Abstract: A method for generating a series of digital responses to the presence of a given concentration of analyte in a test sample is described. The responses are obtained by providing varying levels of components of a competing reaction with constant levels of components of a control reaction. The pattern of digital responses permits a direct reading of the level of analyte in the test sample. Analytes may be either substrates for enzyme reactions, or may be, themselves, enzymes.

Abstract: A method for the production of substrates with a uniform dispersion of extremely fine granules comprising: forming extremely fine granules in a granule-formation chamber under a reduced pressure by a gas-evaporation technique, introducing said extremely fine granules into a granule-recovering chamber under high vacuum, which is adjacent to said granule-formation chamber, through a slit formed in the partition between said granule-formation chamber and said granule-recovering chamber, and allowing said extremely fine granules to be dispersed on and attached to a supporter disposed in said granule-recovering chamber.

Abstract: A recombinant procaryotic microorganism containing the gene coding for insulin.

Abstract: An improved receptacle and spike assembly for athletic shoes is described. The spike assembly contains four fastening holes, and supports a spike and a connector. The improved receptacle is secured to a plate by embedding injectable material into cavities surrounding a suitable connector.

Abstract: A platinum resistor for the measurement of temperatures having a silicon substrate, an aluminum oxide film formed on said silicon substrate, and a platinum film formed on said aluminum oxide film.

Abstract: A moldable bone-implant material containing between about 65%-95% hard filler particles and a binder composed of between about 35%-5% of a biocompatible, biodegradable, thermoplastic polymer which has fluidic flow properties at or below about 60.degree. C. One preferred binder is polylactic acid having a molecular weight between about 400 and 5,000 daltons, and a preferred hard filler is hydroxylapatite. In use, the material is warmed to a temperature which allows molding, and the bone site is filled with the moldable material, which then forms a contour-fitting, semi-rigid implant. The implant retains its contour fit and acquires a rigid final state as the binder in the implant is gradually biodegraded and replaced through tissue ingrowth from the surrounding bone site.

Abstract: An injectable collagen material composed of reconstituted, mechanically sheared atelopeptide collagen fibers. The material is prepared by passing reconstituted collagen fibers repeatedly through a rigid mesh screen, until a substantial reduction in fiber size and size-heterogeneity is achieved. The mechanically sheared fibers may be further crosslinked to improve implant characteristics.

Abstract: A polymer having an isothianaphthene structure represented by the formula (Ia) and/or Ib): ##STR1## wherein R.sup.1 and R.sup.2 each represents a hydrogen atom or a hydrocarbon residue having 1 to 5 carbon atoms such as methyl, methoxy and thiomethyl, with the proviso that R.sup.1 and R.sup.2 may link together to form, along with the benzene ring, a fused ring which is naphthalene; X is sulfur, selenium or tellurium; Y.sup.- represents an anion of an electrolyte; z represents a number from 0.01 to 1 showing a ratio of the anion per mol of a monomer; and n represents a number of from 5 to 500 showing a degree of polymerization.

Abstract: An enhanced agglutination assay method for determination of a multivalent analyte is disclosed. Analyte is added to agglutinatable particles coated with anti-analyte molecules to produce particle agglutination. The extent of agglutination is enhanced by mixing the particles and analyte with an analyte-binding reagent composed of lipid bodies. The reagent bodies act by promoting multiple analyte bridge connections between individual bridged particles and a reagent body. Also disclosed is a kit containing such particles and reagent.

Abstract: Leukocytes are radioimmunoimaged by injecting patients with an immunoreactive nonleukocidal conjugate of an anti-leukocyte antibody and a gamma-emitting radioactive metal chelate, waiting for the conjugate to localize on the leukocytes, injecting the patient with an antibody to the conjugate to clear the blood of background nonlocalized conjugate, and visualizing the leukocytes by scintillation scanning.

Abstract: By careful screening and mutation, a human-murine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. The trioma fusion products of this immortalizing partner are stable producers of human monoclonal antibodies. A trioma which produces monoclonal human anti-varicella zoster is disclosed.

Abstract: Recombinant vaccines for immunizing horses against equine influenza virus (EIV) are disclosed. The DNA sequences encoding the hemagluttinin (HA) and neuraminidase (NA) glycoproteins from the two strains of EIV currently infective in horses are used to construct vaccinia carried vaccines, to design synthetic peptides for primer and booster administration, and to permit recombinant synthesis of HA and/or NA protein based vaccines. These DNA sequences also provide probes useful for preparing similar vaccines from fresh isolates of new strains generated by genetic drift.

Abstract: An improved integrated injection logic structure utilizes a current mirror in conjunction with each switching transistor (M.sub.1, M.sub.2) of the integrated injection logic circuit of this invention by connecting one of a plurality of collectors (O.sub.0, P.sub.0) of the switching transistor to the base of said switching transistor. In this manner, the current flowing through conducting switching transistors is limited by the current mirror. This limited current flow through conducting switching transistors, as well as the use of voltage pull up means (D.sub.1, D.sub.2) connected to the collectors of the switching transistors prevents the saturation of conducting switching transistors.

Abstract: A partially purified proteinaceous bone-inducing factor of 10,000 to 30,000 daltons is described. It is derived from demineralized bovine bone by extraction with a chaotropic agent, gel filtration, cation exchange chromatography using carboxymethyl cellulose at pH 4.8 and gradient elution with NaCl at 10 mM to about 150 mM.

Abstract: A process for transmitting a stream of digital data over a high impedance metallic pair transmission line under high loss conditions is disclosed. This process involves first converting the digital data into a stream of binary code. The first element of the code has a bit cell voltage-time wave shape that begins at 0 volts, moves sharply to an initial voltage A different than zero, at mid cell traverses 0 volts to a second voltage B of similar magnitude but opposite sign to the initial voltage A and that returns to zero at the end of the bit cell. The wave shape has a duty cycle of 80% or greater. The second element of said code is a mirror image of the first element. This code is applied to one end of the transmission line. the stream of binary code is then detected at the other end of the transmission line and reformatted to digital data.

Abstract: The present invention utilizes the power available for application to a static RAM cell in a manner which provides efficient use of the power so that greater standby power may be applied to the static RAM to increase the memory speed. The current required to maintain the memory cell in a preset state flows from the U.sub.cc source through a row of parallel memory cells and through a common bias supply and various peripheral circuits, such as decoders. A shunt voltage regulator controls the dependence of the common bias supply voltage on current fluctuations caused by addressing and deaddressing the memory cells. The invention includes an isolation device for isolating a particular row of memory cells when it is addressed without disturbing the bias on other memory cell rows. Similarly, the reference voltages of each of the peripheral circuits can be made independent of the common bias supply voltage and independent of the other peripheral circuits by the use of a local voltage regulator on each peripheral.

Abstract: This invention provides a live attenuated human rotavirus vaccine and a method for the preparation of such a vaccine. The method overcomes problems experienced hitherto in the growth of human rotaviruses, by the use of larger quantities of inocula at the initial growth stage and subsequent passages.

Abstract: A simple method for making EDTA, ED3A or DTPA analogs from amide derivatives of alpha amino acids is disclosed. These EDTA, ED3A or DTPA analogs are useful chelating agents, and preferably are useful as bifunctional chelating agents which may be attached to biological molecules and which form physiologically stable chelates with a variety of metal ions.

Abstract: A liposome assay reagent for determination of an analyte in a homogeneous immunoassay. The reagent includes a suspension of oligolamellar lipid vesicles containing encapsulated glucose-6-phosphate dehydrogenase (G6PD), at a specific activity of between about 1-10 units/.mu.mole vesicle lipid, and glucose-6-phosphate (G6P) at a concentration of at least about 5 mM. The encapsulated G6P protects the enzyme against inactivation on preparation, by reverse phase evaporation in the presence of organic solvent, and on storage as an aqueous suspension.

Abstract: Vaccines comprising peptide sequences corresponding to immunorecessive determinants in gonorrhea pilus protein are disclosed. The vaccines are effective in protecting human subjects against infection by a wide range of gonorrhea strains by raising antibodies which interfere with the colonization of the epithelium by the infecting bacteria or which enhance phagocytosis.