Synthesis Of Polynucleotides Or Oligonucleotides Patents (Class 536/25.3)
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Publication number: 20110178285Abstract: Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.Type: ApplicationFiled: January 18, 2011Publication date: July 21, 2011Inventors: Michal Lebl, Michel Perbost, Chad F. DeRosier, Mark J. Nibbe, Steve R. Burgett, David L. Heiner
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Patent number: 7982030Abstract: The present invention provides selenium derivatives of nucleosides, nucleoside phosphoramidites, nucleotides, nucleotide triphosphates, oligonucleotides, polynucleotides, and larger nucleic acids and methods for their synthesis. Selenium derivatives of both ribonucleic acids and deoxyribonucleic acids, as well as methods for their synthesis, crystallization and uses in structural determinations, particularly by X-ray crystallographic techniques are disclosed. The selenium derivatives of the present invention are also useful as food supplements.Type: GrantFiled: September 18, 2009Date of Patent: July 19, 2011Assignee: Georgia State University Research Foundation, Inc.Inventor: Zhen Huang
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Patent number: 7981608Abstract: Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.Type: GrantFiled: May 15, 2007Date of Patent: July 19, 2011Assignees: Hitachi Chemical Co., Ltd., Hitachi Chemical Research CenterInventor: Masato Mitsuhashi
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Patent number: 7983849Abstract: The invention relates to methods of determining the mutational load of a gene bank obtained by random mutagenesis of a gene of interest by preparing a chart linking the mutational load of a gene bank obtained by random mutagenesis of a model gene with the fraction of mutated model genes observed in the bank; performing, in parallel, random mutagenesis of the model gene used for preparing the chart and the gene of interest to obtain the corresponding mutated gene banks; determining the mutational load of the gene bank obtained using the model gene on the basis of the chart plotted; and applying a correction factor to the mutational load of the mutated model gene bank to determine the mutational load in the gene bank of the mutated genes of interest.Type: GrantFiled: June 7, 2005Date of Patent: July 19, 2011Assignee: Proteus S.A.Inventors: Matthieu Chodorge, Laurent Fourage, Fabrice Lefevre, Jean-Michel Masson
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Patent number: 7982029Abstract: This invention provides a process for making 3?-O-allyl-dGTP-PC-Biodopy-FL-510, 3?-O-allyl-dATP-PC-ROX, 3?-O-allyl-dCTP-PC-Bodipy-650 and 3?-O-allyl-dUTP-PC-R6G, and related methods.Type: GrantFiled: October 31, 2006Date of Patent: July 19, 2011Assignee: The Trustees of Columbia University in the City of New YorkInventors: Jingyue Ju, Qinglin Meng, Dae H. Kim, Lanrong Bi, Xiaopeng Bai, Nicholas J. Turro
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Publication number: 20110171218Abstract: Provided herein are glycovariant Fc fusion proteins having increased serum half lives. Also provided are methods for increasing the serum half life of an Fc fusion protein by introducing one or more non-endogenous glycosylation sites.Type: ApplicationFiled: December 2, 2010Publication date: July 14, 2011Applicant: Acceleron Pharma Inc.Inventors: Jasbir Seehra, John Knopf, Ravindra Kumar
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Publication number: 20110165226Abstract: The invention comprises a viral cap-leader sequence which comprises at least three bases complementary to the 3?-ultimate residues of a (?)ssRNA virus template sequence. Preferably said consensus sequence is represented by the general formula 7mG(N)6-10-(A/U/G)?A/U?AGC, more preferably 7mG(N)7-8-(A/U/G)?A/U?AGC. Further comprised are modified cap-leader sequences that are able to block elongation by the viral polymerase or cleavage by the viral endonuclase. Such modified cap-leader sequences comprise a 3?-terminal phosphate group, one or more phosphorothioate or phosphorodiamidate bonds or one ore more morpholino rings. Also pharmaceutical products comprising such a modified cap-leader sequence are disclosed. Further, the invention comprises methods for inhibiting virus transcription, especially for influenza virus, using such a modified cap-leader sequence or pharmaceutical product.Type: ApplicationFiled: May 20, 2009Publication date: July 7, 2011Inventor: Richard Jozef Maria Kormelink
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Publication number: 20110165630Abstract: The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, said method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5? ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5? region that is identical to sequence on the target nucleic acid, a 3? region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5? and 3? regions that is not identical to sequence on the target N nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selectType: ApplicationFiled: February 20, 2009Publication date: July 7, 2011Applicant: Gene Bridges GmbHInventors: Marcello Maresca, Axel Steffen Erler, Jun Fu, Philipp Martin Seibert, Adrian Francis Stewart, Youming Ahang
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Patent number: 7972817Abstract: This invention relates to the use of extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood. Specifically, this invention enables the extraction of circulating RNA from plasma or serum and utilizes nucleic acid amplification assays for the identification, detection, inference, monitoring, or evaluation of any such RNA. Further, this invention allows the qualitative or quantitative detection of extracellular RNA circulating in the plasma or serum of humans.Type: GrantFiled: August 31, 2005Date of Patent: July 5, 2011Assignee: OncoMedx, Inc.Inventor: Michael Kopreski
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Publication number: 20110160076Abstract: Disclosed herein are uniquely specific nucleic acid probes and methods for their use and production. The disclosed probes have reduced or eliminated background signal while reducing or eliminating the use of blocking DNA during hybridization. In one example, probes are produced by a method that includes joining at least a first binding region and a second binding region in a pre-determined order and orientation, wherein the first binding region and second binding region are complementary to uniquely specific nucleic acid sequences, wherein the uniquely specific nucleic acid sequences are represented only once in a genome of an organism and wherein the first binding region and the second binding region include about 20% or less of a genomic target nucleic acid molecule. In particular examples, the binding regions (“uniquely specific binding regions”) are complementary to non-contiguous portions of the genomic target nucleic acid.Type: ApplicationFiled: December 30, 2010Publication date: June 30, 2011Inventors: Nelson Alexander, Stacey Stanislaw, James Grille, Mark B. Leick
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Patent number: 7968317Abstract: This invention provides methods for detecting the presence of malignant or premalignant cells, or trophoblastic cells in a human wherein the malignant, premalignant or trophoblastic cells express 5T4. The methods of the invention detect 5T4 RNA in blood, blood plasma, serum, and other bodily fluids. The inventive methods are useful for detection, diagnosis, monitoring, treatment, or evaluation of neoplastic disease, and for the detection and evaluation of placental tissue in pregnant women.Type: GrantFiled: May 2, 2006Date of Patent: June 28, 2011Assignee: OncoMedx, Inc.Inventors: Michael S. Kopreski, Christopher D. Gocke
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Patent number: 7968701Abstract: The present invention provides a polyribonucleoside ladder copolymer molecule of general formula (I)Type: GrantFiled: August 18, 2008Date of Patent: June 28, 2011Assignee: New York UniversityInventors: Yu Liu, Liang Ding, Ruojie Sha, Nadrian Seeman, James Canary
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Patent number: 7964345Abstract: Probes and methods of using the probes to detect chromosomal rearrangements and/or deletions are provided. The methods utilize probes that are free of repeat sequences to provide greater selectivity and sensitivity; methods for producing such probes are also disclosed. The probe sets utilized in the detection methods are designed to hybridize to chromosomes at regions outside known breakpoints, instead of spanning the breakpoint as with conventional FISH methods, and, in some instances, are further designed to bind to regions located outside the genes involved in the rearrangement. Methods utilizing probe sets with two and four colors are also described, as are automated methods for analyzing rearrangements.Type: GrantFiled: April 5, 2005Date of Patent: June 21, 2011Assignee: Cancer Genetics, Inc.Inventors: Nallasivam Palanisamy, Raju S. Chaganti
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Patent number: 7964580Abstract: Disclosed herein are phosphoramidate prodrugs of nucleoside derivatives for the treatment of viral infections in mammals, which is a compound, its stereoisomer, salt (acid or basic addition salt), hydrate, solvate, or crystalline form thereof, represented by the following structure: Also disclosed are methods of treatment, uses, and processes for preparing each of which utilize the compound represented by formula I.Type: GrantFiled: March 21, 2008Date of Patent: June 21, 2011Assignee: Pharmasset, Inc.Inventors: Michael Joseph Sofia, Jinfa Du, Peiyuan Wang
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Publication number: 20110144319Abstract: Disclosed, among other things, are primers containing certain modified nucleobases in the 3? terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.Type: ApplicationFiled: September 23, 2010Publication date: June 16, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Khairuzzaman Bashar Mullah, Zhaochun Ma
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Patent number: 7960526Abstract: The present invention provides for reagents for the introduction of colorimetric-oxycarbonyl protecting groups, compounds bearing colorimetric-oxycarbonyl protecting groups, and the use thereof in solid-supported organic syntheses of oligonucleotides, polypeptides, polysaccharides, and combinatorial libraries.Type: GrantFiled: March 30, 2009Date of Patent: June 14, 2011Assignee: Berry and Associates, Inc.Inventors: John C. Hodges, Yam Foo Poon, William H. Pearson
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Publication number: 20110137022Abstract: A method for the analysis of oligonucleotides is provided. The method comprises: desalting a mixture of oligonucleotides under conditions such that there is substantially no separation of the mixture of oligonucleotides, and/or by on-line desalting, introducing the desalted mixture of oligonucleotides into a mass spectrometer; and quantifying one or more oligonucleotides comprised in the mixture by mass spectrometry.Type: ApplicationFiled: August 7, 2009Publication date: June 9, 2011Inventors: Dennis Paul Michaud, Paul McCormac
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Patent number: 7956011Abstract: Materials and methods for photodirected synthesis of oligonucleotide arrays on a solid substrate by photodirected synthesis are disclosed which employ a film formed from (i) a photoacid generator that on photolysis generates acid that is capable of directly removing the protecting group of the linker molecules or oligonucleotides and (ii) a polymer substantially lacking electronegative heteroatoms that are capable of hydrogen bonding with photogenerated acid. Methods of synthesizing an oligomer arrays are also described that use a film that restricts diffusion of reactants and products on the substrate during synthesis of the array and which includes a precursor of a deprotecting reagent. The method involves removing one or more of the non-required products of the deprotection reaction from the reactive array elements at which they are produced during the reaction, in order to displace the deprotection reaction towards completion.Type: GrantFiled: May 4, 2006Date of Patent: June 7, 2011Assignee: The Institute of Cancer Research: Royal Cancer HospitalInventors: Pawel Jerzy Serafinowski, Peter Bryan Garland
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Patent number: 7951533Abstract: Novel technologies are provided to determine the structure of and produce a functional substance that has a high affinity to a target. Candidates for a substance having an affinity to a target (functional substance) are synthesized; a functional substance that has an affinity to the target is selected from among the functional substance candidates; a specific substituent group is eliminated from the selected functional substance; the functional substance from which the specific substituent group has been eliminated is amplified; the structure of the amplified functional substance is determined; and the functional substance is produced, based on the structure.Type: GrantFiled: October 31, 2005Date of Patent: May 31, 2011Assignee: Fujitsu LimitedInventors: Tsuyoshi Fujihara, Shozo Fujita
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Patent number: 7951926Abstract: Method for the synthesis of nucleotide derivatives wherein molecules of interest are grafted on the oligonucleotide with the help of a “click chemistry” reaction between an azide function on the molecule of interest and an alkyne function on the oligonucleotide, or between an alkyne function on the molecule of interest and an azide function on the oligonucleotide. Intermediate molecules, notably alkyne functionalized oligonucleotides, grafted oligonucleotides, azide functionalized oligonucleotides, oligonucleotide micro arrays containing them and the use of those grafted oligonucleotides for biological investigation and for cell targeting.Type: GrantFiled: April 26, 2007Date of Patent: May 31, 2011Assignee: Centre National de la Recherche Scientifique (CNRS)Inventors: François Morvan, Albert Meyer, Jean-Jacques Vasseur, Sébastien Vidal, Jean-Pierre Cloarec, Yann Chevolot, Eliane Souteyrand
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Patent number: 7951753Abstract: Libraries of nucleic acids encoding chimeric binding polypeptides based on plant scaffold polypeptide sequences. Also described are methods for generating the libraries.Type: GrantFiled: February 13, 2007Date of Patent: May 31, 2011Assignee: Divergence, Inc.Inventor: Jennifer Jones
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Patent number: 7947817Abstract: The invention provides compositions that comprise nucleotides and/or nucleosides having blocking groups at 2?-positions of sugar moieties. Methods of synthesizing these nucleic acids are also provided.Type: GrantFiled: June 28, 2004Date of Patent: May 24, 2011Assignee: Roche Molecular Systems, Inc.Inventors: Veeraiah Bodepudi, Stephen Gordon Will, David Harrow Gelfand
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Publication number: 20110119778Abstract: The invention relates to the storage of information in nucleic acid sequences. The invention also relates to nucleic acid sequences containing desired information and to the design, production or use of sequences of this type.Type: ApplicationFiled: November 28, 2008Publication date: May 19, 2011Inventor: Michael Liss
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Publication number: 20110117544Abstract: There is a method for converting at least a part of a target polynucleotide sequence into a different sequence, comprising the steps of: (i) hybridising two or more first polynucleotides to adjacent positions on the target and linking first polynucleotides together, wherein at least one of the first polynucleotides comprises at least one nucleotide that is not complementary to the target; and (ii) dissociating the hybrid of step (i) and forming a second polynucleotide hybridised to the first polynucleotide, the second polynucleotide comprising at least one portion complementary to a portion on the first polynucleotide and comprising the complement to the at least one nucleotide of step (i).Type: ApplicationFiled: March 1, 2006Publication date: May 19, 2011Applicant: LINGVITAE ASInventor: Preben Lexow
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Publication number: 20110118443Abstract: Methods for designing optimized antibodies, including optimized humanized or human antibodies, to target bioactive lipids are provided. These methods may be performed in silico and may be intended to enhance binding affinity of an antibody to its original target lipid, and/or to alter binding specificity. Antibodies produced by these methods are also provided, as are methods for using them.Type: ApplicationFiled: June 4, 2010Publication date: May 19, 2011Inventors: Roger A. SABBADINI, Tom HUXFORD, Jonathan Michael WOJCIAK
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Publication number: 20110118454Abstract: The invention concerns a method for preparing monomer nucleotides or nucleotide analogues comprising the steps of: (1) coupling a soluble polyethylene glycol support provided with at least one diacid or ether-acid linker and a monomer nucleoside or nucleoside analogue to an amine group or hydroxyl group of the nucleoside with the aid of a coupling agent; (2) at least one step for phosphorylation of said nucleoside or nucleoside analogue coupled to said support with a phosphorylation agent; (3) cleavage of said support and recovery of at least one monomer nucleotide or nucleotide analogue. The nucleotides prepared are biological tools.Type: ApplicationFiled: February 23, 2009Publication date: May 19, 2011Inventors: Suzanne Peyrottes, Christian Perigaud, Céline Crauste
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Patent number: 7943751Abstract: The present invention relates to a copolymer termed a ladder copolymer because it has two backbones that serve as legs/sides of a ladder structure. These two backbones, one of which is a nucleic acid or nucleic acid-like polymer, are linked together as the legs/sides of a ladder are linked together by the rungs.Type: GrantFiled: December 8, 2008Date of Patent: May 17, 2011Assignee: New York UniversityInventors: James Wayne Canary, Nadrian C. Seeman, Lei Zhu, Philip Lukeman
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Publication number: 20110110968Abstract: The invention relates to a humanized nucleic acid construct from Bacillus anthracis protective antigen (PA) gene and method of modifying the gene. The humanized gene, and method of producing it, improves the structural fidelity of expressed protein product, when produced in mammalian host cells, to native, bacterially produced protein. The construct is useful in nucleic acid based vaccine formulations against B. anthracis.Type: ApplicationFiled: November 1, 2010Publication date: May 12, 2011Inventors: Stanley Goldman, Mark Albrecht
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Publication number: 20110112285Abstract: The preparation of oligonucleotides having a 5?-phosphate monoester end.Type: ApplicationFiled: April 15, 2009Publication date: May 12, 2011Applicant: UNIVERSITE JOSEPH FOURIERInventors: Eric Derfrancq, Remi Lartia
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Patent number: 7939300Abstract: Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.Type: GrantFiled: May 15, 2007Date of Patent: May 10, 2011Assignees: Hitachi Chemical Co., Ltd, Hitachi Chemical Research CenterInventor: Masato Mitsuhashi
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Publication number: 20110097707Abstract: One aspect of the present invention relates to an oligonucleotide agent comprising at least one universal nucleobase. In certain embodiments, the universal nucleobase is difluorotolyl, nitroindolyl, nitropyrrolyl, or nitroimidazolyl. In a preferred embodiment, the universal nucleobase is difluorotolyl. In certain embodiments, the oligonucleotide is double-stranded. In certain embodiments, the oligonucleotide is single-stranded. Another aspect of the present invention relates to a method of altering the expression level of a target in the presence of target sequence polymorphism. In a preferred embodiment, the oligonucleotide agent alters the expression of different alleles of a gene. In another preferred embodiment, the oligonucleotide agent alters the expression level of two or more genes. In another embodiment, the oligonucleotide agent alters the expression level of a viral gene from different strains of the virus.Type: ApplicationFiled: October 29, 2010Publication date: April 28, 2011Applicant: ALNYLAM PHARMACEUTICALS, INC.Inventors: Muthiah MANOHARAN, Kallanthottathil G. RAJEEV
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Patent number: 7932025Abstract: This invention generally relates to nucleic acid synthesis, in particular DNA synthesis. More particularly, the invention relates to the production of long nucleic acid molecules with precise user control over sequence content. This invention also relates to the prevention and/or removal of errors within nucleic acid molecules.Type: GrantFiled: December 10, 2003Date of Patent: April 26, 2011Assignee: Massachusetts Institute of TechnologyInventors: Peter A. Carr, Brian Y. Chow, Joseph M. Jacobson, David W. Mosley, Christopher Emig
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Patent number: 7932061Abstract: This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Specifically, this invention enables the extraction of circulating RNA from plasma or serum and utilizes nucleic acid amplification assays for the identification, detection, inference, monitoring, or evaluation of any neoplasm, benign, premalignant, or malignant, in humans or other animals, which might be associated with that RNA. Further, this invention allows the qualitative or quantitative detection of tumor-derived or associated extracellular RNA circulating in the plasma or serum of humans or animals with or without any prior knowledge of the presence of cancer or premalignant tissue.Type: GrantFiled: February 28, 2006Date of Patent: April 26, 2011Assignee: OncoMEDx, Inc.Inventor: Michael Kopreski
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Publication number: 20110092690Abstract: The invention provides a universal linker capable of synthesizing nucleic acid having a phosphate group at the 3? terminal, a universal support carrying the linker, and a synthesis method of nucleic acid using the universal support. The linker for solid phase synthesis of nucleic acid contains a compound represented by at least one of the following formulae wherein each symbol is as defined in the specification.Type: ApplicationFiled: October 20, 2010Publication date: April 21, 2011Applicants: NITTO DENKO CORPORATION, NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITYInventors: Yoshihiro HAYAKAWA, Masaki TSUKAMOTO, Kenjiro MORI, Kenjiro MINOMI, Eri MAETA, Tatsuya KONISHI
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Patent number: 7928210Abstract: Disclosed herein are novel radiolabeled nucleosides and methods for detecting cellular proliferation in a mammal, the method comprising administrating an effective amount of a radiolabeled nucleoside; the method comprising: a) administering to the mammal a diagnostically effective amount of the nucleoside to the mammal; b) allowing the nucleoside to distribute into the effective tissue; and c) imaging the tissue, wherein an increase in binding of the compound to tissue compared to a normal control level of binding indicates that the mammal is suffering from a disease involving cellular proliferation.Type: GrantFiled: March 3, 2008Date of Patent: April 19, 2011Assignee: Siemens Medical Solutions USA, Inc.Inventors: Hartmuth C. Kolb, Joseph C. Walsh, Robert M Yeh, Kai Chen, Umesh Gangadharmath, Brian Duclos, Vani P. Mocharla, Farhad Karimi, Henry C. Padgett, Qianwa Liang, Tieming Zhao
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Patent number: 7928211Abstract: The present invention provides compositions, compounds and methods for in vitro DNA display technology, allowing display of a variety of molecules, in particular molecules obtained by water incompatible mechanisms. Advantages of such methods are that combinatorial libraries can be constructed comprising molecules which are obtained through water incompatible reactions.Type: GrantFiled: May 3, 2007Date of Patent: April 19, 2011Assignee: Vipergen Pharmaceuticals APSInventors: Nils Jakob Vest Hansen, Peter Blakskjaer, Lars Kolster Petersen
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Patent number: 7927804Abstract: Provided are a method of forming a signal probe-polymer which makes it possible to form a polymer efficiently and quantitatively, a signal probe-polymer formed by the method, oligonucleotide probes for use in the method, and a method of detecting target analyte having high sensitivity and excellent quantitative capability. The method of forming a signal probe-polymer comprises reacting a plurality of pairs of oligonucleotide probes with each other to form a polymer, a first probe of the pair of oligonucleotide probes comprising three nucleic acid regions of X, Y, and Z regions, located in the stated order from the 5?-terminal and a second probe comprising three nucleic acid regions of X?, Y?, and Z? regions, located in the stated order from the 5?-terminal, wherein each region of the oligonucleotide probes has a length of from 13 to 15 bases.Type: GrantFiled: March 14, 2007Date of Patent: April 19, 2011Assignee: Eisai & Managment Co., Ltd.Inventors: Chikako Hakii, Mitsugu Usui
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Patent number: 7923550Abstract: The present invention describes novel compounds and methods for capping reactive groups on support and during multistep synthesis. These new capping reagents are also useful for high quality synthesis on solid supports and surfaces used as microarrays, biosensors, or in general as biochips. The compounds are also useful for controlling surface density of reactive groups on a support. The compounds may also be used to modify the hydrophilic/hydrophobic characteristics of a surface or a molecule. The compounds have functional utility in various applications in the fields of genomics, proteomics, diagnostics and medicine.Type: GrantFiled: April 14, 2004Date of Patent: April 12, 2011Inventors: Xiaolian Gao, Peilin Yu
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Publication number: 20110077389Abstract: Novel CE-phosphoramidites and CPG reagents have been synthesized from a serinol backbone. These reagents are useful to introduce functional groups or directly label oligonucleotides. The versatile serinol scaffold allows for labeling at any position (5? or 3? termini, or any internal position) during automated DNA synthesis. Multiple labels or functional groups can be achieved by repetitive coupling cycles. Optimal spacer arms and protected label moieties have been specially designed. Further, the natural 3-carbon atom internucleotide phosphate distance is retained when inserted internally.Type: ApplicationFiled: September 28, 2010Publication date: March 31, 2011Applicants: Nelson Biotechnologies, Inc., Glen Research CorporationInventors: Paul S. Nelson, Hugh Mackie, Andrew Murphy
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Patent number: 7914991Abstract: The invention relates to a method for isolating and/or identifying known or unknown, nucleic acid sequences (target sequences) which are marked, optionally, with reporter groups, by base specific hybridisation with, essentially, complementary sequences, which belong to a library or sequences. The sample sequences are characterised in that they support, on one of the termini thereof (5?-ends or 3?-ends, preferably on 5?-ends), a single double or multi-chained lipid part, which spreads in a monomolecular layer on a liquid gas or liquid-liquid phase boundary layer. The invention also relates to a system for detecting or isolating nucleic acids.Type: GrantFiled: June 22, 2006Date of Patent: March 29, 2011Inventor: Helmut Rosemeyer
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Patent number: 7914989Abstract: A unimolecular probe for hybridization to a molecule comprising a target nucleic acid sequence, the probe includes: a first nucleic acid sequence complementary to the target sequence (target-binding sequence); and a second nucleic acid sequence complementary to a portion of the first nucleic acid sequence and capable of hybridization therewith to form a first intramolecular duplex. In use, the target and target-bind sequence hybridize to form a duplex. A probe can be used to detect a molecule containing the target sequence, act as a primer for synthesis or amplification, etc.Type: GrantFiled: May 25, 2007Date of Patent: March 29, 2011Assignee: Luminex Molecular Diagnostics, Inc.Inventors: Susan Bortolin, Roman L. Zastawny
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Patent number: 7910312Abstract: Protective groups which may be cleaved with an activatable deprotecting reagents are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.Type: GrantFiled: July 16, 2010Date of Patent: March 22, 2011Assignee: Affymetrix, Inc.Inventors: Robert G. Kuimelis, Glenn H. McGall, Martin J. Goldberg, Guangyu Xu
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Patent number: 7910336Abstract: This invention relates to methods of detecting extracellular hTR RNA and hTERT RNA in blood, plasma, serum, and other bodily fluids. The invention thereby provides an aid for the detection, diagnosis, monitoring, treatment, or evaluation of neoplastic disease.Type: GrantFiled: June 9, 2003Date of Patent: March 22, 2011Assignee: OncoMEDx, Inc.Inventors: Michael S. Kopreski, Christopher D. Gocke
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Publication number: 20110065909Abstract: A method for preparing an oligonucleotide comprising the steps of a) providing a hydroxyl containing compound having the formula (A), b) reacting said compound with a phosphitylating agent in the presence of an activator (activator I) having the formula (I) to prepare a phosphitylated compound; and c) reacting said phosphitylated compound without isolation with a second compound having the formula (A) wherein R5, R3, R2, B are independently selected, but have the same definition as above in the presence of an activator II different from activator I.Type: ApplicationFiled: July 14, 2010Publication date: March 17, 2011Applicant: Girindus AGInventors: Meinolf Lange, Andreas Schönberger, Andreas Hohlfeld, Olaf Grössel, Christina Kirchhoff, Fritz Link
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Patent number: 7902345Abstract: The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5? to 3? nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.Type: GrantFiled: June 4, 2008Date of Patent: March 8, 2011Assignee: Sequenom, Inc.Inventors: Dirk Johannes Van Den Boom, Paul Andrew Oeth, Payam Mahboubi
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Patent number: 7897336Abstract: The present invention describes a method for identification and labeling of sentinel lymph nodes (SLNs) and the presence or absence of lymph node metastases as an important diagnostic and prognostic factor in early stage cancers of all types. The method, know as Molecular Lymphatic Mapping, uses traditional dye/radioactive tracer based techniques in conjunction with a nucleic acid marker to identify and label the SLN, not only for current diagnostic methods, but for archival purposes. In addition, MLM can be used to deliver a therapeutic gene or genes to the SLN to activate tumor immunity to tumor cells, and/or to inhibit tumor metastases. The methods may be combined with therapeutic intervention including chemotherapy and radiotherapy.Type: GrantFiled: August 15, 2003Date of Patent: March 1, 2011Assignee: John Wayne Cancer InstituteInventors: Dave S. B. Hoon, Bret Taback
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Patent number: 7897338Abstract: The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.Type: GrantFiled: July 11, 2006Date of Patent: March 1, 2011Assignee: Applied Biosystems, LLCInventors: Sam Lee Woo, Ronald J. Graham, Jing Tian
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Patent number: 7897736Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.Type: GrantFiled: January 6, 2010Date of Patent: March 1, 2011Assignee: Elitech Holding B.V.Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M. J. Vermeulen
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Patent number: 7897758Abstract: The present invention discloses novel methods for the synthesis of oligonucleotides and nucleoside phosphoramidites. The methods are based on employing aryl-substituted 5-phenyl-1H-tetrazoles with perfluoroalkyl groups on the aromatic ring as activators. In one aspect the novel activators are used in the synthesis of oligonucleotides via the phosphoramidite approach. In this aspect the activators are highly efficient and can be applied with very short coupling times. In a further aspect, the activators of the invention are used in the synthesis of phosphoramidites through the reaction of nucleosides comprising a free hydroxyl group with phosphitylating agents. In this aspect the activators provide very pure phosphoramidites under mild conditions. The activators of the invention are further characterized by being highly soluble, non-hygroscopic and non-hazardous.Type: GrantFiled: April 26, 2006Date of Patent: March 1, 2011Assignee: Sigma-Aldrich Co.Inventors: Andreas Wolter, Michael Leuck
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Patent number: 7892737Abstract: This invention is related to methods, kits and compositions pertaining to stability modulation of PNA oligomer/nucleic acid complexes.Type: GrantFiled: June 29, 2006Date of Patent: February 22, 2011Assignee: Life Technologies CorporationInventors: Subhakar Dey, Eric G. Anderson