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  • Modified luciferase protein
    Patent number: 8206961
    Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
    Type: Grant
    Filed: May 24, 2010
    Date of Patent: June 26, 2012
    Assignee: Marker Gene Technologies, Inc.
    Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
  • Gene encoding novel luciferase
    Patent number: 7871803
    Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.
    Type: Grant
    Filed: December 9, 2004
    Date of Patent: January 18, 2011
    Assignee: NEC Soft, Ltd.
    Inventor: Hiromi Takenaka
  • Modified luciola cruciata luciferase gene and protein
    Publication number: 20120028257
    Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
    Type: Application
    Filed: December 9, 2010
    Publication date: February 2, 2012
    Inventors: Ying Jiang, Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
  • Modified luciferase gene and protein
    Patent number: 7723502
    Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
    Type: Grant
    Filed: October 10, 2008
    Date of Patent: May 25, 2010
    Assignee: Marker Gene Technologies, Inc
    Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
  • Two secretory luciferases
    Patent number: 8367357
    Abstract: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
    Type: Grant
    Filed: October 23, 2009
    Date of Patent: February 5, 2013
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • METHOD FOR PRODUCING COMPLEX OF BIOTIN-LABELED CYPRIDINA (CYPRIDINA NOCTILUCA) LUCIFERASE WITH STREPTOAVIDIN AND METHOD FOR STABILIZING THE SAME
    Publication number: 20120077205
    Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.
    Type: Application
    Filed: December 3, 2010
    Publication date: March 29, 2012
    Inventors: Yoshihiro Ohmiya, Chun Wu, Satoru Ohgiya, Kosei Kawasaki
  • Two secretory luciferases
    Publication number: 20080090263
    Abstract: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
    Type: Application
    Filed: October 13, 2006
    Publication date: April 17, 2008
    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Luciferase showing orange luminescence
    Patent number: 10287556
    Abstract: A luciferase that includes an amino acid sequence in which a mutation is introduced into an amino acid sequence of a luciferase derived from Luciora kuroiwae. The luciferase catalyzes a luminescence reaction that generates luminescence having the maximum luminescent wavelength of 570 nm to 610 nm. The luminescence has an intensity at least 10 times higher than that of luminescence generated in a luminescence reaction catalyzed by a luciferase derived from Rhagophthalmus ohbai.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: May 14, 2019
    Assignee: OLYMPUS CORPORATION
    Inventor: Katsunori Ogo
  • THERMAL STABLE LUCIFERASE WITH IMPROVED RESISTANCE TO INHIBITION BY LUCIFERIN BREAK-DOWN PRODUCTS
    Publication number: 20190309342
    Abstract: Provided herein are inhibitor-resistant luciferase mutants, and methods of use thereof. In particular, luciferase mutants are provided that are thermal stable and exhibit improved stability in the presence of luciferin break-down products, such as dehydroluciferin. Further provided are assay systems comprising inhibitor-resistant luciferase mutants and amino acid sequences of the inhibitor-resistant luciferase mutants.
    Type: Application
    Filed: October 13, 2017
    Publication date: October 10, 2019
    Inventors: Ce Shi, Thomas Kirkland, Poncho Meisenheimer, Lance P. Encell, Mary Hall
  • Permuted and nonpermuted luciferase biosensors
    Patent number: 10077433
    Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.
    Type: Grant
    Filed: May 31, 2016
    Date of Patent: September 18, 2018
    Assignee: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
  • Modified luciferase gene and protein
    Patent number: 8450054
    Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
    Type: Grant
    Filed: December 9, 2010
    Date of Patent: May 28, 2013
    Assignee: Marker Gene Technologies, Inc.
    Inventors: Ying Jiang, Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
  • Dual-luciferase reporter system
    Patent number: 6143502
    Abstract: Plasmids and methods of use for assaying translational recoding are disclosed. The plasmids contain a constitutively expressed renilla (Renilla reniformis; sea pansy) luciferase gene, a polylinker for insertion of a selected DNA segment, and an out-of-frame firefly luciferase gene. Recoding is determined by monitoring luminescence of the firefly luciferase normalized to the luminescence of the renilla luciferase.
    Type: Grant
    Filed: March 31, 1999
    Date of Patent: November 7, 2000
    Assignee: University of Utah Research Foundation
    Inventors: Guido Grentzmann, Raymond F. Gesteland, John F. Atkins
  • Method for producing complex of biotin-labeled () luciferase with streptoavidin and method for stabilizing the same
    Patent number: 8465938
    Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.
    Type: Grant
    Filed: December 3, 2010
    Date of Patent: June 18, 2013
    Assignee: National Institute of Advanced Industrial Science and Technolgy
    Inventors: Yoshihiro Ohmiya, Chun Wu, Satoru Ohgiya, Kosei Kawasaki
  • Permuted and nonpermuted luciferase biosensors
    Patent number: 9359635
    Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.
    Type: Grant
    Filed: April 2, 2007
    Date of Patent: June 7, 2016
    Assignee: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
  • Mutant firefly luciferase
    Publication number: 20090081680
    Abstract: This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP/ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421st amino acid (glycine) of the amino acid sequence of the wild-type North American firefly (Photinus pyralis) luciferase has been substituted with a polar amino acid.
    Type: Application
    Filed: August 20, 2008
    Publication date: March 26, 2009
    Inventors: Hiroyuki Tsunoda, Hideki Kambara
  • Permuted and nonpermuted luciferase biosensors
    Publication number: 20110039257
    Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.
    Type: Application
    Filed: April 2, 2007
    Publication date: February 17, 2011
    Applicant: Promega Corporation
    Inventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
  • METHOD FOR PRODUCING COMPLEX OF BIOTIN-LABELED CYPRIDINA (CYPRIDINA NOCTILUCA) LUCIFERASE WITH STREPTOAVIDIN AND METHOD FOR STABILIZING THE SAME
    Publication number: 20080108097
    Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.
    Type: Application
    Filed: March 27, 2007
    Publication date: May 8, 2008
    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Yoshihiro Ohmiya, Chun Wu, Satoru Ohgiya, Kosei Kawasaki
  • STAR-WORM LUCIFERASE
    Publication number: 20140073031
    Abstract: The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.
    Type: Application
    Filed: September 13, 2013
    Publication date: March 13, 2014
    Applicants: OLYMPUS CORPORATION, PERAK STATE DEVELOPMENT CORPORATION, NIMURA GENETIC SOLUTIONS CO., LTD.
    Inventors: Ryutaro AKIYOSHI, Katsunori OGO, Hirobumi SUZUKI
  • Mutant luciferases
    Publication number: 20030166905
    Abstract: The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.
    Type: Application
    Filed: June 28, 2002
    Publication date: September 4, 2003
    Applicant: Promega Corporation
    Inventors: Keith V. Wood, Monika G. Wood
  • Mutant luciferases
    Patent number: 6552179
    Abstract: The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: April 22, 2003
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Monika G. Gruber
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