Abstract: Method and apparatus for removing a natural lens from an eye provides for inserting an infusion cannula into an anterior chamber of an eye and establishing fluid communication into a lens capsule from the anterior chamber. A phacoemulsification needle is inserted into the lens capsule for emulsification of a natural lens while introducing irrigation fluid into the anterior chamber and lens capsule through the infusion cannula. Emulsified lens is aspirated through a lumen in the phacoemulsification needle and a sleeve, surrounding the phacoemulsification needle, enables outflow of irrigation fluid in order to maintain intraocular pressure and cool the phacoemulsification needle.

Abstract: An implantable subcutaneous set for mounting onto a skin of a user and contacting a subcutaneous placement site beneath the skin includes a base, a cannula, an insertion device and an externally extending conduit. The base is used to secure the implantable subcutaneous set to the skin of the user. The cannula is coupled to and extends from the base. Also, the cannula has at least one lumen with an end for fluid communication with the subcutaneous placement site. In addition, the cannula has at least one port formed near another end of the lumen opposite the end for fluid communication. The insertion device is operatively coupled to the implantable subcutaneous set and the cannula to facilitate insertion of the cannula to contact the subcutaneous placement site. Also, the insertion device may be withdrawn from the subcutaneous set and the cannula after placing the cannula in contact with the subcutaneous placement site.

Abstract: A single-lumen oocyte collection device, having a cannula which extends axially outward from a proximal end of a hub. The cannula has a side port and an axial port, each linked to a resilient portion of tubing to allow selective occlusion of the lumen thereof. The side port, through the hub, has a fixed relation to a sharpened, bevelled tip of the cannula, allowing even rotation and accurate orientation of the beveled tip of the device to puncture a follicle on an ovary with minimal trauma prior to collecting an oocyte. An echogenic structure is formed at the tip of the cannula.

Abstract: A phacoemulsification instrument that significantly reduces infusion fluid leakage by providing a silicone sleeve with a built in reinforcing member. Also, the sleeve has only a single infusion hole in order to provide better infusion fluid flow without causing turbulence in the eye. In addition, the present invention provides either a valve or a variable aperture aspiration tube in order to regulate the aspiration rate. Also, the present invention includes a single aspiration hole at the tip in order to amplify flow coming to the tip. To assist in cataract removal, the tip may be provided with a variety of different barrier and baffle configuration. A handpiece includes a variable capacity reservoir in the infusion line to account for surges in the aspiration line. The needle includes steps, angles and barriers to focus ultrasonic energy to aid in emulsifying nuclear tissue and to aid in pushing emulsified material in the direction of aspiration flow.

Abstract: A phacoemulsification instrument that significantly reduces infusion fluid leakage by providing a silicone sleeve with a built in reinforcing member. Also, the sleeve has only a single infusion hole in order to provide better infusion fluid flow without causing turbulence in the eye. In addition, the present invention provides either a valve or a variable aperture aspiration tube in order to regulate the aspiration rate. Also, the present invention includes a single aspiration hole at the tip in order to amplify flow coming to the tip. To assist in cataract removal, the tip may be provided with a variety of different barrier and baffle configuration. A handpiece includes a variable capacity reservoir in the infusion line to account for surges in the aspiration line. The needle includes steps, angles and barriers to focus ultrasonic energy to aid in emulsifying nuclear tissue and to aid in pushing emulsified material in the direction of aspiration flow.

Abstract: A phacoemulsification instrument that significantly reduces infusion fluid leakage by providing a silicone sleeve with a built in reinforcing member. Also, the sleeve has only a single infusion hole in order to provide better infusion fluid flow without causing turbulence in the eye. In addition, the present invention provides either a valve or a variable aperture aspiration tube in order to regulate the aspiration rate. Also, the present invention includes a single aspiration hole at the tip in order to amplify flow coming to the tip. To assist in cataract removal, the tip may be provided with a variety of different barrier and baffle configuration. A handpiece includes a variable capacity reservoir in the infusion line to account for surges in the aspiration line. The needle includes steps, angles and barriers to focus ultrasonic energy to aid in emulsifying nuclear tissue and to aid in pushing emulsified material in the direction of aspiration flow.

Abstract: A catheter body includes a main lumen and an auxiliary lumen. The main lumen has an outlet opening located at a distal end of the body and an inlet opening at a proximal end. The auxiliary lumen has an outlet opening located behind and spaced from the distal end a distance of 5-10 cm and an inlet lateral opening located toward the proximal end. An adapter for providing inlet ports communicating with the inlet openings of the main lumen and auxiliary lumen is provided on the catheter body. The adapter includes three members detachably connected to one another. The forward and middle members have threaded portions which engage one another and the middle and rearward members also have threaded portions which engage with one another. Annular seals are provided between the forward and middle members and the middle and rearward members. The adapter can be readily attached to the catheter body and an external device for dosing a liquid medicine can be attached to the adapter.

Abstract: A method and apparatus for the excision and removal of tissue, such as the lens of the eye includes a pencil-like handpiece having a cannula probe extending from the distal end thereof. The probe includes an inner jet tube to direct a high pressure jet of fluid toward a tissue target, and an outer concentric aspiration tube to aspirate and remove fluid and tissue. The jet tube is recessed proximally within the concentric aspiration tube, and the aspiration tube has an end area significantly larger than the end area of the jet tube. These factors cooperate so that the negative pressure exerted by the aspiration tube creates a suction force that offsets and exceeds the force of the fluid jet. The jet tube is connected to a fluid pressure system including a positive displacement pump, a pressure regulator, safety release, control valve, and a pulse former.

Abstract: A needle and stylus system. The system has a hollow tubular needle formed of a rigid material. The needle has an elongated linear intermediate section constituting the majority of the length of the needle and with a curved distal section terminating in an oval opening. A bore is formed through the intermediate and distal sections of an essentially common diameter and the oval opening at the distal end is located in a plane essentially parallel with the axis of the intermediate section and spaced from the exterior surface of the intermediate section. The needle also has a proximal end formed as a radially exterior enlargement with an enlarged cylindrical bore. A tubular stylet has an elongated linear intermediate section and a curved distal section with an oval planar face positionable at the opening at the distal end of the needle when the stylet is inserted within the needle.

Abstract: A septum piercer and sample extractor for removing a sample from a specimen contained in a container closed by a penetrable septum, a needle having a body with a sharp closed point on its first end. The needle extends axially and contains a first channel and a second channel, which extend toward the second end of the needle. A first side port passes through the body into the first channel. A second port passes through the body into the second channel. The first side port is axially farther from the second end than the second side port. The channels are both open near the second end of the needle. The needle is movable axially to pierce the septum. Its first end can be moved through the septum and into the container so that one of the ports is submerged in a fluid sample in the container while the other of the ports remains out of the sample.

Abstract: A catheter has an abutment member formed proximate the distal end thereof. The abutment member abuts a complementary abutment surface formed upon an introducer so as to prevent collapsing of the catheter body during the insertion process. The abutment member preferably comprises an annular abutment shoulder, which reduces the diameter of the lumen of the catheter. An opening formed in the catheter body proximate the tip of the catheter facilitates fluid flow through the lumen of the catheter in the event that the reduced diameter portion of the lumen becomes obstructed. The tip of the catheter preferably comprises a rigid material, so as to assure positive locking action of the abutment member of the catheter with the abutment surface of the introducer during the insertion process.

Abstract: The vascular introduction device is of the lateral arm type. It comprises a tubular body defining an introduction channel enabling, after installation of the distal part of the introduction device in a blood vessel of a patient to be treated, the introduction of a catheter into the vascular system up to where the operation is be performed. The introduction device is provided over part of its length with a longitudinal bulge whose inner wall defines a cavity merged with the introduction channel and communicating directly with the lateral arm. The tubular body is provided with blood inlet orifices arranged at least in the wall defining the cavity. The lateral arm thus enables feeding of a closed external blood circuit comprising a perfusion pump, with a sufficient blood rate in terms of pressure and flow, equal to the rate that would be obtained by a contralateral puncture point. The blood thus sampled off can be reinjected into the patient via the dilatation catheter.

Abstract: A medical coupling device (8) as part of a medical system for withdrawing an ovum from a follicle. The coupling device has an inner chamber (3) that is formed from first (1) and second (2) parts, which are press-fitted together or slidably fitted together by means of male and female engagement parts (6). The inner chamber has a pair of axial openings (4) for receiving a first conduit (9) and a lateral opening (5) for receiving a second conduit (10) therein. In one embodiment, the first conduit includes a single lumen and a side port (11) of which the second conduit is in fluid communication with the single lumen of the first conduit in the inner chamber of the coupling device. In a second embodiment, the first conduit includes multiple lumens (12,13), of which only one of the lumens of the first conduit has a side port that is in fluid communication with the second conduit in the inner chamber of the coupling device.

Abstract: A two-channel sampling needle is designed for withdrawing body fluids, such as blood or tissue fluid, has two essentially parallel cannulas, the first cannula being provided with an insertion needle which may be removed after the sampling needle has been introduced, its sharpened end projecting beyond the end of the first cannula. At the distal end of the sampling needle the second cannula has at least one connecting opening into the first cannula. A mounting part of the sampling needle is provided with fittings for the two cannulas and with a separate opening plugged by a flexible stopper for removal of the insertion needle.

Abstract: A dialytic probe is provided with an insertion needle sharpened at one end, which is surrounded by a flexible plastic cannula, and may be removed after introduction of the probe. The plastic cannula is covered at least partly by a tubular dialyzing membrane which is bonded to the plastic cannula at the distal end and to a handle at the other end, thus forming a fluid-tight seal. The space between the plastic cannula and dialyzing membrane is divided along the length of the probe, such that two passages or lumens of essentially crescent-shaped cross-section are formed, with a flow-connection at the distal end of the plastic cannula, and with a connection to one of separate ingoing and outgoing lines each. The lumen of the plastic cannula may be connected to a drug feeder line. The handle holds the ingoing and outgoing lines and a potential drug feeder line of the probe.

Abstract: A parameter of blood is sensed in vivo with a system which includes a catheter and a probe. The catheter has a lumen extending therethrough, a proximal end, a distal end and a distal opening at the distal end. The probe includes one or more sensing elements at its distal end. In one embodiment, the sensing element or elements are located at least about 4 mm proximal of the distal opening of the lumen. A volume oscillator is provided to control the movement of blood into and out of the lumen. A flush solution is introduced into the lumen so that when the volume oscillator is inactive the sensing element or elements are exposed to pure flush solution, which preferably includes an anti-clotting component. The probe is received within the catheter and affixed thereto. The volume oscillator is activated to move blood into and out of the lumen to expose the sensing element or elements to blood so that they can sense the blood parameter or parameters of interest.

Abstract: A surgical device has a first cannula formed to define a first interior channel and a second cannula positioned substantially within the first interior channel of the first cannula. The second cannula is formed to define a second interior channel. Both the first cannula and the second cannula are attached to a conduit piece that defines a first passageway in fluid communication with the first interior channel of the first cannula and a second passageway in fluid communication with the second interior channel of the second cannula. A third passageway defined by the conduit piece is in fluid communication with the first interior channel of the first cannula. The second passageway is covered by a breachable seal that permits insertion of instruments therethrough while limiting undesired fluid flow.

Abstract: A catheter device useful for treatment of the pleural space. The device includes a catheter tube having an internal end and an external end and is sized for use in the pleural space. The tube has a central bore forming a first lumen with a flared suction engaging portion on the external end and a tapered portion on the internal end for providing access to the pleural space. Also included is a stylette sized for insertion in the first lumen, the stylette having less flexibility than the tube. Forming part of the device is a second lumen axially extending in the catheter tube from the external end to a closed terminal point proximate the internal end of the tube and having a substantially smaller diameter than the first lumen, the second lumen having at least one opening proximate the closed terminal point, the second lumen being fitted with a flexible tube having an injection port at its exterior end and an open end bonded to the internal wall of the second lumen.

Abstract: A device for combined spinal-epidural anesthesia, including an epidural needle having a bent free end portion with an inclined, pointed tip, and a hub fixedly connected to its other end and a tubular guide needle for a spinal needle. The guide needle has two inclined tips and is fixedly attached along at least part of its length to at least a portion of the straight section of the epidural needle. The guide needle is substantially shorter than the epidural needle and extends between a point located behind the bent free end portion of the epidural needle and a point located ahead of the hub thereof.

Abstract: A dual-lumen oocyte collection device is described. The device is unique in that it includes parallel cannulas which extend axially outward from a proximal end of a hub. The device is also unique in that it further includes a finger depression on the hub. This depression located on the hub directly corresponds to an elongated beveled point of a cannula that extends from a distal end of the hub. The unique parallel cannulas and the finger depression allow medical personnel to evenly rotate and accurately orientate the beveled tip of the device to puncture a follicle on an ovary with minimal trauma prior to collecting an oocyte.

Abstract: Surgical procedures on biological tissue are carried out with the aid of a laser with substances being applied to the tissue before or during the treatment and these substances are absorbent in the range of the wavelength of the laser beam. A surgical manipulator can be provided and equipped with suction and irrigation channels and the substances can be directed to one of these channels via a metering unit. The application of substances such as sulfisomidine or sulfacetamide increases the ablation rate and reduces the threshold of the ablation process. At the same time, sensitive structures are protected from the damaging effects of the laser radiation as well as from the stray radiation and fluorescence radiation triggered by the laser radiation during the application of microsurgery to the eye.

Abstract: A novel biopsy needle like surgical instrument to obtain both solid bone marrow biopsy and liquid bone marrow aspirate by a new method of obtaining both specimens in a single procedure are disclosed. The needle consists of two heads at the proximal end, two lumina inside its elongated body and two distal openings. Each head contains portal for entry for respective stylets for biopsy and aspiration. The distal end of the needle contains two openings leading to the lumina inside the needle meant for biopsy and aspiration respectively. The proximal one third of the needle is encased in a specially designed handle with grips for the index finger, the thumb and a cap for the hollow of the operators hand.Upon insertion of the needle in the cortical bone of the iliac crest of the patient the biopsy stylet is withdrawn. Further pushing the needle results in entry of the marrow cavity facilitating entry of a solid core of marrow in the hollow biopsy portion of needle lumen.

Abstract: A cannula adapted for antegrade administration of cardioplegic solutions during surgical procedures on the heart. The cannula includes a flow lumen having a smooth interior surface to reduce hemolysis when blood is passed therethrough during reperfusion. Structures are included to allow the wall of the vessel, generally the aortic root, to be readily pierced so that fluid may pass through the flow lumen of the cannula into the heart. A suture flange is provided to secure the cannula to the heart. A pressure lumen is provided to communicate the pressure within the heart to a location where it can be monitored. A vent line is also provided to allow a surgeon to readily vent fluids out of the heart.

Abstract: A fine-needle aspirator for collecting a biosample from a subject into a closed sterile system. The aspirator is adapted for use with a variety of needles and comprises a motor driven pump capable of continuous suction in a biosample collection system. The biosample collection system may comprise a biosample connector such as elastomeric tubing in communication with the needle and a collection space in a biosample container whereby a biosample collected by the needle may be transferred to the collection space. A flushing substance flushes the biosample through the biosample collection system. Additives may be added to the biosample after collection in order to treat, preserve or analyze the biosample.

Abstract: The present invention discloses a method for producing a multi-lumen needle suitable for administrating spinal anesthesia. The method will provide a needle that will allow the simultaneous administration of spinal and epidural anesthetic from a single site. A preferred needle made by this method can optionally be made with a stock configuration which has means for holding a catheter during the insertion of a spinal needle and is configured so as to provide gripping sites to assist in positioning the needle. The preferred needle of the present invention has a lumen which can serve as a spinal introducer. The introducer lumen sheaths the spinal needle until the spinal needle is in the epidural space. This assures that the spinal needle will not be bent or fractured in use by impingement with bone or hard tissue.

Abstract: A spinal needle for performing medical treatment is inserted into the vertebral pulp, a laser probe is inserted into the spinal needle, and then laser rays are radiated onto the vertebral pulp. An air-escape tube is unitarily provided parallel to the spinal needle or a flow-passage connected with the interior of the spinal needle is provided in the vicinity of the handle portion of the spinal needle.

Abstract: The injection equipment for an endoscope comprises a guide tube (5) to be inserted into the instrument channel of the endoscope, in which two flexible tubes (7, 9) can be slid to supply the differing viscous fluid constituents in a two constituent fabric glue. At the distal end of the flexible tubes (7, 9) a twin tubular needle (15) is fitted, with its needle tubes (11, 13) opening out separately at the distal end. In this way the two constituents only mix inside the fabric. The flexible tubes (7, 9) also have a differing internal diameter like the two needle tubes (11, 13), whereby the more viscous constituents are fed via the larger diameter flexible tube (7), to which the larger diameter needle tube (11) is also connected. Handling is thereby facilitated and there is better compliance with the correct mixture ratio for both constituents.

Abstract: A biopsy device including a two- or multi-lumen biopsy cannula has a biopsy channel of constant cross section over its entire length and at least one application channel. On its proximal end, it is provided with connection facilities for an aspiration device and at least one application device. In order to enable the collection of tissue specimens of an unchanged structure with the most careful handling possible of the tissue and the application of auxiliary substances into the puncture channel without tissue specimens getting into the application cannula and obstructing the same, the cutting edge is formed by an acute-angularly designed end of the biopsy channel wall alone. At least one application channel is formed by a tube eccentrically slipped over the biopsy channel wall. The biopsy channel wall projects out of the tube with its cutting edge and the tube end encloses an obtuse angle with the biopsy channel.

Abstract: This surgical trocar assembly has the needle of a stylet removably disposed inside a first cannula which is inside a second cannula in a concentric array. The needle has a sharp point to pierce a body part to be drained of fluids. A first fitting on the first cannula has a first nipple to engage an aspiration tube when the needle is removed. The fitting has a tenon to engage in a tubular member of a Y-shaped joint structure. The tubular member has a lateral second nipple to engage an end of a wash fluid feed tube. A second fitting on the second cannula engages on the tubular member so that wash water passes through the tubular member between the first and second cannulas. The first cannula has a tapered sharp open end to enter the body part for aspirating fluids therefrom. The second cannula has a tapered end to engage the outside of the first cannula near its tapered open end, and further has lateral openings to discharge wash fluid from the second cannula to the body part.

Abstract: The needle of the present invention is a multi-lumen needle and preferably a dual-lumen epidural-spinal needle. The needle of the present invention is provided with a hub, which allows for attachment of a syringe to one or more of the lumina, or alternatively the attachment of an adapter to which a syringe can be attached. Preferably the lumina have different cross sectional areas. The smaller of the lumina should be of sufficient size to allow a spinal neelde, guide wire, or micro-catheter to be inserted through the lumen. The larger of the two lumina should preferably be of sufficient size to allow an epidural catheter, spinal needle or guide wire to be introduced. The needle of the present invention has applications of regional anesthesia, chronic and post operative pain management, cricothyroid puncture, intracerebroventricular puncture and, access and drainage of concealed fluid collections within the body.

Abstract: A method and apparatus for aspirating secreted fluids from a wound is disclosed which involves the placement of a drain into a wound via the tissue adjacent the wound. After the wound is closed, a secretion aspiration and collection device, which is connected to the drain via a tube, is placed into operation. The device is designed to have a suction effect controlled between a minimum value and a maximum value according to the quantity of secreted fluid to be aspirated. This is achieved by one or more sensors which control a tube pump. When a second sensor is used, the pump will not be shut off by a passing air bubble in the fluid. A sleeve may also be provided to facilitate introduction of the drain into the wound. Additionally, a needle may be detachably connected to the sleeve to aid the insertion of the sleeve.

Abstract: A patient dependent on extracorporeal treatments is subjected to minimal insertion trauma and pain both before and after a fistula operation. A phlebotomy method is disclosed using a veno-occlusive tourniquet or ligation occluding a venous segment at a location between the inlet and return openings of a dual lumen cathter so that a high blood flow rate can be obtained for an extracorporeal treatment system, thereby lessening or delaying the need for a fistula operation. Registration or alignment of the veno-occlusive tourniquet or ligation is ensured by an attachemnt to the catheter. Preferably the tourniquet or ligation includes an elastic band threaded to the attachment. If a fistula operation becomes necessary or has been performed, insertion trauma and pain is minimized by using a dual-lumen needle having a tapered distal end portion terminating in a beveled cutting edge.

Abstract: Methods for preventing the introduction of air into the vascular system of a patient during intravenous or intraarterial procedures, as well as for preventing the reflex of fluids into the body of a patient. Also, novel fluid directing means and catheters which include integal or attached fluid flow control means for use in these methods.

Abstract: A needle aspiration biopsy device in the form of an external guide catheter having a distal end closed with a rapidly opening and closing valve. A syringe is disposed proximally of the guide catheter and has connected thereto a needle catheter which is telescopically received within the guide catheter. The needle catheter is made of an elongated tube mounting a needle at its distal end. A proximal seal seals the proximal end of the guide catheter and sealingly receives the needle catheter. The needle catheter extends to the distal end of the guide catheter and has a withdrawn position wherein the needle end thereof lies proximally of the valve in the distal end of the guide catheter and an extended position wherein the needle end thereof projects through and distally outwardly of the valve. A source of fluid is connected to the bore within the guide catheter at a location between the proximal seal and the distal valve.

Abstract: A dual-lumen fistula needle for use in hemodialysis treatment. The needle has a gently tapered neck and is divided into arterial and venous lumens by a center septum which is bonded in place. The septum is formed so that the lumens have substantially equal cross sections substantially throughout the length of the needle to promote equal flow rates in the lumens. The diameter of the needle at the tip is similar to that of single-lumen needles in order to reduce discomfort on insertion.

Abstract: An aortic root cannula for use in open heart surgery which combines a luer line for connection with an extracorporeal circuit outside the chest cavity with a left heart vent line and a pressure monitoring line all merging into a base unit having a single cannula for heart wall penetration. A composite base unit is formed of two parts integrated to provide the merging passages to perform the multiple functions.

Abstract: The extension set has two lumen for conveying an intravenous liquid and a drug through a common connector to a catheter for direct infusion of the drug to a patient. The lumen are connected in common to the connector or may be separated by a partition wall. In other embodiments, the drug carrying lumen can be disposed within the intravenous tube and may be of micro-bore size.

Abstract: A transcutaneous access device for permanent placement in blood vessels includes a unitarily constructed, substantially U-shaped tubular member that includes a first leg having means for connecting the transcutaneous access device to external equipment, a mid-portion having at least one aperture, and a second leg terminating in a needle barb. The angular orientation of the device is such that the planes that include the first leg and the second leg that are orthogonal to the mid-portion are substantially parallel, and the second leg is at an acute angle with respect to the plane that includes the mid-portion and said first leg.

Abstract: A perfusion catheter, designed as a double catheter which includes a splint catheter (1) and a mounted second catheter tube (3), which is shorter than the splint catheter (1) and which branches off from splint catheter (1). The splint catheter includes an attached shunt tube (2) and at least one lateral opening (4). A solid rod (7) is inserted into the splint catheter (1) from its back end so as to temporarily close the lateral opening(s) (4) and the shunt (2). The catheter is particularly useful for the isolated perfusion of the liver.

Abstract: A cardioplegia/air aspiration cannula assembly for delivering cardioplegic fluid to and for aspirating air from the aorta during surgery. The assembly includes a hub having a single needle portion. The needle portion has an inner cannula which extends through the hub and defines an inner lumen through which the cardioplegic fluid is injected. An outer cannula is coaxially positioned over the inner cannula and is diametrally spaced therefrom to define an outer lumen. A plurality of holes are formed in the sides of the outer cannula through which air can be aspirated. The outer lumen defined by the space between the inner and outer cannulae is in fluid communication with a passageway passing through a side branch of the hub which can be connected to a vacuum source.

Abstract: A new improved blood withdrawal apparatus and method for using same wherein the apparatus includes a test tube housing for receiving a blood sample test tube, a test tube penetration assembly with the test tube housing for penetrating the blood sample test tube, a valve mechanism in flow communication with the test tube penetration assembly and the test tube housing for regulating the flow of blood to the test tube penetration assembly, and a connection assembly with the valve mechanism for connecting the valve mechanism in flow communication with an intravenous catheter positioned in the vein of the subject.

Abstract: An intravascular catheter or cannula assembly including a catheter device having a catheter tube and a catheter hub, and a cannula device having a cannula and a cannula hub. A cannula positioning rod is securely mounted on the cannula hub through a fixing member. A stopper is securely mounted on the catheter hub to abut against the distal end of the rod, thereby regulating the projecting length of the cannula from the distal end of the catheter tube when the cannula is inserted into the catheter tube. A member having a groove is provided to prevent an axial movement of the cannula. The groove tightly and detachably receives the fixing member and the stopper thereby integrally holding the catheter device and the cannula device when they are assembled.

Abstract: A bone marrow method and apparatus as been devised for the efficient recovery of bone marrow from a donor or patient by inserting a pair of aspiration needles at the intended site of removal and, through connection with a pair of syringes, the pressure is regulated to bring about selective removal of bone marrow and sinusoidal blood through one of the aspiration needles while positively forcing an intravenous solution through the other of the aspiration needles to replace the bone marrow removed from the site. The bone marrow and sinusoidal blood are drawn into a chamber for mixture with another intravenous solution and thereafter forced into a collection bag. A disposable assembly is provided for ready interchangeable use in association with a fluid flow and valve control unit in carrying out the method and principles of the present invention.