Abstract: A quick action nut and a threaded fastener assembly including the quick action nut are proposed in the present invention. The quick action nut includes a nut housing, a control operating member, and at least two moving blocks movably connected inside the nut housing. A first through hole having a first hole axis is provided on the nut housing for a screw to pass through. Radial slide ways are provided on the nut housing for the moving blocks to move in a direction perpendicular to the first hole axis. The radial slide ways and the moving blocks have the same quantity, are matched in a one-to-one correspondence, and are distributed around the first hole axis. Threads are provided on the moving block to form or not form a threaded hole structure that is matched with the screw.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Abstract: Provided herein are sulfo n-hydroxysuccimidyl ester (sulfo-SE) linked peptides, methods of synthesis thereof, and methods of using such peptides for labeling of biomolecules. In particular, peptides comprising non-alkyl group such as serine, threonine, cysteine, tyrosine, glutamic acid, and aspartic acid are stably modified (e.g., without autoreactivity) with a sulfo-SE group and used to label or otherwise modify biomolecules.

Abstract: Disclosed herein are compositions and methods for stabilizing a benzothiazole luciferin analog such as D-luciferin and 6-amino-D-luciferin. The compositions may include the benzothiazole luciferin analog, a thionucleobase compound of formula (I), and a liquid medium, in which the thionucleobase is present in an amount effective to stabilize the luminogenic composition against decomposition. The methods provided herein may stabilize the benzothiazole luciferin analog against decomposition by contacting the benzothiazole luciferin analog with an effective amount of the thionucleobase compound in the presence of a liquid medium. Also provided herein is a kit containing the composition.

Abstract: A threaded fastening assembly includes a nut body and a screw body. The internal thread of the nut body includes a nut thread crest and a nut thread root. The external thread of the screw body includes a screw thread crest, a screw thread root, a fourth side wall located on one side of the screw thread crest, and a fifth side wall located on the other side of the screw thread crest. A locking notch located on one side of the nut thread crest is arranged on the internal thread. The locking notch includes a first side wall and a bottom wall. One end of the first side wall is connected to the nut thread crest, and the other end of the first side wall is connected to one end of the bottom wall. The bottom wall is spirally distributed around the axis of the nut body.

Abstract: Provided herein are methods for the real-time monitoring of an intracellular event or response. In particular, the methods provided herein monitor the conversion of a pro-substrate to a substrate for a protein sensor as a result of an intracellular event or response.

Abstract: Provided herein are materials and methods for performing bioluminescent assays using a bifunctional probe. In particular, the present disclosure provides compositions and methods for detecting and/or quantifying a biomolecule and/or assaying a cellular process associated with the biomolecule using a bifunctional probe capable of binding the biomolecule and generating a bioluminescent and/or fluorescent signal.

Abstract: The present disclosure provides a positioning device, including a laser projector, and a servo system for driving the laser projector and collecting a coordinate data. The present disclosure further provides a positioning method and a color film coating machine, including a warehouse and a marble platform arranged in the warehouse, wherein further includes a positioning device, the positioning device includes a laser projector and a servo system for driving the laser projector and collecting a coordinate data, a projection direction of the laser projector is opposite to a surface of the marble platform. Compared with the prior art, by knowing the current position of the laser projector to output the corresponding coordinates, the corresponding target foreign objects can be quickly found on the marble platform according to the coordinates, cleared in time, the inspection time is saved, and the product yield is improved.

Abstract: Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Abstract: The present invention provides an assay for detection of oxidized glutathione (GSSG).

Abstract: Provided herein are compounds, compositions, and methods for delivering biomolecules to cells. In particular, the present disclosure provides modified polyamine polymers, including polyamine polymers having fluorinated substituents.

Abstract: Provided herein are systems and methods for the detection of an analyte or analytes in a sample. In particular, the present disclosure provides compositions, assays, and methods for detecting and/or quantifying a target analyte using a bioluminescent complex comprising substrates, peptides, and/or polypeptides capable of generating a bioluminescent signal that correlates to the presence, absence, or amount of the target analyte.

Abstract: Methods using photothermal nanoparticles in rapid nucleic acid amplification and photothermal nanoparticles include forming a reaction mixture comprising a nucleic acid template, a polymerase enzyme, and a heating media. The heating media comprises a plurality of photothermal nanoparticles suspended in a solution, fabricated on a microchip, or on a surface of a well in a multi-well plate. The photothermal nanoparticles are nanoparticles having a particular geometric shape and comprise a single component or a multi-component. The plurality of photothermal nanoparticles are further categorized by surface plasmon resonance with a resonance wavelength in a particular range to convert energy absorbed from a light source to sufficiently heat a volume of a composition for the transcription of the new nucleic acid strand. The particular geometric shape of the photothermal nanoparticles is selected from the group consisting of: polyhedral, spheroid, torus, and hollow shapes.

Abstract: A medical device and a storage box for a medical article are provided, where the medical device at least includes a main case having at least two exposed chamber outer walls and an accommodation compartment for accommodating a medical article. The at least two exposed chamber outer walls enclose an accommodation chamber to accommodate a functional module. The accommodation compartment is disposed on the main case and adjacent to the accommodation chamber. The accommodation compartment includes at least one exposed compartment outer wall, and the at least one exposed compartment outer wall is connected to the at least one chamber outer wall of the main case. The medical device enables the medical article to be well accommodated in the accommodation compartment, and the accommodation compartment can be flexibly configured onto the medical device to decrease the space occupied by the accommodation compartment.

Abstract: Described are pro-coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays or fragment complementary luciferase. The disclosed pro-coelenterazine analogues provide increased serum stability for live cell assays, and are capable of tuning the brightness and assay windows as needed for the applications.

Abstract: Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Abstract: A toilet capable of measuring body temperature, includes a toilet base and a machine body connected to the toilet base, wherein the machine body is connected to a toilet seat. The toilet seat is rotatable relative to the machine body and covers the toilet base. A body temperature detection device is provided on the machine body. In the present invention, the combination of human body temperature measurement and the toilet can enable people to measure body temperature while using the toilet. The temperature measurement has the advantages of quick operation, convenience, and being close to life.