Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing filamentous fungal expressed sequenced tags. The present invention also relates to filamentous fungal expressed sequenced tags and to computer readable media and substrates containing such expressed sequenced tags for monitoring expression of a plurality of genes in filamentous fungal cells.
Type:
Grant
Filed:
August 29, 2003
Date of Patent:
March 6, 2007
Assignee:
Novozymes, Inc.
Inventors:
Randy M. Berka, Michael W. Rey, Jeffrey R. Shuster, Sakari Kauppinen, Ib Groth Clausen, Peter Bjarke Olsen
Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.
Type:
Grant
Filed:
April 12, 2001
Date of Patent:
February 20, 2007
Assignee:
Novozymes, Inc.
Inventors:
William Widner, Alan Sloma, Michael D. Thomas
Abstract: The present invention relates to isolated polypeptides having Family 74 xyloglucanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
December 6, 2005
Date of Patent:
February 6, 2007
Assignee:
Novozymes, Inc.
Inventors:
Michael W. Rey, Elizabeth J. Zaretsky, Jeffrey A. Haas
Abstract: The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium host cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
Type:
Grant
Filed:
December 15, 1999
Date of Patent:
January 16, 2007
Assignee:
Novozymes, Inc.
Inventors:
John C. Royer, Donna L. Moyer, Jeffrey R. Shuster, Yoder T. Wendy
Abstract: The present invention relates to isolated polypeptides having dipeptidyl aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
November 25, 2003
Date of Patent:
August 29, 2006
Assignee:
Novozymes, Inc.
Inventors:
Alexandeer Blinkovsky, Kimberly Brown, Michael W. Rey, Alan Klotz, Tony Byun
Abstract: The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
Type:
Grant
Filed:
March 4, 2005
Date of Patent:
August 15, 2006
Assignees:
Novozymes, Inc., Novozymes A/S
Inventors:
Alexander Blinkovsky, Randy Berka, Michael Rey, Elizabeth Golightly, Alan Klotz, Thomas Erik Mathisen, Claus Dambmann
Abstract: The present invention relates to isolated polypeptides having oxaloacetate hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
July 29, 2005
Date of Patent:
June 6, 2006
Assignee:
Novozymes, Inc.
Inventors:
Debbie Yaver, Barbara Cherry, Jeffrey Murrell
Abstract: The present invention relates to methods for producing consumable products from potatoes, comprising: (a) treating a potato substance with an effective amount of one or more exogenous enzymes selected from the group consisting of an amyloglucosidase, glucose oxidase, laccase, lipase, maltogenic amylase, pectinase, pentosanase, protease, and transglutaminase, and (b) processing the enzyme-treated potato substance to produce a potato product. The invention also relates to consumable products obtained from potatoes by the methods of the present invention.
Type:
Grant
Filed:
April 13, 2001
Date of Patent:
June 6, 2006
Assignees:
Novozymes, Inc., Novozymes A/S
Inventors:
Feng Xu, Lene Venke Kofod, Hans Sejr Olsen
Abstract: The present invention relates to isolated polypeptides having Family 74 xyloglucanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
April 18, 2003
Date of Patent:
April 25, 2006
Assignee:
Novozymes, Inc.
Inventors:
Michael W. Rey, Elizabeth J. Zaretsky, Jeffrey A. Haas
Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.
Type:
Grant
Filed:
October 5, 2001
Date of Patent:
March 28, 2006
Assignee:
Novozymes, Inc.
Inventors:
Randy M. Berka, Ib Groth Clausen, Alexandre Bolotine, Alexei Sorokine, Alla Lapidus
Abstract: The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
Type:
Grant
Filed:
November 13, 2000
Date of Patent:
October 4, 2005
Assignees:
Novozymes Biotech, Inc., Novozymes A/S
Inventors:
Alexander Blinkovsky, Randy Berka, Michael Rey, Elizabeth Golightly, Alan Klotz, Thomas Erik Mathisen, Claus Dambmann
Abstract: The present invention relates to methods for isolating a gene encoding an enzyme, comprising: (a) adding a mixture of labeled first nucleic acid probes from a microbial strain cultured on medium without the substrate, and labeled second nucleic acid probes from a microbial strain cultured on medium with the substrate, to an array of random nucleic acid fragments of the microbial strain where the labeled nucleic acids hybridize to complementary sequences of the genomic fragments in the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter; (b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot in the array; and (c) isolating a gene from the microbial strain that encodes an enzyme that degrades the substrate. The present invention also relates to isolated genes obtained by such methods.
Abstract: The present invention relates to isolated polypeptides having oxaloacetate hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
August 6, 2004
Date of Patent:
September 6, 2005
Assignee:
Novozymes, Inc.
Inventors:
Debbie Yaver, Barbara Cherry, Jeffrey Murrell
Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
Type:
Grant
Filed:
November 10, 2000
Date of Patent:
June 7, 2005
Assignee:
Novozymes Biotech, Inc.
Inventors:
John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
Abstract: The present invention relates to methods for producing a heterologous polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the heterologous polypeptide, wherein (i) the mutant cell comprises a nucleic acid sequence encoding the heterologous polypeptide and (ii) the mutant produces less of the cyclohexadepsipeptide than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the heterologous polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated cyclohexadepsipeptide synthetases and isolated nucleic acid sequences encoding the cyclohexadepsipeptide synthetases.
Type:
Grant
Filed:
January 13, 2000
Date of Patent:
May 17, 2005
Assignee:
Novozymes Biotech, Inc.
Inventors:
Randy M. Berka, Michael W. Rey, Wendy T. Yoder
Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
May 15, 1998
Date of Patent:
October 5, 2004
Assignee:
Novozymes Biotech, Inc.
Inventors:
Alexander Blinkovsky, Kimberly Brown, Elizabeth Golightly, Tony Byun
Abstract: The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
Abstract: The present invention relates to methods for preventing or removing biofilm on a surface, comprising contacting the surface with an effective amount of a composition comprising one or more acylases and a carrier to degrade a lactone produced by one or more microorganisms, wherein the degradation of the lactone prevents or removes the biofilm.
Abstract: The present invention relates to isolated polypeptides having lactonohydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides. The present invention further relates to methods for preventing microbial biofilm development.
Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO. 1, nucleotides 1 to 938 of SEQ ID NO. 2, and nucleotides 1 to 3060 of SEQ ID NO. 3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypeptides.
Type:
Grant
Filed:
October 28, 2002
Date of Patent:
February 17, 2004
Assignee:
Novozymes Biotech, Inc.
Inventors:
Randy M. Berka, Michael W. Rey, Kimberly Brown, Stephen H. Brown