Cell Count Or Shape Or Size Analysis (e.g., Blood Cell) Patents (Class 702/21)
  • Patent number: 10627349
    Abstract: A signal processing device that processes a bio-signal includes a lock-in amplifier chip configured to output a frequency-modulated modulation signal and enable one or more light sources to be driven in response to the modulation signal, and a multiplexer configured to receive and multiplex light sensing signals output from the light sources and then measured by multiple light measurement units, and the lock-in amplifier chip sequentially demodulates the multiple light sensing signals transmitted through the multiplexer.
    Type: Grant
    Filed: November 13, 2018
    Date of Patent: April 21, 2020
    Assignee: OLIVE HEALTHCARE INC.
    Inventors: Sung Ho Han, Keun Sik No, Hee Sun Hong
  • Patent number: 10565705
    Abstract: Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: February 18, 2020
    Assignee: Theranos IP Company, LLC
    Inventor: Elizabeth A. Holmes
  • Patent number: 10527580
    Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.
    Type: Grant
    Filed: July 25, 2018
    Date of Patent: January 7, 2020
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: James A. Ball, Brian Reed
  • Patent number: 10456814
    Abstract: A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.
    Type: Grant
    Filed: November 6, 2018
    Date of Patent: October 29, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: James A. Ball, Phil Waggoner, Scott Parker
  • Patent number: 10443029
    Abstract: Disclosed are a cell evaluation device, a cell evaluation method, and a non-transitory computer readable recording medium recorded with a cell evaluation program capable of evaluating individual cells in a cell image obtained by imaging a cell group with high accuracy. The cell evaluation device includes an image acquisition unit 30 that acquires a cell image obtained by imaging a cell group; a cell evaluation unit 32 that specifies an evaluation target cell and peripheral cells around the evaluation target cell in the cell group and evaluates the evaluation target cell based on evaluation results of the peripheral cells.
    Type: Grant
    Filed: November 29, 2016
    Date of Patent: October 15, 2019
    Assignee: FUJIFILM CORPORATION
    Inventor: Kenta Matsubara
  • Patent number: 10401200
    Abstract: A multifunctional sensor including a substrate, a first sensing structure, a dielectric layer and a second sensing structure is provided. The first sensing structure is disposed on the substrate. The dielectric layer is disposed on the first sensing structure. The second sensing structure is disposed on the dielectric layer. The first sensing structure and the second sensing structure are located on the same side of the substrate.
    Type: Grant
    Filed: August 16, 2017
    Date of Patent: September 3, 2019
    Assignee: Winbond Electronics Corp.
    Inventors: Ming-Chih Tsai, Yu-Hsuan Ho
  • Patent number: 10289807
    Abstract: This invention relates to a method of evaluating the similarity of structural effects of solvents determining solvent reactivity and a system using the same, and more particularly to a novel evaluation method that is able to quantitatively measure the structural effect of a solvent having an influence on reactivity upon reaction of the solvent with a predetermined material and to a system using the same.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: May 14, 2019
    Assignee: LG Chem, Ltd.
    Inventors: Seung-Yup Lee, Ji-Won Jeong, Mi-Ri Kim, Kyoung-Shil Oh, Kyoung-Hoon Kim
  • Patent number: 10152512
    Abstract: Techniques are described for metadata-driven code generation to generate code for analyzing data from clinical trial(s). A code generation engine may generate program code based on metadata that is input to the engine. The metadata may describe the data to be input to the generated code, and one or more artifacts to be output by the generated code on execution. The metadata may also describe one or more operations to be performed on the input data and/or intermediate data. The metadata may include one or more of the following: inline code to be included in the generated code; references to stored code to be included in the generated code; and/or instructions to be run to generate the code. Artifact(s) may include, but are not limited to, reports such as tables, figures, and/or listings that describe the results of analyzing or otherwise processing the data by the generated program code.
    Type: Grant
    Filed: October 27, 2017
    Date of Patent: December 11, 2018
    Assignee: Accenture Global Solutions Limited
    Inventor: Barry R. Cohen
  • Patent number: 9803166
    Abstract: A pre-programmed non-feedback continuous feeding method based on mass balance of the substrate in the bioreactor for use in culture growth and maintenance is provided. The disclosed method does not rely on instrument, probe or operator feedback. The method provides an efficient and effective alternative to bolus feeding.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: October 31, 2017
    Assignee: AMGEN INC.
    Inventors: Henry Lin, Jeremy Bezaire
  • Patent number: 9804167
    Abstract: Disclosed is a method for predicting the optimal amounts of radiopharmaceutical and/or chemotherapy agents to administer to a patient, by determining the level of saturation of the therapeutic agents in the patient's cells. The method comprises measuring cellular incorporation of the candidate therapeutic agents in a target cell population on a cell-by-cell basis. The method is able to identify an optimal cocktail of therapeutic agents for treatment of a disease. A method of high-throughput drug discovery incorporating this method, and a 2-stage targeting method of treating a disease using this method are also disclosed.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: October 31, 2017
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: John M. Akudugu, Venkata S. Neti, Roger W. Howell
  • Patent number: 9658214
    Abstract: A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue.
    Type: Grant
    Filed: July 19, 2013
    Date of Patent: May 23, 2017
    Assignee: NIHON KOHDEN CORPORATION
    Inventors: Takahiro Shioyama, Akane Suzuki, Hirotsugu Kubo, Sunao Takeda
  • Patent number: 9581494
    Abstract: A method for detecting decalibration of a device for analyzing particles, including irradiating particles with light, detecting light scattered from the particles, amplifying, digitizing and detecting the electric signal obtained in a plurality of digital channels corresponding to the intensity representing the particle size and monitoring the appearance of the Mie peak in the measured size-dependent frequency distribution and sending a report if the Mie peak deviates in a digital channel other than the digital standard channel belonging to it based on measurement settings. The electronic analyzing unit detecting a Mie peak in the measured particle size distribution and assigning it to a digital detection channel in a device for detecting the concentration of small particles in gas, with a sample tube, a light source, a detector detecting scattered light scattered on the particles, an analog amplifier, an analog-digital converter, an electronic analyzing unit and a display and operating unit.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: February 28, 2017
    Assignee: PALAS GmbH Partikel- und Lasermeβtechnik
    Inventors: Maximilian Weiβ, Leander Mölter
  • Patent number: 9465061
    Abstract: Various embodiments include solutions for in-process material characterization. Various particular embodiments include a computer-implemented method including: providing instructions for transmitting oscillating electromagnetic field signals to a material under test (MUT); obtaining a return signal associated with the transmitted oscillating electromagnetic field signals; comparing the return signal with the oscillating electromagnetic field signals to determine a difference in an aspect of the return signal and the aspect of the oscillating electromagnetic field signals; comparing the difference in the aspect to a predetermined threshold; and determining a characteristic of the MUT based upon the compared difference.
    Type: Grant
    Filed: May 14, 2013
    Date of Patent: October 11, 2016
    Assignee: TransTech Systems, Inc.
    Inventors: Donald D. Colosimo, Sarah E. Pluta
  • Patent number: 9310328
    Abstract: The method of detecting leukemia involves placing a suspension of red blood cells in a reservoir having a capillary tube partially immersed in the reservoir. An alternating current is applied to electrodes (one in the reservoir, the other in the capillary tube) at different frequencies. The electrical resistance is measured at the interface between the electrode and the suspension in the capillary tube. At the same time, CCD (charge coupled device) cameras take images, both top views and side views. The electrical resistance will show a peak at a characteristic resonant frequency, which is different for cancerous blood cells than for normal blood cells. The CCD images will show a pattern of maximum repulsion from the electrode at the resonant frequency in the top view, and minimum height of blood cells on the side of the electrode at the resonant frequency in the side view.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: April 12, 2016
    Assignee: KING ABDULAZIZ UNIVERSITY
    Inventors: Soliman Mahmoud Soliman Abdalla, Abdullah Yousif Obaid
  • Patent number: 9164022
    Abstract: The present invention provides automatic gating methods that are useful to gate populations of interest in multidimensional data, wherein the populations of interest are only a subset of the populations identifiable in the data. The populations are modeled as a finite mixture of multivariate probability distributions, preferably normal or t distributions. The distribution parameters that provide a best fit of the model distribution to the data are estimated using an Expectation Maximization (EM) algorithm that further includes a dynamic neighborhood thresholding that enables gating of a subset of the clusters present in the data.
    Type: Grant
    Filed: February 12, 2015
    Date of Patent: October 20, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: Yuanxin Zhu, Mengxiang Tang
  • Patent number: 9080935
    Abstract: An image-processing program for cell observation comprises obtaining a first image showing a plurality of cells in an observation region, the first image being captured by an imaging device, and a second image showing the observation region, the second image being captured by the imaging device a predetermined time before the first image is captured; selecting one cell as a cell of interest from the plurality of cells included in the first image; specifying cells on the periphery of the cell of interest as peripheral cells; calculating movement statistics of the peripheral cells with respect to the cell of interest on the basis of the amount of relative movement of the cell of interest and the peripheral cells in the first image and the second image; and outputting the calculated movement statistics of the peripheral cells.
    Type: Grant
    Filed: September 24, 2010
    Date of Patent: July 14, 2015
    Assignee: NIKON CORPORATION
    Inventors: Masafumi Mimura, Kei Ito
  • Patent number: 9025145
    Abstract: The invention relates to a flow cytometer system and method to apply a gain to data measurements to improve the display of the data measurements. The method for applying a gain to data detected in a flow cytometer, involves obtaining measurements from a detector in a flow cytometer, applying a gain to the measurements to produce shifted measurements to allow for improved display of the shifted measurements while maintaining the relationship between data points of the shifted measurements and displaying the shifted measurements on a display.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: May 5, 2015
    Assignee: Beckman Coulter Biomedical, LLC
    Inventor: Michael Adeeb Thomas
  • Patent number: 8990047
    Abstract: The present invention provides automatic gating methods that are useful to gate populations of interest in multidimensional data, wherein the populations of interest are only a subset of the populations identifiable in the data. The populations are modeled as a finite mixture of multivariate probability distributions, preferably normal or t distributions. The distribution parameters that provide a best fit of the model distribution to the data are estimated using an Expectation Maximization (EM) algorithm that further includes a dynamic neighborhood thresholding that enables gating of a subset of the clusters present in the data.
    Type: Grant
    Filed: March 21, 2011
    Date of Patent: March 24, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: Yuanxin Zhu, Mengxiang Tang
  • Publication number: 20150050684
    Abstract: The present invention relates to an apparatus that uses shadow images of cells to continuously measure cell activity at a high processing rate in order to provide cell activity and cell number results. According to one embodiment of the present invention, instead of a highly experienced examiner or technician using a microscope, ELISA reader, etc. having to collect various cell activity measurements and cell numbers, the collection of said information can be automated so as to reduce cost and largely reduce errors in measurements through the development of computer software coupled with hardware using low cost and compact optoelectronic components and simple image processing techniques.
    Type: Application
    Filed: December 27, 2012
    Publication date: February 19, 2015
    Inventors: Sung-Kyu Seo, Geon-Soo Jin, Un-Hwan Ha, Se-Hwan Paek, Seung-Pil Pack
  • Patent number: 8953866
    Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: February 10, 2015
    Assignee: Amnis Corporation
    Inventors: Thaddeus C. George, David A. Basiji, Brian E. Hall, William E. Ortyn, Michael J. Seo, Philip J. Morrissey, Cathleen A. Zimmerman
  • Publication number: 20150019140
    Abstract: Methods and apparatus are disclosed for correcting measurements received by applying a frequency-varying signal with a measuring device (e.g., a permittivity probe) to a population of living cells (e.g., contained in a bioreactor) and correcting measurement divergences using data acquired using an alternate analytical method (e.g., trypan blue exclusion). In one example, a method comprises receiving electrical property data for a first population of cells, the data obtained by applying a first frequency-varying signal to the population with a measuring device, receiving biological property data obtained using an alternate analytical technique, generating at least one value representative of the frequency dependence of the electrical property data, and determining a relationship between the biological property data and the representative value. In some examples, measurements of apoptosis are predicted using the electrical property data.
    Type: Application
    Filed: January 4, 2013
    Publication date: January 15, 2015
    Applicant: Bend Research, Inc.
    Inventors: Brandon J. Downey, Jeffery F. Breit, Lisa Graham, Adam S. Carroll
  • Patent number: 8935098
    Abstract: A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.
    Type: Grant
    Filed: September 16, 2011
    Date of Patent: January 13, 2015
    Assignee: The Regents of the University of California
    Inventors: Dino Di Carlo, Daniel R. Gossett, Henry T. K. Tse
  • Publication number: 20140349313
    Abstract: The present invention provides a method of identifying sub-populations of cells in a cellular sample. Aspects of the method include categorizing cells of the cellular sample into at least a first and second population based on a first phenotypic property. The method may further include sub-categorizing each of the first and second population into sub-populations of cells based on a second and third phenotypic property, e.g., by using X detectable labels providing Y distinct signals, wherein X>Y, to identify sub-populations of cells in the cellular sample.
    Type: Application
    Filed: April 29, 2014
    Publication date: November 27, 2014
    Inventors: Robert Balderas, Mirko Corselli
  • Publication number: 20140343869
    Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.
    Type: Application
    Filed: May 19, 2014
    Publication date: November 20, 2014
    Applicant: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Rudolf Hulspas
  • Publication number: 20140342928
    Abstract: A measuring method for nucleic acid samples is provided. The measuring method provides qualitative and quantitative analyses of different types of templates of divergent concentration ranges in one experiment. Hence, the same test panel of various assays may be performed in a single test slide.
    Type: Application
    Filed: May 20, 2014
    Publication date: November 20, 2014
    Applicant: CrackerBio, Inc.
    Inventor: Chung-Fan Chiou
  • Patent number: 8889072
    Abstract: Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: November 18, 2014
    Assignee: Beckman Coulter, Inc.
    Inventors: Jeffrey W. Degeal, Charles S. Bay, Edward A. Stanton, Paul Barclay Purcell, George C. Malachowski
  • Patent number: 8874380
    Abstract: Disclosed is a method for predicting the optimal amounts of radiopharmaceutical and/or chemotherapy agents to administer to a patient, by determining the level of saturation of the therapeutic agents in the patient's cells. The method comprises measuring cellular incorporation of the candidate therapeutic agents in a target cell population on a cell-by-cell basis. The method is able to identify an optimal cocktail of therapeutic agents for treatment of a disease. A method of high-throughput drug discovery incorporating this method, and a 2-stage targeting method of treating a disease using this method are also disclosed.
    Type: Grant
    Filed: December 9, 2011
    Date of Patent: October 28, 2014
    Assignee: Rutgers, The State University of New Jersey
    Inventors: John M. Akudugu, Venkata S. V. P. Neti, Roger W. Howell
  • Patent number: 8868370
    Abstract: The present invention is a sample analyzing system, including a sample analyzer and a management apparatus connected to the sample analyzer via a communication network. The management apparatus includes: a first memory that stores a computer program for the sample analyzer and manual data which corresponds to a version of the computer program; a first communication device; and a first controller configured to transmit, via the first communication device to the sample analyzer, the computer program and the manual data corresponding to the version of the computer program stored in the first memory. The sample analyzer includes: a second communication device; a second memory that stores the computer program and the manual data received by the second communication device; and a second controller configured to execute the computer program stored in the second memory.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: October 21, 2014
    Assignee: Sysmex Corporation
    Inventors: Naoki Shindo, Yusuke Suga, Aya Konishi, Daigo Fukuma, Keisuke Kuwano
  • Publication number: 20140303228
    Abstract: The disclosure provides biomarkers of amyotrophic lateral sclerosis (ALS). The disclosure also provides various methods of using the biomarkers, including methods for diagnosis of ALS, methods of determining predisposition to ALS, methods of monitoring progression/regression of ALS, methods of assessing efficacy of compositions for treating ALS, methods of screening compositions for activity in modulating biomarkers of ALS, methods of treating ALS, as well as other methods based on biomarkers of ALS.
    Type: Application
    Filed: October 17, 2012
    Publication date: October 9, 2014
    Inventors: Kay A. Lawton, Meredith V. Brown, Bruce Neri, Rebecca Caffrey, Michael V. Milburn
  • Publication number: 20140297200
    Abstract: Disclosed is a cell analyzer comprising: a measuring device that includes a collecting section configured to collect target cells in a specimen with a filter, and is configured to measure the target cells collected by the collecting section; and a data processing device configured to analyze the target cells based on measurement data obtained by the measuring device, wherein the cell analyzer is operable in a first mode of measuring a clinical specimen collected from a subject and a second mode of measuring a quality control specimen containing particles having size capturable by the filter; and the data processing device is programmed to acquire an amount of particles collected by the collecting section based on measurement data of the quality control specimen obtained in the second mode, and output an alarm when the amount of particles meets a predetermined condition.
    Type: Application
    Filed: March 27, 2014
    Publication date: October 2, 2014
    Applicant: Sysmex Corporation
    Inventors: Ryuichiro EBI, Emi KANEMURA, Kohei MURAKAMI
  • Publication number: 20140287530
    Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
    Type: Application
    Filed: March 26, 2014
    Publication date: September 25, 2014
    Applicant: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED
    Inventors: Michael P. Caulfield, Richard E. Reitz, Shuguang Li, Gloria Kwangja Lee, Ronald Krauss, Patricia J. Blanche, W. Henry Benner, Earl Cornell
  • Publication number: 20140268102
    Abstract: In some aspects of the present disclosure, methods of detecting coincident sample events are provided. The methods include receiving a first set of signal data representing detected signals from a flow cytometer system; detecting, with a peak detection module, one or more peaks within the signal data; and cancelling, with a successive cancellation module, one or more individual sample events from the signal data at corresponding time indexes, wherein the cancellation of more than one individual sample event is successive. Devices and system related thereto are also provided.
    Type: Application
    Filed: December 20, 2013
    Publication date: September 18, 2014
    Inventor: Amish Shah
  • Patent number: 8828226
    Abstract: A system for assessing the microvascular fitness of a sample of stored red blood cells. The system has a network device having at least one network unit. The network unit has a single inlet and a single outlet for the sample and a plurality of microchannels. The plurality of microchannels receive the sample from the single inlet and drain the sample into the single outlet. The network unit includes an aspiration pressure means for providing movement of liquid sample through the at least one network unit. The system further includes an analysis unit that receives the network device therein. The analysis unit includes a sensor for capturing measurements related to the sample and a processor capable of comparing the captured measurements to measurements stored in a database of healthy red blood cells to determine the microvascular fitness of the stored red blood cells.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: September 9, 2014
    Assignee: The Trustees of Boston University
    Inventors: Sergey Shevkoplyas, Tatsuro Yoshida, Mark Bitensky
  • Patent number: 8825555
    Abstract: Processes are described for provision of privacy-sensitive sample analysis results to a sample provider. The sample provider generates a cryptographic commitment encoding a secret value, r, and a sample identifier, s, associated with a sample container. The sample provider provides the commitment to an analysis provider in association with the sample container containing a sample for analysis. The analysis provider analyzes the sample to obtain a set of analysis results corresponding to the sample identifier, s, and generates a cryptographic pre-credential, ??, corresponding to the sample identifier, s. The pre-credential, ??, encodes the set of analysis results and the commitment. Completion of the pre-credential, ??, requires knowledge of the secret value, r, in the commitment.
    Type: Grant
    Filed: June 30, 2011
    Date of Patent: September 2, 2014
    Assignee: International Business Machines Corporation
    Inventors: Thomas R. Gross, Erich M. Ruetsche
  • Patent number: 8824770
    Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: September 2, 2014
    Assignee: Amnis Corporation
    Inventors: Thaddeus C. George, David A. Basiji, Brian E. Hall, William E. Ortyn, Michael J. Seo, Philip J. Morrissey, Cathleen A. Zimmerman
  • Patent number: 8788213
    Abstract: Disclosed herein are methods and devices for sectioning cell colonies. Also disclosed are methods of purifying cell colonies. A method of sectioning cell colonies can include providing a cell colony on a culture plate comprising a known thickness; positioning a bottom of the culture plate using automated focus technology; and sectioning the cell colony into one or more pieces using a pattern of laser cutting lines. Devices for performing the method are also disclosed.
    Type: Grant
    Filed: January 12, 2010
    Date of Patent: July 22, 2014
    Assignee: Intrexon Corporation
    Inventors: Gary Bright, Kristi Hohenstein, Anuradha Soundararajan, Manfred Koller
  • Patent number: 8784307
    Abstract: The invention is a method and system for functional biomechanical analysis. The analysis assesses the biomechanical relationships of the spine and adjacent regions in a dynamic state based on a patient's chief complaint and clinical presentation. The analysis calculates a value of function or impact for a particular spinal region.
    Type: Grant
    Filed: January 23, 2008
    Date of Patent: July 22, 2014
    Inventors: Eric K. Groteke, Scott Miller
  • Publication number: 20140186874
    Abstract: Embodiments of the present invention encompass automated systems and methods for analyzing immature platelet parameters in an individual based on a biological sample obtained from blood of the individual. Exemplary techniques involve correlating aspects of direct current (DC) impedance, radiofrequency (RF) conductivity, and/or light measurement data obtained from the biological sample with an evaluation of immature platelet conditions in the individual.
    Type: Application
    Filed: December 27, 2013
    Publication date: July 3, 2014
    Applicant: Beckman Coulter, Inc.
    Inventors: Jiuliu Lu, Phaisit Chewputtanagul, Christophe Godefroy, Patricio Vidal, John Riley
  • Patent number: 8762074
    Abstract: The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the steepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.
    Type: Grant
    Filed: May 10, 2012
    Date of Patent: June 24, 2014
    Assignee: Sino-Cell Technologies
    Inventors: Shiaw-Min Hwang, Chi-Hsien Liu, Chao-Ling Yao, I-Ming Chu, Tzu-Bou Hsieh
  • Publication number: 20140172321
    Abstract: Embodiments of the present invention encompass automated systems and methods for predicting an acute leukemia sub-type of an individual diagnosed with acute leukemia based on a biological sample obtained from blood of the individual. Exemplary techniques involve correlating aspects of direct current (DC) impedance, radiofrequency (RF) conductivity, and/or light measurement data obtained from the biological sample with an acute leukemic sub-type of the individual.
    Type: Application
    Filed: August 13, 2013
    Publication date: June 19, 2014
    Applicant: Beckman Coulter, Inc.
    Inventor: Kyungja Han
  • Publication number: 20140162374
    Abstract: An immunodiagnostic test method includes holding a selection of immunological test elements or consumables in one or more containers attached to or positioned in the analyzer and providing random access to any test element therein. The container can hold multiple types of test elements in compartments or slots. Through sensing of a test element position in its slot, the detection mechanism of the invention provides for random access to multiple types of test elements in any sleeve and within a single sleeve, and provides efficient inventory control. The method increases the number of test element types that may be loaded onto an analyzer and maintains fast determination of inventory.
    Type: Application
    Filed: March 8, 2013
    Publication date: June 12, 2014
    Applicant: Ortho-Clinical Diagnostics, Inc.
    Inventors: Jens H. Jorgensen, Joseph Wycallis
  • Patent number: 8744165
    Abstract: A stain separation system of digital pathology images that performs transforming a digital image from a first color domain to an optical domain to form an optical domain image (ODI), identifying a plane containing two or more basis vector which contain the pixels of the ODI, determining a plurality of orthogonal vector within the identified plane, forming a histogram of the digital image represented by the orthogonal vectors and determining one or more final stain vectors by searching for candidate vectors in the plane that minimize a cost function of the histogram.
    Type: Grant
    Filed: July 19, 2012
    Date of Patent: June 3, 2014
    Assignee: Sony Corporation
    Inventors: Ming-Chang Liu, Mark Robertson
  • Patent number: 8731845
    Abstract: The present invention relates to methods for identifying and optionally quantifying stained cells in a cell or tissue sample and determining expression level of a target, as well as methods for determining staining intensity and/or staining quality of a stained sample, calibrating digital imaging apparatus using the methods, as well as systems and a computer readable mediums therefore.
    Type: Grant
    Filed: August 14, 2009
    Date of Patent: May 20, 2014
    Assignee: Visionpharm A/S
    Inventors: Michael Grunkin, Niels Tækker Foged
  • Patent number: 8731844
    Abstract: The invention provides a system and method for selecting an optimal multimarker reagent combination for the identification and/or quantification of molecules in or on cells with or without reference to fluorescence or other properties of at least one fluorescent dye or other instrument-measurable atom or molecule associated directly or indirectly with the reagent combination. The method includes specifying, using a computer, a plurality of markers to be detected by a plurality of reagents, generating, using a computer, a plurality of reagent combinations comprising the plurality of reagents to detect the plurality of markers, wherein the reagent combinations are ranked according to at least one user-defined or system-defined criterion, and selecting the optimal reagent combination or providing a rank-ordered list of combinations.
    Type: Grant
    Filed: May 11, 2011
    Date of Patent: May 20, 2014
    Inventors: Leonore A. Herzenberg, David Rhodes Parks, Stephen Meehan, Wayne A. Moore
  • Patent number: 8712699
    Abstract: The present invention provides an agglutination judgment method includes a first step of collecting data of a transmitted light intensity from each of measuring points included in a predetermined region of the reaction pattern, a second step of calculating a parameter representing the dispersion of a transmitted light intensity in the region, and a third step of judging the agglutination or non-agglutination by comparing the parameter thus calculated with a predetermined reference value.
    Type: Grant
    Filed: May 14, 2009
    Date of Patent: April 29, 2014
    Assignee: Beckman Coulter, Inc.
    Inventor: Tadashi Haga
  • Patent number: 8676535
    Abstract: An analyzer which acquires first measured data relating to a first parameter and second measured data relating to a second parameter, the first and second measured data being obtained by measuring an analyte, forms a two-dimensional distribution chart that indicates a distribution of tangible components contained in the analyte based upon the first and second measured data, with the first and second parameters being set as two axes of the two-dimensional distribution chart, sets an area on the two-dimensional distribution chart, and forms a frequency distribution chart with respect to tangible components belonging to the area, with the first parameter being set as an axis of the frequency distribution chart is disclosed.
    Type: Grant
    Filed: June 27, 2005
    Date of Patent: March 18, 2014
    Assignee: Sysmex Corporation
    Inventor: Kimiyo Miyamoto
  • Patent number: 8658134
    Abstract: Methods of diagnosing Alzheimer's disease are provided. At least five methods of diagnostic measurements are presented: Method 1: Integrated score; Method 2: Average aggregate area per number of aggregates; Method 3: Cell migration analysis; Method 4; Fractal analysis; Method 5: Lacunarity Analysis. In certain embodiments, a sample of a subject's skin provides a network of fibroblasts that is imaged and a fractal dimension of the image is calculated. The fractal dimension can be compared to an aged-matched control (non-Alzheimer's) database to determine if the subject has Alzheimer's disease. The network of fibroblasts may be cultured in a matrix, for example in a protein mixture.
    Type: Grant
    Filed: October 2, 2010
    Date of Patent: February 25, 2014
    Assignee: Blanchette Rockefeller Neurosciences Institute
    Inventors: Florin Valentin Chirila, Tapan Kumar Khan, Daniel L. Alkon
  • Patent number: 8649981
    Abstract: A method and system for setting image analysis parameters to control image analysis operations. The method and system include collecting set of digital training images including a set of states for the set of digital training images. An objective function is defined to determine a relative quality of plural different parameter sets used for digital image analysis. Values for the plural different parameter sets that maximize (or minimize) the objective function are determined. The method and system increases a usability of high content screening technologies by reducing a required level of expertise required to configure digital image processing.
    Type: Grant
    Filed: September 24, 2010
    Date of Patent: February 11, 2014
    Assignee: Cellomics, Inc.
    Inventors: Michael A. Sipe, R. Terry Dunlay
  • Patent number: 8647859
    Abstract: An apparatus for analyzing bacteria is described that includes an analytic sample preparation section for preparing an analytic sample by treating a specimen so as to generate a morphological difference between Gram-negative bacteria and Gram-positive bacteria, a detector for detecting optical information from each particle contained in the analytic sample and an analyzing section for detecting Gram-positive bacteria contained on the basis of the detected optical information. A method for analyzing bacteria is also described.
    Type: Grant
    Filed: March 7, 2012
    Date of Patent: February 11, 2014
    Assignee: Sysmex Corporation
    Inventor: Yasuyuki Kawashima
  • Patent number: RE45315
    Abstract: A whole blood collection system includes an automated pump/control unit and an accompanying disposable blood set. When combined and connected to a source of anticoagulant, these elements allow automatic priming of the blood set with anticoagulant and automatic collection of anticoagulated blood product according to three different collection modes. The unit's pump and the blood set are specially designed to cooperate during the collection process to assure that the collected product has a precise blood to anticoagulant ratio. During the collection procedure, the pump/control unit automatically collects data relating to the procedure. Additional data specifically identifying components of the blood set, such as the blood collection bag, along with identification data on the donor's registration form may be scanned into the pump/controller unit by a scanner associated with the unit; this facilitates positive sample identification and tracking.
    Type: Grant
    Filed: June 10, 2004
    Date of Patent: December 30, 2014
    Assignee: Haemonetics Corporation
    Inventors: Ronald O. Gilcher, Jacques Chammas, Joseph M. Medberry, Gary R. Stacey