Containing Chemically Combined Protein Or Biologically Active Polypeptide Patents (Class 525/54.1)
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Patent number: 8647613Abstract: The present invention has an object of providing a drug carrier capable of controlling in vivo pharmacokinetics. The present invention is directed to a drug carrier comprising a molecular assembly having a drug incorporated therein, and the above object can be achieved by a part of the amphiphilic molecules included in the molecular assembly being released from the molecular assembly by an external environmental change. The present invention utilizes a phenomenon that the hydrophilic-hydrophobic balance of the amphiphilic molecules is shifted toward hydrophilicity by an external environmental change and thus the amphiphilic molecules are freed from the molecular assembly.Type: GrantFiled: March 10, 2006Date of Patent: February 11, 2014Assignees: JCR Pharmaceuticals Co., Ltd., Waseda UniversityInventors: Shinji Takeoka, Yosuke Okamura, Hideo Kanazawa, Shuji Hisamoto, Kohei Kubota, Yosuke Obata
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Publication number: 20140039125Abstract: A drug delivery system for delivering a drug payload to a specific tissue or cell type is disclosed. The system includes a polymalic acid molecular scaffold which can be used for attaching a plurality of molecular modules. Molecular modules include targeting antibodies for promoting cellular uptake by a target cell, and pro-drugs for altering cellular metabolism, for example, a pro-drug that alters expression of protein kinase CK2.Type: ApplicationFiled: September 19, 2013Publication date: February 6, 2014Applicants: Arrogene Nanotechnology, Inc., Cedars-Sinai Medical CenterInventors: Julia Y. Ljubimova, Keith L. Black, Eggehard Holler
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Publication number: 20140037588Abstract: Provided herein are compositions, devices and systems comprising thermoresponsive, biodegradable elastomeric materials, and methods of use and manufacture thereof.Type: ApplicationFiled: January 31, 2012Publication date: February 6, 2014Applicant: NORTHWESTERN UNIVERSITYInventors: Jian Yang, Robert Van Lith, Guillermo Ameer
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Patent number: 8642309Abstract: The invention relates to an amphiphilic polymer comprising at least 75% of amphiphilic monomers of formula (I), the average molar mass of the polymer being between 800 and 100 000, and also water-soluble complexes between the polymer according to the invention and a hydrophobic or amphiphilic compound, in particular a membrane protein, a concentrated aqueous solution of one or more such complex(es), a product comprising one or more such complex(es) attached to a substrate by means of the polymer according to the invention, and various uses of these products.Type: GrantFiled: November 16, 2010Date of Patent: February 4, 2014Assignees: Centre National de la Recherche Scientifique (CNRS), University d'Avignon et des Pays de VaucluseInventors: Bernard Pucci, Fabrice Giusti, Paola Bazzacco, Jean-Luc Popot, Sharma Kshatrapati Shivaji, Gregory Durand
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Publication number: 20140030210Abstract: The present disclosure relates to a new polymerisation process in which ethylenically unsaturated monomers are polymerised by a living radical polymerisation process in the presence of an initiator and a catalyst. Polymers produced by this new process are also thought to be novel and may be used to derivatise biological molecules to improve their efficacy as therapeutic treatments. A preferred polymer is of formula The polymers are particularly suitable for derivatising proteins, such as interferon-?.Type: ApplicationFiled: September 26, 2013Publication date: January 30, 2014Applicant: BIOCOMPATIBLES UK LIMITEDInventors: Stephen James BROCCHINI, Antony Robert GODWIN, Yiqing TANG, Andrew Lennard LEWIS
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Publication number: 20140024722Abstract: Described herein are block copolymer conjugates that form double-network hydrogels under appropriate conditions. The conjugates comprise a block of polymer end-group, a block of self-associating peptide or protein, and flexible linkers between the two. Hydrogels comprising the conjugates have the mechanical properties, including elastic modulus and fracture toughness, required for load-bearing applications, while maintaining desirable shear-thinning properties, for example, for injectability.Type: ApplicationFiled: October 5, 2011Publication date: January 23, 2014Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGYInventors: Bradley D. Olsen, Matthew J. Glassman, Jacqueline Chan
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Publication number: 20140024776Abstract: The present invention provides multi-armed high MW polymers containing hydrophilic groups and one or more functional agents, and methods of preparing such polymers.Type: ApplicationFiled: May 23, 2013Publication date: January 23, 2014Applicant: OligasisInventors: Stephen A. Charles, Victor D. Perlroth, Didier G. Benoit, Lane A. Clizbe, Wayne To, Linda J. Zadik, Jeanne M. Pratt
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Patent number: 8633277Abstract: Methods for preparing polymer-drug conjugates are provided. The disclosed methods involve polymeric reagents comprising a moiety of atoms arranged in a specific order, wherein the moiety is positioned between a water-soluble polymer and a reactive group. Related methods, compositions, preparations, and so forth are also provided.Type: GrantFiled: March 8, 2011Date of Patent: January 21, 2014Assignee: Nektar TherapeuticsInventors: J. Milton Harris, Antoni Kozlowski, Samuel P. McManus, Michael D. Bentley, Stephen A. Charles
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Publication number: 20140017328Abstract: A composition of matter for use in encapsulating a drug is expressed by formula (1) or formula (2): wherein R1 and R2 are each independently a hydrogen atom or a substituted or unsubstituted, linear or branched alkyl group having 1 to 12 carbon atoms; A is a hydrophilic polymer chain; L1 and L3 are each a linking group; B is a cation-containing group; R3 is a side chain of an amino acid; z is an integer of 5 to 500; x is an integer of 40% or more of z; y is 0 or a positive integer; z-x-y is 0 or a positive integer; p is an integer of 1 to 10; and q is an integer of 1 to 10.Type: ApplicationFiled: January 16, 2012Publication date: January 16, 2014Applicants: NANOCARRIER CO., LTD., THE UNIVERSITY OF TOKYOInventors: Kazunori Kataoka, Ronald James Christie, Nobuhiro Nishiyama, Kanjiro Miyata, Shigeto Fukushima, Yu Matsumoto, Takahiro Nomoto, Yasuki Kato
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Publication number: 20140017676Abstract: Collection devices and kits for biological sample collection include a biologic sample collection device having a hydrophilic swab matrix that includes a modified polycaprolactone (PCL). Methods of production and use thereof are also described herein. The biologic sample collection devices, kits and methods described herein are used to collect a biologic sample (e.g., blood, buccal cells, etc.) and to enable extraction of nucleic acids (e.g., DNA) from that biologic sample so that the nucleic acids can be analyzed (e.g., sequencing and subsequent analyses of DNA).Type: ApplicationFiled: July 13, 2012Publication date: January 16, 2014Applicant: DIOMICS CORPORATIONInventor: Deirdre Morhet
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Publication number: 20140017169Abstract: The caged cell-penetrating peptide (cCPP) conjugates of this invention are ideal for intracellular delivery of a broad variety of cargoes including various nanoparticulate pharmaceutical carriers (liposomes, micelles, microparticles, nanoparticles, polymer-conjugates). The conjugates comprise a detectable agent or a therapeutic agent, and the conjugates provide a novel strategy for site-specific delivery of the same to appropriate tissues in the subject. Versatile application of the conjugates in diagnostics and imaging is described.Type: ApplicationFiled: May 26, 2011Publication date: January 16, 2014Inventors: Ayelet David, Gonen Ashkenasy, Yossi Shamay
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Publication number: 20140017265Abstract: A terminally modified polymer is provided herein. At least one terminus of the polymer is —O—(CH2)2-LM or —O—CH2—CH(OH)—CH2—CR1?CR2R3. LM, R1, R2, and R3 are defined herein Also disclosed are terminal conjugates comprising the polymer and a pharmaceutically useful modifier, as well as compositions comprising the conjugates, methods of their preparation, and methods of treating various disorders with the conjugates or their compositions.Type: ApplicationFiled: July 3, 2013Publication date: January 16, 2014Inventors: Aleksandr V. Yurkovetskiy, Venu R. Gurijala, Joshua D. Thomas, Timothy B. Lowinger
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Publication number: 20140017762Abstract: Cross-linked, conjugated organic semiconducting polymer networks that combine improved solubility with improved electrical and/or optical properties in one package have been developed. New materials that combine advantages of good charge-carrier mobility organic materials and conjugated polymer networks as well as fairly good solubility in common organic solvents, into one package and thus offers a general and powerful platform suitable for use in numerous applications.Type: ApplicationFiled: March 28, 2012Publication date: January 16, 2014Applicant: Hitachi Chemical Research Center, Inc.Inventor: Cuihua Xue
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Publication number: 20140011760Abstract: Methods for forming, modifying, and drug loading of a copolymer are provided, along with the resulting products. The method can include polymerizing 2-(pyridin-2-yldisulfanyl)ethyl acrylate with poly(ethylene glycol)methacrylate via free radical polymerization to form the copolymer. The molar ratio of 2-(pyridin-2-yldisulfanyl)ethyl acrylate to poly(ethylene glycol)methacrylate is about 100:1 to about 1:100. After polymerizing, the copolymer can be reacted with a thiol monomer that contains a carbon-bonded sulfhydryl to modify end groups on a first portion of the 2-(pyridin-2-yldisulfanyl)ethyl acrylate repeating units. Thereafter, the copolymer can be crosslinked, and a drug can then be loaded into the crosslinked copolymer.Type: ApplicationFiled: June 28, 2013Publication date: January 9, 2014Inventors: Peisheng Xu, Remant Bahadur K.C.
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Publication number: 20140004048Abstract: The invention relates to water-soluble nanoparticles and methods for making such nanoparticles. Specifically, the invention relates to dendrimerization to enhance the solubility of nanoparticles.Type: ApplicationFiled: June 19, 2013Publication date: January 2, 2014Inventors: Sergei A. VINOGRADOV, Tatiana V. Esipova
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Publication number: 20140004076Abstract: This invention relates to a recombinant human G-CSF (rhG-CSF) dimer and its use in the treatment of neurological disorder. In particular, upon ischemic neural injury in animal, this invention can be used to protect neurons with the use of rhG-CSF dimer such that function of injured nerves can be restored. Serum half-life of G-CSF dimer of this invention is prolonged and the biological activity thereof is increased.Type: ApplicationFiled: August 30, 2013Publication date: January 2, 2014Applicant: GENERON (SHANGHAI) CORPORATION LTD.Inventors: Xiaoqiang YAN, Zhihua HUANG, Hongzhou YANG, Bill N.C. SUN, Yuliang HUANG
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Publication number: 20130344027Abstract: Multifunctional polymers are disclosed having a smart segment and a biodegradable segment. Advantageously, the biodegradable segment includes a hydrophilic segment and a hydrophobic segment. Embodiments include combining the multifunctional polymeric material with a biologically active substance in an aqueous loading environment and administering the composition as a drug delivery vehicle to a human subject.Type: ApplicationFiled: August 27, 2013Publication date: December 26, 2013Applicant: University of Tennessee Research FoundationInventors: Tao Lu Lowe, Young Shin Kim, Xiao Huang
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Publication number: 20130344592Abstract: Factor H-binding peptides that binds to a region of factor H that does not impede the complement-inhibitory activity of factor H are disclosed. When immobilized onto the surface of a biomaterial, these peptides recruit factor H, resulting in a substantial inhibition of biomaterial-induced complement activation in a biological substance exposed to the biomaterial.Type: ApplicationFiled: December 19, 2011Publication date: December 26, 2013Inventor: John D. Lambris
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Patent number: 8614291Abstract: The present invention is directed to absorbable polyesters comprising one or more monophosphate functionality; a conjugate comprising the foregoing polyester and a peptide and/or a bioactive agent; microparticles comprising an absorbable polyester; a conjugate comprising the microparticles and a peptide and/or a bioactive agent; an acylated or alkylated polysaccharide having one or more monophosphate functionality; a conjugate comprising the acylated or alkylated polysaccharide and a peptide and/or a bioactive agent; and pharmaceutical compositions thereof.Type: GrantFiled: January 27, 2006Date of Patent: December 24, 2013Assignee: Poly-Med, Inc.Inventors: Shalaby Wahba Shalaby, Joel Thomas Corbett
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Publication number: 20130337534Abstract: The present invention relates to polymeric reagents and conjugates thereof, methods for synthesizing the polymeric reagents and conjugates, pharmaceutical compositions comprising the conjugates and methods of using the polymer conjugates including therapeutic methods where conjugates are administered to patients.Type: ApplicationFiled: August 5, 2013Publication date: December 19, 2013Applicant: Oligasis, LLCInventor: Stephen A. Charles
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Patent number: 8604127Abstract: The present invention relates to a conjugate of a protein or peptide with a polyethylene glycol derivative having catechol, wherein the protein or peptide is mono-PEGylated at the N-terminal with the polyethylene glycol derivative, and to a preparation method thereof. According to the invention, the catechol-PEG derivative can be site-specifically conjugated with the N-terminal amine group of a protein or peptide, so that a homogeneous polyethylene glycol-protein or -peptide conjugate can be obtained in high yield. Unlike a prior art conjugate, the conjugate obtained according to the invention allows the decrease in the activity of the protein to be minimized without chemically modifying the protein, and thus the conjugate has an excellent pharmacological effect. Also, because the conjugate is homogeneous, the process for preparing the conjugate can be simplified. Moreover, the conjugate has uniform biological efficacy in vivo and shows strong resistance to hydrolysis and thus a long in vivo duration time.Type: GrantFiled: October 29, 2010Date of Patent: December 10, 2013Assignees: Korea Advanced Institute of Science and Technology, Inno Therapy Inc.Inventors: Haeshin Lee, Hyukjin Lee, In Taek Song, Moon Sue Lee
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Patent number: 8604159Abstract: The present invention provides, among other things, methods for preparing functionalized and other polymers from polymer from polymer alcohols such as poly(ethylene glycol)s. In addition, polymer compositions, conjugates, polymeric reagents, are also provided.Type: GrantFiled: July 22, 2004Date of Patent: December 10, 2013Assignee: Nektar TherapeuticsInventors: Samuel P. McManus, Antoni Kozlowski, J. Milton Harris
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Patent number: 8598269Abstract: The invention is based on the recognition that known antimicrobial compounds, such as nisin or other lantibiotics, can be made to form a long lasting antimicrobial surface coating by linking the peptide with a block polymer, such as PLURONIC® F108 or an end group activated polymer (EGAP) in a manner to form a flexible tether and/or entrap the peptide. The entrapped peptide provides antimicrobial action by early release from entrapment while the tethered peptide provides longer lasting antimicrobial protection. Antimicrobial gels and foams may be prepared using the antimicrobial peptide containing block copolymers.Type: GrantFiled: May 15, 2012Date of Patent: December 3, 2013Assignees: Allvivo Vascular, Inc., The State of Oregon Acting by & through the State Board of Higher Ed. on Behalf of Oregon State Univ.Inventors: Jennifer A. Neff, Joseph McGuire, Pranav R. Joshi
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Publication number: 20130312961Abstract: Biomimetic adhesive compositions can be used in various aspects of subterranean treatment operations. Methods for treating a subterranean formation can comprise: providing an adhesive composition that comprises a first polymer comprising a plurality of monomers that comprise a phenolic moiety, a biopolymer that is crosslinkable with the first polymer, a crosslinking agent, and an oxidizing agent; introducing the adhesive composition into a subterranean formation; and forming a coacervate-bound surface in the subterranean formation by crosslinking the first polymer.Type: ApplicationFiled: May 23, 2012Publication date: November 28, 2013Applicant: Halliburton Energy Services, Inc.Inventors: Enrique A. Reyes, Pinmanee Boontheung, Narongsak Tonmukayakul
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Publication number: 20130317172Abstract: A method for manufacturing a carrier having temperature responsive protein A, which is protein A mutated such that a binding property to an antibody changes depending upon temperature, immobilized thereto, wherein the temperature responsive protein A has the binding property to the antibody in a first temperature region and no binding property to the antibody in a second temperature region; and the method comprises a step of immobilizing the temperature responsive protein A to a carrier surface at a temperature within the first temperature region in which the temperature responsive protein A has the binding property to the antibody.Type: ApplicationFiled: December 26, 2011Publication date: November 28, 2013Applicants: NOMADIC BIOSCIENCE CO., LTD., ASAHI KASEI MEDICAL CO., LTD.Inventors: Ichiro Koguma, Hiroki Shigematsu, Kazuo Okuyama, Satoshi Sato
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Patent number: 8591951Abstract: Biocompatible non-toxic triblock copolymers having an A-B-A structure wherein each A is a hydrophilic, biocompatible end block and the B middle block is a hydrophobic desaminotyrosyl tyrosine polycarbonate or polyarylate. The copolymers spontaneously self-assemble to form low critical aggregation concentration nanospheres having utility as delivery vehicles for hydrophobic biologically or pharmaceutically active compounds.Type: GrantFiled: July 27, 2005Date of Patent: November 26, 2013Inventors: Joachim B. Kohn, David Devore, Larisa Sheihet, Robert Dubin
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Publication number: 20130309192Abstract: Auristatin compounds and conjugates thereof are provided herein. The conjugate comprises a protein based recognition-molecule (PBRM) and a polymeric carrier substituted with one or more -LD-D, the protein based recognition-molecule being connected to the polymeric carrier by LP. Each occurrence of D is independently an Auristatin compound. LD and LP are linkers connecting the therapeutic agent and PBRM to the polymeric carrier respectively. Also disclosed are polymeric scaffolds useful for conjugating with a PBRM to form a polymer-drug-PBRM conjugate described herein, compositions comprising the conjugates, methods of their preparation, and methods of treating various disorders with the conjugates or their compositions.Type: ApplicationFiled: July 17, 2013Publication date: November 21, 2013Applicant: MERSANA THERAPEUTICS, INC.Inventors: Aleksandr V. Yurkovetskiy, Mao Yin, Timothy B. Lowinger, Joshua D. Thomas, Charles E. Hammond, Cheri A. Stevenson, Natalya D. Bodyak, Patrick R. Conlon, Dmitry R. Gumerov
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Patent number: 8586671Abstract: Biocompatible phase invertible proteinaceous compositions and methods for making and using the same are provided. Phase invertible compositions in accordance with the invention are prepared by combining a liquid proteinaceous substrate and a liquid crosslinking composition, where the liquid crosslinking composition includes a macromolecular crosslinking agent. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.Type: GrantFiled: May 15, 2012Date of Patent: November 19, 2013Assignee: Tenaxis Medical, Inc.Inventors: Ronald Dieck, Ian J. Handley, Neil Winterbottom, Joanna Wang
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Patent number: 8586020Abstract: Provided are a use of chemically-crosslinkable, poly(organophosphazene)s for biomaterials, chemically-crosslinkable poly(organophosphazene)s with a physiologically active substance covalently-bonded thereto, a use thereof for biomaterials, and a process for preparing the same. The chemical crosslinkings can be made by UV irradiation, and/or a crosslinker, and/or an additive, and/or an enzyme, and/or a mixing of at least one polymer.Type: GrantFiled: June 30, 2011Date of Patent: November 19, 2013Assignee: Korea Institute of Science and TechnologyInventors: Soo-Chang Song, Thrimoorthy Potta, Mi-Ran Park
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Publication number: 20130295040Abstract: The application provides a method of producing a comb polymer comprising the steps of: (a) Providing: (i) a plurality of monomers which are linear, branched or star-shaped, substituted or non-substituted, and have an olefinically unsaturated moiety, the olefinically unsaturated moiety being capable of undergoing addition polymerisation; (ii) an initiator compound; the initiator compound comprising a homolytically cleavable bond. (iii) a catalyst capable of catalysing the polymerisation of the monomer; and (b) Causing the catalyst to catalyse, in combination with the initiator, the polymerisation of a plurality of the monomers to produce the comb polymer. Catalysts and polymers obtainable by the process are also provided. Preferably, the comb polymer is capable of binding proteins and may be produced from monomers which are alkoxy polyethers, such as poly(alkyleneglycol) or polytetrahydrofuran.Type: ApplicationFiled: April 5, 2013Publication date: November 7, 2013Inventor: Warwick Effect Polymers Limited
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Patent number: 8575282Abstract: A modified compound that has at least one further functional group, in particular a bio- or macromolecule, comprising at least one x-fold (x?1) chemoselectively incorporated phosphoramidate group of general formula (I), NPO(OR1)(OR1?), and/or at least one x-fold (x?1) chemoselectively incorporated phosphonamide group of general formula (Ia), NPO(R1)(OR1?). R1 and R1? is selected from the group containing glycerol, polyglycerol, PEG polymers of the general empirical formula C2nH4n+2On+1 with n?1, Cn-alkyl chains with n?1; functionalized Cn-alkyl chains with n?1, aryls, heteroaryl, silyl, lipids, fluorophores, saccharides, peptides, crown ethers, or a linker, which links the aforementioned groups. R1 and R1? can be identical to or different from one another.Type: GrantFiled: March 9, 2010Date of Patent: November 5, 2013Assignee: Freie Universitaet BerlinInventors: Christian Hackenberger, Giuseppe Del Signore, Remigiusz Serwa, Ina Wilkening, Robert Vallee
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Publication number: 20130287726Abstract: A method of selecting a semaphorin for treating cancer in a subject is disclosed. The method comprises determining an expression of a semaphorin receptor on tumor cells of a tumor sample of the subject wherein an amount of the semaphorin receptor is indicative of the semaphorin suitable for treating the cancer in the subject. Methods of treating angiogenesis, kits for treating cancer and pharmaceutical compositions comprising semaphorins are also disclosed.Type: ApplicationFiled: July 15, 2013Publication date: October 31, 2013Inventors: Gera NEUFELD, Boaz Kigel, Ofra Kessler, Asya Varshavsky
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Publication number: 20130281623Abstract: The present invention relates to a water-soluble polymer complex that includes a water-soluble block copolymer and a magnetic nanoparticle, wherein the water-soluble polymer complex has a nonzero net magnetic moment in the absence of an applied magnetic field at ambient temperatures. The water-soluble block copolymer is preferably a diblock or triblock copolymer and the magnetic nanoparticle is preferably a ferrimagnetic or ferromagnetic nanoparticle. The water-soluble complexes may be derivatized with reactive groups and conjugated to biomolecules. Exemplary water-soluble polymer complexes covered under the scope of the invention include PEG112-b-PAA40 modified CoFe2O4; NH2-PEG112-b-PAA40 modified CoFe2O4; PNIPAM68-b-PAA28 modified CoFe2O4; and mPEG-b-PCL-b-PAA modified CoFe2O4.Type: ApplicationFiled: April 23, 2012Publication date: October 24, 2013Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Krishna M. Bajjuri, David Berman, Qiu Dai, Pierre-Olivier Jubert, Hareem T. Maune, Alshakim Nelson
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Publication number: 20130280782Abstract: The present disclosure provides protein-containing compositions, methods and uses thereof. A conjugate comprises a globular protein conjugated with a polymer that preserves the folded and functional structure of a protein. In some embodiments, a fusion protein comprises a globular protein conjugated with an elastin-mimic polymer (EMP). Also disclosed is an assembled solid-state or gel-state nanostructure comprising a plurality of conjugates, wherein each comprises a globular protein conjugated with a polymer that preserving the folded and functional structure of a protein. In certain embodiments, provided compositions and methods further comprise an additive.Type: ApplicationFiled: October 26, 2011Publication date: October 24, 2013Applicant: Massachusetts Institute of TechnologyInventors: Bradley D. Olsen, Carla S. Thomas
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Publication number: 20130281624Abstract: The present disclosure describes environmentally responsive polypeptides capable of displaying stimuli-triggered conformational changes in a reversible or irreversible manner that may be accompanied by aggregation. Polypeptides include a number of repeated motifs and may be elastomeric or non-elastomeric. The polypeptides may be used to deliver therapeutics to a biological site and to develop bioactive polypeptides that are environmentally responsive.Type: ApplicationFiled: May 29, 2013Publication date: October 24, 2013Inventors: Ashutosh Chilkoti, Felipe Garcia Quiroz, Miriam Amiram
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Patent number: 8562964Abstract: A structured drug system that is useful for delivering a drug payload to a specific tissue or cell type is disclosed. The system is based on purified polymalic acid. This polymer isolated from natural sources is biocompatible, biodegradable and of very low toxicity. The polymer is extremely water soluble and contains a large number of free carboxyl groups which can used to attach a number of different active molecules. In the examples disclosed N-hydroxysuccinimide esters of the carboxyl groups are used to attach such molecules. The active molecules include monoclonal antibodies to promote specific cellular uptake and specific pro-drugs such as antisense nucleic acids designed to modify the cellular metabolism of a target cell. The pro-drugs are advantageously linked by a somewhat labile bond so that they will be released under specific conditions. In addition, the system contains amide-linked valine to encourage membrane disruption under lysosomal conditions.Type: GrantFiled: April 29, 2011Date of Patent: October 22, 2013Assignees: Cedars-Sinai Medical Center, Arrogene Nanotechnology, Inc.Inventors: Julia Y. Ljubimova, Keith Black, Eggehard Holler
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Patent number: 8557182Abstract: This invention provides molecularly imprinted polymers (MIPs) for the detection of analytes, methods for forming the MIPs and detecting the analyte using the MIPs. The MIP comprises templated sites which are formed using a mimic of the analyte such that a reporter compound can be selectively attached at the templated sites, thus providing a site selectively tagged and templated MIP.Type: GrantFiled: December 8, 2004Date of Patent: October 15, 2013Assignee: The Research Foundation of State University of New YorkInventor: Frank V. Bright
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Publication number: 20130261259Abstract: This invention pertains to methods for producing homogeneous recombinant proteins that contain polymer initiators at defined sites. The unnatural amino acid, 4-(2?-bromoisobutyramido)phenylalanine of formula 1, was designed and synthesized as a molecule comprising a functional group further comprising an initiator for an atom-transfer radical polymerization (‘ATRP”) that additionally would provide a stable linkage between the protein and growing polymer. We evolved a Methanococcus jannaschii (Mj) tyrosyl-tRNA synthetase/tRNACUA pair to genetically encode this unnatural amino acid in response to an amber codon. To demonstrate the utility of this functional amino acid, we produced Green Fluorescent Protein with the unnatural amino acid initiator of formula 1 site-specifically incorporated on its surface (GFP-1).Type: ApplicationFiled: March 7, 2013Publication date: October 3, 2013Applicant: FRANKLIN AND MARSHALL COLLEGEInventors: Ryan A. Mehl, Krzysztof Matyjaszewski, Saadyah Averick
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Patent number: 8545830Abstract: Multifunctional polymers are disclosed having a smart segment and a biodegradable segment. Advantageously, the biodegradable segment includes a hydrophilic segment and a hydrophobic segment. Embodiments include combining the multifunctional polymeric material with a biologically active substance in an aqueous loading environment and administering the composition as a drug delivery vehicle to a human subject.Type: GrantFiled: March 24, 2004Date of Patent: October 1, 2013Assignee: University of Tennessee Research FoundationInventors: Tao Lu Lowe, Young Shin Kim, Xiao Huang
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Publication number: 20130245199Abstract: The present invention is directed to a functionalized elastomer comprising: a polymeric backbone chain derived from a monomer comprising at least one conjugated diene monomer and optionally at least one vinyl aromatic monomer; and a functional group bonded to the backbone chain, the functional group comprising an oligopeptide or modified oligopeptide.Type: ApplicationFiled: March 14, 2012Publication date: September 19, 2013Inventors: Ralf Mruk, Robert Fokko Roskamp, Alexandra Hermann, Rudolf Wilhelm Zentel
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Publication number: 20130244301Abstract: The present invention provides a thermoresponsive nanoparticle useful for the stabilization of enzymes in environments having a temperature greater than thirty degrees Centigrade. The thermoresponsive nanoparticle has (a) a functionalized enzyme conjugate having one or more enzymes or biological catalysts, the enzymes or biological catalysts are modified with palmitic acid N-hydroxysuccinimide ester and acryclic acid N-hydroxysuccinimide ester, and (b) a thermally responsive polymer, wherein the functionalized enzyme conjugate is encapsulated within the thermally responsive polymer. A nanocatalyst is provided that has one or more proteins. The proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell. The proteins are one or more enzymes or biological catalysts. A method for protecting the proteins is also set forth.Type: ApplicationFiled: May 7, 2013Publication date: September 19, 2013Inventors: Jeremy P. Walker, Anna M. Leech
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Publication number: 20130245198Abstract: The present invention is directed to a pneumatic tire comprising at least one component, the at least one component comprising a rubber composition comprising: a functionalized elastomer comprising: a polymeric backbone chain derived from a monomer comprising at least one conjugated diene monomer and optionally at least one vinyl aromatic monomer; and a functional group bonded to the backbone chain, the functional group comprising an oligopeptide or modified oligopeptide.Type: ApplicationFiled: March 14, 2012Publication date: September 19, 2013Inventors: Ralf Mruk, Robert Fokko Roskamp
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Publication number: 20130243862Abstract: A water-soluble photo-activatable polymer including: a photo-activatable group adapted to be activated by an irradiation source and to form a covalent bond between the water-soluble photo-activatable polymer and a matrix having at least one carbon; a reactive group adapted to covalently react with a biomaterial for subsequent delivery of the biomaterial to a cell; a hydrophilic group; and a polymer precursor. A composition including a monomolecular layer of the water-soluble photo-activatable polymer and a matrix having at least one carbon, wherein the monomolecular layer is covalently attached to the matrix by a covalent bond between the photo-activatable group and the at least one carbon. The composition further includes a biomaterial having a plurality of active groups, wherein the biomaterial is covalently attached to the monomolecular layer by covalent bonding between the active groups and reactive groups. Also provided is a method for delivery of a biomaterial to a cell.Type: ApplicationFiled: September 7, 2012Publication date: September 19, 2013Applicant: The Children's Hospital of PhiladelphiaInventors: Ivan Alferiev, Ilia Fishbein, Michael Chorny, Robert J. Levy, Benjamin Yellen, Darryl Williams
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Patent number: 8535879Abstract: The present invention relates to a method for the preparation of a surface structured bulk hydrogel bearing a topographic and chemical pattern, comprising the steps of: (i) providing a primary master having a predetermined topographic pattern, (ii) replicating said primary master by an elastomeric secondary master to obtain a secondary master with said predetermined topographic pattern in its inverse form, (iii) replicating said secondary master by an imprintable curable material of hydrogel precursor molecules which are capable of forming a hydrogel once crosslinked by curing, and crosslinking said material to thereby yield a replica made of cross-linked bulk hydrogel bearing the topographic pattern identical to that of the primary master, and (iv) functionalizing, particularly biofunctionalizing, the bulk hydrogel replica.Type: GrantFiled: May 23, 2007Date of Patent: September 17, 2013Assignee: DWI an der RWTH Aachen e.V.Inventors: Marga Lensen, Martin Möller
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Publication number: 20130234067Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.Type: ApplicationFiled: April 18, 2013Publication date: September 12, 2013Inventors: Daniel T. Chiu, Changfeng Wu, Xuanjun Zhang, Jiangbo Yu, Fangmao Ye
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Patent number: 8530212Abstract: A process for producing a substrate having an adhesive surface, which process comprises: (a) contacting the substrate with a carbene precursor, which carbene precursor is a compound of the following formula (1): whose substituent groups are defined herein, provided that when R is aryl or heteroaryl, said aryl or heteroaryl may be substituted by one, two, three, four or five groups, which groups are independently selected from various groups including -LB-WB; and (b) either: (i) when WA or WB comprises an adhesive functional group, generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, thereby yielding said substrate having an adhesive surface; or (ii) when WA or WB comprises a group which is a precursor of an adhesive functional group, generating a carbene reactive intermediate from the carbene precursor so that it reacts with the substrate to functionalise the surface, and (c) converting said group which is a precursor into anType: GrantFiled: August 22, 2007Date of Patent: September 10, 2013Assignee: ISIS Innovation LimitedInventors: Mark Moloney, Jon-Paul Griffiths
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Publication number: 20130231023Abstract: Binder compositions are described, where the compositions include a protein, a first crosslinking compound that includes a carbohydrate, and a second crosslinking compound that includes two or more primary amine groups. The first and second crosslinking compounds may be individually crosslinkable with each other and with the protein. Also described are fiber products that may include inorganic or organic fibers and a cured thermoset binder prepared from a protein and at least two crosslinking compounds. Additionally, methods of making fiber products are described that include providing inorganic or organic fibers, and applying a liquid binder composition to the fibers to form a fiber-binder amalgam. The liquid binder composition may include a protein and at least two crosslinking compounds that include a carbohydrate and an organic amine with two or more primary amines. The amalgam may be heated to a curing temperature to form the fiber product.Type: ApplicationFiled: March 2, 2012Publication date: September 5, 2013Inventors: Mingfu Zhang, Jawed Asrar, Uranchimeg Lester
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Patent number: 8524830Abstract: A resin composition includes a polyester capable of forming a crystal structure, and a substance represented by the following general structural formula (1), the substance represented by the following general structural formula (1) has a dehydration-condensed structure of two molecules of natural product-derived ?-amino acids or a substitution structure thereof, and the two molecules of ?-amino acids are not simultaneously glycine. In the above general structural formula, R1, R2, R3, and R4 indicate groups or substituent groups thereof bonded to the ? carbons of the ?-amino acids.Type: GrantFiled: October 1, 2009Date of Patent: September 3, 2013Assignee: Sony CorporationInventors: Noritaka Sato, Tsutomu Noguchi
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Publication number: 20130224825Abstract: Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.Type: ApplicationFiled: March 21, 2013Publication date: August 29, 2013Applicant: 3M INNOVATIVE PROPERTIES COMPANYInventor: 3M INNOVATIVE PROPERTIES COMPANY
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Patent number: 8519051Abstract: The present invention provides a block copolymer for a drug conjugate which comprises a water-soluble polymer region consisting of polyethylene glycol and a polyamino acid region having a hydrazide group and a hydrophobic group in the side chain.Type: GrantFiled: October 19, 2007Date of Patent: August 27, 2013Assignee: Nanocarrier Co., Ltd.Inventors: IuIian Bobe, Naoya Shibata, Hiroyuki Saito, Mitsunori Harada