Abstract: The present invention is related to the field of bio/chemical sampling, sensing, assays and applications. Particularly, the present invention is related to how to make the sampling/sensing/assay become simple to use, fast to results, highly sensitive, easy to use, using tiny sample volume (e.g. 0.5 uL or less), operated by a person without any professionals, reading by mobile-phone, or low cost, or a combination of them.
Abstract: Provided herein is a method and device for performing a homogeneous nucleic acid detection assay. The device can contain a pair of plates where one of the plates comprises (i) surface amplification surface; and (ii) target-specific nucleic acid probes that are immobilized on said amplification surface and that specifically binds to a part of the target nucleic acid; and the second plate comprises a sample contact area comprising a reagent storage site that comprises target-specific nucleic acid detection agents that specifically binds to another part of the target nucleic acid. In some embodiments, the device can be read without a washing unbound label from the surface of the device.
Type:
Application
Filed:
March 22, 2022
Publication date:
July 7, 2022
Applicant:
Essenlix Corporation
Inventors:
Stephen Y. Chou, Wei Ding, Ji Qi, Yufan Zhang, Ji Li
Abstract: The present invention provides devices, systems, and methods for rapid and easy-to-use in sample thermal cycling or temperature changes for the facilitation of reactions such as but not limited to PCR.
Type:
Grant
Filed:
January 15, 2021
Date of Patent:
June 28, 2022
Assignee:
Essenlix Corporation
Inventors:
Stephen Y. Chou, Wei Ding, Yufan Zhang, Hua Tan
Abstract: Disclosed is a method for correlating a biomarker in a non-blood bodily fluid with the same biomarker in the blood of an individual, including: measuring, in a first period in time, the biomarker in non-blood bodily fluid and measuring the same biomarker in the blood of the same individual to establish an R ratio of [NBBF1]/[BB1], where [NBBF1] is the biomarker concentration in non-blood bodily fluid in the first period in time, and [BB1] is the biomarker concentration in the blood in the first period in time; storing the ratio in a memory; measuring, in a second period in time, the biomarker in non-blood bodily fluid to determine [NBBF2], where [NBBF2] is the biomarker concentration in the non-blood bodily fluid in the second period in time; and correlating the measured [NBBF2] with the R ratio to generate a correlated [BB2] biomarker concentration in the blood of the individual in the second period in time.
Abstract: The present invention is related to correct the errors in instruments, operation, and others using intelligent monitoring structures and machine learning, and others.
Type:
Grant
Filed:
August 16, 2019
Date of Patent:
May 31, 2022
Assignee:
Essenlix Corporation
Inventors:
Stephen Chou, Wei Ding, Wu Chou, Jun Tian, Yuecheng Zhang, Mingquan Wu, Xing Li
Abstract: Among other things, the present invention provides devices and methods that stain a sample simply (e.g. one step) and quickly (e.g. <60 seconds), image it without wash, and generate, by a machine learning algorithm, a final image similar to a standard staining with wash.
Type:
Application
Filed:
June 2, 2020
Publication date:
May 12, 2022
Applicant:
Essenlix Corporation
Inventors:
Wu Chou, Hongbing Li, Susan Y. Sun, Xing Li, Wei Ding, Stephen Y. Chou
Abstract: Devices and systems are provided herein relating to a novel and rapid assay for tissue staining. Methods for using the devices and systems for analyzing tissue samples are also disclosed.
Abstract: Among other things, the disclosure of the present invention is related to make pathology and cytology faster, better and lower cost. The present invention also related to rapid intracellular assay.
Abstract: Disclosed is a method for correlating a biomarker in a non-blood bodily fluid with the same biomarker in the blood of an individual, including: measuring, in a first period in time, the biomarker in non-blood bodily fluid and measuring the same biomarker in the blood of the same individual to establish an R ratio of [NBBF1]/[BB1], where [NBBF1] is the biomarker concentration in non-blood bodily fluid in the first period in time, and [BB1] is the biomarker concentration in the blood in the first period in time; storing the ratio in a memory; measuring, in a second period in time, the biomarker in non-blood bodily fluid to determine [NBBF2], where [NBBF2] is the biomarker concentration in the non-blood bodily fluid in the second period in time; and correlating the measured [NBBF2] with the R ratio to generate a correlated [BB2] biomarker concentration in the blood of the individual in the second period in time.
Abstract: The present invention relates to the methods, devices, and systems that make bio/chemical sensing (including, not limited to, immunoassay, nucleic assay, electrolyte analysis, etc.) faster, more sensitive, less steps, easy to perform, smaller amount of samples required, less or reduced (or no) needs for professional assistance, and/or lower cost, than many current sensing methods and devices. The present invention also allow a test performed by a smartphone.
Type:
Grant
Filed:
February 7, 2018
Date of Patent:
March 15, 2022
Assignee:
Essenlix Corporation
Inventors:
Stephen Y. Chou, Wei Ding, Ji Qi, Yufan Zhang
Abstract: A device for sample analysis, including: a first plate, a second plate, spacers, a hinge, and an adhesive, wherein the first plate and the second plate are connected by the hinge and movable relative to each other around the axis of the hinge into different configurations, including an open configuration and a closed configuration as disclosed herein. Also disclosed are a kit, a system, and a method including the device.
Abstract: A device, including: an end-emitting optical fiber including a first end and a second end; a light extraction plate including a light scattering structure; and a light diffuser film disposed above the light scattering structure. Also disclosed are systems including the device.
Abstract: The present disclosure relates to devices, apparatus and methods of improving the accuracy of image-based assay, that uses imaging system having uncertainties or deviations (imperfection) compared with an ideal imaging system. One aspect of the present invention is to add the monitoring marks on the sample holder, with at least one of their geometric and/optical properties of the monitoring marks under predetermined and known, and taking images of the sample with the monitoring marks, and train a machine learning model using the images with the monitoring mark.
Type:
Application
Filed:
November 25, 2020
Publication date:
February 10, 2022
Applicant:
Essenlix Corporation
Inventors:
Xing Li, Wu CHOU, Stephen Y. CHOU, Wei DING
Abstract: A multi-mode illumination system, including: a first illumination module; a second illumination module; and a third illumination module, as disclosed herein.