Patents Assigned to Amyris, Inc.
  • Patent number: 9718741
    Abstract: A process of converting a carbon-carbon multiple bond to a cyclopropane ring, comprising the addition of a N-alkyl-N-nitroso compound to a mixture of alkene precursor, aqueous base and Pd(II)-catalyst, with the N-alkyl-N-nitroso compound obtained directly from an alkyl amine derivative, NaNO2 and an acid via phase separation of the N-alkyl-N-nitroso compound from the aqueous phase.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: August 1, 2017
    Assignees: Givaudan, S.A., Amyris, Inc.
    Inventors: Fridtjof Schroeder, Fabian Ruethi
  • Patent number: 9701971
    Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, and a nuclease capable of causing a double-strand break near or within the genomic target site.
    Type: Grant
    Filed: February 11, 2014
    Date of Patent: July 11, 2017
    Assignee: AMYRIS, INC.
    Inventors: Zach Serber, Andrew Horwitz
  • Patent number: 9670518
    Abstract: The present disclosure relates to the use of pantothenate compounds as a non-genetic switch for the production of heterologous acetyl-CoA derived (HACD) compounds in microbial host cells. The invention provides genetically modified microorganisms that are more stable when stored and initially cultured under reduced pantothenate concentrations, cell culture media having reduced concentrations of pantothenate compounds, and methods of producing HACD compounds using the cell culture media and the genetically engineered microorganisms of the invention.
    Type: Grant
    Filed: May 9, 2012
    Date of Patent: June 6, 2017
    Assignee: Amyris Inc.
    Inventor: Adam Meadows
  • Patent number: 9611189
    Abstract: Methods and compositions for stabilization and subsequent hydrogenation of a microbial-derived immiscible olefin are described. The methods comprise providing a feed stream to the inlet of a reactor, wherein the feed stream comprises a microbial-derived immiscible olefin composition stabilized with a phenolic antioxidant which is a phenol derivative containing an unfused phenyl ring with one or more hydroxyl substituents. The methods further comprise contacting the feed stream with hydrogen in the presence of a hydrogenation catalyst at a temperature of about 20° C. or greater, and generating a product stream comprising a hydrogenated immiscible olefin composition. The microbial-derived immiscible olefin composition stabilized with the phenolic antioxidant remains stable over time, and its hydrogenation reaction time and process are improved.
    Type: Grant
    Filed: July 25, 2013
    Date of Patent: April 4, 2017
    Assignee: AMYRIS, INC.
    Inventors: Nicholas L. Ohler, Roberto Vazquez
  • Publication number: 20170058299
    Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.
    Type: Application
    Filed: September 9, 2016
    Publication date: March 2, 2017
    Applicant: Amyris, Inc.
    Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
  • Patent number: 9534111
    Abstract: The present invention relates to a rubber composition including (A) at least one rubber component selected from the group consisting of a synthetic rubber and a natural rubber; (B) a polymer of farnesene; and (C) carbon black having an average particle size of from 5 to 100 nm, a content of the carbon black (C) in the rubber composition being from 20 to 100 parts by mass on the basis of 100 parts by mass of the rubber component (A).
    Type: Grant
    Filed: November 10, 2014
    Date of Patent: January 3, 2017
    Assignees: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Shigenao Kuwahara, Kei Hirata, Daisuke Koda
  • Publication number: 20160347967
    Abstract: Provided is an aqueous emulsion including a block copolymer (I) containing a polymer block (a) composed of a structural unit derived from an aromatic vinyl compound and a polymer block (b) containing 1 to 100% by mass of a structural unit (b1) derived from farnesene and 99 to 0% by mass of a structural unit (b2) derived from a conjugated diene other than farnesene, with a mass ratio [(a)/(b)] of the polymer block (a) and the polymer block (b) being 5/95 to 70/30; a protective colloid (II); and water, a content of the protective colloid (II) being 3 to 35 parts by mass based on 100 parts by mass of the block copolymer (I).
    Type: Application
    Filed: February 6, 2015
    Publication date: December 1, 2016
    Applicants: KURARAY CO., LTD., AMYRIS, INC.
    Inventor: Hiromitsu SASAKI
  • Publication number: 20160312023
    Abstract: A thermoplastic elastomer composition comprising 100 parts by mass of a hydrogenated block copolymer (A) comprising a polymer block (a) consisting of a structural unit derived from an aromatic vinyl compound and a polymer block (b) comprising 1 to 100% by mass of a structural unit (b1) derived from farnesene and comprising 99 to 0% by mass of a structural unit (b2) derived from a conjugated diene other than farnesene, a mass ratio[(a)/(b)] of the polymer block (a) to the polymer block (b) being 1/99 to 70/30; and 5 to 300 parts by mass of a polar group-containing olefinic polymer (B), is excellent in flexibility and molding processability and capable of adhering to a ceramic, a metal, a synthetic resin, or the like even through a heat treatment at low temperatures (for example, 190° C. or lower) without requiring a primer treatment or the like.
    Type: Application
    Filed: December 11, 2014
    Publication date: October 27, 2016
    Applicants: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Yusuke TANAKA, Hiromitsu SASAKI, Shota SUZUKI, Yosuke UEHARA
  • Patent number: 9476065
    Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, a nuclease capable of causing a break at the genomic target site, and a linear nucleic acid capable of homologous recombination with itself or with one or more additional linear nucleic acids contacted with the population of cells, whereupon said homologous recombination results in formation of a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker. In some embodiments, the methods further comprise selecting a host cell that expresses the selectable marker.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: October 25, 2016
    Assignee: AMYRIS, INC.
    Inventors: Andrew Horwitz, Kristy Michelle Hawkins, Max Schubert, Wayne Szeto
  • Publication number: 20160297956
    Abstract: Provided is a polyolefin-based resin composition including a hydrogenated block copolymer (A) and a polyolefin-based resin (B), the hydrogenated block copolymer (A) being a hydrogenated product of a block copolymer including a polymer block (a) composed of a constitutional unit derived from an aromatic vinyl compound and a polymer block (b) containing 1 to 100% by mass of a constitutional unit (b1) derived from farnesene and 99 to 0% by mass of a constitutional unit (b2) derived from a conjugated diene other than farnesene; a mass ratio of the polymer block (a) and the polymer block (b) [(a)/(b)] being 1/99 to 55/45; 50 mol % or more of carbon-carbon double bonds in the polymer block (b) being hydrogenated; and a mass ratio of the polyolefin-based resin (B) and the hydrogenated block copolymer (A) [{polyolefin-based resin (B)}/{hydrogenated block copolymer (A)}] being 1/99 to 99/1.
    Type: Application
    Filed: September 29, 2014
    Publication date: October 13, 2016
    Applicants: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Hiromitsu SASAKI, Yosuke UEHARA, Shota SUZUKI
  • Publication number: 20160280615
    Abstract: A process of converting a carbon-carbon multiple bond to a cyclopropane ring, comprising the addition of a N-alkyl-N-nitroso compound to a mixture of alkene precursor, aqueous base and Pd(II)-catalyst, with the N-alkyl-N-nitroso compound obtained directly from an alkyl amine derivative, NaNO2 and an acid via phase separation of the N-alkyl-N-nitroso compound from the aqueous phase.
    Type: Application
    Filed: October 24, 2014
    Publication date: September 29, 2016
    Applicants: Givaudan SA, Amyris Inc.
    Inventors: Fridtjof SCHROEDER, Fabian RUETHI
  • Patent number: 9410214
    Abstract: Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 9, 2016
    Assignee: Amyris, Inc.
    Inventors: Kristy Michelle Hawkins, Tina Tipawan Mahatdejkul-Meadows, Adam Leon Meadows, Lauren Barbara Pickens, Anna Tai, Annie Ening Tsong
  • Publication number: 20160200902
    Abstract: The present invention provides a rubber composition capable of producing a rubber molded article that is excellent in rolling resistance performance, steering stability, mechanical strength and abrasion resistance, and a tire obtained by using the rubber composition in at least a part thereof. The present invention relates to a rubber composition including (A) a rubber component including at least one rubber selected from the group consisting of a synthetic rubber and a natural rubber, (B) silica and (C) a modified polymer of farnesene obtained by introducing a functional group into a polymer of farnesene, in which a content of the silica (B) in the rubber composition is from 20 to 150 parts by mass on the basis of 100 parts by mass of the rubber component (A), and a content of the modified farnesene polymer (C) in the rubber composition is from 2 to 10 parts by mass on the basis of 100 parts by mass of the silica (B).
    Type: Application
    Filed: August 11, 2014
    Publication date: July 14, 2016
    Applicants: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Daisuke KODA, Kei HIRATA
  • Publication number: 20160177341
    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
    Type: Application
    Filed: August 7, 2013
    Publication date: June 23, 2016
    Applicants: AMYRIS, INC., TOTAL MARKETING SERVICES
    Inventors: Penelope R. CHUA, Adam MEADOWS
  • Patent number: 9353201
    Abstract: The present invention relates to a hydrogenated block copolymer including a polymer block (A) containing a constitutional unit derived from an aromatic vinyl compound and a polymer block (B) containing from 1 to 100% by mass of a constitutional unit (b1) derived from farnesene and from 99 to 0% by mass of a constitutional unit (b2) derived from a conjugated diene other than the farnesene, in which 50 mol % or more of carbon-carbon double bonds in the polymer block (B) are hydrogenated.
    Type: Grant
    Filed: May 31, 2013
    Date of Patent: May 31, 2016
    Assignees: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Yosuke Uehara, Hiromitsu Sasaki
  • Publication number: 20160108228
    Abstract: The thermoplastic elastomer composition of the present invention is a thermoplastic elastomer composition including a hydrogenated block copolymer (A) and a softening agent (B), the hydrogenated block copolymer (A) being a hydrogenated product of a block copolymer including a polymer block (a) composed of a constitutional unit derived from an aromatic vinyl compound and a polymer block (b) containing 1 to 100% by mass of a constitutional unit (b1) derived from farnesene and 99 to 0% by mass of a constitutional unit (b2) derived from a conjugated diene other than farnesene; a mass ratio of the polymer block (a) and the polymer block (b) [(a)/(b)] being 1/99 to 70/30; 50 mol % or more of carbon-carbon double bonds in the polymer block (b) being hydrogenated; and a content of the softening agent (B) being 20 to 2,000 parts by mass on the basis of 100 parts by mass of the hydrogenated block copolymer (A).
    Type: Application
    Filed: September 29, 2014
    Publication date: April 21, 2016
    Applicants: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Hiromitsu SASAKI, Yosuke UEHARA, Masahiro KATO
  • Publication number: 20160083750
    Abstract: Provided herein is a gaseous isoprene composition comprising isoprene, carbon dioxide and water, wherein the isoprene is in an amount between about 0.1% and about 15% by volume; wherein the carbon dioxide is in an amount between about 0.04% and about 35% by volume; wherein the water is in an amount greater than about 70% of its saturation amount. Also provided herein is a liquid isoprene composition comprising isoprene in an amount of at least 65% by weight and carbon dioxide in an amount between about 0.01% and about 1% by weight.
    Type: Application
    Filed: December 2, 2015
    Publication date: March 24, 2016
    Applicant: AMYRIS, INC.
    Inventor: Derek MCPHEE
  • Publication number: 20160053144
    Abstract: The present invention relates to a laminate including an adhesive layer that hardly suffers from adhesive transfer and exhibits a good adhesive strength, and a substrate layer, a protective film including the laminate, and a process for producing the laminate.
    Type: Application
    Filed: March 12, 2014
    Publication date: February 25, 2016
    Applicants: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Hiromitsu SASAKI, Yosuke UEHARA, Yusuke TANAKA
  • Patent number: 9233894
    Abstract: Provided herein is a gaseous isoprene composition comprising isoprene, carbon dioxide and water, wherein the isoprene is in an amount between about 0.1% and about 15% by volume; wherein the carbon dioxide is in an amount between about 0.04% and about 35% by volume; wherein the water is in an amount greater than about 70% of its saturation amount. Also provided herein is a liquid isoprene composition comprising isoprene in an amount of at least 65% by weight and carbon dioxide in an amount between about 0.01% and about 1% by weight.
    Type: Grant
    Filed: May 6, 2013
    Date of Patent: January 12, 2016
    Assignee: AMYRIS, INC.
    Inventor: Derek McPhee
  • Patent number: 9228077
    Abstract: The present invention relates to a rubber composition including a rubber component (A), a farnesene polymer (B) and silica (C).
    Type: Grant
    Filed: February 18, 2013
    Date of Patent: January 5, 2016
    Assignees: KURARAY CO., LTD., AMYRIS, INC.
    Inventors: Daisuke Koda, Kei Hirata