Rhodamines Patents (Class 549/227)
  • Patent number: 9315672
    Abstract: Provided are a colorant represented by the following Formula (I) which is excellent in a color intensity and a lightfastness, a microcapsule pigment including the colorant, a developer and a chromic temperature controller and an ink composition for writing instruments including the microcapsule pigment: wherein X and Y represent a hydrogen atom, an alkyl group having 1 to 4 carbon atoms or a halogen atom; and X and Y may be identical or different from each other, provided that a case in which both of X and Y are a hydrogen atom is excluded.
    Type: Grant
    Filed: October 5, 2012
    Date of Patent: April 19, 2016
    Assignee: MITSUBISHI PENCIL COMPANY, LIMITED
    Inventor: Shuji Ichikawa
  • Patent number: 9249231
    Abstract: There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: February 2, 2016
    Assignee: Cell Signaling Technology, Inc.
    Inventors: Michael Comb, Ailan Guo, John Edward Rush, II, Jun-Ming Cai, Jing Li, Jing Zhou
  • Publication number: 20140299015
    Abstract: Provided are a colorant represented by the following Formula (I) which is excellent in a color intensity and a lightfastness, a microcapsule pigment prepared by using the above colorant, and an ink composition for writing instruments: [in Formula (I) described above, X and Y represent a hydrogen atom, an alkyl group having 1 to 4 carbon atoms or a halogen atom; and X and Y may be identical or different from each other, provided that a case in which both of X and Y are a hydrogen atom is excluded]. The above microcapsule pigment is characterized by comprising at least the colorant comprising the leuco dye represented by Formula (I) described above, a developer and a chromic temperature controller, and the ink composition for writing instruments is characterized by comprising the above microcapsule pigment.
    Type: Application
    Filed: October 5, 2012
    Publication date: October 9, 2014
    Applicant: MITSUBISHI PENCIL COMPANY, LIMITED
    Inventor: Shuji Ichikawa
  • Patent number: 8802082
    Abstract: Compositions and methods for reducing or preventing graft-versus-host disease associated with allogeneic stem cell transplantation are described. A method includes harvesting hematopoietic cells from a patient with an immunologic disorder and harvesting hematopoietic cells from a donor. The harvested hemaptopoietic cells from the patient and the donor are mixed ex vivo for a period of time sufficient to activate lymphocytes within the hematopoietic cells harvested from the donor such that an immune reaction occurs and an activated portion and an unactivated portion is produced. A rhodamine B derivative is then added to the mixture of cells and the mixture is irradiated at a suitable wavelength and intensity for the selective destruction and/or inactivation of the activated portion without substantially affecting the unactivated portion or causing systemic toxicity in said patient. The irradiated cells are then infused into the patient.
    Type: Grant
    Filed: March 29, 2013
    Date of Patent: August 12, 2014
    Assignees: Universite de Montreal, Hopital Maisonneuve-Rosemont
    Inventors: Denis-Claude Roy, Martin Guimond, Nestor Molfino, Luc Villeneuve
  • Patent number: 8722574
    Abstract: There are described thermal imaging members and thermal imaging methods utilizing unsymmetrical rhodamine compounds. The rhodamine color-forming compounds exhibit a first color when in a crystalline form and a second color, different from the first color, when in an amorphous form.
    Type: Grant
    Filed: April 27, 2012
    Date of Patent: May 13, 2014
    Assignee: Zink Imaging, Inc.
    Inventors: Michael P Filosa, Stephen J. Telfer, John L Marshall, Richard M Allen, John M Hardin
  • Publication number: 20140120564
    Abstract: This invention provides devices, compositions and methods for determining the concentration of one or more metabolites or analytes in a biological sample, including cells, tissues, organs, organisms, and biological fluids. In particular, this invention provides materials, apparatuses, and methods for several non-invasive techniques for the determination of in vivo blood glucose concentration levels based upon the in vivo measurement of one or more biologically active molecules found in skin.
    Type: Application
    Filed: August 12, 2013
    Publication date: May 1, 2014
    Applicant: Cercacor Laboratories, Inc.
    Inventors: Jerome J. Workman, Christopher R. Lambert, Robert L. Coleman
  • Publication number: 20140120158
    Abstract: The invention provides a conjugate comprising (i) a selectivity agent which binds specifically to one or more neurotransmitter transporters selected from the group consisting of a dopamine transporter (DAT), serotonin transporter (SERT) or a norepinephrine transporter (NET) and (ii) a nucleic acid capable of specifically binding to a target molecule which is expressed in the same cell as the neurotransmitter transporter wherein said target molecule is ?-synuclein or the mRNA encoding ?-synuclein. The conjugates of the present invention are useful for the delivery of the nucleic acid to a cell of interest and thus, for the treatment of diseases which require a down-regulation of the protein encoded by the target nucleic acid as well as for the delivery of imaging agents to the cells for diagnostic purposes.
    Type: Application
    Filed: October 28, 2013
    Publication date: May 1, 2014
    Inventors: Andrés Pablo MONTEFELTRO, Gabriel Alvarado Urbina, Analia Bortolozzi Biassoni, Francesc Artigas Pérez, Miquel Vila Bover
  • Publication number: 20140045173
    Abstract: The invention relates to the field of hydrophobic photoaffinity marking by element of probes that are multifunctional lipid compounds. The compounds include at least one chromophore group and two lipid chains, one of which includes a photoreactive group. The compounds can be used for the study and identification of hydrophobic regions or domains within proteins, particularly within viruses.
    Type: Application
    Filed: March 23, 2012
    Publication date: February 13, 2014
    Applicant: UNIVERSITE DE STRASBOURG
    Inventors: Eve-Isabelle Pecheur, Line Bourel-Bonnet, Benoit Hilbold
  • Publication number: 20130337536
    Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.
    Type: Application
    Filed: August 21, 2013
    Publication date: December 19, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Evan Antoulinakis, Kyle Gee, Aleksey Rukavishnikov
  • Patent number: 8518915
    Abstract: The invention relates to pharmacology and medicine, in particular to a class of mitochondrially-addressed compounds which can be used in the pharmaceutical compositions of medicinal agents (preparations) for preventing and treating cardiovascular diseases and diseases and pathological conditions caused by disturbed blood circulation or oxygen supply to tissues and organs.
    Type: Grant
    Filed: June 29, 2007
    Date of Patent: August 27, 2013
    Assignee: Mitotech SA
    Inventors: Maxim Vladimirovich Skulachev, Vladimir Petrovich Skulachev
  • Patent number: 8435800
    Abstract: The present invention relates in general to labeling reagents useful for labeling biomolecules. In particular, the invention provides activated labeling reagents having the formula L-Ph, wherein L is an activated labeling molecule and Ph is a phenol. The invention further provides methods of preparing the labeling reagents, methods of using the labeling reagents for synthesizing a labeled biomolecule, kits that include reagents for labeling a biomolecule and kits containing labeled biomolecules.
    Type: Grant
    Filed: November 22, 2005
    Date of Patent: May 7, 2013
    Assignee: BioSight Ltd.
    Inventor: Stela Gengrinovitch
  • Patent number: 8436170
    Abstract: The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: May 7, 2013
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung
  • Publication number: 20130102021
    Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
    Type: Application
    Filed: September 13, 2012
    Publication date: April 25, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Daniel BEACHAM, Jeffrey Dzubay, Kyle Gee, Vladimir Martin, Aleksey Rukavishnikov
  • Patent number: 8420402
    Abstract: The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
    Type: Grant
    Filed: January 27, 2012
    Date of Patent: April 16, 2013
    Assignee: AnaSpec Incorporated
    Inventors: Jianjun He, Anita Hong, Qingheng Wang, Raman Khosrow Afshar, Ming Zhu, Vera Rakhmanova
  • Publication number: 20130089853
    Abstract: Provided are various compounds comprising the formula Also provided are fluorescent dyes comprising the above compound. Additionally, a fluorescence energy transfer system is provided that comprises the above-described fluorescent dye and a second dye, wherein the second dye is capable of energy transfer with the fluorescent dye. Further provided is a kit for labeling a target molecule, where the kit comprises the above-described fluorescent dye with additional reagents useful for labeling the target molecule. Additionally provided is a target molecule labeled with the above-described fluorescent dye. A method of labeling a target molecule is also provided. The method comprises contacting reactive group Z of the above-described fluorescent dye with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between reactive group Z and the target molecule. Also, another method of labeling a target molecule is provided.
    Type: Application
    Filed: October 11, 2011
    Publication date: April 11, 2013
    Applicant: ENZO LIFE SCIENCES, INC., C/O ENZO BIOCHEM, INC.
    Inventors: ZAIGUO LI, PRAVEEN PANDE
  • Patent number: 8409564
    Abstract: The present invention relates to the use of the photoactivable derivatives for the photodynamic treatment for the selective destruction and/or inactivation of immunologically reactive cells without affecting the normal cells and without causing systemic toxicity for the patient, wherein appropriate intracellular levels of said derivatives are achieved and irradiation of a suitable wavelength and intensity is applied.
    Type: Grant
    Filed: October 21, 2004
    Date of Patent: April 2, 2013
    Assignees: Universite de Montreal, Hopital Maisonneuve-Rosemont
    Inventors: Denis-Claude Roy, Martin Guimond, Nestor Molfino, Luc Villeneuve
  • Publication number: 20130059726
    Abstract: There are described thermal imaging members and thermal imaging methods utilizing unsymmetrical rhodamine compounds. The rhodamine color-forming compounds exhibit a first color when in a crystalline form and a second color, different from the first color, when in an amorphous form.
    Type: Application
    Filed: April 27, 2012
    Publication date: March 7, 2013
    Inventors: Michael P. Filosa, Stephen J. Telfer, John L. Marshall, Richard M. Allen, John M. Hardin
  • Publication number: 20130052746
    Abstract: Organic dyes that are covalently bonded multimers or higher order symmetrical structures where one or more dye units may optionally contain deuterium substitutions are disclosed. The disclosed dyes are particularly useful for optical coatings, new optical nanomaterials, high density storage devices. The structural and optical characteristics of the disclosed dyes provide avenues for increased information-content through surface-enhanced Raman resonance spectroscopy.
    Type: Application
    Filed: March 15, 2011
    Publication date: February 28, 2013
    Applicant: Purdue Research Foundation
    Inventors: Meiguo Xin, Vincent Jo Davisson
  • Patent number: 8383672
    Abstract: Methods for treating a bacterial infection in a tissue sample are described. Tissue samples that are harvested from a patient may be infected with bacteria. A rhodamine compound is mixed with the infected tissue sample to form a mixture. The mixture is then exposed to radiant energy to inhibit or kill the bacteria. The exposed mixture is then transplanted into the patient.
    Type: Grant
    Filed: June 9, 2011
    Date of Patent: February 26, 2013
    Assignee: Kiadis Pharma Canada Inc.
    Inventors: Abdelkrim Habi, Denis Gravel, Luc Villeneuve, Hongsheng Su, Marc Vaillancourt
  • Publication number: 20120172583
    Abstract: Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
    Type: Application
    Filed: September 14, 2010
    Publication date: July 5, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda G. Lee, Sandra L. Spurgeon, Barnett B. Rosenblum
  • Publication number: 20120138789
    Abstract: Phosphonium-based room temperature ionic liquids (“RTILs”) were prepared. They were used as matrices for Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry and also for preparing samples of dyes for analysis.
    Type: Application
    Filed: December 2, 2010
    Publication date: June 7, 2012
    Applicant: Los Alamos National Security, LLC
    Inventors: Rico E. Del Sesto, Andrew T. Koppisch, Katherine S. Lovejoy, Geraldine M. Purdy
  • Publication number: 20120135459
    Abstract: The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.
    Type: Application
    Filed: June 26, 2009
    Publication date: May 31, 2012
    Applicant: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
    Inventors: Stefan W. Hell, Vladimir N. Belov, Gyuzel Mitronova, Mariano Bossi, Gael Moneron, Christian A. Wurm, Stefan Jakobs, Christian Eggeling, Jakob Bierwagen, Lars Meyer
  • Patent number: 8143069
    Abstract: A compound represented by the following general formula (II): wherein R21 represents hydrogen atom or one to four monovalent substituents substituting on the benzene ring, and when R21 represents two or more substituents, the substituents may be the same or different; R22, R24, R25, and R27 independently represent hydrogen atom, or a monovalent substituent; R23 represents —OH or —N(R28)(R29) (wherein R28 and R29 independently represent an alkyl group which may be substituted); R26 represents —N(R30)(R31) (wherein R30 and R31 independently represent an alkyl group which may be substituted); n represents an integer of 1 to 3; and Y represents —S— or —O—, which is useful for selective measurement of hypochlorite ion.
    Type: Grant
    Filed: March 1, 2007
    Date of Patent: March 27, 2012
    Assignee: The University of Tokyo
    Inventors: Tetsuo Nagano, Yasuteru Urano, Suguru Kenmoku
  • Patent number: 8129193
    Abstract: The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.
    Type: Grant
    Filed: July 28, 2010
    Date of Patent: March 6, 2012
    Assignee: AnaSpec Incorporated
    Inventors: Jianjun He, Anita Hong, Qingzheng Wang, Raman Khosrow Afshar, Ming Zhu, Vera Rakhmanova
  • Patent number: 8101655
    Abstract: A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O? or S?; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through, the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.
    Type: Grant
    Filed: January 27, 2010
    Date of Patent: January 24, 2012
    Inventors: Gerald Arthur Smith, Darren William Watson, Howard Paul Voorheis
  • Publication number: 20120003301
    Abstract: Provided are methods of applying a tissue marking to a tissue and rendering said tissue marking colorless when desired, comprising implanting into the tissue an amount of the tissue marking in sufficient quantity to form a detectable marking, said tissue marking comprising at least one colored compound comprising a thermally activatable fragmentation group and at least one infrared absorbing compound, wherein the at least one colored compound is capable of being rendered colorless by unimolecular fragmentation of the thermally activatable fragmentation group when the tissue marking is non-imagewise exposed by a source of infrared radiation and applying sufficient infrared radiation to a sufficient amount of the tissue marking to render the tissue marking colorless when desired.
    Type: Application
    Filed: September 15, 2011
    Publication date: January 5, 2012
    Applicant: PERFORMANCE INDICATOR LLC
    Inventors: Satish Agrawal, Roger Boggs
  • Patent number: 7985865
    Abstract: A compound having the formula wherein R1, Z, and the carbonyl can be comprised by a common ring, wherein R1 comprises a chromophore that absorbs light from the visible wavelength range, and wherein n is an integer of at least 12. A solid phase change ink composition is also disclosed containing a colorant comprising a chromophore that absorbs light from the visible wavelength range and has the formula wherein R1, Z, and the carbonyl can be comprised by a common ring, and wherein n is an integer of at least 12. Also disclosed is a method of forming a colorant wherein a first compound having the formula is reacted with a second compound having the formula Z(CH2)nCH3 wherein n is an integer of at least 12 to form a third compound having the formula wherein the third compound comprises a chromophore that absorbs light from the visible wavelength range.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: July 26, 2011
    Assignee: Xerox Corporation
    Inventors: Jeffrey H Banning, Michael B. Meinhardt, Donald R Titterington, Clifford R King
  • Publication number: 20110111446
    Abstract: It is an object of the present invention to provide: a novel thiol-detecting reagent, which can be used in vivo and which solves the problem regarding the generation of background fluorescence due to hydrolysis; and a method for detecting thiol using the aforementioned reagent. The present invention provides a compound represented by the following formula (1): wherein each R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom.
    Type: Application
    Filed: January 30, 2009
    Publication date: May 12, 2011
    Applicant: RIKEN
    Inventors: Hiroshi Abe, Yoshihiro Ito, Aya Shibata, Mika Ito
  • Publication number: 20110105754
    Abstract: There are described novel rhodamine color-forming compounds. The rhodamine color-forming compounds exhibit a first color when in a crystalline form and a second color, different from the first color, when in an amorphous form.
    Type: Application
    Filed: November 3, 2010
    Publication date: May 5, 2011
    Inventors: Michael P. Filosa, John L. Marshall, Richard M. Allen
  • Publication number: 20110054165
    Abstract: The invention provides a novel class of xanthene dyes, some of which are functionalized to allow their coupling to conjugation partners of interest, e.g. biomolecules, drugs, toxins and the like. Also provided are conjugates of the dyes, methods of preparing and using the dyes and their conjugates and kits including the dyes and their conjugates.
    Type: Application
    Filed: November 8, 2010
    Publication date: March 3, 2011
    Applicant: BIOSEARCH TECHNOLOGIES, INC.
    Inventors: MARK REDDINGTON, MATT LYTTLE
  • Publication number: 20100280252
    Abstract: The present invention provides bifunctional polymers, methods of preparing the same, and intermediates thereto. These compounds are useful in a variety of applications including the PEGylation of biologically active molecules. The invention also provides methods of using said compounds and compositions thereof.
    Type: Application
    Filed: February 17, 2010
    Publication date: November 4, 2010
    Applicant: Intezyne Technologies
    Inventors: Kurt Breitenkamp, Kevin N. Sill, Habib Skaff
  • Publication number: 20100197030
    Abstract: The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
    Type: Application
    Filed: February 3, 2010
    Publication date: August 5, 2010
    Applicant: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung
  • Patent number: 7745645
    Abstract: Compounds having the general of formula I and/or formula II wherein R1 and R2 are the same or different and are independently selected from the group consisting of —H, —C1-C18-alkyl or -?-sulfoalkyl; X and Y are the same or different and are independently selected from the group consisting of —O?, —OH, —SH, —NH—NH2, —F, —Cl, —Br, —I, —O-Su (succinimidyl/sulfosuccinimidyl), —O-STP (4-sulfo-2,3,5,6-tetrafluorophenyl), —O-TFP (2,3,5,6-tetrafluorophenyl), —O-benzotriazole, -benzotriazole, —NR—CO—CH2—I, —NR2, —NR-biomolecule, —NR-L-COO?, —NR-L-COOH, —NR-L-COO-Su, —NR-L-COO-STP, —NR-L-COO-TFP, —NR-L-CONR2, —NR-L-CO-biomolecule, —NR-L-CO—NH—NH2, —NR-L-OH, —NR-L-O-phosphoramidite, —NR-L-CHO, —NR-L-maleimid, or —NR-L-NH—CO—CH2—I; where R is equal to R1 and R2 and L is selected from the group consisting of a divalent linear (—(CH2)o—, o=1 to 15), crossed, or cyclic alkane group that can be substituted by at least one atom selected from the group consisting of oxygen, substituted ni
    Type: Grant
    Filed: January 22, 2007
    Date of Patent: June 29, 2010
    Assignee: Pierce Biotechnology, Inc.
    Inventors: Wilhelm G. Frank, Matthias S. Wenzel, Peter T. Czerney, Surbhi Desai, Greg Hermanson
  • Patent number: 7704667
    Abstract: There are described novel rhodamine dye compounds and imaging members and imaging methods, including thermal imaging members and imaging methods, utilizing the compounds. The dye compounds exhibit a first color when in the crystalline form and a second color, different from the first color, when in the liquid, amorphous form.
    Type: Grant
    Filed: January 30, 2008
    Date of Patent: April 27, 2010
    Assignee: Zink Imaging, Inc.
    Inventors: Kap-Soo Cheon, Stephen J. Telfer, Michael P. Filosa, John L. Marshall, Fariza B. Hasan, David A. Skyler, John M. Hardin
  • Patent number: 7674918
    Abstract: A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, alkaryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O? or S?; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through. the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.
    Type: Grant
    Filed: April 6, 2005
    Date of Patent: March 9, 2010
    Assignee: Cambridge Enterprise Limited
    Inventors: Gerald Arthur Smith, Darren William Watson, Howard Paul Voorheis
  • Patent number: 7667051
    Abstract: The invention relates to a method of preparing urethane-protected N-carboxyanhydrides of alpha amino acids. The inventive method enables the synthesis of urethane-protected N-carboxyanhydrides of alpha amino acids in the presence of a catalytic quantity of triethylene diamine without the addition of a tertiary amine-type base.
    Type: Grant
    Filed: August 17, 2004
    Date of Patent: February 23, 2010
    Assignee: ISOCHEM
    Inventors: Hervé Lhermitte, Julien Grima, Antoine Paris
  • Patent number: 7560574
    Abstract: Compounds of formula (I) wherein: one of R1, R2, R3, R4 and (R10)n represents halogen and each remaining R1, R2, R3, R4 and R10 group is independently selected from hydrogen, halogen, amino, acylamino, dialkylamino, cycloalkylamino, azacycloalkyl, alkylcycloalkylamino, aroylamino, diarylamino, arylalkylamino, aralkylamino, alkylaralkylamino, arylaralkylamino, hydroxy, alkoxy, aryloxy, aralkyloxy, mercapto, alkylthio, arylthio, aralkylthio, carboxyl, alkoxycarbonyl, aryloxycarbonyl, aralkoxycarbonyl, carbamoyl, alkylcarbamoyl, dialkylcarbamoyl, cyano, hydroxysulfonyl, amidosulfonyl, dialkylamidosulfonyl, arylalkylamidosulfonyl, formyl, acyl, aroyl, alkyl, alkylene, alkenyl, aryl, aralkyl, vinyl, alkynyl, and corresponding substituted groups; m=0-1; n=1-4, A is nil, O, or NH; R9 is alkylene; -Z is H, amino, dialkylamino, or a trialkylamino salt; X? is an anion; R5, R6, R7 and R8 are independently H or C1-C6 alkyl or R1 in combination with R5 or R6, or R2 in combination with R5 or R6, or R3 in combination wi
    Type: Grant
    Filed: March 27, 2002
    Date of Patent: July 14, 2009
    Assignee: Celmed Biosciences Inc.
    Inventors: Abdelkrim Habi, Denis Gravel, Luc Villeneuve, Hongsheng Su, Marc Vaillancourt
  • Publication number: 20090176869
    Abstract: Compounds of formula I: in which one of R1, R2, R3, R4 and (R10)n represents halogen and each of the remaining R1, R2, R3, R4 and R10 groups is independently selected from hydrogen, halogen, amino, acylamino, dialkylamino, cycloalkylamino, azacycloalkyl, alkylcycloalkylamino, aroylamino, diarylamino, arylalkylamino, aralkylamino, alkylaralkylamino, arylaralkylamino, hydroxyl, alkoxy, aryloxy, aralkyloxy, mercapto, alkylthio, arylthio, aralkylthio, carboxyl, alkoxycarbonyl, aryloxycarbonyl, aralkoxycarbonyl, carbamoyl, alkylcarbamoyl, dialkylcarbamoyl, cyano, hydroxysulfonyl, amidosulfonyl, dialkylamidosulfonyl, arylalkylamido-sulfonyl, formyl, acyl, aroyl, alkyl, alkylene, alkenyl, aryl, aralkyl, vinyl, alkynyl and corresponding substituted groups; m=0-1; n=1-4; A is nil, O or NH; R9 is alkylene, Z is H, amino, dialkylamino or trialkylamino salt; X is an anion; and R5, R6, R7 and R8 are independently selected from H and C1-6 alkyl, or R1 with R5 or R6, or R2 with R5 or R6, or R3 with R7 or R8 represents a
    Type: Application
    Filed: March 13, 2009
    Publication date: July 9, 2009
    Applicant: CELMED BIOSCIENCE INC.
    Inventors: Abdelkrim Habi, Denis Gravel, Luc Villeneuve, Jean-Pierre Forte, Hongsheng Su, Marc Vaillancourt
  • Patent number: 7534902
    Abstract: Latent fluorescent compounds, comprising a fluorescent molecule with one or more blocking groups attached and optionally one or more urea-containing groups are provided. The urea-containing group can be used to further attach one or more molecules of interest, such as proteins, peptides or nucleic acids. The blocking group(s) is released from the latent fluorescent compound by reaction with a trigger, forming the fluorescent molecule which can be detected. Also provided herein are methods of using latent fluorescent compounds to detect triggers.
    Type: Grant
    Filed: November 15, 2004
    Date of Patent: May 19, 2009
    Assignees: Wisconsin Alumni Research Foundation, The Penn State Research Foundation
    Inventors: Ronald T. Raines, Sunil S. Chandran, Timothy E. Glass, Luke D. Lavis
  • Patent number: 7432298
    Abstract: Fluorescent polymeric materials are disclosed comprising a polymer and one or more lipid soluble rhodamine dyes. The materials are especially useful in the preparation of multicolored microparticles, especially multicolored polystyrene microparticle, for use in the multiplexed analysis of a plurality of analytes in a single sample. When excited by a light source, the materials give off a unique emission based on the nature, concentration and ratio of the dyes therein. Methods of preparing and using said materials are also disclosed.
    Type: Grant
    Filed: May 4, 2004
    Date of Patent: October 7, 2008
    Assignee: Applied Biosystems Inc.
    Inventors: Joe Y. L. Lam, Steven M. Menchen, Ruiming Zou, Scott C. Benson
  • Publication number: 20080177086
    Abstract: Compounds having the general of formula I and/or formula II wherein R1 and R2 are the same or different and are independently selected from the group consisting of —H, —C1-C18-alkyl or -?-sulfoalkyl; X and Y are the same or different and are independently selected from the group consisting of —O?, —OH, —SH, —NH—NH2, —F, —Cl, —Br, —I, —O-Su (succinimidyl/sulfosuccinimidyl), —O-STP (4-sulfo-2,3,5,6-tetrafluorophenyl), —O-TFP (2,3,5,6-tetrafluorophenyl), —O-benzotriazole, -benzotriazole, —NR—CO—CH2—I, —NR2, —NR-biomolecule, —NR-L-COO?, —NR-L-COOH, —NR-L-COO-Su, —NR-L-COO-STP, —NR-L-COO-TFP, —NR-L-CONR2, —NR-L-CO-biomolecule, —NR-L-CO—NH—NH2, —NR-L-OH, —NR-L-O-phosphoramidite, —NR-L-CHO, —NR-L-maleimid, or —NR-L-NH—CO—CH2—I; where R is equal to R1 and R2 and L is selected from the group consisting of a divalent linear (—(CH2)o—, o=1 to 15), crossed, or cyclic alkane group that can be substituted by at least one atom selected from the group consisting of oxygen, substituted ni
    Type: Application
    Filed: January 22, 2007
    Publication date: July 24, 2008
    Applicant: PIERCE BIOTECHNOLOGY, INC.
    Inventors: Wilhelm G. Frank, Matthias S. Wenzel, Peter T. Czerney, Surbhi Desai, Greg Hermanson
  • Patent number: 7399639
    Abstract: The present invention is directed, in part, to sensors for detecting metal ions, and methods of making and using the same.
    Type: Grant
    Filed: May 4, 2003
    Date of Patent: July 15, 2008
    Assignee: Massachusetts Institute of Technology
    Inventors: Stephen J. Lippard, Carolyn Crystal Woodroofe
  • Patent number: 7282575
    Abstract: A compound represented by the general formula (I) below: In the formula, A is as defined in the specification and B denotes where n is an integer of 1 to 4, and a process for producing the above compound includes a reaction between an activated ester and a t-butoxycarbonylaminoethylamine or an ?-amino acid derivative.
    Type: Grant
    Filed: September 19, 2001
    Date of Patent: October 16, 2007
    Assignee: Credia Japan Co., Ltd.
    Inventors: Hisafumi Ikeda, Isao Saito, Fumihiko Kitagawa
  • Patent number: 7102005
    Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
    Type: Grant
    Filed: May 2, 2003
    Date of Patent: September 5, 2006
    Assignee: Molecular Probes, Inc.
    Inventors: Brian Agnew, Joseph Beechem, Kyle Gee, Richard Haugland, Jixiang Liu, Vladimir Martin, Wayne Patton, Thomas Steinberg
  • Patent number: 7083667
    Abstract: Disclosed herein is a colorant compound represented by the following general formula (1): wherein R1 and R2 are the same or different from each other and denote a linear alkyl group having 1 to 4 carbon atoms, R3 is an alkyl group having 1 to 6 carbon atoms, and An? is a counter anion, an ink comprising the colorant compound, and an ink tank, a recording unit, a recording apparatus and a recording process comprising or using the ink.
    Type: Grant
    Filed: February 9, 2005
    Date of Patent: August 1, 2006
    Assignee: Canon Kabushiki Kaisha
    Inventors: Yasuaki Murai, Takayuki Toyoda, Takeshi Miyazaki, Yuko Yamagishi
  • Patent number: 7049073
    Abstract: This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.
    Type: Grant
    Filed: October 30, 2002
    Date of Patent: May 23, 2006
    Assignee: The University of Chicago
    Inventors: Boris Chernov, Julia Golova
  • Patent number: 7008759
    Abstract: There are described novel rhodamine dye compounds and imaging members and imaging methods, including thermal imaging members and imaging methods, utilizing the compounds. The dye compounds exhibit a first color when in the crystalline form and a second color, different from the first color, when in the liquid, amorphous form.
    Type: Grant
    Filed: February 27, 2004
    Date of Patent: March 7, 2006
    Assignee: Polaroid Corporation
    Inventors: Kap-Soo Cheon, Michael P. Filosa, John L. Marshall
  • Patent number: 6998493
    Abstract: Compounds of the formula wherein M is either (1) a metal ion having a positive charge of +p wherein p is an integer which is at least 2, said metal ion being capable of forming a compound with at least two chromogen moieties, or (2) a metal-containing moiety capable of forming a compound with at least two chromogen moieties, z is an integer representing the number of chromogen moieties associated with the metal and is at least 2, and R1, R2, R3, R4, R5, R6, R7, a, b, c, d, Y, Q?, A, and CA are as defined herein.
    Type: Grant
    Filed: June 26, 2003
    Date of Patent: February 14, 2006
    Assignee: Xerox Corporation
    Inventors: Jeffery H. Banning, Bo Wu, James M. Duff, Wolfgang G. Wedler, Jule W. Thomas, Jr., Randall R. Bridgeman
  • Patent number: 6967250
    Abstract: Energy transfer dyes, their preparation, and their use as labels in biological systems is disclosed. The dyes are preferably in the form of cassettes which enable their attachment to a variety of biological materials. The dyes and the reagents that can be made from them offer a wide variety of fluorescent labels with large Stokes' shifts enabling their use in a variety of fluorescence applications over a wide range of the visible spectrum.
    Type: Grant
    Filed: August 30, 1999
    Date of Patent: November 22, 2005
    Assignee: Amersham Biosciences Corp
    Inventors: Shiv Kumar, Satyam Nampalli, Mahesh Khot
  • Patent number: 6916925
    Abstract: Dye labeled imidazonaphthyridine, imidazopyridine and imidazoquinoline compounds having immune response modulating activity are disclosed. The compounds arc useful, inter alia, for determining the binding and/or receptor sites of the molecules.
    Type: Grant
    Filed: July 22, 2003
    Date of Patent: July 12, 2005
    Assignee: 3M Innovative Properties Co.
    Inventors: Michael J. Rice, Mark A. Tomai, Ai-Ping Wei