Primers Patents (Class 536/24.33)
  • PROPHYLACTIC OR THERAPEUTIC AGENT FOR ORAL DISEASES
    Publication number: 20140178313
    Abstract: Provided is a novel lactic acid bacterium strain which is capable of suppressing production of volatile sulfur compounds by oral bacteria, has no cariogenicity and no causative role in infective endocarditis, and is safe in an oral cavity, and provided is an agent for preventing, improving and/or treating oral diseases and discomforts by use of the bacterial strain.
    Type: Application
    Filed: August 3, 2012
    Publication date: June 26, 2014
    Applicant: KABUSHIKI KAISHA YAKULT HONSHA
    Inventors: Takekazu Okumura, Tomohiko Terai, Masumi Nakao, Kimiyuki Kaneko, Masahiko Ito, Kouji Miyazaki, Kazuaki Yamaji, Kaoru Tochiya, Nobuhiro Hanada, Susumu Imai, Yoshiaki Nomura, Shunsuke Baba
  • Solid phase sequencing of biopolymers
    Patent number: 8758995
    Abstract: This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Probes may be affixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
    Type: Grant
    Filed: August 6, 2010
    Date of Patent: June 24, 2014
    Assignee: Sequenom, Inc.
    Inventors: Charles R. Cantor, Hubert Koster
  • Methods and compositions for the detection and identification of archaea based on the type II chaperonin (thermosome) gene
    Patent number: 8758999
    Abstract: The present invention relates to primers for the universal amplification and detection of Archaea, which primers are designed based on a multiple sequence alignment of Archaea Type II chaperonin (thermo-some) genes. For detection of Archaea having templates with a GC content of below 60%, primers are designed so that inosine residues are found at degenerate positions. For amplification of higher GC content templates, degenerate positions are replaced with specific nucleotide bases found in the high GC organism. The primers are useful for detecting, identifying and quantifying Archaea in a sample and for determining a phylogenetic relationship of a test Archaea organism.
    Type: Grant
    Filed: March 8, 2011
    Date of Patent: June 24, 2014
    Assignee: University of Saskatchewan
    Inventors: Janet Elizabeth Hill, Bonnie Laura Chaban
  • MUTATIONS IN THE EPIDERMAL GROWTH FACTOR RECEPTOR GENE
    Publication number: 20140170662
    Abstract: The invention relates to a new identified mutation in the epidermal growth factor receptor gene, leading to an amino acidic change which highly correlates with the resistance to a therapy regimen comprising cetuximab and the sensitivity to a therapy regimen comprising panitumumab. The invention includes peptide sequences, primers and probes to detect such a mutation, as well as kits for predicting the response of a subject to a therapy regime comprising cetuximab and/or panitumumab. In particular, the invention is useful in the therapy regimen applicable to metastasic colorectal cancer and to head and neck cancer.
    Type: Application
    Filed: August 2, 2012
    Publication date: June 19, 2014
    Inventors: Clara Montagut Viladot, Joan Albanell Mestres, Ana Rovira Guerin, Beatriz Bellosillo Paricio, Alba Dalmases Massegú
  • METHYLATION SIGNATURE FOR REPLICATIVE SENESCENCE OF CELLS IN CULTURE
    Publication number: 20140170663
    Abstract: A method for determining a replicative senescence status of a cell includes determining a methylation status of at least one CpG-dinucleotide within a region of at least one of about 50,000 bp upstream and downstream of at least one CpG-dinucleotide selected from the group consisting of GRM7-CpG-site #1, CASR-CpG-site #1, PRAMEF2-CpG-site #1, SELP-CpG-site #1, CASP14-CpG-site #1, and KRTAP13-3-CpG-site #1. The methylation status of each of the at least one CpG-dinucleotide determined is compared with a reference methylation status of the respective CpG-dinucleotide.
    Type: Application
    Filed: August 6, 2012
    Publication date: June 19, 2014
    Applicant: RHEINISCH-WESTFAELISCHE TECHNISCHE HOCHSCHULE AACHEN
    Inventors: Wolfgang Wagner, Carmen Koch, Sylvia Joussen, Anne Schellenberg
  • CANCER MARKER, METHOD FOR EVALUATION OF CANCER BY USING THE CANCER MARKER, AND EVALUATION REAGENT
    Publication number: 20140170648
    Abstract: The present invention provides a novel cancer marker for evaluating the onset, the preclinical stage, the clinical stage, or the prognosis of a cancer in a subject, and an evaluation method using the same. A cancer marker containing at least one miRNA selected from hsa-miR-92 and hsa-miR-494 is used as a marker for cancers excluding breast cancer. A method for evaluating the possibility of cancers excluding breast cancer includes the step of detecting the expression level of a cancer marker in a biological sample collected from a subject. In this method, the cancer marker contains at least one miRNA selected from hsa-miR-92 and hsa-miR-494.
    Type: Application
    Filed: September 27, 2013
    Publication date: June 19, 2014
    Applicants: TOKYO MEDICAL UNIVERSITY, NEC CORPORATION
    Inventors: Masahiko KURODA, Masami TANAKA, Kosuke OIKAWA, Takayuki MIZUTANI, Masakatsu TAKANASHI, Seiko IIZUKA, Yoshinori KOSUGI, Kazuma OHYASHIKI, Akihiko TSUCHIDA
  • Primers with Modified Phosphate and Base in Allele-Specific PCR
    Publication number: 20140170650
    Abstract: The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating both a nucleotide with a base covalently modified at the exocyclic amino group and a modified phosphate.
    Type: Application
    Filed: November 27, 2013
    Publication date: June 19, 2014
    Applicant: Roche Molecular Systems, Inc.
    Inventors: Stefanie Froehner, Dieter Heindl, Dirk Kessler, Nancy Schoenbrunner, Alison Tsan
  • Human microRNAs and methods for inhibiting same
    Patent number: 8754203
    Abstract: The invention relates to isolated DNA or RNA molecules comprising at least ten contiguous bases having a sequence in a microRNA shown in SEQ ID NOs: 1-94; 281-374; 467-481; 497-522; or 549, except that up to thirty percent of the bases may be wobble bases, and up to 10% of the contiguous bases may be non-complementary. The invention further relates to modified single stranded microRNA molecules, isolated single stranded anti-microRNA molecules and isolated microRNP molecules. In another embodiment, the invention relates to a method for inhibiting microRNP activity in a cell.
    Type: Grant
    Filed: May 11, 2012
    Date of Patent: June 17, 2014
    Assignee: The Rockefeller University
    Inventors: Thomas Tuschl, Pablo Landgraf
  • CORN EVENT MIR162
    Publication number: 20140162272
    Abstract: A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.
    Type: Application
    Filed: July 11, 2013
    Publication date: June 12, 2014
    Applicant: Syngenta Participations AG
    Inventors: Nykoll Long, Jeffrey Bottoms, Moez Rajabali Meghji, Hope Hart, Qiudeng Que, Derrick Pulliam
  • Transferases and Oxidoreductases, Nucleic Acids Encoding Them and Methods for Making and Using Them.
    Publication number: 20140165221
    Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., d-amino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate<=>pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H2O+acceptor<=>a 2-oxo acid+NH3+reduced acceptor.
    Type: Application
    Filed: February 18, 2014
    Publication date: June 12, 2014
    Applicant: VERENIUM CORPORATION
    Inventors: DAVID P. WEINER, PETER LUGINBUHL, ANALIA BUENO, JOSLIN M. CUENCA, ERIN MARASCO
  • Methods And Compositions For Screening And Treating Developmental Disorders
    Publication number: 20140161721
    Abstract: This document provides methods and materials related to genetic variations of developmental disorders. For example, this document provides methods for using such genetic variations to assess susceptibility of developing Autism Spectrum Disorder.
    Type: Application
    Filed: February 8, 2013
    Publication date: June 12, 2014
    Inventors: Eli Hatchwell, Peggy S. Eis, Stephen Scherer, Aparna Prasad
  • Polynucleotide Primers and Probes
    Publication number: 20140162263
    Abstract: The present invention provides a novel technology that involves improved primer design. These primer pairs have a wide range of applications and provide high sensitivity and specificity.
    Type: Application
    Filed: November 27, 2013
    Publication date: June 12, 2014
    Applicant: SWIFT BIOSCIENCES, INC.
    Inventors: Vladimir Makarov, Sergey V. Chupreta
  • ANNEALING CONTROL PRIMER AND ITS USES
    Publication number: 20140163215
    Abstract: The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3?-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5?-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3?-end portion and said 5?-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.
    Type: Application
    Filed: December 3, 2013
    Publication date: June 12, 2014
    Applicant: SEEGENE, INC.
    Inventor: Jong-Yoon Chun
  • Methods and Compositions for Diagnosing, Prognosing, and Treating Neurological Conditions
    Publication number: 20140162933
    Abstract: This document provides methods and materials related to genetic variations of neurological disorders. For example, this document provides methods for using such genetic variations to assess susceptibility of developing Parkinson's disease.
    Type: Application
    Filed: September 13, 2013
    Publication date: June 12, 2014
    Inventors: Eli Hatchwell, Peggy S. Eis
  • Transcription activator-like effector assembly
    Patent number: 8748134
    Abstract: Described herein are techniques for assembling a polynucleotide encoding a transcription activator-like effector nucleases (TALEN). The techniques ligate and digest necessary modules for a TALEN assembly in one reactor or system. Methods and Kits for generating a TALEN are also described.
    Type: Grant
    Filed: September 26, 2013
    Date of Patent: June 10, 2014
    Assignee: SIDANSAI Biotechnology Co., Ltd
    Inventors: Jinlong Zhao, Zhao Wu
  • Oligonucleotides for detection test of polymorphism of EGFR exon 19 and use therof
    Patent number: 8748590
    Abstract: An oligonucleotide for a detection test of a polymorphism of EGFR exon 19, the oligonucleotide being at least one selected from the group consisting of a P1 oligonucleotide and a P1? oligonucleotide, the P1 oligonucleotide having a 3? end subjected to an extension inhibition treatment, which has an identity of at least 80% with respect to a base sequence including at least the 115th to the 123rd bases of the base sequence indicated in SEQ ID NO: 1 and has a length of from 9 to 80 bases; and the P1? oligonucleotide having a 3? end subjected to an extension inhibition treatment, which hybridizes under stringent conditions with a complementary strand of a base sequence including at least the 115th to the 123rd bases of the base sequence indicated in SEQ ID NO: 1 and having a length of from 9 to 80 bases.
    Type: Grant
    Filed: May 1, 2012
    Date of Patent: June 10, 2014
    Assignee: ARKRAY, Inc.
    Inventor: Aki Iguchi
  • Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences
    Patent number: 8748100
    Abstract: Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.
    Type: Grant
    Filed: August 30, 2007
    Date of Patent: June 10, 2014
    Assignee: The Chinese University of Hong Kong
    Inventors: Yuk Ming Dennis Lo, Yu Kwan Tong, Wai Kwun Rossa Chiu, Chunming Ding
  • DIAGNOSTIC METHODS FOR DETECTING CLOSTRIDIUM DIFFICILE
    Publication number: 20140154692
    Abstract: The invention provides a method of detecting Clostridium difficile in a sample, comprising detecting the presence in said sample of one or more genes that have been identified as being specific to Clostridium difficile. Also provided is a method of diagnosing a Clostridium difficile infection in a subject, a method of determining the efficacy of a therapeutic regime being used to treat a Clostridium difficile infection and a method of testing for the presence of Clostridium difficile in a sample. Further provided are primer pairs and a kit suitable for use in such methods.
    Type: Application
    Filed: June 25, 2012
    Publication date: June 5, 2014
    Applicant: UNIVERSITY OF ULSTER
    Inventors: Nigel G. Ternan, Geoffrey Mcmullan, Christopher I. Gill, Shailesh Jain
  • METHODS AND COMPOSITIONS FOR DIAGNOSING, PROGNOSING, AND TREATING NEUROLOGICAL CONDITIONS
    Publication number: 20140155271
    Abstract: This document provides methods and materials related to genetic variations of neurological disorders. For example, this document provides methods for using such genetic variations to assess susceptibility of developing Parkinson's disease.
    Type: Application
    Filed: November 2, 2012
    Publication date: June 5, 2014
    Applicants: Population Diagnostics, Inc., The Research Foundation of State of University of New York
    Inventors: ELI HATCHWELL, PEGGY S. EIS
  • Panel of micrornas that silence the MCL-1 gene and sensitize cancer cells to ABT-263
    Patent number: 8742083
    Abstract: MicroRNAs (miRNAs) that sensitize cancer cells to Bcl-2 family protein inhibitors are identified and described. Oligonucleotide panels, arrays and methods using the sensitizing miRNAs are also disclosed.
    Type: Grant
    Filed: December 23, 2010
    Date of Patent: June 3, 2014
    Assignee: AbbVie Inc.
    Inventors: Dimitri Semizarov, Xin Lu, Lloyd T. Lam
  • Detection of human papillomavirus
    Patent number: 8741568
    Abstract: There is provided an in vitro method of detecting human papillomavirus nucleic acid in a sample, comprising: (a) contacting said sample with forward and reverse oligonucleotide primers, wherein said primers bind to target sites in the human papillomavirus L1 gene, or the complement thereof, under conditions suitable to promote amplification of a portion of said human papillomavirus L1 gene or complement, thereby generating an amplicon; (b) contacting said amplicon with a probe, wherein the probe binds to a target site within said amplicon; and (c) detecting binding of said probe to said amplicon; wherein said forward primer binds to a target site having the sequence SEQ ID NO: 1; and wherein said reverse primer binds to a target site having the sequence SEQ ID NO: 2.
    Type: Grant
    Filed: February 8, 2008
    Date of Patent: June 3, 2014
    Assignee: Health Protection Agency
    Inventors: Caroline Corless, Malcolm Guiver
  • Gene expression markers for breast cancer prognosis
    Patent number: 8741605
    Abstract: The present invention provides gene sets the expression of which is important in the diagnosis and/or prognosis of breast cancer.
    Type: Grant
    Filed: May 16, 2012
    Date of Patent: June 3, 2014
    Assignee: Genomic Health, Inc.
    Inventors: Melody A. Cobleigh, Steve Shak, Joffre B. Baker, Maureen T. Cronin
  • Method of tagging using a split DBR
    Patent number: 8741606
    Abstract: Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.
    Type: Grant
    Filed: March 29, 2013
    Date of Patent: June 3, 2014
    Assignee: Population Genetics Technologies Ltd.
    Inventors: James Casbon, Sydney Brenner, Robert Osborne, Conrad Lichtenstein, Andreas Claas
  • Oligonucleotide microarray for identification of pathogens
    Patent number: 8741565
    Abstract: A method for detecting a target nucleic acid of a pathogen in a test sample, the method comprising preparing a target nucleic acid detecting reagent and contacting the target nucleic acid detecting reagent with an oligonucleotide microarray. A kit for detecting a target nucleic acid of a pathogen in a test sample is also described. The kit comprises at least one primer pair and an oligonucleotide microarray comprising at least one probe.
    Type: Grant
    Filed: December 28, 2006
    Date of Patent: June 3, 2014
    Assignee: Honeywell International Inc.
    Inventors: Yuandong Gu, Leon Xu
  • METHOD FOR QUANTIFYING HUMAN DNA USING AN INTERNAL CONTROL
    Publication number: 20140147843
    Abstract: The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.
    Type: Application
    Filed: February 20, 2012
    Publication date: May 29, 2014
    Applicants: QIAGEN MANCHESTER LIMITED, QIAGEN GMBH
    Inventors: Francesca Di Pasquale, Holger Engel, Sascha Strauss, Nicola Jo Thel Well
  • Method for isolating high-purity RNA by means of paramagnetic microparticles and nanoparticles
    Patent number: 8735060
    Abstract: The invention relates to a purification method for high-purity, DNA-free RNA using a mixture of nanocarrier beads and paramagnetic beads.
    Type: Grant
    Filed: July 29, 2010
    Date of Patent: May 27, 2014
    Assignee: Stratec Molecular AG
    Inventor: Hans Joos
  • METHODS OF IDENTIFYING INDIVIDUALS AT RISK OF PERIOPERATIVE BLEEDING, RENAL DYSFUNCTION OR STROKE
    Publication number: 20140141429
    Abstract: The present invention relates, in general, to perioperative bleeding and, in particular, to methods of identifying individuals at risk of perioperative bleeding. The present invention relates, in general, to perioperative renal dysfunction and, in particular, to methods of identifying individuals at risk of perioperative renal dysfunction. The present invention relates, in general, to perioperative stroke and, in particular, to methods of identifying individuals at risk of perioperative stroke.
    Type: Application
    Filed: December 6, 2013
    Publication date: May 22, 2014
    Applicant: DUKE UNIVERSITY
    Inventors: Debra A. SCHWINN, Ian Welsby, Mihai V. Podgoreanu, Mark Stafford-Smith, Mark F. Newman, Hilary P. Grocott, William D. White, Richard W. Morris, Joseph P. Mathew
  • Isolated Mammalian Monocyte Cell Genes; Related Reagents
    Publication number: 20140141445
    Abstract: DNA clones encoding a receptor in the Ig superfamily and a related soluble variant have been isolated from a human monocyte library. The invention provides receptor polypeptides, nucleic acids encoding them, expression vectors, and transformed cells for recombinant production of the polypeptides.
    Type: Application
    Filed: January 28, 2014
    Publication date: May 22, 2014
    Applicant: Merck Sharp & Dohme Corp.
    Inventors: Gosse Jan Adema, Linde Meyaard, Daniel M. Gorman, Terrill K. McClanahan, Sandra M. Zurawski, Gerard Zurawski, Lewis L. Lanier, Joseph H. Phillips
  • BCR-ABL1 SPLICE VARIANTS AND USES THEREOF
    Publication number: 20140141427
    Abstract: The present invention is based on BCR-ABL1 splice variants which result from insertion and/or truncation of the bcr-abl1 transcript and the finding that these variants provide resistance to kinase domain inhibitors such as imatinib, nilotinib and dasatinib.
    Type: Application
    Filed: November 11, 2013
    Publication date: May 22, 2014
    Applicant: Quest Diagnostics Investments Incorporated
    Inventor: Wanlong Ma
  • Methods and Compositions for Very High Resolution Genotyping of HLA
    Publication number: 20140141436
    Abstract: The invention is a method of determining HLA genotype for HLA-A, HLA-B, HLA-C, DQB1, DRB1, DRB3, DRB4, DRB5, DPA1 and DPB1. Reagents and kits are also disclosed.
    Type: Application
    Filed: August 21, 2013
    Publication date: May 22, 2014
    Applicant: Roche Molecular Systems, Inc.
    Inventors: Henry Erlich, Bryan Hoglund, Cherie Holcomb, Priscilla Moonsamy
  • METHOD FOR DIAGNOSING ARRHYTHMIA BASED ON SINGLE NUCLEOTIDE POLYMORPHISM IN CHROMOSOME 1Q24, NEURL GENE, OR CUX2 GENE
    Publication number: 20140141987
    Abstract: A method for diagnosing arrhythmia such as atrial fibrillation is provided. A single nucleotide polymorphism present in the region 24 of the long arm of the chromosome 1, NEURL gene, or CUX2 gene is analyzed, and the risk of developing arrhythmia and/or the presence or absence of the onset of arrhythmia is diagnosed on the basis of the analysis result.
    Type: Application
    Filed: March 14, 2012
    Publication date: May 22, 2014
    Applicant: RIKEN
    Inventors: Kouichi Ozaki, Toshihiro Tanaka
  • Isolated Mammalian Monocyte Cell Genes; Related Reagents
    Publication number: 20140141444
    Abstract: DNA clones encoding a receptor in the Ig superfamily and a related soluble variant have been isolated from a human monocyte library. The invention provides receptor polypeptides, nucleic acids encoding them, expression vectors, and transformed cells for recombinant production of the polypeptides.
    Type: Application
    Filed: January 28, 2014
    Publication date: May 22, 2014
    Applicant: Merck Sharp & Dohme Corp.
    Inventors: Gosse Jan Adema, Linde Meyaard, Daniel M. Gorman, Terrill K. McClanahan, Sandra M. Zurawski, Gerard Zurawski, Lewis L. Lanier, Joseph H. Phillips
  • Method of adding a DBR by primer extension
    Patent number: 8728766
    Abstract: Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.
    Type: Grant
    Filed: March 29, 2013
    Date of Patent: May 20, 2014
    Assignee: Population Genetics Technologies Ltd.
    Inventors: James Casbon, Sydney Brenner, Robert Osborne, Conrad Lichtenstein, Andreas Claas
  • Method for the simultaneous determination of blood group and platelet antigen genotypes
    Patent number: 8728735
    Abstract: RBC and platelet (Plt) alloimmunization requires antigen-matched blood to avoid adverse transfusion reactions. Some blood collection facilities use unregulated Abs to reduce the cost of mass screening, and later confirm the phenotype with government approved reagents. Alternatively, RBC and Plt antigens can be screened by virtue of their associated single nucleotide polymorphisms (SNPs). We developed a multiplex PCR-oligonucleotide extension assay using the GenomeLab SNPStream platform to genotype blood for a plurality of blood group antigen-associated SNPs, including but not limited to: RhD (2), RhC/c, RhE/e, S/s, K/k, Kpa/b, Fya/b, FY0, Jka/b, Dia/b, and HPA-1a/b.
    Type: Grant
    Filed: September 1, 2009
    Date of Patent: May 20, 2014
    Assignee: Canadian Blood Services
    Inventor: Gregory A Denomme
  • MIR27B is a novel target for treatment of liver fibrosis
    Patent number: 8729046
    Abstract: Methods are provided for treating fibrosis of a tissue, including fibrosis of the liver, using combinations of antagomirs and/or locked nucleic acids. Compositions therefor are also provided.
    Type: Grant
    Filed: December 6, 2012
    Date of Patent: May 20, 2014
    Assignee: Albert Einstein College of Medicine of Yeshiva University
    Inventors: Leslie E. Rogler, Charles E. Rogler
  • Vaccine for runting-stunting syndrome
    Patent number: 8728484
    Abstract: The present invention includes polypeptides, polynucleotides, antibodies, and vaccines associated with Runting Stunting Syndrome (RSS) in poultry. The present invention also includes diagnostic methods based on such polypeptides, polynucleotides, and antibodies and methods of protecting poultry, including chickens, against RSS by the administration of such polypeptides, polynucleotides, antibodies, and vaccines.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: May 20, 2014
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Egbert Mundt, Holly S. Sellers, Guillermo Zavala
  • HCV GENOTYPE 6 REPLICONS
    Publication number: 20140134604
    Abstract: Replicons of genotype 6 hepatitis C virus (HCV) are provided. These replicons contain adaptive mutations giving rise to the HCV's capability to replicate in vitro. Methods of preparing genotype 6 replicons and methods of using these replicons to screen antiviral agents are also provided.
    Type: Application
    Filed: November 6, 2013
    Publication date: May 15, 2014
    Inventors: Guofeng Cheng, William E. Delaney, IV, Betty Peng, Mei Yu
  • Reagents and methods for detecting HCV
    Publication number: 20140134605
    Abstract: The present disclosure relates to oligonucleotide sequences for amplification primers and their use in performing nucleic acid amplifications of HCV, in particular regions that encode the NS3 polypeptide. In some embodiments the primers are used in nested PCR methods for the detection or sequencing of HCV NS3. The oligonucleotide sequences are also provided assembled as kits that can be used to amplify and detect or sequence HCV NS3.
    Type: Application
    Filed: June 20, 2012
    Publication date: May 15, 2014
    Applicant: SIEMENS HEALTHCARE DIAGNOSTICS INC.
    Inventors: Arejas Uzgiris, Sunil Pandit, Lance Palmer
  • COMPOSITIONS, METHODS, AND SYSTEMS FOR INFERRING BOVINE BREED
    Publication number: 20140134621
    Abstract: Provided herein are methods to discover and use single nucleotide polymorphisms (SNP) for identifying breed, or line and breed, or line composition of a bovine subject. The present invention further provides specific nucleic acid sequences, SNPs, and SNP patterns that can be used for identifying breed or breed combinations for Angus, Holstein, Limousin, Brahman, Hereford, Simmental, Gelbvieh, Charolais and Beefmaster breeds. These patterns can be utilized to manage animals in a feedlot to obtain optimum performance based on known characteristics of specific breeds and identify animals for breeding in selection programs. In another aspect, these patterns can be used to ensure labeling on breed specific branded products.
    Type: Application
    Filed: January 17, 2014
    Publication date: May 15, 2014
    Applicants: Branhaven LLC, Cargill, Incorporated
    Inventors: Sue K. DeNise, Paul Charteris, David Rosenfeld, Tom Holm, Stephen Bates
  • Novel Compositions, Methods and Kits for Enhancing PCR Specificity
    Publication number: 20140134614
    Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.
    Type: Application
    Filed: November 4, 2013
    Publication date: May 15, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Shoulian DONG, Chunmei Liu
  • Direct Molecular Diagnosis of Fetal Aneuploidy
    Publication number: 20140134613
    Abstract: Methods and materials for detection of aneuploidy and other chromosomal abnormalities using fetal tissue are disclosed. Results can be obtained rapidly, without cell culture. The method uses digital PCR for amplification and detection of single target sequences, allowing an accurate count of a specific chromosome or chromosomal region. Specific polynucleic acid primers and probes are disclosed for chromosomes 1, 13, 18, 21, X and Y. These polynucleic acid sequences are chosen to be essentially invariant between individuals, so the test is not dependent on sequence differences between fetus and mother.
    Type: Application
    Filed: November 4, 2013
    Publication date: May 15, 2014
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Hei-Mun Christina Fan, Stephen R. Quake
  • Method for preparing a counter-tagged population of nucleic acid molecules
    Patent number: 8722368
    Abstract: Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.
    Type: Grant
    Filed: March 29, 2013
    Date of Patent: May 13, 2014
    Assignee: Population Genetics Technologies Ltd.
    Inventors: James Casbon, Sydney Brenner, Robert Osborne, Conrad Lichtenstein, Andreas Claas
  • Isolated genomic polynucleotide fragments from the p15 region of isolated genomic polynucleotide fragments from the p15 region of chromosome 11 encoding human tumor suppressing subtransferable candidate 4 (TSSC4)
    Patent number: 8722865
    Abstract: Provided herein are isolated genomic polynucleotide fragments from the from the p15 region of chromosome 11 encoding human and tumor suppressing subtransferable candidate 4 (TSSC4) and methods of use.
    Type: Grant
    Filed: September 24, 2011
    Date of Patent: May 13, 2014
    Inventor: James Ryan
  • MUTATIONAL PROFILE IN HIV-1 GAG CLEAVAGE SITE CORRELATED WITH PHENOTYPIC DRUG RESISTANCE
    Publication number: 20140127797
    Abstract: The invention concerns novel mutations or mutational profiles of HIV-1 protease cleavage sites (CS) in the Gag region correlated with a phenotype causing alterations in sensitivity to anti-HIV drugs. The present invention also relates to the use of genotypic characterization of a target population of HIV and the subsequent association, i.e., correlation, of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention further relates to methods of utilizing the mutational profiles of the invention in databases, drug development, i.e., drug design, and drug modification, therapy and treatment design and clinical management.
    Type: Application
    Filed: August 21, 2013
    Publication date: May 8, 2014
    Applicant: JANSSEN R&D IRELAND
    Inventors: INGE DIERYNCK, SANDRA DE MEYER
  • PRIMERS AND PROBES FOR DETECTION AND DISCRIMINATION OF TYPES AND SUBTYPES OF INFLUENZA VIRUSES
    Publication number: 20140128279
    Abstract: Methods of detecting influenza, including differentiating between type and subtype are disclosed, for example to detect, type, and/or subtype an influenza infection. A sample suspected of containing a nucleic acid of an influenza virus, is screened for the presence or absence of that nucleic acid. The presence of the influenza virus nucleic acid indicates the presence of influenza virus. Determining whether the influenza virus nucleic acid is present in the sample can be accomplished by detecting hybridization between an influenza specific probe, influenza type specific probe, and/or subtype specific probe and an influenza nucleic acid. Probes and primers for the detection, typing and/or subtyping of influenza virus are also disclosed. Kits and arrays that contain the disclosed probes and/or primers also are disclosed.
    Type: Application
    Filed: October 17, 2013
    Publication date: May 8, 2014
    Applicant: The Government of the United States of America as represented by the Secretary of the Department of
    Inventors: Stephen Lindstrom, Alexander Klimov, Nancy Cox, Lamorris Loftin
  • GENETIC POLYMORPHISMS ASSOCIATED WITH VENOUS THROMBOSIS AND STATIN RESPONSE, METHODS OF DETECTION AND USES THEREOF
    Publication number: 20140128362
    Abstract: The present invention provides compositions and methods based on genetic polymorphisms that are associated with response to statin treatment (particularly for reducing the risk of venous thrombosis). For example, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by these nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and variant proteins, and methods of using the nucleic acid molecules and proteins as well as methods of using reagents for their detection.
    Type: Application
    Filed: March 20, 2013
    Publication date: May 8, 2014
    Applicant: CELERA CORPORATION
    Inventors: Lance BARE, James J. DEVLIN, Frits R. ROSENDAAL, Pieter H. REITSMA, Irene D. BEZEMER
  • Standardized method and kit for the quantification of hepatitis A virus
    Patent number: 8715935
    Abstract: The present invention provides a standardized method and a kit for an accurate quantification of HAV in clinical and food samples. The general approach is based on the use of several controls to measure the efficiency of those critical steps of the quantification: the nucleic acids extraction and the RT-PCR reactions. The kit comprises: a Mengo virus mutant strain with the same growth properties than those of the wild-type Mengo virus and with no pathogenic capacity; a single stranded RNA molecule corresponding to a fragment of the HAV genome; primers that specifically bind to regions of the 5? non coding region of the HAV genome; a detectable labeled probe that specifically binds to the amplimer resulting from the RT-PCR; and an appropriate molecule to generate an standard curve for the quantification of HAV.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: May 6, 2014
    Assignee: Universidad de Barcelona
    Inventors: Albert Bosch Navarro, Rosa María Pintó Solé
  • Method of determining types I, II, III, IV or V or methicillin-resistant (MRSA) in a biological sample
    Patent number: 8715936
    Abstract: Disclosed are diagnostic methods for determining a subtype of methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample of a mammal. Methods include providing a biological sample of the mammal, performing a PCR analysis of the biological sample, and analyzing the PCR amplicons with respect to their sizes so as to determine for type I, type II, type III, type IV or type V MRSA that may be present in the biological sample. Further example embodiments include using at least one mecA primer pair and/or using at least one Staphylococcus aureus nuc primer pair in the PCR analysis. Further disclosed are methods for screening populations for MRSA, and methods of treating a mammal testing positive for Type IV MRSA. Also disclosed are kits for determining a MRSA subtype in a mammal and isolated primers that may be used in the present methods and kits.
    Type: Grant
    Filed: January 13, 2011
    Date of Patent: May 6, 2014
    Assignee: Medical Diagnostic Laboratories, LLC
    Inventors: Scott E. Gygax, Christina Lim Overmyer, Lisa A. DeSalvia, Martin E. Adelson, Eli Mordechai
  • Method for accurately counting starting molecules
    Patent number: 8715967
    Abstract: Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.
    Type: Grant
    Filed: March 29, 2013
    Date of Patent: May 6, 2014
    Assignee: Population Genetics Technologies Ltd.
    Inventors: James Casbon, Sydney Brenner, Robert Osborne, Conrad Lichtenstein, Andreas Claas
  • Inhibition of DNA polymerases to augment chemotherapeutic and antimicrobial agents
    Patent number: 8715927
    Abstract: Disclosed herein is the identification of human DNA polymerase ? (pol ?) as the polymerase that mediates repair of DNA containing interstrand cros slinks (ICLs). The mechanism of action of a number of chemotherapeutic and antimicrobial agents is the induction of ICLs. Thus, provided herein is a method of enhancing the efficacy of a chemotherapeutic or antimicrobial agent in a subject, including selecting a subject in need of treatment with an ICL -inducing agent and administering to the subject an ICL-inducing agent and a therapeutically effective amount of an inhibitor of pol ?. Also provided is a composition for treating a hyperproliferative disease, an autoimmune disease or an infectious disease, comprising an ICL-inducing agent and an amount of an inhibitor of pol ? sufficient to enhance the efficacy of the ICL-inducing agent. Further provided is a method of identifying a DNA polymerase inhibitor.
    Type: Grant
    Filed: April 22, 2009
    Date of Patent: May 6, 2014
    Assignee: Oregon Health & Science University
    Inventors: R. Stephen Lloyd, Irina G. Minko, Amanda K. McCullough