Cell Count Or Shape Or Size Analysis (e.g., Blood Cell) Patents (Class 702/21)
  • Patent number: 11971346
    Abstract: A biological sample detection device configured to acquire color information of an object of analysis without a deterioration in color extraction accuracy caused by incorrect extraction of color of a colored label and to measure the amount of liquid and determine the type of a sample, by analyzing the biological sample housed in a biological sample tube to which a colored label is attached, and determining whether or not a colored label is present on the biological sample tube; extracting the colored label determined to be present by the colored label presence determining process; removing the colored label from the color image and extracting a detection object region in the biological sample; and analyzing to acquire color information of the detection object region and analyze the liquid amount and the type of the biological sample.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: April 30, 2024
    Assignee: HITACHI HIGH-TECH CORPORATION
    Inventors: Mayu Sao, Nobuyoshi Shimane, Fumiyasu Sato, Tomoto Kawamura
  • Patent number: 11940372
    Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.
    Type: Grant
    Filed: September 28, 2022
    Date of Patent: March 26, 2024
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Jonathan Lin, Matthew Bahr, Keegan Owsley
  • Patent number: 11915360
    Abstract: A deep learning-based volumetric image inference system and method are disclosed that uses 2D images that are sparsely captured by a standard wide-field fluorescence microscope at arbitrary axial positions within the sample volume. Through a recurrent convolutional neural network (RNN) (referred to herein as Recurrent-MZ), 2D fluorescence information from a few axial planes within the sample is explicitly incorporated to digitally reconstruct the sample volume over an extended depth-of-field. Using experiments on C. elegans and nanobead samples, Recurrent-MZ is demonstrated to increase the depth-of-field of a 63×/1.4 NA objective lens by approximately 50-fold, also providing a 30-fold reduction in the number of axial scans required to image the same sample volume. The generalization of this recurrent network for 3D imaging is further demonstrated by showing its resilience to varying imaging conditions, including e.g.
    Type: Grant
    Filed: October 19, 2021
    Date of Patent: February 27, 2024
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Aydogan Ozcan, Yair Rivenson, Luzhe Huang
  • Patent number: 11781980
    Abstract: The present invention relates to methods for diagnosing Respiratory Distress Syndrome of newborn.
    Type: Grant
    Filed: October 5, 2018
    Date of Patent: October 10, 2023
    Assignee: Sime Diagnostics Ltd.
    Inventors: Peter Schousboe, Henrik Verder
  • Patent number: 11662327
    Abstract: Sensors are configured to capture measurement data representing dissolved oxygen (DO) measurements of an environment and capacitance measurements of a medium of the environment. A memory includes computer-executable instructions. One or more processors are communicatively coupled to the one or more sensors and configured to execute the computer-executable instructions to carry out operations comprising: generating, using first measurement data captured by the one or more sensors, a model based on a relationship between the first set of DO measurements and the first set of capacitance measurements; receiving, from the one or more sensors, second measurement data; predicting, using the model and based on the new capacitance measurement, an expected DO measurement; determining whether to use the expected DO measurement or the new DO measurement; and controlling, a valve to cause the determined oxygen input amount to flow into the environment based on the expected DO measurement.
    Type: Grant
    Filed: July 22, 2021
    Date of Patent: May 30, 2023
    Assignee: Sanofi
    Inventors: Kai Hoeffner, Brooke Tam, Jonathan Wang
  • Patent number: 11644464
    Abstract: Embodiments may include an automated method for evaluating a sepsis status associated with a blood sample obtained from an individual. Methods may include determining a standard deviation of monocyte volume associated with the blood sample. Methods may include determining a white blood cell count (WBC) associated with the blood sample. Methods may include evaluating, using a data processing module, the sepsis status associated with the blood sample. The data processing module may include a processor and a computer application. This computer application may cause the processor to compare the standard deviation of monocyte volume with a first cutoff value to provide a comparison. The computer application may cause the processor to compare the WBC to a second cutoff to provide a second comparison. The computer application may also cause the processor to evaluate the sepsis status associated with the blood sample based on the first comparison and the second comparison.
    Type: Grant
    Filed: April 22, 2019
    Date of Patent: May 9, 2023
    Assignee: Beckman Coulter, Inc.
    Inventors: Robert T. Magari, Diana B. Careaga, Liliana M. Tejidor
  • Patent number: 11636390
    Abstract: In an approach to generating quantitatively assessed synthetic training data, one or more computer processors identify an initial plurality of clusters in a dataset utilizing a trained classification model and a plurality of associated hyperparameters, wherein the clusters have sufficient density to be represented in a calculated probability distribution. The one or more computer processors generate one or more synthetic data points for each identified cluster utilizing a corresponding calculated probability distribution. The one or more computer processors quantitatively assess the one or more generated synthetic data points.
    Type: Grant
    Filed: March 19, 2020
    Date of Patent: April 25, 2023
    Assignee: International Business Machines Corporation
    Inventors: Gabriele Ranco, Moises Noe Sanchez Garcia, Gordon Doyle
  • Patent number: 11481395
    Abstract: A database query processing system for blood donation tracking includes a database, a network interface circuit and a processing circuit. The database stores records for a plurality of blood donors, each record comprising an amount of a blood component the blood donor has donated. The processing circuit determines an amount of a blood component a first donor may donate based on records for a plurality of donations made by the first blood donor and based on a limit of an amount of blood component the donor may donate in a predetermined period of time. The processing circuit further receives a request for the amount of the blood component the first donor may donate and transmits the amount of the blood component the first donor may donate to the remote computing device.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: October 25, 2022
    Assignee: FENWAL, INC.
    Inventors: Kathleen Mickles, Dale Meixelsperger
  • Patent number: 11412931
    Abstract: A method for image processing medical self-test results receives a digital image of a self-test result generated by a self-test device. The digital image is processed using generalized curve field transforms to extract relevant geometrical features of the digital image to create a transformed image that is not distorted by distortions of the digital image. The transformed image is displayed for use in a medical diagnosis.
    Type: Grant
    Filed: November 17, 2020
    Date of Patent: August 16, 2022
    Assignee: RELIANT IMMUNE DIAGNOSTICS, INC.
    Inventors: Jovan Hutton Pulitzer, Henry Joseph Legere, III, Hans Kristian Sandberg
  • Patent number: 11257215
    Abstract: Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.
    Type: Grant
    Filed: January 31, 2020
    Date of Patent: February 22, 2022
    Assignee: Labrador Diagnostics LLC
    Inventor: Elizabeth A. Holmes
  • Patent number: 10871448
    Abstract: A fluorescence lifetime sensor module comprises an opaque housing having a first chamber and a second chamber which are separated by a light barrier. An optical emitter is arranged in the first chamber and configured to emit through a first aperture. Emission of pulses of light of a specified wavelength is arranged to optically excite a fluorescent probe to be positioned in front of the sensor module. A detector is arranged in the second chamber and configured to detect through a second aperture received photons from the fluorescent probe. A measurement block is configured to determine respective difference values representative of an arrival time of one of the received photons with respect to the emission pulses. A histogram block is configured to accumulate the difference values in a histogram.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: December 22, 2020
    Assignee: AMS AG
    Inventor: Martin Münzer
  • Patent number: 10856766
    Abstract: A removable smartphone case is disclosed. The removable smartphone case includes a case body configured to receive a smartphone, a radio frequency (RF) front-end connected to the case body and including a semiconductor substrate and an antenna array including at least one transmit antenna configured to transmit radio waves below the skin surface of a person and a two-dimensional array of receive antennas configured to receive radio waves, the received radio waves including a reflected portion of the transmitted radio waves, wherein the semiconductor substrate includes circuits configured to generate signals in response to the received radio waves, wherein the signals correspond to an alignment of the antenna array relative to a vein below the skin surface of the person, and a communications interface configured to transmit digital data that corresponds to the signals generated by the semiconductor substrate from the removable smartphone case.
    Type: Grant
    Filed: November 13, 2019
    Date of Patent: December 8, 2020
    Assignee: Movano Inc.
    Inventor: Michael A. Leabman
  • Patent number: 10838926
    Abstract: A system, a method, and a computer program product for providing transparent access to multi-temperature data are disclosed. A query for accessing data is received. The query includes at least one selection parameter for retrieval of data. The data is stored in a plurality of partitions of a database system. Each partition has a maximum data value per column, a minimum data value per column, and a predetermined date associated with the data contained in the partition. A determination is made whether at least one selection parameter is greater than the minimum data value per column and less than the maximum data value per column. The queried data is retrieved from the partition using the predetermined date associated with the data contained in the partition.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: November 17, 2020
    Assignee: SAP SE
    Inventors: Christian Bensberg, Christian Kraus, Axel Herbst, Stefan Elfner, Holger Schwedes, Heiko Gerwens
  • Patent number: 10627349
    Abstract: A signal processing device that processes a bio-signal includes a lock-in amplifier chip configured to output a frequency-modulated modulation signal and enable one or more light sources to be driven in response to the modulation signal, and a multiplexer configured to receive and multiplex light sensing signals output from the light sources and then measured by multiple light measurement units, and the lock-in amplifier chip sequentially demodulates the multiple light sensing signals transmitted through the multiplexer.
    Type: Grant
    Filed: November 13, 2018
    Date of Patent: April 21, 2020
    Assignee: OLIVE HEALTHCARE INC.
    Inventors: Sung Ho Han, Keun Sik No, Hee Sun Hong
  • Patent number: 10565705
    Abstract: Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: February 18, 2020
    Assignee: Theranos IP Company, LLC
    Inventor: Elizabeth A. Holmes
  • Patent number: 10527580
    Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.
    Type: Grant
    Filed: July 25, 2018
    Date of Patent: January 7, 2020
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: James A. Ball, Brian Reed
  • Patent number: 10456814
    Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.
    Type: Grant
    Filed: November 6, 2018
    Date of Patent: October 29, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: James A. Ball, Phil Waggoner, Scott Parker
  • Patent number: 10443029
    Abstract: Disclosed are a cell evaluation device, a cell evaluation method, and a non-transitory computer readable recording medium recorded with a cell evaluation program capable of evaluating individual cells in a cell image obtained by imaging a cell group with high accuracy. The cell evaluation device includes an image acquisition unit 30 that acquires a cell image obtained by imaging a cell group; a cell evaluation unit 32 that specifies an evaluation target cell and peripheral cells around the evaluation target cell in the cell group and evaluates the evaluation target cell based on evaluation results of the peripheral cells.
    Type: Grant
    Filed: November 29, 2016
    Date of Patent: October 15, 2019
    Assignee: FUJIFILM CORPORATION
    Inventor: Kenta Matsubara
  • Patent number: 10401200
    Abstract: A multifunctional sensor including a substrate, a first sensing structure, a dielectric layer and a second sensing structure is provided. The first sensing structure is disposed on the substrate. The dielectric layer is disposed on the first sensing structure. The second sensing structure is disposed on the dielectric layer. The first sensing structure and the second sensing structure are located on the same side of the substrate.
    Type: Grant
    Filed: August 16, 2017
    Date of Patent: September 3, 2019
    Assignee: Winbond Electronics Corp.
    Inventors: Ming-Chih Tsai, Yu-Hsuan Ho
  • Patent number: 10289807
    Abstract: This invention relates to a method of evaluating the similarity of structural effects of solvents determining solvent reactivity and a system using the same, and more particularly to a novel evaluation method that is able to quantitatively measure the structural effect of a solvent having an influence on reactivity upon reaction of the solvent with a predetermined material and to a system using the same.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: May 14, 2019
    Assignee: LG Chem, Ltd.
    Inventors: Seung-Yup Lee, Ji-Won Jeong, Mi-Ri Kim, Kyoung-Shil Oh, Kyoung-Hoon Kim
  • Patent number: 10152512
    Abstract: Techniques are described for metadata-driven code generation to generate code for analyzing data from clinical trial(s). A code generation engine may generate program code based on metadata that is input to the engine. The metadata may describe the data to be input to the generated code, and one or more artifacts to be output by the generated code on execution. The metadata may also describe one or more operations to be performed on the input data and/or intermediate data. The metadata may include one or more of the following: inline code to be included in the generated code; references to stored code to be included in the generated code; and/or instructions to be run to generate the code. Artifact(s) may include, but are not limited to, reports such as tables, figures, and/or listings that describe the results of analyzing or otherwise processing the data by the generated program code.
    Type: Grant
    Filed: October 27, 2017
    Date of Patent: December 11, 2018
    Assignee: Accenture Global Solutions Limited
    Inventor: Barry R. Cohen
  • Patent number: 9804167
    Abstract: Disclosed is a method for predicting the optimal amounts of radiopharmaceutical and/or chemotherapy agents to administer to a patient, by determining the level of saturation of the therapeutic agents in the patient's cells. The method comprises measuring cellular incorporation of the candidate therapeutic agents in a target cell population on a cell-by-cell basis. The method is able to identify an optimal cocktail of therapeutic agents for treatment of a disease. A method of high-throughput drug discovery incorporating this method, and a 2-stage targeting method of treating a disease using this method are also disclosed.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: October 31, 2017
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: John M. Akudugu, Venkata S. Neti, Roger W. Howell
  • Patent number: 9803166
    Abstract: A pre-programmed non-feedback continuous feeding method based on mass balance of the substrate in the bioreactor for use in culture growth and maintenance is provided. The disclosed method does not rely on instrument, probe or operator feedback. The method provides an efficient and effective alternative to bolus feeding.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: October 31, 2017
    Assignee: AMGEN INC.
    Inventors: Henry Lin, Jeremy Bezaire
  • Patent number: 9658214
    Abstract: A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue.
    Type: Grant
    Filed: July 19, 2013
    Date of Patent: May 23, 2017
    Assignee: NIHON KOHDEN CORPORATION
    Inventors: Takahiro Shioyama, Akane Suzuki, Hirotsugu Kubo, Sunao Takeda
  • Patent number: 9581494
    Abstract: A method for detecting decalibration of a device for analyzing particles, including irradiating particles with light, detecting light scattered from the particles, amplifying, digitizing and detecting the electric signal obtained in a plurality of digital channels corresponding to the intensity representing the particle size and monitoring the appearance of the Mie peak in the measured size-dependent frequency distribution and sending a report if the Mie peak deviates in a digital channel other than the digital standard channel belonging to it based on measurement settings. The electronic analyzing unit detecting a Mie peak in the measured particle size distribution and assigning it to a digital detection channel in a device for detecting the concentration of small particles in gas, with a sample tube, a light source, a detector detecting scattered light scattered on the particles, an analog amplifier, an analog-digital converter, an electronic analyzing unit and a display and operating unit.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: February 28, 2017
    Assignee: PALAS GmbH Partikel- und Lasermeβtechnik
    Inventors: Maximilian Weiβ, Leander Mölter
  • Patent number: 9465061
    Abstract: Various embodiments include solutions for in-process material characterization. Various particular embodiments include a computer-implemented method including: providing instructions for transmitting oscillating electromagnetic field signals to a material under test (MUT); obtaining a return signal associated with the transmitted oscillating electromagnetic field signals; comparing the return signal with the oscillating electromagnetic field signals to determine a difference in an aspect of the return signal and the aspect of the oscillating electromagnetic field signals; comparing the difference in the aspect to a predetermined threshold; and determining a characteristic of the MUT based upon the compared difference.
    Type: Grant
    Filed: May 14, 2013
    Date of Patent: October 11, 2016
    Assignee: TransTech Systems, Inc.
    Inventors: Donald D. Colosimo, Sarah E. Pluta
  • Patent number: 9310328
    Abstract: The method of detecting leukemia involves placing a suspension of red blood cells in a reservoir having a capillary tube partially immersed in the reservoir. An alternating current is applied to electrodes (one in the reservoir, the other in the capillary tube) at different frequencies. The electrical resistance is measured at the interface between the electrode and the suspension in the capillary tube. At the same time, CCD (charge coupled device) cameras take images, both top views and side views. The electrical resistance will show a peak at a characteristic resonant frequency, which is different for cancerous blood cells than for normal blood cells. The CCD images will show a pattern of maximum repulsion from the electrode at the resonant frequency in the top view, and minimum height of blood cells on the side of the electrode at the resonant frequency in the side view.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: April 12, 2016
    Assignee: KING ABDULAZIZ UNIVERSITY
    Inventors: Soliman Mahmoud Soliman Abdalla, Abdullah Yousif Obaid
  • Patent number: 9164022
    Abstract: The present invention provides automatic gating methods that are useful to gate populations of interest in multidimensional data, wherein the populations of interest are only a subset of the populations identifiable in the data. The populations are modeled as a finite mixture of multivariate probability distributions, preferably normal or t distributions. The distribution parameters that provide a best fit of the model distribution to the data are estimated using an Expectation Maximization (EM) algorithm that further includes a dynamic neighborhood thresholding that enables gating of a subset of the clusters present in the data.
    Type: Grant
    Filed: February 12, 2015
    Date of Patent: October 20, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: Yuanxin Zhu, Mengxiang Tang
  • Patent number: 9080935
    Abstract: An image-processing program for cell observation comprises obtaining a first image showing a plurality of cells in an observation region, the first image being captured by an imaging device, and a second image showing the observation region, the second image being captured by the imaging device a predetermined time before the first image is captured; selecting one cell as a cell of interest from the plurality of cells included in the first image; specifying cells on the periphery of the cell of interest as peripheral cells; calculating movement statistics of the peripheral cells with respect to the cell of interest on the basis of the amount of relative movement of the cell of interest and the peripheral cells in the first image and the second image; and outputting the calculated movement statistics of the peripheral cells.
    Type: Grant
    Filed: September 24, 2010
    Date of Patent: July 14, 2015
    Assignee: NIKON CORPORATION
    Inventors: Masafumi Mimura, Kei Ito
  • Patent number: 9025145
    Abstract: The invention relates to a flow cytometer system and method to apply a gain to data measurements to improve the display of the data measurements. The method for applying a gain to data detected in a flow cytometer, involves obtaining measurements from a detector in a flow cytometer, applying a gain to the measurements to produce shifted measurements to allow for improved display of the shifted measurements while maintaining the relationship between data points of the shifted measurements and displaying the shifted measurements on a display.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: May 5, 2015
    Assignee: Beckman Coulter Biomedical, LLC
    Inventor: Michael Adeeb Thomas
  • Patent number: 8990047
    Abstract: The present invention provides automatic gating methods that are useful to gate populations of interest in multidimensional data, wherein the populations of interest are only a subset of the populations identifiable in the data. The populations are modeled as a finite mixture of multivariate probability distributions, preferably normal or t distributions. The distribution parameters that provide a best fit of the model distribution to the data are estimated using an Expectation Maximization (EM) algorithm that further includes a dynamic neighborhood thresholding that enables gating of a subset of the clusters present in the data.
    Type: Grant
    Filed: March 21, 2011
    Date of Patent: March 24, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: Yuanxin Zhu, Mengxiang Tang
  • Publication number: 20150050684
    Abstract: The present invention relates to an apparatus that uses shadow images of cells to continuously measure cell activity at a high processing rate in order to provide cell activity and cell number results. According to one embodiment of the present invention, instead of a highly experienced examiner or technician using a microscope, ELISA reader, etc. having to collect various cell activity measurements and cell numbers, the collection of said information can be automated so as to reduce cost and largely reduce errors in measurements through the development of computer software coupled with hardware using low cost and compact optoelectronic components and simple image processing techniques.
    Type: Application
    Filed: December 27, 2012
    Publication date: February 19, 2015
    Inventors: Sung-Kyu Seo, Geon-Soo Jin, Un-Hwan Ha, Se-Hwan Paek, Seung-Pil Pack
  • Patent number: 8953866
    Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: February 10, 2015
    Assignee: Amnis Corporation
    Inventors: Thaddeus C. George, David A. Basiji, Brian E. Hall, William E. Ortyn, Michael J. Seo, Philip J. Morrissey, Cathleen A. Zimmerman
  • Publication number: 20150019140
    Abstract: Methods and apparatus are disclosed for correcting measurements received by applying a frequency-varying signal with a measuring device (e.g., a permittivity probe) to a population of living cells (e.g., contained in a bioreactor) and correcting measurement divergences using data acquired using an alternate analytical method (e.g., trypan blue exclusion). In one example, a method comprises receiving electrical property data for a first population of cells, the data obtained by applying a first frequency-varying signal to the population with a measuring device, receiving biological property data obtained using an alternate analytical technique, generating at least one value representative of the frequency dependence of the electrical property data, and determining a relationship between the biological property data and the representative value. In some examples, measurements of apoptosis are predicted using the electrical property data.
    Type: Application
    Filed: January 4, 2013
    Publication date: January 15, 2015
    Applicant: Bend Research, Inc.
    Inventors: Brandon J. Downey, Jeffery F. Breit, Lisa Graham, Adam S. Carroll
  • Patent number: 8935098
    Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.
    Type: Grant
    Filed: September 16, 2011
    Date of Patent: January 13, 2015
    Assignee: The Regents of the University of California
    Inventors: Dino Di Carlo, Daniel R. Gossett, Henry T. K. Tse
  • Publication number: 20140349313
    Abstract: The present invention provides a method of identifying sub-populations of cells in a cellular sample. Aspects of the method include categorizing cells of the cellular sample into at least a first and second population based on a first phenotypic property. The method may further include sub-categorizing each of the first and second population into sub-populations of cells based on a second and third phenotypic property, e.g., by using X detectable labels providing Y distinct signals, wherein X>Y, to identify sub-populations of cells in the cellular sample.
    Type: Application
    Filed: April 29, 2014
    Publication date: November 27, 2014
    Inventors: Robert Balderas, Mirko Corselli
  • Publication number: 20140343869
    Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.
    Type: Application
    Filed: May 19, 2014
    Publication date: November 20, 2014
    Applicant: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Rudolf Hulspas
  • Publication number: 20140342928
    Abstract: A measuring method for nucleic acid samples is provided. The measuring method provides qualitative and quantitative analyses of different types of templates of divergent concentration ranges in one experiment. Hence, the same test panel of various assays may be performed in a single test slide.
    Type: Application
    Filed: May 20, 2014
    Publication date: November 20, 2014
    Applicant: CrackerBio, Inc.
    Inventor: Chung-Fan Chiou
  • Patent number: 8889072
    Abstract: Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: November 18, 2014
    Assignee: Beckman Coulter, Inc.
    Inventors: Jeffrey W. Degeal, Charles S. Bay, Edward A. Stanton, Paul Barclay Purcell, George C. Malachowski
  • Patent number: 8874380
    Abstract: Disclosed is a method for predicting the optimal amounts of radiopharmaceutical and/or chemotherapy agents to administer to a patient, by determining the level of saturation of the therapeutic agents in the patient's cells. The method comprises measuring cellular incorporation of the candidate therapeutic agents in a target cell population on a cell-by-cell basis. The method is able to identify an optimal cocktail of therapeutic agents for treatment of a disease. A method of high-throughput drug discovery incorporating this method, and a 2-stage targeting method of treating a disease using this method are also disclosed.
    Type: Grant
    Filed: December 9, 2011
    Date of Patent: October 28, 2014
    Assignee: Rutgers, The State University of New Jersey
    Inventors: John M. Akudugu, Venkata S. V. P. Neti, Roger W. Howell
  • Patent number: 8868370
    Abstract: The present invention is a sample analyzing system, including a sample analyzer and a management apparatus connected to the sample analyzer via a communication network. The management apparatus includes: a first memory that stores a computer program for the sample analyzer and manual data which corresponds to a version of the computer program; a first communication device; and a first controller configured to transmit, via the first communication device to the sample analyzer, the computer program and the manual data corresponding to the version of the computer program stored in the first memory. The sample analyzer includes: a second communication device; a second memory that stores the computer program and the manual data received by the second communication device; and a second controller configured to execute the computer program stored in the second memory.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: October 21, 2014
    Assignee: Sysmex Corporation
    Inventors: Naoki Shindo, Yusuke Suga, Aya Konishi, Daigo Fukuma, Keisuke Kuwano
  • Publication number: 20140303228
    Abstract: The disclosure provides biomarkers of amyotrophic lateral sclerosis (ALS). The disclosure also provides various methods of using the biomarkers, including methods for diagnosis of ALS, methods of determining predisposition to ALS, methods of monitoring progression/regression of ALS, methods of assessing efficacy of compositions for treating ALS, methods of screening compositions for activity in modulating biomarkers of ALS, methods of treating ALS, as well as other methods based on biomarkers of ALS.
    Type: Application
    Filed: October 17, 2012
    Publication date: October 9, 2014
    Inventors: Kay A. Lawton, Meredith V. Brown, Bruce Neri, Rebecca Caffrey, Michael V. Milburn
  • Publication number: 20140297200
    Abstract: Disclosed is a cell analyzer comprising: a measuring device that includes a collecting section configured to collect target cells in a specimen with a filter, and is configured to measure the target cells collected by the collecting section; and a data processing device configured to analyze the target cells based on measurement data obtained by the measuring device, wherein the cell analyzer is operable in a first mode of measuring a clinical specimen collected from a subject and a second mode of measuring a quality control specimen containing particles having size capturable by the filter; and the data processing device is programmed to acquire an amount of particles collected by the collecting section based on measurement data of the quality control specimen obtained in the second mode, and output an alarm when the amount of particles meets a predetermined condition.
    Type: Application
    Filed: March 27, 2014
    Publication date: October 2, 2014
    Applicant: Sysmex Corporation
    Inventors: Ryuichiro EBI, Emi KANEMURA, Kohei MURAKAMI
  • Publication number: 20140287530
    Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
    Type: Application
    Filed: March 26, 2014
    Publication date: September 25, 2014
    Applicant: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED
    Inventors: Michael P. Caulfield, Richard E. Reitz, Shuguang Li, Gloria Kwangja Lee, Ronald Krauss, Patricia J. Blanche, W. Henry Benner, Earl Cornell
  • Publication number: 20140268102
    Abstract: In some aspects of the present disclosure, methods of detecting coincident sample events are provided. The methods include receiving a first set of signal data representing detected signals from a flow cytometer system; detecting, with a peak detection module, one or more peaks within the signal data; and cancelling, with a successive cancellation module, one or more individual sample events from the signal data at corresponding time indexes, wherein the cancellation of more than one individual sample event is successive. Devices and system related thereto are also provided.
    Type: Application
    Filed: December 20, 2013
    Publication date: September 18, 2014
    Inventor: Amish Shah
  • Patent number: 8828226
    Abstract: A system for assessing the microvascular fitness of a sample of stored red blood cells. The system has a network device having at least one network unit. The network unit has a single inlet and a single outlet for the sample and a plurality of microchannels. The plurality of microchannels receive the sample from the single inlet and drain the sample into the single outlet. The network unit includes an aspiration pressure means for providing movement of liquid sample through the at least one network unit. The system further includes an analysis unit that receives the network device therein. The analysis unit includes a sensor for capturing measurements related to the sample and a processor capable of comparing the captured measurements to measurements stored in a database of healthy red blood cells to determine the microvascular fitness of the stored red blood cells.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: September 9, 2014
    Assignee: The Trustees of Boston University
    Inventors: Sergey Shevkoplyas, Tatsuro Yoshida, Mark Bitensky
  • Patent number: 8824770
    Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: September 2, 2014
    Assignee: Amnis Corporation
    Inventors: Thaddeus C. George, David A. Basiji, Brian E. Hall, William E. Ortyn, Michael J. Seo, Philip J. Morrissey, Cathleen A. Zimmerman
  • Patent number: 8825555
    Abstract: Processes are described for provision of privacy-sensitive sample analysis results to a sample provider. The sample provider generates a cryptographic commitment encoding a secret value, r, and a sample identifier, s, associated with a sample container. The sample provider provides the commitment to an analysis provider in association with the sample container containing a sample for analysis. The analysis provider analyzes the sample to obtain a set of analysis results corresponding to the sample identifier, s, and generates a cryptographic pre-credential, ??, corresponding to the sample identifier, s. The pre-credential, ??, encodes the set of analysis results and the commitment. Completion of the pre-credential, ??, requires knowledge of the secret value, r, in the commitment.
    Type: Grant
    Filed: June 30, 2011
    Date of Patent: September 2, 2014
    Assignee: International Business Machines Corporation
    Inventors: Thomas R. Gross, Erich M. Ruetsche
  • Patent number: 8788213
    Abstract: Disclosed herein are methods and devices for sectioning cell colonies. Also disclosed are methods of purifying cell colonies. A method of sectioning cell colonies can include providing a cell colony on a culture plate comprising a known thickness; positioning a bottom of the culture plate using automated focus technology; and sectioning the cell colony into one or more pieces using a pattern of laser cutting lines. Devices for performing the method are also disclosed.
    Type: Grant
    Filed: January 12, 2010
    Date of Patent: July 22, 2014
    Assignee: Intrexon Corporation
    Inventors: Gary Bright, Kristi Hohenstein, Anuradha Soundararajan, Manfred Koller
  • Patent number: RE45315
    Abstract: A whole blood collection system includes an automated pump/control unit and an accompanying disposable blood set. When combined and connected to a source of anticoagulant, these elements allow automatic priming of the blood set with anticoagulant and automatic collection of anticoagulated blood product according to three different collection modes. The unit's pump and the blood set are specially designed to cooperate during the collection process to assure that the collected product has a precise blood to anticoagulant ratio. During the collection procedure, the pump/control unit automatically collects data relating to the procedure. Additional data specifically identifying components of the blood set, such as the blood collection bag, along with identification data on the donor's registration form may be scanned into the pump/controller unit by a scanner associated with the unit; this facilitates positive sample identification and tracking.
    Type: Grant
    Filed: June 10, 2004
    Date of Patent: December 30, 2014
    Assignee: Haemonetics Corporation
    Inventors: Ronald O. Gilcher, Jacques Chammas, Joseph M. Medberry, Gary R. Stacey