Abstract: The present disclosure relates to a tempering block module for the thermal treatment of samples, comprising: a tempering block; and an ejection mechanism for lifting reaction vessels off the tempering block, the ejection mechanism including first and second ejection plungers that are movably mounted in the tempering block module perpendicular to the tempering block from a first position, retracted into the tempering block module, into a second position, extended out of the tempering block module, and wherein the tempering block module includes a first plunger drive connected to the first ejection plunger for driving the movement of the first ejection plunger and a second plunger drive, different from the first plunger drive, connected to the second ejection plunger for driving the movement of the second ejection plunger from the first to the second position or from the second to the first position.

Abstract: A cell culture device includes a main body and a plug element. The main body includes a slot, an open groove and a fluid chamber. The open groove is connected with one side of the slot. The fluid chamber is disposed inside the main body and connected with another side of the slot. The plug element includes a first cell culture chamber and a first porous membrane. The first porous membrane is disposed at one side of the first cell culture chamber. The plug element is detachably plugged into the slot. When the plug element is plugged into the slot, the first cell culture chamber is communicated with the open groove to form an open space, and the open space and the fluid chamber are separated by the first porous membrane.

Abstract: A collection pipette that collects a microscopic object includes a first tube part, a second tube part connected to an end of the first tube part, and a third tube part connected to the other end of the first tube part. The longitudinal direction of the third tube part intersects with the longitudinal direction of the first tube part, and is parallel to the longitudinal direction of the second tube part. For example, the length in the longitudinal direction of the third tube part is shorter than the length in the longitudinal direction of the first tube part.

Abstract: The present invention relates to a device (10) for obtaining a sample (30) from a biological material (40) in solid form, said device comprising an array of micro-needles (30) arranged on a base plate (20). It further relates to a method for obtaining a sample (50) from a biological material (40) in solid form, comprising pressing the micro-needles (30) of the device (10) into said biological material (40), and subsequently removing the device from the biological material (40), and to the use of the device (10) in such a method.

Abstract: Systems and methods provide a self-contained sealed apparatus that captures, filters, compresses and stores methane gas produced by the decomposition of bio-degradable organic materials. The system includes a rotatable and sealable chamber with an intermittent drive unit that mixes moist bio-degradable material during an anaerobic reaction, and captures methane gas generated by anaerobic decomposition. A filter to remove impurities, a low-pressure storage tank, a compressor and a high-pressure storage tank are interconnected and controlled by a system that monitors system parameters, that may include gas flow rate, temperature, and gas volume, and controls system parameters, that may include drive unit activation, generator operation, and compressor operation.

Abstract: Described herein are microfluidic devices and systems for high density cell culture and/or high throughput cell assays. Methods of using the same are also provided herein. In some embodiments, the microfluidic devices and systems described herein provide rapid and automated trapping of single embryos in ordered arrays.

Abstract: A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

Abstract: An incubator (110, 140) for the reception of an individual sample carrier, a shelving system (100) for such incubators, a transport container for such incubators, as well as a modular system are suggested, wherein the incubators can be inserted and withdrawn (130) independently of one another, can be supplied with electricity, water and a gas via corresponding connections (150), and can communicate with a computer network via a wireless connection. Such a system reduces the risk of contamination or confusion in the lab and avoids interruptions in the monitoring and controlling of the environmental conditions in the respective incubators. These can rather independently maintain the most favourable environmental conditions for the respective samples, both during storage and also during a transport operation. The insertion and withdrawal of individual incubators from the shelving system does not, or only very briefly, interrupt the monitoring and control of the conditions in the interior of the incubators.

Abstract: A device with integrated methods for carrying out reverse transcription polymerase chain reaction (RT-PCR) and/or array based analysis involving signal generating agents (gold nanoclusters) comprising a heating and cooling cycle based synthesis selectively with DIMA and protein as templates. The advancement is further directed to a portable device adapted for RT-PCR and array based gene and protein expression analyses based on a common detection agent involving luminescence of in-situ synthesized gold nanoclusters with adaptability for user friendly graphical user interface (GUI) for controlling, visualization and analysis of the data.

Abstract: A thermal block assembly is provided. The assembly can comprise a substrate comprising a first surface configured with a plurality of reaction sites each reaction site configured to contain a biological sample and a sample block comprising a plurality of pedestals configured to thermally modulate the plurality of biological samples wherein each pedestal is thermally coupled to one of the reaction sites. The assembly can further comprise cooling blocks, slots and insulating rings associated with reaction sites each capable of minimizing heat flow between reaction sites. A method for thermally isolating reaction sites is also provided.

Abstract: The present disclosure relates to a device and method for remedying a heavy-metal-polluted soil with a microorganism, and relates to a device for remedying a metal-polluted soil and a remediation method thereof. The present disclosure solves the problem that a remediation microorganism cannot be extracted from the soil. The device comprises a main plate and a plurality of microorganism-extracting chambers, wherein the main plate is connected to the microorganism-extracting chambers; the microorganism-extracting chamber are filled with a filler; a tail end of the microorganism-extracting chambers is spiky, and openings are separately provided on a side wall of the microorganism-extracting chambers. The method comprises: I, adding a nutrient medium to the soil, and then inoculating a remediation microorganism; and II, inserting the above device into the soil for 6˜8 days, and then taking it out. The method can remedy heavy-metal-polluted soil in a short time with a high removal rate of the heavy metal.

Abstract: The present invention provides a method for culturing cells in a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the container is composed of a flexible material, comprising culturing cells in a culture medium in the cell culture container.

Abstract: Methods of decellularization of tissue, such as mammalian tissue, are provided, along with methods of making an extracellular matrix (ECM) preparation. Systems and apparatus useful in performing the methods are also provided.

Abstract: The devices, methods, and compositions disclosed herein accomplish robust cell encapsulation in polymer microparticles using a vertically oriented microfluidic device. A hydrophilic polymer precursor solution is flowed into a first inlet channel, which extends inward from an upper surface of the device housing. A hydrophobic fluid is flowed into a second inlet channel, which extends inward from a lower surface of the device housing. The two inlet channels meet at a junction, and an outlet channel extends away from the two inlet channels. When the two inwardly flowing streams meet at the junction, the polymer precursor solution disperses into the hydrophobic fluid. The dispersed precursor droplets are photopolymerized into microparticles as they travel through the outlet channel. The resulting microparticles are highly uniform, and are larger than conventionally formed microparticles. Cells of varying types can be encapsulated with high viability and spatial uniformity.

Abstract: Provided is a culture bag accommodating a culture fluid, the culture bag is capable of suppressing foaming in the culture fluid when oscillating the culture fluid to perform a culture, and performing a culture with high efficiency. The culture bag includes a culture space accommodating a culture fluid, the culture space being an endless space to allow the culture fluid to circulate therein, in which the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a first surface formed of a fine structure and a second surface formed of a structure different from that of the first structure.

Abstract: A bioreactor system for conditioning of pluripotent cells or cell media is provided. In further aspects, conditioned pluripotent cells and methods for making such cells are provided.

Abstract: A washing treatment system includes an odor and flue gas washing tower, a biological deodorization filtering tower, a multifunctional biomass combustion boiler, a liquid fermentation reactor, a solid fermentation reactor, circulating pumps, an exhaust fan and an induced draft fan. An exhaust port is formed in a top end cover of the odor and flue gas washing tower. A liquid inlet, an air inlet and a liquid drainage port are formed in a side wall of a tank body. A hanging basket is placed in the tank body. Organic fillers and/or inorganic fillers are placed in the hanging basket. An inner cavity of the washing tower is divided into a liquid inlet shunting cavity, a filler layer, an air cavity and a liquid accumulation cavity from top to bottom. An upper supernatant in the liquid fermentation reactor is connected with the liquid inlet for washing.

Abstract: Disclosed are an apparatus and methods for rapid amplification of nucleic acids. More particularly, the present disclosure relates to an apparatus for mixing a reaction solution during amplification of nucleic acids and to methods for amplifying nucleic acids. Also disclosed are methods for lysing cells in a sample and amplifying nucleic acids.

Abstract: The present invention provides a cell culture vessel or a sample cell for observation use that makes it possible to observe three-dimensionally-cultured cells from various angles. The cell culture vessel or the sample cell for observation use according to the present invention is characterized by being equipped with a culture gel in which a cell or cell tissue is embedded and a first vessel which encloses the culture gel, wherein a space in the first vessel is filled with the culture gel; and the first vessel has a light-permeable window made from a hydrogel.

Abstract: A device and method for preserving corneal graft tissue. In one embodiment, a device for preserving corneal graft tissue comprises: a first chamber; a second chamber; and a corneal graft tissue suspension assembly that is configured to retain and suspend the corneal graft tissue between the first chamber and the second chamber, the first chamber being fluidly isolated from the second chamber when the corneal graft tissue is graft tissue is retained and suspended within the corneal graft tissue suspension assembly. In one embodiment, a method includes filling a first chamber of a device with a first preservation medium and filling a second chamber of the device with a second preservation medium different than or the same as the first, a corneal graft tissue being located between the first and second chambers.