Abstract: An optical member and a microscope that allow acquiring brighter and sharper images when fluorescent observation is performed while stimulating a sample with light. Illumination light from a laser unit is split into stimulation light and excitation light by a dichroic mirror. In other words, half of the illumination light is transmitted through the dichroic mirror and becomes the stimulation light, and half of the illumination light is reflected by the dichroic mirror and becomes the excitation light. Half of the excitation light is reflected by a dichroic mirror and is irradiated onto a sample, and half of the stimulation light transmits through the dichroic mirror and is irradiated onto the sample. Fluorescence generated from the sample is totally reflected by the dichroic mirror and the dichroic mirror, and is received by a photodetector.

Abstract: A microscope system includes: an illumination unit; a light control direction unit specifying quantity of light output from the illumination unit; a storage unit storing voltage-related information in which a direction value specified by the light control direction unit and a voltage value to be applied to the illumination unit are associated with each other and are set, the direction value being in an entire range that can be specified by the light control direction unit under each observation condition, the voltage value corresponding to the direction value; a light quantity control unit controlling the quantity of the emitted light; and a control unit acquiring the voltage value corresponding to the specified direction value from the voltage-related information corresponding to the observation condition, and allowing the light quantity control unit to control the quantity of light based on the acquired voltage value.

Abstract: A microscope device according to the present disclosure (the present microscope device) includes: a light source configured to oscillate coherent illuminating light, the illuminating light being applied on a specimen; a detecting unit configured to detect fluorescent light from the specimen as feedback light, the specimen being irradiated with the illuminating light; a phase distribution control unit disposed in an optical path of the illuminating light, the phase distribution control unit being configured to control phase distribution of the illuminating light; a controller configured to control the phase distribution control unit to vary the phase distribution; and an image generating unit configured to operate a difference of the feedback light between before and after the phase distribution varies, to generate an image of the specimen.

Abstract: A microscope apparatus includes an illumination optical system that illuminates a sample with illumination light from a light source; an imaging optical system that converges light emitted from the sample to form a sample image by an objective lens; an aperture member disposed in the illumination optical system in the vicinity of a conjugate plane of a rear focal plane of the objective lens and having an aperture for limiting illumination light; and a filter member that includes a phase plate disposed in the imaging optical system in the vicinity of the objective lens rear focal plane or in the vicinity of the conjugate plane of the objective lens rear focal plane and having first and second phase areas introducing a 180-degree phase difference into the light from the sample; a phase boundary portion between the first and second phase areas being disposed in a conjugate aperture of the aperture.

Abstract: An apparatus and a method of locating a specimen with a microscope, the microscope including two point light sources, a transparent specimen holder, a digital imaging device, a processor, and a display. The method includes sequentially illuminating the two point light sources through the specimen holder, across the specimen, and onto the digital imaging device to create shadows associated with each of the two point light sources. Optical images of the specimen are received on the digital imaging device associated with each shadow from the light sources, and a disparity signal is output containing a horizontal, vertical, and depth position of the specimen relative to the digital imaging device. Location of the specimen is determined based on the disparity signal. The specimen is allowed to move freely throughout the transparent specimen holder.

Abstract: A microscope system includes a microscope body having a base portion forming a foundation, an arm portion extending substantially parallel to a bottom surface of the base portion, and a frame portion connecting ends of the base portion and the arm portion, having substantially a C shape in side view and holding an illumination optical system ejecting illumination light from a light source to a specimen. A light source unit is connected with the microscope body and radiates illumination light to the illumination optical system. A focusing unit supports a stage for placing the specimen and at least holding an objective lens focusing the specimen by collecting observation light from the specimen on the stage. The microscope body and the focusing unit do not contact each other in a state where an optical axis of the objective lens coincides with an optical axis of the illumination light.

Abstract: A measurement apparatus includes a lamp mount including a first mount and a second mount. The first mount has a first cavity to mount an observation module. The second mount has a second cavity to mount an image capture module. The measurement apparatus further includes a plurality of light modules mounted on an undersurface of the lamp mount. The second mount is disposed with an included angle relative to a first axis of the first cavity so that a second axis of the second cavity and the first axis converge on a point. The undersurface of the lamp mount is concave so that light from the light modules tilts toward the first axis, and the light and the first axis also converge on the point.

Abstract: A method for illuminating at least one sample in SPIM microscopy includes generating a light beam and forming a light strip from the light beam using an optical device that interacts with the light beam. The light strip is passed strip through at least one objective having optics configured to deliver detection light emanating from the sample directly or indirectly to a detector, with the objective optics interacting with the light strip. The light strip is deflected using a light-redirecting device downstream of the objective optics so as to propagate the light strip, after deflection, at an angle other than zero degrees with respect to an optical axis of the objective in order to illuminate the sample.

Abstract: A pinhole changing device for a confocal microscope is detailed herein. Several pinholes of different sizes are contained on a single disk. A precision XY stage is used to switch between the several pinholes. The same device could be used as a spatial filter for a multi-photon microscope. One can also add beam expansion control and spherical aberration correction to the same device with no additional loss.

Abstract: The present invention provides an apparatus including an aperture stop including a first aperture configured to define an illumination condition for illuminating an illumination surface to a first condition, and a second aperture configured to define the illumination condition to a second condition, and fixed on a pupil plane of an illumination optical system, a light shielding plate, and a driving unit configured to positions the light shielding plate such that a shielding region shields a second path extending from a light source to the illumination surface through the second aperture, when setting the illumination condition to the first condition, and positions the light shielding plate such that the shielding region shields a first path extending from the light source to the illumination surface through the first aperture, when setting the illumination condition to the second condition.

Abstract: The present invention discloses an optical system to generate incoherent structured illumination and an optical imaging system using incoherent structured illumination. The optical system includes: at least one coherent light source, a spatial light modulator, a plurality of optical lenses, a rotating diffuser for destroying the coherence of the structured illumination pattern, an objective, and a stage accommodating samples. The optical imaging system using incoherent structured illumination includes: an optical microscope having an objective and a beam splitter, a charge-coupled device camera for recording a sequence of images of the samples, a stage for accommodating and moving samples; a coherent light source; a spatial light modulator; a quarter wave plate, a plurality of optical lenses and mirrors; and a diffuser rotating 360 degrees or vibrating rapidly around the axis of the optical path continuously.

Abstract: An illumination apparatus includes a light source and a collimator optical system converting light emitted from the light source to approximately-parallel light. The collimator optical system includes, in an order of proximity to the light source, a first lens having a positive power and including an approximately-flat surface and an aspheric surface, and a second lens having a positive power.

Abstract: In accordance with the aspects of the present disclosure, a method and apparatus is disclosed for imaging interferometric microscopy (IIM), which can use an immersion medium to enhance resolution up to a resolution of linear systems resolution limit of ?/4n, where ? is the wavelength in free space and n is the index of refraction of a transmission medium.

Abstract: Illuminating arrangement for a microscope (200) having a first LED (10) for providing light with a first intensity spectrum (K1) with at least two intensity maxima and an intensity minimum located between the intensity maxima, and at least one further LED (20) for providing light with a further intensity spectrum (K2) respectively, each further intensity spectrum (K2) having an intensity maximum in the region of the intensity minimum of the first intensity spectrum (K1), and a device (30) for merging the light of the first LED (10) and the light of the at least one further LED (20), by means of which illuminating light can be produced with a combined intensity spectrum (K3) composed of light with the first intensity spectrum and light with each of the further intensity spectra.

Abstract: Provided is a scanning near-field optical microscope capable of obtaining, in a highly sensitive manner, optical information having a spatial frequency higher than a spatial frequency corresponding to a wavelength of irradiation light. A scanning near-field optical microscope 100 according to the present invention includes: a light irradiating part 102 for emitting illumination light toward a sample 107; a light receiving part 112 for receiving light; a microstructure for generating or selectively transmitting near-field light, the microstructure being disposed on at least one of an emission side of the light irradiating part 102 and an incident side of the light receiving part 112; and an ultrahigh-wavenumber transmitting medium 108 for transmitting near-field light, the ultrahigh-wavenumber transmitting medium exhibiting anisotropy in permittivity or permeability.

Abstract: In microscopes, particularly laser scanning microscopes, for detecting light coming from a sample, it is known to protect detectors from excessively high light outputs by means of shutters in the detection beam path. Further, in order to measure the light output impinging on the detector when the detection beam path is closed, a portion of the light is coupled out of the detection beam path and directed to a monitor diode. Constructions of this kind are complicated and costly. In the microscope according to the invention, a monitor diode is arranged on the shutter in such a way that the monitor diode is situated in the detection beam path when the shutter is closed. This makes it possible in a simple manner to measure the light output in a microscope when the detection beam path is closed even without additionally coupling light out of the detection beam path.

Abstract: Systems and methods are provided for illuminating a surface to be observed microscopically using a retractable beamsplitter. The retractable beamsplitter allows the use of coaxial illumination when the beamsplitter is positioned in the operator's line of sight. The retractable beamsplitter allows the use of non-coaxial illumination without reducing the amount of illumination that reaches the operator when the beamsplitter is retracted from the operator's line of sight. As a result a single system can be used effectively to provide various types of illumination.

Abstract: A method and system for three-dimensional polarization-based confocal microscopy are provided in the present disclosure for analyzing the surface profile of an object. In the present disclosure, a linear-polarizing structured light formed by an optical grating is projected on the object underlying profile measurement. By means of a set of polarizers and steps of shifting the structured light, a series of images with respect to the different image-acquired location associated with the object are obtained using confocal principle. Following this, a plurality of focus indexes respectively corresponding to a plurality of inspected pixels of each image are obtained for forming a focus curve with respect to the measuring depth and obtaining a peak value associated with each depth response curve. Finally, a depth location with respect to the peak value for each depth response curve is obtained for reconstructing the surface profile of the object.

Abstract: A motor-operated microscope system has a motor-operated microscope section including an illumination optical system, an electric stage, an image forming optical system having an objective lens and an image forming lens, and an image pickup device; a housing; a control device having a screen display and an arithmetic processing section; and software displaying an operating condition setting screen of the motor-operated microscope section on the screen display and controlling an operation of the motor-operated microscope section in accordance with a set condition.

Abstract: An arbitrary entire region of a specimen, or a plurality of individual regions, is simultaneously stimulated without a time lag, or a strong stimulus is applied to an arbitrary region of a specimen. The invention provides a microscope apparatus including a first stimulation optical system having a galvanometer mirror that scans a specimen with first stimulus light, which applies an optical stimulus to a specimen, on the specimen; a second stimulation optical system which has a plurality of two-dimensionally arrayed movable mirrors and which switches the angle of each movable mirror so that second stimulus light, which applies an optical stimulus to the specimen, can be selectively deflected towards the specimen; and a dichroic mirror that combines a light path of the first stimulation optical system and a light path of the second stimulation optical system.

Abstract: A microscope apparatus includes a microscope, a gooseneck holder, and an operating unit configured to control apparatus functions of the microscope apparatus. The operating unit is carried by the gooseneck holder.

Abstract: A microscope includes a light source including an LED device having a light radiating surface and a light directing element including a larger coupling-out surface. The light directing element is disposed so as to couple in light radiated by the light source and couple out the radiated light from the coupling-out surface. The light directing element is disposed so that the light is radiated out in an angular range of ±10° to ±50° and illuminates an area at 5 meters in an angular range of at least ±5° with intensity fluctuations of less than 50%. A condenser is disposed between the coupling-out surface of the light directing element and the object to be viewed. The condenser has an aperture with an aperture dimension and is disposed such that the aperture is irradiated completely with the light coupled out from the coupling-out surface.

Abstract: Fluorescence is generated from an irradiated point on an inspection surface of a sample and the fluorescence is collected by an objective lens. Here, because of the magnification chromatic aberration of the objective lens, the fluorescence going out from the objective lens travels along a path shifted from the irradiation light and changed substantially into a non-scan light by a galvano-scanner. The fluorescence passes through a dichroic mirror and comes into deflection system after light of unnecessary wavelength is removed by a filter. The deflection system is driven in synchronization with the galvano-scanner by a computer and corrects the shift and inclination of the optical axis generated by the magnification chromatic aberration of the objective lens. Then the fluorescence forms an image of the irradiation point of the inspection surface of the sample on a pin hole of a pin hole plate by using a collective lens.

Abstract: Instrument for obtaining wide field images of a specimen comprising (1) a divergence disperser, generating within a light beam different divergences in each of the chromatic components thereof to form images of the specimen at different depths thereof, and (2) a wavelength analyser, selecting the wavelength or interval of wavelengths of the light beam to form the image of the specimen at a specific depth. The light beam may be mono- or polychromatic and the instrument may have a configuration of illumination by transmission or by reflection. The instrument may moreover be modified through diverse configurations of the divergence disperser and/or of the wavelength analyser, including the motorisation thereof or the inclusion of a movable mask, together with being coupled to a CCD or CMOS camera to integrate the diverse images acquired from all sections of the specimen.

Abstract: System and method for selectively viewing features of objects, including features hidden under non-transparent materials.

Abstract: A total internal reflection fluorescence imaging apparatus according to an embodiment of the invention includes: a metal nanostructure layer, which includes a metal thin film and a nanostructure formed over the metal thin film; a light source unit, which provides incident light such that the incident light is totally reflected off the metal nanostructure layer and an evanescent wave localized in a horizontal direction is created between the metal nanostructure layer and a specimen arranged over the metal nanostructure layer; and a fluorescence image extracting unit, which extracts and images a fluorescence signal generated by the specimen due to the evanescent wave localized in a horizontal direction.

Abstract: It is an objection of the present invention to provide a fluorescence reading apparatus in view of the influence of fluorescence derived from a fluorescence substance that is not involved with an interaction between a probe substance and a target substance.

Abstract: The invention relates to a device for scanning an object comprising a focusing lens system (30) which focuses an illuminating light beam (24) onto a region of the object to be analyzed. An actuator assembly is coupled to the focusing lens system (30) and moves the focusing lens system (30) in accordance with a predefined scanning pattern transversely to the cecenternter axis of the illumination light beam (24) in a reference position of the illumination light beam (24). A front glass (38) is disposed downstream of the focusing lens system (30) viewed in the direction of the illuminating light beam (24). An internal immersion medium (40) is disposed between the focusing lens system (30) and the front glass (38). An external immersion medium (48) can be introduced between the front glass (38) and the object.

Abstract: A dark field microscope is capable of providing illumination with a long focal distance condenser lens having a low numerical aperture without the need for a diaphragm for an objective lens. A method is provided for effectively adjusting its optical axis. In particular embodiments, the dark field microscope includes; a light source for emitting illumination light; a light collecting optical system including a light collecting side condenser lens for collecting the illumination light from the light source to illuminate an observation sample; and an image forming optical system including an objective lens for receiving scattered light from the observation sample to form a magnified image of the sample, the light collecting side condenser lens is a long focal distance lens, and a light shielding member for shielding the illumination light is provided on a back focal plane or at an image forming center of it in the image forming optical system.

Abstract: The present invention relates to a method for illuminating a sample (10) in a microscope (26), the sample (10) being analysed in transmitted light bright field illumination or in incident light fluorescence illumination, wherein a white light LED (4) is used as the light source for the transmitted light bright field illumination, and a shutter (6) is activated at a location in the illumination beam path of the transmitted light bright field illumination during incident light fluorescence illumination and this shutter (6) is deactivated during transmitted light bright field illumination.

Abstract: A plurality of mounting devices are selectively exchanged while keeping the same parfocal distances for objective lenses among these mounting devices. A microscope system includes a microscope main unit and a plurality of attachable/detachable objective lens units that are selectively attached to the microscope main unit. The microscope main unit includes raising mechanism that can move the attached objective lens unit in an optical axis direction. The plurality of objective lens units have a revolver or a nosepiece that can be attached to the microscope main unit and an objective lens that can be mounted on the revolver or the nosepieces in an attachable/detachable manner. Distances from an attachment position in the microscope main unit for the revolver or the nosepiece to focal positions of the objective lenses are set to be mutually equal among the objective lens units.

Abstract: A particle image-capturing method includes controlling a light-emitting diode unit to emit light so as to illuminate a fluid through an annular condenser, particles in which scattering the light, and controlling an image-capturing unit to capture images of the scattered light through a microscope. A micro particle image velocimetry that performs the method is also disclosed.

Abstract: Among other items, an illumination device (110) is [provided] for an observation device (100) having one, two or more observation beam paths, each with at least one observation beam bundle, in particular for an operating microscope, having at least one light source (112) for producing at least one illumination beam path with at least one illumination beam bundle (121, 122, 123) for illuminating an object to be observed, in particular, an eye to be observed, in addition having at least one illumination optics unit (11)*, which is constructed according to the Köhler principle of illumination, as well as an objective element (119), wherein the at least one illumination beam path or the at least one illumination beam bundle (121, 122, 123) runs coaxially to an observation beam path or observation beam bundle.

Abstract: A multi-mode optical fiber delivers light from a radiation source to a multi-focal confocal microscope with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber.

Abstract: A device in the form of a scanning microscope, a device in the form of a structural unit for a microscope and a method and a device for optically scanning one or more samples. A device in the form of a scanning microscope has a light source (42), which emits an illuminating light beam (32). A focusing lens system (34) focuses the illuminating light beam (32) on a region to be examined of a sample (36). An actuator arrangement moves the focusing lens system (34) according to a prescribed scanning pattern transversely in relation to a center axis of the illuminating light beam (32) and/or in relation to a housing of a structural unit (20) that encloses the focusing lens system (34).

Abstract: An optical system is used in a microscope which is provided to observe a sample or in a light emitting device which applies a beam of light such as a laser beam onto a sample. The optical system can form an oblique path of light so that without shifting the sample or the position of the eyes of the observer (or the position of a light source of the light emitting device), the observer can observe (in the case of the light emitting device, it can apply light onto) not only a vertical top side the sample but also a front, rear, left or right side of the sample, or the position of a portion of the sample to be observed (in the case of the light emitting device, a portion onto which light is applied) can be varied.

Abstract: The automatic adjustment method of a microscopic image for automatically adjusting an image on the basis of the lightness of the microscopic image includes distinguishing an observation pixel being an observation target in the image from a non-observation pixel not being an observation target on the basis of the lightness of each pixel of the image, determining a representative value for representing the lightness of the image on the basis of the lightness of a selection pixel identified as the observation pixel and adjusting the lightness of the image on the basis of the representative value.

Abstract: The present invention relates to a microscope of which visibility, controllability and operability can be improved. In the microscope, an optical path and optical path of an image forming system are set so as to be perpendicular to each other when viewed from the top. In other words, in this microscope, there exists an ocular optical system that guides light, which propagates the optical path to optical path of the image forming system, to a user. The optical path is formed in a direction perpendicular to a direction of the light from a sample emitted from the ocular optical system to the user. The present invention can be applied to an inverted microscope.

Abstract: Systems and methods are provided for illuminating a surface to be observed microscopically using a retractable beamsplitter. The retractable beamsplitter allows the use of coaxial illumination when the beamsplitter is positioned in the operator's line of sight. The retractable beamsplitter allows the use of non-coaxial illumination without reducing the amount of illumination that reaches the operator when the beamsplitter is retracted from the operator's line of sight. As a result a single system can be used effectively to provide various types of illumination.

Abstract: A scanning microscope for manipulating a sample, the microscope, having a first light source, a second light source, a beam deflector, and an optical device. The first light source is configured to emit an illuminating light beam that follows an illuminating beam. A second light source is configured to produce a manipulating light beam which has a manipulating beam focus and follows a manipulating beam path. The beam deflection device is configured to guide the illuminating light beam and the manipulating beam focus over or through the sample. The optical device is disposed downstream of the second light in the manipulating beam and is configured to modify the size of the manipulating beam focus.

Abstract: A scanning microscope includes a light source, illumination optics, and a scanning device for moving the illumination focus across a target region and doing so by varying the direction of incidence in which the illuminating beam enters an entrance pupil of the illumination optics. To incline the illumination focus relative to the optical axis of the optics, the scanning device directs the illuminating beam onto a portion of the entrance pupil that is offset from the center of the pupil and, in order to move the illumination focus across the target region, the scanning device varies the direction of incidence of the illuminating beam within said portion. An observation objective is provided which is spatially separated from the illumination optics and disposed such that its optical axis (O3) is substantially perpendicular to the target region and at an acute angle (?) to the optical axis (O1) of the illumination optics.

Abstract: An improved head-mounted optical illuminator or magnifier of the type worn by a medical or dental professional includes an optical coating applied to one or more optical surfaces associated with the illuminator or magnifier, and wherein the optical coating is a rejection coating operative to blocks wavelengths in the green, blue, violet and/or ultraviolet portions of the electromagnetic spectrum, depending upon the embodiment. Short-wavelength coatings (blue/violet/uv) may be applied to the surface of a lens used in a head-worn illuminator, for example to the beam-forming optics. The head-worn illuminator may be an LED illuminator, xenon illuminator, or other high-intensity source. In the case of the green notch filter coatings, these would typically only be applied to a head-worn magnifier, including flip-up and through-the-lens styles. In all embodiments, the optical coating may be a multilayer dielectric coating, a holographic filter, or utilize other optical filter technology.

Abstract: An illumination optical system includes, in order from a light source side a collector lens, a field stop, a field lens having positive power, an aperture stop, and a collective lens having positive power. The illumination optical system is a substantially both-side telecentric optical system between the field stop and a sample surface, and satisfies the following conditional expressions where DFS indicates a diameter of the field stop, ? indicates a magnification from the sample surface to the field stop, and NA indicates a numerical aperture on the sample surface side of the illumination optical system. 15?DFS/??9??(1) 0.85?NA?0.

Abstract: A method and system for performing three-dimensional holographic microscopy of an optically trapped one dimensional structure. The method and system use an inverted optical microscope, a laser source which generates a trapping laser beam wherein the laser beam is focused by an objective lens into a plurality of optical traps. The method and system also use a collimated laser at an imaging wavelength to illuminate the structure created by the optical traps. Imaging light scattered by the optically tapped structure forms normalized intensity holograms that are imaged by a video camera and analyzed by optical formalisms to determine light field to reconstruct 3-D images for analysis and evaluation.

Abstract: Disclosed is an illumination system for an ophthalmic surgical microscope (10), including a light source (32) for emitting light along an illumination beam path (50) directed toward an eye (12) of a patient, and a measuring device (54, 62) for measuring a parameter of the patient's eye (12). The illumination system further includes an adjustable optical element (48) which allows the orientation of the illumination beam path (50) to be adjusted relative to an observation beam path (16L) of the surgical microscope (10) so as to obtain a red reflex, and a control device (60) which determines a red-reflex-optimized control parameter based on the measured parameter of the patient's eye (12) and adjusts the optical element (48) according to the red-reflex-optimized control parameter.

Abstract: The invention relates to an illumination device (1) for a microscope (10) utilizing at least one point light source (4) arranged on a carrier element (2), at least one carrier element (2) for receiving at least one point light source (4), and a holder (5) attachable to the microscope (10) and having an arc-shaped guide (6), being provided, the at least one carrier element (2) being mounted displaceably along the guide (6) in a horizontal plane. According to a further aspect, the illumination device (1) comprises a connector element (20) for attaching the illumination device (1) both to a stationary focusing column (12) and to an adjustable focusing arm (11) of a microscope (10).

Abstract: Provided is a microscope and an observation method which can improve spatial resolution. A microscope according to an aspect of the invention includes a laser light source (10), an objective lens (16) that focuses light from the laser light source on a sample, and a detector (22) that detects the laser light as signal light from a sample (17) when the sample (17) is irradiated with the laser light. The light is applied to the sample with an intensity changed to obtain a nonlinear region where intensities of the light and the signal light have a nonlinear relation due to occurrence of saturation or nonlinear increase of the signal light when the light has a maximum intensity, and the detector (22) detects the signal light according to the intensity of the laser light to perform observation based on a saturation component or a nonlinear increase component of the signal light.

Abstract: Systems and methods are provided for illuminating a surface to be observed microscopically using a retractable beamsplitter. The retractable beamsplitter allows the use of coaxial illumination when the beamsplitter is positioned in the operator's line of sight. The retractable beamsplitter allows the use of non-coaxial illumination without reducing the amount of illumination that reaches the operator when the beamsplitter is retracted from the operator's line of sight. As a result a single system can be used effectively to provide various types of illumination.

Abstract: An improved microscope stage mount with built-in fiduciary markers is used for fluorescence microscopy, and comprises: (a) an optically-transparent glass plate adapted for specimen mounting and microscope viewing and comprising a specimen mounting area; and (b) a defined and ordered, two-dimensional microscopic array of fiduciary markers, wherein the markers are polymeric pillars affixed to the plate about the specimen mounting area, wherein the markers provide a three-dimensional spatial reference for the specimen.

Abstract: A fluorescence observation system, a method for performing a fluorescence observation, and a set of filters that can be used in such system and method are provided.