and identification thereof. The invention also extends to new lactic acid bacterial strains, their use in the production of dairy products as well as the dairy products containing these bacterial strains.Type: GrantFiled: October 1, 2010Date of Patent: November 11, 2014Assignee: Agroscope Liebefeld-Posieux ALPInventor: Hélène Berthoud
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Patent number: 8883455Abstract: An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.Type: GrantFiled: September 11, 2013Date of Patent: November 11, 2014Assignee: Gen-Probe IncorporatedInventors: Kelly G. Ammann, Ralph E. Burns, Ernest V. Hansberry, Glenn A. Horner, Cheryl A. Jakub, John E. Kling, Donald J. Nieglos, Robert E. Schneider, Robert J. Smith
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Publication number: 20140330071Abstract: Single nucleotide polymorphic sites of the bovine HSP genes are associated with improved fertilization rate and/or improved embryo survival rate in cattle. Nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods based on these SNPs are disclosed.Type: ApplicationFiled: June 19, 2014Publication date: November 6, 2014Inventor: Hasan Khatib
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Publication number: 20140329245Abstract: Provided herein are methods and compositions for performing PCR with primers with blocked 3?-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations.Type: ApplicationFiled: January 13, 2014Publication date: November 6, 2014Applicant: UniTaq BioInventors: Eugene Spier, Karl Guegler
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Patent number: 8877911Abstract: The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.Type: GrantFiled: June 19, 2012Date of Patent: November 4, 2014Assignee: BASF SEInventors: Chengyan Zhao, Robert Ascenzi, Bijay K. Singh
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Patent number: 8877910Abstract: The present invention relates to probes which detect a polymorphism(s) in exon 12 of the NPM1 gene, a kit therefor, and the method of detecting the polymorphism(s) thereof.Type: GrantFiled: April 27, 2012Date of Patent: November 4, 2014Assignee: Arkray, Inc.Inventor: Toshiya Hosomi
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Patent number: 8877441Abstract: The invention relates to methods and kits for performing in situ hybridization on a biological sample on a solid surface using nucleic acid probes that are embedded in or sorbed to a dry, fibrous matrix.Type: GrantFiled: October 16, 2013Date of Patent: November 4, 2014Assignee: Cellay, Inc.Inventors: Joan Aurich-Costa, Elizabeth Ewen, Michael Gildea
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Publication number: 20140322717Abstract: The present invention provides methods for selectively amplifying a mutant allele of a gene. These methods include (a) contacting a sample containing a mutant and a normal allele of a gene with at least one primer that selectively modifies the normal allele of the gene and causes further amplification of the normal allele to fail or to be substantially reduced; and (b) selectively amplifying the mutant allele of the gene or a portion thereof. Other methods for detecting a mutant allele of a gene in a sample are also provided.Type: ApplicationFiled: November 16, 2012Publication date: October 30, 2014Inventors: Dingbang Xu, Charles Emala
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Publication number: 20140325697Abstract: The present invention relates to the field of plant transformation with genes conferring tolerance to glyphosate. The invention particularly relates to a maize (corn) plant transformed with a gene encoding an EPSPS providing the plant tolerance to an application of glyphosate under conditions where this herbicide is effective in killing weeds. The invention particularly concerns an elite transformation event VCO-Ø1981-5 comprising the gene construct and means, kits and methods for detecting the presence of the said elite event.Type: ApplicationFiled: July 26, 2012Publication date: October 30, 2014Applicant: GenectiveInventors: Lori Artim Artim Mann, Vadim Beilinson, Nadine Carozzi, Rebekah Deter, Brian Vande Berg, Alain Toppan, Laurent Beuf, Georges Freyssinet
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Patent number: 8871444Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalizing genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.Type: GrantFiled: December 4, 2012Date of Patent: October 28, 2014Assignees: Medical Research Council, President and Fellows of Harvard CollegeInventors: Andrew David Griffiths, David A. Weitz, Darren R. Link, Keunho Ahn, Jerome Bibette
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Publication number: 20140315196Abstract: The present invention encompasses methods, assays and kits for the diagnosis, screening and identification of Turner syndrome and other disorders of sexual differentiation in a human using single nucleotide polymorphisms present on the X and Y chromosomes.Type: ApplicationFiled: January 27, 2014Publication date: October 23, 2014Applicant: Yale UniversityInventors: Scott Rivkees, Jeffrey Gruen
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Publication number: 20140317778Abstract: The invention further relates to vectors, host cells, seeds, and plants comprising such a nucleic acid molecule. One aspect of the invention is an isolated antibody or antigen binding fragment thereof that specifically binds to a polypeptide molecule of the present invention. One aspect of the invention is a plane or plant cell transfected by a vector of the present invention. One aspect of the invention relates to isolated nucleic acid molecules and fragments thereof encoding enzymes or proteins involved in disease resistance in jute.Type: ApplicationFiled: June 8, 2012Publication date: October 23, 2014Applicant: BANGLADESH JUTE RESEARCH INSTITUTEInventors: Maqsudul Alam, Haseena Khan, Mahboob Zaman, Mohammed K. Uddin, Mohammed S. Haque, Mohammed S. Islam, Muhammad S. Azam, Niaz Mahmood
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Publication number: 20140315211Abstract: Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression PCR that require, in part, DNA polymerase that lacks 5?-3? exonuclease activity, and a PCR primer, termed a forward selective primer or a nunchaku primer. The methods, assays, and kits provided herein are useful for a wide variety of applications, including cancer screening assays and kits, personalized screening assays, SNP (single nucleotide polymorphism) genotyping and identification, and downstream applications such as next generation high-throughput genomic sequencing and library construction.Type: ApplicationFiled: May 25, 2012Publication date: October 23, 2014Applicant: BRANDEIS UNIVERSITYInventors: Ken Sugino, Serena David, Saori Kato, Sean O'Toole, Sacha Nelson
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Publication number: 20140315750Abstract: A process for detecting norovirus nucleic acid in a sample is provided including producing an amplification product by amplifying a norovirus nucleotide sequence using a forward primer of SEQ ID NO: 1, 4, or 10 and a reverse primer of SEQ ID NO: 2, 5, or 11, and detecting the amplification product to detect norovirus in the sample. Also provided are reagents and methods for detecting and distinguishing GI or Gil norovirus from other infectious agents. A kit is provided for detecting and quantifying norovirus in a sample.Type: ApplicationFiled: November 15, 2012Publication date: October 23, 2014Applicants: and Human ServicesInventors: Jan Vinje, Nicole Gregoricus, Preeti Chhabra, Leslie Barclay
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Publication number: 20140315197Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional “self-digesting” molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5?-5? orientation.Type: ApplicationFiled: February 19, 2014Publication date: October 23, 2014Inventor: Kenneth J. Livak
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Publication number: 20140308674Abstract: The presently disclosed subject matter provides methods of characterizing a melanoma in a subject by measuring amounts of one or more RNAs, miRNAs, and/or polypeptides present in cancer-derived microvesicles isolated from a biological sample from the subject.Type: ApplicationFiled: October 26, 2012Publication date: October 16, 2014Inventors: Hongying Hao, Kelly M. McMasters, Deyi Xiao
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Publication number: 20140308709Abstract: Provided is a polynucleotide including, from the 3? terminus of the polynucleotide to the 5? terminus of the polynucleotide, a first region including a nucleotide sequence complementary to a nucleotide sequence of a portion of a target nucleic acid; a second region including a nucleotide sequence identical to a nucleotide sequence of a portion of the target nucleic acid; and a third region including a nucleotide sequence that self-hybridizes to form a stem-loop structure, and compositions, kits, and methods related thereto.Type: ApplicationFiled: October 10, 2013Publication date: October 16, 2014Inventors: Sea-hee KIM, Joo-won Rhee, Ko-bong Choi