Abstract: A confocal scanning microscope for scanning a sample has an illumination beam path that encompasses at least one point light source and a beam deflection device, and has further a detection beam path that encompasses at least one detection pinhole and the beam deflection device. The routing of the illumination beam path and/or of the detection beam path is adaptable to the scanning speed.

Abstract: A detection device, in particular for use in a laser scanning microscope, includes a means (2) located in a detection beam path (1) to spectrally split detection light into individual spectral components (3, 4), and further includes a deflection device (5) located downstream of the means (2) for spectral splitting to deflect the individual spectral components (3, 4) in different deflection directions onto detectors (6) assigned to the individual spectral components (3, 4). With a view to reliable separation of the individual spectral components (3, 4) deflected by deflection device (5), the detection device is built and further refined in such a way that at least one optical element (7) is arranged in the detection beam path (1) downstream of the means (2) for spectral splitting and upstream of the deflection device such that at least one spectral component (3, 4) of the light incident on the deflection device (5) is collimated in at least one spatial direction.

Abstract: An apparatus for optical scanning of multiple specimens (1) and/or specimen regions, the specimens (1) being associated with a specimen receiving device (2) and being optically scannable by a scanning device (3), is easy to operate for data recording of many specimens in the shortest possible time, using simple and economical optical system components. The apparatus according to the present invention is characterized in that the specimen receiving device (2) is rotatable about an axis (4).

Abstract: A microscope, in particular for confocal scanning microscopy, having a light source (1) for illuminating an object (6) to be investigated. An optical device (9, 12) is provided for splitting transmitted light (15) passing through the object (6) and fluorescent light (10, 13) produced in the object (6). A segmenting device (17) acting on the transmitted light (15) is configured with regard to a detection-light path which is as short as possible in such a way that the segmenting device (17) is arranged between the object (6) and the light-splitting device (9, 12).

Abstract: A method for measuring fluorescence correlations in the presence of slow signal fluctuations using a scanning microscope is disclosed. An illuminating light beam is positioned, with the scanning module, for a specific time period on a location of the specimen. Either a direct filtering is performed on the acquired data, or a Fourier analysis is performed. A suitable filter is then used to perform a filtering of the data subjected to the Fourier analysis. Lastly, a correlation function is calculated exclusively on the filtered data.

Abstract: An apparatus for determining directional transport processes with a scanning microscope (100) is disclosed. A deflection means (5) for coupling in an illuminating light beam (3), and a scanning module (7) for graphical display of a specimen (15) on a peripheral device (27) and for positioning the illuminating light beam (3) for a specific time period on a location of the specimen (15), are provided. Provided between the deflection means (5) and the scanning module (7) is a device (65) that generates, from the illuminating light beam (3), at least two illuminating light beams (3a, 3b) that merge at a rotation point (70) of the scanning module (7). Each of the several illuminating light beams (3a, 3b) generates a respective focus (72) in the specimen (15), all the foci (72) being arranged in one plane (75).

Abstract: A method for classifying a plurality of object image regions of an object to be detected using a scanning microscope includes labeling each of the image areas of the object with a different marker so that light of a respective characteristic emanates from each marker. The intensity of the light from each marker is detected using at least two channels so as to generate detection signals, each detection signal being a function of the intensity of the detected light. The object regions are classified using ratios of the detection signals.

Abstract: A microscope assemblage, in particular for confocal scanning microscopy, having a light source (1) for illuminating a specimen (6) to be examined and at least one fluorescent-light detector (11, 14) for the detection of fluorescent light (10, 13) generated in the specimen (6) and at least one transmitted-light detector (16) for the detection of transmitted light (15) passing through the specimen (6), is configured and developed, with a view toward reliable performing a wide variety of experiments with a high level of detection in each case, such that the fluorescent-light and transmitted-light detectors (11, 14; 16) are arranged in such a way as to make possible simultaneous detection of fluorescent and transmitting light (10, 13; 15).

Abstract: An apparatus for controlling optical power in a microscope includes a measuring device for measuring the optical power, and a control unit for controlling a high-frequency source as a function of the measured optical power so as to achieve a selectable level of the optical power. The microscope includes a source providing light along an illumination beam path to a sample, a detector receiving detection light lead along a detection beam path from the sample, and an acousto-optical or electro-optical element located in the illumination beam path and driven by the high-frequency source.

Abstract: An apparatus for ascertaining properties of a light beam, comprises a means for splitting a measured beam out from the light beam and comprises at least one detector that at least partially receives the measured beam. A polarization-influencing means is arranged in the beam path of the measured beam in order to enhance reliability and reproducibility.

Abstract: An EDP system having at least one EDP-assisted workstation (1, 2), in particular a PC (3, 4), computing system, process computer, network, etc., user-specific data that can encompass software adaptations or software settings, user interfaces, access authorizations, individually generated data, or the like being supplyable, is characterized in that the user-specific data are associated with a transportable, preferably miniaturized memory (5) that, for user-specific configuration of the workstation, communicates with the respective computer via a suitable interface (7, 8). A corresponding method serves for utilization of the system.

Abstract: A light source for illumination in scanning microscopy, and a scanning microscope contain an electromagnetic energy source that emits light of one wavelength, and a beam splitter for spatially dividing the light into at least two partial light beams. An intermediate element for wavelength modification is provided in at least one partial light beam.

Abstract: A device for adjusting the light beam in a microscope. The device couples the light beam into a housing of the device. A first and a second detectors are positioned at different distances to the coupling point. In the line of coupled in light beam at least one beam splitter is provided, which directs the coupled-in light beam onto at least one of the photo detectors.

Abstract: The present invention concerns an optical arrangement for selection and detection of the spectral region of a light beam (1) in a confocal scanning microscope, having a means (2) for spectral dispersion of the light beam (1), having means (3) for selecting a definable spectral region (4), and having a detection apparatus (5). The optical arrangement should be able to scan or detect multiple narrow-band spectral regions of a spectral region to be detected, in as uninterrupted a fashion as possible and in variably adjustable steps.

Abstract: A method for separating detection channels is disclosed, a sample (15) being equipped with at least two different fluorescent dyes. Firstly the emission spectrum of at least two fluorescent dyes is ascertained. From the emission spectra, the separation points of the wavelength and of the individual detection channels are determined. Lastly, adjustment of the separation of the at least two channels is accomplished on that basis.

Abstract: An apparatus for determining the light power level of a light beam (7), having a beam splitter (1) and a detector (11) associated with the beam splitter (1), is disclosed. The apparatus is characterized in that the beam splitter (1) splits measuring light (23) out of the light beam and conveys it to the detector (11), and that the ratio between the light power level of the light beam (7) and the light power level of the measuring light (23) measured at the detector (11) is constant over time.

Abstract: The invention discloses a fluorescence microscope comprising a light source that emits excitation light for illumination of a specimen, means for defining a two-dimensional search region for the excitation and detection wavelengths, means for selecting a subregion from the search region, at least one detector that detects detected light proceeding from the specimen, and a display for displaying an image of at least a portion of the specimen. Furthermore the invention discloses a method for fluorescence microscopy.

Abstract: The method and the system simplify moving interactions by means of virtual reference subjects and flux-based coordinate transformations in order to generate a changeable frame of reference.

Abstract: The invention relates to an optical arrangement provided for a spectral fanning out of a light beam (1), preferably the detection beam path of a confocal microscope, especially for the subsequent splitting of the fanned out beam (2) out of the dispersion plane thereof. The optical arrangment is also provided for detecting the fanned out spectral regions (4), whereby the incoming light beam (1) is focused on a pinhole (7). The invention is characterized in that the pinhole (7) has a polygonal passageway (8) in order to realize a high dynamic response when the light beam is split into spectral regions (4) or into spectral colors.

Abstract: A scanning microscope has a light source that emits illuminating light for illumination of a specimen, at least one first detector for detection of the detected light proceeding from the specimen, an objective arranged in both an illumination beam path and a detection beam path, and a coupling-out element that is selectably for descan detection and non-descan detection positionable in the illumination and detection beam path. A light-guiding fiber is provided for transporting at least a portion of the detection light from the coupling-out element to the first detector.