Xanthene Ring System Having A Benzene Ring Bonded Directly Thereto Para To The Xanthene Ring Oxygen, Wherein The Carbon Of A -coo- Group Is Bonded Directly To The Benzene Ring Ortho To Its Position Of Attachment To The Xanthene Ring System, And Spirolactone Derivatives Thereof (e.g., Fluoresceins, Chlorofluorans, Etc.) Patents (Class 549/223)
  • Patent number: 10526646
    Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.
    Type: Grant
    Filed: December 19, 2016
    Date of Patent: January 7, 2020
    Assignee: Agilent Technologies, Inc.
    Inventors: Jesper Lohse, Mike Paw Maischnack Hansen
  • Patent number: 9771480
    Abstract: This invention is directed to photoluminescent compounds based on rhodamine dyes with red-shifted absorption and emission maxima and uses thereof for photoluminescence based devices.
    Type: Grant
    Filed: August 31, 2016
    Date of Patent: September 26, 2017
    Assignee: StoreDot Ltd.
    Inventors: Evgenia Liel (Jeny) Kuks, Rony Schwarz, Eran Sella, Mor Shmuel Armon, Daniel Szwarcman
  • Patent number: 9273022
    Abstract: A new process for the manufacture of iodinated xanthenes in high purity includes a cyclization step followed by an iodination step. No extraction, chromatographic or solvent concentration steps are required, and the intermediate as well as final compounds are isolated via filtration or similar means. The process requires a single organic solvent, and the steps are completed at temperatures below 100° C. The exclusion of chloride ions, of chloride free-radicals, hypochlorite ions, or hypochlorous acid as reagents or from reagents that may generate these species in situ in the presence of oxidants, prevents undesirable impurity formation. Several new compounds have been conceived and isolated using these methods. These new compounds are also formed into new medicaments.
    Type: Grant
    Filed: July 30, 2014
    Date of Patent: March 1, 2016
    Assignee: Provectus Pharmaceuticals, Inc.
    Inventors: Jamie Singer, Eric A. Wachter, Timothy Scott, Marlon Lutz, Kevin Babiak, Sahadeva Reddy Damireddi, J. Robert Durrwachter
  • Publication number: 20150148259
    Abstract: Novel phenyl-glyoxal based anti-citrulline probes and methods of synthesis are provided. Methods of use, such as, the development of methods for monitoring substrate citrullination over time; for identifying citrullinated proteins from cells are described.
    Type: Application
    Filed: June 4, 2013
    Publication date: May 28, 2015
    Inventors: Paul R. Thompson, Kevin L. Bicker, Venkataraman Subramanian
  • Publication number: 20150110715
    Abstract: The present invention relates to a compound or a pharmaceutically acceptable salt thereof having a chemical structure comprising: (A) at least one motif specifically binding to cell membranes of neoplastic cells; (B) at least one chelator moiety of radiometals; and (C) at least one dye moiety; wherein said compound has a molecular weight of not more than 5 kDa. Further, the invention refers to a method for producing such compound and to the in vivo and in vitro uses thereof.
    Type: Application
    Filed: July 18, 2014
    Publication date: April 23, 2015
    Applicants: RUPRECHT-KARLS-UNIVERSITAT HEIDELBERG, DEUTSCHES KREBSFORSCHUNGSZENTRUM
    Inventors: Matthias Eder, Klaus Kopka, Martin Schaefer, Ulrike Bauder-Wuest, Uwe Haberkorn
  • Patent number: 8895317
    Abstract: A compound represented by the general formula (I) [R1 and R2 are amino groups that substitute at adjacent positions on the benzene ring; R3 and R4 are halogen atoms; R5 and R6 represent hydrogen atom, an acyl group or an acyloxy(C1-6 alkyl) group; R7 and R8 represent —(CH2)p—N(R9)(R10) (p is 1 to 4, and R9 and R10 represent —(CH2)n—COOH (n is 1 to 4))], which is useful for measuring a reactive nitrogen species existing in cells such as nitrogen monoxide or peroxynitrite at high sensitivity over a long period of time.
    Type: Grant
    Filed: December 4, 2009
    Date of Patent: November 25, 2014
    Assignee: The University of Tokyo
    Inventors: Tetsuo Nagano, Yasuteru Urano, Saki Izumi
  • Publication number: 20140343296
    Abstract: A new process for the manufacture of iodinated xanthenes in high purity includes a cyclization step followed by an iodination step. No extraction, chromatographic or solvent concentration steps are required, and the intermediate as well as final compounds are isolated via filtration or similar means. The process requires a single organic solvent, and the steps are completed at temperatures below 100° C. The exclusion of chloride ions, of chloride free-radicals, hypochlorite ions, or hypochlorous acid as reagents or from reagents that may generate these species in situ in the presence of oxidants, prevents undesirable impurity formation. Several new compounds have been conceived and isolated using these methods. These new compounds are also formed into new medicaments.
    Type: Application
    Filed: July 30, 2014
    Publication date: November 20, 2014
    Inventors: Jamie SINGER, Eric A. WACHTER, Timothy SCOTT, Marlon LUTZ, Kevin BABIAK, Sahadeva Reddy DAMIREDDI, J. Robert DURRWACHTER
  • Publication number: 20140308342
    Abstract: The present invention relates to chimeric (preferably, bifunctional) compounds, compositions comprising those compounds and methods of treating cancer in a patient or subject, especially including metastatic cancer where cancer cells exhibit overexpression (heightened expression) of cell surface urokinase-type plasminogen activator receptor (urokinase receptor) compared to normal (non-cancerous) cells. The compounds preferably covalently bind to the urokinase receptor and recruit native antibodies of the patient or subject where the antibodies can selectively degrade and/or deactivate targeted cancer cells through antibody-dependent cellular phagocytosis and/or antibody-dependent cellular cytotoxicity (ADCC) against a large number and variety of cancers, thus providing cancer cell death and/or an inhibition of growth, elaboration and/or metastasis of the cancer, including remission and cure of the patient's cancer.
    Type: Application
    Filed: November 7, 2012
    Publication date: October 16, 2014
    Inventors: David A. Spiegel, Charles E. Jakobsche
  • Patent number: 8852400
    Abstract: The present disclosure provides for a method of preparing and using an emulsion for treating a papermaking process. The emulsion is an oil-in-water emulsion of alkenyl succinic anhydride emulsified with a polymer comprising at least one primary or secondary amine containing monomer. The method comprises adding an oil-in-water emulsion to the papermaking process; wherein the oil-in-water emulsion comprises alkenyl succinic anhydride emulsified with a polymer comprising at least one primary or secondary amine containing monomer; and wherein the oil-in-water emulsion is added in an amount sufficient to improve sizing of the paper produced by the papermaking process. The primary or secondary amine may be a secondary amine comprising diallylamine, and the polymer may be a diallylamine-acrylamide copolymer.
    Type: Grant
    Filed: November 15, 2012
    Date of Patent: October 7, 2014
    Assignee: Ecolab USA Inc.
    Inventors: Michael R St. John, David J Castro, Mei Liu
  • Publication number: 20140248218
    Abstract: Herein are provided, inter alia, compositions including boronic esters, which in the presence of H2O2, provide for the detection of ROS compounds such as endogenous H2O2 and methods of using the compositions to detect ROS compounds.
    Type: Application
    Filed: February 7, 2014
    Publication date: September 4, 2014
    Applicant: The Regents of the University of California
    Inventors: Kevin B. Daniel, Seth M. Cohen, Jody L. Major Jourden
  • Publication number: 20140239279
    Abstract: The present disclosure relates to an organic electroluminescent compound employed as a hole transport material or a hole injection material and an organic electroluminescent device including the same. The organic electroluminescent compound is represented by [Chemical Formula 1] and an organic electroluminescent device employing the organic electroluminescent compound as a hole transport material exhibits very superior luminous efficiency and lifetime characteristics.
    Type: Application
    Filed: February 28, 2014
    Publication date: August 28, 2014
    Applicant: SFC Co., Ltd.
    Inventors: Sang-woo PARK, Byung-sun Yang, Se-Jin Lee
  • Patent number: 8779165
    Abstract: Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.
    Type: Grant
    Filed: March 2, 2011
    Date of Patent: July 15, 2014
    Assignee: AAT Bioquest, Inc.
    Inventors: Zhenjun Diwu, Jianjun He, Jinfang Liao
  • Patent number: 8697383
    Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.
    Type: Grant
    Filed: May 25, 2011
    Date of Patent: April 15, 2014
    Assignees: Mitsubishi Rayon Co., Ltd., The University of Tokyo
    Inventors: Yasuteru Urano, Tetsuo Nagano, Tomoe Ohta, Fujio Yu
  • Publication number: 20140072990
    Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.
    Type: Application
    Filed: September 18, 2013
    Publication date: March 13, 2014
    Applicants: The University of Tokyo, Mitsubishi Rayon Co., Ltd.
    Inventors: Yasuteru URANO, Tetsuo NAGANO, Tomoe OHTA, Fujio YU
  • Publication number: 20130337536
    Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.
    Type: Application
    Filed: August 21, 2013
    Publication date: December 19, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Evan Antoulinakis, Kyle Gee, Aleksey Rukavishnikov
  • Publication number: 20130315836
    Abstract: Provided herein are compounds useful for selectively labeling an apoptotic cell, and methods and assays using such compounds for the detection of an apoptotic cell in vivo or in a biological sample obtained from a subject.
    Type: Application
    Filed: May 3, 2013
    Publication date: November 28, 2013
    Applicant: TRUSTEES OF BOSTON COLLEGE
    Inventors: Jianmin Gao, Hong Zheng
  • Publication number: 20130274322
    Abstract: A new process for the manufacture of iodinated xanthenes in high purity includes a cyclization step followed by an iodination step. No extraction, chromatographic or solvent concentration steps are required, and the intermediate as well as final compounds are isolated via filtration or similar means. The process requires a single organic solvent, and the steps are completed at temperatures below 100° C. The exclusion of chloride ions, of chloride free-radicals, hypochlorite ions, or hypochlorous acid as reagents or from reagents that may generate these species in situ in the presence of oxidants, prevents undesirable impurity formation. Several new compounds have been conceived and isolated using these methods. These new compounds are also formed into new medicaments.
    Type: Application
    Filed: June 12, 2013
    Publication date: October 17, 2013
    Inventors: Jamie Singer, Eric A. Wachter, Timothy Scott, Marlon Lutz, Kevin Babiak
  • Publication number: 20130252268
    Abstract: The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the organelle and label these organelles, such as lysosomes, rendering them visible and easily observed. Substrates of the present invention include substrates that produce a fluorescent signal. The fluorogenic acidic organelle enzyme substrates of this invention are designed to provide high fluorescence at low pH values and are derivatized to permit membrane permeation through both outer and organelle membranes of intact cells and can be used for staining cells at very low concentrations. They can be used for monitoring enzyme activity in cells at very low concentrations and are not toxic to living cells or tissues.
    Type: Application
    Filed: May 14, 2013
    Publication date: September 26, 2013
    Applicant: Marker Gene Technologies, Inc.
    Inventors: John J. Naleway, Daniel J. Coleman
  • Patent number: 8530675
    Abstract: A new process for the manufacture of iodinated xanthenes in high purity includes a cyclization step followed by an iodination step. No extraction, chromatographic or solvent concentration steps are required, and the intermediate as well as final compounds are isolated via filtration or similar means. The process requires a single organic solvent, and the steps are completed at temperatures below 100° C. The exclusion of chloride ions, of chloride free-radicals, hypochlorite ions, or hypochlorous acid as reagents or from reagents that may generate these species in situ in the presence of oxidants, prevents undesirable impurity formation. Several new compounds have been conceived and isolated using these methods. These new compounds are also formed into new medicaments.
    Type: Grant
    Filed: September 17, 2010
    Date of Patent: September 10, 2013
    Assignee: Provectus Pharmaceuticals, Inc.
    Inventors: Jamie Singer, Eric A. Wachter, Timothy Scott, Marlon Lutz, Kevin Babiak
  • Patent number: 8426447
    Abstract: The present invention comprises a new class of compounds useful for the modulation of Beta-secretase enzyme activity and for the treatment of Beta-secretase mediated diseases, including Alzheimer's disease (AD) and related conditions. In one embodiment, the compounds have a general Formula I wherein A1, A2, A3, A4, A5, A6, T1, T2, W, X, Y and Z of Formula I are defined herein. The invention also includes use of these compounds in pharmaceutical compositions for treatment, prophylactic or therapeutic, of disorders and conditions related to the activity of beta-secretase protein. Such disorders include, for example, Alzheimer's Disease, cognitive deficits, cognitive impairment, schizophrenia and other central nervous system conditions related to and/or caused by the formation and/or deposition of plaque on the brain. The invention also comprises further embodiments of Formula I, intermediates and processes useful for the preparation of compounds of Formula I.
    Type: Grant
    Filed: September 11, 2009
    Date of Patent: April 23, 2013
    Assignee: Amgen Inc.
    Inventors: Ryan White, Albert Amegadzie, Marian C. Bryan, Jian Jeffrey Chen, Alan C. Cheng, Thomas Dineen, Oleg Epstein, Vijay Keshav Gore, Zihao Hua, Jason Brooks Human, Hongbing Huang, Charles Kreiman, Daniel La, Qingyian Liu, Vu Van Ma, Isaac Marx, Vinod F Patel, Wenyuan Qian, Matthew Weiss, Chester Chenguang Yuan
  • Patent number: 8420402
    Abstract: The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
    Type: Grant
    Filed: January 27, 2012
    Date of Patent: April 16, 2013
    Assignee: AnaSpec Incorporated
    Inventors: Jianjun He, Anita Hong, Qingheng Wang, Raman Khosrow Afshar, Ming Zhu, Vera Rakhmanova
  • Publication number: 20130089853
    Abstract: Provided are various compounds comprising the formula Also provided are fluorescent dyes comprising the above compound. Additionally, a fluorescence energy transfer system is provided that comprises the above-described fluorescent dye and a second dye, wherein the second dye is capable of energy transfer with the fluorescent dye. Further provided is a kit for labeling a target molecule, where the kit comprises the above-described fluorescent dye with additional reagents useful for labeling the target molecule. Additionally provided is a target molecule labeled with the above-described fluorescent dye. A method of labeling a target molecule is also provided. The method comprises contacting reactive group Z of the above-described fluorescent dye with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between reactive group Z and the target molecule. Also, another method of labeling a target molecule is provided.
    Type: Application
    Filed: October 11, 2011
    Publication date: April 11, 2013
    Applicant: ENZO LIFE SCIENCES, INC., C/O ENZO BIOCHEM, INC.
    Inventors: ZAIGUO LI, PRAVEEN PANDE
  • Patent number: 8410164
    Abstract: A compound represented by the formula (I) (R1 represents an aryl group such as hydroxyphenyl group, R2 represents 2-carboxyphenyl group etc., and R3 and R4 represent —(CH2)p—N(R5)(R6) (R5 and R6 represent —(CH2)n—COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.
    Type: Grant
    Filed: May 15, 2009
    Date of Patent: April 2, 2013
    Assignee: The University of Tokyo
    Inventors: Tetsuo Nagano, Yasuteru Urano, Saki Izumi
  • Publication number: 20130065263
    Abstract: The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.
    Type: Application
    Filed: May 25, 2011
    Publication date: March 14, 2013
    Applicants: The University of Tokyo, Mitsubishi Rayon Co., Ltd.
    Inventors: Yasuteru Urano, Tetsuo Nagano, Tomoe Ohta, Fujio Yu
  • Patent number: 8389745
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: August 29, 2011
    Date of Patent: March 5, 2013
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Patent number: 8357701
    Abstract: Therapeutic agents for treatment of Galactosemia and disorders thereof, and other enzyme related disorders thereof are identified. The compounds inhibit galactokinase (GALK) and other kinase activity and are identified by a high throughput screening assay.
    Type: Grant
    Filed: August 14, 2008
    Date of Patent: January 22, 2013
    Assignee: University of Miami
    Inventors: Kent Lai, Klass Jan Wierenga, Manshu Tang
  • Publication number: 20120316340
    Abstract: The use of metal-accumulating plants for implementing chemical reactions.
    Type: Application
    Filed: November 18, 2010
    Publication date: December 13, 2012
    Applicants: UNIVERSITE MONTPELLIER 2 SCIENCES ET TECHNIQUES, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Claude Grison, Jose Escarre
  • Publication number: 20120310141
    Abstract: Devices and systems for delivering light to a target are described. Methods of using such light delivery device and system are also described. A method of using a photosensitizing compound with the light delivery device is also described.
    Type: Application
    Filed: May 4, 2012
    Publication date: December 6, 2012
    Inventors: Julia A. KORNFIELD, Matthew S. MATTSON, Joyce HUYNH
  • Publication number: 20120259098
    Abstract: The present invention relates to novel dendrimer compounds and methods of synthesizing the same. In particular, the present invention is directed to novel polyamidoamine (PAMAM) dendrimers, novel dendrimer branching units, methods for synthesizing such novel PAMAM dendrimers and functionalized dendrimers, as well as systems and methods utilizing the dendrimers (e.g., in diagnostic and/or therapeutic settings (e.g., for the delivery of therapeutics, imaging, and/or targeting agents (e.g., in disease diagnosis and/or therapy, etc.))).
    Type: Application
    Filed: October 7, 2010
    Publication date: October 11, 2012
    Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: James R. Baker, JR., Baohua Huang
  • Publication number: 20120220787
    Abstract: A process for the preparation of a mixture of 3?,6?-dihydroxy-6-nitrospiro[2-benzofuran-3,9?-xanthene]-1-one and 3?,6?-dihydroxy-5-nitrospiro[2-benzofuran-3,9?- xanthene]-1-one comprising the steps of:- (a) reacting 4-nitrophthalic acid or 4-nitrophthalic anhydride with benzene-1,3-diol in methanesulphonic acid; (b) quenching the reaction in step (a) with a solvent to precipitate product; (c) isolating the precipitate; (d) heating the precipitate in water in order to hydrolyse any methansulphonic acid ester present.
    Type: Application
    Filed: February 10, 2010
    Publication date: August 30, 2012
    Inventors: Stuart Wharton, Tomos Huw Roberts
  • Publication number: 20120184683
    Abstract: Compounds linked to a solid support through a divalent linker moiety are disclosed. In particular, compounds such as 1-hydroxybenzotriazole-6-carboxylic acid are directly linked to the support under mild conditions (i.e., in aqueous or organic solvents at neutral pH and at room temperature). The polymer bound 1-hydroxybenzotriazole-6-carboxylic acid can be used for the derivatization of amines as well as for single step amino group modification of proteins, peptides, and amines via acylation or sulfonylation reactions. A flow through device and method for the single step amino group modifications of proteins, peptides, and amines is disclosed. Also disclosed is a flow through device for the detection of amines in a sample. Additionally, a device and method for the detection of amines in a sample using 1-hydroxybenzotriazole-6-carboxylic acid are disclosed. In a preferred embodiment, the device is used to detect the presence of amines in a spoiled meat product.
    Type: Application
    Filed: January 20, 2012
    Publication date: July 19, 2012
    Applicant: The University of Maryland, Baltimore County
    Inventor: Aristotle G. KALIVRETENOS
  • Publication number: 20120183986
    Abstract: Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.
    Type: Application
    Filed: January 25, 2012
    Publication date: July 19, 2012
    Inventors: Zhenjun Diwu, Jinfang Liao
  • Publication number: 20120172582
    Abstract: The present invention relates to methods and reagents for detecting analytes, e.g. nucleic acids. The new methods and reagents allow a simple and sensitive detection even in complex biological samples.
    Type: Application
    Filed: March 2, 2012
    Publication date: July 5, 2012
    Applicant: BASECLICK GMBH
    Inventors: Thomas CARELL, Anja SCHWÖGLER, Glenn A. BURLEY, Johannes GIERLICH, Mohammad Reza MOFID
  • Publication number: 20120138789
    Abstract: Phosphonium-based room temperature ionic liquids (“RTILs”) were prepared. They were used as matrices for Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry and also for preparing samples of dyes for analysis.
    Type: Application
    Filed: December 2, 2010
    Publication date: June 7, 2012
    Applicant: Los Alamos National Security, LLC
    Inventors: Rico E. Del Sesto, Andrew T. Koppisch, Katherine S. Lovejoy, Geraldine M. Purdy
  • Publication number: 20120121536
    Abstract: Glucose deprivation is an attractive strategy in cancer research and treatment. Cancer cells upregulate glucose uptake and metabolism for maintaining accelerated growth and proliferation rates. Specifically blocking these processes is likely to provide new insights to the role of glucose transport and metabolism in tumorigenesis, as well as in apoptosis. As solid tumors outgrow the surrounding vasculature, they encounter microenvironments with a limited supply of nutrients leading to a glucose deprived environment in some regions of the tumor. Cancer cells living in the glucose deprived environment undergo changes to prevent glucose deprivation-induced apoptosis. Knowing how cancer cells evade apoptosis induction is also likely to yield valuable information and knowledge of how to overcome the resistance to apoptosis induction in cancer cells.
    Type: Application
    Filed: March 24, 2011
    Publication date: May 17, 2012
    Applicant: OHIO UNIVERSITY
    Inventors: Xiaozhuo Chen, Stephen Bergmeier
  • Patent number: 8143069
    Abstract: A compound represented by the following general formula (II): wherein R21 represents hydrogen atom or one to four monovalent substituents substituting on the benzene ring, and when R21 represents two or more substituents, the substituents may be the same or different; R22, R24, R25, and R27 independently represent hydrogen atom, or a monovalent substituent; R23 represents —OH or —N(R28)(R29) (wherein R28 and R29 independently represent an alkyl group which may be substituted); R26 represents —N(R30)(R31) (wherein R30 and R31 independently represent an alkyl group which may be substituted); n represents an integer of 1 to 3; and Y represents —S— or —O—, which is useful for selective measurement of hypochlorite ion.
    Type: Grant
    Filed: March 1, 2007
    Date of Patent: March 27, 2012
    Assignee: The University of Tokyo
    Inventors: Tetsuo Nagano, Yasuteru Urano, Suguru Kenmoku
  • Patent number: 8129193
    Abstract: The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
    Type: Grant
    Filed: July 28, 2010
    Date of Patent: March 6, 2012
    Assignee: AnaSpec Incorporated
    Inventors: Jianjun He, Anita Hong, Qingzheng Wang, Raman Khosrow Afshar, Ming Zhu, Vera Rakhmanova
  • Publication number: 20120020893
    Abstract: The invention is directed to a probe-type imaging device useful to visualize interior surfaces, e.g., the lumen of blood vessels. Specifically, the probe-type device is particularly useful for direct tissue imaging in the presence or absence of molecular imaging agents.
    Type: Application
    Filed: October 4, 2011
    Publication date: January 26, 2012
    Inventors: David R. Elmaleh, Rick A. Rogers, Hjalmar Brismar
  • Publication number: 20120009683
    Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
    Type: Application
    Filed: July 29, 2011
    Publication date: January 12, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Richard GEE, Vladimir MARTIN
  • Publication number: 20120004183
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
    Type: Application
    Filed: June 23, 2011
    Publication date: January 5, 2012
    Applicant: The Scripps Research Institute
    Inventors: Alexander Deiters, T. Ashton Cropp, Jason W. Chin, J. Christopher Anderson, Peter G. Schultz
  • Patent number: 8084627
    Abstract: Hydroxymethyl fluorescein derivatives are provided. The derivatives can be easily conjugated to other molecules, and are more permeable than other fluorescein derivatives. Methods of making the derivatives are also provided.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: December 27, 2011
    Assignee: University of Pittsburgh-of the Commonwealth System of Higher Education
    Inventors: Kazunori Koide, Amanda L. Garner, Fengling Song
  • Publication number: 20110287552
    Abstract: A compound represented by the general formula (I) [R1 and R2 are amino groups that substitute at adjacent positions on the benzene ring; R3 and R4 are halogen atoms; R5 and R6 represent hydrogen atom, an acyl group or an acyloxy(C1-6 alkyl) group; R7 and R8 represent —(CH2)p—N(R9)(R10) (p is 1 to 4, and R9 and R10 represent —(CH2)n—COOH (n is 1 to 4))], which is useful for measuring a reactive nitrogen species existing in cells such as nitrogen monoxide or peroxynitrite at high sensitivity over a long period of time.
    Type: Application
    Filed: December 4, 2009
    Publication date: November 24, 2011
    Applicant: THE UNIVERSITY OF TOKYO
    Inventors: Tetsuo Nagano, Yasuteru Urano, Saki Izumi
  • Publication number: 20110251404
    Abstract: A compound represented by the formula (I) (R1 represents an aryl group such as hydroxyphenyl group, R2 represents 2-carboxyphenyl group etc., and R3 and R4 represent —(CH2)p—N(R5)(R6) (R5 and R6 represent —(CH2)n—COOH), a salt thereof, or an ester thereof, which is useful as a reagent for highly sensitive measurement of reactive oxygen such as hydroxy radical present at a trace amount in living bodies or tissues by a bioimaging technique over a long period of time.
    Type: Application
    Filed: May 15, 2009
    Publication date: October 13, 2011
    Applicant: THE UNIVERSITY OF TOKYO
    Inventors: Tetsuo Nagano, Yasuteru Urano, Saki Izumi
  • Publication number: 20110251236
    Abstract: Therapeutic agents for treatment of Galactosemia and disorders thereof, and other enzyme related disorders thereof are identified. The compounds inhibit galactokinase (GALK) and other kinase activity and are identified by a high throughput screening assay.
    Type: Application
    Filed: August 14, 2008
    Publication date: October 13, 2011
    Applicant: University of Miami
    Inventors: Kent Lai, Klass Jan Wierenga, Manshu Tang
  • Publication number: 20110229924
    Abstract: Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.
    Type: Application
    Filed: March 2, 2011
    Publication date: September 22, 2011
    Inventors: Zhenjun Diwu, Jianjun He, Jinfang Liao
  • Publication number: 20110184147
    Abstract: The present invention provides a substrate of TGase represented by (fluorescent group)-(linker)-(a portion containing a Gln residue capable of recognition by transglutaminase (TGase))-R, wherein the fluorescent group is fluorescein isothiocyanate (FITC), Texas Red (TE) or dansyl (Dns) or a group derived therefrom; the linker is a group represented by —NH—(CH2)n—CO— (n is an integer of 1 to 6); the portion containing a Gln residue capable of recognition by TGase is a group derived from a peptide selected from among QG and the like; and R is a hydroxyl group, or biotin, nucleic acid, azide, alkyne, maleimide or cyclopentadiene, or a group derived therefrom.
    Type: Application
    Filed: July 3, 2009
    Publication date: July 28, 2011
    Applicant: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
    Inventors: Noriho Kamiya, Masahiro Goto, Hiroki Abe
  • Patent number: 7985865
    Abstract: A compound having the formula wherein R1, Z, and the carbonyl can be comprised by a common ring, wherein R1 comprises a chromophore that absorbs light from the visible wavelength range, and wherein n is an integer of at least 12. A solid phase change ink composition is also disclosed containing a colorant comprising a chromophore that absorbs light from the visible wavelength range and has the formula wherein R1, Z, and the carbonyl can be comprised by a common ring, and wherein n is an integer of at least 12. Also disclosed is a method of forming a colorant wherein a first compound having the formula is reacted with a second compound having the formula Z(CH2)nCH3 wherein n is an integer of at least 12 to form a third compound having the formula wherein the third compound comprises a chromophore that absorbs light from the visible wavelength range.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: July 26, 2011
    Assignee: Xerox Corporation
    Inventors: Jeffrey H Banning, Michael B. Meinhardt, Donald R Titterington, Clifford R King
  • Patent number: 7982056
    Abstract: The present invention is directed to an improved process for producing substantially pure fluorescein, as well as to substantially pure fluorescein compositions prepared by the process. The invention is particularly directed to the provision of pharmaceutical compositions for use in angiography. The substantially pure fluorescein produced by the process of the present invention is low in color, low in sodium chloride content, and substantially free of pyridine.
    Type: Grant
    Filed: December 4, 2007
    Date of Patent: July 19, 2011
    Assignee: Alcon Research, Ltd.
    Inventors: Gregory Bydlinski, Robert Gregg Harris, Britt S. Scott
  • Publication number: 20110159603
    Abstract: A compound represented by the following general formula (IA) or (IB) (R1 represents an electron withdrawing substituent, R2 and R3 represent a hydrogen atom or a halogen atom; R4 and R3 represent a hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, and R6 represents a hydrogen atom or an alkyl group) or a salt thereof, and a reagent for measuring hydrogen peroxide comprising the compound or a salt thereof.
    Type: Application
    Filed: March 4, 2009
    Publication date: June 30, 2011
    Applicant: THE UNIVERSITY OF TOKYO
    Inventors: Tetsuo Nagano, Yasuteru Urano, Masahiro Abo
  • Publication number: 20110160297
    Abstract: The invention provides fluorescent schweinfurthin analogs of formula(I) which are useful as probes and for the treatment of cancer and other diseases.
    Type: Application
    Filed: June 25, 2009
    Publication date: June 30, 2011
    Applicant: University of Iowa Research Foundation
    Inventors: David F. Wiemer, Jeffrey D. Neighbors, Raymond J. Hohl, Craig Kuder