Abstract: A surgical fluorescence stereomicroscope (1) for detecting and operating on fluorescing areas of an object field (7) is described, which microscope encompasses a first illumination device (2) for irradiating the object field (7) with light in an excitation wavelength region (E). The surgical fluorescence stereomicroscope (1) further encompasses an observation beam path (21) for guiding the reflected and emitted light received from the object field (7), and a first observation filter (9) in the observation beam path (21) which is transparent in the excitation wavelength region (E) and in a fluorescence wavelength region (F). According to the present invention, means (10a to 10c) for controllable attenuation in the excitation wavelength region (E) are additionally arranged in the observation beam path.

Abstract: A scanning microscope is described, having an illumination unit for emitting an illumination light beam, an objective for generating an elongated illumination focus in a specimen to be imaged, and a scanning apparatus for moving the illumination focus over a target region of the specimen to be illuminated by modifying the direction of incidence in which the illumination light beam is incident into an entrance pupil of the objective. The scanning apparatus directs the illumination light beam onto a sub-region of the entrance pupil offset from the pupil center in order to incline the illumination focus relative to the optical axis of the objective, and modifies the direction of incidence of the illumination light beam within that sub-region in order to move the illumination focus over the target region to be illuminated.

Abstract: An illuminating unit that is a part of a microscope for forming an image of a specimen being an observation object, that is located above a stage on which the specimen is placed, and that applies transmitting illumination light to the specimen includes a light source unit including a light source that generates the transmitting illumination light, and a condenser unit that includes a condenser lens for collecting and applying the transmitting illumination light emitted by the light source unit onto the specimen, and that is detachably connected to the light source unit.

Abstract: A tunable multiple laser pulse scanning microscope and a method of operating the same is described, applying two pulsed laser beams with distinct wavelengths incident on a scanning spot of a sample to be imaged simultaneously or at a specific time delay. The microscope comprises at least two pulsed laser light sources emitting laser light of distinct wavelengths, an acousto-optic tunable filter (AOTF) for tuning at least one of the laser pulses, a delay stage provided upstream of the AOTF, and an actuator for moving delay stage depending on the time delay. As a result, the wavelength of at least one type of pulses is tuned, and the delay between at least two pulses of distinct wavelengths is adjusted.

Abstract: A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set.

Abstract: Methods and a computer program product for applying deconvolution to spatial light interference microscopy for resolution enhancement with respect to the diffraction limit in two and three dimensions. By exploiting the sparsity properties of the phase images, which is prominent in many biological imaging applications, and modeling of the image formation via complex fields, the very fine structures can be recovered which were blurred by the optics. The resolution improvement leads to higher accuracy in monitoring dynamic activity over time. Experiments with primary brain cells, i.e. neurons and glial cells, reveal new subdiffraction structures and motions. This new information can be used for studying vesicle transport in neurons, which may shed light on dynamic cell functioning. Finally, the method may flexibly incorporate a wide range of image models for different applications and can be utilized for all imaging modalities acquiring complex field images.

Abstract: A microscope includes an illumination unit for illuminating a mask at a predetermined non-axial illumination angle, an imaging unit for imaging an aerial image of the mask within a predetermined defocus region, and an imaging field stop, in which as a result of the lateral displacement of the aerial image depending on the position within the defocus region and on the non-axial illumination angle, the opening of the imaging field stop is dimensioned such that the aerial image is either completely encompassed or circumferentially cut within the defocus region. A method for characterizing a mask having a structure includes illuminating the mask at at least one illumination angle using monochromatic illumination radiation such that a diffraction image of the structure is created, recording the diffraction image, establishing the intensities of the maxima of the adjacent orders of diffraction, and establishing an intensity ratio of the intensities.

Abstract: One embodiment provides light along an optical axis. It comprises a substrate and at least one array of multiple LED chips without individual packaging supported by the substrate. The LED chips emit light within different wavelength ranges and are distributed laterally with respect to the axis over an area, the LED chips having light emitting surfaces for emitting light in directions transverse to the area. An optical element adjacent to the light emitting surfaces of the LED chips in the at least one array collects and directs light emitted by the LED chips of the at least one array along the axis towards a target. Another embodiment is directed to a method for providing multiple wavelength light for fluorescent microscopy using the above system. Electric current is supplied to the multiple LED chips, causing them to emit light of multiple wavelengths.

Abstract: A modulation contrast microscope that affords good view of sperm in ICSI, in particular, good view during sperm manipulation in ICSI, by improving contrast of the end portion of the tail includes the modulation contrast microscope comprises an aperture member having a partial aperture disposed at or near the front focal plane of a condenser lens, and a modulator disposed at a plane substantially conjugate with the aperture member, at or near the rear focal plane of a first objective lens or a conjugate plane thereof. The transmittance T(%) of a region of the modulator, corresponding to the partial aperture, satisfies the condition 1 £ T £ 8. Good viewing for ICSI sperm manipulation can be obtained as a result.

Abstract: A laser scanning microscope has an illumination beam path and a detection beam path. A beamsplitter is provided which reflects the illumination light in direction of the sample and transmits the detection light in direction of the detection arrangement. An additional beamsplitter is provided for reflecting the illumination light and for transmitting the detection light, this additional beamsplitter being arranged in the illumination beam path downstream of the first beamsplitter in the illumination direction, and this additional beamsplitter substantially transmits the illumination light reflected at the first beamsplitter and the detection light, but acquires a wavelength range substantially different from the first beamsplitter with respect to its reflectivity.

Abstract: This microscope apparatus is provided with the following elements: an objective lens; a CCD that constructs an image of a sample S; an illumination intensity change unit for adjusting an intensity of excitation light; an sensitivity adjustment unit for adjusting a detection sensitivity by the CCD; a galvanometer mirror for changing a light focusing position of excitation light at a pupil position of the objective lens; a storage unit that stores, for each observation method, a predetermined intensity, a predetermined detection sensitivity, and the light focusing position; and a control unit that switches the observation method, reads out the intensity, the detection sensitivity, and the light focusing position according to the observation method based on a synchronization signal synchronized with a frame signal for constructing an image, and controls the illumination intensity change unit, the sensitivity adjustment unit, and the galvanometer mirror.

Abstract: A collimator lens is configured with a double convex single lens formed with a resinous material, for collimating a light flux emitted from a light source. At least one of surfaces of the collimator lens is formed as an aspherical surface. The collimator lens satisfies following conditions: 2<|R1/R2|<10; NA>0.6, where R1 is a curvature radius of a first surface, which is a lens surface on a side of the light source of the collimator lens, R2 is a curvature radius of a second surface, which is a lens surface on a side opposite to the first surface of the collimator lens, and NA is a numerical aperture on the side of the light source of the collimator lens.

Abstract: A device for examining and manipulating microscopic objects with a microscope having a light source that serves to illuminate the object, and which generates an illumination light beam that runs along and illumination beam path, that can be guided over or through the object by means of a beam deflector, with a detector to detect light emitted from the object that runs along the detection beam path, with a primary beam splitter, and with a light source, which generates a manipulation light beam that runs along an illumination beam path, that serves to manipulate the object.

Abstract: A 3D localisation microscopy system, 4D localisation microscopy system, or an emitter tracking system arranged to cause a phase difference between light passing to or from one part of the objective relative to light passing to or from another part of the objective, to produce a point emitter image which comprises two lobes, a separation between which is related to the position of the emitter relative to the objective of the imaging system, and in the 4D system a further property of which image or of said light to or from the objective is related to another location independent property of the emitter.

Abstract: An apparatus for and method of performing orthogonal light sheet microscopy (OLM) and computer optical tomography (COT) simultaneously in a single device are provided. The dual-mode imaging microscope allows for the use of both OLM and COT in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples, and improved imaging results. In addition, the device will reduce the high costs and space requirements associated with owning two different imagers (OLM and COT).

Abstract: Systems and methods are provided for illuminating a surface to be observed microscopically using a retractable beamsplitter. The retractable beamsplitter allows the use of coaxial illumination when the beamsplitter is positioned in the operator's line of sight. The retractable beamsplitter allows the use of non-coaxial illumination without reducing the amount of illumination that reaches the operator when the beamsplitter is retracted from the operator's line of sight. As a result a single system can be used effectively to provide various types of illumination.

Abstract: An apparatus for and method of performing light sheet microscopy (LISH) and light scanning microscopy (RAPS) in a single device are provided. The dual-mode imaging microscope allows for the use of both LISH and RAPS in a single instrument. This dual-mode device will allow researchers to have access to both types of microscopy, allowing access to the widest possible selection of samples. In addition, the device will reduce the high costs and space requirements associated with owning two different microscopes (LISH and RAPS).

Abstract: A structured-illumination module included in a 3D-structured-illumination-based fluorescence microscope, the structured-illumination module comprising: a structured-illumination-module frame; a beam-alignment module including a central tilt mirror coupled to an underside of a top horizontal plate of the structured-illumination-module frame; a set of directional mirrors, one of which receives, at a given point in time, input, polarized, coherent light reflected from the central tilt mirror; three sets of beam splitters, on three arms of the structured-illumination-module frame, the each splits an incident illumination beam, reflected to the set of beam splitters from a directional mirror of the set of directional mirrors, into a coherent beam triplet; and a phase-shift module that receives a beam triplet, at a given point in time, generated by one of the sets of beam splitters and reflected from the beam-alignment module and that introduces a desired relative phase relationship among the beams of the beam trip

Abstract: A microscope objective comprises in order from an object side, a first lens group, a second lens group, and a third lens group. The first lens group includes a first cemented lens, and at least one positive single lens, the second lens group includes a second cemented lens, and the third lens group includes a first lens component and a second lens component. A positive lens and a meniscus lens are cemented in the first cemented lens. A surface nearest to an image side of the first lens component is a concave surface, and a surface nearest to the object side of the second lens component is a concave surface. The first lens component and the second lens component are disposed such that the both concave surfaces are face-to-face, and the following conditional expression (1) is satisfied. 0.5<(n0/nlo)/NAob<0.

Abstract: An illumination device includes a phase-modulation type spatial light modulator that is arranged at a position optically conjugate to a pupil position of an illumination lens between a light source and the illumination lens for irradiating light emitted from the light source on a sample, and a pupil projecting optics system for projecting at least one area within the pupil of the illumination lens onto a plurality of different areas of the spatial light modulator.

Abstract: A microscope for conventional fluorescence microscopy (epi-fluorescence) and for total internal reflection microscopy is described. A first light source emits conventional fluorescent illumination light along a first illumination path and a second light source emitting evanescent illumination light along a second illumination path that differs from the first illumination path. An objective emits light onto an object to be viewed. A beam combiner directs the two lights into the objective while keeping their beam paths geometrically separated. The beam combiner comprises at least two spatially separated first zones for coupling in the conventional fluorescent illumination light and at least two spatially separated second zones for coupling in the evanescent illumination light. The first and second zones are adapted in their size and position to objective pupils of different objectives.

Abstract: An operating microscope system (200) for ophthalmology, particularly vitrectomy, is proposed, which comprises an operating microscope (10) with a microscope illumination (15) and a wide-angle device (20) arranged on the objective side in front of the operating microscope (10), the wide-angle device (20) comprising a wide-angle lens (21) arranged on the object side of the wide-angle device (20) and a prism arrangement (22) for righting the image, arranged on the microscope side of the wide-angle lens (21), wherein the wide-angle device (20) comprises an illuminating unit (25) which is arranged so as to illuminate an object (40) arranged in front of the wide-angle lens (21) through the wide-angle lens (21) by means of an illuminating light (29).

Abstract: The invention is directed to an optical arrangement for photomanipulation of a sample comprising a sample holder for receiving the sample, an illumination device comprising an illumination light source and an illumination beam path for illuminating the sample with a light sheet. It further comprises a detection device for detecting light that is radiated from the sample and imaging optics which image the sample on the detection device by means of an imaging objective in an imaging beam path, wherein the light sheet is substantially planar in the focus of the imaging objective, and wherein the imaging objective has an optical axis which intersects the plane of the light sheet at an angle different from zero. Further, the arrangement also has means for photomanipulation of the sample.

Abstract: The invention relates to a device for increasing the depth discrimination of optical imaging systems. Such a device comprises means for structuring illumination light in an illumination beam of the optical imaging system by means of a grating-like pattern in a conjugated object plane of the optical imaging system. The device further comprises means for varying the position of the grating-like patterns along an optical axis of the illuminating beam, and means for varying the position of an image of the grating-like pattern on a specimen in an object plane. Such a device is characterized by a grating changer connected to an electronic control system or to a control program of the optical imaging system for automatically changing the grating-like pattern to at least one further grating-like pattern and/or for removing the grating-like pattern from the illuminating beam path.

Abstract: The invention relates to a method for calibrating a deflection unit in a TIRF microscope, by means of which an angle of incidence of excitation light onto a specimen is adjusted, wherein a setting of said deflection unit is adjusted such that the pertaining angle of incidence is definitely greater or definitely smaller than an anticipated critical angle for total reflection of the excitation light on a surface of a used specimen, the angle of incidence is scanned by varying the setting of said deflection unit in the direction of an anticipated critical angle, an intensity of an optical response of the used specimen elicited by the excitation light being measured for each setting of the deflection unit, the intensity of the optical response of the specimen used is measured at least for a number of settings of the deflection unit until the intensity of the optical response of the specimen used traverses a flank, and the setting of the deflection unit pertaining to the flank is stored as the setting for the crit

Abstract: A flat panel light source (100) for a transillumination device of a microscope comprises a plate-shaped light guide (110) having a lower and an upper boundary surface, and at least one lateral surface (113 to 116), and having at least one light-emitting means (120, 122) arranged to radiate light into the light guide (110) from at least two different directions, via at least one lateral surface serving as a light entrance surface, such that the light propagates in the light guide (110) as a result of total reflection, the total reflection being disrupted in defined fashion, by an element (140) abutting against a contact surface at the lower boundary surface of the light guide (110), so that an outcoupling of light occurs on the upper boundary surface of the light guide (110), the planar area of the contact surface being smaller than the planar area of the lower boundary surface.

Abstract: A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A structured phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set.

Abstract: A confocal microscope apparatus comprises a first optical scanning system which obtains a scan image of a sample using a laser beam from a first laser light source, a second optical scanning system which scans specific regions of a sample with a laser beam from a second laser light source that is different from the first laser light source, thereby causing a particular phenomenon, and a beam diameter varying mechanism which can change the beam diameter of the laser beam of at least one of the first optical scanning system and the second optical scanning system. With this configuration, the apparatus further comprises an excitation light intensity distribution calculator which calculates and stores the excitation light intensity distribution along a depth direction on the sample surface from the beam diameter of the laser beam output from the beam diameter varying mechanism.

Abstract: A scanning microscope is provided with a scan unit that scans a sample, the scanning microscope including: a transmissive VPH grating for dispersing light from the sample; and a photodetector for detecting the light diffracted by the VPH grating.

Abstract: A structured illumination apparatus includes a light modulator being disposed in an exit flux of light from a light source and in which a sonic wave propagation path is arranged in a direction traversing the exit flux of light; a driving unit generating a sonic standing wave in the sonic wave propagation path by giving a driving signal for vibrating a medium of the sonic wave propagation path to the light modulator; and an illuminating optical system making mutually different diffracted components of the exit flux of light passed through the sonic wave propagation path to be interfered with each other, and forming interference fringes of the diffracted components on an observational object.

Abstract: In accordance with the invention, there are imaging interferometric microscopes and methods for imaging interferometric microscopy using structural illumination and evanescent coupling for the extension of imaging interferometric microscopy. Furthermore, there are coherent anti-Stokes Raman (CARS) microscopes and methods for coherent anti-Stokes Raman (CARS) microscopy, wherein imaging interferometric microscopy techniques are applied to get material dependent spectroscopic information.

Abstract: A surgical microscope (100) has a viewing optic (101, 104, 115) which allows a viewing person to view the enlarged display of an object region (116) in a viewing area (117). The viewing optic (101, 104, 115) has a continuously adjustable magnification system (104) with which a viewing optic adjusting unit (119) is associated. The surgical microscope (100) has an adjustable illuminating system (150), which provides illuminating light (151) for the object region (116), to illuminate the object region in an illuminated area (152) using illuminating light (151) of adjustable radiation intensity. An illumination system control unit (175) is provided in the surgical microscope (100), the control unit being connected to the viewing optic adjusting unit (119) for receiving information as to the adjusted magnification of the viewing optic (101, 104, 115).

Abstract: A fluorescent microscope for observing multiple fluorescent images includes: a first optical module comprising a first light source for supplying first excitation light having a first wavelength, a first excitation filter for selectively transmitting the first excitation light supplied from the first light source, a first dichroic filter for reflecting the first excitation light having passed through the first excitation filter toward the survey object, an objective lens for condensing the first excitation light reflected by the first dichroic filter and transferring the condensed first excitation light to the survey object, a second dichroic filter for reflecting first radiation light radiated from the survey object, a first radiation filter for selectively transmitting the first radiation light reflected by the second dichroic filter, and a first image acquisition unit for acquiring an image by using the first radiation light having passed through the first radiation filter to be supplied; and a second opti

Abstract: The present invention relates to a special-illumination surgical stereomicroscope (1) for observing an object (6) in an object field (15) under special illumination, the special-illumination surgical stereomicroscope including a surgical illumination light source (7; 7a) for illuminating the object field (15) via an illumination beam path (11) and further including a special-illumination light source. The special-illumination light source is adapted for observation of stimulated emission and includes an excitation light source (8a) for specific excitation of a substance contained in the tissue of the object (6) via an excitation beam path (12a), and a stimulation light source (8b) for stimulating the emission of light from the previously excited substance via a stimulation beam path (12b). A common observation beam path (14) is provided for guiding the light generated by stimulated emission and the reflected surgical illumination light.

Abstract: A stereomicroscope fluorescence system has a light source, a light guide, a focusing lens assembly, a stereomicroscope with two eyepiece receivers, and two eyepiece assemblies. The light guide is operably attached to receive light from the light source to transmit the light to the focusing lens assembly. The focusing lens assembly includes an excitation filter coated on the focusing lens. The eyepiece assemblies each have a barrier filter and are adapted to be mounted on the eyepiece receivers.

Abstract: A microscope system that performs structured illumination includes a light source configured to emit illumination light, an objective lens that irradiates a specimen with the illumination light, a phase-modulation spatial light modulator that has a two-dimensional pixel structure, that is arranged at the pupil conjugate position of the objective lens on an illumination light path between the light source and the objective lens, and that is configured to modulate a phase of the illumination light for each pixel so as to form a fringe illumination pattern on the specimen on the basis of an optical parameter of at least one of the light source and the objective lens.

Abstract: An optical filter 11 is fixed in an optical path in a light generation unit 6 to cut out light in an infrared region. The optical filter 11 do not deviate from the optical path, and infallibly eliminate light in the infrared region which becomes heat radiation. The optical filter 11 cuts out light whose wavelength is longer than a threshold wavelength that is longer than 805 nm and shorter than 815 nm, and eliminates heat radiation in the infrared region, which includes a wavelength (approximately 825 nm) which is substantially a first peak P of the radiant intensity of the xenon lamp 10.

Abstract: A microscope includes: a first epi-illumination light-source unit to perform fluorescence observation; a second transmitted-illumination light-source unit to perform transmission observation, the second transmitted-illumination light-source unit including a light source provided with a light emitting element that emits excitation light and a fluorescent substance that emits fluorescence upon irradiation with the excitation light; and an incidence limiting section configured to limit an incidence of light on the light source from an outside of the second light source unit during a light-off period of the light emitting element. The incidence limiting section is configured to remove an incidence limitation of the light from the outside while the light emitting element is being lit.

Abstract: Oblique-illumination systems integrated with fluorescence microscopes and methods of using oblique illumination in fluorescence microscopy are disclosed. An oblique-illumination system is attached to a fluorescence microscope objective. The oblique-illumination system can be used to illuminate from any desired direction the surface of an object located at a fixed known offset away from a sample solution containing fluorescently tagged targets. Oblique illumination is used to illuminate features of the surface while epi-illumination is used to create fluorescent light emitted from the tagged targets. The combination of oblique illumination of the surface and epi-illumination of the targets enables capture of images of the surface features and the fluorescent targets so that the locations of the targets in the sample can be determined based on the locations of the surface features.

Abstract: A microscope (10) has an aperture arrangement (29) that, in order to limit the dimension of a light beam (41), comprises an aperture opening (37). The size of the aperture opening (37) is adjustable with the aid of a first aperture member (32) and a second aperture member (34). At least one of the two aperture members (32, 34) is movable relative to the other aperture member (32, 34). The aperture members (32, 34) are spaced apart from one another when the aperture opening (37) is closed.

Abstract: A confocal laser microscope has at least one laser whose illumination light is transmitted in direction of the microscope objective by at least one light-conducting fiber. The light-conducting fiber can be plugged in at a housing which preferably comprises the scanning head of the microscope, and a holder is provided which can be plugged into the housing and into which the light-conducting fiber projects and which is provided at its end remote of the fiber with first optics for transmitting the laser light exiting divergently from the fiber in direction of at least partially displaceable collimating optics in the housing. At least second optics are advantageously arranged between the first optics and the collimating optics.

Abstract: Described herein is a kit that includes at least one press for pressing matter that is being observed by a light microscope and a method of pressing the matter using the press while the matter is being observed. The press includes a pestle that is supported between, and for independent motion relative to the objective of the microscope and a sample residing on the stage of the microscope.

Abstract: A scanning microscope includes a light source, illumination optics, and a scanning device for moving the illumination focus across a target region and doing so by varying the direction of incidence in which the illuminating beam enters an entrance pupil of the illumination optics. To incline the illumination focus relative to the optical axis of the optics, the scanning device directs the illuminating beam onto a portion of the entrance pupil that is offset from the center of the pupil and, in order to move the illumination focus across the target region, the scanning device varies the direction of incidence of the illuminating beam within said portion. An observation objective is provided which is spatially separated from the illumination optics and disposed such that its optical axis (O3) is substantially perpendicular to the target region and at an acute angle (?) to the optical axis (O1) of the illumination optics.

Abstract: An instrument and method for scanning at least a portion of a large specimen preferably causes the specimen to move relative to a two-dimensional detector array at a constant speed. The detector array takes one image of the specimen for each line that the detector moves. A controller controls a shutter of the detector array to open to take images and to pass the images to a processor, which is preferably a computer. The instrument takes one partial image of each part of the specimen that is being scanned and then combines those images with other images to produce a contiguous image.

Abstract: Fluorescence is generated from an irradiated point on an inspection surface of a sample and the fluorescence is collected by an objective lens. Here, because of the magnification chromatic aberration of the objective lens 86, the fluorescence going out from the objective lens travels along a path shifted from the irradiation light and changed substantially into a non-scan light by a galvano-scanner. The fluorescence passes through a dichroic mirror and comes into deflection means as 2-dimensional deflection means after light of unnecessary wavelength is removed by a filter. The deflection means is driven in synchronization with the galvano-scanner by a computer and corrects the shift and inclination of the optical axis generated by the magnification chromatic aberration of the objective lens.

Abstract: An apparatus, system and method for microscopy. The apparatus, system and method includes a stage configured to receive an item; a tunable acoustic gradient index of refraction (TAG) lens having a first aspect positioned to image the received item, wherein the first aspect of the TAG lens is configured to have an optical power profile in accordance with an operational frequency of the TAG lens; one or more lenses configured to magnify an image of the received item at a viewing point; and at least one pulsed light source configured to illuminate the received item and to pulse at one or more points within the optical power profile of the TAG lens.

Abstract: A cell counter includes: a sample slide configured to accommodate cells; a housing configured to be inserted into the inside of the sample slide from the outside of the sample slide; an object lens configured to be mounted within the housing, and to image-form a cell image for the cells projected from the sample slide; an image acquisition unit configured to be mounted within the housing together with the object lens, and to acquire the cell image image-formed by the object lens; and a first reflecting mirror provided between the sample slide and the object lens within the housing, and configured to change a projection direction of the cell image projected from the sample slide to the object lens.

Abstract: The present invention relates to a microscope (1), preferably a dental microscope, including a microscope body (15) and a stand (2) formed by a plurality of components to provide a supporting function or to enable positioning of the microscope (1) in the room (11), the microscope body (15) and the stand (2) having cavities (16) therein. It is a feature of the present invention that at least one cavity (16) of the microscope body (15) and/or the stand (2) has a light source (17) provided therein whose light (17a, 17b, 17c) can pass outwardly through passage openings (18, 24, 27).

Abstract: An ultrahigh-resolution fiber-optic confocal microscope has an illumination system; three single-mode optical fibers, each optically coupled to a fiber coupler; a sample support stage arranged to receive illumination radiation from an end of one of the single-mode optical fibers; a detector arranged to receive output radiation from one of the single-mode optical fibers; and a lock-in amplifier electrically connected to the detector and the illumination system. The illumination system is adapted to provide illumination radiation that has a time-varying strength that is correlated with the detector by the lock-in amplifier.

Abstract: A chromatic confocal microscope system and signal process method is provided to utilize a first optical fiber module for modulating a light into a detecting light passing through a chromatic dispersion objective and thereby forming a plurality of chromatic dispersion lights to project onto an object. A second optical fiber module conjugated with the first optical fiber module receives a reflected object light for forming a filtered light, which is split into two filtered lights detected by two color sensing units for generating two sets of RGB intensity signals, wherein one set of RGB intensity signals is adjusted relative to the other set of RGB intensity signals. Then two sets of RGB intensity signals are calculated for obtaining a maximum ratio factor. Finally, according to the maximum ratio factor and a depth relation curve, the surface profile of the object can be reconstructed.