Abstract: Compositions and methods for conferring herbicide resistance to plant cells and bacterial cells are provided. The methods comprise transforming the cells with nucleotide sequences encoding herbicide resistance genes. In particular, herbicide resistance is conferred by expression of proteins with homology to decarboxylase enzymes. Compositions comprise transformed plants, plant tissues, and seeds, as well as transformed bacterial cells.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV427441. The invention thus relates to the plants, seeds and tissue cultures of the variety CV427441, and to methods for producing a corn plant produced by crossing a corn plant of variety CV427441 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV427441 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV427441.

Abstract: The present invention relates to compositions and methods for controlling nematode infestation of plants. In particular, the present invention provides vectors comprising sequences designed to control nematodes by RNA interference (RNAi) and transgenic plants transformed with such vectors.

Abstract: The invention relates to a method for producing plants in which expression of an insecticidal protein is regulated by a wound-induced promoter inducing local expression to the chimeric genes used in this method and the plants obtained thereby, and to the processes for obtaining resistance to insects feeding on plants by localized expression of an insecticidal protein induced on wounding of plants by insect feeding.

Abstract: The present invention provides a rice catalase B promoter for inducing expression specifically in at least one site of roots and stem apices. By using this promoter, it is possible to synthesize a resistance substance against nematodes or soil pathogens in roots; synthesize a substance promoting the development of roots in the roots; produce a gene recombinant plant in which expression of a selective marker in the redifferentiated plant body is limited to roots when the plant is young, so as to suppress the expression when the plant is matured; or the like. Further, the capability of expression in stem apices can be used to develop a technique for suppress gene expression in vigorously proliferating cells.

Abstract: The present invention is related to a recoverable block of function (RBF) system for controlling transgene escape and transgene containment in plants. The RBF-system comprises at least one blocking construct (BC), wherein the BC comprises as the blocking gene a nucleotide sequence or crtB gene encoding phytoene synthase, which is used alone or in combination with other BCs. The crtB gene expresses under the control of a germination specific promoter or a promoter constitutively repressed by a Recovering construct (RC) and blocks the germination of a transgenic seed. The blocked germination is recoverable by a user controlled intervention including addition of an effective amount of gibberellin and sugar and may involve so called recovering constructs (RCs), wherein the recovering gene may encode ent-copalyl diphosphate synthase or ent-kaurene synthase.

Abstract: Methods for the production of an apolipoprotein in plants are described. In one embodiment, the present invention provides a method for the expression of apolipoprotein in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5? to 3? direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant cells; and (ii) a nucleic acid sequence encoding an apolipoprotein polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and growing the plant cell into a mature plant capable of setting seed wherein the seed expresses apolipoprotein.

Abstract: The present invention relates to processes for preparing transformed plant cells or organisms by transforming a population of plant cells which comprises at least one marker protein having a direct or indirect toxic effect for said population, with at least one nucleic acid sequence to be inserted in combination with at least one compound, preferably a DNA construct, capable of reducing the expression, amount, activity and/or function of the marker protein, with the transformed plant cells having a growth advantage over nontransformed cells, due to the action of said compound.

Abstract: The invention provides polypeptides comprising inhibitor of apoptosis protein (IAP) family members, such as BmIAP initially derived from Bombyx mori BmN cells, and nucleic acids encoding them, and methods for making and using these compositions, including their use for inhibiting apoptosis.

Abstract: The present invention relates to transgenic plants for producing cellulose by a mechanism of autohydrolysis and a method producing soluble sugars using them, more particularly to transgenic plants transformed with recombinant cDNA coding cellulase, cellulose binding domain and chloroplast targeting peptide and a method producing soluble sugars using them. Transgenic plants and a method producing soluble sugars using them would be a highly cost-effective system for the production of soluble sugars on a large scale.

Abstract: Increased tolerance to drought in a plant is provided by introducing DNA expressing a bacterial cold shock protein.

Abstract: The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides plants and plant cells comprising of functional mammalian enzyme providing N-glycan biosynthesis that is normally not present in plants, for example mammalian ? 1,4-galactosyltransferase, said plants or plant cells additionally comprising at least a second mammalian protein or functional fragment thereof, for example a mammalian antibody, that is normally not present in plants.

Abstract: The invention relates to discovery of an isolated glyceraldehyde-3-phosphate dehydrogenase promoter in Pleurotus and a construct comprising the promoter of the invention operably linked to a heterologous transcribable polynucleotide molecule.

Abstract: Method for heterologous protein production in plant cell plastids comprising introducing into plant cells nucleic acid components that encode heterologous proteins under the control of promoters operative in plastids, vectors, host cells, plants and uses thereof.

Abstract: The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, and to methods of making these isolated blood proteins and to therapeutic compositions comprising them.

Abstract: The present invention relates generally to the use of plants as bioreactors for the production of molecules having useful properties such as inter alia polymers, metabolites, proteins, pharmaceuticals and nutraceuticals. More particularly, the present invention contemplates the use of grasses, and even more particularly C4 grasses, such as sugar-cane, for the production of a range of compounds such as, for example, polyhydroxyalkanoates, pHBA, vanillin, indigo, adipic acid, 2-phenylethanol, 1,3-propane-diol, sorbitol, fructan polymers and lactic acid as well as other products including, inter alia, other plastics, silks, carbohydrates, therapeutic and nutraceutic proteins and antibodies. The present invention further extends to transgenic plants and, in particular, transgenic C4 grass plants, capable of producing the compounds noted above and other products, and to methods for generating such plants.

Abstract: DNA encoding a herbicide metabolizing protein. Such DNA may, for example, be employed to produce herbicidally resistant plants.

Abstract: The present invention relates to a method for producing heterologous glycosylated proteins in non-animal eukaryotic cells such as in transformed bryophyte, yeast, ciliate or algae cells. In particular, the method relates to a method for producing glycosylated proteins comprising animal glycosylation patterns—comprising sialic acid residues—, such as pharmaceutical proteins for use in mammals, e.g. humans, in bryophyte cells such as those of Physcomitrella patens, the genetic material required therefore, such as DNA and RNA, vectors, host cells, methods of introducing genetic material there into, and uses thereof. Furthermore, the present invention relates to novel polypeptides and proteins obtained by the method according to the invention. Moreover, the present invention provides a method of producing sialic acid or CMP-sialic acid in a transformed non-mammalian eukaryotic cell, tissue or organism.

Abstract: Methods and constructs for the introduction of multiple genes into plants using a single transformation event are described. Constructs contain a single 5? promoter operably linked to DNA encoding a modified intein splicing unit. The splicing unit is expressed as a polyprotein and consists of a first protein fused to an intein fused to a second protein. The splicing unit has been engineered to promote excision of all non-essential components in the polyprotein but prevent the ligation reactions normally associated with protein splicing. Additional genetic elements encoding inteins and additional proteins can be fused in frame to the 5?-terminus of the coding region for the second protein to form a construct for expression of more than two proteins. A single 3? termination sequence, follows the last coding sequence. These methods and constructs are particularly useful for creating plants with stacked input traits and/or value added products.

Abstract: This invention relates to fertilizer and herbicide compositions. The present invention provides a composition for selective fertilization of a transgenic plant comprising a polypeptide which metabolizes a D-amino acid substrate; said composition comprising said D-amino acid substrate. Another aspect of the present invention provides a selective herbicide composition comprising a D-amino acid. Such a herbicide will inhibit or reduce the growth of plants which do not contain the appropriate D-amino acid metabolizing enzyme, while the growth of transgenic plants which are able to metabolize the D-amino acid will be unaffected or, more preferably, be increased or enhanced. Methods and means are also provided for selectively modulating the growth and stress tolerance such plants using D-amino acids.

Abstract: The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, Mortierella ramanniana DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinant techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.

Abstract: A method for obtaining improved fertility restorer lines for male sterile crop plants and a DNA construct for use in said method are disclosed. The invention relates to the simultaneous use of two different gene sequences encoding the same protein product, one being the naturally occurring wild type sequence and the other sequence being generated by modification of the wild type sequence for expression in crop plants by using codon degeneracy to avoid homology between the two sequences at the DNA and mRNA levels, each of the said sequences being placed under independent transcriptional control of different overlapping plant tissue-specific regulatory elements in the same DNA construct.

Abstract: Methods for screening mutations that affect the synthesis of plant small molecules or compounds capable of activating a mammalian nuclear receptor protein and systems for rapidly assigning functionality to genes that regulate plant secondary metabolism are provided.

Abstract: The invention relates to a novel transformation method for plants of the genus Allium. Plants transformed by the method are also provided. The method preferably involves an Agrobacterium tumefaciens-mediated transformation, involving the transformation of immature embryos as the explant source and wherein the method is carried out without passage through the callus phase.

Abstract: The present invention relates to a method for the preparation of transgenic plants, tissues or plant cells thereof having long-lasting resistant to the geminiviruses by a) “identification” or “selection” of a viral gene sequence encoding an amino acid sequence able to confer resistance against geminiviruses; b) mutagenesis or “choice” of the viral gene sequence so as to make it an ineffective target of the post-trascriptional silencing induced by infecting geminivirus; and c) insertion of the geminivirus mutated or chosen gene sequence obtained in step b) through a construct as described previously, in the plant, tissue or plant cell thereof.

Abstract: Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical.

Abstract: It is intended to provide a method of reducing the total amount of stored protein, to develop a technique required therefor, and to provide a plant and its seeds developed by the above method and a method of using such a plant and seeds. More specifically, a nucleic acid molecule containing a consecutive nucleic acid sequence of at least 15 in length which is complementary with a nucleic acid sequence encoding prolamin polypeptide or a nucleic acid sequence having a homology of at least about 70% to the complementary nucleic acid sequence having at least 15 nucleotide length. A method of reducing the expression dose of a protein in a seed of a plant which comprises: A) the step of providing the above-described nucleic acid molecule; B) the step of transferring the nucleic acid molecule into the cells of the plant; C) the step of re-differentiating the cells to construct a transgenic plant; and D) the step of obtaining seeds from the transgenic plant.

Abstract: The present invention relates to a DNA fragment specific to a cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NO: 1, a candidate polynucleotide (named orf456) associated with cytoplasmic male sterile pepper consisting of a 223rd to 678th nucleic acid of SEQ ID NO:1, and a polynucleotide of SEQ ID NO: 2. The DNA fragment specific to cytoplasmic male sterile pepper comprising a polynucleotide of SEQ ID NOs: 1 or 2 can be used for identifying cytoplasmic type between male sterile and male fertile pepper by the PCR method. In addition, hybrid pepper breeders/seed companies could detect impurities of the maintainer line within the CMS line, and by ensuring purity of the CMS line, a major source of contamination of the hybrid seeds is removed leading to obvious benefits for the seed industry and farmers.

Abstract: The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicotyledonous seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.

Abstract: A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.

Abstract: The present invention relates to vaccines that are made in transgenic soybeans for use in humans, animals of agricultural importance, pets, and wildlife. These vaccines are used as vaccines against viral, bacterial, fungal, parasitic or prion related diseases, cancer antigens, toxins, and autologous or self proteins. The transgenic soybeans of the instant invention also can be used for inducing tolerance to allergens or tolerance to autoimmune antigens, wherein an individual shows hypersensitivity to said allergen or has developed autoimmunity to autologous or self proteins, respectively. The invention also relates to prophylatically treating individuals and/or populations prior to showing hypersensitivity to allergens. Other aspects of the invention include using the transgenic soybeans as an oral contraceptive, and the expression of protein adjuvants in transgenic soybeans.

Abstract: The present invention provides a method of using cereal non-storage protein as fusion carrier to highly express small peptides in host endosperm cells. The method includes the steps of providing an endosperm-specific promoter and a DNA leading sequence encoding an endosperm-specific signal peptide; providing the gene of a non-storage protein as fusion carrier and an target gene; constructing a expression vector containing the promoter and DNA leading sequence, the gene of the fusion carrier, and a target gene; and expressing the expression vector in a host endosperm cell. Also provided in the invention are a vector constructed there from and the use thereof.

Abstract: The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.

Abstract: A process of the production of a product of interest in an F1 seed obtained by a hybridization of a first and a second transgenic parental plant, said hybridization generating a genetic endowment in said F1 seed for said production by combining in said F1 seed first and second partial genetic endowments of said first and second transgenic parental plants, followed by isolating said product of interest from said F1 seed or a seedling thereof.

Abstract: The present invention relates to nucleic acid molecules which code for a truncated alternan sucrase. The invention further relates to truncated alternan sucrases, to vectors, to host cells and to plant cells transformed with the nucleic acid molecules described, and to plants comprising these cells. The present invention furthermore relates to novel alternan polymers with advantageous properties and to methods for their production.

Abstract: The present invention provides a method for altering the tryptophan content of a plant by introducing and expressing an artificial polynucleotide sequence encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an artificial polynucleotide sequence encoding an anthranilate synthase, as well as human or animal food, seeds, and progeny derived from these plants, are also provided.

Abstract: The present invention relates to a method for transforming Allium species with a heterologous gene using Agrobacterium.

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include a novel class of EPSPS enzymes, designated Class III, and polynucleotides encoding such enzymes, vectors comprising those polynucleotides, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the Class III domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.

Abstract: The present invention relates to a transformed plant cell expressing ?-amyloid gene, which is known as a causal substance of senile dementia in human, a transformed plant and a preparation method of the same, more particularly, to a transformed plant cell expressing tandem repeats of ?-amyloid gene, a transformed plant produced by tissue-culturing the above cells and a preparation method of the same. The transformed plant of the present invention expresses multiple ?-amyloid protein, so that the plant can be effectively used for the preparation of an oral vaccine composition for the prevention of senile dementia in human.

Abstract: The invention relates to the soybean variety designated D5862173. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5862173. Also provided by the invention are tissue cultures of the soybean variety D5862173 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5862173 with itself or another soybean variety and plants produced by such methods.

Abstract: The invention relates to the soybean variety designated D5213471. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5213471. Also provided by the invention are tissue cultures of the soybean variety D5213471 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5213471 with itself or another soybean variety and plants produced by such methods.

Abstract: The present disclosure provides a method of producing transgenic plants which are resistant to at least one metal ion by transforming the plant with a recombinant DNA comprising a nucleic acid encoding a bacterial arsenic reductase under the control of a plant expressible promoter, and a nucleic acid encoding a nucleotide sequence encoding a phytochelatin biosynthetic enzyme under the control of a plant expressible promoter. The invention also relates a method of phytoremediation of a contaminated site by growing in the site a transgenic plant expressing a nucleic acid encoding a bacterial arsenate reductase and a nucleic acid encoding a phytochelatin biosynthetic enzyme.

Abstract: The invention relates to the soybean variety designated D5898941. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5898941. Also provided by the invention are tissue cultures of the soybean variety D5898941 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5898941 with itself or another soybean variety and plants produced by such methods.

Abstract: A system for expression of a heterologous polypeptide in a transgenic host cell is disclosed. The system is based upon a transgene comprising a eukaryotic promoter operably linked to a DNA sequence comprising, in the 5? to 3? direction, a DNA sequence complementary to a sequence encoding a heterologous polypeptide, a DNA sequence complementary to an internal ribosome entry site, and a DNA sequence corresponding to a 3? untranslated region of a positive strand single-stranded RNA virus. Following introduction of a stimulus, the host cell synthesizes an RNA molecule complementary to a recombinant RNA encoded by the transgene. The stimulus can be a positive strand single-stranded RNA virus or a nucleic acid thereof. Because the complement of the recombinant RNA comprises an internal ribosome entry site and a sequence encoding a heterologous polypeptide, the host cell can synthesize the heterologous polypeptide.

Abstract: The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

Abstract: The present invention provides systems and methods for producing a nucleic acid or protein in transgenic sprouted seedlings or sprouted seedlings engineered to transiently express a nucleic acid or protein of interest. The sprouted seedlings of the invention are grown in a contained, regulatable environment, wherein expression of a pharmaceutically active protein is controlled by an exogenously inducible promoter or a viral promoter. The sprouted seedlings may be eaten live or preferably harvested live to preserve the maximal biological activity of the nucleic acid or protein.

Abstract: A nucleic acid molecule is disclosed as containing a first nucleic acid sequence comprising a nucleotide sequence that encodes ?-zein protein, or a fragment thereof capable of directing and retaining a protein towards the endoplasmic reticulum (ER) of a plant cell; a second nucleic acid sequence containing a nucleotide sequence that encodes an amino acid sequence that is specifically cleavable by enzymatic or chemical means; and a third nucleic acid sequence containing the nucleotide sequence that encodes a peptide or protein of interest. Methods of using this nucleic acid molecule for transforming host plant cells and producing the peptide or protein of interest are also disclosed.

Abstract: The present invention provides systems and methods for producing pharmaceutically active proteins in transgenic sprouted seedlings. The transgenic sprouted seedlings of the invention are grown in a contained, regulatable environment, wherein expression of a pharmaceutically active protein is controlled by an exogenously inducible promoter. The transgenic sprouted seedlings may be eaten live or preferably harvested live to preserve the maximal biological activity of the pharmaceutically active protein.