Abstract: A lab-on-a-chip (LOC) for the biomimetic study of the multicellular interactions of bone cells includes a PDMS substrate and cap, which together form one or more wells that are fluidly coupled by tubes. The wells are configured to support various bone cells and related cellular support substrates therein, while the tubes allow conditioned medium (CM), including soluble signals, and various other co-factors to be communicated among the various wells. By controlling the configuration among and between various bone cells in the wells, the temporal and spatial limitations associated with traditional in vivo bone tissue models is removed. In addition, the LOC enables a particular research objective to be studied by allowing the user to configure the arrangement of the wells/tubes of the LOC, so as to control the manner in which bone cell soluble signals, bone cell contact, and bone cell matrix interaction interplay.

Abstract: Provided is an assembled bioreactor chamber comprising an upper end portion, at least one intermediate portion and a lower end portion for forming a chamber of the reactor chamber. The upper end portion and the lower end portion are respectively provided with an interface connected with a culture medium line, and the upper end portion and the intermediate portion, and the intermediate portion and the lower end portion are detachably connected in a sealed manner through a connecting mechanism; when the number of the intermediate portions is ?2, the adjacent intermediate portions are detachably connected in a sealed manner through a connecting mechanism; and the chamber also comprises a cell carrier for cell attachment growth.

Abstract: Provided is a highly reliable temperature adjustment apparatus which uses a temperature adjustment element and quantitatively evaluates the temperature adjustment performance of the temperature adjustment element. The temperature adjustment apparatus is provided with: a temperature adjustment element; one or more temperature detection elements provided near the temperature adjustment element; a calculation unit for calculating the output of the temperature detection element; and a display unit for displaying at least one of a time calculated by the calculation unit as a time at which the temperature adjustment performance of the temperature adjustment is predicted to be below a desired level, the number of operations and the time of current conduction or a warning based on the result of calculation.

Abstract: A method for carbon resource utilization is disclosed. According to one embodiment, the method includes (a) supplying carbon dioxide to a medium to form bicarbonate ions (HCO3?) (S100), (b) inoculating one or more microalgal species into the medium, followed by photo-culture (S200), and (c) supplying calcium ions (Ca2+) to the medium, where the microalgal species are photo-cultured, to produce calcite (CaCO3)-containing biomass (S300).

Abstract: A cell culture container includes a storage container, a cell culture area, a culture solution supply unit, and a discharge unit. The cell culture area is a region for culturing cells, and is provided in the storage container. The culture solution supply unit supplies the culture solution into the cell culture area at a flow rate equal to or less than a predetermined flow rate. The discharge unit discharges the liquid in the cell culture area to the outside of the storage container. A rectification unit is a structure in which a plurality of outflow ports are uniformly arranged in a direction perpendicular to the flow direction of the culture solution from the culture solution supply unit to the discharge unit.

Abstract: The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container having an accommodating space (2) for accommodating the sample and comprising an indicating apparatus, which can be arranged on the outside of the sample container, for monitoring at least one temperature limit value. The indicating apparatus has at least one cavity, which is only partially filled with an indicating substance, the melting temperature of which lies in a range from ?20 ° C. to ?140 ° C. The indicating apparatus can be designed, in particular, as a cylindrical body, which can be fastened to a cryotube as a bottom part, or alternatively as a double-walled hollow cylinder, which can be slid onto an outer lateral surface of the cryotube. The indicating apparatus can also be fastened to a lateral outer wall of the sample container, e.g. as a hollow body that can be inserted into a sleeve or insertion pocket.

Abstract: In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.

Abstract: An ultraviolet light sterilization device for treatment of plant materials, having a rotational treatment chamber having an axis of rotation and at least one mechanical agitation element, an ultraviolet light device that is positioned inside the rotational treatment chamber and is further positioned generally parallel to and congruent with the axis of rotation and where the ultraviolet light device and the rotational treatment chamber are connected to one another via a rotational bearing such that the rotational treatment chamber may be rotated about the axis of rotation while the ultraviolet light device does not rotate, and where the ultraviolet light device is electrically coupled to a power source via an electrical cable that passes through an aperture in the rotational bearing.

Abstract: A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

Abstract: The present invention relates to a novel microfluidic device and a microfluidic system for culturing cell samples based on: one or more culture wells (2) forming cell housing volumes for three-dimensional cell culture; one or more capillary channels (1) for circulating cells and/or fluid media, said channels (1) being connected to the wells (2) as well perfusion means; and an inlet port (3) and an outlet port (3?) for seeding cells and/or for circulating fluid in said channel (1), said ports (3, 3?) being connected to the ends of each capillary channel (1). The novel configuration of the ports (3, 3?) and their corresponding channels (1) allows known microfluidic perfusion media to be improved, dispensing with the use of complex elements for injecting the fluid media, such as syringe pumps, for example. The invention also allows two-dimensional and three-dimensional culture studies to be performed simultaneously.

Abstract: The present invention relates to a disinfecting composition which requires a shorter contact time to be effective than similar compositions. Also, provided is a method of disinfecting a surface with the disinfecting composition where a shorter contact time is needed to disinfect the surface.

Abstract: A flask assembly that includes: an Erlenmeyer flask; and a liner bag comprising a single opening the bag sized to fit within the flask and having a thickness from about 0.0254 mm to about 0.508 mm. The liner bag is configured for cell culturing and ease of insertion of the bag into the flask. In addition, the liner bag comprises a gas-permeable, polymeric material. Further, the liner bag can include a pocket adapted to receive a removable flask insertion element and/or at least one foldable seam configured to facilitate insertion of the bag into the flask. The liner bag can also include a vent having a gas permeability that is greater than the gas permeability of the gas-permeable, polymeric material employed in the balance of the bag.

Abstract: An apparatus for perfusing an organ or tissue including a perfusion circuit for perfusing the organ or tissue with liquid perfusate; and an oxygenation system for oxygenating perfusate that recirculates through the perfusion circuit. The oxygenation system includes an oxygen circuit for delivering oxygen to the liquid perfusate and an air circuit for delivering ambient air to the liquid perfusate. The air may be pumped through the oxygenation system via an air pump. The air or oxygen may be bubble through the perfusate to increase oxygen levels in the perfusate. Such supplemental oxygen may beneficial during hypothermic preservation of organs.

Abstract: The present invention relates to a novel process for producing hemicellulose fractions from cereal crops, comprising pressurized hot water extraction of hemicellulose fractions from cereal crops in combination with subsequent enzymatic treatments and/or subsequent membrane ultrafiltration to further purify the extracts. The novel process described herein allows for sustainable and scalable extraction and isolation of valuable hemicellulose fractions, especially arabinoxylans (AXs), from cereal side products. The process allows the selective fractionation of arabinoxylans based on their molecular structure and inherent functionalities, which can e.g. be used for the preparation of carbohydrate-based materials with functional properties.

Abstract: A disinfectant device including a pouch, the pouch including a first layer of material coupled to a second layer of material and defining a compartment therebetween. At least one dry reactant is retained within the compartment, the at least one dry reactant producing chlorine dioxide gas when exposed to water. A wick is at least partially disposed within the compartment. At least one from the group consisting of the first layer of material and the second layer of material define an array of ports along a periphery of the pouch and an array of fluid conduits, the array of fluid conduits being proximal to the array of ports, offset from the array of ports, in fluid communication with the wick, the compartment, and the array of ports.

Abstract: A system for receiving a disposable bag (44) has a receiving container (10) with a container interior for receiving the disposable bag (44) and a temperature-control hollow wall (20) that at least partially surrounds the container interior of the receiving container (10). A temperature-control unit controls the temperature of the container interior by will a temperature-control medium arranged in the temperature-control hollow wall (20) at a maximum pressure of about 1 bar.

Abstract: A device for cell culture comprising: a main body (11); said main body (11) comprises a plurality of circular portions (21-23); said device comprises a plurality of caps (51-53); each of said plurality of caps (51-53) comprises a base structure (58) having a circular hole (57), housing an upper slide (54) and an elastomeric layer (56) secured to said slide (54); said elastomeric layer (56) has a rectangular hole (59); said plurality of caps (51-53) being adapted to cooperate with said plurality of circular portions (21-23); each of said plurality of circular portions (21-23) each comprise an inlet hole (64-66) and an outlet hole (67-69) aligned with the long side of said rectangular hole (59), to perfuse the culture chamber (12-14) located in said rectangular hole (59) of said elastomeric layer (56).

Abstract: Vessel closure assemblies are provided. The vessel closure assemblies may be engaged with vessels. The vessel closure assemblies may each include a vessel closure, inserts such as anchors and fluid conduits extending through the vessel closure, and a respiratory assembly. The respiratory assembly may include a housing and one or more gas permeable membranes. The gas permeable membranes may be oriented substantially perpendicular to a top wall of the vessel closure through which the inserts extend.

Abstract: A method and system of and for handling, preserving, separating, filtering, collecting, manipulating, and/or culturing ex vivo biological material including red blood cells, white blood cells, and blood plasma within a bioreactor having a gas permeable membrane that allows for passive ventilation.

Abstract: Systems and methods for providing ultraviolet (UV) sterilization, disinfection and decontamination of electronic gaming machines (EGMs), gaming chips, dice, playing cards, currency, TITO tickets, etc. The ultraviolet sterilization, disinfection and decontamination may include a singular or plurality of UV lamps of any type or style or UV LEDs or RGB-UV LEDs or any type or style of UV LEDs of at least such wavelengths to be effective in at least partially reducing or eliminating viruses or the like. The UV sources are mounted on, in or proximate to a variety of gaming devices or equipment such as EGMs, mechanical gaming machines, chip trays, chippers, dice holders, automatic card shufflers, bill validators, currency counting devices, currency dispensing devices, printers, magnetic card readers, playing card and currency sterilization, disinfection or decontamination storage mechanisms, ATMs, redemption machines, promotional kiosks, etc., or similar devices used in other industries.