Patents Assigned to Amyris, Inc.
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Patent number: 9718741Abstract: A process of converting a carbon-carbon multiple bond to a cyclopropane ring, comprising the addition of a N-alkyl-N-nitroso compound to a mixture of alkene precursor, aqueous base and Pd(II)-catalyst, with the N-alkyl-N-nitroso compound obtained directly from an alkyl amine derivative, NaNO2 and an acid via phase separation of the N-alkyl-N-nitroso compound from the aqueous phase.Type: GrantFiled: October 24, 2014Date of Patent: August 1, 2017Assignees: Givaudan, S.A., Amyris, Inc.Inventors: Fridtjof Schroeder, Fabian Ruethi
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Patent number: 9701971Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, and a nuclease capable of causing a double-strand break near or within the genomic target site.Type: GrantFiled: February 11, 2014Date of Patent: July 11, 2017Assignee: AMYRIS, INC.Inventors: Zach Serber, Andrew Horwitz
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Patent number: 9670518Abstract: The present disclosure relates to the use of pantothenate compounds as a non-genetic switch for the production of heterologous acetyl-CoA derived (HACD) compounds in microbial host cells. The invention provides genetically modified microorganisms that are more stable when stored and initially cultured under reduced pantothenate concentrations, cell culture media having reduced concentrations of pantothenate compounds, and methods of producing HACD compounds using the cell culture media and the genetically engineered microorganisms of the invention.Type: GrantFiled: May 9, 2012Date of Patent: June 6, 2017Assignee: Amyris Inc.Inventor: Adam Meadows
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Patent number: 9611189Abstract: Methods and compositions for stabilization and subsequent hydrogenation of a microbial-derived immiscible olefin are described. The methods comprise providing a feed stream to the inlet of a reactor, wherein the feed stream comprises a microbial-derived immiscible olefin composition stabilized with a phenolic antioxidant which is a phenol derivative containing an unfused phenyl ring with one or more hydroxyl substituents. The methods further comprise contacting the feed stream with hydrogen in the presence of a hydrogenation catalyst at a temperature of about 20° C. or greater, and generating a product stream comprising a hydrogenated immiscible olefin composition. The microbial-derived immiscible olefin composition stabilized with the phenolic antioxidant remains stable over time, and its hydrogenation reaction time and process are improved.Type: GrantFiled: July 25, 2013Date of Patent: April 4, 2017Assignee: AMYRIS, INC.Inventors: Nicholas L. Ohler, Roberto Vazquez
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Publication number: 20170058299Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: ApplicationFiled: September 9, 2016Publication date: March 2, 2017Applicant: Amyris, Inc.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Patent number: 9534111Abstract: The present invention relates to a rubber composition including (A) at least one rubber component selected from the group consisting of a synthetic rubber and a natural rubber; (B) a polymer of farnesene; and (C) carbon black having an average particle size of from 5 to 100 nm, a content of the carbon black (C) in the rubber composition being from 20 to 100 parts by mass on the basis of 100 parts by mass of the rubber component (A).Type: GrantFiled: November 10, 2014Date of Patent: January 3, 2017Assignees: KURARAY CO., LTD., AMYRIS, INC.Inventors: Shigenao Kuwahara, Kei Hirata, Daisuke Koda
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Publication number: 20160347967Abstract: Provided is an aqueous emulsion including a block copolymer (I) containing a polymer block (a) composed of a structural unit derived from an aromatic vinyl compound and a polymer block (b) containing 1 to 100% by mass of a structural unit (b1) derived from farnesene and 99 to 0% by mass of a structural unit (b2) derived from a conjugated diene other than farnesene, with a mass ratio [(a)/(b)] of the polymer block (a) and the polymer block (b) being 5/95 to 70/30; a protective colloid (II); and water, a content of the protective colloid (II) being 3 to 35 parts by mass based on 100 parts by mass of the block copolymer (I).Type: ApplicationFiled: February 6, 2015Publication date: December 1, 2016Applicants: KURARAY CO., LTD., AMYRIS, INC.Inventor: Hiromitsu SASAKI
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Publication number: 20160312023Abstract: A thermoplastic elastomer composition comprising 100 parts by mass of a hydrogenated block copolymer (A) comprising a polymer block (a) consisting of a structural unit derived from an aromatic vinyl compound and a polymer block (b) comprising 1 to 100% by mass of a structural unit (b1) derived from farnesene and comprising 99 to 0% by mass of a structural unit (b2) derived from a conjugated diene other than farnesene, a mass ratio[(a)/(b)] of the polymer block (a) to the polymer block (b) being 1/99 to 70/30; and 5 to 300 parts by mass of a polar group-containing olefinic polymer (B), is excellent in flexibility and molding processability and capable of adhering to a ceramic, a metal, a synthetic resin, or the like even through a heat treatment at low temperatures (for example, 190° C. or lower) without requiring a primer treatment or the like.Type: ApplicationFiled: December 11, 2014Publication date: October 27, 2016Applicants: KURARAY CO., LTD., AMYRIS, INC.Inventors: Yusuke TANAKA, Hiromitsu SASAKI, Shota SUZUKI, Yosuke UEHARA
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Patent number: 9476065Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.Type: GrantFiled: December 19, 2014Date of Patent: October 25, 2016Assignee: AMYRIS, INC.Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
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Publication number: 20160297956Abstract: Provided is a polyolefin-based resin composition including a hydrogenated block copolymer (A) and a polyolefin-based resin (B), the hydrogenated block copolymer (A) being a hydrogenated product of a block copolymer including a polymer block (a) composed of a constitutional unit derived from an aromatic vinyl compound and a polymer block (b) containing 1 to 100% by mass of a constitutional unit (b1) derived from farnesene and 99 to 0% by mass of a constitutional unit (b2) derived from a conjugated diene other than farnesene; a mass ratio of the polymer block (a) and the polymer block (b) [(a)/(b)] being 1/99 to 55/45; 50 mol % or more of carbon-carbon double bonds in the polymer block (b) being hydrogenated; and a mass ratio of the polyolefin-based resin (B) and the hydrogenated block copolymer (A) [{polyolefin-based resin (B)}/{hydrogenated block copolymer (A)}] being 1/99 to 99/1.Type: ApplicationFiled: September 29, 2014Publication date: October 13, 2016Applicants: KURARAY CO., LTD., AMYRIS, INC.Inventors: Hiromitsu SASAKI, Yosuke UEHARA, Shota SUZUKI
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Publication number: 20160280615Abstract: A process of converting a carbon-carbon multiple bond to a cyclopropane ring, comprising the addition of a N-alkyl-N-nitroso compound to a mixture of alkene precursor, aqueous base and Pd(II)-catalyst, with the N-alkyl-N-nitroso compound obtained directly from an alkyl amine derivative, NaNO2 and an acid via phase separation of the N-alkyl-N-nitroso compound from the aqueous phase.Type: ApplicationFiled: October 24, 2014Publication date: September 29, 2016Applicants: Givaudan SA, Amyris Inc.Inventors: Fridtjof SCHROEDER, Fabian RUETHI
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Patent number: 9410214Abstract: Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.Type: GrantFiled: March 14, 2014Date of Patent: August 9, 2016Assignee: Amyris, Inc.Inventors: Kristy Michelle Hawkins, Tina Tipawan Mahatdejkul-Meadows, Adam Leon Meadows, Lauren Barbara Pickens, Anna Tai, Annie Ening Tsong
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Publication number: 20160200902Abstract: The present invention provides a rubber composition capable of producing a rubber molded article that is excellent in rolling resistance performance, steering stability, mechanical strength and abrasion resistance, and a tire obtained by using the rubber composition in at least a part thereof. The present invention relates to a rubber composition including (A) a rubber component including at least one rubber selected from the group consisting of a synthetic rubber and a natural rubber, (B) silica and (C) a modified polymer of farnesene obtained by introducing a functional group into a polymer of farnesene, in which a content of the silica (B) in the rubber composition is from 20 to 150 parts by mass on the basis of 100 parts by mass of the rubber component (A), and a content of the modified farnesene polymer (C) in the rubber composition is from 2 to 10 parts by mass on the basis of 100 parts by mass of the silica (B).Type: ApplicationFiled: August 11, 2014Publication date: July 14, 2016Applicants: KURARAY CO., LTD., AMYRIS, INC.Inventors: Daisuke KODA, Kei HIRATA
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Publication number: 20160177341Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.Type: ApplicationFiled: August 7, 2013Publication date: June 23, 2016Applicants: AMYRIS, INC., TOTAL MARKETING SERVICESInventors: Penelope R. CHUA, Adam MEADOWS
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Patent number: 9353201Abstract: The present invention relates to a hydrogenated block copolymer including a polymer block (A) containing a constitutional unit derived from an aromatic vinyl compound and a polymer block (B) containing from 1 to 100% by mass of a constitutional unit (b1) derived from farnesene and from 99 to 0% by mass of a constitutional unit (b2) derived from a conjugated diene other than the farnesene, in which 50 mol % or more of carbon-carbon double bonds in the polymer block (B) are hydrogenated.Type: GrantFiled: May 31, 2013Date of Patent: May 31, 2016Assignees: KURARAY CO., LTD., AMYRIS, INC.Inventors: Yosuke Uehara, Hiromitsu Sasaki
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Publication number: 20160108228Abstract: The thermoplastic elastomer composition of the present invention is a thermoplastic elastomer composition including a hydrogenated block copolymer (A) and a softening agent (B), the hydrogenated block copolymer (A) being a hydrogenated product of a block copolymer including a polymer block (a) composed of a constitutional unit derived from an aromatic vinyl compound and a polymer block (b) containing 1 to 100% by mass of a constitutional unit (b1) derived from farnesene and 99 to 0% by mass of a constitutional unit (b2) derived from a conjugated diene other than farnesene; a mass ratio of the polymer block (a) and the polymer block (b) [(a)/(b)] being 1/99 to 70/30; 50 mol % or more of carbon-carbon double bonds in the polymer block (b) being hydrogenated; and a content of the softening agent (B) being 20 to 2,000 parts by mass on the basis of 100 parts by mass of the hydrogenated block copolymer (A).Type: ApplicationFiled: September 29, 2014Publication date: April 21, 2016Applicants: KURARAY CO., LTD., AMYRIS, INC.Inventors: Hiromitsu SASAKI, Yosuke UEHARA, Masahiro KATO
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Publication number: 20160083750Abstract: Provided herein is a gaseous isoprene composition comprising isoprene, carbon dioxide and water, wherein the isoprene is in an amount between about 0.1% and about 15% by volume; wherein the carbon dioxide is in an amount between about 0.04% and about 35% by volume; wherein the water is in an amount greater than about 70% of its saturation amount. Also provided herein is a liquid isoprene composition comprising isoprene in an amount of at least 65% by weight and carbon dioxide in an amount between about 0.01% and about 1% by weight.Type: ApplicationFiled: December 2, 2015Publication date: March 24, 2016Applicant: AMYRIS, INC.Inventor: Derek MCPHEE
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Publication number: 20160053144Abstract: The present invention relates to a laminate including an adhesive layer that hardly suffers from adhesive transfer and exhibits a good adhesive strength, and a substrate layer, a protective film including the laminate, and a process for producing the laminate.Type: ApplicationFiled: March 12, 2014Publication date: February 25, 2016Applicants: KURARAY CO., LTD., AMYRIS, INC.Inventors: Hiromitsu SASAKI, Yosuke UEHARA, Yusuke TANAKA
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Patent number: 9233894Abstract: Provided herein is a gaseous isoprene composition comprising isoprene, carbon dioxide and water, wherein the isoprene is in an amount between about 0.1% and about 15% by volume; wherein the carbon dioxide is in an amount between about 0.04% and about 35% by volume; wherein the water is in an amount greater than about 70% of its saturation amount. Also provided herein is a liquid isoprene composition comprising isoprene in an amount of at least 65% by weight and carbon dioxide in an amount between about 0.01% and about 1% by weight.Type: GrantFiled: May 6, 2013Date of Patent: January 12, 2016Assignee: AMYRIS, INC.Inventor: Derek McPhee
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Patent number: 9228077Abstract: The present invention relates to a rubber composition including a rubber component (A), a farnesene polymer (B) and silica (C).Type: GrantFiled: February 18, 2013Date of Patent: January 5, 2016Assignees: KURARAY CO., LTD., AMYRIS, INC.Inventors: Daisuke Koda, Kei Hirata