Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

Abstract: The present disclosure provides a positioning device, including a laser projector, and a servo system for driving the laser projector and collecting a coordinate data. The present disclosure further provides a positioning method and a color film coating machine, including a warehouse and a marble platform arranged in the warehouse, wherein further includes a positioning device, the positioning device includes a laser projector and a servo system for driving the laser projector and collecting a coordinate data, a projection direction of the laser projector is opposite to a surface of the marble platform. Compared with the prior art, by knowing the current position of the laser projector to output the corresponding coordinates, the corresponding target foreign objects can be quickly found on the marble platform according to the coordinates, cleared in time, the inspection time is saved, and the product yield is improved.

Abstract: Provided herein are methods for the real-time monitoring of an intracellular event or response. In particular, the methods provided herein monitor the conversion of a pro-substrate to a substrate for a protein sensor as a result of an intracellular event or response.

Abstract: Described are pro-coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays or fragment complementary luciferase. The disclosed pro-coelenterazine analogues provide increased serum stability for live cell assays, and are capable of tuning the brightness and assay windows as needed for the applications.

Abstract: A smart toilet having a function of human blood oxygen detection is provided. The smart toilet includes a toilet base and a machine body connected to the toilet base. The machine body is provided with a controller and the following modules which are electrically connected to controller respectively, i.e., human blood oxygen detection module, display or voice prompt module, and power supply module which is used to supply power. After processing, the controller obtains the human blood oxygen saturation value based on the electrical signal transferred from the human blood oxygen detection module. Also, the controller controls the display or voice prompt module to output the human blood oxygen saturation value. The present invention combines the toilet with the human blood oxygen detection, such that it is easy for the user to know about his/her blood oxygen condition in real time.

Abstract: A smart toilet with a function of electrocardiographic lead monitoring, includes a toilet base, and further includes a body connected to the toilet base. The body is provided with a controller, and an electrocardiographic lead monitoring module, a display or voice prompt module, a power supply module for supplying power, which are electrically connected to the controller respectively. The human body ECG signal obtained by the electrocardiographic lead monitoring module is processed by the controller, to obtain human body electrocardiographic lead data, and the display or voice prompt module is controlled to output the data. The invention combines the electrocardiographic monitoring function with the toilet, which enables people to know electrocardiographic data quickly and conveniently while using the toilet, so as to understand their own heart health, thereby detecting and dealing with possible diseases as soon as possible.

Abstract: A smart toilet with a function of human blood pressure detection, the smart toilet includes a toilet base. The smart toilet further includes a body connected to the toilet base. The body is provided with a controller and a human blood pressure detection module, a display or voice prompt module, a key input module for sending back operation information of the user to the controller, and a power supply module for supplying power, which are electrically connected to the controller respectively. The controller processes the electrical signal transmitted by the human blood pressure detection module to obtain the blood pressure value of the human body, and controls the display or voice prompt module to output the blood pressure value. The human blood pressure can be conveniently and rapidly detected while the users use the toilet every day, so that abnormal blood pressure can be discovered early.

Abstract: A smart toilet with a function of detecting the body composition is provided. The smart toilet includes a toilet base and a machine body connected to the toilet base. The machine body is provided with a controller and the following modules that are electrically connected to the controller respectively, i.e., the body composition detecting module, the display or voice prompt module, the key input module which is used to send the operating information of the user and physical parameters of the user back to the controller, and the power supply module which is used to supply power. After processing, the controller obtains the impedance value of human body based on an electrical signal acquired by the body composition detecting module. The controller compares the impedance value to physical parameters of the user to conduct a final processing.

Abstract: A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

Abstract: Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.

Abstract: Provided are pro-substrates useful in assays of living cells. The pro-substrates can be used to detect the presence or absence of enzymes, such as luciferase, in living cells. The pro-substrates can be coelenterazine derivatives or analogs.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Abstract: The present invention provides an assay for detection of oxidized glutathione (GSSG).

Abstract: The present invention provides an assay for detection of oxidized glutathione (GSSG).

Abstract: Provided are labeling reagents and methods of using the reagents for cell uptake measurements. The labeling reagents can be quinone-masked probes including fluorophores and/or luminophores.

Abstract: Thienopyrrole compounds that may inhibit Oplophorus-derived luciferases are disclosed, as well as compositions and kits comprising the thienopyrrole compounds, and methods of using the thienopyrrole compounds.

Abstract: The invention provides coelenterazine derivatives which are substrates for a non-luminescent enzyme and a pro-substrate for a luminescent enzyme. The invention also provides a method of using the derivatives.