Abstract: A scaffold-stretching system includes at least one stretchable loading chamber configured to support a scaffold material and a supply of cells, such as human skin substitute cells, and is configured to allow for cultivation of a cellular three-dimensional scaffold; and a mechanical loading system is configured for application of cyclic and static uniaxial tensile mechanical loading on the cellular three-dimensional scaffold, and is configured to mimic the in vivo environment of musculoskeletal, cardiovascular, and other tissues that experience uniaxial strains.
Abstract: The present invention provides various improved systems and methods for obtaining, generating, culturing, and handling cells, such as stem cells (including induced pluripotent stem cells or iPSCs) and differentiated cells, as well as cells and cell panels produced using such systems and methods, and uses of such cells and cell panels.
Type:
Grant
Filed:
September 30, 2019
Date of Patent:
October 5, 2021
Assignee:
New York Stem Cell Foundation, Inc.
Inventors:
Scott Noggle, Kevin Eggan, Stephen Chang, Susan Solomon
Abstract: An electroporation apparatus comprising an elongated hollow member in order to provide a uniform electric field during electroporation, wherein specifically, electroporation is carried out by applying electric pulses through a couple of electrodes from both end parts of the elongated hollow member, after the hollow member is charged with fluid specimen including cells and material which would be injected into the cells.
Abstract: The present invention provides for methods and at least partially automated devices suitable for producing a transplantable cellular suspension of living tissue suitable for promoting tissue regeneration in an epithelium-related procedure, as well as compositions produced therefrom. Tissue regeneration in humans is extremely limited and constitutes a major challenge to the repair of damaged organ function. Wound treatment is a typical area where tissue regeneration is required. Wounds (lacerations or openings) in mammalian tissue can result in tissue disruption and coagulation of the microvasculature at the wound face.
Type:
Grant
Filed:
February 11, 2020
Date of Patent:
September 21, 2021
Assignee:
AVITA MEDICAL LTD
Inventors:
Andrew Perry Quick, David Allen Fencil, William Ford Dolphin
Abstract: A cell culture monitoring system includes measuring units housed in an incubator and each equipped with an indicator, the measuring units each measuring a state of cells in a culture container mounted therein; and an administration unit. The administration unit includes a receiver that receives, from each of the measuring units, a state of the cells measured, the state being associated with identification information of the measuring unit; an analyzer that analyzes the state of the cells and computes information indicating a culture state; a storage that stores, in association with each other, the information indicating the culture state and the identification information; a display that displays, in association with each other, the information indicating the culture state and an identification based on the identification information; and a controller that controls the indicators so that each of the indicators displays the identification corresponding to the identification displayed on the display.
Type:
Grant
Filed:
May 23, 2018
Date of Patent:
September 21, 2021
Assignee:
OLYMPUS CORPORATION
Inventors:
Naohiro Ariga, Toshiyuki Hattori, Mina Kobayashi, Ayumu Sakurai
Abstract: A sealable container for preservation of a biological sample is provided. The container includes a cap comprising a proximal open and a lumen and an elongate body that extends from a handle portion to a specimen portion. A closure portion is disposed between the handle portion and the specimen portion, wherein the closure portion is configured to make surface to surface contact with the cap when the specimen portion is fully inserted within the lumen. The closure portion comprises an outer diameter that gradually decreases in a direction from the handle portion to the specimen portion. The specimen portion comprises a scooped portion where a distance between the upper surface and the lower surface is less than distances between the upper surface and the lower surface along remaining portions of the specimen portion, wherein the scooped portion is spaced proximally from a distal tip of the specimen portion.
Type:
Grant
Filed:
September 26, 2019
Date of Patent:
September 14, 2021
Assignee:
Cook Medical Technologies LLC
Inventors:
Jorge L. Jimenez-Rios, Lyle D. Hundley, Victor W. Havill, Erin Elizabeth Roberts, Jaimie Michelle Jarboe
Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
Type:
Grant
Filed:
April 14, 2021
Date of Patent:
September 14, 2021
Assignee:
Inscripta, Inc.
Inventors:
Nathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
Abstract: A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position.
Abstract: Generation of energy and storage of energy for subsequent use is provided by electromethanogenesis of carbon dioxide into a fuel gas and the storage of the fuel gas for subsequent use. An electromethanogenic reactor includes an anode conductor and a cathode conductor wherein the cathode conductor includes submicron to micron scale pores. Electromethanogenesis microbes and/or enzymes are located in the micron scale pores of the cathode electrode conductor. Carbon dioxide is introduced into the electromethanogenic reactor and the electromethanogenesis microbes/enzymes and the carbon dioxide interact and produce a fuel gas. The fuel gas is stored for subsequent use, for example use in power generation.
Type:
Grant
Filed:
April 10, 2018
Date of Patent:
September 7, 2021
Assignee:
Lawrence Livermore National Laboratory, LLC
Inventors:
Jennifer Marie Knipe, Sarah E. Baker, Marcus A. Worsley, Swetha Chandrasekaran
Abstract: A sample-reagent mixture is thermal cycled through a plurality of cycles. Each thermal cycle includes actuating a heater to heat the sample-reagent mixtures; and dispensing a fluid onto the sample-reagent mixture to cool the sample reagent mixture.
Type:
Grant
Filed:
January 29, 2016
Date of Patent:
September 7, 2021
Assignee:
Hewlett-Packard Development Company, L.P.
Inventors:
Bradley D. Chung, Alexander N. Govyadinov
Abstract: The present invention provides a culture vessel with which cells can be aseptically cultured and aseptically transferred to a place where the cells are to be used (such as an operating room) and from which the cells can be simply taken out. The present invention provides a closed culture vessel including an openable airtight vessel, and a cell culture member disposed in the airtight vessel and including a cell culture surface, in which the cell culture member is provided detachably from the airtight vessel.
Abstract: Embodiments described herein relate to apparatus for temperature modulation of samples. Generally, aspects of the disclosure provide for a portable cooling and heating apparatus which enables a user to visualize a sample during cooling or heating. In one embodiment, the apparatus include a sample block, a plurality of thermoelectric modules coupled to the sample block, and a cooling block coupled to the plurality of thermoelectric modules. In another embodiment, the cooling block is in fluid communication with a fluid reservoir. Other embodiments utilize various insulating materials to influence thermal conductivity between the sample block and the cooling block to provide for enhanced temperature control and modulation.
Abstract: Disclosed herein are implantable 3-dimensional large capacity device assemblies, specifically, large capacity device assemblies for encapsulating pancreatic progenitor cells for treatment of diabetes.
Type:
Grant
Filed:
November 11, 2016
Date of Patent:
August 3, 2021
Assignee:
ViaCyte, Inc.
Inventors:
Vincent So, Laura Martinson, Chad Green, Michael Scott, Mario Zamarripa
Abstract: Reactor for enzymatic hydrolysis of a raw material comprising in sequence: i)—a first heat exchanger adapted to heat the raw material supplied to the reactor to a temperature within a range that favours enzymatic hydrolysis, ii)—a reactor comprising plural in reactor chambers connected in series, separated by closable valves, iii)—a second heat exchanger adapted to heat the reaction mixture to a temperature higher than the temperature range favouring enzymatic hydrolysis, the reactor being formed with inclined tubular reactor chambers assembled to form a reactor with vertical axis, the first reactor chamber being the vertically uppermost chamber of the reactor, while at least one reactor chamber is adapted to be stirred with a through-flowing inert gas.
Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
Type:
Grant
Filed:
February 13, 2021
Date of Patent:
July 27, 2021
Assignee:
Inscripta, Inc.
Inventors:
Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.
Type:
Grant
Filed:
June 10, 2020
Date of Patent:
July 27, 2021
Assignee:
The University of British Columbia
Inventors:
Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
Abstract: Digester (1) having an agitating device (10) and an agitating device (10) and a method for manufacturing an agitating device (10), the agitating device (10) comprising multiple agitator blades (21-29) which agitator blades (11-13) comprise a plurality of blade sections (21-29) angled relative to one another. The blade sections (21-29) of the agitator blade (11-13) are angled relative to one another such that the agitator blade gradient (11-13) decreases with the distance (30) from a central rotational axis (19) increasing to configure the agitator blade in a flow-optimized three-dimensional shape.
Abstract: A device for treating organic materials having a nozzle base, a roof, a rear wall, and two side walls, delimiting the interior of the device. Extending in the nozzle base are tubes connected to nozzles in the nozzle base, directed toward the interior of the device. The tubes are connected to a process gas apparatus for supplying the tubes with process gas. The process gas apparatus includes a ventilator connected to a pressure chamber for feeding the pressure chamber with process gas. The pressure chamber has at least one outlet connected to at least one of the tubes in the nozzle base to conduct process gas from the pressure chamber into the tubes. The ventilator and the pressure chamber are situated on the roof of the device, in particular in an enclosure. The pressure chamber is connected to the tubes via at least one connecting line in the rear wall.
Abstract: The invention relates to a device for hydrating with a liquid sample, the device comprising a container (4) for receiving at least one liquid sample, and a lid (5) possessing a bottom face (51) having at least one hydrating support (3) fastened thereto to present an absorption face (31) for absorbing a liquid sample. According to the invention, the container (4) presents, by means of at least one well (6), a calibrated volume for receiving a liquid sample, the at least said well presenting a hydrating calibrated open section for hydrating a hydrating support (3) defined by the top edge (8) of at least said well (6), the hydrating calibrated open section possessing an area that is less than the area of the absorption face of the hydrating support (3) in order to control the absorption by capillarity of the liquid sample by the hydrating support.
Type:
Grant
Filed:
June 6, 2016
Date of Patent:
July 13, 2021
Assignee:
BIOMERIEUX
Inventors:
Bruno Colin, Laurence Dévigne, Sandrine Ghirardi, Marie-Pierre Montet, Christine Rozand, Philippe Wandels
Abstract: A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.