Abstract: There are disclosed mammary gland tissue-specific expression systems using the promoter site for the &bgr;-casein gene of Korean native goats, by use of which physiological activating substances can be produced. In each of the expression systems, that is, novel plasmids pGbc, pGbc_L and pGbc_S (deposition Nos. KCTC 0515BP, 0514BP and 0513BP, respectively), a &bgr;-casein gene expression-regulating region, a physiological activating substance gene and a termination-regulating region are linked. Human granulocyte colony stimulating factor (hG-CSF) or human granulocyte macrophage colony stimulating factor (hGM-CSF) can be produced in HC11 cells, a mouse mammary gland tissue-derived cell line, and in the milk secreted from the transgenic mice by use of a hG-CSF or hGM-CSF gene-carrying pGbc, pGbc_L or pGbc_S in transfection into cell and microinjection to mouse. The proteins are those which experience the posttranslational modification and maintain their normal activity in the human body.

Abstract: There are disclosed mammary gland tissue-specific expression systems using the promoter site for the &bgr;-casein gene of Korean native goats, by use of which physiological activating substances can be produced. In each of the expression systems, that is, novel plasmids pGbc, pGbc_L and pGbc_S (deposition Nos. KCTC 0515BP, 0514BP and 0513BP, respectively), a &bgr;-casein gene expression-regulating region, a physiological activating substance gene and a termination-regulating region are linked. Human granulocyte colony stimulating factor (hG-CSF) or human granulocyte macrophage colony stimulating factor (hGM-CSF) can be produced in HC11 cells, a mouse mammary gland tissue-derived cell line, and in the milk secreted from the transgenic mice by use of a hG-CSF or hGM-CSF gene-carrying pGbc, pGbc_L or pGbc_S in transfection into cell and microinjection to mouse. The proteins are those which experience the posttranslational modification and maintain their normal activity in the human body.

Abstract: A transgenic goat zygote is developed from a goat zygote comprising a nucleic acid construct containing a nucleotide sequence of a goat &bgr;-casein promoter and a nucleotide sequence encoding hG-CSF, which produces milk containing a high concentration of biologically active hG-CSF.

Abstract: A method for distributively managing the CRL in a certifying system to certify the validity of a subscriber in an open communications network such as Internet, includes the steps of registering the certificate policy statement for the CRL by determining the distribution interval of the CRL; setting the structure of the certificate of the subscriber to issue the certificate according to the registered certificate policy statement; attesting the certificate by applying the distribution point mechanism according to the distribution interval to the CRL; and revoking the certificate by using the distribution points to revise the CRL displayed.

Abstract: A system and method for providing information services using a bar code at a wireless terminal, which is capable of receiving a variety of information services including user authentication through a network by receiving a bar code from a wireless terminal over the Internet and reading the transmitted bar code from a bar code system of a member store. The bar code system is constructed such that various services may be offered through a bar code system associated with a bar code issue server using a bar code transmitted to the wireless terminal through the Internet. Accordingly, the information service system and method are provided for various on line business services while not substantially modifying conventional bar code systems. Also, a bar code service can be offered through the wireless terminal, thereby allowing for excellent mobility compared with the conventional bar code system.

Abstract: A points trading service method is provided and includes the steps of (a) collecting information on a customers' trading points total from a member shop and storing the information in a customer database; (b) receiving information on a points trade request from a customer; and (c) trading points with other customers according to the points trade request. According to the method of the present invention, a customer can redeem a small amount of points into cash and exchange the points the user does not want to use for other points. Upon collecting trading points, a customer can also use the cyber money or points at a plurality of member shops. Therefore, when the method according to the present invention is applied, Internet business marketing and cyber money use can be extended and promoted.