Abstract: The present Invention relates to methods of treating subjects preparing to undergo surgery or methods of treating chronic disease and methods for reducing transfusions and a computer readable storage medium and a physical computing device for carrying out these methods which include: a) receiving a profile of said subject, in a physical computing device, including a level of hemoglobin of said subject, level of creatinine of said subject, and information regarding current or past history of disease; b) applying said level of hemoglobin and said level of creatinine, in said physical computing device, to determine level of transfusion risk; c) applying said information regarding current or past history of disease of said subject, in said physical computing device, to determine level of comorbidity; d) applying said level of hemoglobin, said level of transfusion risk, and said level of comorbidity, in said physical computing device, to determine a patient care plan.

Abstract: A biological sample detection device configured to acquire color information of an object of analysis without a deterioration in color extraction accuracy caused by incorrect extraction of color of a colored label and to measure the amount of liquid and determine the type of a sample, by analyzing the biological sample housed in a biological sample tube to which a colored label is attached, and determining whether or not a colored label is present on the biological sample tube; extracting the colored label determined to be present by the colored label presence determining process; removing the colored label from the color image and extracting a detection object region in the biological sample; and analyzing to acquire color information of the detection object region and analyze the liquid amount and the type of the biological sample.

Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.

Abstract: A deep learning-based volumetric image inference system and method are disclosed that uses 2D images that are sparsely captured by a standard wide-field fluorescence microscope at arbitrary axial positions within the sample volume. Through a recurrent convolutional neural network (RNN) (referred to herein as Recurrent-MZ), 2D fluorescence information from a few axial planes within the sample is explicitly incorporated to digitally reconstruct the sample volume over an extended depth-of-field. Using experiments on C. elegans and nanobead samples, Recurrent-MZ is demonstrated to increase the depth-of-field of a 63×/1.4 NA objective lens by approximately 50-fold, also providing a 30-fold reduction in the number of axial scans required to image the same sample volume. The generalization of this recurrent network for 3D imaging is further demonstrated by showing its resilience to varying imaging conditions, including e.g.

Abstract: The present invention relates to methods for diagnosing Respiratory Distress Syndrome of newborn.

Abstract: Sensors are configured to capture measurement data representing dissolved oxygen (DO) measurements of an environment and capacitance measurements of a medium of the environment. A memory includes computer-executable instructions. One or more processors are communicatively coupled to the one or more sensors and configured to execute the computer-executable instructions to carry out operations comprising: generating, using first measurement data captured by the one or more sensors, a model based on a relationship between the first set of DO measurements and the first set of capacitance measurements; receiving, from the one or more sensors, second measurement data; predicting, using the model and based on the new capacitance measurement, an expected DO measurement; determining whether to use the expected DO measurement or the new DO measurement; and controlling, a valve to cause the determined oxygen input amount to flow into the environment based on the expected DO measurement.

Abstract: Embodiments may include an automated method for evaluating a sepsis status associated with a blood sample obtained from an individual. Methods may include determining a standard deviation of monocyte volume associated with the blood sample. Methods may include determining a white blood cell count (WBC) associated with the blood sample. Methods may include evaluating, using a data processing module, the sepsis status associated with the blood sample. The data processing module may include a processor and a computer application. This computer application may cause the processor to compare the standard deviation of monocyte volume with a first cutoff value to provide a comparison. The computer application may cause the processor to compare the WBC to a second cutoff to provide a second comparison. The computer application may also cause the processor to evaluate the sepsis status associated with the blood sample based on the first comparison and the second comparison.

Abstract: In an approach to generating quantitatively assessed synthetic training data, one or more computer processors identify an initial plurality of clusters in a dataset utilizing a trained classification model and a plurality of associated hyperparameters, wherein the clusters have sufficient density to be represented in a calculated probability distribution. The one or more computer processors generate one or more synthetic data points for each identified cluster utilizing a corresponding calculated probability distribution. The one or more computer processors quantitatively assess the one or more generated synthetic data points.

Abstract: A database query processing system for blood donation tracking includes a database, a network interface circuit and a processing circuit. The database stores records for a plurality of blood donors, each record comprising an amount of a blood component the blood donor has donated. The processing circuit determines an amount of a blood component a first donor may donate based on records for a plurality of donations made by the first blood donor and based on a limit of an amount of blood component the donor may donate in a predetermined period of time. The processing circuit further receives a request for the amount of the blood component the first donor may donate and transmits the amount of the blood component the first donor may donate to the remote computing device.

Abstract: A method for image processing medical self-test results receives a digital image of a self-test result generated by a self-test device. The digital image is processed using generalized curve field transforms to extract relevant geometrical features of the digital image to create a transformed image that is not distorted by distortions of the digital image. The transformed image is displayed for use in a medical diagnosis.

Abstract: Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.

Abstract: A fluorescence lifetime sensor module comprises an opaque housing having a first chamber and a second chamber which are separated by a light barrier. An optical emitter is arranged in the first chamber and configured to emit through a first aperture. Emission of pulses of light of a specified wavelength is arranged to optically excite a fluorescent probe to be positioned in front of the sensor module. A detector is arranged in the second chamber and configured to detect through a second aperture received photons from the fluorescent probe. A measurement block is configured to determine respective difference values representative of an arrival time of one of the received photons with respect to the emission pulses. A histogram block is configured to accumulate the difference values in a histogram.

Abstract: A removable smartphone case is disclosed. The removable smartphone case includes a case body configured to receive a smartphone, a radio frequency (RF) front-end connected to the case body and including a semiconductor substrate and an antenna array including at least one transmit antenna configured to transmit radio waves below the skin surface of a person and a two-dimensional array of receive antennas configured to receive radio waves, the received radio waves including a reflected portion of the transmitted radio waves, wherein the semiconductor substrate includes circuits configured to generate signals in response to the received radio waves, wherein the signals correspond to an alignment of the antenna array relative to a vein below the skin surface of the person, and a communications interface configured to transmit digital data that corresponds to the signals generated by the semiconductor substrate from the removable smartphone case.

Abstract: A system, a method, and a computer program product for providing transparent access to multi-temperature data are disclosed. A query for accessing data is received. The query includes at least one selection parameter for retrieval of data. The data is stored in a plurality of partitions of a database system. Each partition has a maximum data value per column, a minimum data value per column, and a predetermined date associated with the data contained in the partition. A determination is made whether at least one selection parameter is greater than the minimum data value per column and less than the maximum data value per column. The queried data is retrieved from the partition using the predetermined date associated with the data contained in the partition.

Abstract: A signal processing device that processes a bio-signal includes a lock-in amplifier chip configured to output a frequency-modulated modulation signal and enable one or more light sources to be driven in response to the modulation signal, and a multiplexer configured to receive and multiplex light sensing signals output from the light sources and then measured by multiple light measurement units, and the lock-in amplifier chip sequentially demodulates the multiple light sensing signals transmitted through the multiplexer.

Abstract: Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.

Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.

Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.

Abstract: Disclosed are a cell evaluation device, a cell evaluation method, and a non-transitory computer readable recording medium recorded with a cell evaluation program capable of evaluating individual cells in a cell image obtained by imaging a cell group with high accuracy. The cell evaluation device includes an image acquisition unit 30 that acquires a cell image obtained by imaging a cell group; a cell evaluation unit 32 that specifies an evaluation target cell and peripheral cells around the evaluation target cell in the cell group and evaluates the evaluation target cell based on evaluation results of the peripheral cells.

Abstract: A multifunctional sensor including a substrate, a first sensing structure, a dielectric layer and a second sensing structure is provided. The first sensing structure is disposed on the substrate. The dielectric layer is disposed on the first sensing structure. The second sensing structure is disposed on the dielectric layer. The first sensing structure and the second sensing structure are located on the same side of the substrate.

Abstract: This invention relates to a method of evaluating the similarity of structural effects of solvents determining solvent reactivity and a system using the same, and more particularly to a novel evaluation method that is able to quantitatively measure the structural effect of a solvent having an influence on reactivity upon reaction of the solvent with a predetermined material and to a system using the same.

Abstract: Techniques are described for metadata-driven code generation to generate code for analyzing data from clinical trial(s). A code generation engine may generate program code based on metadata that is input to the engine. The metadata may describe the data to be input to the generated code, and one or more artifacts to be output by the generated code on execution. The metadata may also describe one or more operations to be performed on the input data and/or intermediate data. The metadata may include one or more of the following: inline code to be included in the generated code; references to stored code to be included in the generated code; and/or instructions to be run to generate the code. Artifact(s) may include, but are not limited to, reports such as tables, figures, and/or listings that describe the results of analyzing or otherwise processing the data by the generated program code.

Abstract: Disclosed is a method for predicting the optimal amounts of radiopharmaceutical and/or chemotherapy agents to administer to a patient, by determining the level of saturation of the therapeutic agents in the patient's cells. The method comprises measuring cellular incorporation of the candidate therapeutic agents in a target cell population on a cell-by-cell basis. The method is able to identify an optimal cocktail of therapeutic agents for treatment of a disease. A method of high-throughput drug discovery incorporating this method, and a 2-stage targeting method of treating a disease using this method are also disclosed.

Abstract: A pre-programmed non-feedback continuous feeding method based on mass balance of the substrate in the bioreactor for use in culture growth and maintenance is provided. The disclosed method does not rely on instrument, probe or operator feedback. The method provides an efficient and effective alternative to bolus feeding.

Abstract: A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue.

Abstract: A method for detecting decalibration of a device for analyzing particles, including irradiating particles with light, detecting light scattered from the particles, amplifying, digitizing and detecting the electric signal obtained in a plurality of digital channels corresponding to the intensity representing the particle size and monitoring the appearance of the Mie peak in the measured size-dependent frequency distribution and sending a report if the Mie peak deviates in a digital channel other than the digital standard channel belonging to it based on measurement settings. The electronic analyzing unit detecting a Mie peak in the measured particle size distribution and assigning it to a digital detection channel in a device for detecting the concentration of small particles in gas, with a sample tube, a light source, a detector detecting scattered light scattered on the particles, an analog amplifier, an analog-digital converter, an electronic analyzing unit and a display and operating unit.

Abstract: Various embodiments include solutions for in-process material characterization. Various particular embodiments include a computer-implemented method including: providing instructions for transmitting oscillating electromagnetic field signals to a material under test (MUT); obtaining a return signal associated with the transmitted oscillating electromagnetic field signals; comparing the return signal with the oscillating electromagnetic field signals to determine a difference in an aspect of the return signal and the aspect of the oscillating electromagnetic field signals; comparing the difference in the aspect to a predetermined threshold; and determining a characteristic of the MUT based upon the compared difference.

Abstract: The method of detecting leukemia involves placing a suspension of red blood cells in a reservoir having a capillary tube partially immersed in the reservoir. An alternating current is applied to electrodes (one in the reservoir, the other in the capillary tube) at different frequencies. The electrical resistance is measured at the interface between the electrode and the suspension in the capillary tube. At the same time, CCD (charge coupled device) cameras take images, both top views and side views. The electrical resistance will show a peak at a characteristic resonant frequency, which is different for cancerous blood cells than for normal blood cells. The CCD images will show a pattern of maximum repulsion from the electrode at the resonant frequency in the top view, and minimum height of blood cells on the side of the electrode at the resonant frequency in the side view.

Abstract: The present invention provides automatic gating methods that are useful to gate populations of interest in multidimensional data, wherein the populations of interest are only a subset of the populations identifiable in the data. The populations are modeled as a finite mixture of multivariate probability distributions, preferably normal or t distributions. The distribution parameters that provide a best fit of the model distribution to the data are estimated using an Expectation Maximization (EM) algorithm that further includes a dynamic neighborhood thresholding that enables gating of a subset of the clusters present in the data.

Abstract: An image-processing program for cell observation comprises obtaining a first image showing a plurality of cells in an observation region, the first image being captured by an imaging device, and a second image showing the observation region, the second image being captured by the imaging device a predetermined time before the first image is captured; selecting one cell as a cell of interest from the plurality of cells included in the first image; specifying cells on the periphery of the cell of interest as peripheral cells; calculating movement statistics of the peripheral cells with respect to the cell of interest on the basis of the amount of relative movement of the cell of interest and the peripheral cells in the first image and the second image; and outputting the calculated movement statistics of the peripheral cells.

Abstract: The invention relates to a flow cytometer system and method to apply a gain to data measurements to improve the display of the data measurements. The method for applying a gain to data detected in a flow cytometer, involves obtaining measurements from a detector in a flow cytometer, applying a gain to the measurements to produce shifted measurements to allow for improved display of the shifted measurements while maintaining the relationship between data points of the shifted measurements and displaying the shifted measurements on a display.

Abstract: The present invention provides automatic gating methods that are useful to gate populations of interest in multidimensional data, wherein the populations of interest are only a subset of the populations identifiable in the data. The populations are modeled as a finite mixture of multivariate probability distributions, preferably normal or t distributions. The distribution parameters that provide a best fit of the model distribution to the data are estimated using an Expectation Maximization (EM) algorithm that further includes a dynamic neighborhood thresholding that enables gating of a subset of the clusters present in the data.

Abstract: The present invention relates to an apparatus that uses shadow images of cells to continuously measure cell activity at a high processing rate in order to provide cell activity and cell number results. According to one embodiment of the present invention, instead of a highly experienced examiner or technician using a microscope, ELISA reader, etc. having to collect various cell activity measurements and cell numbers, the collection of said information can be automated so as to reduce cost and largely reduce errors in measurements through the development of computer software coupled with hardware using low cost and compact optoelectronic components and simple image processing techniques.

Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.

Abstract: Methods and apparatus are disclosed for correcting measurements received by applying a frequency-varying signal with a measuring device (e.g., a permittivity probe) to a population of living cells (e.g., contained in a bioreactor) and correcting measurement divergences using data acquired using an alternate analytical method (e.g., trypan blue exclusion). In one example, a method comprises receiving electrical property data for a first population of cells, the data obtained by applying a first frequency-varying signal to the population with a measuring device, receiving biological property data obtained using an alternate analytical technique, generating at least one value representative of the frequency dependence of the electrical property data, and determining a relationship between the biological property data and the representative value. In some examples, measurements of apoptosis are predicted using the electrical property data.

Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.

Abstract: The present invention provides a method of identifying sub-populations of cells in a cellular sample. Aspects of the method include categorizing cells of the cellular sample into at least a first and second population based on a first phenotypic property. The method may further include sub-categorizing each of the first and second population into sub-populations of cells based on a second and third phenotypic property, e.g., by using X detectable labels providing Y distinct signals, wherein X>Y, to identify sub-populations of cells in the cellular sample.

Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.

Abstract: A measuring method for nucleic acid samples is provided. The measuring method provides qualitative and quantitative analyses of different types of templates of divergent concentration ranges in one experiment. Hence, the same test panel of various assays may be performed in a single test slide.

Abstract: Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer.

Abstract: Disclosed is a method for predicting the optimal amounts of radiopharmaceutical and/or chemotherapy agents to administer to a patient, by determining the level of saturation of the therapeutic agents in the patient's cells. The method comprises measuring cellular incorporation of the candidate therapeutic agents in a target cell population on a cell-by-cell basis. The method is able to identify an optimal cocktail of therapeutic agents for treatment of a disease. A method of high-throughput drug discovery incorporating this method, and a 2-stage targeting method of treating a disease using this method are also disclosed.

Abstract: The present invention is a sample analyzing system, including a sample analyzer and a management apparatus connected to the sample analyzer via a communication network. The management apparatus includes: a first memory that stores a computer program for the sample analyzer and manual data which corresponds to a version of the computer program; a first communication device; and a first controller configured to transmit, via the first communication device to the sample analyzer, the computer program and the manual data corresponding to the version of the computer program stored in the first memory. The sample analyzer includes: a second communication device; a second memory that stores the computer program and the manual data received by the second communication device; and a second controller configured to execute the computer program stored in the second memory.

Abstract: The disclosure provides biomarkers of amyotrophic lateral sclerosis (ALS). The disclosure also provides various methods of using the biomarkers, including methods for diagnosis of ALS, methods of determining predisposition to ALS, methods of monitoring progression/regression of ALS, methods of assessing efficacy of compositions for treating ALS, methods of screening compositions for activity in modulating biomarkers of ALS, methods of treating ALS, as well as other methods based on biomarkers of ALS.

Abstract: Disclosed is a cell analyzer comprising: a measuring device that includes a collecting section configured to collect target cells in a specimen with a filter, and is configured to measure the target cells collected by the collecting section; and a data processing device configured to analyze the target cells based on measurement data obtained by the measuring device, wherein the cell analyzer is operable in a first mode of measuring a clinical specimen collected from a subject and a second mode of measuring a quality control specimen containing particles having size capturable by the filter; and the data processing device is programmed to acquire an amount of particles collected by the collecting section based on measurement data of the quality control specimen obtained in the second mode, and output an alarm when the amount of particles meets a predetermined condition.

Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

Abstract: In some aspects of the present disclosure, methods of detecting coincident sample events are provided. The methods include receiving a first set of signal data representing detected signals from a flow cytometer system; detecting, with a peak detection module, one or more peaks within the signal data; and cancelling, with a successive cancellation module, one or more individual sample events from the signal data at corresponding time indexes, wherein the cancellation of more than one individual sample event is successive. Devices and system related thereto are also provided.

Abstract: A system for assessing the microvascular fitness of a sample of stored red blood cells. The system has a network device having at least one network unit. The network unit has a single inlet and a single outlet for the sample and a plurality of microchannels. The plurality of microchannels receive the sample from the single inlet and drain the sample into the single outlet. The network unit includes an aspiration pressure means for providing movement of liquid sample through the at least one network unit. The system further includes an analysis unit that receives the network device therein. The analysis unit includes a sensor for capturing measurements related to the sample and a processor capable of comparing the captured measurements to measurements stored in a database of healthy red blood cells to determine the microvascular fitness of the stored red blood cells.

Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.

Abstract: Processes are described for provision of privacy-sensitive sample analysis results to a sample provider. The sample provider generates a cryptographic commitment encoding a secret value, r, and a sample identifier, s, associated with a sample container. The sample provider provides the commitment to an analysis provider in association with the sample container containing a sample for analysis. The analysis provider analyzes the sample to obtain a set of analysis results corresponding to the sample identifier, s, and generates a cryptographic pre-credential, ??, corresponding to the sample identifier, s. The pre-credential, ??, encodes the set of analysis results and the commitment. Completion of the pre-credential, ??, requires knowledge of the secret value, r, in the commitment.

Abstract: A whole blood collection system includes an automated pump/control unit and an accompanying disposable blood set. When combined and connected to a source of anticoagulant, these elements allow automatic priming of the blood set with anticoagulant and automatic collection of anticoagulated blood product according to three different collection modes. The unit's pump and the blood set are specially designed to cooperate during the collection process to assure that the collected product has a precise blood to anticoagulant ratio. During the collection procedure, the pump/control unit automatically collects data relating to the procedure. Additional data specifically identifying components of the blood set, such as the blood collection bag, along with identification data on the donor's registration form may be scanned into the pump/controller unit by a scanner associated with the unit; this facilitates positive sample identification and tracking.