Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of LI and L2.

Abstract: Provided herein, in one aspect, is an improved nanotrain for use in connection with a nanopore device for single-molecule detection. Methods for making and using the same are also provided.

Abstract: The present disclosure relates to a device, system and method for sensing functional motions of a single protein molecule via direct attachment of one or more electrodes to the molecule. The present disclosure also relates to an array, a system comprising an array and method for sequencing a biopolymer using an array.

Abstract: This disclosure provides, in one aspect, a platform and related methods to detect diagnostic markers or biomarkers for various diseases. In some embodiments, the platform can be a lateral flow test strip having a hydrogel composition deposited on one or more test zones.

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of LI and L2.

Abstract: The present invention is related to engineered nucleic acid bases for use in molecular electronics, such as nanosensors, molecular-scale transistors, FET devices, molecular motors, logic and memory devices, and nanogap electronic measuring devices for the identification and/or sequencing of biopolymers.

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of LI and L2.

Abstract: The present invention provides methods to engineer enzymes for their integration into a molecular nanowire as a fum-tional component for biopolymer sequencing/identification. The enzymes include but are not limited to DNA polymerase, RNA poly-merase, DNA helicase, DNA ligase, DNA exonuclease, reverse transcriptase, RNA primase, ribosome, sucrase, or lactase, which are either natural, mutated, or synthesized.

Abstract: The present invention relates to methods for creating conductive nanojunctions using metalized or conductive polymer joined to a DNA nanowire in a nanodevice for chemosensing and biosensing.

Abstract: The present invention relates to a nanopore device with a motion control mechanism to control the speed of a polymeric molecule translocating through the nanopore for a tunneling nanogap to read out its sequences or components.

Abstract: This invention provides methods to identify or sequence a DNA or RNA molecule electronically in a single molecule level based on polymerase synthesis.

Abstract: This invention provides a device for sequencing and identification of biopolymers electronically.

Abstract: This invention provides a nanostructure device and method for the sequencing or identification of biomolecules based on in vitro template-directed enzymatic replication or synthesis.

Abstract: The present disclosure provides a method for identifying and quantifying sulfated glycosaminoglycans, including for example heparin, by passing a sample through nanopores. The glycosaminoglycans sample is measured in microliter quantities, at nanomolar concentrations with detection of impurities below 0.5%, and a dynamic range over five decades of magnitude with a trained machine learning algorithm.

Abstract: This invention is related to electronic identification and sensing of biomolecules using enzymes incorporated into nanostructures constructed with conductive peptides and/or peptide complexes.

Abstract: This invention provides methods to construct a system for the sequencing of biomolecules based on in vitro template-directed enzymatic replication or synthesis. Embodiments of the present invention are related to systems, methods, devices, and compositions of matter for the sequencing or identification of biopolymers using electronic signals. More specifically, the present disclosure includes embodiments which teach the construction of a system to detect biopolymers electronically based on enzymatic activities, including replication.

Abstract: This invention provides a system and related devices, methods, and molecular modules for electronically sequencing biopolymers.

Abstract: The present disclosure provides apparatus and methods for determining the sequence of a nucleic acid. The apparatus comprises electrodes that form a tunnel gap through which the nucleic acid can pass. The electrodes comprise a reagent that is capable of selectively interacting with a nucleobase of the nucleic acid sequence. When the reagent interacts with a nucleobase, a detectable signal is produced and used to identify the nucleobase of the nucleic acid. Advantageously, the apparatus of this disclosure is specific to identifying nucleic acids.

Abstract: The present disclosure provides an apparatus for synthesizing a biopolymer, a method for preparing an apparatus for synthesizing a biopolymer, and a method of synthesizing a biopolymer. The apparatus comprises (a) a substrate comprising a top surface and a plurality of wells, wherein each of the plurality of wells comprises a first electrode disposed on the bottom of the well and a linker attached to the sides of the well; and (b) a fluidic chamber system disposed on the top surface of the substrate.

Abstract: The present disclosure provides apparatus and methods for determining the sequence of a nucleic acid. The apparatus comprises electrodes that form a tunnel gap through which the nucleic acid can pass. The electrodes comprise a reagent that is capable of selectively interacting with a nucleobase of the nucleic acid sequence. When the reagent interacts with a nucleobase, a detectable signal is produced and used to identify the nucleobase of the nucleic acid. Advantageously, the apparatus of this disclosure is specific to identifying nucleic acids.