Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

Abstract: Systems and methods for sharing radio resources on an air interface. One embodiment comprises an access network element (e.g., base station) that stores a plurality of sharing patterns each comprising a different mapping of the radio resources on a physical layer of the air interface between Machine-Type Communications (MTC) and non-MTC. The access network element identifies a resource sharing window, receives input indicating conditions in the access network, selects a sharing pattern from the plurality of sharing patterns in the pattern database for the resource sharing window based on the conditions in the access network, schedules MTC transmissions on the air interface at a beginning of the resource sharing window based on the sharing pattern, and schedules non-MTC transmissions on the air interface at the beginning of the resource sharing window based on the sharing pattern.

Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.

Abstract: Systems, methods, and software for handling random access procedures. In one embodiment, an access network element that stores an MTC reservation pattern that defines at least one MTC-On interval where MTC is allowed, and defines at least one MTC-Off interval where MTC is prohibited. During an MTC-On interval, the access network element receives preambles for random access procedures from MTC devices, and performs the random access procedures for the MTC devices in response to the preambles. The access network element detects a transition from the MTC-On interval to a next MTC-Off interval, and determines whether the random access procedures for the MTC devices will complete before the transition. If not, the access network element modifies the MTC reservation pattern to expedite completion of the random access procedures.

Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.

Abstract: Disclosed are methods and compositions for enriching nucleic acid fragments from a sample that include one or more target region of interest. In certain aspects, a sample of double stranded nucleic acid fragments having a strand-linking adapter at one end and a non-strand-linking adapter at the other end are denatured and contacted with capture probes specific for a target sequence of interest. Capture probe-bound fragments are isolated from the sample, e.g., using a solid substrate specific for the binding moiety on the capture probes, and are renatured for downstream processing, thus maintaining the original double-stranded region. This enrichment process does not require amplification and as such maintains the nucleic acids in their native states. The disclosed enrichment process and compositions are suitable for analyzing nucleic acids that are fragmented and/or damaged, e.g., cell-free DNA such as circulating tumor DNA, as well as nucleic acids that are many kilobases in length.

Abstract: The present invention provides a method of dynamical adjustment for manufacturing a thermally treated steel sheet.

Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.

Abstract: A method of configuring transmissions over a radio bearer between a base station and user equipment of a wireless telecommunication network, a base station, user equipment, a radio network controller and computer program products are disclosed.

Abstract: The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.

Abstract: Compositions, methods and systems are provided for the loading of extended polymerase-nucleic acid complexes onto substrates. Primed polymerase-template complex comprising a polymerase enzyme and a circular nucleic acid template comprising a double stranded region connected at each end by a single-stranded hairpin region are provides in which the circular nucleic acid template comprises at least one reversible pause point. Nucleic acid synthesis is carried out such that a nascent strand is synthesized up to the reversible stop point producing an extended polymerase-template complex. The extended polymerase-template complex is then attached to a substrate. Such attached complexes can be used for single molecule sequencing in which the nucleic acid synthesis is re-initiated such that nucleic acid synthesis continues past the reversible stop point.

Abstract: A solid oxide fuel cell (SOFC) system including a steam reformer, a hydrogen purification system, a pre-reformer, and a solid oxide fuel cell.

Abstract: Disclosed is an alternative fuel fueling station useful for fueling both electrical and hydrogen alternative fuel vehicles simultaneously. The alternative fuel fueling station includes a solid oxide fuel cell, an electrical conduit, and a compressed hydrogen conduit, such that the alternative fuel fueling station can fuel both the electrical and hydrogen alternative fuel vehicles simultaneously.

Abstract: A solid oxide fuel cell (SOFC) system including a steam reformer, a hydrogen purification system, a pre-reformer, and a solid oxide fuel cell.

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

Abstract: A method for producing a purified carbon dioxide product suitable for EOR and surplus electricity uses a vaporous hydrocarbon feed and a SOFC system. A SOFC system includes a condensate removal system, an acid gas removal system, a hydrodesulfurization system, a sorption bed system, a pre-reformer, a solid oxide fuel cell, a CO2 separations system and a CO2 dehydration system operable to form the purified carbon dioxide product, where the SOFC system is operable to produce surplus electricity from the electricity produced by the solid oxide fuel cell. A method of operating the pre-reformer to maximize the internal reforming capacity of a downstream solid oxide fuel cell uses a pre-reformer fluidly coupled on the upstream side of a solid oxide fuel cell. A method of enhancing hydrocarbon fluid recovery from a hydrocarbon-bearing formation using a SOFC system.

Abstract: An equalizer and method is implemented to improve the performance of a communication system based on multi-level amplitude modulation schemes. The equalizer may include a linear equalization circuit including a plurality of time delayed taps and configured to receive an input signal and generate an output signal. The equalizer may further include a nonlinear circuit configured to receive signals from at least a portion of the time delayed taps and generate at least a portion of a difference between the signals, the output signal based at least in part on the difference.

Abstract: Disclosed is an alternative fuel fueling station useful for fueling both electrical and hydrogen alternative fuel vehicles simultaneously. The alternative fuel fueling station includes a solid oxide fuel cell, an electrical conduit, and a compressed hydrogen conduit, such that the alternative fuel fueling station can fuel both the electrical and hydrogen alternative fuel vehicles simultaneously.

Abstract: Compositions, methods and systems are provided for isolating DNA having a modified or unnatural base. Circular DNA fragments, each comprising a double stranded DNA central region and single stranded regions on the ends of the double stranded regions, are obtained. Some of the fragments have one or more modified or unnatural base. The DNA fragments are treated with a primer and a polymerase such that the polymerase extends the primer to copy at least one of the strand of the double stranded region. This results in rendering the other strand single stranded. A binding protein or antibody that is specific to the modified or unnatural base is then used to isolate strands containing the modified or unnatural bases. Methods for loading such complexes onto substrates and for single molecule sequencing of such complexes are also provided.

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogs into active site regions and for coordinating with the nucleotide analogs in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.