Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having cellobiose dehydrogenase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
Type:
Grant
Filed:
January 5, 2000
Date of Patent:
August 28, 2001
Assignee:
Novozymes Biotech, Inc.
Inventors:
Elizabeth J. Golightly, Kimberly M. Brown
Abstract: The present invention relates to isolated polypeptides having galactose oxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
February 24, 2000
Date of Patent:
August 21, 2001
Assignee:
Novo Nordisk Biotech, Inc.
Inventors:
Elizabeth Golightly, Randy M. Berka, Michael W. Rey
Abstract: The present invention relates to methods of producing hemoproteins comprising (a) introducing into a filamentous fungal cell, which is capable of producing the hemoprotein, (i) one or more first control sequences capable of directing the expression of a heme biosynthetic enzyme encoded by a first nucleic acid sequence endogenous to the filamentous fungal cell, wherein the one or more of the first control sequences are operably linked to the first nucleic acid sequence; and/or (ii) one or more copies of one or more second nucleic acid sequences encoding a heme biosynthetic enzyme; (b) cultivating the filamentous fungal cell in a nutrient medium suitable for production of the hemoprotein and the heme biosynthetic enzymes; and (c) recovering the hemoprotein from the nutrient medium of the filamentous fungal cell.
Type:
Grant
Filed:
July 18, 2000
Date of Patent:
July 17, 2001
Assignee:
Novozymes Biotech, Inc.
Inventors:
Susan L. Elrod, Joel R. Cherry, Aubrey Jones
Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.
Type:
Grant
Filed:
February 26, 1999
Date of Patent:
July 3, 2001
Assignee:
Novozymes Biotech, Inc.
Inventors:
William Widner, Alan Sloma, Michael D. Thomas
Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having L-amino acid oxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
Abstract: The present invention relates to polypeptides having laccase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides.
Type:
Grant
Filed:
October 28, 1998
Date of Patent:
June 5, 2001
Assignee:
Novozymes Biotech, Inc.
Inventors:
Debbie Sue Yaver, Kimberley M. Brown, Sakari Kauppinen, Torben Halkier
Abstract: The present invention relates to methods for preparing a dough, including incorporating into the dough an effective amount of one or more pectate lyases which improve one or more properties of the dough or a baked product obtained from the dough. The present invention also relates to methods for preparing a baked product. The present invention also relates to compositions including an effective amount of one or more pectate lyases for improving one or more properties of a dough and/or a baked product obtained from the dough. The present invention further relates to doughs or baked products and to pre-mixes for a dough.
Abstract: The present invention relates to isolated polypeptides having phytase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to composite feeds and methods of reducing phytate levels.
Type:
Grant
Filed:
September 30, 1998
Date of Patent:
April 24, 2001
Assignee:
Novo Nordisk Biotech, Inc.
Inventors:
Randy M. Berka, Michael W. Rey, Alan V. Klotz
Abstract: The present invention relates to isolated polypeptides having 5-aminolevulinic acid synthase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Abstract: The present invention relates to polypeptides having laccase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides.
Type:
Grant
Filed:
September 2, 1999
Date of Patent:
March 27, 2001
Assignee:
Novo Nordisk of Biotech, Inc.
Inventors:
Debbie Sue Yaver, Kimberley M. Brown, Sakari Kauppinen, Torben Halkier
Abstract: The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
Type:
Grant
Filed:
October 3, 1997
Date of Patent:
February 13, 2001
Assignees:
Novo Nordisk Biotech, Inc., Novo Nordisk A/S
Inventors:
Alexander Blinkovsky, Randy Berka, Michael Rey, Elizabeth Golightly, Alan Klotz, Thomas Erik Mathisen, Claus Dambmann, Kimberly M. Brown
Abstract: The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
November 13, 1998
Date of Patent:
February 6, 2001
Assignees:
Novo Nordisk Biotech, Inc., Novo Nordisk A/S, Japan Tobacco, Inc.
Inventors:
Alexander Blinkovsky, Tony S. Byun, Alan V. Klotz, Alan Sloma, Kimberly Brown, Maria Tang, Mikio Fujii, Chigusa Marumoto
Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases.
Type:
Grant
Filed:
May 20, 1999
Date of Patent:
January 30, 2001
Assignee:
Novo Nordisk Biotech, Inc.
Inventors:
John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
Abstract: The present invention relates to isolated polypeptides having phospholipase B activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Abstract: The present invention relates to methods for dyeing a material, comprising (a) treating the material with a dyeing system which comprises one or more reduced vat dyes and/or one or more reduced sulfur dyes; and (b) oxidizing the one or more reduced vat dyes or one or more reduced sulfur dyes adsorbed onto the treated material with an oxidation system comprising (i) an oxygen source and one or more enzymes exhibiting oxidase activity or (ii) a hydrogen peroxide source and one or more enzymes exhibiting peroxidase activity, to convert the one or more reduced dyes to their original oxidized insoluble colored forms; wherein the material is a fabric, yarn, fiber, garment or film made of cotton, diacetate, flax, fur, hide, leather, linen, lyocel, polyacrylic, polyamide, polyester, ramie, rayon, silk, tencel, triacetate, viscose or wool.
Type:
Grant
Filed:
August 24, 1999
Date of Patent:
October 10, 2000
Assignee:
Novo Nordisk Biotech, Inc.
Inventors:
Feng Xu, Sonja Salmon, Heinz-Josef Wilhelm Deussen, Henrik Lund
Abstract: The present invention relates to methods of producing hemoproteins comprising (a) introducing into a filamentous fungal cell, which is capable of producing the hemoprotein, (i) one or more first control sequences capable of directing the expression of a heme biosynthetic enzyme encoded by a first nucleic acid sequence endogenous to the filamentous fungal cell, wherein the one or more of the first control sequences are operably linked to the first nucleic acid sequence; and/or (ii) one or more copies of one or more second nucleic acid sequences encoding a heme biosynthetic enzyme; (b) cultivating the filamentous fungal cell in a nutrient medium suitable for production of the hemoprotein and the heme biosynthetic enzymes; and (c) recovering the hemoprotein from the nutrient medium of the filamentous fungal cell.
Type:
Grant
Filed:
June 9, 1997
Date of Patent:
August 8, 2000
Assignee:
Novo Nordisk Biotech, Inc.
Inventors:
Susan L. Elrod, Joel R. Cherry, Aubrey Jones
Abstract: The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
Abstract: The present invention relates to isolated polypeptides having choline oxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
November 24, 1998
Date of Patent:
May 16, 2000
Assignee:
Novo Nordisk Biotech, Inc.
Inventors:
Debbie S. Yaver, Randy M. Berka, Michael W. Rey
Abstract: The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium host cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
Type:
Grant
Filed:
March 13, 1997
Date of Patent:
May 9, 2000
Assignee:
Novo Nordisk Biotech, Inc.
Inventors:
John C. Royer, Donna L. Moyer, Yoder T. Wendy, Jeffrey R. Shuster