The Xanthene Ring System Is Three Cyclos Of A Polycyclo Ring System Having At Least Four Rings (e.g., Benz(c)fluorans, Etc.) Patents (Class 549/224)
  • Patent number: 9250246
    Abstract: Embodiments of near-infrared (NIR) dyes are disclosed, along with methods and kits for detecting analytes with the NIR dyes. The NIR dyes have a structure according to the general structure At least one of R1/R2, R2/R3, R3/R4, R5/R6, R6/R7, and/or R7/R8 together forms a substituted or unsubstituted cycloalkyl or aryl.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: February 2, 2016
    Assignee: Portland State University
    Inventors: Robert Michael Strongin, Martha Sibrian-Vazquez, Jorge Omar Escobedo-Cordova, Mark Allen Lowry
  • Patent number: 9051597
    Abstract: The object of the invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme and compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which includes the compound.
    Type: Grant
    Filed: September 18, 2013
    Date of Patent: June 9, 2015
    Assignees: Mitsubishi Rayon Co., Ltd., The University of Tokyo
    Inventors: Yasuteru Urano, Tetsuo Nagano, Tomoe Ohta, Fujio Yu
  • Publication number: 20150038569
    Abstract: Cervical cancer is one of the most common malignancies and is associated with a dismal prognosis. The most common therapeutic option for cervical cancer consists of surgery in early stages, and chemotherapy or radiotherapy in more advanced stages of the disease. Although treatment options have increased for some patients, overall progress has been modest. This invention relates to a method for treating cancer comprising administering an anti-cancer compound. More particularly, the present invention relates to an anti-cervical-cancer compound for treating human cervical cancer and tumor.
    Type: Application
    Filed: July 31, 2014
    Publication date: February 5, 2015
    Inventors: Hongxi XU, Hong ZHANG, Yuanzhi LAO, Xiaoyu WANG, Kaixian CHEN, Dajian YANG, Shilin CHEN, Chengyuan LIN, Zhaoxiang BIAN, Aiping LU, Albert Sun Chi CHAN
  • Publication number: 20150010938
    Abstract: Probes for selectively detecting compounds comprising a thiol group and an amino group (“thiols”) are disclosed. Exemplary thiols include cysteine, homocysteine, and glutathione. Embodiments of the disclosed probes produce a detectable change in absorbance spectrum and/or emission spectrum when reacted with one or more thiols in solution. Methods and kits for performing the detection also are disclosed. The probes have a general formula where each bond depicted as “” is a single or double bond; R1, R3-R6 and R8 independently are hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen; R2 is an ?,?-unsaturated aliphatic ester; R7 is oxygen, sulfur, hydrogen, hydroxyl, thiol, lower alkyl, carboxyalkyl, amino, alkoxy, or halogen, or R7 and R8 together form a cycloalkyl or aryl ring; X1 is CH2, S, NH, O, Se, Si(CH3)2, Ge(CH3)2, Sn(CH3)2, or C(CH3)2; and X2 is CH, CH2, N, NH, or CR9 where R9 is aryl.
    Type: Application
    Filed: February 22, 2013
    Publication date: January 8, 2015
    Applicant: Oregon State Board of Higher Education on Behalf of Portland State University
    Inventors: Robert M. Strongin, Yixing Guo, Lovemore Hakuna, Mark Allen Lowry, Jorge Omar Escobedo Córdova
  • Patent number: 8906416
    Abstract: The invention relates to compound of generic formula (I) in which: R1 and R2 represent: OH, a hydrogen atom, a C1-C6 alkyl radical a C1-C6 alkoxy radical, a halogen, or OCOR3; R3 represents: a C1-C24 alkyl radical a C12-C24 alkenyl radical comprising at least one unsaturation; R4 represents: COR5, a glucide substituted or not by one or more acetyl radical(s); R5 represents: a C10-C24 alkyl radical or a C12-C24 alkenyl radical comprising at least one unsaturation; R6 and R7 represent:—simultaneously a hydrogen atom or a methyl radical, or—when R6 represents a hydrogen atom, R7 represents a C1-C6 alkyl radical or a phenyl substituted or not by one or more C1-C3 alkoxy radical(s) or one or more halogen(s) or—R6 and R7 are bonded together and form a C3-C6 cycloalkyl, and pharmaceutically or cosmetically acceptable salts.
    Type: Grant
    Filed: July 8, 2011
    Date of Patent: December 9, 2014
    Assignee: Pierre Fabre Dermo-Cosmetique
    Inventors: Stéphane Poigny, Françoise Belaubre
  • Publication number: 20140299851
    Abstract: An organic electroluminescence element that uses a compound expressed by the following general formula has low inter-molecular interaction and high orientation during vapor deposition, and by using compounds that are resistant to aggregation, luminous efficiency is high, and there is little change in chromaticity accompanying drive deterioration (either V1 or W1 and either V2 or W2 form rings, the rings formed by V1 and V2 are six-membered rings, and the rings formed by W1 and W2 are five-membered rings; A2 to A4 and A6 to A8 represent CRZ or N, and RZ represents a hydrogen atom or a substituent; and R2, R3, R7, and R8 represent a hydrogen atom, an alkyl group, an aryl group, a heteroaryl group, a fluorine atom, a hydrogen atom [sic], or a deuterium atom.).
    Type: Application
    Filed: April 11, 2012
    Publication date: October 9, 2014
    Applicant: UDC Ireland Limited
    Inventors: Koji Takaku, Tetsu Kitamura, Toru Watanabe, Yasunori Yonekuta
  • Publication number: 20140120628
    Abstract: Embodiments of near-infrared (NIR) dyes are disclosed, along with methods and kits for detecting analytes with the NIR dyes. The NIR dyes have a structure according to the general structure At least one of R1/R2, R2/R3, R3/R4, R5/R6, R6/R7, and/or R7/R8 together forms a substituted or unsubstituted cycloalkyl or aryl.
    Type: Application
    Filed: June 29, 2012
    Publication date: May 1, 2014
    Applicant: The Oregon State Board of Higher Education on Behalf of Portland State University
    Inventors: Robert Michael Strongin, Martha Sibrian-Vazquez, Jorge Omar Escobedo-Cordova, Mark Allen Lowry
  • Patent number: 8569516
    Abstract: Compounds useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: February 17, 2006
    Date of Patent: October 29, 2013
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • Publication number: 20130252268
    Abstract: The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues.
    Type: Application
    Filed: May 14, 2013
    Publication date: September 26, 2013
    Applicant: Marker Gene Technologies, Inc.
    Inventors: John J. Naleway, Daniel J. Coleman
  • Publication number: 20130130402
    Abstract: Low pKa fluorescent compounds, compositions that include the compounds, bioconjugates made from the compounds, and methods for making and using the compounds and bioconjugates.
    Type: Application
    Filed: May 22, 2012
    Publication date: May 23, 2013
    Applicant: BLOOD CELL STORAGE, INC.
    Inventors: Michael W. Reed, Robert O. Dempcy
  • Patent number: 8389745
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: August 29, 2011
    Date of Patent: March 5, 2013
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Publication number: 20130039904
    Abstract: The present invention discloses a gamboge acid cyclization analogs, their preparation methods and applications by semi-synthesis with the following structural formula I-III: Where ring A, ring B or/and ring C is 4-10 membered saturated or/and unsaturated aliphatic ring aliphatic heterocycle or aryl heterocycle. R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11 or/and R12 is, independently at each occurrence, optionally substituted substituent of glycosyl, multi-hydroxyl, amino acid, acyloxy, phosphoric acid oxy, sulfonyloxy, alkoxy, aryloxy, heterocyclic oxy, thiol, aliphatic or cyclic group containing oxygen, sulfur, nitrogen or phosphorus, one of the substituents or combinations thereof. The present invention has antitumor activity management, antiviral, antibacterial and antifungal activity management, as anti-tumor, anti-viral, immune, antibacterial and antifungal agents, with other known anti-tumor, anti-viral, immune, together with the application of antibacterial and antifungal.
    Type: Application
    Filed: August 2, 2010
    Publication date: February 14, 2013
    Applicant: Liaoning Lifeng Scientific & Technology Development Company Ltd.
    Inventor: Lifeng Xu
  • Publication number: 20120205628
    Abstract: A compound of Formula (I): wherein X, A, Y, Z, R1, R2, Ar, n and m are as described herein. The compound of Formula (I) is useful as part of a semiconducting composition to be deposited upon a surface. When heated, the compound of Formula (I) is converted to a crystalline semiconductor with high mobility.
    Type: Application
    Filed: February 10, 2011
    Publication date: August 16, 2012
    Applicant: XEROX CORPORATION
    Inventors: Yiliang Wu, Anthony James Wigglesworth, Ping Liu, Nan-Xing Hu
  • Patent number: 8134017
    Abstract: A compound represented by the following formula (1) or (2):
    Type: Grant
    Filed: December 2, 2010
    Date of Patent: March 13, 2012
    Assignee: Riken
    Inventors: Shinichiro Kamino, Shuichi Enomoto
  • Patent number: 7982056
    Abstract: The present invention is directed to an improved process for producing substantially pure fluorescein, as well as to substantially pure fluorescein compositions prepared by the process. The invention is particularly directed to the provision of pharmaceutical compositions for use in angiography. The substantially pure fluorescein produced by the process of the present invention is low in color, low in sodium chloride content, and substantially free of pyridine.
    Type: Grant
    Filed: December 4, 2007
    Date of Patent: July 19, 2011
    Assignee: Alcon Research, Ltd.
    Inventors: Gregory Bydlinski, Robert Gregg Harris, Britt S. Scott
  • Patent number: 7902359
    Abstract: This invention relates to a compound of the following formula: wherein R1, R2, R3, G, and Z are defined herein. It also relates to is methods of treating autoimmune disease, cancer, and atherosclerosis with such a compound.
    Type: Grant
    Filed: December 11, 2007
    Date of Patent: March 8, 2011
    Assignee: Hutchison MediPharma Enterprises Limited
    Inventors: Tao Wang, Weihan Zhang, Xiaoqiang Yan, Weiguo Su, Jifeng Duan, Yu Cai
  • Patent number: 7767834
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: August 3, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Publication number: 20100137421
    Abstract: Provided herein are compounds that are inducers of apoptosis activators of caspases and pharmaceutically acceptable derivatives thereof. Also provided are methods of synthesis of the compounds and methods for treatment of diseases in which there is uncontrolled cell growth and spread of abnormal cells, such as cancers, by administering the compounds.
    Type: Application
    Filed: November 8, 2007
    Publication date: June 3, 2010
    Inventors: Emmanuel Theodorakis, Ayse Batova
  • Patent number: 7671218
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: August 12, 2005
    Date of Patent: March 2, 2010
    Assignee: Elitech Holdings B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Patent number: 7642362
    Abstract: Compounds represented by the general formula (1) wherein R1 and R3 are each independently hydrogen or a carboxyl group and R2 and R4 are each independently hydrogen or a hydroxyl group, or pharmaceutically acceptable salts thereof, which exhibit semaphorin-inhibitory activity and are useful as preventives or remedies for neuropathic and neurodegenerative diseases are provided.
    Type: Grant
    Filed: January 22, 2003
    Date of Patent: January 5, 2010
    Assignee: Dainippon Sumitomo Pharma Co., Ltd.
    Inventors: Kazuo Kumagai, Nobuo Hosotani
  • Publication number: 20090317914
    Abstract: A compound represented by the following general formula (I): [R1 represents hydrogen atom, or 1 to 4 monovalent substituents; R2, R4, R5, and R7 represent hydrogen atom, or a monovalent substituent; R3 represents ?O or ?N+(R8)(R9).M? (R8 and R9 represent an alkyl group, and M? represents a counter ion); R6 represents —OH or —N(R10)(R11) (R10 and R11 represent an alkyl group); n represents an integer of 1 to 3; and X represents —SH or —OH], which is useful for selective measurement of hypochlorite ion and the like.
    Type: Application
    Filed: March 1, 2007
    Publication date: December 24, 2009
    Applicant: THE UNIVERSITY OF TOKYO
    Inventors: Tetsuo Nagano, Yasuteru Urano, Suguru Kenmoku
  • Patent number: 7615377
    Abstract: The present invention is directed, in part, to fluorescein-based ligands for detection of metal ions, and methods of making and using the same.
    Type: Grant
    Filed: August 27, 2004
    Date of Patent: November 10, 2009
    Assignee: Massachusetts Institute of Technology
    Inventors: Stephen J. Lippard, Elizabeth Marie Nolan
  • Patent number: 7601851
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: May 10, 2005
    Date of Patent: October 13, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • Patent number: 7541454
    Abstract: Compounds useful in the fluorescent labeling of biological materials are provided along with methods for their use and preparation.
    Type: Grant
    Filed: October 25, 2004
    Date of Patent: June 2, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventor: Eugeny A. Lukhtanov
  • Patent number: 7524974
    Abstract: A fluorescent probe which is represented by the following formula (I): (wherein, R1 and R2 represent hydrogen atom, or a substituent for trapping proton, a metal ion, or an active oxygen species, or the like; R3 represents a monovalent substituent; R4 and R5 represent hydrogen atom or a halogen atom; R6 represents hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, provided that a combination of R1, R2, and R3 provides (1) substantially high electron density of the benzene ring to which said groups bind, so that the compound represented by the formula (I) is substantially no fluorescent before the trapping of proton, or the like, and (2) substantially reduced electron density of the benzene ring to which said groups bind, so that a compound after the trapping, which is derived from the compound represented by the formula (I) is substantially highly fluorescent after the trapping of proton or the like). A fluorescent probe having an excellent fluorescence property is provided.
    Type: Grant
    Filed: July 7, 2003
    Date of Patent: April 28, 2009
    Assignees: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Tetsuo Nagano, Yasuteru Urano, Suguru Kenmoku, Kohjiro Kanda
  • Patent number: 7408072
    Abstract: The present invention provides heteropolycyclic compounds represented by General Formulae (1) and (2): wherein R1 is a straight- or branched-chain C1-C10 alkyl group or the like; R2 and R3 are the same or different and are each a straight- or branched-chain C1-C10 alkyl group or the like; R4 and R5 are each a hydrogen atom; X is —OH or the like; X? is a straight- or branched-chain C1-C10 alkyl group or the like; and Z is —O— or the like. The heteropolycyclic compounds of the present invention have appropriate optical absorption wavelengths and fluorescence wavelengths and a high luminescence intensity, and can be advantageously used in various applications as colorants that have excellent performance in terms of heat resistance, light resistance, solubility, dispersibility in resist materials, solid luminescence, etc.
    Type: Grant
    Filed: February 10, 2005
    Date of Patent: August 5, 2008
    Assignee: Kochi University
    Inventors: Katsuhira Yoshida, Yousuke Ooyama
  • Patent number: 7399854
    Abstract: Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
    Type: Grant
    Filed: December 28, 2006
    Date of Patent: July 15, 2008
    Assignee: Applera Corporation
    Inventors: Linda G. Lee, Sandra L. Spurgeon, Barnett Rosenblum
  • Patent number: 7179906
    Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1–R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof, and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having the general structure where substituents R3–R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates.
    Type: Grant
    Filed: September 5, 2003
    Date of Patent: February 20, 2007
    Assignee: Applera Corporation
    Inventors: Scott C. Benson, Steven M. Menchen, Peter D. Theisen, Kevin M. Hennessey, Vergine C. Furniss, Joan D. Hauser
  • Patent number: 7074823
    Abstract: A compound represented by general formula (IA) or a salt thereof useful as a fluorescent probe for zinc: wherein R1 and R2 represent a hydrogen atom or a group represented by formula (A), wherein X1, X2, X3, and X4 represent a hydrogen atom, an alkyl group, a 2-pyridylmethyl group, or a protective group for an amino group, and m and n represent 0 or 1, provided that R1 and R2 do not simultaneously represent hydrogen atoms; R3 and R4 represent a hydrogen atom or a halogen atom; and R5 and R6 represent a hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, and R7 represents a hydrogen atom or an alkyl group
    Type: Grant
    Filed: November 23, 2004
    Date of Patent: July 11, 2006
    Assignees: Daiichi Pure Chemical Co., Ltd.
    Inventors: Tetsuo Nagano, Kazuya Kikuchi, Tomoya Hirano
  • Patent number: 7038063
    Abstract: Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
    Type: Grant
    Filed: November 18, 2003
    Date of Patent: May 2, 2006
    Assignee: Applera Corporation
    Inventors: Linda G. Lee, Meng C. Taing, Barnett B. Rosenblum
  • Patent number: 6967250
    Abstract: Energy transfer dyes, their preparation, and their use as labels in biological systems is disclosed. The dyes are preferably in the form of cassettes which enable their attachment to a variety of biological materials. The dyes and the reagents that can be made from them offer a wide variety of fluorescent labels with large Stokes' shifts enabling their use in a variety of fluorescence applications over a wide range of the visible spectrum.
    Type: Grant
    Filed: August 30, 1999
    Date of Patent: November 22, 2005
    Assignee: Amersham Biosciences Corp
    Inventors: Shiv Kumar, Satyam Nampalli, Mahesh Khot
  • Patent number: 6916925
    Abstract: Dye labeled imidazonaphthyridine, imidazopyridine and imidazoquinoline compounds having immune response modulating activity are disclosed. The compounds arc useful, inter alia, for determining the binding and/or receptor sites of the molecules.
    Type: Grant
    Filed: July 22, 2003
    Date of Patent: July 12, 2005
    Assignee: 3M Innovative Properties Co.
    Inventors: Michael J. Rice, Mark A. Tomai, Ai-Ping Wei
  • Patent number: 6833386
    Abstract: A compound represented by the following formula (I): wherein R1 and R2 represent amino groups that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group; and R3 and R4 independently represent hydrogen atom or an acyl group, and an agent for measurement of nitrogen monoxide which comprises said compound.
    Type: Grant
    Filed: April 14, 2003
    Date of Patent: December 21, 2004
    Assignees: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Tetsuo Nagano, Hirotatsu Kojima
  • Publication number: 20040225119
    Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure 1
    Type: Application
    Filed: September 5, 2003
    Publication date: November 11, 2004
    Applicant: Applera Corporation
    Inventors: Scott C. Benson, Steven M. Menchen, Peter D. Theisen, Kevin M. Hennessey, Vergine C. Furniss, Joan D. Hauser
  • Patent number: 6649769
    Abstract: Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
    Type: Grant
    Filed: August 21, 2002
    Date of Patent: November 18, 2003
    Assignee: Applera Corporation
    Inventors: Linda G. Lee, Meng C. Taing, Barnett B. Rosenblum
  • Patent number: 6635602
    Abstract: A process for the manufacture of mixtures of fluoran compounds by the reaction of keto acids of formula (II) with a compound of formula (III) in the presence of a dehydrating condensation agent, wherein R1 and R2 independently represent hydrogen; an alkyl of 1-18 carbon, a secondary alkyl with respect to the carbon atom bonded to the nitrogen atom of 3-13 carbon atoms, a cycloalkyl of 4-8 carbon atoms or a phenyl, both of which may be substituted by at least one substituted selected from the group consisting of halogen atoms and alkyls having 1-4 carbon atoms, an aralkyl of 7-10 carbon atoms; or R1 and R2, together with the adjacent nitrogen atom from a heterocyclic ring; R3 is hydrogen, an alkyl of 1-4 carbon atoms, an alkoxy of 1-4 carbon atoms, a phenyl; a substituted phenyl or a halogen; R4 is an alkyl group of 1-18 carbon atoms, a carboxyalkyl of 1-18 carbon atoms, a carboxycycloalkyl of 4-8 carbon atoms, an alkylamino of 1-18 carbon atoms, a cycloalkylamino of 4-8 carbon atoms, a dialkylamino or dicyclo
    Type: Grant
    Filed: February 26, 2001
    Date of Patent: October 21, 2003
    Assignee: Ciba Specialty Chemicals Corporation
    Inventors: James Philip Taylor, Michael Heneghan
  • Patent number: 6632933
    Abstract: The use of azetidinone compounds that are inhibitors of cholesterol absorption as tools for discovering and characterizing proteins involved in trafficking or absorption of cholesterol and/or cholesteryl esters in biological systems is presented. These compounds can serve as tools for competitive binding assays to discover and characterize other chemical agents useful as cholesterol absorption inhibitors. New compounds of the present invention are also highly efficacious inhibitors of cholesterol absorption.
    Type: Grant
    Filed: July 30, 2001
    Date of Patent: October 14, 2003
    Assignee: Schering Corporation
    Inventors: Scott W. Altmann, Duane A. Burnett, Harry R. Davis, Jr., Michael P. Graziano, Maureen Laverty, Xiaorui Yao
  • Patent number: 6630588
    Abstract: Dye labeled imidazonaphthyridine, imidazopyridine and imidazoquinoline compounds having immune response modulating activity are disclosed. The compounds are useful, inter alia, for determining the binding and/or receptor sites of the molecules.
    Type: Grant
    Filed: February 19, 2002
    Date of Patent: October 7, 2003
    Assignee: 3M Innovative Properties Company
    Inventors: Michael J. Rice, Mark A. Tomai, Ai-Ping Wei
  • Patent number: 6617445
    Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1-R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having the general structure where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect the invention includes methods for synthesizing the above dye compounds and intermediates.
    Type: Grant
    Filed: October 11, 2001
    Date of Patent: September 9, 2003
    Assignee: Applera Corporation
    Inventors: Scott C. Benson, Steven M. Menchen, Peter D. Theisen, Kevin M. Hennessey, Vergine C. Furniss, Joan Hauser
  • Patent number: 6608213
    Abstract: This invention provides a precursor compound of a novel fluorescence-labeled probe for DNA, prepared by covalent binding of a fluorescent substance such as fluorescein isothiocyanate, wherein a conjugating group was bounded, with aminoalkylate phenylamine. This invention also provides a novel fluorescence-labeled probe for DNA by diazotizing amino group of phenylamine contained in the precursor compound. Furthermore, a fluorescence labeled plasmid can be produced by conjugating the fluorescence-labeled probe and purine ring of guanine contained in the DNA plasmid through diazonium group.
    Type: Grant
    Filed: March 22, 2001
    Date of Patent: August 19, 2003
    Assignee: Tokyo Institute of Technology
    Inventors: Toshinori Sato, Yoshio Okahata
  • Patent number: 6569892
    Abstract: A compound represented by the following formula: wherein R1 and R2 represent amino groups that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group, and wherein R3 represents hydrogen atom or an alkyl group and R4 represents hydrogen atom or an acyl group, and an agent for measurement of nitrogen monoxide which comprises said compound.
    Type: Grant
    Filed: May 8, 2002
    Date of Patent: May 27, 2003
    Assignees: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Tetsuo Nagano, Hirotatsu Kojima
  • Patent number: 6566302
    Abstract: A monophase solid solution comprising a plurality of color formers selected from the group consisting essentially of the fluoran type, phthalide type, phenoxazine type, phenothiazine type, rhodamine lactam type, leuco-auramine type, triphenylmethane type, spiropyran type, benzoxazine type, quinazoline type of color formers and mixtures thereof, preferably wherein the color forming materials are selected from the fluoran and phthalide type of color former and mixtures thereof.
    Type: Grant
    Filed: February 26, 2001
    Date of Patent: May 20, 2003
    Assignee: Ciba Specialty Chemicals Corporation
    Inventors: James Philip Taylor, Michael Heneghan
  • Patent number: 6482938
    Abstract: Disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, for example, fluorescent groups, and having a 2′, 3′-dideoxyribonucleotide residue or a 3′-deoxyribonucleotide residue. These compounds can be used as terminators for the chain terminator method. The two kinds of reporters are arranged with a distance sufficient for causing energy transfer from the donor to the acceptor. Also disclosed are methods for determining DNA sequences based on the chain terminator method wherein the chain termination reaction is performed by using the above terminators. Also disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, which can be used as a primer or an initiator in methods for determining DNA sequences utilizing the chain terminator method, and methods for determining DNA sequences utilizing the compounds.
    Type: Grant
    Filed: September 17, 1999
    Date of Patent: November 19, 2002
    Assignees: Wako Pure Chemical Industries, Ltd., The Institute of Physical & Chemical Research
    Inventors: Yoshihide Hayashizaki, Takumi Tanaka
  • Patent number: 6448407
    Abstract: Substantially pure atropisomers of xanthene compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.
    Type: Grant
    Filed: November 1, 2000
    Date of Patent: September 10, 2002
    Assignee: PE Corporation (NY)
    Inventors: Linda G. Lee, Meng C. Taing, Barnett B. Rosenblum
  • Patent number: 6441197
    Abstract: A compound represented by the following formula (I): wherein R1 and R2 represent amino group that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group; and R3 and R4 independently represent hydrogen atom or an acyl group, and an agent for measurement of nitrogen monoxide which comprises said compound.
    Type: Grant
    Filed: January 20, 2000
    Date of Patent: August 27, 2002
    Assignees: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Tetsuo Nagano, Hirotatsu Kojima
  • Publication number: 20020115067
    Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure 1
    Type: Application
    Filed: October 11, 2001
    Publication date: August 22, 2002
    Applicant: The Perkin-Elmer Corporation
    Inventors: Scott C. Benson, Steven M. Menchen, Peter D. Theisen, Kevin M. Hennessey, Vergine C. Furniss, Joan D. Hauser
  • Patent number: 6403812
    Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dicloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
    Type: Grant
    Filed: May 30, 2000
    Date of Patent: June 11, 2002
    Assignee: The Perkin-Elmer Corporation
    Inventors: Steven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
  • Patent number: 6376669
    Abstract: Dye labeled imidazonaphthyridine, imidazopyridine and imidazoquinoline compounds having immune response modulating activity are disclosed. The compounds are useful, inter alia, for determining the binding and/or receptor sites of the molecules.
    Type: Grant
    Filed: November 2, 2000
    Date of Patent: April 23, 2002
    Assignee: 3M Innovative Properties Company
    Inventors: Michael J. Rice, Mark A. Tomai, Ai-Ping Wei
  • Patent number: 6346536
    Abstract: Poly(ADP-ribose) polymerase (“PARP”) inhibitors and methods for treating neurodegenerative diseases, neural tissue damage related to cerebral ischemia and reperfusion injury, and cardiovascular in an animal.
    Type: Grant
    Filed: September 3, 1997
    Date of Patent: February 12, 2002
    Assignee: Guilford Pharmaceuticals Inc.
    Inventors: Jia-He Li, Paul F. Jackson, Keith M. Maclin, Jie Zhang
  • Patent number: 6303775
    Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure wherein Y1 and Y2 are individually hydroxyl, amino, imminium, or oxygen, R1-R8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof, and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having the general structure where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates.
    Type: Grant
    Filed: February 1, 2000
    Date of Patent: October 16, 2001
    Assignee: The Perkin-Elmer Corporation
    Inventors: Scott C. Benson, Steven M. Menchen, Peter D. Theisen, Kevin M. Hennessey, Vergine C. Furniss, Joan Hauser