Abstract: Methods and composition are provided for diagnostic assays, where a sample traverses a bibulous matrix in a first plane and the flow path redirected into a second plane at a binding site, where a signal is created in relation to the presence of analyte. Various protocols may be employed where components of a signal producing system may be combined with the sample, the matrix, or added at the binding site.

Abstract: A DNA probe has been isolated which is capable of hybridizing to an oligonucleotide sequence coding for a polypeptide from a major 64 Kilodalton protein of human cytomegalovirus (HCMVgp64). The probe has a sequence of at least seventeen (17) to as many as seven hundred twenty-one 721) nucleotides. The probe may be labelled as by radioactivity. The probe has been used to screen DNA fragments constituting a subgenomic library of human cytomegalovirus DNA to obtain DNA fragments coding for the major late protein of human cytomegalovirus. The DNA fragments coding for the major late protein of human cytomegalovirus (HCMVgp64) may be hybridized to DNA fragments of HCMV DNA from an individual having human cytomegalovirus infection. The viral DNA can be used as whole HCMV DNA or as fragments formed by digesting the human cytomegalovirus DNA with a restriction endonuclease such as one of the restriction endonucleases EcoRI, BamHI, XbaI, HindIII and PrtI.

Abstract: A chimeric anti CEA antibody comparable to ATCC Accession No. BH 8747 is described.

Abstract: Stable alkaline aqueous solutions of sodium tetraphenylborate.

Abstract: Methodology is described for the carboxyl-terminal sequencing of proteins and peptides using novel coupling reagents.

Abstract: A method and apparatus for sequencing polypeptides, including a continuous flow reactor which may include a sample bearing membrane strip.

Abstract: Reagents useful for the C-terminal sequencing of proteins and peptides are disclosed. The reagents include sodium trimethylsilanolate and trimethyl N-oxide. The reagents are used as cleaving reagents in a method for the sequential C-terminal degradation of peptides and proteins.

Abstract: A DNA probe has been isolated which is capable of hybridizing to an oligonucleotide sequence coding for a polypeptide from a major 64 Kilodalton protein of human cytomegalovirus (HCMVgp64). The probe has a sequence of at least seventeen (17) to as many as seven hundred twenty-one (721) nucleotides. The probe may be labelled as by radioactivity. The probe has been used to screen DNA fragments constituting a subgenomic library of human cytomegalovirus DNA to obtain DNA fragments coding for the major late protein of human cytomegalovirus. The DNA fragments coding for the major late protein of human cytomegalovirus (HCMVgp64) may be hybridized to DNA fragments of HCMV DNA from an individual having human cytomegalovirus infection. The viral DNA can be used as whole HCMV DNA or as fragments formed by digesting the human cytomegalovirus DNA with a restriction endonuclease such as one of the restriction endonucleases EcoRI, BamHI, XbaI, HindIII and PrtI.

Abstract: A thorteveitite ore beneficiation process which comprises comminuting the ore by wet autogenuous grinding to substantially liberate the thortveitite contained therein, and passing the comminuted ore through a nonuniform magnetic field to produce a concentrate and a tailing, the concentrate containing a substantially greater percentage of thortveitite than the ore.

Abstract: A method of correcting spinal deformities and a spinal fixation system for use in the method. After surgically exposing the spine posteriorly, a series of clamps are affixed to the laminae of the vertebrae. Beginning at either end of the line of clamps, a rod attachment device is attached to each affixed clamp. Again, beginning at either end of the line, a rod is inserted into the rod attachment devices. Compression or distraction is then applied to the spine between successive vertebrae proceeding from one clamp to the next until all clamps are secured to the rod.

Abstract: A process for producing a slide bearing a spaced array of specimen fragments which comprises (i) cutting at least one specimen into a plurality of narrow strips; (ii) separating the plurality into groups of specimen strips; (iii) separately positioning strips from the groups in parallel grooves in a mold; (iv) embedding the strips in the mold in a first embedding medium to provide a structure comprising a base member having opposed first and second surfaces, the first surface being substantially planar; the second surface having ridges containing a specimen strip extending therefrom; (v) forming a stack of elements, each element corresponding to the structure, with the terminal surface of the ridges of an upper structure abutting the substantially planar first surface of the next lower structure; the spaces between the ridges defining channels for receipt of a fluid; (vi) embedding the stack in a second embedding medium to form a block having a spaced array of parallel specimen strips embedded therein; the st

Abstract: A method of forming an eroded pattern on a metal part using Electrical Discharge Machining (EDM) comprising: forming a pattern of electrodes on the end of a piece of electrode material by using wire EDM, the pattern of electrodes being the pattern to be eroded on the metal part or being a portion of the pattern; and mounting the metal part for EDM with the pattern of electrodes being mounted for use as electrodes and the erosion of the pattern or portion of the pattern provided by the pattern of electrodes by EDM in the metal part.

Abstract: In accordance with the present invention, a breathing exercise training apparatus is provided for increasing the endurance of inspiratory muscles. This is accomplished by setting a target breathing frequency, target inhalation time and target inhalation pressure on a target generator, and then having the trainee breathe into a resistive breathing device that imposes a predetermined resistance to inhalation and communicates actual inhalation pressure to the target generator. The target generator includes structure for comparing the actual inhalation time and pressure with the target values and provides a feedback signal in response thereto. A preset time interval is provided within which the trainee must attain the target inhalation pressure after an inhalation cycle begins, or a "fault" signal is given to the trainee. Thus, the apparatus of the invention forces the trainee to exercise his inspiratory muscles in order to achieve the target inhalation pressure within the target time.

Abstract: A method of activating human insulin receptor kinase and a method of reactivating human receptor kinase which has lost some, but not all, of its kinase activity, comprising reacting human insulin receptor with at least one basic protein.

Abstract: A process which comprises administering an antagonist of protein kinase C and/or the insulin receptor tyrosine kinase to a mammal in an amount therapeutically effective to inhibit lipogenesis in the mammal.

Abstract: An apparatus for collecting blood or other body fluid from a donor, in which a length of tubing has a first needle on one end for insertion into the vein of a donor, and a frangible joint between the ends of the tubing to separate the length of tubing into first and second parts. The frangible joint includes a combination needle shield and test tube receptacle on one end of the first part, disposed coaxially to a second needle used to fill test tubes with material drawn through the first needle. The combination shield and test tube receptacle is adapted to receive a test tube therein for operative relationship with the second needle so that the test tube may be filled with the material. A second shield is slidable on the tubing and has a retracted position adjacent the frangible joint and an operative position extended over the first needle when that needle is withdrawn from the vein.

Abstract: An apparatus for cooling melt spun filament bundles by controlled positioning of walls or air stream limiting wings in the quench chamber.

Abstract: A method of forming an eroded pattern on a metal part using Electrical Discharge Machining (EDM) comprising: forming a pattern of electrodes on the end of a piece of electrode material by using wire EDM, the pattern of electrodes being the pattern to be eroded on the metal part or being a portion of the pattern; and mounting the metal part for EDM with the pattern of electrodes being mounted for use as electrodes and the erosion of the pattern or portion of the pattern provided by the pattern of electrodes by EDM in the metal part.

Abstract: A multi-panel shield or sheath constructed to define an envelope into which an intravenous needle having a sharpened cannula may be withdrawn upon removal from a vein. The envelope has a compression flap adapted to overlie the venipuncture site while the needle is being withdrawn from the vein, whereby a nurse, doctor or other person using the device may exert pressure on the venipuncture site as the needle is being withdrawn. A gripping panel is also provided to facilitate holding of the sheath as the needle is being retracted into the envelope. After the needle has been withdrawn into the shield envelope, the panels may be folded over one another to completely enclose the needle for subsequent handling and/or disposal. In some embodiments of the inventions a non-folding sheath defining an envelope is utilized. Other embodiments have no compression panel.

Abstract: Methodology involving novel coupling reagents for the carboxyl-terminal amino acid sequence analysis of peptides and proteins is described.