Abstract: The present invention relates to isolated polypeptides having acetylxylan esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV112211. The invention thus relates to the plants, seeds and tissue cultures of the variety CV112211, and to methods for producing a corn plant produced by crossing a corn plant of variety CV112211 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV112211 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV112211.

Abstract: Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby.

Abstract: A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.

Abstract: The invention describes a highly improved, reproducible and a consistent method of transformation and regeneration that results in obtaining 12-15% transgenic plants. The present invention relates to a method of selecting genetically transformed Sunflower explants based on their ability to utilize Xylose as a sole carbohydrate source. Further disclosed is the nucleic acid sequence of the Xylose Isomerase gene, vector construction for incorporation of the selection marker gene and the process of Agrobacterium Mediated Transformation of target host plant with the vector comprising the gene encoding the enzyme Xylose isomerase under the functional combination of the heterologous regulatory sequences. Also disclosed is the method of selecting the putative transformants post transformation with the said vector that possess a metabolic advantage of utilizing Xylose as a sole carbon source.

Abstract: The invention provides transgenic corn seed, which expresses a gene encoding a double mutant of the E. coli glgC gene in endosperm plastids, wherein the mutant protein has a proline to aspartic acid substitution at amino acid 295 and a glutamic acid to lysine substitution at amino acid 296. The transgenic corn seed of the invention is characterized by enhanced levels of a number of amino acids and oil, when compared to isogenic corn seed, which does not express the transgene in an endosperm plastid. However, the amount of starch in the transgenic corn seed of the invention is decreased or unchanged when compared to the amount of starch in the isogenic control corn seed.

Abstract: A novel double haploid maize line designated PH17AW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PH17AW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH17AW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PH17AW or a trait conversion of PH17AW with another maize line. Inbred maize lines derived from double haploid maize line PH17AW, methods for producing other inbred maize lines derived from double haploid maize line PH17AW and the inbred maize lines and their parts derived by those methods.

Abstract: The invention relates to the soybean variety designated D5886524. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5886524. Also provided by the invention are tissue cultures of the soybean variety D5886524 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5886524 with itself or another soybean variety and plants produced by such methods.

Abstract: The present invention relates to methods for increasing the yield of a compound produced by an organism. More particularly, the present invention relates to methods for increasing the total or soluble carbohydrate content or sweetness or increasing the content of an endogenous carbohydrate of a plant tissue by producing a sugar-metabolizing enzyme that catalyzes the conversion of an endogenous sugar (one that is normally produced in the plant) to an alien sugar (one that is not normally produced in the plant at the same developmental stage). The invention also relates to plants and plant parts that produce a sugar-metabolizing enzyme to yield an alien sugar, with the consequence of higher total fermentable carbohydrate content, and to fermentable carbohydrates and other products derived therefrom.

Abstract: Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.

Abstract: This invention relates to a method of biocatalytically producing compounds that are commercially valuable but are detrimental to the host cells' development. The transgenic plants produced in accordance with the present invention comprise a novel controlled expression system comprising a promoter (2), a blocking sequence (a), and a structural gene (6), wherein the blocking sequence (a) is flanked by a pair of directly repeated site-specific recombination sequences (4). The blocking sequence (a) prevents expression of the structural gene (6) until removal of the blocking sequence (a) by site-specific recombinase activity encoded by a site-specific recombinase gene (12).

Abstract: A method for producing transgenic plants, including treating a target tissue using plasmolyzing media (PM) which contains 4% to 10% of sucrose and 100 ?M to 300 ?M of Acetosyringone (AS) and gold particles. The target tissue is infected by a bacterial suspension using a suitable strain and a suitable transformation vector. A PM containing 4% to 10% sucrose and 100 ?M to 300 ?M AS is treated for a period between 1 to 3 days. Cultivation is performed in a cultivation media in a dark condition at a temperature between 25° C. to 30° C. A non-selection media with an antibiotic is introduced. A selection media containing an active ingredient phosphinothricin (PPT) is introduced in a light condition at a temperature of between 25° C. to 30° C. in a sub culture for a period of between 3 weeks to 1 month. The putative transformant is regenerated and the number of copies of the transgenes is analyzed.

Abstract: The invention relates to the soybean variety designated D5726358. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5726358. Also provided by the invention are tissue cultures of the soybean variety D5726358 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5726358 with itself or another soybean variety and plants produced by such methods.

Abstract: The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.

Abstract: The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.

Abstract: The invention relates to the soybean variety designated D5852641. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5852641. Also provided by the invention are tissue cultures of the soybean variety D5852641 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5852641 with itself or another soybean variety and plants produced by such methods.

Abstract: The present invention provides multi-generational production methods for producing pharmaceutically active proteins and other proteins in corn. The present invention also provides methods for breeding corn capable of producing pharmaceutically active proteins or other proteins.

Abstract: The invention relates to the soybean variety designated D5638947. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5638947. Also provided by the invention are tissue cultures of the soybean variety D5638947 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5638947 with itself or another soybean variety and plants produced by such methods.

Abstract: The invention relates to stinging cells or isolated capsules and to their use in compositions and methods for efficient delivery of biologically active agents into a plant cell or plant tissue. The biologically active agent to be delivered by the methods of the present invention is selected from a nucleic acid, a peptide, a polypeptide, a plant hormone, an enzyme, an herbicidal agent, an anti-viral agent, an anti-bacterial agent and an anti-fungal agent. Particularly, the invention is related to compositions and methods for the efficient transformation of polynucleotide construct into a plant cell or tissue, to obtain transgenic plants.

Abstract: Transgenic guayule lines were created by constitutively expressing transgenes that encode for prenyltransferase and allylic diphosphate synthase. These new lines are important to rubber production because they result in plants that produce latex rubber which is lower in guayulin, a compound that produces a skin irritation in some animals. Controlling prenyltransferase production also permits the control of the production of resin by the plants, which has important industrial implications. Additionally, prenyltransferase manipulation can result in latex particles of different size, containing rubber of different molecular weight, which is also significant to industrial production. Although the elevated prenyltransferase activity in the transgenic lines resulted in shorter rubber molecules, the number of rubber molecules made by these transgenic lines was increased.

Abstract: The invention relates to genetically modified plants capable of producing a human recombinant protein Nerve Growth Factor, either in the form of pre-pro-protein or in the mature form and parts and differentiated and undifferentiated tissues thereof. The invention relates, also, to methods for the transformation of said plants in a transient way and methods for the transformation of said plants and tissues in a stable or transient way, methods for the recombinant protein purification from crude extract of proteins derived from plant tissue of said plants.

Abstract: The present inventions provide transgenic aloe plants and recombinant constructs for transforming aloe plants, aspects of which, may be applied to other monocots. The recombinant constructs may include one or more DNA sequences encoding mammalian proteins and at least one promoter capable of directing the expression of recombinant proteins in an aloe plant. The present inventions also provide methods for constructing and reproducing a transgenic aloe plant. The present inventions include methods for transfection of an aloe plant with several genes of interest simultaneously. The aloe plant production methods of the inventions may provide the potential to inexpensively and more safely mass-produce some biologically active compounds including biopharmaceuticals for disease therapy, diagnosis and prevention, and is more accessible to the less affluent countries. The aloe plant production methods may also produce proteins for cosmetics.

Abstract: The present invention relates to a process for the production of fine chemicals in a microorganism, a plant cell, a plant, a plant tissue or parts thereof by increasing or generating the biological activity of a ras-Like GTPase or the homologues thereof and growing the organism under conditions which permit the production of the fine chemicals in the organism. Preferred fine chemicals produced by the present invention include amino acids, carbohydrates, vitamins, fatty acids, and carotenoids.

Abstract: The invention relates to the soybean variety designated D5568543. Provided by the invention are the seeds, plants and derivatives of the soybean variety D5568543. Also provided by the invention are tissue cultures of the soybean variety D5568543 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety D5568543 with itself or another soybean variety and plants produced by such methods.

Abstract: Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: It is intended to provide by improving a known method of producing hyaluronic acid in a plant, a plant or a cultured plant cells which can produce hyaluronic acid at a lower cost and a further higher yield than before, a method of preparing the same, an expression vector for transformation, a method of producing hyaluronic acid using the plant or the cultured plant cells and the like. The method of producing hyaluronic acid comprising obtaining hyaluronic acid by co-expressing a protein with hyaluronic acid synthase activity and an exogenous protein with sugar-nucleotide synthase activity in a plant cell or a plant is provided.

Abstract: Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.

Abstract: The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.

Abstract: To provide a microorganism or a plant transformed with a ?-ionone ring-4-ketolase gene and/or ?-ionone ring-3-hydroxylase gene derived from Brevundimonas sp. strain SD-212. The ?-ionone ring-4-ketolase gene and ?-ionone ring-3-hydroxylase gene produced by Brevundimonas sp. strain SD-212 each have a high activity compared with those of known enzymes, and therefore microorganisms transformed with the genes encoding these enzymes can efficiently produce astaxanthin.

Abstract: A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.

Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract: Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a bioluminescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant.

Abstract: The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.

Abstract: The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.

Abstract: A novel inbred maize variety designated PHHEP and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize variety PHHEP with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHHEP through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred variety PHHEP or a trait conversion of PHHEP with another maize variety. Inbred maize varieties derived from inbred maize variety PHHEP, methods for producing other inbred maize varieties derived from inbred maize variety PHHEP and the inbred maize varieties and their parts derived by the use of those methods.

Abstract: Methods for producing a transgenic plant that is resistant to a weed control compound including introducing a nucleotide sequence encoding a coproporphyrinogen III oxidase of Escherichia coli into a plant cell, expressing the nucleotide sequence within the plant cell, and regenerating the plant cell into a transgenic plant and transgenic plants produced by such methods. Methods for controlling weeds including applying a weed control compound to an area containing transgenic plants containing a nucleotide sequence encoding a coproporphyrinogen III oxidase of Escherichia coli. Methods for selecting plants or plant cells resistant to a weed control compound including applying a weed control compound to transgenic plants or transgenic plant cells containing a nucleotide sequence encoding a coproporphyrinogen III oxidase of Escherichia coli.

Abstract: According to this invention, by introducing genes encoding hexulose-6-phosphate synthase and 6-phosphohexulose isomerase into a plant to have the genes expressed in the chloroplast of the plant, a transgenic plant having a pathway to assimilate formaldehyde through the Calvin cycle is provided. The transgenic plant according to this invention has resistance to formaldehyde and is capable of reducing the level of environmental formaldehyde significantly. Therefore, it is assumed that the transgenic plant according to this invention can be used to purify environmental condition, by placing it in a residence or in an office.

Abstract: The present invention provides a revolutionary photosynthetic ethanol production technology based on designer transgenic plants, algae, or plant cells. The designer plants, designer algae, and designer plant cells are created such that the endogenous photosynthesis regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic water splitting and proton gradient-coupled electron transport process are used for immediate synthesis of ethanol (CH3CH2OH) directly from carbon dioxide (CO2) and water (H2O). The ethanol production methods of the present invention completely eliminate the problem of recalcitrant lignocellulosics by bypassing the bottleneck problem of the biomass technology.

Abstract: Methods of expressing a molecule of interest in a plant are disclosed. One method comprises contacting roots of the plant in a solution comprising at least one Geminivirus based expression construct so as to allow the at least one Geminivirus based expression construct to be absorbed by the roots, the expression construct comprising a polynucleotide encoding the molecule of interest, and further the expression construct being capable of systemic symptomless spread in a plant host, thereby expressing a molecule of interest in a plant. Expression constructs capable of systemic symptomless spread in a host plant are also disclosed.

Abstract: The subject invention provides a plant cell culture for producing proteinaceous agents comprising a plant cell line stably transformed to express a transgene encoding a proteinaceous agent and a growth medium which supports the growth of said plant cell culture but which does not support the growth of Mycoplasmataceae and contains no materials of animal origin. The plant cell line is capable of being continuously passaged such that consistent transgene expression is maintained during passaging. The plant cell line is also capable of being cryopreserved such that consistent transgene expression is recovered upon recovery from cryopreservation.

Abstract: Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 6, 7, 9, 10, 12, 13, 15, 17, 18, 20, 21, or 23, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, 5, 8, 11, 14, 16, 19, or 22, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos.

Abstract: The present invention relates to an avian influenza vaccine of plant origin, prepared by transforming a plant with a surface protein of avian influenza virus, hemagglutinin (HA) or neuraminidase (NA), and a method for preparing the same. Further, the present invention relates to an oral vaccine for preventing avian influenza virus, of which mass-production conditions are established, and thus the produced transgenic plant can be more easily, safely, and economically used as a fodder additive. Furthermore, the present invention relates to a reagent for diagnosing avian influenza virus infection, prepared by isolating and purifying a recombinant antigen protein from the transgenic plant.

Abstract: Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.

Abstract: According to the invention, there is provided a novel soybean variety designated RJS34003. This invention thus relates to the seeds of soybean variety RJS34003, to the plants of soybean RJS34003 to plant parts of soybean variety RJS34003 and to methods for producing a soybean plant produced by crossing plants of the soybean variety RJS34003 with another soybean plant, using RJS34003 as either the male or the female parent.

Abstract: According to the invention, there is provided a novel soybean variety designated XB15M09. This invention thus relates to the seeds of soybean variety XB15M09, to the plants of soybean XB15M09 to plant parts of soybean variety XB15M09 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB15M09 with another soybean plant, using XB15M09 as either the male or the female parent.

Abstract: The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.

Abstract: The present invention provides methods of modifying in vivo mutagenesis or homeologous recombination in a eukaryote. The method of modifying in vivo mutagenesis involves transforming a eukaryote with a nucleotide sequence capable of expressing a wild-type prokaryotic MutS, MutL, MutH, MutU, NLS-MutS, NLS-MutL, NLS-MutH, NLS-MutU protein, or a combination thereof, and expressing the protein. A method of modifying recombination between homeologous chromosomes in an allopolyploid eukaryotic organism comprising, expressing a nucleotide sequence encoding prokaryotic NLS-MutS in combination with one or more than one of NLS-MutL, NLS-MutH, NLS-MutU, within a germ cell of the allopolyploid eukaryotic organism is also disclosed.

Abstract: Disclosed herein are zinc finger proteins that bind to target sites in a plant gamma-tocopherol methyl transferase (GMT) gene; compositions comprising these GMT-targeted zinc finger proteins and methods of making and using such zinc finger proteins. Also disclosed are methods for modulating the alpha-tocopherol content in various plant organs, particularly seeds, in transgenic plants.