Patents Assigned to Amyris, Inc.
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Publication number: 20140221258Abstract: Provided herein are isoparaffins derived from hydrocarbon terpenes such as myrcene, ocimene and farnesene, and methods for making the same. In certain variations, the isoparaffins have utility as lubricant base stocks.Type: ApplicationFiled: February 13, 2012Publication date: August 7, 2014Applicant: AMYRIS, INC.Inventors: Nicholas Ohler, Karl Fisher, Shakeel Tirmizi
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Publication number: 20140213715Abstract: The present invention relates to a rubber composition including (A) at least one rubber component selected from the group consisting of a synthetic rubber and a natural rubber; (B) a polymer of farnesene; and (C) carbon black having an average particle size of from 5 to 100 nm, a content of the carbon black (C) in the rubber composition being from 20 to 100 parts by mass on the basis of 100 parts by mass of the rubber component (A).Type: ApplicationFiled: September 21, 2012Publication date: July 31, 2014Applicants: AMYRIS, INC., KURARAY CO., LTD.Inventors: Shigenao Kuwahara, Kei Hirata, Daisuke Koda
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Patent number: 8785542Abstract: The present invention relates to a rubber composition including (A) at least one rubber component selected from the group consisting of a synthetic rubber and a natural rubber; (B) a polymer of farnesene having a weight-average molecular weight of not less than 2,000 and less than 25,000; and (C) carbon black.Type: GrantFiled: September 21, 2012Date of Patent: July 22, 2014Assignees: Kuraray Co., Ltd., Amyris, Inc.Inventors: Shigenao Kuwahara, Kei Hirata, Daisuke Koda
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Publication number: 20140186942Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, and a nuclease capable of causing a double-strand break near or within the genomic target site.Type: ApplicationFiled: February 11, 2014Publication date: July 3, 2014Applicant: AMYRIS, INC.Inventors: Zach Serber, Andrew Horwitz
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Publication number: 20140154765Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: ApplicationFiled: October 24, 2013Publication date: June 5, 2014Applicant: AMYRIS, INC.Inventors: Timothy Stevens GARDNER, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
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Publication number: 20140155536Abstract: The present invention relates to a rubber composition including (A) at least one rubber component selected from the group consisting of a synthetic rubber and a natural rubber; (B) a polymer of farnesene having a weight-average molecular weight of not less than 2,000 and less than 25,000; and (C) carbon black.Type: ApplicationFiled: September 21, 2012Publication date: June 5, 2014Applicants: AMYRIS, INC., KURARAY CO., LTD.Inventors: Shigenao Kuwahara, Kei Hirata, Daisuke Koda
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Publication number: 20140148624Abstract: Provided herein are olefinic feedstocks derived from conjugated hydrocarbon terpenes (e.g., C10-C30 terpenes), methods for making the same, and methods for their use.Type: ApplicationFiled: February 13, 2012Publication date: May 29, 2014Applicant: AMYRIS, INC.Inventors: Nicholas Ohler, Karl Fisher, Jin Ki Hong
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Patent number: 8685737Abstract: Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, and a nuclease capable of causing a double-strand break near or within the genomic target site.Type: GrantFiled: April 27, 2012Date of Patent: April 1, 2014Assignee: Amyris, Inc.Inventors: Zach Serber, Andrew Horwitz
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Publication number: 20140057314Abstract: Provided herein are methods and compositions useful for detecting the production of compounds in a cell, for example, a microbial cell genetically modified to produce one or more such compounds at greater yield and/or with increased persistence compared to a parent microbial cell that is not genetically modified. In some embodiments, the methods comprise contacting a solution with a fluorescent dye that directly binds the recombinantly produced compound, wherein the solution comprises a plurality of cells recombinantly producing the compound; and detecting the fluorescent dye under spectral conditions suitable for the selective detection of the fluorescent dye bound to the recombinantly produced compound.Type: ApplicationFiled: May 10, 2012Publication date: February 27, 2014Applicant: AMYRIS, INC.Inventors: Jeff Ubersax, Lucas Frenz
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Patent number: 8603800Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: GrantFiled: January 28, 2013Date of Patent: December 10, 2013Assignee: AMYRIS, Inc.Inventors: Timothy Stevens Gardner, Kristy Michelle Hawkins, Adam Leon Meadows, Annie Ening Tsong, Yoseph Tsegaye
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Publication number: 20130316383Abstract: The present invention provides methods and compositions for monitoring cofactors and metabolites of a metabolic pathway of interest. The subject compositions and methods are particularly suited for monitoring the mevalonate pathway in a variety of cells. The invention also provides fermentation methods for the production of isoprenoids.Type: ApplicationFiled: April 25, 2013Publication date: November 28, 2013Applicant: AMYRIS, INC.Inventor: AMYRIS, INC.
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Publication number: 20130310617Abstract: Processes and systems for stabilization and subsequent hydrogenation of an immiscible olefin are described. In certain embodiments, the hydrogenation is conducted in a fixed bed reactor in presence of a hydrogenation catalyst.Type: ApplicationFiled: July 25, 2013Publication date: November 21, 2013Applicant: AMYRIS, INC.Inventors: Nicholas L. Ohler, Roberto Vazquez
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Publication number: 20130310615Abstract: Processes and systems for stabilization and subsequent hydrogenation of an immiscible olefin are described. In certain embodiments, the hydrogenation is conducted in a fixed bed reactor in presence of a hydrogenation catalyst.Type: ApplicationFiled: July 25, 2013Publication date: November 21, 2013Applicant: AMYRIS, INC.Inventors: Nicholas L. Ohler, Roberto Vazquez
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Patent number: 8586814Abstract: Provided herein are methods comprising catalytic dimerization of ?-farnesene to obtain squalane and/or isosqualane. Compositions comprising squalane and/or isosqualane are provided. In certain embodiments, squalane and isosqualane prepared by the methods provided herein can be useful for applications in cosmetic industry and/or in the lubricants industry.Type: GrantFiled: May 20, 2011Date of Patent: November 19, 2013Assignee: Amyris, Inc.Inventors: Karl Fisher, Susan Jessica Schofer, David B. Kanne
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Patent number: 8546136Abstract: The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides. The methods of the invention utilize circular nucleic acid vectors that comprise a DNA segment D flanked by an annealable linker sequence, annealable linker sequence pairs LA and LB, or annealable linker sequence/primer binding segment pairs LA and PB or PA and LB. Restriction endonuclease digestion of a plurality of vectors containing the DNA segments to be assembled generates a plurality of DNA fragments comprising the elements PA-D-LB, LA-D-LB, and LA-D-PB or D-LB, LA-D-LB, and LA-D. The sequences of annealable linker sequences LA and LB provide complementary termini to the DNA fragments, which are utilized in host cell mediated homologous recombination or together with promer binding segments PA and PB in a polymerase cycling assembly reaction for the ordered assembly of the various DNA segments into one or more assembled polynucleotides.Type: GrantFiled: March 26, 2012Date of Patent: October 1, 2013Assignee: Amyris, Inc.Inventors: Zach Serber, Raymond Lowe, Jeffrey A. Ubersax, Sunil S. Chandran
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Publication number: 20130236942Abstract: Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.Type: ApplicationFiled: January 28, 2013Publication date: September 12, 2013Applicant: AMYRIS, INC.Inventors: Timothy Stevens GARDNER, Kristy Michelle HAWKINS, Adam Leon MEADOWS, Annie Ening TSONG, Yoseph TSEGAYE
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Publication number: 20130221280Abstract: Provided herein is a gaseous isoprene composition comprising isoprene, carbon dioxide and water, wherein the isoprene is in an amount between about 0.1% and about 15% by volume; wherein the carbon dioxide is in an amount between about 0.04% and about 35% by volume; wherein the water is in an amount greater than about 70% of its saturation amount. Also provided herein is a liquid isoprene composition comprising isoprene in an amount of at least 65% by weight and carbon dioxide in an amount between about 0.01% and about 1% by weight.Type: ApplicationFiled: May 6, 2013Publication date: August 29, 2013Applicant: AMYRIS, INC.Inventor: Amyris, Inc.
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Patent number: 8519204Abstract: Processes and systems for stabilization and subsequent hydrogenation of an immiscible olefin are described. Methods of stabilizing a microbial-derived olefin composition are also described.Type: GrantFiled: April 2, 2010Date of Patent: August 27, 2013Assignee: Amyris, Inc.Inventors: Nicholas L. Ohler, Roberto Vazquez
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Patent number: 8492581Abstract: The present invention provides improved process for the sulfonation of hydroxyaromatics amenable to direct isolation of the sulfonylated hydroxyaromatics in their free-acid forms. The process allows for the recyclization of sulfuric acid and minimizes waste. The starting materials are from a renewal resource, e.g., biomass, and contain detectable 14C up to a 14C content of 0.0000000001% (one part per trillion). The products made include sulfonated catechol, disulfonated pyrogallol and sulfonated protocatechuic acid.Type: GrantFiled: August 20, 2010Date of Patent: July 23, 2013Assignee: Amyris, Inc.Inventors: John W Frost, Vu Bui
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Patent number: 8450080Abstract: The present invention provides methods and compositions for monitoring cofactors and metabolites of a metabolic pathway of interest. The subject compositions and methods are particularly suited for monitoring the mevalonate pathway in a variety of cells. The invention also provides fermentation methods for the production of isoprenoids.Type: GrantFiled: January 28, 2009Date of Patent: May 28, 2013Assignee: Amyris, Inc.Inventors: Sunil Bajad, Michael Leavell