Lyase (4. ) Patents (Class 435/232)
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Publication number: 20140323324Abstract: The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.Type: ApplicationFiled: December 16, 2011Publication date: October 30, 2014Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Hisashi Narimatsu, Jun Hirabayashi, Atsushi Kuno, Hideki Matsuzaki, Yuzuru Ikehara, Hiromi Ito, Yasuhiro Hashimoto, Keiro Shirotani, Satoshi Futakawa, Hajime Arai, Masakazu Miyajima, Kazuo Fujihara
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Patent number: 8870980Abstract: The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.Type: GrantFiled: December 21, 2011Date of Patent: October 28, 2014Assignee: Neste Oil OyjInventors: Annika Malm, Simo Laakso, Ossi Pastinen, Heidi Kahelin, Miia Mujunen
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Patent number: 8871485Abstract: Disclosed herein are modified carbonic anhydrase enzymes, and a process of using same for the extraction, production and purification of carbon dioxide gas. More particularly, modified carbonic anhydrase enzymes are used for the production, purification of carbon dioxide and the products of the hydration reaction, hydrogen and bicarbonate ions Also, this technology is used to enhance the production of carbon dioxide in blood or in reverse osmosis desalination to remove carbon dioxide. Specifically, the invention relates to a modified carbonic anhydrase enzyme possessing improved activity and a process whereby immobilized modified carbonic anhydrase contained within a reactor device catalyzes the reversible hydration of carbon dioxide.Type: GrantFiled: June 22, 2011Date of Patent: October 28, 2014Assignee: University of Florida Research Foundation, Inc.Inventors: Robert McKenna, David N. Silverman
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Publication number: 20140315275Abstract: The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.Type: ApplicationFiled: April 11, 2014Publication date: October 23, 2014Applicants: THE GOODYEAR TIRE & RUBBER COMPANY, DANISCO US INC.Inventors: Christopher L. RIFE, Derek H. WELLS
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Publication number: 20140314843Abstract: The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.Type: ApplicationFiled: April 17, 2014Publication date: October 23, 2014Applicant: Codexis, Inc.Inventors: Gjalt W. Huisman, Nicholas J. Agard, Benjamin Mijts, Jonathan Vroom, Xiyun Zhang
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Publication number: 20140315313Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.Type: ApplicationFiled: April 20, 2012Publication date: October 23, 2014Applicant: DANISCO US INC.Inventors: Elizabeth A. Bodie, Robert James Pratt, II
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Patent number: 8852583Abstract: One aspect of the present invention relates to mutants of chondroitinase ABCI. Such chondroitinase ABCI mutants exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including exposure to UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.Type: GrantFiled: July 6, 2012Date of Patent: October 7, 2014Assignee: Acorda Therapeutics, Inc.Inventors: Anthony O. Caggiano, Jennifer Iaci, Andrea Vecchione, Elizabeth Markensohn
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Publication number: 20140295493Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 1, 2014Publication date: October 2, 2014Inventors: Hiroaki Udagawa, Christian Isak Jorgensen
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Patent number: 8846366Abstract: The invention relates to R-hydroxynitrile lyases with improved substrate acceptance, increased activity and increased selectivity, obtainable by introducing random mutations with the aid of random mutagenesis and/or saturation mutagenesis techniques, identifying by means of screening or selection and, where appropriate, subsequently combining advantageous mutations, and to the use thereof.Type: GrantFiled: December 11, 2007Date of Patent: September 30, 2014Assignee: DSM IP Assets BVInventors: Richard Gaisberger, Anton Glieder, Zhibin Liu, Beate Pscheidt
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Publication number: 20140287419Abstract: Compositions and methods comprising polynucleotides and polypeptides having dicamba decarboxylase activity are provided. Further provided are nucleic acid constructs, host cells, plants, plant cells, explants, seeds and grain having the dicamba decarboxylase sequences. Various methods of employing the dicamba decarboxylase sequences are provided. Such methods include, for example, methods for decarboxylating an auxin-analog, method for producing an auxin-analog tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional dicamba decarboxylase variants.Type: ApplicationFiled: March 14, 2014Publication date: September 25, 2014Inventors: Eric Althoff, Yih-En Andrew Ban, Linda A. Castle, Daniela Grabs, Jian Lu, Phillip A. Patten, Yumin Tao, Alexandre Zanghellini
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Publication number: 20140273116Abstract: Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Applicant: Butamax Advanced Biofuels LLCInventors: Kristen J. Kelly, Rick W. Ye
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Publication number: 20140271603Abstract: A human cell line for implementing a method for preparing recombinant human factor H, more particularly the recombinant human factor H represented by the sequence SEQ ID NO: 1, or a variant possessing a percentage homology of at least 99% with the sequence SEQ ID NO: 1, with a yield greater than the quantity of endogenous factor H produced by said cell line.Type: ApplicationFiled: October 25, 2012Publication date: September 18, 2014Inventor: Toufik Abache
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Publication number: 20140256005Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: ApplicationFiled: May 29, 2014Publication date: September 11, 2014Applicant: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Patent number: 8828704Abstract: Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B12-independent butanediol dehydratases that catalyzes the substrate to product conversion of 2,3-butanediol to 2-butanone in the process of producing 2-butanol and 2-butanone.Type: GrantFiled: April 29, 2008Date of Patent: September 9, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Gail K. Donaldson, Andrew C. Eliot, Lori Ann Maggio-Hall, Vasantha Nagarajan, Charles E. Nakamura, Jean-Francois Tomb
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Patent number: 8821860Abstract: Targeted antimicrobials are described and related, compositions, methods and systems.Type: GrantFiled: August 6, 2010Date of Patent: September 2, 2014Assignee: Lawrence Livermore National Security, LLCInventors: Paul J. Jackson, Brian E. Souza, Feliza A. Bourguet, Matthew A. Coleman
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Publication number: 20140242036Abstract: The instant invention refers to a novel process for potentiating the production of substances with antifungal activity obtained from Ganoderma lucidum, using a technological device for the differential, qualitative and quantitative expression of proteins and other bioactive molecules, selected from the group consisting of polysaccharides, triterpenoids, fatty acids and ganoderic acids. The compositions containing said substances showed antifungal activity and mycelium growth and fungi ascospore germination inhibiting activity, amongst them Mycosphaerella fijiensis, primary pathogenic agent causing black Sigatoka disease in banana and plantain crop fields.Type: ApplicationFiled: June 18, 2013Publication date: August 28, 2014Inventors: JOHN JAIRO MIRA CASTILLO, PAOLA ANDREA ZAPATA OCAMPO, LUCíA ATEHORTÚA GARCÉS, LIUDA JOHANA SEPÚLVEDA ARANGO, DIEGO FERNANDO ROJAS VAHOS
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Publication number: 20140245496Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant AROM polypeptide and/or mutant catechol-O-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-D-glucoside.Type: ApplicationFiled: August 7, 2012Publication date: August 28, 2014Applicants: EVOLVA SA, INTERNATIONAL FLAVORS & FRAGRANCES INC.Inventors: Joergen Hansen, Esben Halkjaer Hansen, Honey Polur, Joseph M. Sheridan, Jonathan R. Heal, William D.O. Hamilton
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Publication number: 20140242668Abstract: The present invention relates to a variant of a mature polysaccharide lyase having a beta helix structure, wherein said variant comprises an N- and/or C-terminal truncation and consists essentially of at least 90% of the beta helix structure of the mature polysaccharide lyase.Type: ApplicationFiled: July 9, 2012Publication date: August 28, 2014Applicant: BIOMAY AGInventors: Michael Wallner, Michael Hauser, Gabriele Gadermaier, Martin Himly, Fatima Ferreira
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Patent number: 8815562Abstract: The present invention is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS) derived drug and analogs thereof. The invention also discloses polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS derived drug.Type: GrantFiled: April 15, 2011Date of Patent: August 26, 2014Assignees: The Regenys of the University of Michigan, Allegheny-Singer Research Institute, Colorado State University Research FoundationInventors: David H. Sherman, Garth D. Ehrlich, Benjamin Janto, Robert M. Williams, Christopher M. Rath
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Publication number: 20140234903Abstract: A novel gene cluster encoding polypeptides involved in the generation of bio synthetically unique peptide-based compounds is described. In addition, new methods for biosynthetic engineering and production of modified peptides and proteins are disclosed. Furthermore, new tools for identification of homologue polypeptides and precursor peptides in other species are provided. In addition, peptide-based compounds and fusions of these compounds with functional moieties for treatment of disorders such as tumors are disclosed.Type: ApplicationFiled: September 5, 2012Publication date: August 21, 2014Applicant: ETH ZURICHInventors: Jorn Piel, Cristian Gurgui, Michael Francis Freeman, Agustinus Robert Uria, Maximilian Johannes Helf
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Publication number: 20140220631Abstract: Disclosed are a method for rapid screening of suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.Type: ApplicationFiled: January 30, 2014Publication date: August 7, 2014Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Jung-Hoon SOHN, Eui-Sung CHOI, Jung-Hoon BAE, Eung-Suck LEE, Mi-Kyung SHIN
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Publication number: 20140220644Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: ApplicationFiled: June 7, 2012Publication date: August 7, 2014Applicant: Mitsubishi Rayon Co., LtdInventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara
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Patent number: 8796007Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.Type: GrantFiled: February 8, 2012Date of Patent: August 5, 2014Assignee: Chugai Seiyaku Kabushiki KaishaInventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
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Publication number: 20140206759Abstract: The invention relates to a method for producing biodegradable, functionalised polymer particles, and to the use of the same as medicament carriers.Type: ApplicationFiled: December 30, 2013Publication date: July 24, 2014Applicant: Polybatics LimitedInventor: Bernd Helmut Adam Rehm
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Publication number: 20140196166Abstract: An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.Type: ApplicationFiled: September 24, 2013Publication date: July 10, 2014Inventors: Katherine Zulak, Christopher Jones, Jessie Moniodis, Joerg Bohlmann
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Publication number: 20140193869Abstract: The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.Type: ApplicationFiled: December 20, 2013Publication date: July 10, 2014Applicant: GreenLight Biosciences, Inc.Inventors: William Jeremy Blake, James R. Swartz
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Patent number: 8771998Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.Type: GrantFiled: February 20, 2009Date of Patent: July 8, 2014Assignee: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20140186914Abstract: Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase.Type: ApplicationFiled: March 3, 2014Publication date: July 3, 2014Applicant: SCIENTIST OF FORTUNE, S.A.Inventor: Phillippe Marliere
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Publication number: 20140186910Abstract: Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.Type: ApplicationFiled: December 27, 2013Publication date: July 3, 2014Applicant: ButamaxTM Advanced Biofuels LLCInventors: LORI ANN MAGGIO-HALL, BRIAN JAMES PAUL, STEVEN CARY ROTHMAN, RICK W. YE
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Patent number: 8765442Abstract: The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.Type: GrantFiled: December 21, 2009Date of Patent: July 1, 2014Assignee: DuPont Nutrition Biosciences ApSInventor: Mette Zacho Grønfeldt
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Publication number: 20140178962Abstract: Engineered protein constructs with carbonic anhydrase catalytic activity, and their application in CO2 scrubbing.Type: ApplicationFiled: March 12, 2013Publication date: June 26, 2014Applicant: Imiplex LLCInventors: Francis Raymond Salemme, Patricia C. Weber
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Publication number: 20140178460Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: August 30, 2013Publication date: June 26, 2014Applicant: VERENIUM CORPORATIONInventors: David P. WEINER, Alexander VARVAK, Toby RICHARDSON, Mircea PODAR, Ellen BURKE, Shaun HEALEY
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Publication number: 20140157456Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.Type: ApplicationFiled: May 31, 2012Publication date: June 5, 2014Inventors: Robert Cornelis Schuurink, Micheal Abertus Haring, Petronella Martina Bleeker
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Publication number: 20140148502Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.Type: ApplicationFiled: February 3, 2014Publication date: May 29, 2014Applicant: MODERNA THERAPEUTICS, INC.Inventors: Stephane Bancel, Tirtha Chakraborty, Antonin de Fougerolles, Susan Whoriskey
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Patent number: 8735563Abstract: The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: GrantFiled: June 23, 2011Date of Patent: May 27, 2014Assignee: Novozymes A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa
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Patent number: 8735134Abstract: The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.Type: GrantFiled: October 26, 2012Date of Patent: May 27, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Christopher L. Rife, Derek H. Wells
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Patent number: 8735112Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.Type: GrantFiled: November 14, 2008Date of Patent: May 27, 2014Assignee: DSM IP Assets B.V.Inventors: René Verwaal, Liang Wu, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt
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Publication number: 20140140977Abstract: The present invention relates to methods and kits for determining heparanase procoagulant activity in a biological sample. The invention also relates to diagnostic methods for the detection and/or monitoring of a coagulation-related pathologic disorder in a mammalian subject. The invention further relates to compositions comprising heparanase and at least one tissue factor, uses and methods based thereon in the treatment, amelioration and prevention of coagulation-related pathologic conditions. The invention still further relates to methods for screening a coagulation modulatory compound, methods and uses based thereon in the treatment, amelioration and prevention of coagulation-related pathologic conditions.Type: ApplicationFiled: January 18, 2012Publication date: May 22, 2014Inventors: Yona Nadir, Benjamin Brenner, Israel Vlodavsky
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Publication number: 20140141473Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.Type: ApplicationFiled: April 20, 2012Publication date: May 22, 2014Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve
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Publication number: 20140140978Abstract: The present invention relates to methods and pharmaceutical compositions for treating cancer. More specifically, the invention relates to a polypeptide isolated from Brevibacterium aurantiacum that shows methionine gamma-lyase and homocysteinase activities. The present invention also relates to the use of such a polypeptide for the treatment of cancer.Type: ApplicationFiled: June 15, 2012Publication date: May 22, 2014Applicants: INSERM (Institut National de la Sante et de la Recherche Medicale), UNIVERSITE PARIS SUD-PARIS XI, INRA (Institut National de la Recherche Agronomique)Inventors: David Machover, Pascal Bonnarme
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Patent number: 8728791Abstract: The present invention provides a microorganism which possesses the formate dehydrogenase gene and hydrogenase gene and contains an exogenous transcription activator gene for formate hydrogen lyase system, characterized in that said microorganism shows the transcription activator for formate hydrogen lyase system highly expressed therein and shows an improved function to generate hydrogen from formic acid, and a process for producing hydrogen using the microorganism. Utilization of the microorganism of the present invention enables the hydrogen production from an organic substrate to be accomplished on a practical, commercial scale. The hydrogen to be produced by the present invention, which is free of carbon monoxide being causative to poisoning of the electrode catalyst for fuel cells, is useful as a fuel for fuel cells.Type: GrantFiled: December 7, 2005Date of Patent: May 20, 2014Assignees: Research Institute of Innovative Technology for the Earth, Sharp CorporationInventors: Hideaki Yukawa, Masayuki Inui, Akihito Yoshida, Naoto Torata
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Patent number: 8728792Abstract: The present invention provides a modified ethylenediamine-N,N?-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N?-disuccinate:ethylenediamine lyase activity.Type: GrantFiled: January 21, 2010Date of Patent: May 20, 2014Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Takanori Akiyama, Wataru Mizunashi, Tetsuji Nakamura
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Publication number: 20140127251Abstract: The present invention provides new immunological compositions and vaccines comprising selected M. tuberculosis antigens and antigenic peptides as well as nucleic acids encoding said antigens for use in the prevention, prophylaxis and treatment of mycobacterial infection, especially tuberculosis. In particular the invention provides recombinant BCG based vaccines in which one or more of the selected M. tuberculosis antigens are over expressed. The invention further provides isolated peptides for use in methods for diagnosing, characterizing, or classifying mycobacterial infections.Type: ApplicationFiled: April 11, 2012Publication date: May 8, 2014Applicant: ALARUM DEVELOPMENT LTD.Inventor: Markus Maeurer
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Publication number: 20140120588Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.Type: ApplicationFiled: May 30, 2012Publication date: May 1, 2014Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Fumiaki Watanabe, Takanori Ambo, Ai Hara
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Patent number: 8709407Abstract: Methods and composition related to the engineering of a novel protein with methionine-?-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-?-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.Type: GrantFiled: February 3, 2011Date of Patent: April 29, 2014Assignee: AEMase, Inc.Inventors: George Georgiou, Everett Stone
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Publication number: 20140113344Abstract: The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c): (a) a polynucleotide comprising (i) the nucleotide sequence represented by SEQ ID NO:1, or (ii) the nucleotide sequence consisting of the nucleotide residues at positions 133 to 1785 in the nucleotide sequence represented by SEQ ID NO:1; (b) a polynucleotide that comprises a nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; or (c) a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; and the like.Type: ApplicationFiled: March 15, 2013Publication date: April 24, 2014Applicants: AJINOMOTO CO., INC., Bridgestone CorporationInventors: YASUYUKI HAYASHI, MINAKO HARADA, SAAYA TAKAOKA, YASUO FUKUSHIMA, KEIICHI YOKOYAMA, YOSUKE NISHIO, YOSHINORI TAJIMA, YOKO MIHARA, KUNIO NAKATA
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Publication number: 20140113339Abstract: The present invention relates to a technique for capturing carbon dioxide and converting the carbon dioxide to carbonate minerals using a recombinant whole cell biocatalyst expressing carbonic anhydrase. More particularly, the present invention relates to a composition for capturing carbon dioxide and a method for capturing carbon dioxide using the composition, which composition comprises a whole cell of a transformant formed with a vector including a nucleic acid encoding a recombinant carbonic anhydrase; a cell lysate or its fraction of the whole cell; or a recombinant carbonic anhydrase isolated from the whole cell. Further, the present invention relates to a composition and method for converting the carbon dioxide to carbonate minerals using the carbon dioxide capturing composition.Type: ApplicationFiled: April 13, 2012Publication date: April 24, 2014Inventors: Hyung Joon Cha, Im Gyu Kim, Dong Gyun Kang, Byung Hoon Jo, Jeong Hyun Seo
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Publication number: 20140113346Abstract: The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide.Type: ApplicationFiled: May 8, 2012Publication date: April 24, 2014Applicant: DANISCO US INC.Inventors: Jing Ge, Ling Hua, Ayrookaran J. Poulose
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Publication number: 20140113338Abstract: The invention relates to an alpha-ketopimelic acid decarboxylase enzyme that is a homologue of SEQ ID NO:2, comprising at least one mutation selected from a group of substitutions listed in the specification, to a method for preparing 5-formyl valeric acid (hereinafter also referred to as ‘5-FVA’), to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’), to a method for preparing ?-caprolactam (hereinafter referred to as ‘caprolactam’) from 6-ACA, to a method for the preparation of adipic acid, to a method for preparing diaminohexane. The invention further relates to a host cell which may be used in a method according to the invention and to a polynucleotide encoding an alpha-ketopimelic acid decarboxylase enzyme.Type: ApplicationFiled: March 30, 2012Publication date: April 24, 2014Applicant: DSM IP ASSETS B.V.Inventors: Axel Christoph Trefzer, Stefanus Cornelis Hendrikus Josef Turk, Jan Metske Van Der Laan, Iise De Lange, Denise Ilse Jacobs
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Patent number: 8703466Abstract: An object of the present invention is to provide a DOI synthase having properties such as stability to heat and pH, which are superior to those of conventional enzymes, and a method for producing DOI using the above-mentioned enzyme. The present invention provides a 2-deoxy-scyllo-inosose synthase having the properties described in the following (1), (2), (4), (6) and (7), and also having the properties described in the following (3) and/or (5): (1) action: the enzyme has a function to convert glucose-6-phosphate to 2-deoxy-scyllo-inosose; (2) optimum pH range: pH 7.0 to 7.7; (3) stable pH range: pH 6.0 to 8.0; (4) optimum temperature range: 55° C. to 70° C.; (5) stable temperature range: 20° C. to 46° C.; (6) coenzyme used: NAD+; and (7) molecular weight: 39,000 to 42,000.Type: GrantFiled: March 26, 2010Date of Patent: April 22, 2014Assignee: Asahi Kasei Chemicals CorporationInventors: Kazunobu Konishi, Shinichi Imazu