Abstract: Disease components can be detected in a fluid using magnetic particles and microfluidics. Magnetic particles are combined with a sample in a fluid. The sample may include disease components. The magnetic particles can be configured to tag any disease components within the sample. The fluid can be forced into a microchamber with two microcompartments. In the first microcompartment, the fluid can be exposed to a magnetic field and/or magnetic field gradient. The tagged disease component can be trapped by the magnetic field and/or magnetic field gradient due to the magnetic particles, allowing nonmagnetic components of the fluid to be washed away while the tagged disease components remain trapped by the magnetic field and/or magnetic field gradient. Then, the disease components can be forced into a more narrow second microcompartment and detected using optical instruments.

Abstract: This disclosure relates to modified guide RNAs having improved in vitro and in vivo activity in gene editing methods.

Abstract: A system is described herein that can be used to perform magneto-optical detection of a disease component in a test sample using magnetic nanoparticles. A concentration of magnetic nanoparticles and a concentration of bindable agents can be administered to the test sample. The magnetic nanoparticles can be configured to attach to the bindable agents. A light beam can be transmitted through the test sample to a light detector. A magnetic field gradient can be established through the test sample. If the transmitted light beam under the magnetic field gradient exhibits a variable intensity change during a time period, the disease component can be determined to exist in the test sample.

Abstract: The present invention is directed to methods of probiotic selection and use based on the capability of microbial biogenic amine uptake as a method for targeted clinical and veterinary applications, including promoting health and well-being and/or treating therapeutic conditions. The present invention utilizes a microbially-focused approach for the development of drug selection and/or probiotic administration in a variety of diseases and disorders.

Abstract: A fluid processing system includes a disposable fluid circuit and reusable hardware configured to accept the disposable fluid circuit. The disposable fluid circuit includes a spinning membrane separator, first and second syringes, and a flow control cassette. The reusable hardware includes a spinning membrane separator drive coupled to the spinning membrane separator, first and second syringe pumps coupled to the first and second syringes respectively, a control cassette interface coupled to the flow control cassette, and at least one controller coupled to the spinning membrane separator drive, the first and second syringe pumps, and the control cassette interface. The controller is configured to selectively operate the drive, the first and second syringe pumps, and the interface to provide a procedure according to a protocol.

Abstract: Embodiments of the disclosure provide a thermal model based on an adaptive filter bank for characterizing heat transfer of a volume of a thermal system. In one embodiment, the adaptive filter bank is used for diagnostics that provides information related to the condition of a thermal system. The diagnostics are based on an analysis of heat transfer characteristics of a dynamic representation of the thermal system. In accordance with the embodiments, thermal coefficients are generated based on an adaptive filter bank. One or more filters are applied to the thermal coefficients based on a sampling rate and one or more estimate thermal coefficient thresholds are generated based on the sampling rate. It is determined whether at least one of the thermal coefficients that is filtered satisfies at least one of the estimated thermal coefficient thresholds. Thereupon, alert information indicative of a diagnostic event is provided based on the determination.

Abstract: A frame error correction circuit may identify and correct errors in data frames provided to a receiver as part of a diversity communications scheme. The frame error correction circuit may further align the data frames so that the data frames can be compared. The frame error correction circuit may perform a bit-wise comparison of the data frames and identify inconsistent bit positions where bits in the data frames differ from one another. Once inconsistent bit positions have been identified, the frame error correction circuit may access a permutation table of permutations of bits at the inconsistent bit positions. In some implementations, the frame error correction circuit uses the permutation table to reassemble permutations of the data frames. In various implementations, the frame error correction circuit performs a CRC of each permutation of the data frames, and provides a valid permutation to a network.

Abstract: A method of adjusting multiple tools on a common mount includes the interrogating first and second actual target positions on at least one workpiece. A variance is determined between the first and second actual target position and first and second theoretical target positions. At least one of the first and second tools is moved relative to the other of the first and second tools from the first and second theoretical target positions to a desired spacing respectively aligned with the first and second actual target positions.

Abstract: A system, method, and apparatus are discussed for a passive optical camera-based system to detect a presence of one or more vehicles with one or more cameras. A detection algorithm is applied to recognize of the presence of the one or more vehicles using one or more imaging processors and the one or more cameras to detect fluctuations in light intensity from scattered light and/or reflections off of that vehicle. Those scattered light and/or reflections are captured in images contained in a set of frames from the one or more cameras.

Abstract: A steering system for a vehicle includes a housing, an input shaft, and a hybrid shaft support. The housing defines an opening. The input shaft is configured to rotate about an input axis. The hybrid shaft support may be disposed at the opening and configured to couple the input shaft to the housing. The hybrid shaft support may also be configured to support the input shaft as the input shaft rotates. The hybrid shaft support further includes a cylindrical member and a flexible seal over-molded onto the cylindrical member.

Abstract: A vehicle includes a low-voltage battery electrically coupled to a powertrain, and a computer programmed to put the vehicle in a minimal risk condition in response to a state of charge of the low-voltage battery falling below a threshold. The vehicle may further include a DC/DC converter electrically coupled to the powertrain and to the low-voltage battery, and a high-voltage battery electrically coupled to the powertrain.

Abstract: A system for use in testing ultrasound systems that includes a tissue mimicking phantom and computer-readable instructions that are configured to automatically compare images of the phantom obtained by the ultrasound system to one or more reference images (e.g., indicating how the obtained images should appear) and provide output that assists personnel in assessing the operationality of the ultrasound system. Any appropriate digital recording device may be used to digitally store signals received from outputs of the ultrasound system for use by the image comparison module on a real-time basis or after full acquisition of the phantom images.

Abstract: A method of presenting a soundscape in an environment space using a plurality of controllable speakers arranged the space is provided. A spatial audio engine may access a spatial audio file defining a digital representation of the soundscape, including (a) a plurality of sound elements to be output in the space over time, (b) sound playback location information defining location(s) for each sound element to be output, and (c) timing information defining time(s) for each sound element to be output. The spatial audio engine may also access speaker location information for each speaker in the space. For each sound element to be played in the space over time, the spatial audio engine may correlate the sound playback location information with the speaker location information to identify one or more of the speakers to be controlled to output audio signals associated with the respective sound element.

Abstract: The INTERIOR COMFORT HVAC USER-FEEDBACK CONTROL SYSTEM AND APPARATUS transforms qualitative feedback received from a user/occupant into a “comfort map,” or modifications thereto, a comfort map being defined at least in part by one or more comfort event windows. The comfort map data is used to determine a temperature setpoint sequence that avoids regions of the map corresponding to known and/or predicted regions of user discomfort.

Abstract: A fire protection sprinkler including a frame body having an inlet, an outlet, defining an internal passageway extending between the inlet and the outlet along a sprinkler axis. A dome shaped deflector member is centered, axially aligned with the sprinkler axis, and spaced from the outlet. The deflector member has an outer surface and an inner surface that preferably includes: a peripheral region, a central region and an intermediate region. The intermediate region includes a primary deflecting surface, and a secondary deflecting surface that is surrounded by the primary deflecting surface. The deflector member provides for generation of a non-circular spray pattern defined by four zones of fluid density concentrically formed about the sprinkler axis.

Abstract: A disposable syringe for use with a pneumatic driver. The syringe has an elongated barrel with a cap secured to the proximal end that has a through bore. A filter is secured to the cap interior of the barrel that covers an opening in the through bore interior of the barrel. The pneumatic driver includes a source of pressurized air; a support for receiving and locating the syringe; and a supply block configured to receive pressurized air from the source and having an outlet for delivery of pressurized air. The supply block is movable between a first position spaced apart from the cap of the syringe and a second position in contact with the cap with fluid communication being established between the outlet of the supply block and the through-bore in the cap.

Abstract: An aircraft wing system and method comprising: an aircraft wing including upper and lower surfaces, and a leading edge between the upper and lower surfaces; a fairing coupled to the leading edge; and actuation means configured to move the fairing relative to the aircraft wing between a stowed position, a first deployed position, and a second deployed position. The stowed position is when the fairing is in contact with the aircraft wing and serves as a continuation of the aircraft wing. The first deployed position is when the fairing is in contact with the aircraft wing and located below the stowed position. The second deployed position is when the fairing is spaced apart from the leading edge to create a gap between the fairing and the leading edge.

Abstract: A prosthetic apparatus and system for a trans-tibial amputee consisting of a post-operative interface socket that can be adjusted to accommodate the size of an amputated limb and changes in size of the limb. The prosthetic apparatus includes a lower socket into which the post-operative socket can be inserted, the lower socket being adjustable for changes in size of the post-operative interface socket. The post-operative socket also has a removable upper back shell that immobilizes movement of the knee during an initial recovery phase but can be removed to allow the knee of the amputee to flex.

Abstract: An aircraft wing system and method comprising: an aircraft wing; an edge device coupled to a leading edge or trailing edge of the aircraft wing; a drive shaft rotatable about its axis; a crank arm, a first end of the crank arm being coupled to the drive shaft, and a second end of the crank arm opposite to the first end being coupled to the edge device; and a gear system (e.g. a strain wave gear system) coupled between the drive shaft and the first end of the crank arm.

Abstract: Installation tools and methods for installing a sprinkler within an opening or through hole in a wall or ceiling of an area to be protected. The tools include a reference surface and a gauge to be contacted by a sprinkler frame. The reference surface locates the gauge to define an operative position for the sprinkler frame and its operational components within a through hole of a wall.